LOCUS       NZ_CP033125            12698 bp    DNA     circular CON 05-JAN-2024
DEFINITION  Acinetobacter wuhouensis strain WCHAW010062 plasmid p5_010062,
            complete sequence.
ACCESSION   NZ_CP033125
VERSION     NZ_CP033125.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN07138954
            Assembly: GCF_002165345.2
KEYWORDS    RefSeq.
SOURCE      Acinetobacter wuhouensis
  ORGANISM  Acinetobacter wuhouensis
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter.
REFERENCE   1  (bases 1 to 12698)
  AUTHORS   Hu,Y., Long,H., Feng,Y. and Zong,Z.
  TITLE     The complete genome of Acinetobacter wuhouensis strain WCHAW010062
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12698)
  AUTHORS   Hu,Y., Long,H., Feng,Y. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-OCT-2018) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
REFERENCE   3  (bases 1 to 12698)
  AUTHORS   Hu,Y., Feng,Y. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2017) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/04/2024 23:19:32
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,443
            CDSs (total)                      :: 4,336
            Genes (coding)                    :: 4,234
            CDSs (with protein)               :: 4,234
            Genes (RNA)                       :: 107
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 81
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 27 of 102
            Pseudo Genes (incomplete)         :: 73 of 102
            Pseudo Genes (internal stop)      :: 16 of 102
            Pseudo Genes (multiple problems)  :: 14 of 102
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP033125.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..12698
                     /organism="Acinetobacter wuhouensis"
                     /mol_type="genomic DNA"
                     /strain="WCHAW010062"
                     /isolation_source="Sewage"
                     /db_xref="taxon:1879050"
                     /plasmid="p5_010062"
                     /geo_loc_name="China: Sichuan, Chengdu"
                     /collection_date="2015-10-31"
     gene            join(11641..12698,1..418)
                     /gene="msr(E)"
                     /locus_tag="CDG68_RS00745"
                     /old_locus_tag="CDG68_00745"
     CDS             join(11641..12698,1..418)
                     /gene="msr(E)"
                     /locus_tag="CDG68_RS00745"
                     /old_locus_tag="CDG68_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000052512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(E)"
                     /protein_id="WP_000052512.1"
                     /translation="MSLIIKARNIRLDYAGRDVLDIDELEIHSYDRIGLVGDNGAGKSS
                     LLKVLNGEIVLAEATLQRFGDFAHISQLGGIEIETVEDRAMLSRLGVSNVQNDTMSGGE
                     ETRAKIAAAFSQQVHGILADEPTSHLDLNGIDLLIGQLKAFDGALLVISHDRYFLDMVV
                     DKIWELKDGKITEYWGGYSDYLRQKEEERQHQAVEYELMMKERERLESAVQEKRQQANR
                     LDNKKKGEKSKNSTESAGRLGHAKMTGTKQRKLYQAAKSMEKRLAALEDIQAPEHLRSI
                     RFRQSSALELHNKFPITADGLSLKFGSRTIFDDANFIIPLGAKVAITGSNGTGKTSLLK
                     MISERADGLTISPKAEIGYFTQTGYKFNTHKSVLSFMQEECEYTVAEIRAVLASMGIGA
                     NDIQKNLSDLSGGEIIKLLLSKMLLGKYNILLMDEPGNYLDLKSIAALETMMKSYAGTI
                     IFVSHDKQLVDNIADIIYEIKDHKIIKTFERDC"
                     /name="msr(E)"
     gene            474..1358
                     /locus_tag="CDG68_RS00750"
                     /old_locus_tag="CDG68_00750"
     CDS             474..1358
                     /locus_tag="CDG68_RS00750"
                     /old_locus_tag="CDG68_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mph(E) family macrolide 2'-phosphotransferase"
