LOCUS       NZ_CP033210            11916 bp    DNA     circular CON 18-DEC-2023
DEFINITION  Enterococcus faecium strain RBWH1 plasmid pRBWH1.4, complete
            sequence.
ACCESSION   NZ_CP033210
VERSION     NZ_CP033210.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN10273305
            Assembly: GCF_003957785.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11916)
  AUTHORS   Bohlmann,L., De Oliveira,D.M.P., El-Deeb,I.M., Brazel,E.B.,
            Harbison-Price,N., Ong,C.Y., Rivera-Hernandez,T., Ferguson,S.A.,
            Cork,A.J., Phan,M.D., Soderholm,A.T., Davies,M.R., Nimmo,G.R.,
            Dougan,G., Schembri,M.A., Cook,G.M., McEwan,A.G., von Itzstein,M.,
            McDevitt,C.A. and Walker,M.J.
  TITLE     Chemical Synergy between Ionophore PBT2 and Zinc Reverses Antibiotic
            Resistance
  JOURNAL   mBio 9 (6), e02391-18 (2018)
   PUBMED   30538186
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11916)
  AUTHORS   Bohlmann,L., De Oliviera,D., El-Deeb,I., Brazel,E.,
            Harbison-Price,N., Ong,C.-L., River-Hernandez,T., Ferguson,S.,
            Cork,A., Phan,M.-D., Davies,M., Nimmo,G., Dougan,G., Schembri,M.,
            Cook,G., McEwan,A., von Itzstein,M., McDevitt,C. and Walker,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2018) Department of Microbiology and Immunology,
            University of Melbourne, The Doherty Institute of Infection and
            Immunology, Melbourne, Victoria 3000, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: PacBio SMRT Pipe v. 2.3.0
            Assembly Name                     :: RBWH1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 105.0x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 12/17/2023 02:36:20
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 92
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 44 of 123
            Pseudo Genes (incomplete)         :: 77 of 123
            Pseudo Genes (internal stop)      :: 24 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP033210.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..11916
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="RBWH1"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="pRBWH1.4"
                     /geo_loc_name="Australia: Brisbane"
                     /collection_date="2016"
                     /collected_by="Royal Brisbane Women's Hospital"
     gene            94..474
                     /locus_tag="EBB55_RS16085"
                     /old_locus_tag="EBB55_16080"
     CDS             94..474
                     /locus_tag="EBB55_RS16085"
                     /old_locus_tag="EBB55_16080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_010782660.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEALQ
                     MSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPGDA
                     QRLSENLKIMQKELQKIWQQLK"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            456..1370
                     /locus_tag="EBB55_RS16090"
                     /old_locus_tag="EBB55_16085"
     CDS             456..1370
                     /locus_tag="EBB55_RS16090"
                     /old_locus_tag="EBB55_16085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_010782659.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIRM
                     LYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHNHI
                     VINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKLKEK
                     NEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKKKVRS
