LOCUS       NZ_CP032894             3994 bp    DNA     circular CON 27-APR-2024
DEFINITION  Enterobacter kobei strain WCHEK045523 plasmid p2_045523, complete
            sequence.
ACCESSION   NZ_CP032894
VERSION     NZ_CP032894.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN08027255
            Assembly: GCF_003665375.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter kobei
  ORGANISM  Enterobacter kobei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 3994)
  AUTHORS   Wu,W., Long,H., Feng,Y. and Zong,Z.
  TITLE     The complete genome of Enterobacter kobei strain WCHEK045523
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3994)
  AUTHORS   Wu,W., Long,H., Feng,Y. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-OCT-2018) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_003665375.1-RS_2024_04_27
            Annotation Date                   :: 04/27/2024 03:02:54
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,989
            CDSs (total)                      :: 4,870
            Genes (coding)                    :: 4,741
            CDSs (with protein)               :: 4,741
            Genes (RNA)                       :: 119
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 42 of 129
            Pseudo Genes (incomplete)         :: 82 of 129
            Pseudo Genes (internal stop)      :: 32 of 129
            Pseudo Genes (multiple problems)  :: 24 of 129
            Pseudo Genes (short protein)      :: 1 of 129
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP032894.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3994
                     /organism="Enterobacter kobei"
                     /mol_type="genomic DNA"
                     /strain="WCHEK045523"
                     /host="Homo sapiens"
                     /db_xref="taxon:208224"
                     /plasmid="p2_045523"
                     /geo_loc_name="China: Sichuan, Chengdu"
                     /collection_date="2017"
     gene            join(3470..3994,1..330)
                     /locus_tag="D9T11_RS00885"
                     /old_locus_tag="D9T11_00885"
     CDS             join(3470..3994,1..330)
                     /locus_tag="D9T11_RS00885"
                     /old_locus_tag="D9T11_00885"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071579761.1"
                     /translation="MIIQEEHNIDFFKNQMPNKPYCTNNLDMGLSIRNKAKALEMLYLQ
                     ANQPAIQTCLLFDLDKKNSFYTFEQVGLPIPHFITKTPKTGRCHYGYMLKAGVCKTQQA
                     RLKPLKYAAAVEMAMAKKLKADLGFAGLITKNPLNDHWSPFWSGADLYDLDYLADFVDL
                     EKPQIQKKKSEAYGLGRNVNLFEDLRQYAYRTVLNFKKISTFEKFENELLLKAQGLNTF
                     CNPQNPLQYKEIKATVRSVARWTWKNFDSHTFSQIQSNRAKKPRKTNVKNEMLDILMSK
                     GVL"
                     /name="replication initiation protein"
     gene            327..590
                     /locus_tag="D9T11_RS00890"
                     /old_locus_tag="D9T11_00890"
     CDS             327..590
                     /locus_tag="D9T11_RS00890"
                     /old_locus_tag="D9T11_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001722510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_045287009.1"
                     /translation="MIINGKKIKAKEIMEMTGRSERTVRKYFSQSRDDYEKTAMDRRRQ
                     AYELRSQGLKWQQVADKMGCSYHGAVALYRRYVALDMPQNSL"
                     /name="hypothetical protein"
     gene            complement(827..1111)
                     /locus_tag="D9T11_RS00895"
                     /old_locus_tag="D9T11_00895"
     CDS             complement(827..1111)
                     /locus_tag="D9T11_RS00895"
