LOCUS       NZ_CP032896             9639 bp    DNA     circular CON 27-APR-2024
DEFINITION  Enterobacter kobei strain WCHEK045523 plasmid pQnrS2_045523,
            complete sequence.
ACCESSION   NZ_CP032896
VERSION     NZ_CP032896.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN08027255
            Assembly: GCF_003665375.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter kobei
  ORGANISM  Enterobacter kobei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 9639)
  AUTHORS   Wu,W., Long,H., Feng,Y. and Zong,Z.
  TITLE     The complete genome of Enterobacter kobei strain WCHEK045523
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9639)
  AUTHORS   Wu,W., Long,H., Feng,Y. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-OCT-2018) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_003665375.1-RS_2024_04_27
            Annotation Date                   :: 04/27/2024 03:02:54
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,870
            Genes (coding)                    :: 4,741
            CDSs (with protein)               :: 4,741
            Genes (RNA)                       :: 119
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 42 of 129
            Pseudo Genes (incomplete)         :: 82 of 129
            Pseudo Genes (internal stop)      :: 32 of 129
            Pseudo Genes (multiple problems)  :: 24 of 129
            Pseudo Genes (short protein)      :: 1 of 129
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP032896.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9639
                     /organism="Enterobacter kobei"
                     /mol_type="genomic DNA"
                     /strain="WCHEK045523"
                     /host="Homo sapiens"
                     /db_xref="taxon:208224"
                     /plasmid="pQnrS2_045523"
                     /geo_loc_name="China: Sichuan, Chengdu"
                     /collection_date="2017"
     gene            1..864
                     /locus_tag="D9T11_RS01175"
                     /old_locus_tag="D9T11_01175"
     CDS             1..864
                     /locus_tag="D9T11_RS01175"
                     /old_locus_tag="D9T11_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014003865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase RepA family protein"
                     /protein_id="WP_012537710.1"
                     /translation="MAINIMEAFQQEPPQLDFIWPGFLAGTVGALVAPGATGKSFWALE
                     AAMSIACSVAGGDVVNLKPQHTGRVVYLAGEDPEPALIRRVHAIGQHLNQSAREAIAEN
                     LTLEPIMGKRLNVMDEEHLRRVIEYSAGARLIVLDTLSRIHSLDENSNGDMARLVATLE
                     HVAASTGASVLYLHHVSKGSAREGQTDQQQAARGASALIDNARWCGFVAKMTEDEAKRL
                     SDRAYDRQPIGNDRRGFFVRFGVSKQNYDATPLDQWYERRDGGVLVPVELLEVPNGKKG
                     GSREQA"
                     /name="helicase RepA family protein"
     gene            851..1732
                     /gene="repC"
                     /locus_tag="D9T11_RS01180"
                     /old_locus_tag="D9T11_01180"
     CDS             851..1732
                     /gene="repC"
                     /locus_tag="D9T11_RS01180"
                     /old_locus_tag="D9T11_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014003864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein C, IncQ-type"
