LOCUS       NZ_CP033126            12655 bp    DNA     circular CON 05-JAN-2024
DEFINITION  Acinetobacter wuhouensis strain WCHAW010062 plasmid p6_010062,
            complete sequence.
ACCESSION   NZ_CP033126
VERSION     NZ_CP033126.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN07138954
            Assembly: GCF_002165345.2
KEYWORDS    RefSeq.
SOURCE      Acinetobacter wuhouensis
  ORGANISM  Acinetobacter wuhouensis
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter.
REFERENCE   1  (bases 1 to 12655)
  AUTHORS   Hu,Y., Long,H., Feng,Y. and Zong,Z.
  TITLE     The complete genome of Acinetobacter wuhouensis strain WCHAW010062
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12655)
  AUTHORS   Hu,Y., Long,H., Feng,Y. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-OCT-2018) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
REFERENCE   3  (bases 1 to 12655)
  AUTHORS   Hu,Y., Feng,Y. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2017) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/04/2024 23:19:32
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,443
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 4,234
            CDSs (with protein)               :: 4,234
            Genes (RNA)                       :: 107
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 27 of 102
            Pseudo Genes (incomplete)         :: 73 of 102
            Pseudo Genes (internal stop)      :: 16 of 102
            Pseudo Genes (multiple problems)  :: 14 of 102
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP033126.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..12655
                     /organism="Acinetobacter wuhouensis"
                     /mol_type="genomic DNA"
                     /strain="WCHAW010062"
                     /isolation_source="Sewage"
                     /db_xref="taxon:1879050"
                     /plasmid="p6_010062"
                     /geo_loc_name="China: Sichuan, Chengdu"
                     /collection_date="2015-10-31"
     gene            complement(join(12141..12655,1..388))
                     /locus_tag="CDG68_RS00810"
                     /old_locus_tag="CDG68_00810"
     CDS             complement(join(12141..12655,1..388))
                     /locus_tag="CDG68_RS00810"
                     /old_locus_tag="CDG68_00810"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="WP_087554478.1"
                     /translation="MNSYPLKDLTELSEELLVEYSFVEKDWFITKVLTLLADDPNLVFS
                     GGTSLFKAHRLIERFSEDIDFLVIGDFKPAKRKEIRNRIIQTLIENGFNVIDKKTFDSG
                     RKYELTIEYQITQATPDYIRPHILIEICFKESQDFEPVYRDAGSLLHIYSQRELEIKRI
                     TCTDPRQIFADKISALIWRIPDCNRTIADSDSQKLDNTIIRHLYDICKLAEALSIDSPE
                     LITLIESKISNDMTRDRSGTEITISAQEYGLRTIATLKDSTLWKDSYHSYVSNFCLKGN
                     IIAYEQAISIIENIIKKFK"
                     /name="nucleotidyl transferase AbiEii/AbiGii toxin family
                     protein"
     gene            complement(394..789)
                     /locus_tag="CDG68_RS00815"
                     /old_locus_tag="CDG68_00815"
     CDS             complement(394..789)
                     /locus_tag="CDG68_RS00815"
                     /old_locus_tag="CDG68_00815"
                     /inference="COORDINATES: protein motif:HMM:NF040319.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6088 family protein"
                     /protein_id="WP_087554476.1"
                     /translation="MNIATRMKISVANSRNNVFLSKDFMKMGSESQVRMGLQELICEKR
                     VLRISKGVYVRAKESRLTGKVVPVNGLETVKEAINRLGYQIKESQSIKNYNSKISTQVP