                     /protein_id="WP_000155092.1"
                     /translation="MTIQDIQSLAEAHGLLLTDKMNFNEMGIDFKVVFALDTKGQQWLL
                     RIPRRDGMREQIKKEKRILELVKKHLSVEVPDWRISSTELVAYPILKDNPVLNLDAETY
                     EIIWNMDKDSPKYITSLAKTLFEIHSIPEKEVRENDLKIMKPSDLRPEIANNLQLVKSE
                     IGISEQLETRYRKWLDNDVLWADFTQFIHGDLYAGHVLASKDGAVSGVIDWSTAHIDDP
                     AIDFAGHVTLFGEESLKTLIIEYEKLGGKVWNKLYEQTLERAAASPLMYGLFALETQNE
                     SLIVGAKAQLGVI"
                     /name="Mph(E) family macrolide 2'-phosphotransferase"
     gene            complement(1548..2159)
                     /locus_tag="CDG68_RS00755"
                     /old_locus_tag="CDG68_00755"
     CDS             complement(1548..2159)
                     /locus_tag="CDG68_RS00755"
                     /old_locus_tag="CDG68_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004889724.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_015060246.1"
                     /translation="MIEMNTRIYLRASTKDQDAERALQILQDLNQNLNLGETIVYVENY
                     SGTKLDRPELNKLLSEANQGDTLLVESIDRLSRLTQRDFQELKRRIQEKGLRLVVADLP
                     TTYQMIQTSDSITHSILELINNMLIDLLATMARLDNEKRIERIKQGLARSGYKPTGKKA
                     NEAKHKRIKELLVVGNMTKEEIAKAVNCGVATVYRVAKVI"
                     /name="recombinase family protein"
     ARG             complement(2386..3506)
                     /name="tet(39)"
                     /gene="tet(39)"
                     /gene_name="major facilitator superfamily (MFS) antibiotic
                     efflux pump"
                     /tool="rgi"
                     /drug_class="tetracycline antibiotic"
                     /identity="100.0"
                     /coverage="94.43"
     ARG             complement(2389..3572)
                     /name="tet(39)"
                     /gene="tet(39)"
                     /gene_name="tetracycline efflux MFS transporter Tet(39)"
                     /tool="amrfinderplus"
                     /drug_class="TETRACYCLINE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            3638..4279
                     /locus_tag="CDG68_RS00765"
                     /old_locus_tag="CDG68_00765"
     CDS             3638..4279
                     /locus_tag="CDG68_RS00765"
                     /old_locus_tag="CDG68_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016927662.1"
                     /GO_process="GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="WP_004787586.1"
                     /translation="MSKKDIAPQRLTREIVLRTALDMLNEEGIDSITTRKLAQRLGIKS
                     PTLYWHFKNKSLLMEAMAETIINEHHLVSLPIDGMTWQDWLLANSVSFRRALLAYRDGA
                     RLHARTSPSQGHFNTIEAQVALLSHAGFSPVEAVALLMTLGRFIVGWVLEEQQEEIRSD
                     PPFEADPTIYPLMLQGVNTLQNMNADDIFENGIRMVIIGAERQLDIKMQT"
                     /name="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
     gene            complement(4816..5571)
                     /locus_tag="CDG68_RS00775"
                     /old_locus_tag="CDG68_00775"
     CDS             complement(4816..5571)
                     /locus_tag="CDG68_RS00775"
                     /old_locus_tag="CDG68_00775"
                     /inference="COORDINATES: protein motif:HMM:NF016362.4"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0004519 - endonuclease activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="WP_087554563.1"
                     /translation="MAIELITENEFSEYVIKTFTGITKEFAWYRSNTLDLVGVLVQDNI
                     DKDWGYVILAKEEDGNYRPQDVGASIDSKSDANNELYAKMLAIEVSGEIKDELYDDNSV
                     SLDLPKIEIMSIDDHIKAYFKKHPEDLYQLSPRKFEELVASIIKDMGFDVELTPATRDG
                     GRDIIAYIRTGISSYLTHIECKRYAPDNKVSVDIIRSVVGVHHMRNANKSVIVTTGFFT
                     KDARDEAKLVENSLDLKDYNDLKDMLSKY"