                     /protein_id="WP_012954629.1"
                     /translation="MSKHDLTHARHDPAHCLAPGLFRSLKRGERKRGKLDVVHDYGDGK
                     RIEFSGPEPLGADDLRILQGLVAMAGPGGLVLSPEPQTEAGQQLRLFLEPKWEAFQQDA
                     MVVKGSYRALAREIGYANPDDTKPVRECIERLWKVSIIAQNGRKRQGFRLLSEYASDEQ
                     DGKLYVALNPLIARAVMGGAQHVRIDMDEVRALQTDPARLMHQRLCGWIDPGKSGRVEL
                     DTLCGYVWPGQATNPNTLKTRRQAARRALPELAALGWAVSEYAKNKFEIGRPKATHLLG
                     PAPKTQEISHEK"
                     /name="repC"
     gene            1722..1973
                     /locus_tag="D9T11_RS01185"
                     /old_locus_tag="D9T11_01185"
     CDS             1722..1973
                     /locus_tag="D9T11_RS01185"
                     /old_locus_tag="D9T11_01185"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_064163397.1"
                     /translation="MRNDWETPPTVEVRIPVKLNSDSGICEHHFRKVCGQGFFEQKLGF
                     EGRHLFDSLNKPAAKRSAAVVDPRRWNRTVHLTSEYVL"
                     /name="hypothetical protein"
     gene            complement(2276..2932)
                     /locus_tag="D9T11_RS01190"
                     /old_locus_tag="D9T11_01190"
     CDS             complement(2276..2932)
                     /locus_tag="D9T11_RS01190"
                     /old_locus_tag="D9T11_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001516695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolone resistance pentapeptide repeat protein
                     QnrS2"
                     /protein_id="WP_012537714.1"
                     /translation="METYRHTYRHHSFSHQDLSDITFTACTFIRCDFRRANLRDATFIN
                     CKFIEQGDIEGCHFDVADLRDASFQQCQLAMANFSNANCYGIELRECDLKGANFSRANF
                     ANQVSNRMYFCSAFITGCNLSYANMERVCLEKCELFENRWIGTHLAGASLKESDLSRGV
                     FSEDVWGQFSLQGANLCHAELDGLDPRKVDTSGIKIASWQQEQLLEALGIVVFPD"
                     /name="quinolone resistance pentapeptide repeat protein
                     QnrS2"
     gene            complement(3659..5236)
                     /locus_tag="D9T11_RS01195"
                     /old_locus_tag="D9T11_01195"
     CDS             complement(3659..5236)
                     /locus_tag="D9T11_RS01195"
                     /old_locus_tag="D9T11_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing methyl-accepting chemotaxis
                     protein"
                     /protein_id="WP_101862731.1"
                     /translation="MRQNLPVTGRNLELPKDTNILSTTSPQSHITYVNPDFIKISGFTE
                     EELLGQPHNIVRHPDMPPAAFEHMWSTLKSGRSWMGLIKNRCKNGDHYWVSSYVTPIAK
                     NGSIVEYQSVRTKPEPEQVLAAEKLYAQLRSGKAARPKLAASFSVKILLLIWGSIISSA
                     MAAGMLTDTSISSLLLATLMSGSLSSVSVLAILSPLGRLVERARNISNNPLSQSIYTGR
                     TDEFGQIEFALRMMQAETGAIVGRIGDASNRLSEHTRGLLKDIESSNVLTVEQQAETDQ
                     IATAVNQMAASIQEVASNAQHAADAAGRADTETASGQRLVAHTSQSITALEGEIRQAAQ
                     VIHELEGQSNEISKVLDVIRGIAEQTNLLALNAAIEAARAGEQGRGFAVVADEVRSLAA
                     RTQQSTTDIQSMISALQERAQSAVTVMEQSSRQADTSVAHAEEAATALDGIGQRVNEIT
                     DMNAQIATAVEQQGAVSEDINRSIINIRDAADTNVQTGQNNLQSAKSVAQLTSALSELA