                     TGRTIAINKCSKRKFRYDNIEIFLVYY"
                     /name="DUF6088 family protein"
     gene            937..1800
                     /locus_tag="CDG68_RS00820"
                     /old_locus_tag="CDG68_00820"
     CDS             937..1800
                     /locus_tag="CDG68_RS00820"
                     /old_locus_tag="CDG68_00820"
                     /inference="COORDINATES: protein motif:HMM:NF039696.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6236 family protein"
                     /protein_id="WP_087554474.1"
                     /translation="MKKGILITSPKEIAIGGGISFSSLDRRDLRKALTYWDEIAYLTNN
                     LVYQSDDELDKLKEFGAASELNTPLIGSIRGNQEFIKFIKLSEINTINSKLKENPAYWS
                     VANNSYLDRNEEYLANDKELSLEIELYQTLPVPSADTPLEKIIEFKEKRGTELIAFREY
                     FEGLVIDICDSNSYDRKKNLEIERVKRSLSDIDRVMREGKIKIIYDNLRNIVKKDFLDI
                     SAYTATTSGLTAYGVYSTPQLHAFSPMLAGIAACGIAIGYKYINKPQTHTQFNYIRSIR
                     KNFQDS"
                     /name="DUF6236 family protein"
     gene            complement(2374..3015)
                     /locus_tag="CDG68_RS00830"
                     /old_locus_tag="CDG68_00830"
     CDS             complement(2374..3015)
                     /locus_tag="CDG68_RS00830"
                     /old_locus_tag="CDG68_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016927662.1"
                     /GO_process="GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="WP_004787586.1"
                     /translation="MSKKDIAPQRLTREIVLRTALDMLNEEGIDSITTRKLAQRLGIKS
                     PTLYWHFKNKSLLMEAMAETIINEHHLVSLPIDGMTWQDWLLANSVSFRRALLAYRDGA
                     RLHARTSPSQGHFNTIEAQVALLSHAGFSPVEAVALLMTLGRFIVGWVLEEQQEEIRSD
                     PPFEADPTIYPLMLQGVNTLQNMNADDIFENGIRMVIIGAERQLDIKMQT"
                     /name="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
     ARG             3148..4268
                     /name="tet(39)"
                     /gene="tet(39)"
                     /gene_name="major facilitator superfamily (MFS) antibiotic
                     efflux pump"
                     /tool="rgi"
                     /drug_class="tetracycline antibiotic"
                     /identity="100.0"
                     /coverage="94.43"
     ARG             3082..4265
                     /name="tet(39)"
                     /gene="tet(39)"
                     /gene_name="tetracycline efflux MFS transporter Tet(39)"
                     /tool="amrfinderplus"
                     /drug_class="TETRACYCLINE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            complement(4497..5042)
                     /locus_tag="CDG68_RS00840"
                     /old_locus_tag="CDG68_00840"
     CDS             complement(4497..5042)
                     /locus_tag="CDG68_RS00840"
                     /old_locus_tag="CDG68_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017398013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication DNA-binding protein"
                     /protein_id="WP_171422345.1"
                     /translation="MKALSVIELAQLYGMNRQSIYKRINKGDLSKNSDGKIDLSEAIRV
                     FGEPSNKNNNVTTLQSTTVQKDTEVDMLKQQVDILKKQLELAHEREVFQREQLEAKDNQ
                     IEAIQRLLEAPKTNMTTFTDQKSSQDIATDPRSEPEPKHDGLTTPEKTENKRIPVPERI
                     EQEPEKRGFWSRFFLPNG"
                     /name="plasmid replication DNA-binding protein"
     gene            complement(5111..6040)
                     /gene="repM"
                     /locus_tag="CDG68_RS00845"