                     NKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTTEQPEI
                     RPTRRNHDQHVERGADYQQELSF"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            1330..1608
                     /locus_tag="EBB55_RS16095"
                     /old_locus_tag="EBB55_16090"
     CDS             1330..1608
                     /locus_tag="EBB55_RS16095"
                     /old_locus_tag="EBB55_16090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002369881.1"
                     /translation="MKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTTIDSQ
                     VKKFHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
                     /name="hypothetical protein"
     gene            1615..2304
                     /locus_tag="EBB55_RS16100"
                     /old_locus_tag="EBB55_16095"
     CDS             1615..2304
                     /locus_tag="EBB55_RS16100"
                     /old_locus_tag="EBB55_16095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002369882.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLPG
                     VIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTTCY
                     EEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQTEEA
                     CYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKSVKEW
                     LDQQQQN"
                     /name="hypothetical protein"
     gene            3148..4092
                     /locus_tag="EBB55_RS16115"
                     /old_locus_tag="EBB55_16110"
     CDS             3148..4092
                     /locus_tag="EBB55_RS16115"
                     /old_locus_tag="EBB55_16110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_002303559.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVRF
                     TFDQLKDLSNYKATANVRFVEDLKATYEKILSLRFGRRSESGLSYDMFVMFTEFKINGD
                     VENPYVDIRIYEKALPLLNNLDEWVRYSLQQFNELQSGYAKTMFRLLKQYRTKGFAYFS
                     KEDFLELLDIPKSYKQPDIDKRVIKPIRQELTAIFKGLTIKKKYGKGRGKPVIGYQFTF
                     KPEIKKADDFNKHAPTKEDQNKALALADKQKEYEEPATTINEKIKQKKRELELLQQIKQ
                     LEKEKKALEKQTESALSEKEKKSLLSDLDNLFQ"
                     /name="replication initiation protein"
     gene            4113..4646
                     /locus_tag="EBB55_RS16120"
                     /old_locus_tag="EBB55_16115"
     CDS             4113..4646
                     /locus_tag="EBB55_RS16120"
                     /old_locus_tag="EBB55_16115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_002363576.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKVV
                     LRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLREQL
                     ALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATETMD
                     EQTKNWKKWWQLWK"
                     /name="DUF536 domain-containing protein"
     gene            4661..4831
                     /locus_tag="EBB55_RS16510"
                     /old_locus_tag="EBB55_16120"
     CDS             4661..4831
                     /locus_tag="EBB55_RS16510"
                     /old_locus_tag="EBB55_16120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032491833.1"
                     /translation="MTKKHLLFGSILLGILSFSPMLLSVANKGNKKGELKLNPNYYNSQ
                     NEEVFVEKLDK"
                     /name="hypothetical protein"
     gene            5286..5510
                     /locus_tag="EBB55_RS16125"
