LOCUS       NZ_CP032886             2790 bp    DNA     circular CON 17-JUN-2024
DEFINITION  Escherichia coli strain SCEC020022 plasmid p7_020022, complete
            sequence.
ACCESSION   NZ_CP032886
VERSION     NZ_CP032886.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN06909177
            Assembly: GCF_002165095.2
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2790)
  AUTHORS   Liu,L., Long,H., Feng,Y. and Zong,Z.
  TITLE     The complete genome of Escherichia coli strain SCEC020022
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2790)
  AUTHORS   Liu,L., Long,H., Feng,Y. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-OCT-2018) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
REFERENCE   3  (bases 1 to 2790)
  AUTHORS   Liu,L., Feng,Y. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAY-2017) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002165095.2-RS_2024_06_17
            Annotation Date                   :: 06/17/2024 07:33:24
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,250
            CDSs (total)                      :: 5,130
            Genes (coding)                    :: 4,897
            CDSs (with protein)               :: 4,897
            Genes (RNA)                       :: 120
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 233
            CDSs (without protein)            :: 233
            Pseudo Genes (ambiguous residues) :: 0 of 233
            Pseudo Genes (frameshifted)       :: 86 of 233
            Pseudo Genes (incomplete)         :: 158 of 233
            Pseudo Genes (internal stop)      :: 40 of 233
            Pseudo Genes (multiple problems)  :: 46 of 233
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP032886.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2790
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="SCEC020022"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="p7_020022"
                     /geo_loc_name="China: Sichuan"
                     /collection_date="2016-10"
     gene            complement(1670..2659)
                     /locus_tag="C7V31_RS00810"
                     /old_locus_tag="C7V31_00810"
     CDS             complement(1670..2659)
                     /locus_tag="C7V31_RS00810"
                     /old_locus_tag="C7V31_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_087526068.1"
                     /translation="MENPELQNLADYSPADKTWDEHRSISDDVGGIYLRAAGFERYGER
                     VEGCSGILRFGWSTNEETGETRLRLRDARFCRVRHCPICQWRRSLMWQARFYQSLPKIV
                     AEYPDARWLFLTLTVRNCPITELGDTLTVMNAAFQRLKDRKEFRGVLGWVRTTEVTRGK
                     DGSAHPHFHTLLMVSPSMLSGDGYVKHARWVELWRDCLRVEYDPNVDIRVVKSRKPKDG
                     ESLPCSVVDQLRGVVVETLKYSTKPADMLVDPEWFLELTRQVHKRRFVATGGVLKDVLQ
                     LERETNEDLIMADGMAEGADDGSRLAFSWRAVAKKYRRDRTMDKPKQK"
                     /name="protein rep"
     Typing          438..522
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="93.023"
                     /coverage="93.0"
                     /evalue="3.4000000000000004e-27"
     Typing          complement(1384..1463)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="92.683"
                     /coverage="93.0"
                     /evalue="2.04e-24"
     Typing          1753..1944
                     /name="Col(MP18)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.083"
                     /coverage="100.0"
                     /evalue="6.45e-58"
ORIGIN
        1 ttttttgacc gctaaaacca aaatacactc aaaaccattt cggagcaaat tgccgttcag