                     KQFWEKRG"
                     /name="PAS domain-containing methyl-accepting chemotaxis
                     protein"
     gene            complement(5788..6255)
                     /locus_tag="D9T11_RS01200"
                     /old_locus_tag="D9T11_01200"
     CDS             complement(5788..6255)
                     /locus_tag="D9T11_RS01200"
                     /old_locus_tag="D9T11_01200"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_012954630.1"
                     /translation="MQFKRQGRRVQVLAYRGYDKEKRRAIVKMMGSIDVYSYEPSGGLI
                     ENLTDEEKTELQSYIETERQAAEKRSRVYYAKSAASRIVEVADTIKAGDFEPSEAWAAD
                     TWAAIEALTKAMRKAGYPKLRKAPQKAADAPMPGQAGLPFGDAPETPESAS"
                     /name="hypothetical protein"
     gene            6713..7144
                     /locus_tag="D9T11_RS01205"
                     /old_locus_tag="D9T11_01205"
     CDS             6713..7144
                     /locus_tag="D9T11_RS01205"
                     /old_locus_tag="D9T11_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004171598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="WP_012537712.1"
                     /translation="MNTDESKRRRRGPAPLDATDKRGHTVSVRLNDAELARLDSQRDAV
                     QMQRGEYLRAAALHRLPPTIPAANREQWAELARTAANLNQIARHLNEGQRGDGERIGKA
                     LGLQLSAELANCLRLLVLVRGDLIGVKHDSEDVEDESEG"
                     /name="plasmid mobilization relaxosome protein MobC"
     gene            complement(7335..7568)
                     /locus_tag="D9T11_RS25760"
     CDS             complement(7335..7568)
                     /locus_tag="D9T11_RS25760"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_229025479.1"
                     /translation="MLKLLGRLDGAAGFPLAQPNFPVQPYPAGHDVDVILVRVLVAHCH
                     PRRIGLVKAHALHEVSRHGFPLLGAQALTGRQ"
                     /name="hypothetical protein"
     gene            7900..8583
                     /locus_tag="D9T11_RS25765"
     CDS             7900..8583
                     /locus_tag="D9T11_RS25765"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_043849885.1"
                     /translation="MTKLETVRALNDSGPQLQIVESVRTLQQSLSLMHEDLKRLPVAVS
                     TETAQTLEPLARLQQDVTQVLEAYDKVTAIQRKTLDELTQQMSASAAKAFEQKAGELDK
                     TISDLSNSLSGLETSISSMKQTAEQVADLPGNLVSAQKDMTQAANKLTEAANETRPRLW
                     RQALGLILAGAVGAMLVATGQVALNRLVPPSDVQQTADWANAIWNKATPKERELLEQIV
                     NRPAN"
                     /name="hypothetical protein"
     gene            8619..9569
                     /locus_tag="D9T11_RS25770"
     CDS             8619..9569
                     /locus_tag="D9T11_RS25770"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_043849884.1"
                     /translation="MKREGRHLSVKAGGDEAYRVTRQQDGRWLWCDRYGNDGGDNIDLV
                     REIEPGTGYAEAVYRLSGAPTVRQQPRPSEPKRQPPQLPAQGLAAREHGRDYLKGRGIS
                     PDTIEHAEKAGMVRYADGGVLFVGYDRTGTAQNATRRAIAPADPVQKRDLRGSDKIYPP
                     ILPGDPAKVWIVEGGPDALALHDIAKRSGQQPPTVIVSGGANVRSFLERDDVQAILKRA
                     ERVTVAGENEKNPEAQAKADAGHQKQAQRVAEITGREVRQWTPKPEHGKDLADMNARQV
                     AEIERKRQAEIEAERARNRELSRKSRGYDGPSFGR"
