LOCUS       NZ_CP033132             8773 bp    DNA     circular CON 05-JAN-2024
DEFINITION  Acinetobacter wuhouensis strain WCHAW010062 plasmid pOXA653_010062,
            complete sequence.
ACCESSION   NZ_CP033132
VERSION     NZ_CP033132.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN07138954
            Assembly: GCF_002165345.2
KEYWORDS    RefSeq.
SOURCE      Acinetobacter wuhouensis
  ORGANISM  Acinetobacter wuhouensis
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter.
REFERENCE   1  (bases 1 to 8773)
  AUTHORS   Hu,Y., Long,H., Feng,Y. and Zong,Z.
  TITLE     The complete genome of Acinetobacter wuhouensis strain WCHAW010062
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8773)
  AUTHORS   Hu,Y., Long,H., Feng,Y. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-OCT-2018) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
REFERENCE   3  (bases 1 to 8773)
  AUTHORS   Hu,Y., Feng,Y. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2017) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/04/2024 23:19:32
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,443
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 4,234
            CDSs (with protein)               :: 4,234
            Genes (RNA)                       :: 107
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 27 of 102
            Pseudo Genes (incomplete)         :: 73 of 102
            Pseudo Genes (internal stop)      :: 16 of 102
            Pseudo Genes (multiple problems)  :: 14 of 102
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP033132.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8773
                     /organism="Acinetobacter wuhouensis"
                     /mol_type="genomic DNA"
                     /strain="WCHAW010062"
                     /isolation_source="Sewage"
                     /db_xref="taxon:1879050"
                     /plasmid="pOXA653_010062"
                     /geo_loc_name="China: Sichuan, Chengdu"
                     /collection_date="2015-10-31"
     gene            1..888
                     /gene="repM"
                     /locus_tag="CDG68_RS02860"
                     /old_locus_tag="CDG68_02865"
     CDS             1..888
                     /gene="repM"
                     /locus_tag="CDG68_RS02860"
                     /old_locus_tag="CDG68_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360254.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepM"
                     /protein_id="WP_012780181.1"
                     /translation="MDKNQIVKSNQVIEASYQLSAVEQRIVLAAISRIPKSQPITDDEL
                     YPVSVNELQLLGVHEKTAYRDLKEGINRLYERSINLSIDDKSIKMRWVQEIQFLDSQSI
                     IGIRFSKPILPFISNLSREFTKYALSDIAGINSGYGIRIYELLVQYRQIGKREISVENL
                     RSMLELGKKYPLFADFKKRVIDTAVDQINECSPLSVSYEQKKTGRKVTHIQFSFKEKSK
                     SINHLNEPEKFYKLTDAQVHMFGNQLSRLHELSHLATQGESYDDLAATIKEMLRDPIQQ
                     KQFIPHLRNLGFKG"
                     /name="repM"
     gene            965..1522
                     /locus_tag="CDG68_RS02865"
                     /old_locus_tag="CDG68_02870"
     CDS             965..1522