                     /old_locus_tag="CDG68_00845"
     CDS             complement(5111..6040)
                     /gene="repM"
                     /locus_tag="CDG68_RS00845"
                     /old_locus_tag="CDG68_00845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237331.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepM"
                     /protein_id="WP_087554487.1"
                     /translation="MSKELVVKTNRLNQAFQTLSLSEFHIVQLAIVDARHTGTGLSTDT
                     PLRIDALRYAEVFGTTRQNAYQRMKEAEDSLFNRRFSFFDEDGKLVKSRWIQQVKYLDD
                     EGAIELVFTLAVVQGISKIDGIKEFFTQYLLSQTAQLNSTYSARLYELLIQWKAIGKTP
                     VFELATFREQLGIGVNEYKRMDHFKTRVLDLAISEISEKTDIEATYQQHKKGRSISGFS
                     FSFKQKKSKTKSLENQTISGNLDLFSKKMTDSQRHLFSNKLSELPEMSKYSQGTESYPQ
                     FAVRIAEMLKDSEKLKEFAPMLEKVGYR"
                     /name="repM"
     gene            complement(6746..6952)
                     /locus_tag="CDG68_RS22410"
     CDS             complement(6746..6952)
                     /locus_tag="CDG68_RS22410"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_133356867.1"
                     /translation="MVLESRSCGTKTQPKFVLNLVVMTVGHKLSERFEIRFLCYAKRMS
                     QVDNCENVPIRSIGVRRLRETPT"
                     /name="hypothetical protein"
     gene            8678..9802
                     /locus_tag="CDG68_RS00855"
                     /old_locus_tag="CDG68_00855"
     CDS             8678..9802
                     /locus_tag="CDG68_RS00855"
                     /old_locus_tag="CDG68_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004728239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_087554489.1"
                     /translation="MQCVSNGKAKFWFSDVVPFSKFQNLIPKLILEYGLDLGEQARKKK
                     SDYGEPVWSLVINYDPAKSEVFQFWLFTTGYREARRSKLTLKEILAKNSSMARKQKLNS
                     ILTTKKEKLLRFGPYVLGQYIEFSDLKTEFAKIYYFPENYGVVFNTHTKRVKAIDSNKK
                     FIYRIFKPFDASEERRFKSLYRKFAFVFLDNEHNRWNQESVTQFLLGFGIKFENDESYN
                     DRLKELTRVLRLVRKKHLEFFQRYSTKRVRFTWYLSQDFLDGSKREFEKRLNKIQTTPE
                     SIEKFAEASARLMAHGNFHGTRFQIGSIQARVRKLLNQIQPDHKKLNKKYFTNHLHYVR
                     FTKKKAMSIKEFEMACRNADTMYLNKQNKQKLKD"
                     /name="hypothetical protein"
     gene            9819..10112
                     /locus_tag="CDG68_RS00860"
                     /old_locus_tag="CDG68_00860"
     CDS             9819..10112
                     /locus_tag="CDG68_RS00860"
                     /old_locus_tag="CDG68_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016658922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin HicB"
                     /protein_id="WP_087554484.1"
                     /translation="MLKYPDDYDLSRQEIDDLRAETKDAAKYMKALFNFKIEARYSYSV
                     TWSAQDGEFVGLCAEFPSLSWLDPDSDKARSGIERLVFDTLQDMNSTGEIIP"
                     /name="antitoxin HicB"
     gene            complement(10109..10432)
                     /locus_tag="CDG68_RS00865"
                     /old_locus_tag="CDG68_00865"
     CDS             complement(10109..10432)
                     /locus_tag="CDG68_RS00865"
                     /old_locus_tag="CDG68_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004763341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_087554482.1"
                     /translation="MNHTIFQKLEKMNGHCKTCAKAYALLTLLSLVTFFIYFFNHFTLE
                     TLFTDPNALLPAIKIEAFALGIMHIVYSFFFKYIDKKLQIYGLDSNNLNEYIQRNQAFF
                     QKY"
                     /name="hypothetical protein"