                     /name="restriction endonuclease"
     gene            complement(5671..5997)
                     /locus_tag="CDG68_RS00780"
                     /old_locus_tag="CDG68_00780"
     CDS             complement(5671..5997)
                     /locus_tag="CDG68_RS00780"
                     /old_locus_tag="CDG68_00780"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_087554564.1"
                     /translation="MAAVSLSGTAIFEVEIENEEGDIEYEEIMISPDDVEWDTEVHDPD
                     RQMGTEYVHIGTAYVNGEEVQWLVYEYPEGILNYIDRQTNGLNLSKDFTIGLEFEAEQD
                     FEDF"
                     /name="hypothetical protein"
     gene            complement(6123..7019)
                     /locus_tag="CDG68_RS00785"
                     /old_locus_tag="CDG68_00785"
     CDS             complement(6123..7019)
                     /locus_tag="CDG68_RS00785"
                     /old_locus_tag="CDG68_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004931611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="WP_087554565.1"
                     /translation="MSLLIPIPRKQTYQTFFNVNSKSETQGVYQWHQDLAAEAFLILCD
                     FEIIFRSKIHKAMASLNCYPNQDDWIVGSHQQQNLVNKLQLDMLSAQQAGKDFYPDANN
                     YNLHSSFQLSKKDRQNFINLICEYIEKGKTSFTHDDLVASVSFGFWVSLLKRLEWLKKG
                     RLITPYLKEIFPHSMKGFDLNHLKSILDTLNRIKSFRNRIGHHDSIMRIPEPVVGHADF
                     YPRTVNQMINSLKILLLSLLDIVRDIDPQCSLAIEQSKNWKSFFLLLKIESFHVYRGNN
                     GNLESYVICYLNDCYST"
                     /name="Abi family protein"
     gene            7272..7754
                     /locus_tag="CDG68_RS00790"
                     /old_locus_tag="CDG68_00790"
     CDS             7272..7754
                     /locus_tag="CDG68_RS00790"
                     /old_locus_tag="CDG68_00790"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_087554566.1"
                     /translation="MNKTNISLIFSFLAIIIATWSAYTQYLQRQDAIEERIKVELKMTF
                     DKGLLSPTDLRILSGVDERKELKTAMLITNLGNAPVKIIEAGYQDLDMPEYASLPDPEK
                     VLSPGERVLLPMPNLITINHQLTDNIKMGVDKNAKIFAVTTKGKRFEVPAIIEVAK"
                     /name="hypothetical protein"
     gene            complement(7794..7964)
                     /locus_tag="CDG68_RS22405"
     CDS             complement(7794..7964)
                     /locus_tag="CDG68_RS22405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005200045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_162994885.1"
                     /translation="MSNIIQLSKPCAFCDSRESVQLFAGLMLCGNCQENIRITNPGMFE
                     AKDQIEQKAQD"
                     /name="hypothetical protein"
     gene            complement(7961..8347)
                     /locus_tag="CDG68_RS00795"
                     /old_locus_tag="CDG68_00795"
     CDS             complement(7961..8347)
                     /locus_tag="CDG68_RS00795"
                     /old_locus_tag="CDG68_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005200047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_087554567.1"
                     /translation="MNSTRTVAVRLDNQVAQEYERLAQLKGDKLGTYLRDLLTSNLQMA
                     SIERQASRIETVVNDFEKQINYSLDKFTEANYLNKKFFEDFGGVYMMLLGTLMHLKVER
                     DDIRGMQRQGNAYAQKNYFEGKKP"
                     /name="hypothetical protein"
     gene            9512..10441
                     /gene="repM"
                     /locus_tag="CDG68_RS00800"
                     /old_locus_tag="CDG68_00800"
     CDS             9512..10441
                     /gene="repM"
                     /locus_tag="CDG68_RS00800"
                     /old_locus_tag="CDG68_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001292331.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepM"