                     /old_locus_tag="EBB55_16125"
     CDS             5286..5510
                     /locus_tag="EBB55_RS16125"
                     /old_locus_tag="EBB55_16125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="WP_002363584.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEKC
                     WVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
                     /name="class II bacteriocin"
     gene            5530..5874
                     /locus_tag="EBB55_RS16130"
                     /old_locus_tag="EBB55_16130"
     CDS             5530..5874
                     /locus_tag="EBB55_RS16130"
                     /old_locus_tag="EBB55_16130"
                     /inference="COORDINATES: protein motif:HMM:NF020523.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="WP_236095792.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKSV
                     GEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIFTVYYTHIRDHET
                     GAYIVIPNNK"
                     /name="bacteriocin immunity protein"
     gene            6923..7891
                     /locus_tag="EBB55_RS16140"
                     /old_locus_tag="EBB55_16140"
     CDS             6923..7891
                     /locus_tag="EBB55_RS16140"
                     /old_locus_tag="EBB55_16140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347614.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_002347614.1"
                     /translation="MSNEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMRDVGDKKVQF
                     TFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKIDGK
                     SDIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGFAYFS
                     KEDFHELLDIPKSYNQGTIDQRVFNPIRQELTAIFKGLTIKKKYGKGRGKPVIGYQFTF
                     KPEIKKSDDFNKHAPTKEEQNKALELANQQVQSEKAPTSLQEKIKEQKRKLELLQQLEK
                     LEREQNTQNKENNAQKSEINSDIPSELKKDLLDNLQNLFKD"
                     /name="replication initiation protein"
     gene            7908..8432
                     /locus_tag="EBB55_RS16145"
                     /old_locus_tag="EBB55_16145"
     CDS             7908..8432
                     /locus_tag="EBB55_RS16145"
                     /old_locus_tag="EBB55_16145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_044383356.1"
                     /translation="MTEKTIKELAEELGVSKTAINKKVSDENRKLWFSKIGNKFVINET
                     GQKAIKSMFFNENENSNQQLVGEKTKTENHKNANQVPELEVLSLLEQQLNQKDMQLQEK
                     DEQIKQMQKLLDHQQQLTLQANQQIEKLQEQLQLTYTEESSENKSTTLSEETESIEKQS
                     KAEKKWWQFWQ"
                     /name="DUF536 domain-containing protein"
     gene            8808..9089
                     /locus_tag="EBB55_RS16150"
                     /old_locus_tag="EBB55_16150"
     CDS             8808..9089
                     /locus_tag="EBB55_RS16150"
                     /old_locus_tag="EBB55_16150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000353845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002347613.1"
                     /translation="MDILVRNINPKIIARIDKRCQELTTKTGKKWSRNKYLKVLIESDF
                     DRQLMNYKKDQFDLLLEKFIAIQTENTNILNDYVHTNNQLIETLIGGI"
                     /name="hypothetical protein"
     gene            9091..9507
                     /locus_tag="EBB55_RS16155"
                     /old_locus_tag="EBB55_16155"
     CDS             9091..9507
                     /locus_tag="EBB55_RS16155"
