LOCUS       NZ_CP032887             1902 bp    DNA     circular CON 17-JUN-2024
DEFINITION  Escherichia coli strain SCEC020022 plasmid p8_020022, complete
            sequence.
ACCESSION   NZ_CP032887
VERSION     NZ_CP032887.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN06909177
            Assembly: GCF_002165095.2
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1902)
  AUTHORS   Liu,L., Long,H., Feng,Y. and Zong,Z.
  TITLE     The complete genome of Escherichia coli strain SCEC020022
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1902)
  AUTHORS   Liu,L., Long,H., Feng,Y. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-OCT-2018) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
REFERENCE   3  (bases 1 to 1902)
  AUTHORS   Liu,L., Feng,Y. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAY-2017) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002165095.2-RS_2024_06_17
            Annotation Date                   :: 06/17/2024 07:33:24
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,250
            CDSs (total)                      :: 5,130
            Genes (coding)                    :: 4,897
            CDSs (with protein)               :: 4,897
            Genes (RNA)                       :: 120
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 233
            CDSs (without protein)            :: 233
            Pseudo Genes (ambiguous residues) :: 0 of 233
            Pseudo Genes (frameshifted)       :: 86 of 233
            Pseudo Genes (incomplete)         :: 158 of 233
            Pseudo Genes (internal stop)      :: 40 of 233
            Pseudo Genes (multiple problems)  :: 46 of 233
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP032887.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1902
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="SCEC020022"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="p8_020022"
                     /geo_loc_name="China: Sichuan"
                     /collection_date="2016-10"
     gene            236..376
                     /locus_tag="C7V31_RS00815"
                     /old_locus_tag="C7V31_00815"
     CDS             236..376
                     /locus_tag="C7V31_RS00815"
                     /old_locus_tag="C7V31_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001380222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="WP_205589770.1"
                     /translation="MRKCSMWLPVLFRFLVDSLATLGHRLAASGITSRMLSVIPLLFFQ
                     G"
                     /name="replication protein"
     gene            complement(826..1113)
                     /locus_tag="C7V31_RS00820"
                     /old_locus_tag="C7V31_00820"
     CDS             complement(826..1113)
                     /locus_tag="C7V31_RS00820"
                     /old_locus_tag="C7V31_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_049092722.1"
                     /translation="MRIKYESRWRLEELKLEICSSIDELINKYGIEYATGINLYMNCED
                     KEFNLVDVINEDGNKIMFVVSNDEKIQKPKKIAKKKTPAPVIELDKYRKK"
                     /name="hypothetical protein"
     gene            complement(1120..1659)
                     /locus_tag="C7V31_RS00825"
                     /old_locus_tag="C7V31_00825"
     CDS             complement(1120..1659)
                     /locus_tag="C7V31_RS00825"
                     /old_locus_tag="C7V31_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000893324.1"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA];
                     GO:0006276 - plasmid maintenance [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="WP_000893325.1"
                     /translation="MLEQQFEDFEQQKRRDHQVQDQSKNAKQKRDRLNPPDWSQVTKAG
                     WNMLDAISEKVPRAFRLYSFFAQNIDKSCGAVVCDQKFLADYFGVSLRTIQYWIGDLES
                     IGAIVKIPVGIYYAYALDPYQVWRGYNNTKKYAAFNAKTLTQKDGVIDRKLRLMLSRRE
                     EFESSLKYSNHDSEQG"
                     /name="replication/maintenance protein RepL"
     Typing          330..590
                     /name="Col(MG828)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.618"
                     /coverage="100.0"
                     /evalue="8.2e-133"
ORIGIN
        1 cccagtcacg tagcgatagc ggagtgtata ctggcttact cctctgcaag ccttaccagc
       61 cgtgattttt gccagatttc atgctctcaa cgttcaacaa ctccatcatg gttttgccgt
      121 ccgttcaccg ctaaaaagcc tcagaaatcg ggacaggatg tgcatttgtc ggaccgcgca
      181 cacgcaatca gacaaatgcc acctggtcag ggcttcaccc cgacaccccg catcagtgcg
      241 taagtgctca atgtggcttc cggttctctt ccgcttcctc gtcgattcgc tcgctacgct
      301 cggtcatcga cttgcggcga gcggtatcac ctctcgcatg ttgtcggtga tcccgttgtt
      361 gttctttcag ggatgagtcg tcagatagcc cttcaaacgc gctcagaagc tctcagacgc
      421 actaaacggg tcttaaatgc ccgtcagggc atgggaggcg cgaaaaacgc ttacagagcg
      481 tttcaggagg tcaggcgcgc cgatacccct aaccaggggc tttcaggtcg ctaggaacag
      541 cgtcagcccc ctgcaaagcc tatttgcacg gcacaggcag ctattcggtt ggtaggtggt
      601 cgtagaccat tggtgttaca gcgatggggg tctggggttc tccccagacg gtttccggtt
      661 gcgctcaagg cgctatcgga accgtggcgg ctcggtcgtt ctggagagcg gggaacaaaa
      721 acacgcaagg gattcgaggc ggcaaaagag caatgcagga tagacaatca tggtcagaac
      781 gttaacacgt agcccctgat tgtccgcctg ccgctggctc aatggtcatt tttttcgata
      841 tttatctagc tcaattacag gtgctggagt ttttttcttt gctatttttt ttggcttttg
      901 tattttttca tcgttgctaa cgacaaacat tattttattt ccatcttcat tgataacatc
      961 aacaagatta aattctttat cttcacaatt catataaaga tttattcctg ttgcatattc
     1021 aataccatat ttattaatta actcatctat tgaactgcat atttctaatt ttagttcttc
     1081 taatctccaa cgtgattcat atttaattct catagtgcat cacccctgct ctgagtcatg
     1141 attagagtac tttaaggaac tttcaaactc ttcacgccta gaaagcataa gccgcaattt
     1201 acgatctata accccatctt tttgagttaa tgtttttgcg ttaaatgctg catatttttt
     1261 agtattgtta tatccacgcc atacctgata aggatctaat gcataggcat aatatatgcc
     1321 tactggtatt ttaactattg cgccaatact ttctaaatcc ccaatccaat attggatcgt
     1381 tcttaaacta acaccgaaat aatcagctaa gaatttttga tcacatacaa cagccccaca
     1441 tgatttatct atattctggg caaaaaaaga atataaccga aatgctctag gaactttttc
     1501 tgatatagcg tccaacatat tccaaccagc cttagtaacc tgcgaccagt ctggaggatt
     1561 caaccgatct cgtttttgct tagcgttctt ggattgatct tgtacctgat gatctcgccg
     1621 tttttgctgt tcaaagtctt caaattgctg ttccaacata cttgcatcct tacctgcaat
     1681 gatatttcgc accttacgaa atttatatcg tacagtacga aacaacacat gcaaacatgt
     1741 ttgcgtatac acatgcaaat aacgtttcgt atgatggtgt cttaagtcaa tgatttttaa
     1801 agaaatgtca atttttgctc taaagatcta tcaaagcgtt tttttactgc tcatgattta
     1861 acactaccta actgcttttg gggtgtcggg gcgcagccct ga
//