     gene            10933..11196
                     /locus_tag="CDG68_RS00870"
                     /old_locus_tag="CDG68_00870"
     CDS             10933..11196
                     /locus_tag="CDG68_RS00870"
                     /old_locus_tag="CDG68_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004676203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_087554481.1"
                     /translation="MSAVNLINENDDEREIASQAACTLRESFVTAAQSGPVMYVENDTV
                     LLKELNGPPIVIKQLSGRNPELAQRVASRGTFKIKKRKVSQD"
                     /name="hypothetical protein"
     gene            complement(11304..12119)
                     /locus_tag="CDG68_RS00875"
                     /old_locus_tag="CDG68_00875"
     CDS             complement(11304..12119)
                     /locus_tag="CDG68_RS00875"
                     /old_locus_tag="CDG68_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004889734.1"
                     /GO_function="GO:0008233 - peptidase activity [Evidence
                     IEA]; GO:0008236 - serine-type peptidase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006508 - proteolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="WP_087554479.1"
                     /translation="MFKNLYEKIQRCCGYVTVFEGDEVISEGTCFSFTDTGEVITAAHV
                     VTGRMPIKKEDYTHPQQRILIKFPELPLVEYAVSFCSFEINVPSFKEIIQLDIAVLRPK
                     EEQPFKFPFLPASINSVNLGKRVFVAGYSDELNIPFSIEKIIDPESFGADKFIKAIREE
                     GYLADMTGPIIKHGYIGNIRRIFADDNSQNIRIETDVFYIDNGMHSGASGGPIVNEEGV
                     ALGVLTQRAITPAHQSEDSILKVPSGSSIGISLQILKTVHEIIQRNNAT"
                     /name="serine protease"
     Typing          complement(5112..6040)
                     /name="rep_cluster_1261"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.462"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ctattagaat atggggtctg atatagtcgg gagtagcttg agtaatttga tattcgattg
       61 ttaattcata ttttcgacca ctgtcaaaag tttttttatc aattacatta aaaccattct
      121 ctattaaagt ttgaataatt ctattgcgga tttctttacg ctttgcaggc ttaaaatcgc
      181 caataaccaa gaaatcgata tcttcagaaa agcgttcaat tagtctatga gctttaaaaa
      241 gtgaagtacc acctgaaaaa actaaattag gatcatcagc gagtaacgtc aggactttag
      301 tgatgaacca gtctttttct acaaagctat attcaacaag taattcttct gagagttcag
      361 ttagatcttt taaaggataa gaattcattt aaattaataa tatacgagga atatctctat
      421 attatcgtat ctaaactttc tttttgaaca tttattaata gcaatagttc taccagttgg
      481 aacttgtgtt gatatcttcg aattgtagtt tttaatagat tgagattctt tgatttgata
      541 gcctaaacga tttatggcct cttttacggt ttctaatccg tttacaggaa caaccttacc
      601 tgtaaggcga ctttctttag cccttacata tacaccttta ctgatcctca gaactctttt
      661 ttcacagata agttcttgca aacccattct aacttggctt tcagatccca ttttcatgaa
      721 atcttttgat aaaaagacgt tatttctact gttagccacg gaaattttca ttcttgtagc
      781 aatgttcata ttgattccta ctttacaaag aaaagatttg gcaaaagaga gactatatgt
      841 agaagtatat actaatagct aaatttaaaa aaactgttgg tgagtgacta atttaatgtc
      901 tatatttgtg agagatctta ttttgttgat aaatatatga aaaaaggtat tttgattaca
      961 tctcccaaag aaatcgcaat tggtggtgga atttctttct cttcacttga tcggcgtgac
     1021 ttgcgtaaag ctttaaccta ttgggatgag atagcatatc taacaaataa tttggtttat
     1081 caatcagatg atgagctaga taagttaaag gaatttggtg ctgcgagtga attaaatact
     1141 cctttaatag gaagcattag aggtaatcaa gaatttatca agtttataaa attatcagag
     1201 ataaatacaa taaattcaaa acttaaggaa aatcctgcat attggtctgt agcaaataat
     1261 tcttatcttg atagaaacga agagtattta gctaatgata aagaattaag cttagaaatt
     1321 gagttatatc agactttgcc tgttccatct gctgatacac ctctagaaaa aattattgag