                     /protein_id="WP_087554568.1"
                     /translation="MTDLVVKSNKLVQALQTLTLSETRLLQLAIVDARETGQGLSTDEP
                     LELSAIRYAKAFNVSPDAAYLALIEAEDSLFKRQFTITNEDGSLTKSRWIQDANYRKGE
                     GRILVTLTRVVIEHVTKIDGFEQYFTSYHLRKTSEFKSVYAVRLYELLMQWKSIGKTPL
                     FELNKFRSQLGIGVNEYTRMEAFKRRVLDIAIDQINEFSDINLKYEQHKKGRSISGFSF
                     SFKQKKSTHEHIESKRDPNTLDAFSKMTDAQRHLFSNKLSELPEMSKYSQGTESYQQFA
                     IRIAEMLQDPTRFEELHPYLQKVGFKAA"
                     /name="repM"
     gene            10520..11038
                     /locus_tag="CDG68_RS00805"
                     /old_locus_tag="CDG68_00805"
     CDS             10520..11038
                     /locus_tag="CDG68_RS00805"
                     /old_locus_tag="CDG68_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017397202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication DNA-binding protein"
                     /protein_id="WP_087554569.1"
                     /translation="MKSLSVLELSKLYSINRQTIYNQINKGILSKNSDNKIDLAEAIRV
                     FGEPIRKQDVKEPVKVDSPNSAEVLLLRQQIDILKNQLDDAKDRESFYQNQIETMQRLL
                     EAPKPHIPTSIDPEPTQAEPMTEEKEEVIAMPQKELEATENKRIPVPEHVEQEPEKRGF
                     WSRFFRPYD"
                     /name="plasmid replication DNA-binding protein"
     ARG             475..1355
                     /name="mph(E)"
                     /gene="mph(E)"
                     /gene_name="Mph(E) family macrolide 2'-phosphotransferase"
                     /tool="amrfinderplus"
                     /drug_class="MACROLIDE"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             11642..12696
                     /name="msrE"
                     /gene="msrE"
                     /gene_name="msr-type ABC-F protein"
                     /tool="rgi"
                     /drug_class="macrolide antibiotic; streptogramin
                     antibiotic"
                     /identity="100.0"
                     /coverage="71.69"
     ARG             3..418
                     /name="msrE"
                     /gene="msrE"
                     /gene_name="msr-type ABC-F protein"
                     /tool="rgi"
                     /drug_class="macrolide antibiotic; streptogramin
                     antibiotic"
                     /identity="100.0"
                     /coverage="28.11"
     ARG             475..1358
                     /name="mphE"
                     /gene="mphE"
                     /gene_name="macrolide phosphotransferase (MPH)"
                     /tool="rgi"
                     /drug_class="macrolide antibiotic"
                     /identity="100.0"
                     /coverage="100.0"
ORIGIN
        1 aaaagctgaa attggctact ttacacaaac aggatataaa tttaacacgc ataaatctgt
       61 gctctccttt atgcaggaag agtgcgagta cacagttgcg gaaattcgtg cagtattggc
      121 ttcaatgggg atcggagcga atgatattca aaaaaactta tccgacttat cgggaggtga
      181 aatcatcaaa ctgcttttat ccaaaatgct tttaggaaaa tataatattt tgcttatgga
      241 tgaaccagga aactatcttg acctaaaaag tattgccgca ttagaaacaa tgatgaagtc
      301 ctatgcagga actattatct tcgtatctca tgacaagcaa ttggtcgata atattgctga
      361 cattatctac gagatcaaag accacaaaat catcaagact tttgagagag attgttaatg
      421 atagccaatc taatccgaac attaattatt gaactcttta aaggaaatta aaaatgacaa
      481 ttcaagatat tcaatcactt gctgaagcac acggcttgtt gcttacggac aaaatgaatt
      541 tcaatgaaat gggcattgat tttaaggtcg tttttgctct tgatacaaag gggcaacaat
      601 ggttgctgcg tattcctcgt cgtgatggca tgagggaaca aatcaagaaa gaaaaacgca
      661 ttttagaatt ggtaaaaaaa catctttctg tagaggttcc tgattggaga atttcatcta
      721 cagaattagt ggcttatccc atacttaaag ataatcctgt tttaaatttg gatgctgaaa
      781 cctatgaaat aatttggaat atggacaaag atagcccgaa atacataaca tctttggcaa