                     /locus_tag="CDG68_RS02865"
                     /old_locus_tag="CDG68_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017398013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication DNA-binding protein"
                     /protein_id="WP_012780177.1"
                     /translation="MKSLSVIELSNLYGMTRQAVYKQINKGNLSKNSDGKIDLSEAIRV
                     FGEPSKYVNSLQTTETRKLSEVHLLEQQVHMLQKQLEQAHEREKFQRDELKAKNDQLHV
                     KDEQIEAMQRLLEAPKTNMTTFADQKPSQDIATDPRPEPEPKHDGLTTPVEIENKRIPV
                     PEQVELEQPKRGWLSRFFLPNG"
                     /name="plasmid replication DNA-binding protein"
     gene            complement(1717..1992)
                     /locus_tag="CDG68_RS02870"
                     /old_locus_tag="CDG68_02875"
     CDS             complement(1717..1992)
                     /locus_tag="CDG68_RS02870"
                     /old_locus_tag="CDG68_02875"
                     /inference="COORDINATES: protein motif:HMM:NF015807.4"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="WP_075911389.1"
                     /translation="MIKKYTQVNFRLPLDLKEEIEQSASLTGNSITAEIVERLRNSFEY
                     DNLMLDNIELQSELINLESEKLDLLLEYQDKLCKIQDQILEELKKK"
                     /name="Arc family DNA-binding protein"
     gene            2086..2484
                     /locus_tag="CDG68_RS02875"
                     /old_locus_tag="CDG68_02880"
     CDS             2086..2484
                     /locus_tag="CDG68_RS02875"
                     /old_locus_tag="CDG68_02880"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_075911388.1"
                     /translation="MSNIIEFKRNERRFINQKPISPVELVTNILTPQEVDIKSLIKETK
                     KILREKGLKFRTSHQDLEHLKGNNLILFSKKKCGFILTTKLACSTNSSWEIYSISIDKT
                     TKALDIVNKIISRLGDNVHGSSREEAIS"
                     /name="hypothetical protein"
     gene            2453..2671
                     /locus_tag="CDG68_RS02880"
                     /old_locus_tag="CDG68_02885"
     CDS             2453..2671
                     /locus_tag="CDG68_RS02880"
                     /old_locus_tag="CDG68_02885"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_075911387.1"
                     /translation="MAAAEKKQSANPAMEAAKEAANIKIDGFEQGVNTYNAIGSQLLRE
                     KRDPSVINKTRENRRNIRKAREAIKNL"
                     /name="hypothetical protein"
     gene            complement(2760..3494)
                     /locus_tag="CDG68_RS02885"
                     /old_locus_tag="CDG68_02890"
     CDS             complement(2760..3494)
                     /locus_tag="CDG68_RS02885"
                     /old_locus_tag="CDG68_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005207180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_075911386.1"
                     /translation="MTPPHILLEENYNKPLIPLPVYFSYENILESKYFYNQREGGIFCL
                     KELQASEGSEYSGCIELFYCEFNNEYALDGVCAVADEYLDEYDAANKALEAYEVEKEIL
                     IARYAFKDIEIINDSIKITGKQIKGASILANYQRNGVSSFIYKYLLKKYGVLVCDNYQT
                     YKGHMLWVLSIVKLSVIRIYDLTKKEFIGTFDKVSPCLIKPWSVPYNFPSDKEKFLRLD
                     ACVYTELEFHSLVLVTFAEDMF"