     1381 ttcaaagaaa aaagaggaac agaattaata gcatttcgag agtactttga aggtttggtc
     1441 atcgatatat gcgattcaaa ctcctatgat aggaagaaaa acctagaaat agaaagggtt
     1501 aaaagatctt tatctgatat agatagagta atgagagaag gtaaaattaa gattatctat
     1561 gacaatttaa gaaatattgt taaaaaggac tttttagata tatctgctta tactgctaca
     1621 acatcagggc ttactgctta tggtgtctat tctacacctc aattacatgc atttagtccc
     1681 atgctggcag gtattgctgc ttgtggtata gctataggat ataagtatat taataaacca
     1741 caaactcaca cccaatttaa ttacatacga agtattcgta agaactttca ggatagttag
     1801 gtttagtttt cctaaaatta gcataacggc tgttatacca gattcgttag acaataaaaa
     1861 agcccaacat cctgttgggc ttttttacat atttgatgcg tttggtataa ctcctgtcgt
     1921 acctgcattc tccgttgctg tcttttctta ttcttatctg gagcatcctg ctctctatgt
     1981 ccccatgata ggaaattgat gaagaattgc atagacgcaa agcacctgaa tgaatgtgcg
     2041 ccaaaacgta caagtctggc cactttttat cccaagagtt gtccgcagaa attggggatt
     2101 gtttcggccg tggatggctt gataaagcac aacaatgcgt cattttgatt aaacattgat
     2161 cgattttatt ggcaagaagt atcaaacctg atcataaaaa agccgacttg tttctaagtc
     2221 ggctttttgc agttttttag gtaggtcatt ttttttaact atctgaaaaa caaaggcttt
     2281 atagatagcg tttcgtataa gctctattat gttaaataat taaggataaa agctttatta
     2341 ctaaatagaa cagttgctta aagcagtata gagttaagtt tgcattttaa tatcaagttg
     2401 tctttcagca ccgataatca ccatacgaat cccattttca aaaatgtcat ccgcattcat
     2461 attttgtaag gtattcactc cttggagcat gagtggatat atagttggat cggcttcgaa
     2521 tggtgggtct gatcttattt cttcttgctg ctcctcaagg acccacccaa caataaacct
     2581 tcctaatgtc ataagtaatg caactgcttc aacaggagaa aacccagcat gagataacag
     2641 cgcaacttgg gcctcaatcg tgttgaaatg tccttgactg gggctagttc ttgcatgtag
     2701 acgagcacca tcacggtaag ctagtagtgc tcgacgaaag cttacagaat tagcgagcaa
     2761 ccaatcttgc catgtcatcc catcgatggg taaagaaact aaatggtgtt catttataat
     2821 ggtttccgcc attgcttcca ttaacaagga tttattcttg aaatgccaat atagagttgg
     2881 agatttaata cccaatcgtt gagctagctt gcgtgtggtt attgaatcta tgccttcttc
     2941 attcaacata tccaatgcgg tacgcaggac tatttcacgt gtcagacgtt gaggtgcaat
     3001 atctttttta ctcataaatc tatcactgat agggagtgtg tgagagaata ttaattctat
     3061 cattgctaga gaaggcattt gtgaagaaat cattgagcgt gattttaatc actatatttc
     3121 tggatgctgt tgggattggt ttaattatgc cgatcttgcc tgaattatta cggtcattgg
     3181 ctggagctga agcaggcggt gttcactatg gtgctttatt agctgtgtat gctctgatgc
     3241 agttcatttt tgcacctatc cttggagcgt tgagtgaccg atttggacgt cgacctgtat
     3301 taattatttc aattgctggt gcaacggctg attatctcct aatggctgct gctccttctc
     3361 tattgtggct atatattggt cgtatttttg cgggaattac aggtgccaac atggctgttg
     3421 caacagctta tgtttcagat attactccag cccatgagcg tgcaaaaagg tttggtctcc
     3481 ttggagctgt ctttggtatt gggtttatag cgggtccggt aataggtgga gttttgggtg
     3541 aatggaactt acatgcaccg ttctttgctg ctgcttttat gaatgggatt aatttaataa
     3601 tgacagcagt cttattaaaa gaatcaaaac acagcaataa aatgactgag aaggttcagg
     3661 agcaatcaat attaaagaaa ttatcctatt tgatcactca acctaatatg gctccattgc
     3721 ttggtatctt tttaattatc acattggttt cacaagtccc cgcaacttta tgggttatct
     3781 atgggcagga tcgttatggc tggagtatat ttattgcagg tgtttccctt gctagttatg
     3841 gaatatgcca ttctattgca caggcttttg ctatcgcccc tatggtaaag aggtttggag
     3901 agaaaaatac gttgttatgt ggaatagctt gcgatgcaat tggtttactt cttttatcta
     3961 ttgctgttga agaatgggtg ccttttgcgt tgttaccatt gtttgccctt ggtggagtag
     4021 ccgttcctgc tttgcaagca atgatgtcca gaggtattag tgatgaaaga caaggtgaat
     4081 tacaagggct attaagcagt tttaatagtc tgggggctat aattggtcct gtattagtta
     4141 ctagcctcta ttttatgact caggcatcag ctcctggaat ggtatgggca ttagctgcaa