                     /name="hypothetical protein"
     ARG             complement(2280..2932)
                     /name="qnrS2"
                     /gene="qnrS2"
                     /gene_name="quinolone resistance pentapeptide repeat
                     protein QnrS2"
                     /tool="amrfinderplus"
                     /drug_class="QUINOLONE"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          2..864
                     /name="IncQ2"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.495"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          7129..9566
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.401"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atggctatca acattatgga ggcgttccag caggaaccgc cacaactgga cttcatctgg
       61 cccggctttc tggctggcac cgtgggcgca ctggtggccc ccggcgcgac cggcaagagc
      121 ttctgggcgc tggaagcggc tatgtccatc gcttgcagcg tggcaggcgg tgacgtggtg
      181 aacctcaagc cgcagcacac cgggcgcgtg gtctatctgg ccggtgaaga tcccgaaccc
      241 gccctgatcc ggcgcgttca cgccatcggc cagcacctca accagtcagc ccgcgaagcc
      301 atcgccgaga acctgaccct tgagccgatc atgggcaagc gcctgaacgt catggacgag
      361 gagcacttgc gccgcgtcat cgagtacagc gccggggcgc ggctgatcgt gctggacacc
      421 ctgagccgga ttcacagcct cgacgagaac agcaacggcg acatggcgcg gcttgtcgcc
      481 acgctggaac acgtcgcggc cagcactggc gcgtctgtcc tgtacctgca ccacgtcagc
      541 aagggcagcg cccgcgaggg gcaaaccgac cagcagcagg cagcgcgggg cgcatcggca
      601 ctgatcgaca acgcccgctg gtgcggcttc gtcgccaaga tgaccgagga cgaggccaag
      661 cgcctgagcg accgcgccta tgaccgtcag cccatcggca acgaccggcg cggcttcttc
      721 gtgcgcttcg gcgtgagcaa acagaactac gacgccaccc cgcttgacca gtggtatgag
      781 cgccgagacg gcggcgtgct ggttccggtg gaactgctgg aagtgccgaa cggcaagaag
      841 gggggcagcc gtgagcaagc atgacctgac ccacgcccgg cacgacccgg cgcattgtct
      901 ggcccccggc ctattccgca gcctcaagcg tggcgagcgc aagcgcggca agctggacgt
      961 ggtgcatgac tatggcgacg gtaagcgcat cgagttcagc ggcccggagc cgctgggcgc
     1021 tgatgacttg cgcatcctgc aaggacttgt ggcaatggct gggccgggtg gccttgtgct
     1081 gtcacctgag ccgcagaccg aggccgggca gcaactccgg ctgttccttg agccgaaatg
     1141 ggaagccttc cagcaggatg cgatggtggt caagggcagc tatcgagcac ttgcccgcga
     1201 aattggctac gccaaccccg acgacactaa gccggttcgg gagtgcatcg agcggctttg
     1261 gaaggtgtcc atcatcgccc agaacggcag gaagcggcag ggcttccgcc tgctgtccga
     1321 gtacgccagc gacgagcagg acggaaagct gtatgtagcc ttgaatcccc tgattgctcg
     1381 cgccgtcatg ggtggcgcgc agcatgtccg catcgacatg gacgaggtgc gggcgctaca
     1441 gaccgacccg gcgcggctga tgcaccagcg cctatgcggc tggatcgacc ccggcaagtc
     1501 cgggcgggtg gaactggaca cgctttgcgg ctacgtctgg ccggggcagg caaccaaccc
     1561 caatacgctc aagacgcgac gccaagcggc tcgcagggcg cttcctgagc ttgccgcatt
     1621 gggctgggca gtcagcgaat acgcaaaaaa caagtttgaa atcggcaggc cgaaagcaac
     1681 gcatctgctc ggcccagcac ccaaaacaca ggaaattagc catgagaaat gattgggaaa
     1741 cgccgccgac agtcgaagtg cgtattccgg tgaagttgaa cagtgattcc ggaatctgtg
     1801 aacaccattt ccggaaagta tgcggacagg gattcttcga gcagaagctg ggctttgagg
     1861 ggaggcatct tttcgactca ctgaacaagc cagcagccaa gcgcagcgcg gcagttgtgg
     1921 atccaagacg ttggaacagg acggttcatc tgacatcaga gtacgtgctc tgaggtgttg