                     /name="hypothetical protein"
     gene            complement(3513..3833)
                     /locus_tag="CDG68_RS02890"
                     /old_locus_tag="CDG68_02895"
     CDS             complement(3513..3833)
                     /locus_tag="CDG68_RS02890"
                     /old_locus_tag="CDG68_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005207183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_012780178.1"
                     /translation="MFTRTVQTLKNSTDLVQRFTMPNIRQTFELRRFSEKEKNKQYILI
                     FKDIILNKKDWDDVKVVAEIQERNNSLRFSIKASKQYPELTSYEKMLEAKINDIIKPTL
                     VA"
                     /name="hypothetical protein"
     gene            3984..4394
                     /locus_tag="CDG68_RS02895"
                     /old_locus_tag="CDG68_02900"
     CDS             3984..4394
                     /locus_tag="CDG68_RS02895"
                     /old_locus_tag="CDG68_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005207185.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_075911385.1"
                     /translation="MLNFELKEKWGENSLILGFTQIPTTLIYAQKELGLSSIEINILLN
                     LLTHWWKKEEFPYPSQAGIAYRMGVSTRTVQRTLAGLETKGFITRNKTSRDNSKYKGRS
                     IYDLSPLVKILEEKAPDLDIVKKIKKNKRLAK"
                     /name="helix-turn-helix domain-containing protein"
     gene            5109..5936
                     /locus_tag="CDG68_RS02905"
                     /old_locus_tag="CDG68_02910"
     CDS             5109..5936
                     /locus_tag="CDG68_RS02905"
                     /old_locus_tag="CDG68_02910"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_057689930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OXA-24 family carbapenem-hydrolyzing class D
                     beta-lactamase OXA-653"
                     /protein_id="WP_087554513.1"
                     /translation="MKKFILPIFSISILVSLSACSSIKTKSEDNFHISSQQHEKAIKSY
                     FDEAQTQGVIIIKEGKNLSTYGNALARANKEYVPASTFKMLNALIGLENHKATTNEIFK
                     WDGKKRTYPMWEKDMTLGEAMALSAVPVYQELARRTGLELMQKEVKRVNFGNTNIGTQV
                     DNFWLVGPLKITPVQEVNFADDLAHNRLPFKLETQEEVKKMLLIKEVNGSKIYAKSGWR
                     MDVTPQVGWLTGWVEQANGKKIPFSLNLEMKEGMSGSIRNEITYKSLENLGII"
                     /name="OXA-24 family carbapenem-hydrolyzing class D
                     beta-lactamase OXA-653"
     gene            complement(6005..6763)
                     /locus_tag="CDG68_RS02910"
                     /old_locus_tag="CDG68_02915"
     CDS             complement(6005..6763)
                     /locus_tag="CDG68_RS02910"
                     /old_locus_tag="CDG68_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017393773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_015060804.1"
                     /translation="MALDKSKKRGRPAQLLQIAELHAFVDYLSQKKDRSDLQNDVIAML
                     RIEKFNFESLSEAEQILVKEALKPYREHMKLNLLFDEVSVKYPQTAYERKFVQLFEAYR
                     DNALSGADFNILKTMATRYLSFKAHKLELSDLELYLSQIQKKEASKKRTAENHRKFELG
                     GAVLAAFKELGIDISQDTPEQMKNRIQNTKKFHDDVMKSKIYQEVKSYKNGYFERNKLF
                     HQVLEGLNTWKKDGELLSVIEIKKALVKNQ"