                     /old_locus_tag="EBB55_16155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002305824.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDVNNYSEAIRFLCMSYE
                     PDTNTTTTSNQAKLNAMGKEISIMTEIMAEFADLYLKDTGVLIGTNSKVYQEVKEKIEN
                     QLNPSRRNQPKNRQKETNQTFTSGNFDPTNFLKR"
                     /name="hypothetical protein"
     gene            10183..11283
                     /locus_tag="EBB55_RS16160"
                     /old_locus_tag="EBB55_16160"
     CDS             10183..11283
                     /locus_tag="EBB55_RS16160"
                     /old_locus_tag="EBB55_16160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine protease staphopain"
                     /protein_id="WP_224198038.1"
                     /translation="MIGIFSIGITNTYASENSNFNINSQPIEEQAINFAKTDFEHSITS
                     ILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFSDADNTIKYIYTVKKNTNGEY
                     SGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSDNGQIKLLWKSPLSTEKNGVSSFT
                     EKNDLISVNIKNKIKNFSKKQNFPAVKRVKSVSSGDNFIMINWRIAETQTSFPWCAAYV
                     EAAILCNKTDVRATSARAIMHYTYPNLNNSQIESKSLNQDQIIKYANYCGVYPKKVARI
                     LNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHALALFGWVNQGGYQTYYVWNPWYNYPTV
                     ISANAGPVTIPVPGGGFTWDNTITNW"
                     /name="cysteine protease staphopain"
     gene            11256..11699
                     /locus_tag="EBB55_RS16165"
                     /old_locus_tag="EBB55_16165"
     CDS             11256..11699
                     /locus_tag="EBB55_RS16165"
                     /old_locus_tag="EBB55_16165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002318191.1"
                     /translation="MGQYHYKLVNKPIALVMGLVLVLFLTSCSKKQEIQKTDLIPFLGT
                     WQTKDHSERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNLLT
                     LRSNKKQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
                     /name="hypothetical protein"
     BGC             286..10510
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          3149..4092
                     /name="rep_cluster_1197"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          6924..7887
                     /name="rep_cluster_1093"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.782"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          457..1367
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.421"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gtgagcttta gtgagcttta cttattgtac ctttttaggt acaatggtat tgcgaacgat
       61 acgtccgtag gacgtactga aagggtggga cttatgagcg aacaaaatca atatttggct
      121 agcaatcatt ctaaaaaata tacctatcga tccgagccaa aacaaatcag ttttcgagtg
      181 agcgaatccg aatttgcaaa gctgaagcag tcagctgaag ctttgcaaat gagtgtgccg
      241 gctttcgtga aagccaaggc acaaaacgcg cgtctggtga caccgaaagt ggcgccagac
      301 attgctcagg caatcgcgcg cgatttagcc aaagctggcg ggaatatcaa tcaaattgcc
      361 aagtggtgca acacacacca acacgacgtt gatcctggcg acgcccaacg cttatccgaa
      421 aacctaaaaa tcatgcaaaa ggagctacaa aagatatggc aacagttaaa gtaagccgaa
      481 ccacttcttg tagccgagcg atcaattacg ccgaaccacg agcaaccgtc aaaacaggga
      541 taaactgtga cattgattat gccaaaagcg aaatgaaaca aattcgcatg ctgtatggaa
      601 aagacgatcg tgtacaagca cacttattga tccagtcttt tcgaccagga gaaattaccg
      661 ctgaaaaagc caatcaacta ggggcagagt atgcgaaaaa aatagcccct gaccaccaaa
      721 tcgcgattta cacacataca gacaaagacc atattcataa ccacattgtc attaactccg
      781 tcaatctgga aactgggaac aaatttcaag cccatggtca agcattttta aacaaatgtt