      841 aaaccttatt tgaaatccat agtattcctg aaaaagaagt tcgggaaaat gatttgaaaa
      901 ttatgaaacc ttcagattta agacctgaaa tagcaaacaa tttgcagtta gtaaaatctg
      961 aaattggtat aagtgagcaa ttggaaaccc gctacagaaa atggttggat aatgatgttc
     1021 tatgggcaga tttcacccaa tttatacatg gcgatttata tgctgggcat gtactagctt
     1081 caaaggatgg agctgtttca ggcgttattg attggtcaac agcccatata gatgacccag
     1141 cgattgattt tgctgggcat gtaactttgt ttggagaaga aagcctcaaa actctaatca
     1201 tcgagtatga aaaactaggg ggtaaagttt ggaataaact atatgaacag actttagaaa
     1261 gagcagcggc ctctcctttg atgtatggtt tatttgcctt agaaactcaa aatgaaagcc
     1321 ttatcgttgg agcaaaagct cagttgggag ttatataatt taaaaatatg attgctgaga
     1381 actgccttgt tttgaaactt ggttggcttt aattagtttt tagtattctt tatagaaaat
     1441 gcctcgatca aggggcattt ctaacaatca tttaacataa aatttcttat gtgaagtaac
     1501 ctgaatgcag cggtgcccca ttgggggcgc gttgagataa gcctctttta gataacttta
     1561 gcaactcgat agacagttgc aactccacaa ttcactgctt tggcaatttc ttccttagtc
     1621 atattgccaa ctactagcaa ttcttttatt cgtttatgtt tagcctcatt tgccttcttg
     1681 cctgttggtt tgtaacccga acgtgccagg ccctgcttaa tacgttctat acgtttctca
     1741 ttgtctaggc gggccattgt tgccagaaga tcaatcaaca tattgttgat gagttccaag
     1801 attgaatgag taatgctgtc actggtttga atcatttggt aggtagtagg aagatctgct
     1861 acgactaaac gaagtccctt ctcctggatc ctgcgcttca gctcttgaaa atctcgttgg
     1921 gttaggcgtg ataagcggtc aatactttca actaacaatg tgtcaccttg atttgcttct
     1981 gacagtagct tattcaattc aggtcggtct aacttcgtac cactatagtt ttccacgtac
     2041 acaatggttt cacccaaatt caagttttgg tttagatcct gaagaatctg caaagccctt
     2101 tctgcatctt gatctttagt tgaagctcgt agataaatac gtgtattcat ttctatcatt
     2161 taatcttaat tctattagat taatgataat actatcattt aattattgca ttaagtctaa
     2221 tgatagatat tgtatgcgat ttggctcgct atcattcagg tatagtcttt tgataaaaaa
     2281 agcatgacta aaagccatgc cttgggaatt cgtataacgt gtattatgtt aattttagat
     2341 aaactataga atccattagt ttttaaacca ataaattaaa tattttatgg aactccagaa
     2401 tatttattca ggcgatactt aagcaataag ggtagggtga ttacataaag tattgcagct
     2461 aatgcccata ccattccagg agctgatgcc tgagtcataa aatagaggct agtaactaat
     2521 acaggaccaa ttatagcccc cagactatta aaactgctta atagcccttg taattcacct
     2581 tgtctttcat cactaatacc tctggacatc attgcttgca aagcaggaac ggctactcca
     2641 ccaagggcaa acaatggtaa caacgcaaaa ggcacccatt cttcaacagc aatagataaa
     2701 agaagtaaac caattgcatc gcaagctatt ccacataaca acgtattttt ctctccaaac
     2761 ctctttacca taggggcgat agcaaaagcc tgtgcaatag aatggcatat tccataacta
     2821 gcaagggaaa cacctgcaat aaatatactc cagccataac gatcctgccc atagataacc
     2881 cataaagttg cggggacttg tgaaaccaat gtgataatta aaaagatacc aagcaatgga
     2941 gccatattag gttgagtgat caaataggat aatttcttta atattgattg ctcctgaacc
     3001 ttctcagtca ttttattgct gtgttttgat tcttttaata agactgctgt cattattaaa
     3061 ttaatcccat tcataaaagc agcagcaaag aacggtgcat gtaagttcca ttcacccaaa
     3121 actccaccta ttaccggacc cgctataaac ccaataccaa agacagctcc aaggagacca
     3181 aacctttttg cacgctcatg ggctggagta atatctgaaa cataagctgt tgcaacagcc
     3241 atgttggcac ctgtaattcc cgcaaaaata cgaccaatat atagccacaa tagagaagga
     3301 gcagcagcca ttaggagata atcagccgtt gcaccagcaa ttgaaataat taatacaggt
     3361 cgacgtccaa atcggtcact caacgctcca aggataggtg caaaaatgaa ctgcatcaga
     3421 gcatacacag ctaataaagc accatagtga acaccgcctg cttcagctcc agccaatgac
     3481 cgtaataatt caggcaagat cggcataatt aaaccaatcc caacagcatc cagaaatata
     3541 gtgattaaaa tcacgctcaa tgatttcttc acaaatgcct tctctagcaa tgatagaatt
     3601 aatattctct cacacactcc ctatcagtga tagatttatg agtaaaaaag atattgcacc
     3661 tcaacgtctg acacgtgaaa tagtcctgcg taccgcattg gatatgttga atgaagaagg
     3721 catagattca ataaccacac gcaagctagc tcaacgattg ggtattaaat ctccaactct