                     /name="hypothetical protein"
     gene            7067..8236
                     /locus_tag="CDG68_RS02915"
                     /old_locus_tag="CDG68_02920"
     CDS             7067..8236
                     /locus_tag="CDG68_RS02915"
                     /old_locus_tag="CDG68_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002011489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_012846858.1"
                     /translation="MASYHFSVKSKNKGYALSHYLYISRLMQYEGIRKKSAEVLEHVEP
                     GQCMPSWVKDPVEFWQAADTFERANAKAYMEYEIALPNEFTVEQRKTLVETFVDKTIAT
                     HHYPHSYAIHNVKSRLSGEEQPHCHLMFSLKADDGIERSPEQYFKRYNAKEPSKGGAKK
                     IQLQDGHADYTTYLLHIRKAWENHLNDALAEHSPTVTYQIDGQKIQIKNQVSADNYEKY
                     NEIHGTLYLPEPKLGTGKQNETVEYLAEIQKIRQHNAKERELEQRQQYFDQQHEYYYSC
                     SNEPFSSPEVMHYLNHLIKKASNKKELGHDIDEIIQTQQPILHHEKWDIDTINFNLNNA
                     RLYPEPDMEFKVRSVQPKPVIEPTSPKSKPEIQPSQPEPKRNDYDGPGF"
                     /name="MobA/MobL family protein"
     ARG             5110..5933
                     /name="blaOXA-653"
                     /gene="blaOXA-653"
                     /gene_name="OXA-24 family carbapenem-hydrolyzing class D
                     beta-lactamase OXA-653"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          2..888
                     /name="rep_cluster_1364"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          7146..8233
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ttggataaga atcaaattgt taaatcaaat caagttatag aagcttccta tcaattaagt
       61 gctgtagaac agcgtatcgt cttggcagct atttcacgta ttccgaagag ccagcctatt
      121 actgatgatg agttataccc tgtaagcgtt aatgaactac agttattagg agtacatgaa
      181 aaaaccgcat acagagactt aaaagaagga attaatagac tttatgaaag atctattaat
      241 cttagtattg atgataaatc tataaaaatg aggtgggtac aagagatcca gtttttggat
      301 agtcaaagta tcattggtat tcgtttttca aaacctatct tgcccttcat atctaattta
      361 agtagagaat ttactaagta tgctctatca gatattgctg ggatcaatag tggatatggt
      421 attcgtattt atgaattatt ggtgcaatac agacaaatag gtaagcgtga aatctctgtt
      481 gagaacttac gaagcatgtt ggagcttggc aaaaaatacc cactatttgc agatttcaaa
      541 aaacgggtaa ttgatactgc tgtagaccaa ataaatgaat gcagcccatt aagcgtttcc
      601 tatgagcaaa aaaaaactgg tcgtaaagtc actcatattc agttttcatt taaagaaaag
      661 tccaaaagca ttaatcatct caatgagcca gagaagtttt ataaattaac tgatgctcaa
      721 gtccatatgt ttggcaatca actttctcgt ctacatgaac tatctcattt agcgactcaa
      781 ggagaaagtt acgatgatct agctgcaaca atcaaagaaa tgctaagaga tccaatacaa
      841 caaaagcaat ttattcctca tcttaggaat ttaggtttta aaggatgatg tttgattgtg
      901 tatgtagcgg ttgactgttt cttgatgtat cttagttcac ataatagaca acctaaatta
      961 caacatgaaa tcactatctg ttatcgagct ttcaaacctc tatggaatga ctcgtcaagc
     1021 cgtttataaa caaataaata aaggtaattt aagtaagaat agtgatggta aaattgatct
     1081 ttctgaggcc attcgtgtct ttggtgagcc atctaaatat gtaaacagtt tacagacaac
     1141 cgaaacacga aagttgtcag aagttcactt acttgaacag caggttcaca tgcttcaaaa
     1201 acagcttgaa caagctcatg agcgtgagaa gtttcaacga gatgaattaa aagctaagaa
     1261 tgatcagtta catgttaagg atgaacaaat tgaggctatg caacgcttat tagaagcccc