                     /old_locus_tag="D9T11_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="WP_079843899.1"
                     /translation="MSKSTAQQIADLQNKLQALRTKKARQDRREETRQKIILGAEVAKV
                     LGKKPEEINKALILGLLSKINELDEEEKQEFTRLGLNILEEWKNENRKP"
                     /name="conjugal transfer protein TraD"
     gene            complement(1108..1497)
                     /locus_tag="D9T11_RS00900"
                     /old_locus_tag="D9T11_00900"
     CDS             complement(1108..1497)
                     /locus_tag="D9T11_RS00900"
                     /old_locus_tag="D9T11_00900"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_121527125.1"
                     /translation="MNKLITEAEGAKIAGVFKYILEASESILIDHGHVITGQIIKNKKF
                     FADNGHKDFEKLEYYLGSAISSLVDNKVIEGIKDINTDENLVFKKINELSDKEKLILAE
                     CIIKHFEYSNIRGMQKAFNIFGGDK"
                     /name="hypothetical protein"
     gene            1740..2933
                     /gene="mobQ"
                     /locus_tag="D9T11_RS00905"
                     /old_locus_tag="D9T11_00905"
     CDS             1740..2933
                     /gene="mobQ"
                     /locus_tag="D9T11_RS00905"
                     /old_locus_tag="D9T11_00905"
                     /inference="COORDINATES: protein motif:HMM:NF041496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_076997031.1"
                     /translation="MATFHISFKKLRRSEGKSSVYLSAYQNREKTKDNRTGATWDYSKK
                     EGFFGSAILSPAGTPAELVKDSGTLWNAVEAGEKRKDAELCRYLDIAIPKELDDGQKKQ
                     IVLDYCQENFVDYGMIADIAFHDLDSHNPHAHVMLTLRTVSPNGFGNKQREWNNRENIE
                     AWRVNWEIIANDRLKKYGHKSRIDSRSLKDQKEEAERKAVFAKTPKEKAKWISKTIELD
                     RTPMTRIHRHQWRKGQKLRAMEQKEKALLLKIANATYLQQTKPPQPEETMKIEKKPLAR
                     SIIDNLKALYKKTQHIFKSKPNVNKNKKEEKEDPQNFVSSYVINEFGEHILKTELETKP
                     KTGTGTGTGGNNGGGKMGSGSGMSPMNQPQPKIEEPEPEPMPEFEDQQPKRKKKFGM"
                     /name="mobQ"
     Typing          1741..2750
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="85.0"
                     /evalue="0.0"
ORIGIN
        1 tatggtttag gtcgcaatgt taatttgttt gaagatctac ggcaatatgc ttacagaacg
       61 gttttaaatt tcaaaaaaat atccactttc gagaaattcg aaaacgaact actattaaag
      121 gcacagggct taaacacttt ttgcaatcca caaaacccgt tacaatacaa agagataaaa
      181 gcaacagtta gaagtgtagc ccgttggact tggaaaaatt ttgatagcca tacattttca
      241 caaatccaat caaacagagc caaaaaacct cgaaaaacca atgttaaaaa tgaaatgtta
      301 gacatattaa tgagcaaggg ggttttatga tcattaatgg taaaaaaata aaagcaaaag
      361 aaataatgga aatgacaggt cgtagtgaaa gaactgtaag aaaatatttt tctcaatctc
      421 gtgacgatta cgaaaaaaca gcaatggatc gtcgtcgtca ggcttacgaa ctacgaagcc
      481 aggggctgaa atggcaacaa gtagccgaca aaatgggctg ttcgtatcac ggagccgttg
      541 ccctgtatcg ccgttatgtt gccttagata tgcctcaaaa cagcctgtaa cgacttctaa
      601 gcagtttttc cccctaacct aaccaaaccc tccacccctg gggggatttt ttcccctttc
      661 cccgtatccg ggcttgtgtc tggataggtg agggacactc tggtaccaac aaatcaacaa
      721 acccacccag ccaccagagg ccctctggca accttaaaac ctccagccta cggctggcaa
      781 cccttcacaa tgaaaaacca gccaagatcg gctggttttt tattgttcac ggtttacgat
      841 tttcattctt ccactcttct aaaatattta atcctaatcg tgtgaattct tgtttttctt
      901 cttcgtcaag ttcatttatt ttacttaaca acccaagaat taaagccttg ttaatttcct
      961 ctggtttttt acctaaaacc ttagccactt ctgcacccag gattattttt tgtctcgttt
     1021 cttcccttcg gtcctgtctt gctttttttg tccttaatgc ttgtagttta ttttgcaaat
     1081 ctgcgatctg ctgtgcagta gatttactca tttatcaccc ccaaaaatat taaaagcttt