     1981 taggagagga ataaagtttc atactaagca gtcaagtaac cctgtttgta ccgttctaaa
     2041 tgatactcac ccagaaataa agattcatac taattcctac tacaaaatct gagtcacgct
     2101 acaactgagt aataaagtat catactggtt gtcttgagca tccacagatt gattggcccc
     2161 attcaaacac gtttctggac aagaatgagc tagaagtatt gatctagctc tggtgatttg
     2221 aattagaaaa tgtattaatg ttgtgtatag tttatcagca ggtattcgta agcatctagt
     2281 caggaaaaac aacaataccc aacgcttcga gaagctgttc ttgttgccag ctggcaattt
     2341 tgatacctga tgtatcgact tttcgaggat ctaaaccgtc gagttcggcg tgacataaat
     2401 tagcaccctg taggctaaac tgtccccaga catcttcaga aaaaacacct cgacttaagt
     2461 ctgactcttt cagtgatgcg cccgcgaggt gagtccctat ccagcgattt tcaaacagct
     2521 cacatttttc taaacagacc cgctccatat tggcataaga caggttacat ccagtaataa
     2581 aggctgagca aaagtacata cgattactca cttgattggc aaagtttgct cgggaaaagt
     2641 tggccccttt taaatcacac tcacgtaact caataccgta gcaattggcg ttactaaagt
     2701 ttgccatcgc aagctggcat tgttggaaac ttgcatcgcg aaggtctgcg acatcaaaat
     2761 ggcaaccttc gatatcaccc tgttcaatga acttgcagtt aataaatgtc gcatcacgca
     2821 agttagcacg tcgaaaatcg catcggataa aggtgcaagc agtgaaagta atatcactta
     2881 gatcttgatg tgaaaaactg tggtgtcgat atgtgtgacg gtaggtttcc atgttgactc
     2941 ctatgatgaa ctggacgcaa cactaccatc aaagccacat cacatcacca aaataaaaac
     3001 actaaaaatc aagatcttaa aaaccctcat tttgcctatt ttaagggggt aaaatagcgc
     3061 cctgagcctc tatgttaacg ttcaaagtac gcggcaccat tgaaaaaagt tatccgctta
     3121 aaaagccaca gagaaaagga acccaataga ttactgatgt ccgccattag gtaaagtaac
     3181 cttaaacgga agcctgcaat ttgaagtaga tacaaggtct aagaaggaat ggggcataaa
     3241 cgttttagaa atggcagtgt actctgatcg ctgccattta agtatgaatc tttaatactc
     3301 agtatgattc tttattactt agtaacgaaa ctttattcta ctcacacagg tgtccggaat
     3361 cggtgtacac gcaagccgga atcagcgttc attttaaacc ggaataggtg ttcattttcg
     3421 accggaatat gcacgaaggg ctgattgtcc gggcttggat tggcgaggat gggggaaccg
     3481 cagaattcgg aaaaaatcgt acgctaagct aacggtgttc tcgtgacagc tctttgacta
     3541 ggctttctaa ggccattctg atagccatga cttcctgaaa agccatggct aaaatttgtg
     3601 cggctaaaag ggataccgat ggtaaagtaa gttatccctg tcgagatact gaaaagcgtt
     3661 atcctcgttt ttcccaaaac tgttttgcca gttcgctcag agcgctagtt aactgagcga
     3721 cagatttcgc actttgcaaa ttattctgcc cggtctgtac attggtatca gcagcatcgc
     3781 gtatattgat aatactgcgg tttatgtctt cacttactgc tccctgctgc tcgaccgcag
     3841 tcgctatttg cgcgttcatg tcggtaattt cgttaacgcg ttggccaatt ccatcaagag
     3901 ctgtagctgc ttcctctgcg tgagctacac tcgtgtccgc ttgccgacta ctttgctcca
     3961 tgactgtaac agcggattgc gctcgctctt gtagagcgct gatcatgctt tgaatatccg
     4021 ttgtcgattg ctgtgtgcga gcagcaagac tgcgaacctc atcggcgaca acagcaaaac
     4081 cacgcccctg ctcaccagca cgcgcggcct caattgctgc gttgagtgcc aacaaattcg
     4141 tttgctcggc gatccctcgt ataacgtcaa gaacttttga tatctcgtta ctttgacctt
     4201 caagctcatg aataacctga gcggcttgcc taatttcacc ttcaagggca gtgattgact
     4261 ggcttgtgtg ggctaccaga cgctggccag atgccgtctc agtgtctgct cttccggccg
     4321 catctgcagc atgctgtgca ttgctcgcaa cctcttgaat gcttgccgcc atttggttta
     4381 ctgccgttgc tatttgatct gtctctgcct gctgctcaac tgtaagtaca ttgcttgact
     4441 caatatcctt tagtaggcct cgggtgtgtt cgctaagccg atttgatgca tcacctatgc
     4501 gacctactat ggcgcctgtt tcagcttgca tcattcgtaa agcaaactct atttggccaa