     1321 aaaaaccaat atgactacgt ttgccgatca gaaacctagc caggatatag caacggatcc
     1381 tcgaccagaa ccagagccga aacatgacgg attgactact ccagttgaaa tagaaaataa
     1441 gcggattcca gtgccggagc aggttgaact ggagcagccg aaaagagggt ggttaagccg
     1501 gtttttctta cctaatgggt aaaaggcttg tttaggtgta ctaatcaaag ttgtgaaaca
     1561 ttactcaaat aataggcgtg gaacgtgaat aatcatgaaa aagccgttct agtgcgctga
     1621 actctagaac ggctttgact aaactattga tgcttataag aaaatttcaa gcatttcgca
     1681 taaggcgtat tatgttaatt ttagaaaacc ttaaatctat tttttcttta attcctctaa
     1741 aatttggtct tgtatcttac ataatttatc ttggtattct aaaagtaaat ctaatttctc
     1801 agactctaaa ttaattaatt cgctttgtaa ctctatattg tcaagcatca aattatcgta
     1861 ttcaaaacta ttcctgagtc tttctactat ttctgctgta atagaattac cagtaagact
     1921 agctgactgt tctatttctt cttttaaatc aagaggaagt ctaaaattaa cctgggtata
     1981 ctttttaatc atgaaattct agagtctaaa atgtcttgaa taactatagc acatttgcta
     2041 tatattatta tagcaaatgt gctataacat ttggagtagc tatatatgtc caacattatt
     2101 gaatttaaaa gaaatgaaag gagatttatt aatcaaaaac ctatcagtcc tgtagaacta
     2161 gtgacaaata tactcactcc acaagaggta gatataaagt ctttgattaa ggaaacaaag
     2221 aaaattttaa gagaaaaagg cttaaaattt cgtacttctc atcaagattt ggaacactta
     2281 aaaggaaata atttaatatt attttccaaa aagaaatgcg ggtttatctt aaccacaaag
     2341 ttagcctgta gcacaaatag ctcatgggaa atatattcca taagtataga taagacgacg
     2401 aaagcattag atattgttaa taaaattata tctaggctag gagataacgt tcatggcagc
     2461 agcagagaag aagcaatcag ctaatccagc gatggaagca gcgaaagagg cagcaaatat
     2521 taaaattgat ggctttgagc aaggtgtaaa tacctacaat gcgattggtt cacaattatt
     2581 gcgagaaaag cgagatccaa gtgtaattaa taaaactcgt gaaaatagac gaaatattcg
     2641 aaaagcacga gaagctatca agaatttata gtaaaaagct tcttaagatc taaaatataa
     2701 aaaattctca ttaaacaagc cctaagaaat tctcagggct ttgttcatga taaaaattat
     2761 cagaacatgt cttctgcaaa agtgactaaa acaaggctgt gaaactccaa ttccgtatat
     2821 acacaagcat ctagtctaag aaatttttcc ttatcagatg gaaaattata aggtacagac
     2881 caaggtttta ttaaacaagg acttacctta tcaaaagttc caataaattc ctttttagtt
     2941 aaatcataaa ttctgattac acttaactta acaatactca aaacccaaag catgtggccc
     3001 ttataggtct gataattatc acaaaccagc acaccatact tttttaataa gtatttgtat
     3061 ataaatgagg aaactccatt tcgctgataa ttggctaaaa tcgatgcacc ttttatttgt
     3121 ttacctgtta ttttaataga gtcattaata atttcgatat ctttaaatgc gtatctcgct
     3181 atcaaaattt ctttctctac ttcataagct tctaaggctt tattagcagc gtcatattca
     3241 tctaaatatt catcagcaac agcacaaacc ccatccaatg catactcatt attaaattca
     3301 caataaaaaa gctcaataca tccagaatac tcgctacctt ctgaagcttg caattctttt
     3361 aaacaaaata ttccaccttc cctttggttg tagaagtact tactttcaag aatattttca
     3421 taagaaaagt aaacaggtag tggaattaaa ggcttattat aattttcttc gagaagtatg
     3481 tgtggaggag tcaatttaaa ccttatattt catcaagcta ctaatgttgg tttaataata
     3541 tcattaattt tagcttctaa cattttctca taagaagtta attctggata ctgtttggat
     3601 gctttaatag aaaatcttaa gctattattt ctttcttgaa tttcagctac aactttcaca
     3661 tcatcccaat cttttttatt gagaataata tctttgaaaa taagtatata ttgcttattt
     3721 ttttctttct cagaaaaacg tcttagttca aatgtttgac gaatattagg catagtaaaa