     4201 tactttatgt aatcacccta cccttattgc ttaagtatcg cctgaataaa tattctggag
     4261 ttccataaaa tatttaattt attggtttaa aaactaatgg attctatagt ttatctaaaa
     4321 ttaacataat acaccttata cgaaatgcaa ttttttattt cttataattc ataatcttat
     4381 gtcgatgtga aaagcccaac ttagctgatt ggggcttttt tgttgatttt tcatgttcca
     4441 cgcttaactt ttgagcaatg ttccacgatt ttaataagat caggtgaaca atcccctcag
     4501 ccattcggca gaaaaaaacg gctccaaaag ccccttttct ccggttcctg ttcaatgcgc
     4561 tccggtactg gaatccgctt attttccgtt ttttccggag tagtcaatcc gtcatgtttc
     4621 ggctcaggtt ctgaccgagg atccgttgct atatcttggc tagatttctg atcggtaaac
     4681 gtagtcatat tggtttttgg ggcttctagc agtcgttgta ttgcttctat ttgattatct
     4741 tttgcttcta gttgttctct ttgaaaaact tctcgctcat gtgcaagttc tagctgtttt
     4801 ttaagtatgt ctacttgctg ttttagcatg tctacttctg tatctttttg taccgtagta
     4861 gactgtaacg tagttacatt attattttta ttagatggtt cgccaaatac tcgtatggct
     4921 tcagataaat ctattttccc atcactattt tttgataaat cccctttatt tatacgttta
     4981 tagatacttt gacgattcat tccatagagt tgagctaatt caataacaga tagtgctttc
     5041 atcatgtaac ccaagtaaaa gtctgtatgt cagtagacaa tattcagtga tgtaacgtta
     5101 catcaatgat ttaacgatag ccaacctttt caagcatggg agcaaactct tttaacttct
     5161 cagaatcttt aagcatttca gcaattctta ctgcgaattg aggatagctt tcagttcctt
     5221 gagagtattt actcatttca ggaagttcag atagcttatt ggaaaataag tggcgttgag
     5281 agtcagtcat tttttttgaa aaaagatcta gattcccaga tattgtctga ttttctaatg
     5341 atttagtttt agattttttt tgtttaaaag aaaaagaaaa accagatatt gaacggcctt
     5401 ttttatgctg ttggtaggtt gcctctatat ctgttttttc gctaatttca gaaatggcta
     5461 agtcaagaac tctagtttta aagtgatcca tacgcttgta ttcattaaca ccaattccaa
     5521 gttgttctct aaaagttgct aattcaaata ctggcgtttt tccaatagct ttccattgaa
     5581 ttaataactc gtataatctt gctgaatatg tactgttaag ctgggctgtt tggcttagta
     5641 aatattgagt aaaaaattct ttaataccat caattttact tatgccttgt acaacagcta
     5701 aagtgaaaac aagttcaata gccccttcat catctagata ttttacttgc tgaatccatc
     5761 tacttttgac taatttccca tcttcatcaa agaagctaaa tcttctgtta aataaagaat
     5821 cctctgcttc tttcattctc tgataagcat tttgccttgt tgtcccaaaa acttcagcat
     5881 atctcaaagc atctattcgt aaaggagtat ctgtacttag tccagtaccc gtatgtctag
     5941 catcaacaat ggctaattga acaatatgaa actctgataa agatagggtt tgaaaagctt
     6001 ggtttaaccg attggttttt acaactaatt cttttgacat agactcaatt gaaatatttt
     6061 tatacccaca gattataaca atgtgggtat aactgcaact gtgtagttaa gggtgtaatt
     6121 aggagggtaa ctaaacgtgg gggaatgggt aactaaacgt gggggaatgg gtaactaaac
     6181 gtgggggaat gggtaactaa acgtggggga atgggtaact aaacgtgggg gaatgcaaag
     6241 gttagttatt gaaatttaat aattaaaact aagttaaaaa catttaaaaa ctttaaaaat
     6301 aattaaaagc ccagtgcatg aaaaattgcc cttggttttc gctacgctca aactctattg
     6361 aatctcgctt tcgctcgatt cggggggcaa ttttttggtc aatgtttccg tgacttttag
     6421 aaaaagcaaa agcaactcgg aattcgcttc gctcataatt cttttttagt cgcttcgctc
     6481 ggtctagatt caatttttta tatgtaatct aagtttttcc caggatctaa aactgtaaaa
     6541 aacggttttt cagagagtct aggcgcaact tttgagcgtt cgctcgtaga cactcgctct
     6601 atagataccg attaaaggct tatattggcg tttttagctt gattgcagct ttttatgata
     6661 ttgggtagct ttttttataa aaagtgtctt aaaacgcaaa ttagcgcaaa ataggaaagc
     6721 cctgtctact gatgcagaac agggcttatg ttggagtttc acgtaaccgc ctaacgccaa
     6781 tagaacgtat tggtacgttt tcacagttat caacttgtga cattctcttt gcataacaaa
     6841 ggaagcgaat ctcaaaccgt tcagacagct tatgcccgac tgtcatcacc actaagttta
     6901 agacaaactt cggttgtgtt ttagttccac aactacgact ttctaatacc acttttccgc
     6961 tagttacgaa gcaatatgtt ttccatcacc gctaagtaca ggtccgctaa tattcaacca