      841 atgacctaaa tgacgagatt tgtcttgaac atgacttaac aatcactgaa cgagggaaga
      901 aacccgaaaa acgaaccatg tctgaaatca aattaaaaga aaaaaatgaa cctgtttgga
      961 aagacgaaat tcggtttgcg attgatcaaa caatgaaaaa caaaaaaact cgaacgtata
     1021 accaattttg tgattctctt aaaacattcg gcattcattg cttgaatcgt gggaaaaatt
     1081 ttacttacga attgatccac aaaaagaaaa aagtacgatc caacaaacta ggaaaggatt
     1141 acgaaaagga gacgatttta catgagctgg acagacgaga acgagcctac aatcaacaac
     1201 gaaccaacga gctacgaaga gctcgagcta gcattcgcga aactgcaaaa gaagtacgaa
     1261 gagaacaacg aactcttacg actgaacaac cggaaattag accgactcga agaaaccatg
     1321 accaacacgt tgaaagagga gcagactacc aacaagaatt atcgttctaa aacgttacaa
     1381 gacatagaaa atgaaataaa gaaacaacgt gcttttcttg caccttatac caaagaaaga
     1441 atacaaacta caattgattc acaagtcaaa aaattccacc atgcgctatt aaaccagcga
     1501 aataaagaac agaaaaaagt aattatgcag cgatcaatgg atattcttgc ccccttatgt
     1561 tgcttaatca tgacactgtt agccttttgg ctggtatggg gagaataaat agaaatggca
     1621 ttctatttac cctatctact catttttgcc agtattttag gaattatttg gcttagctat
     1681 aaggcttatc aaacaagata ttcattaaaa ggctctaaaa ttcgatttaa gaggttttta
     1741 ttgccagggg ttatatttac attatcaatc gttttaagtg gtttattcgg cgttttagag
     1801 ggaaataaac gtatttcaag aagtatttta ttaggggata tcactcaaaa gtatgaagca
     1861 gcatgtgata aaaagaaaaa ggagcaagtg ttaacacaaa aaatggaaga gtttaccact
     1921 tgttatgagg aaatgaacga tatttttgtg aatcaagaaa aacgtttgac ggataaaaac
     1981 atggaaaaac tcactcgtct ttatcaaaac ttgccagaaa aacagcaaaa agaggttcaa
     2041 gacaactatg agcgaaccaa aaaagacgtg caatatttta aagatacgca gacagaagaa
     2101 gcttgttata atttattcag tgatacaatc cctttttcaa ctagtgaaca ggaacgaaaa
     2161 gaacggcaac aatcagttac ctacgaacgt tataaagcac tgcttcagca agcaacgaac
     2221 atacaaaatc caacgaaaaa agaaacggca ttaaattatc taaaaagtgt caaggaatgg
     2281 ttagatcagc aacagcaaaa ttgaatttga tcggcaatca tgttaaacta aacatgcaaa
     2341 tgatcaaaca ttagatacac gaaagcccca gagaggtcgg attctctggg gctttttttc
     2401 aggttgatgt cgcctttatt gattttactt cttgtttcgg ttattcaacc aataactgaa
     2461 taaagagaca caaacttcta taagaagggg gcaaatgaac aattcaaaca ttagatacac
     2521 ctccttccag tcgcaagact gtcggatagg cgacaagtta ttatatctta aaaaattaaa
     2581 cttgtgtggt gtttttaagt atcgcttatt ttagggttgt tagggcttgc cctgaccgcc
     2641 tgtaaggcgc tttaaagcta gataaggatt tttatccaag actgtcttaa aacggcttag
     2701 ataagcattc agaaccgctc aaaggcctct ttaccaacat gtttttagca aaggagcctt
     2761 taggcgactg cgcagtacaa tgtgagagcg tagcgatcac acctttttgg cgaccgtagg
     2821 aagcaaggtt ttggattggc agttttgaaa aatgcgcgaa cctgtttaaa gaaaaaagaa
     2881 gtggttttta ggcgggaaaa ttagcccccc tctttttata tctcttttta aacctctttt
     2941 taaacctctt taccgtaagc tgtgtcctta ctctaccaat ggatacagaa tgttatgaag
     3001 gacattttgt ctgtttatga aggacatttt gtctgtttat gaaggacatt ttgtctgttt
     3061 atgaaggaca ttttgtctgt ttgaacgtca aatatgaatg tcttgacatt taaaaaatgt
     3121 cattcataat tggattgagg tgattatatg gctaatgaga ttgtaaaata ccaccatgaa
     3181 ttaaatacaa ttcctttaag aaaatttaca ccggttgaaa tgaatttgtt tttttccatc
     3241 gtgtctagaa tgcgtgaaaa aggggataag acagttcgtt ttacgtttga ccaactaaaa
     3301 gatttaagta actacaaagc aactgcaaat gtacgatttg tagaagattt aaaagcaact
     3361 tatgaaaaaa tcttatcttt acgctttggt cgtagaagtg agtctgggct ttcttacgat
     3421 atgtttgtaa tgtttacaga atttaaaatc aacggggacg tagaaaatcc ttacgtagat
     3481 atacgaattt acgaaaaagc actgcctttg ttaaataatt tagacgagtg ggtacgctat
     3541 tctttacaac aattcaacga gctacaaagt ggctatgcaa agactatgtt tcgtctgtta