     3781 cgctgaacta aatcagtact atttttgagg gtttgaacag ttctagtgaa cattttgaca
     3841 cctacatacc taaagaaata aaagaaaata aaaatatata gtgacaggtt ataagacaaa
     3901 tatgtcacta tcaactacgt caaatttggc gtactatata tatattaatt tatatatata
     3961 tatgaatagg gttttaaaaa attatgttaa attttgaatt aaaagaaaag tggggcgaaa
     4021 actctctaat attagggttc acgcagatcc caactactct aatttatgca caaaaagagc
     4081 ttggtctatc atctatagaa attaacatct tacttaactt attgacccat tggtggaaga
     4141 aagaagaatt tccttatcca tctcaagctg gaattgcata tcgaatggga gtttctacta
     4201 gaactgtaca acgaacatta gcaggtcttg aaacaaaagg attcataacc agaaacaaaa
     4261 catcaagaga taacagtaaa tacaaaggaa ggagtattta tgatttatct ccattagtaa
     4321 aaattctgga agaaaaggca cctgatctag atattgtgaa aaaaattaaa aaaaataaaa
     4381 gactagctaa atagatttaa cataaaatct cttacgataa gtcaaaaaag ggagcttaaa
     4441 gctccctttt tctatctaac tatatgaatc ttcggtgatg tttgtatacc taattgcctg
     4501 aaaatatgcc atagagctat gttgcgatac tggtacatag caatttgtga aaaatcaaac
     4561 cattgttcgg atgtgaattg gtttatatca gaaaaaggtg gataacctct tagagctaga
     4621 aatgcattaa tttcttctac cattggatcg agctcaaaca tgttttaaaa cctaataaag
     4681 aataaagtcc aattgtaaat cccctttaaa aggtcacttt tttattgttc caaatcctct
     4741 ttacagtttt tttgttctct actttacaat tgacttggga aattatcccg atgtttgtcc
     4801 gcaggaaatg gggataaaag tgggttattg gagcattgag aaatcaggaa aaattaaatt
     4861 tttgagtatt ttttaatcga ttttgttggc taaaagtgac aaagttgctt aaaaagcatc
     4921 cgacttgttt ccaagtcggc ttttcaccta ttttttagtc atgactaatt ctataaataa
     4981 ttaaaaaata ataaattttc ataatttatt tcgcataacg cccattatgt taaattaaaa
     5041 gagttttaac ttttttaata aaagtattat gctctaagcc ccaaaatttc ccctaacatg
     5101 aatttgtaat gaaaaaattt atacttccta tattcagcat ttctattcta gtttctctca
     5161 gtgcatgttc atctattaaa actaaatctg aagataattt tcatatttct tctcagcaac
     5221 atgaaaaagc tattaaaagc tattttgatg aagctcaaac acagggtgta attattatta
     5281 aagagggtaa aaatcttagc acctatggta atgctcttgc acgagcaaat aaagaatatg
     5341 tccctgcatc aacatttaag atgctaaatg ctttaatcgg gctagaaaat cataaagcaa
     5401 caacaaatga gattttcaaa tgggatggta aaaaaagaac ttatcctatg tgggagaaag
     5461 atatgacttt aggtgaggca atggcattgt cagcagttcc agtatatcaa gagcttgcaa
     5521 gacggactgg cctagagcta atgcagaaag aagtaaagcg ggttaatttt ggaaatacaa
     5581 atattggaac acaggtcgat aatttttggt tagttggccc ccttaaaatt acaccagtac
     5641 aagaagttaa ttttgccgat gaccttgcac ataaccgatt accttttaaa ttagaaactc
     5701 aagaagaagt taaaaaaatg cttctaatta aagaagtaaa tggtagtaag atttatgcaa
     5761 aaagtggatg gagaatggat gttactccac aggtaggttg gttgactggt tgggtggagc
     5821 aagctaatgg aaaaaaaatc cccttttcgc tcaacttaga aatgaaagaa ggaatgtctg
     5881 gttctattcg taatgaaatt acttataagt cgctagaaaa tcttggaatc atttaataat
     5941 ttctaaaatt aacataatac gccttatgcg aaatttgcct gaatagcctt tctcagaaag
     6001 gctattactg atttttcact aaagctttct taatttcgat gactgacaat aactctccgt
     6061 cttttttcca ggtattcagt ccctctaata cctgatgaaa aagcttattt ctttcaaaat
     6121 atccattttt ataactttta acttcttgat atatcttaga tttcattaca tcatcatgaa
     6181 attttttcgt attctgaatg cgattcttca tctgttcagg cgtatcttga ctaatatcaa
     6241 tccctaactc cttgaaagca gctaacacag caccgcccaa ctcaaactta cgatgatttt