     4561 actcatcggt gcgcccagtg tagatggatt gacttaatgg gttattggaa atattcctgg
     4621 ctctttcaac cagtcttcca agaggagaga gaatagccaa aacactaaca gagcttaagc
     4681 ttcctgacat taaagtggct aacaataagc tgcttattga tgtatcagta agcatgccgg
     4741 cagccattgc gctcgatata atactacccc atatgagcaa gagtattttc acggaaaagc
     4801 tagcagccaa tttcggcctc gcggccttcc cgcttctcaa ttgagcatat aatttttccg
     4861 cagccaaaac ctgctcaggt tcaggcttgg tccttacaga ctggtattca acaatcgaac
     4921 cattcttagc tattggcgtt acataagaac ttacccaata gtggtcgcca tttttacagc
     4981 gattttttat tagccccatc catgagcggc cagattttaa tgtactccac atatgctcaa
     5041 atgcagcagg cggcatatct gggtgtctta cgatgttgtg aggctggcct aatagttctt
     5101 cctcagtgaa accactgatt ttaatgaagt caggattaac gtacgtgata tggctttgag
     5161 gggaggtagt cgaaagaata ttggtatctt ttgggagttc taagtttcga cccgtcactg
     5221 gtaaattctg gcgcataaga gcctcaatgg ttggctgttt tattttattg tactcggcat
     5281 taagcccaat ttctttagcg ttacgataag ctagcatggc tacaccgacc ccgctaggct
     5341 gattgggaag cgttgcctga tctactcagg agggcgcttc gataacgtag ccattgagcc
     5401 ggaaaccggc gagcactggg gcagcgtgat ctggtgcgag tatgtgtgac cccctgttta
     5461 actccaccca acccccacgg taactccacc caacccccac ggtaactcca cccaaccccc
     5521 acggtaactc cacccgcccc gatctggaaa acccagacgg ggcgcggatt tgctggcgat
     5581 tcgcaaaatc catccaagat tctctaagaa tctccactag gcgcagcaca gcccgcgccc
     5641 cttcggcgcg gtctggcatt gcctcaaatg cccggccaag accatgcgca aggcgaacac
     5701 ctctcccccc gccttcggcg accccctccg ggggtaaagg gccagcgggg agaggggggg
     5761 gggtgccggt ttcccggcct aaacccctca ggaggcgctt tctggcgttt ctggcgcatc
     5821 cccaaagggc aaccccgcct gacccggcat cggcgcgtct gcggctttct ggggcgcttt
     5881 gcggagcttc gggtagcccg ccttgcgcat ggccttggtc agcgcctcga ttgccgccca
     5941 ggtatccgcc gcccatgcct cggacggctc aaagtccccg gccttgatcg tgtcggcaac
     6001 ctcaacaata cgactggcgg cagacttagc gtaatacact cggcttctct tttctgctgc
     6061 ctgtcgttct gtttcgatgt atgactgtag ttctgttttt tcttcgtctg tcagattctc
     6121 aattaggccg cctgacggct cgtaagaata tacgtcaata gaccccatca tcttgacgat
     6181 ggcgcggcgt ttttccttgt catacccccg gtaggcgagc acctgaaccc ggcgaccttg
     6241 gcgcttaaac tgcatggcgg tttctccgtt tgactgtgtg attatattat agttcgacag
     6301 tcgcacgctg tcgagtgttt attgtcggcc tatttttcaa tccgaccgtc gcactcaaat
     6361 aggtttgcac tgtcggattg agtaacgctc gacagtctgc cgttcttgcg ataccatagt
     6421 tgcattgtat cagttcgccc cggcgccttg cccaccccct ctccccgctg gcccattacc
     6481 gcccttgggc ggtcgggccg agagcgcagc gagcgggccg ggtgggtggc tgttcggtgc
     6541 ttgcatggag atgtgctatt atggtatttg cgcggtggtt tggcatggag caaggtgtaa
     6601 cctacgcttc gcttggttct caccctccgg ccttcggcct acgcccccgc tcccttcggt
     6661 cggggggttg ctgcgcgttt cgcttcgctt cacttactgg agggcttcgc ccatgaacac
     6721 tgacgaatcc aagcgccgcc gccgtgggcc tgcgccgctc gacgccacgg acaagcgcgg
     6781 gcataccgtt tcggtgcgcc tcaacgatgc cgagcttgcg cggctggatt cgcagcgtga
     6841 cgcggtgcag atgcagcggg gcgagtacct acgcgccgcc gctctgcatc gcctgccgcc
     6901 caccatcccg gcagcgaacc gggaacagtg ggcagagttg gcccgcacag cggccaacct
     6961 gaaccagatc gcccggcacc tgaacgaagg gcagcggggc gacggggagc gcatcggcaa
     7021 ggcgctgggc ttgcagttga gcgccgagct tgccaactgc ctgcgattgc tggttctggt