     1141 ttgcattcct ctaatatttg aatattcaaa atgtttaata atacactcag ctaaaattaa
     1201 tttttcctta tcactcaatt catttatttt tttaaaaaca aggttctcat ccgtgttaat
     1261 atctttaata ccttcaatta ctttattatc gacaagagaa ctaattgctg aaccaagata
     1321 atactccaat ttttcaaaat ctttatgccc gttatcagca aagaattttt tgttttttat
     1381 tatttgcccc gtgataacat gcccgtgatc tattaatata ctttcagatg cctctaatat
     1441 gtatttaaaa acacctgcga tttttgcacc ttctgcctct gttataagtt tattcataaa
     1501 acctcctaat tatttatttt gttatttatt acctccatta tttactttag tcaaatatat
     1561 tttagaataa aataaataat atttaatatt gggcgaagcc tggggttcat tacttattat
     1621 tttgaggtga agccgaaaaa ataatattag taatgcccac ttacacgtcg caagcgacgt
     1681 atgggccctg cggggctaaa aaccaaaatt caaaagacaa aaaaagtaag gggtagacta
     1741 tggcgacatt tcatatcagc tttaaaaaat tacgaagatc agaaggtaaa tcttcggttt
     1801 atttatctgc atatcaaaac cgagaaaaga caaaagataa tcgaaccggg gcaacctggg
     1861 attattcaaa aaaagaggga tttttcggct ctgctatcct ctcccctgcc ggcacacctg
     1921 ccgaactggt gaaggactca ggcaccctct ggaatgccgt agaggctggc gagaagcgaa
     1981 aggatgccga attatgccgt tatctggaca tagccatccc caaggagctg gacgacggcc
     2041 agaagaagca gatcgtcctc gattactgcc aagaaaattt cgtggattat ggaatgattg
     2101 cagacatagc atttcatgat cttgatagtc ataaccctca tgctcatgta atgctaactt
     2161 taagaactgt tagcccaaat ggatttggga acaaacaaag agaatggaat aatagagaaa
     2221 acattgaagc ctggcgagtt aactgggaaa taatagcaaa tgaccgatta aaaaaatatg
     2281 gtcataaatc tcgtattgac tcccgttcat taaaagacca aaaagaagag gcagaaagaa
     2341 aggctgtttt tgctaaaact ccgaaagaga aagccaagtg gatttcaaaa actattgaac
     2401 tggacagaac gccaatgacc aggatacaca ggcaccaatg gcgaaaaggt caaaaattaa
     2461 gagctatgga gcaaaaagaa aaagcattat tattaaaaat agctaatgca acatacttac
     2521 agcaaaccaa accacctcag ccagaggaaa ctatgaaaat agagaaaaag cctcttgcaa
     2581 ggtcaatcat tgataattta aaggcattat acaaaaagac ccagcatata tttaaatcaa
     2641 aacctaatgt aaataaaaat aaaaaagaag aaaaagaaga tcctcaaaat ttcgttagta
     2701 gttatgtaat aaatgaattt ggcgaacata tattaaagac agagttagaa acaaaaccta
     2761 aaacaggcac aggaaccgga accggtggaa ataatggagg cggaaaaatg ggttcaggct
     2821 ctggaatgtc tcctatgaac caaccacaac caaaaattga agagcctgag ccggaaccaa
     2881 tgccggaatt cgaagatcag caacctaaga ggaagaagaa atttggaatg tagtatatag
     2941 tggtatatat tcctatatag tagtatcact taatacaact taatattatt taatactata
     3001 ctataagata ctacattttt agagttgtga tcctcgccgg ttctatttcc cctttatgta
     3061 ttgccacagc ttcattaact gacttcataa gcattttaaa aaatttacta tgcttatttc
     3121 ttttccgttt tttctgcact ttcttttttt tcatatattc accaaaaaaa atcgaatcgc
     3181 caatttttct tcgaacaata ctcttttttc tttttttatt gtttttctat cttcggtaat
     3241 gcttcgcacc atagatctga tagtaatgct ttgctgccgt atgtaaagaa ctgccccaac
     3301 ttcgttgggc tgttatctga tatggttaga cgcacacttg acaaattttt gagatcggtt
     3361 agaactggta caggattgaa aaatctataa aaataaaaac cccaccctct gcgattttgt
     3421 tttggcgaca gaaaacagag agtagggcca gataaaaagg actgaaaaaa tgataataca
     3481 agaagaacat aacatcgatt tttttaaaaa tcaaatgcct aacaagcctt attgtacaaa
     3541 taacctcgat atgggattaa gcataaggaa caaggcaaaa gcccttgaaa tgctgtattt
     3601 acaggcaaat cagccagcca ttcaaacttg cttactcttt gatctagata agaaaaattc
     3661 attctataca tttgagcagg tgggcttacc catacctcac tttattacaa aaacaccaaa
     3721 aacaggtcgt tgtcactacg gctatatgct gaaagccggg gtttgtaaga cacaacaagc
     3781 aaggttaaag ccgttgaagt atgccgcagc cgttgaaatg gctatggcga aaaaactaaa
     3841 agcagatctg gggtttgctg gattgattac aaaaaaccca ctcaatgatc actggtcgcc
     3901 gttctggtca ggtgctgacc tttatgacct ggattattta gctgattttg tggacttaga
     3961 aaagcctcaa atacaaaaga aaaaaagcga ggct
//