     3601 aagcaataca gaaccaaagg tttcgcatat ttctccaaag aagactttct tgaattatta
     3661 gatattccta aaagttataa gcaacctgat atagataaac gagttataaa acctatacgt
     3721 caagaactaa ctgcaatctt caaaggacta acaatcaaaa agaaatacgg aaaaggacgg
     3781 ggaaaacctg ttattggcta tcaatttacg ttcaaacctg agatcaaaaa agcagacgac
     3841 tttaacaaac atgcaccaac caaagaagac caaaataaag cattggcttt agctgataaa
     3901 caaaaagaat acgaagaacc cgctacaaca attaacgaaa aaatcaaaca gaagaaacga
     3961 gaacttgaac tcctccaaca aatcaaacaa ctagaaaaag agaaaaaagc tcttgaaaaa
     4021 caaacagagt ccgcactttc ggaaaaagag aaaaaaagtt tattatctga tttagataat
     4081 ttattccagt agcaacctag gaggcaacca gtatgcaacc acaagaaacc tttacaataa
     4141 aggaattgtc agatcttttt aagatgagta ggcaagcaat atcgaaacat atccaaaaat
     4201 tagattcttc catgatagct aaaaatgagc gtggttataa ggttgtattg cgttctggag
     4261 tattgcaact tgctcgcaac ttagacaatc aaaggttgct agataagttg cataaacatg
     4321 aacaaaacaa cgataaaaat gcttatatac caaacaaaaa cgtggttgca aacaagttgc
     4381 tagatcagtt gcgtgaagaa aatgaatttt tacgagaaca acttgccctc aaagagcaaa
     4441 ataattctca tatacaaaaa ctactcgacc agcaacaaca actcaccctg caagccaacc
     4501 aacaaatcga aaaacttcag gagcaactgc agttgactta tacggaagag tcttcagaga
     4561 atagttcaac gactctttct gaagcaacag aaacaatgga cgaacaaaca aagaattgga
     4621 aaaaatggtg gcagctatgg aaatgaagaa ggaaatactt atgacaaaaa aacacttgct
     4681 attcggttct atcctactag gaatattaag ctttagccca atgttattat cagtggccaa
     4741 caaaggaaat aaaaaagggg aactaaaact caatcctaat tactacaaca gtcaaaatga
     4801 ggaagtattt gtagaaaaac ttgataaata agaaaagact gctcgtacga gcagtctttc
     4861 tgaaagagat tatcctatta ttaaccttag aaaagaataa taaatgaata tgagatatgt
     4921 aaaaagcata acaaacacaa acaaaaataa caagtattta aaaaatgacc gatcaatatt
     4981 cacaaagaat tagaaagaaa caaccgcaga aaaccaagcc ttaaaaagag cttttaacct
     5041 agaccaataa aaattcacaa aatcagcgcc tggatccctt ggtacaggcg ttacttggta
     5101 gtatacacat tatgtctact agctgtttaa ataactgatc ttataatttt atttaaacga
     5161 ttatgaattc aaaactactt tttatgacgt taatcattgt tatataataa aatgttatat
     5221 aataaaatta ctaagattat ttaatataac aagcaatttt aaaaaaacga aaaggaaaaa
     5281 caatcatgaa aaagaaagta ttaaaacatt gtgttattct aggaatatta ggaacttgtc
     5341 tagctggcat cggtacagga ataaaagttg atgcagctac ttactatgga aatggtcttt
     5401 attgtaacaa agaaaaatgt tgggtagatt ggaatcaagc taaaggagaa attggaaaaa
     5461 ttattgttaa tggttgggtt aatcatggtc catgggcacc tagaaggtag tggtagggag
     5521 gttattataa tggattttac taaagaagaa aaacttttaa atgcaattag taaagtatac
     5581 aatgaagcaa ctatagatga ctatcctgac ttaaaagaaa agctctttct ttattctaaa
     5641 gaaatcagtg agggaaaaag tgttggtgaa gttagtatga aattaagtag ttttcttgga
     5701 agatatattt taaaacataa atttggatta cctaaatctt taatagaatt acaagaaatt
     5761 gttagtaagg aatctcaagt atatagagga tgggcttcta tttttactgt atattataca
     5821 catatccgag accacgagac aggagcatat atagtaatac ctaataataa ataaaaaaaa
     5881 actatgagct tgcgcccctt tgacaagcgg ttatttgcac caaatacgta ggtatttggc
     5941 tagcaaatat agagcttgcc aaacacttta gtgagttttg tagaaagtac ctttaaaggt
     6001 actttcacat tacgaacgat acgtccgtag gacgtactga aagggtggga tttatgagcg
     6061 aactaatgca aaatttggct agcaaaaaaa gttaaaaaat atatgctaat aatcgagcga
     6121 aatgaacact ttttcaagtg agcgaatccg ccatgacaaa gctgaagcag tttatttcag
     6181 ctttgtcatg gcgtgtgcct agacttttaa aaaagtcggc acacgagcaa atatctttat
     6241 caagctctaa tttgcgtttt aagacgtttg aaacgtctaa gtatataaat atataagagt
     6301 aggtttaaga gtgcttaaat cgaattttag agggcattca gacaacttta gggttgttag