     7021 tcaaattcaa tcgtcaccgc taagtcacat ttggaccgct agatgaaaat tcaggaaaga
     7081 atacatcacc gctaaaacac tattccgcta gtaaaacaac ttgactgctt cataacgtca
     7141 ccgctaaaaa aaatagctag tagcgattaa ttcaacttta aagtttggat taataactaa
     7201 gatatttggt tggcgtgaac caacaacgga gccaccgttc atgcttctta ctgaataatc
     7261 atcaaatatc acaactacta acagccacgt taataattga aataatcatt ttgatgttct
     7321 tccacgccaa aaaagttcgt accatttcat agataccgag gctgccacac ctcactttaa
     7381 aaacccttgt cagtggtttt aaatatttcc cgcattgtct ttcgcttcat caccgcttac
     7441 gtttttattc gctcgaagtg actggatctg tatcaaggtt cgatacagaa tctactcaac
     7501 aattctccct ctctcattaa tcaaaactta ttccccactc aaggcaggtt ttcgcttcaa
     7561 aagtctatat caaggagcca ccttgtataa tgatcgactt gagagattcc catattagtc
     7621 ataatggtct ttgttcatta ataaaagtaa accagtatta ataaacgatc agtacgctta
     7681 tccactccac aaaataacag cgattccgtt gttctacggt aagagttcca aaaacccgta
     7741 ttattcaccg ttattcgact tcattgtggg taaagcactt gacctacgca aagcaacatc
     7801 ttcgctatgt ttaaatcact aaaaaataaa aaagaccgca atgaacttaa tcaatgcagt
     7861 cttaatttct gagtgacata acgttctacg tgtcaccgtt acaggcttga gctatccagc
     7921 gatctcacca actaacctga aatgtcatct cgacaagtca ttcaagttaa tccttgagta
     7981 ccgaagtttc cagatttctc ctcaactccc taatacctcg cgatattctc agcagcccac
     8041 ctgtgcgcca tacatcatgc ccttcgtaaa aattgaagca tgaaataggt gttgttaacg
     8101 caactatcaa tccttgaagt tactcacact ctccattaga cagggtttga tctagtcgcc
     8161 tagattcccg tttctatgtc gcccaactat tacatagatt acatatttac gactggtgag
     8221 cttaaaagca gacctgcgtt tatatgcagt caggattggg tcaataagcg gaatttcacc
     8281 acctagtgcc cgtcatagcc gcgccagcta gatttctcac gcacgcagct ttgcgagttt
     8341 tagtatacgt tggctgacgt atacacataa aaccgcaatg acaagggtct agaaaaattc
     8401 gggacagata cgaatcacat cgatcccaaa aaattcatat ttggcaggca cagaataagc
     8461 cacaaaaaac ctagaaacaa ctgtttgctc gggtccacag gaactcttat ttacagaaaa
     8521 cccatcttcg ctcaaaaaaa aaccattttt agtgaaaaat ggttttgtga ttctctaaaa
     8581 taaaagatag acttttatgc tttccgcaaa aaacgcaggt tttggttgat ttatgatcta
     8641 ctacacaaca acaaaaacgg actgtcttct cagtatcatg caatgcgttt ccaatggaaa
     8701 agcaaagttc tggttttctg atgttgtgcc tttttcgaag tttcaaaacc tgattccgaa
     8761 actaatttta gagtatggtc tggatctcgg tgagcaagcg cgtaagaaaa aatcagacta
     8821 cggggagcca gtgtggagtc ttgtgatcaa ttatgatccg gcaaaaagtg aggtgttcca
     8881 gttctggtta tttaccactg gttatcgtga agcacgaaga agcaagctga cgctaaaaga
     8941 aatactcgct aagaattcga gcatggcgcg caagcaaaag ctgaatagta ttttgacgac
     9001 taaaaaagaa aaactgctgc gttttggtcc ctatgtatta ggtcaatata ttgaattttc
     9061 ggatctgaaa actgaatttg cgaagatata ttatttccct gagaattatg gggtcgtttt
     9121 taatacgcat acaaaacgtg tgaaggcgat tgatagcaac aaaaagttta tctatcgaat
     9181 ctttaagccc tttgatgcct cagaagaacg acgatttaag agtttatatc gaaagtttgc
     9241 ttttgttttt ttagacaatg agcacaatcg ttggaaccaa gaatctgtga ctcagtttct
     9301 attggggttt gggattaaat ttgaaaatga tgagagttac aacgataggc taaaagagct
     9361 tacccgggtc ttacgtttgg ttcgaaaaaa gcatttagaa ttctttcagc gttattccac
     9421 taaaagagtc cggtttactt ggtatttatc ccaagacttc ttagatggat ctaaacgcga
     9481 gtttgaaaag agattaaaca agatccaaac tacgccagaa agtatagaga aatttgcaga
     9541 agccagcgca cgattgatgg cacatgggaa ttttcatggg actcgatttc aaattggttc
     9601 aatacaggca agggttcgaa aattattgaa tcagatacag cctgatcata agaagctgaa
     9661 taagaagtat ttcacgaacc atctgcacta tgtccgattt accaagaaaa aggcaatgag
     9721 cattaaagag tttgaaatgg cttgtcgtaa tgcagatacg atgtatttga acaaacagaa
     9781 taagcaaaaa cttaaagatt aaagattaaa ggtgaactat gctgaaatat cctgatgatt