     3781 atattggcat ttcaagaata aatccttgtt aatggaagca atggcggaaa ccattataaa
     3841 tgaacaccat ttagtttctt tacccatcga tgggatgaca tggcaagatt ggttgctcgc
     3901 taattctgta agctttcgtc gagcactact agcttaccgt gatggtgctc gtctacatgc
     3961 aagaactagc cccagtcaag gacatttcaa cacgattgag gcccaagttg cgctgttatc
     4021 tcatgctggg ttttctcctg ttgaagcagt tgcattactt atgacattag gaaggtttat
     4081 tgttgggtgg gtccttgagg agcagcaaga agaaataaga tcagacccac cattcgaagc
     4141 cgatccaact atatatccac tcatgctcca aggagtgaat accttacaaa atatgaatgc
     4201 ggatgacatt tttgaaaatg ggattcgtat ggtgattatc ggtgctgaaa gacaacttga
     4261 tattaaaatg caaacttaac tctatactgc tttaagcaac tgttctattt agtaataaag
     4321 cttttatcct taattattta acataataga gcttatacga aacgtgtttt taatattact
     4381 ttaagatcag tatcttatcg ttattcatac cctttggaac gttctcgttc aaccgacttg
     4441 gaaacaagtc ggtttctttt taagcaaaac tgtaactttt agccaataaa gttgatcaaa
     4501 aaacattcgt ttttgcattt tttcctgatt tctcaatggt ccagattcag aaataatccc
     4561 caatttctgc ggacaactct tgggataaaa taggtcaact ttgtaagcca aagcgtacat
     4621 agatttagat aatttgcgga ttggataact aaaatcttag tcctatgatc agacataagg
     4681 gaactggatg tttcccaaaa aataagaata agaaaattgt tcaaggaatg ggcctagctc
     4741 taacggtata acgcacaacc caatgtaaaa aggcccgaca ggatgtcggg cttttttatt
     4801 tgtattatca atttattagt acttacttag catgtctttt aaatcattat aatcttttag
     4861 atcaagacta ttttcaacta acttggcttc atcacgagca tcttttgtaa aaaatcctgt
     4921 tgtaacgatg acgctcttat ttgcatttcg catgtgatgg acaccaacaa cactacgaat
     4981 aatatctaca ctaactttat tgtcaggcgc gtaccgttta cactctatat gcgttaaata
     5041 actagagata cctgttctaa tatacgcgat aatatctcta ccaccatcac gagttgctgg
     5101 tgtcaattca acgtcaaatc ccatgtcttt aataatacta gcaactaatt cctcgaattt
     5161 tcgaggtgaa agctgataaa ggtcttcagg gtgttttttg aaatatgcct taatatgatc
     5221 atcaattgac ataatttcta ttttaggtag atctagagag acactattat cgtcatatag
     5281 ttcatcttta atctctccag atacttcaat agctagcatt tttgcatata actcgttatt
     5341 tgcatctgac ttgctatcta tacttgcacc aacatcttga ggtctatagt taccatcttc
     5401 ttcttttgcg agaattacat atccccaatc tttatcgata ttgtcctgaa caagaacacc
     5461 aacaagatct aaggtattac ttctatacca tgcaaattct ttcgttatac cagtaaatgt
     5521 tttaattaca tattcagaaa attcattttc tgttatcaat tcaatagcca atttatcacc
     5581 taactaacta gtttttcata aaataaggat cacttttatc taaaaaaaaa gaagcagctt
     5641 aataggctgc ttccttaaat cagatgaaaa ttaaaaatct tcaaaatcct gctctgcttc
     5701 aaactcaaga cctattgtga aatcttttga taaattaagt ccattggttt gacgatctat
     5761 ataattcaat atgccttctg gatactcata tactagccac tgaacctctt caccatttac
     5821 atacgctgtt ccaatatgta catattcagt ccccatttgt ctatctgggt catgtacttc
     5881 tgtatcccat tctacgtcat caggggatat cataatttct tcatattcaa tatcaccctc
     5941 ctcattttct atttctactt caaagatagc tgttccactt aaacttaccg cggccatatt
     6001 tattcctttt aactgttgtt cttacaaaac ctatataata ctaaatattt aaaattcaat
     6061 aggaaatcta taatcctttg gcttcaatag caaagctaaa aaaaacactc tcaattactt
     6121 tatcatgtac tgtaacaatc atttaaataa caaatgacat agctttccaa attcccatta
     6181 ttaccccgat atacatggaa actttcaatt ttcaacaata gaaaaaagct cttccaattt
     6241 ttagattgtt caatagctag gctacactga ggatcaatat cacgaacaat atctaataaa
     6301 cttaacaata atattttcaa gctattgatc atctgattta cagttcgggg ataaaaatca
     6361 gcatgcccca ctactggctc tggtattcgc ataatactat cgtgatgacc aatacgattt
     6421 ctaaatgact taattctatt taatgtatct aagatacttt ttaaatgatt taagtcaaaa
     6481 cctttcatgg aatggggaaa tatttctttt agatatggag ttattaatct tccttttttc
     6541 aaccactcca aacgtttgag taaactcacc caaaagccaa aactaacaga tgcaaccaga
     6601 tcatcatgag tgaacgaggt ttttcctttc tcgatatatt cacaaattag gtttataaaa
     6661 ttttgtctat cttttttact taattgaaat gatgaatgta aattatagtt gttagcatca
     6721 ggataaaaat cctttcctgc ctgttgggca ctaagcatat ctagttgaag tttattaact