     6361 ggcttgccct gaccgcctgt aaggcgcttt aagaacaaga taagggattt atactaagac
     6421 tgtcttaaaa cgtctcaaac gaaggctcat agcttctcaa agagttcctt atgaacattt
     6481 ttagcaaagg agccggaagc gactgcgcag tataatgtga gagcagagcg atcacacctt
     6541 tttaagcgac tgtagggagt aagattccca gttgagaaaa atgcgcgaat ctgtttggag
     6601 aaaaaagaag tggtttttta ggcgggaaat tttgtccccc tcttttataa tctcttttta
     6661 aacctctttt taaacctctt taccgtcggc tatatcctta ctctcccaag ggaatgacgc
     6721 ttttatctac atcgttttgt ctgtttatct acatcgtttt gtctgtttat ctacatcgtt
     6781 ttgtctgttt atctacatcg ttttgtctgt ttgtacattg aattagtaca tcgacgatgt
     6841 taatataaga tgtagataaa caggcatttt gtatttcata acgtgtatct cttgtgcgag
     6901 taagaaagga gctattttaa atatgtctaa tgaaatcgta aagtatcatc atgaattaaa
     6961 tacaattccg ttgcgaaaat ttacaccagt cgaaatgaat ttgttctttt ctatcgtatc
     7021 aagaatgcgt gatgtaggtg ataaaaaagt acaatttaca ttcgagcaat taaaagattt
     7081 aagcaattat aaagcaactg caaatgttcg attcatagat gatttagaaa caacgtatga
     7141 caaattaatg gatttacgtt ttggtagaag aagtgctgac ggtttgcaac gtgaacgttt
     7201 tgttttattt aatcaattta aaatcgacgg aaaatctgat attccttttg ctgaaataca
     7261 agttcatgaa aaagccttac ccttacttaa taatcttgaa gaatgggtgc gctattctct
     7321 ccaacaattc aatgaattag aaagcagtta ttcaaaaaca atgtttcgat tactaaaaca
     7381 atacagaacc aaaggtttcg cttatttttc aaaagaagac tttcatgaat tacttgatat
     7441 tcctaaaagt tacaaccaag gtacgattga tcaacgtgtg ttcaatccaa tacgacaaga
     7501 actaaccgca atctttaaag gtttaacaat caaaaagaaa tacggaaaag gacggggaaa
     7561 gcctgtcatt ggctatcaat ttacatttaa acctgagata aaaaaatcag acgactttaa
     7621 caaacatgcc ccaacaaaag aagaacaaaa caaagcatta gaactagcta accaacaagt
     7681 tcaatctgaa aaagcaccta cttcattaca agaaaaaatc aaagaacaaa agcgaaaatt
     7741 ggaactttta caacagttag aaaagttaga aagagaacaa aatacccaaa ataaagaaaa
     7801 taatgcacaa aaatcagaga taaattccga tattccttca gaactaaaaa aagacttatt
     7861 agacaatctt caaaacttat tcaaagattg aggggttttt agattttatg acagaaaaaa
     7921 caatcaaaga acttgctgaa gaattaggag ttagtaaaac agcaattaac aaaaaagttt
     7981 ctgacgaaaa tcgaaaactt tggttttcga aaatcggaaa caagtttgtg attaatgaaa
     8041 ccggacaaaa ggctataaaa tcaatgtttt ttaacgaaaa cgaaaactca aatcaacaac
     8101 tagttggcga aaaaacgaaa accgaaaacc acaaaaacgc caaccaagtt cccgaattag
     8161 aagtcctttc tctcctagaa caacagttaa atcaaaaaga tatgcaacta caagaaaaag
     8221 acgagcaaat aaaacaaatg caaaaattac tcgaccacca acaacaactc accctgcaag
     8281 ccaaccaaca aatcgagaaa ctgcaggaac aactacagtt gacttatacg gaagaatctt
     8341 cggaaaataa atcaacgact ctttctgaag aaacagaatc gatagaaaaa caatcaaagg
     8401 cggagaaaaa atggtggcaa ttctggcaat aaaaccagca atttaaatta ataaaaattg
     8461 ctggttttat tcttcttttt gcttttgcga gcagagcaac atggacccat gttgcgatat
     8521 tttggtggcg ccccaatccc cgttttttcg ttgaatctta ctcggactgc actcgtttta
     8581 cagctgattt ttctgaattt tgaaagagca taaggaaacg tggtatcact ttcttacgaa
     8641 aggtgctaga taggctgtgc ctatcacgtt tctcagaaaa aaatttgcta tcactttttt
     8701 gccattgtgg ctaggctatg tatagtgttt tggcgaaaaa tagctataca aatactatat
     8761 ttgtatggta tacataaatt atacgctgat acgaaaggaa aatttccatg gacattttag
     8821 ttcgaaatat caatcccaaa attattgcaa gaatagataa acgttgtcag gaactcacaa
     8881 ccaaaacggg caaaaaatgg agtcgaaata aatatctaaa agttctaatc gaaagtgatt
     8941 ttgaccgtca acttatgaat tacaaaaaag atcaattcga tttattactc gaaaaattta
     9001 ttgccattca aacagaaaat acgaacatac tgaatgacta tgttcacaca aacaaccaac
     9061 tcattgaaac acttatcgga ggaatataac atgagaaaaa cttctgttca aaaacacgta