     9841 atgatctcag ccgccaagaa attgacgatt tgagagctga gaccaaagat gcagcaaaat
     9901 atatgaaggc tttgttcaat tttaagattg aagcacgcta ttcatatagt gtgacgtggt
     9961 ctgcccaaga cggagaattt gttggccttt gtgccgaatt tccttcgctg tcctggctag
    10021 atccggacag cgataaagca cgctcaggca ttgaaagact tgtctttgac acattacaag
    10081 atatgaactc tactggagaa atcatacctt aatacttttg gaaaaatgcc tgattccgct
    10141 ggatgtattc attcagattg tttgaatcta agccatatat ttgcagtttt ttatcgatat
    10201 atttaaaaaa gaaagaataa acaatatgca taatcccaag cgcaaaagcc tctattttga
    10261 tcgccggtaa tagggcattt gggtctgtaa agagagtttc taacgtgaaa tgattaaaaa
    10321 aatagatgaa aaaagtcacc agactcaaca gagtcaaaag tgcataagct ttagcacaag
    10381 tcttgcagtg cccattcatt ttttctaatt tctgaaaaat tgtatgattc atttggctct
    10441 caaggcatct aattttttga atgatggact ctctacaaat tttaaatatt caggattaaa
    10501 aagggatagg aattttgtgt cgaatgatac tgtgcttcgt gaaaatttat tgaaattata
    10561 aagagttgag ttttaagtaa gatactgaaa ttaaataaca tcattaagat gatcacacat
    10621 aatacctacc tagtgaagca aatccaggta ggtattatgt gtgatgctaa actttaaatt
    10681 tttcacacaa tataagtatt tgattttaaa tgttattgta tttgtgtggg aagaataaaa
    10741 ctcaaacgtt aattttttaa gttattgaaa ttaaataaaa aatcaagatc acacataata
    10801 cctaccttga agtagaaatg aagttattat gtgtgttgca taacgcctca catgcagaaa
    10861 tgtctttagt tttatctcct gtctacccga cttagaatat gatttaggta ggctttactc
    10921 aggattacgt caatgtcagc agtaaatcta attaatgaaa atgacgatga gagagaaatt
    10981 gcttctcagg cagcttgtac acttcgagaa agctttgtta ccgcagctca aagtggacca
    11041 gttatgtatg ttgaaaacga tactgtactg cttaaggaac taaatggtcc cccaattgtg
    11101 attaaacaac tatctgggcg taatcctgaa cttgctcaac gagtagcgag tcgtggaact
    11161 tttaaaatta aaaaacgtaa agtaagccag gattgaagct cacggggact tacaattcaa
    11221 tcaaatttcg tataagagat tttatgttaa atgagagcta aaaaaatgcc taaattatag
    11281 gcatttttta tagtttatat attttaagtt gcattatttc tttgaataat ttcatgaaca
    11341 gtttttagaa tttgtaatga gattcctatt gatgaacctg atgggacttt taatatagag
    11401 tcctctgatt gatgtgctgg cgttattgca cgttgtgtta atactcctaa agcaacccct
    11461 tcttcattga caataggccc tccacttgca ccactatgca tcccattatc tatgtaaaaa
    11521 acgtcagttt ctattcttat attttgggag ttatcatcag caaaaatacg acgaatattt
    11581 ccaatataac catgtttaat tattggacct gtcatatctg ctaagtatcc ttcctctcta
    11641 attgctttaa taaacttatc tgcaccaaat gattctggat caataatttt ctcaatcgaa
    11701 aatgggatat taagttcatc tgagtagcct gcaacaaata ctctttttcc tagatttact
    11761 gaattaatac ttgcaggtag aaaggggaat ttgaaaggtt gctcttcttt tggtcgtaat
    11821 acagctatat ctagctgtat tatttcttta aaagatggga cgttaatttc aaatgagcaa
    11881 aaagatactg catattcaac taaaggtaac tcgggaaact ttatcaaaat tctttgctgt
    11941 ggatgagtgt aatcttcttt ttttataggc atcctaccag tcactacatg tgcagcagta
    12001 ataacttcac ctgtatccgt aaagctaaaa caagtacctt cacttataac ttcatcacct
    12061 tcaaaaactg tcacatatcc acaacagcgt tggatttttt catacaagtt tttaaacata
    12121 aaatttctca gtttttaata tcatttgaat ttttttatta tattttcgat aatcgatata
    12181 gcttgctcat aagcgataat attacctttt aggcaaaaat tacttacata gctgtgataa
    12241 ctgtccttcc aaagagtaga atcttttaga gtagctatag ttcttaaacc gtattcttgc
    12301 gcagatatcg ttatttcagt accacttcta tccctagtca tatcattaga tattttgctt
    12361 tcaattagag taattaattc tggtgagtca attgatagag cctctgccag tttgcatata
    12421 tcatataaat gcctaatgat ggtgttatct agtttttgac tatctgagtc ggcaatagtt
    12481 cgattacagt cagggattct ccaaattaat gcactgattt tatcggcaaa tatctgtcgt
    12541 gggtcagtac atgtaattct tttaatttct aattctctct gagaatagat gtgtaataga
    12601 gatccagcat ctctgtaaac aggttcaaaa tcttgcgatt ctttaaaaca aattt
//