     6781 aaattttgtt gctgatgact acctacaatc caatcatcct gatttggata acaattcaaa
     6841 gaggccatag ctttatgaat tttactccga aatataattt caaaatcaca taagattaag
     6901 aatgcttcgg cagctagatc ttgatgccat tgatagaccc cttgtgtctc acttttagaa
     6961 ttaacattaa aaaaagtttg ataagtctgc tttcttggta ttggaataag tagtgacatt
     7021 gagctaaatg attgcaatca gttttaaatt gcgttaaatt gtacacgtta agtcttatta
     7081 attacggttg ataggacaaa aaaattgagt aaaaagcccg cttaatagtg ggctttttgc
     7141 tgtctatggt ttattcactt aacataaaat atctttttca caagataaac aagaaaattc
     7201 tctacaagcc tgtgatgact atcgcacaac catatgtatt acataaatat ttttaaaatg
     7261 gttatacttc aatgaataaa actaatatct ctctaatctt ttcgttccta gctattatta
     7321 ttgctacttg gtctgcctat actcagtatt tacaaagaca agatgctatt gaagaaagaa
     7381 tcaaagtaga attaaaaatg accttcgata aaggcctttt aagcccaaca gatttaagaa
     7441 tattgagtgg ggtagatgaa agaaaagagc taaaaactgc tatgttaatt acaaatcttg
     7501 gaaatgcccc cgttaaaatt atagaagcag gctatcagga tctggatatg ccagaatatg
     7561 catctctacc agatccagaa aaagtcttat ctccaggtga gagagtctta ctccctatgc
     7621 caaacctcat taccatcaat catcagctca ctgacaatat aaaaatgggg gtggataaaa
     7681 atgctaaaat ttttgcagtg acaacaaaag ggaaacgctt tgaggtacct gcaataattg
     7741 aagttgcaaa gtagtttttt atttaacata aaatctctta tacgcagccc aggttaatcc
     7801 tgggcttttt gttcaatctg atccttggcc tcaaacatgc caggattagt gatccggatg
     7861 ttttcctgac aatttccgca aagcattagc cctgcaaaaa gctgtacgct ttcccttgaa
     7921 tcacagaaag cacatggttt agagagttga ataatattgc tcatggcttt ttcccttcaa
     7981 aatagttctt ttgggcatac gcatttcctt gtctctgcat accgcgaata tcatctcttt
     8041 ctacttttaa atgcataagc gtaccgagta gcatcatgta cactccacca aaatcttcaa
     8101 agaatttttt gttcagataa ttggcttcag taaacttatc gagggaatag ttaatctgct
     8161 tttcgaaatc attcactact gtttcaatcc tactggcctg ccgttcaata ctggccatct
     8221 gtaaattact ggtcagaaga tcccgcagat aagtccctaa tttatcgccc ttcaattgag
     8281 ccaaacgttc atattcttgt gcaacctgat tgtctaatct gacggcaacg gttcgagtac
     8341 tgttcatgaa aaactcctat catttgtttt caatctaagc acctaatttc taaaaagtaa
     8401 ctatcaaata taagtaaaca ctgtgttaag ttttaatgat tacaggttaa atatctaata
     8461 taaaaagaga aattaatata aaagcgtaaa caaagctgat tacaagctat cttattgttt
     8521 tgtaagtaaa tattgcactt tacctgcaac aaaaagtgtt tgcggctaag cctatgaaag
     8581 ataataaaaa tcatgataaa agcgtaaaca ctttccagac agatgaaaaa tcgctgtatc
     8641 ccttatagca taaggctttg gagcttttat ctttagatgc gtggtgtgtg aaccaaaaat
     8701 tgccggtatg taccggctcc caatccattt cattgactga actgacagta aagatagtgc
     8761 ttggccaaaa gagatcctgg ctattgtgtt tctaagagca aaatctataa gcgcaaactc
     8821 tagcctacag atcaaaaata aaacccaagt taaaacgctc ttacacgcga tttcagccac
     8881 atttttactt aagacataga atccactatt gaaatgagta atcaggtcaa aaaacgcaaa
     8941 tgagcgccaa atagaggcta tttttatcct aatcgggatg aatagagcga gtgtctacga
     9001 gcgaagcaac aaaattcgca cctcgactct ctgaaaaaca gctttttagg ctgtgaagcc
     9061 taaataagcg taaatcacat gcagaaaatt gagcctagat taagcgtagc gagtaaaaaa
     9121 gctttatgag cgaagcgaat tccgagttgc ttttgctctt gcctttgctt tttctcaaag
     9181 tcacaaccgg atgatctaaa aaattgcccc acgaatcgag cgaaagcgag attcaataga
     9241 gtttgagcgt agcgaaaacc aagggcaatt tttctttgcc agggctttta ataattttta
     9301 aaattttaaa tgcttttaga cctgctgtaa gcctttatac aaaagagttt cctcggttat
     9361 atagcaacgt ttcctcggtt atatagcaac gtttcctcgg ttatatagca acgtttcctc
     9421 ggttatatag caacgtttcc tcggtatttg acaacgcatt ttgattatgt tgttatagca
     9481 acacaataaa accgttgcta aataaaacaa catgacagac ttagtcgtaa aatcaaataa
     9541 acttgttcag gccttacaga ctctaacctt aagcgaaact cgtcttttac agctcgctat
     9601 tgtagatgct cgggaaacag gacaagggtt atccactgat gaaccattag aactaagtgc
     9661 cattagatat gcgaaagcct ttaatgtttc gccagatgcc gcttatttag ctttgattga