     6301 cagccgttct ttttttgctg gcttccttct tctggatctg actcaaataa agctctaaat
     6361 cactcaactc aagcttatgc gctttgaaac tcagataacg agtcgccatg gttttcaaga
     6421 tattaaaatc agcaccactc aacgcattgt cccgataagc ctcaaacaac tgcacaaact
     6481 tccgttcata tgcggtctgc ggatacttca cagacacctc atcaaacaat aagttcagct
     6541 tcatatgctc acgataaggt tttaaggcct ctttcaccaa gatctgttca gcttcagaca
     6601 atgactcaaa gttaaatttt tcaattcgta gcattgcaat cacatcattc tgcaaatcag
     6661 aacgatcctt cttctgtgaa agatagtcta caaatgcatg taactctgcg atttgtaaaa
     6721 gttgtgcagg tctgccacgt ttttttgatt tatccaatgc catctatctg ccgcctaatt
     6781 ttcagaactg ctaccgattg agttatggtt ttaccataat tcaatcgggc agtctgaaaa
     6841 taattattta aaataaaaat aaaatttctt gaaaagaatt tatattttta ttttaaataa
     6901 ttcgcacttg acagtttctc ctaatcggag aaactagtac gcaatggggc gattccccct
     6961 tgaccccctt taaccggatt atttcagccc tgtaggcatt acataatccg gagattttta
     7021 ttgcgtttca ctccataaaa accaacaaca ccccaaggaa gtgaacatgg ccagttacca
     7081 tttcagcgtc aaaagtaaaa ataaaggcta cgcactgagc cactacctgt acatttcccg
     7141 actcatgcaa tacgaaggca tccgcaaaaa gagtgcagaa gtcctcgaac atgtcgaacc
     7201 agggcaatgc atgccctcct gggtcaaaga tccggttgaa ttctggcaag cagcggacac
     7261 ctttgaacgg gccaatgcca aggcttacat ggaatacgaa attgccctac cgaatgaatt
     7321 caccgtagaa caacgtaaaa cgctcgtaga gacatttgtt gataaaacca tagcaacaca
     7381 ccactaccct cattcctacg ccatacacaa cgtaaaatca cgcctcagtg gtgaagaaca
     7441 accccattgc cacctcatgt tttcactcaa agccgatgac ggcatagaac gaagtccgga
     7501 acaatacttc aaacgctaca acgctaaaga accaagcaaa ggcggagcaa agaaaattca
     7561 actacaggat ggccatgccg actacaccac ttacctccta catatccgaa aagcctggga
     7621 aaatcacctg aatgatgcat tggcagaaca tagcccaacc gtgacttacc agatcgatgg
     7681 ccagaagatc caaattaaaa accaagtttc ggcagacaac tacgaaaaat acaacgagat
     7741 acacggcacc ctctacctac cagaacccaa actggggaca ggcaaacaaa atgaaaccgt
     7801 ggaataccta gcggaaatcc agaaaatcag acaacacaat gccaaagaac gagaactgga
     7861 acagcgccaa caatactttg atcaacagca tgaatactac tacagctgct caaacgaacc
     7921 cttcagttct ccggaagtga tgcactacct caatcacctc attaaaaaag ccagtaacaa
     7981 aaaagaactg ggccacgata tcgacgaaat tattcaaact cagcaaccaa tcctgcatca
     8041 cgaaaaatgg gatatcgata ccatcaattt taatctcaat aacgccaggt tatacccaga
     8101 accggatatg gagtttaaag tccgatccgt acaaccaaaa ccagtgattg aaccaacatc
     8161 accgaagtca aaacctgaaa tacaaccctc acagcctgaa ccgaaacgaa atgattatga
     8221 tggtccgggt ttttaaaaat ggatctagat cagaaagtac aaagcgagtg tctacgagcg
     8281 acatcaccaa aattagcgcc tcgactttct gaaaaaccgc tttttaaggc tgtatagcct
     8341 aaaaaagcat aaatcagatg ctaaaactgg cttccagacc gagcgaagcg agcaaaaagc
     8401 tttatgagcg aagcgaattc cgagttgctt ttgctcttgc ctttactttt tctcaaattc
     8461 acgaaggaat taacagaaaa attgccccct gaatcgagcg aaagcgagat tcaatagagt
     8521 ttgagcgtag cgaaaaccac gggcaatttt ttcaaacgtc ttttttaatt atttttatat
     8581 cttttaaatc ttttctttga gctgtatccc ttatggagca agcctttcaa agtttaacta
     8641 tgacggattg actactaact atgacggatt gactactaac tatgacggat tgactactta
     8701 actatgacgg attgactact aactatgaca agtatatagt tgtcatatat aaaagaaaat
     8761 caatggtttt att
//