       61 cccagtcctg acaaggccta gaggcaaatt tgaatcccag agttatccct taagtataaa
      121 tacaaggatc gcgcgcgaga tttgcttgaa ttttgagcgg gattttcagt aacccaaaac
      181 ctcccaaccc cacccactta cacccatctt acccacagac tttcaccccg attttgtgaa
      241 caacaaaaaa ccgtcttgca ggtggttggc ctcactccgt gacaccaacc accatcccca
      301 gcgttttccc agtgccgtcg tcaatcacaa aatcggaacc cacgccagag gcgcggggcc
      361 ccgcgatttt ctgtttgacg agttcgggaa cggtgagcgg tacgcggaat tttttcgcgg
      421 gccgtgaggc gttggcgggg tgtcggggcg cagccctgac caggtggtaa ttgtgatagc
      481 gtcgcgtgtg acggtattac aattacacat cctgtcctgt ttcaactgat aaataatccc
      541 tgtgcagtgc agccgctgtt gcataaaatt tcgctctgcg tcgagtgttt cttaatcaat
      601 aatcccgctg atactccgga aaaacgcgag tctgttctca tgcctgaaat gtccacacac
      661 cacgcaaaac taactttttg ctgatttctt ttttaaatca ggttgttatg tttaaaaaaa
      721 tgtattttct tattcagatt tatacaaaag cgcgtcgtcg acgaccctat ggcgacccta
      781 ggacgaccct acctagggtc agtactgact atctgtagaa aaaacagtta attaatgtag
      841 gggcggccct aggggcgtgt cgcatttttt tacagagcga tcctaggggc gcccctagga
      901 tcgctctggt ctgatatgca gcaatgagcc agggattcac gaattgaaaa tgtccgaaaa
      961 tgtgtcttat aggcactgta tgcgcccaaa ccgatcgcca ttcagtttca tgcatagtca
     1021 tgcaacgcgc tgaaaacgtt cctgacgcat ttttccggtt taccctgggg aaaacatccc
     1081 tttttgcggt gttcgcggca gaatcgcgtt cagcgcgttt tagcggtgcg cgtaacaagt
     1141 ccttacacca cgcgccccac cccgcggtcg ttcccgttcc gtacagccat cgccaccgac
     1201 gcgccaaatt ctggcgaccg gacagcaaat cattttgccg tttttgtgcc gcagccgttc
     1261 cgcgtgatcg acaaactggc agtcggggaa tctcacgaag tgcgcactta tacaaacttc
     1321 acttcgtttc gtttgtcgtg cggtctgcga cgctcaaaaa accgagaaac ggcgggaatt
     1381 ttcgggacag gatgtgtaat tgtaataccg tcacacgcga cgctatcaca attaccacct
     1441 ggtcagggct gcgcccgaca ccctcgacac cgtgccaaat gcacgttaaa aaaacgggcg
     1501 cgtttctggt gtcggaatgc aactaagggg ttaggtttgt taggggatgc cagaagcgag
     1561 ctaaaacgcc acctgacgca ttaaactggc tcagggtata cctgagcgcc accgaaacct
     1621 aaaacgcgcc tgagcgcatt ttagaggcct taaaatcgat ttttagatgt tatttctgtt
     1681 ttggcttatc cattgtgcga tcgcggcgat actttttagc caccgcccgc catgaaaacg
     1741 ccagacgcga tccgtcatcc gcaccctctg ccatgccatc tgccataatc aaatcctcgt
     1801 tcgtttcccg ctccagttgc aaaacgtcct taagcacacc acccgtcgcc acaaaacggc
     1861 gcttgtgtac ctggcgtgtt aactctagga accattcagg atcgaccagc atgtcggcgg
     1921 gctttgtaga atatttcagt gtttcaacca ccaccccgcg cagttgatcc accacagaac
     1981 agggcaacga ttcaccgtct ttcggttttc tggacttcac aaccctgatg tcgacgttcg
     2041 ggtcatactc cacccgcaga cagtcgcgcc acaactccac ccatcgggca tgtttcacat
     2101 aaccatcacc ggacaacatc gagggggaaa ccatcagcag ggtgtggaaa tggggatgag
     2161 cgctgccgtc cttgccgcgg gtgacttccg tcgtgcgcac ccagcccagc acgccgcgaa
     2221 actctttgcg atctttcagg cgctggaagg ccgcattcat cacggtcagg gtgtcgccaa
     2281 gctccgtaat cggacagtta cggacggtca gggtcaggaa cagccagcgt gcatcaggat
     2341 attccgcaac gatctttggc agggactgat agaaacgggc ttgccacatc agggaacgcc
     2401 tccactgaca gatcggacag tgccggaccc gacagaaccg cgcatcacgt aaccgcaggc
     2461 gcgtttctcc ggtttcctcg ttggtggacc acccaaaacg cagaatgccg ctacaaccct
     2521 ccacgcgttc gccgtagcgt tcaaatccgg cagcccgcag ataaattccg cccacatcat
     2581 cggaaatact ccggtgctca tcccacgttt tgtccgcagg ggagtaatcc gccagattct
     2641 gaagttcagg gttttccatg cctgccgctc cctagtgcgc gacaggcgaa attacgtgtt
     2701 atcattccaa tatcacctgt atatcgagtt atcaatccaa tttcccaggt ggtcatcccc
     2761 ccgaacgacg cccatcgttg cgggggttta
//