     9721 agcagaagat tcgctattta agcgacaatt taccattaca aatgaagatg gaagtctaac
     9781 gaaaagtcgt tggattcaag atgcaaacta tcgaaaaggt gaaggtcgaa ttctcgttac
     9841 attaactcgt gtagttatcg aacatgtaac taaaatagat gggtttgaac aatatttcac
     9901 aagctatcat ctacgaaaaa catctgaatt taaaagcgtc tatgcagttc gtctgtacga
     9961 attattaatg cagtggaaat ctatagggaa gacccctctt tttgaattaa ataaattccg
    10021 tagccaattg ggtattggtg taaacgaata caccagaatg gaagcattta agagacgtgt
    10081 acttgatatt gctatagacc aaattaacga gttttcagat atcaatctga agtatgaaca
    10141 gcataaaaaa ggtcgctcaa tttcaggttt ttcattcagt tttaaacaaa agaaatcaac
    10201 tcatgagcat atcgaaagta agagagatcc taatacctta gatgctttct caaaaatgac
    10261 agatgctcaa cgtcacttat tttctaataa actttcggaa ttacctgaaa tgagtaaata
    10321 ctctcaggga accgaaagct atcagcagtt tgccatacgc attgctgaaa tgcttcaaga
    10381 ccctactaga tttgaagaat tacatcctta tcttcaaaaa gttgggttta aagctgccta
    10441 aattatagac ttgatcattt gtctattaga cagtctaata gacatcaact tttgacaaga
    10501 ttaggcatag ttttacacca tgaaaagtct ttcagttctt gaattatcta agctttacag
    10561 cataaataga caaacaattt acaaccaaat aaataaagga attttaagta aaaattctga
    10621 taataagatt gatttggctg aagctattcg agtatttggt gaacctatta gaaaacagga
    10681 tgtaaaagaa cctgtaaaag tggacagtcc aaattcggca gaagttttac tgcttagaca
    10741 gcagatagac atactaaaaa atcagctaga tgatgcaaaa gatagagaat cgttctatca
    10801 gaatcagatt gaaacaatgc aacgtctttt agaggcccca aaacctcata tccctacgtc
    10861 tatcgatcca gagcctactc aggctgagcc gatgacagag gaaaaagaag aagttattgc
    10921 catgccgcag aaagaactgg aggcgactga aaataagcgg attccagtgc cggagcacgt
    10981 tgaacaggaa ccggaaaaaa ggggcttttg gagccgtttt tttagaccct atgattaacc
    11041 acgattgttt aactaatatg aacaaagtcg tgaaacattg cttaaataat aggcgtggaa
    11101 cgtgaaaaat cataaaaaaa gcccaccaga gggtatgtgg gctaaaatca tgtaactaca
    11161 gcattgattt ctaaagataa ttataacgca tttcgtataa ggtgtattat gttaatttta
    11221 gaaaatcttc actactatta ttttaaaata agccttatat ccaagcataa aacaaggttg
    11281 tctagacttc ttttaacagt aaagttatca taaaactgaa ttttattttt taggtaagtt
    11341 tatgcattct atccgcattc gttaagacac aactatttgc atagtgacac tattttataa
    11401 tggtgggctt ttgttgtgtc tttaagaata tatgcggata tataaagtaa aagtatgctt
    11461 aatttataag tatgctttta gtgcatagtt tccagttata acttaattga ctagctattt
    11521 gtccaccctg tggatgaata gctttttttt tgggaggaca ctgtgatgct agcttttgtt
    11581 ttcacctaaa tcctgtttgc tgcataaaaa atttcaagag ctaaacagga gtaaataaaa
    11641 atgagtttaa ttattaaagc gagaaacata cgcttggatt atgctgggcg tgatgttttg
    11701 gatattgatg aattggaaat tcactcttat gaccgtattg gtcttgtggg tgataacgga
    11761 gcaggaaaga gtagtttact caaagtactt aatggcgaaa ttgttttagc cgaagcgaca
    11821 ttacagcgtt ttggtgattt tgcacatatc agccaactgg gcggaatcga aatagaaacg
    11881 gtcgaagacc gggcaatgtt atctcgcctt ggtgtttcca atgtacaaaa cgacacaatg
    11941 agtggcggag aggaaactcg tgcaaaaatt gctgccgcat tttcccaaca agtacatggc
    12001 attctagcgg atgaaccaac cagccacctt gatctcaatg gaatagatct acttattggt
    12061 caacttaaag catttgatgg agcattactt gttatcagtc atgaccgata ttttcttgat
    12121 atggttgtag acaagatatg ggagttaaaa gacggtaaaa ttacggaata ttggggtggt
    12181 tactcggatt acttgcgtca aaaagaagaa gagcgacaac accaagccgt agaatatgag
    12241 ctgatgatga aggaacggga gcgattagaa tctgctgtgc aagaaaaacg ccagcaagct
    12301 aatcgattag acaataagaa aaaaggagaa aaatccaaaa actctaccga aagtgctgga
    12361 cgacttgggc atgcaaaaat gactggcacc aagcaaagaa aactgtatca ggcagctaag
    12421 agtatggaaa agcgtttggc tgcattagaa gatattcaag caccagagca tttgcgttct
    12481 attcgttttc gtcaaagttc agccctagaa ctgcacaata agttcccgat tacggcagat
    12541 ggtctgagct taaaatttgg tagccgtact atctttgatg acgctaactt tataataccg
    12601 cttggcgcta aagtcgctat aactggatcg aatggaacag ggaaaacgtc cttgttaaaa
    12661 atgatatcag aacgtgctga tggattaacc atatctcc
//