     9121 accctttatc ccagtcacgt tgacgtttta gattccatca tcgaaaaaaa tccagacgtc
     9181 aacaattatt cagaagccat tcgtttttta tgtatgagtt atgaacctga taccaataca
     9241 accacaacca gtaaccaagc aaaactcaat gccatgggaa aagaaatttc tatcatgaca
     9301 gaaatcatgg cagaatttgc tgatttatat ctaaaagata ctggtgttct tatcggtaca
     9361 aattctaaag tgtatcaaga agtgaaagaa aaaattgaaa atcaactcaa tccttcccga
     9421 cgaaatcaac ccaaaaatcg tcaaaaagaa accaatcaaa cctttacttc tggaaatttt
     9481 gacccaacaa actttctaaa aagataaaaa aagaaaccga tccctaaaaa aattcctcga
     9541 tttcttttta gggatcagtc ttccagcaag atttgcaagt tggtaccaac ttgcgaaaaa
     9601 aaagaaaaat ccgcttatgc aacttcttct ttttgtattt tgaggcgcta ccccaatccc
     9661 caagtatcgg ttatttaata gcgcggaaat ccaaagaata ggacgtaccc tgaaacaaaa
     9721 aaagactgct ctcacgagca gcccaaaatg agattatcct attcatcaac ataaaaatat
     9781 tacaaagtga agaactagag aaagacaaaa aaagcatatc aaatcctctc aaaaataaca
     9841 agtctttaaa aaatgacaga taaaaatgca caaagaacta aaaaagagaa atcgcgtaaa
     9901 atcaagcatt agaagctagt aactagctag accaattaaa aatcacaaag tgagcgtctc
     9961 tatccattgg tagaggggct acttggcata gtgtacatta tgttaactaa cgctataaat
    10021 aacgattatg ttcccaaaac aatattttat gaactataac aaaaatttgt tatcgcatat
    10081 tgtatatttt aattataggt gtataccact taaatttaac acaaaggcgt gattattttg
    10141 aaacccaaaa agaaaattta tctaggtctc ctcttttcct tgatgattgg aatttttagt
    10201 ataggaatta caaataccta tgcttcagaa aattctaatt tcaatatcaa ctcccaacca
    10261 attgaagaac aagcaattaa ttttgcgaaa actgattttg aacactccat aacttcaatt
    10321 cttttaatgg aaaataacac cgaaactgat attaactata ctcttggttc gccatttaaa
    10381 atgctcgatg gagaaaatac ggcaaatagt tctattttct ttccagtatt ttcagacgct
    10441 gataatacga ttaaatatat ttataccgtc aaaaaaaata caaatggaga atattcagga
    10501 agtatttcac aatttttagc aaaagaactt caaaatctag tagatagtaa tataacagat
    10561 acaaccttat tttttcaaga taatagcatt tattattccg ataatggaca aattaaacta
    10621 ctttggaaat cacctttaag tacagaaaaa aatggtgttt cttcctttac agaaaaaaat
    10681 gatcttatat ccgtaaatat aaaaaataaa attaaaaatt tctctaaaaa acaaaatttt
    10741 cctgcggtta aacgtgtaaa aagtgtctcc tccggagata actttattat gattaattgg
    10801 cgtatagcag aaacccaaac aagttttccg tggtgtgctg cttacgtaga agctgctatc
    10861 ttatgtaaca aaacagatgt acgtgctact tcagctagag ccattatgca ttatacctat
    10921 cctaatttaa acaacagtca aattgaatca aaaagtctta atcaagatca aataataaaa
    10981 tatgcaaatt actgtggtgt ttatcctaaa aaagtagcac gaatactaaa cctttcagag
    11041 gtccaaacac aactaagaaa aggaaatgcc atttatatgg gaactgctgg ttatggaaat
    11101 tatagtaaca gccgtcatgc acttgcatta tttgggtggg tcaatcaagg tggttatcaa
    11161 acttactatg tgtggaatcc ttggtataac tacccaactg taataagtgc aaatgcagga
    11221 ccagttacaa ttcctgtacc tggaggtggt tttacttggg acaataccat tacaaattgg
    11281 taaataaacc cattgcttta gttatgggtc tagtgttagt tcttttctta actagttgtt
    11341 caaaaaaaca agaaatacaa aagactgatc tgattccttt tctaggaact tggcaaacta
    11401 aagatcattc agaacgtcta gtaatcacta atttaaatac ttcaaaaaat atagaacaat
    11461 ataccatctt agtaaaagag aacaatcata aaattcaaat cattgataaa aatttaaata
    11521 taaatgaaca agttttcatt tacaaagcaa caaataattt actaacactg cgctctaata
    11581 aaaaacaatt agattacact tttagtttgg attctccaaa aaagctcgta tttattttga
    11641 caacgaatga tgggacagaa gggacagcta aaccaattga attttttaaa gctgattgat
    11701 gaatagttgt ttgatattac aaaacaataa tttcaccgaa ggtgaaacaa gcgaaacttt
    11761 acaaaaagtg gctagcattt gctagccttt tttgtattgt ggagcgtggt ttttgagggg
    11821 gattcaaggg ggagcttgcg cccctttgac aagcggttat ttgcaccaaa tacgtaggta
    11881 tttggctagc aaatatagag cttgccaagc ccttta
//