     7081 gcgcggcgac ttgataggcg tcaagcacga ctcggaggac gtggaagatg aaagcgaagg
     7141 ttagccgtgg tggcggtttt cgcggtgcgc tgaactacgt ttttgacgtt ggcaaggaag
     7201 ccacgcacac gaaaaacgcg gaacgtgtcg gcggcaacat ggccgggaat gacccccgcg
     7261 aactgtcgcg ggagttctca gccgtgcgcc agttgcgccc ggacatcggc aagcccgtct
     7321 ggcattgctc gctgtcactg cctcccggtg agcgcctgag cgccgagaag tgggaagccg
     7381 tgacgactga cttcatgcag cgcatgggct ttgaccagac caatacgccg tgggtggcag
     7441 tgcgccacca ggacacggac aaggatcaca tccacatcgt ggccagccgg gtagggctgg
     7501 acgggaaagt ttggctgggc cagtgggaag cccgcagcgc catcgaggcg acccaagagc
     7561 ttgagcatac ccacggcctg accctgacgc cggggctggg cgatgcgcgg gccgagcgcc
     7621 ggaagctgac cgacaaggag atcaacatgg ccgtgagaac gggcgacgaa ccgccgcgcc
     7681 agcgtctgca acggctgctg gatgaggcag tgaaggacaa gccgaccgcg ccagaactgg
     7741 ccgaccggct acatgccgca ggcgtaggcg tccgggcaaa cctcgccagc accgggcgca
     7801 tgaacggctt ttccttcgag gtggccggag tgccgttcaa gggcagcgac ttgggcaagg
     7861 gctacacatg ggcgggacta cagaaatcag gggtgactta tgacgaagct agagaccgtg
     7921 cgggccttga acgattcagg cccacagttg cagatcgtgg agagcgtcag gacgttgcag
     7981 cagtccctga gcctgatgca cgaggacttg aagcgcctac cggtcgcagt ctcgaccgag
     8041 acggcgcaga ccttggaacc gctggcccga ctccaacagg acgtgacgca ggttctggaa
     8101 gcctacgaca aggtgacggc cattcagcgc aagacgctgg acgagctgac gcagcagatg
     8161 agcgcgagcg cggcgaaggc gttcgagcag aaggccgggg agctggacaa gaccatctcc
     8221 gacctgtcca acagcctgtc agggctggaa acgagcatca gcagcatgaa gcagaccgcg
     8281 gagcaggtgg cggacttgcc gggcaatctg gtgagcgcac agaaggacat gacgcaagcc
     8341 gccaacaaac tgaccgaggc agcgaacgag acgcgcccgc gcctttggcg gcaggcgctg
     8401 gggctgattc tggccggggc cgtgggcgcg atgctggtag cgactgggca agtcgcttta
     8461 aacaggctag tgccgccaag cgacgtgcag cagacggcgg attgggccaa cgcgatttgg
     8521 aacaaggcca cgcccaagga gcgcgagttg ctggaacaga tcgtcaatcg gcccgcgaac
     8581 tagatccgac cgcctacctt gaggcgagcg gctacaccgt gaagcgcgaa gggcgacacc
     8641 tgtccgtcaa ggctggcggt gatgaggcgt accgcgtgac ccggcagcag gacgggcgct
     8701 ggctctggtg cgaccgctac ggcaacgacg gcggggacaa tatcgacctt gtgcgcgaga
     8761 tcgaacctgg caccggctac gccgaggccg tctatcggct ttcaggtgcg ccgacagtcc
     8821 ggcagcaacc gcgcccgagc gagccgaaac gccaaccgcc gcagctaccg gcgcaagggc
     8881 tggcagcccg cgagcatggc cgcgactacc tcaagggccg gggcatcagc ccggacacca
     8941 tcgagcacgc cgagaaggcg ggcatggtgc gctatgcaga cggtggcgtg ctgttcgtcg
     9001 gctacgaccg tacaggcacc gcgcagaacg ccacacgccg cgccattgcc cccgctgatc
     9061 cggtgcagaa gcgcgactta cgcggcagcg acaagatcta tccgccgatc ctgccgggcg
     9121 acccggcaaa ggtctggatc gtggaaggtg gcccggatgc gctagccctg cacgacatcg
     9181 ccaagcgcag cggccagcag ccgcccaccg tcatcgtgtc aggcggcgcg aacgtgcgca
     9241 gcttcttgga gcgtgacgac gtgcaagcga tcctgaagcg ggccgagcgc gtcactgtgg
     9301 ccggggaaaa cgaaaagaac cccgaggcgc aggccaaggc cgacgccggg caccagaaac
     9361 aggcgcagcg ggtggccgaa atcaccgggc gcgaggtgcg ccaatggacg ccgaagcccg
     9421 agcatggcaa agacttggcc gacatgaacg cccggcaggt ggcagagatc gagcgcaagc
     9481 gacaggccga gatcgaggct gagagagcgc gaaatcgaga gctttcacgc aagagccggg
     9541 ggtatgatgg ccccagcttc ggcagataag agcgcctgtc agtcggtgtg atgaaccgac
     9601 cgacaggcta accatcatca acctgagtag gaggccgat
//
