LOCUS       NZ_CP032885             4234 bp    DNA     circular CON 17-JUN-2024
DEFINITION  Escherichia coli strain SCEC020022 plasmid p6_020022, complete
            sequence.
ACCESSION   NZ_CP032885
VERSION     NZ_CP032885.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN06909177
            Assembly: GCF_002165095.2
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4234)
  AUTHORS   Liu,L., Long,H., Feng,Y. and Zong,Z.
  TITLE     The complete genome of Escherichia coli strain SCEC020022
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4234)
  AUTHORS   Liu,L., Long,H., Feng,Y. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-OCT-2018) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
REFERENCE   3  (bases 1 to 4234)
  AUTHORS   Liu,L., Feng,Y. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAY-2017) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002165095.2-RS_2024_06_17
            Annotation Date                   :: 06/17/2024 07:33:24
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,250
            CDSs (total)                      :: 5,130
            Genes (coding)                    :: 4,897
            CDSs (with protein)               :: 4,897
            Genes (RNA)                       :: 120
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 233
            CDSs (without protein)            :: 233
            Pseudo Genes (ambiguous residues) :: 0 of 233
            Pseudo Genes (frameshifted)       :: 86 of 233
            Pseudo Genes (incomplete)         :: 158 of 233
            Pseudo Genes (internal stop)      :: 40 of 233
            Pseudo Genes (multiple problems)  :: 46 of 233
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP032885.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4234
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="SCEC020022"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="p6_020022"
                     /geo_loc_name="China: Sichuan"
                     /collection_date="2016-10"
     gene            complement(join(3706..4234,1..380))
                     /locus_tag="C7V31_RS00775"
                     /old_locus_tag="C7V31_00775"
     CDS             complement(join(3706..4234,1..380))
                     /locus_tag="C7V31_RS00775"
                     /old_locus_tag="C7V31_00775"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_058657849.1"
                     /translation="MSAALQYFEENLPHRPYHTDDPAFGLRISGKGRALLARYIQQNQP
                     HAQHWLVFDVDREGAAIDWSDRNAPAPNITVKNPVNGHAHLLYALNLAVRTAPDASVKV
                     LKYAAAIERALCEKLDADVNYSGLICKNPFHLEWQVMEWREEAYTLDELADYLDLSASE
                     RRSVDKHYGLGRNCHLFEITRKWAYRAIRQGWPAFSQWLDAVIQRVEMYNASLPVPLSL
                     AECRAIGKSIAKYTHRKFSPEGFSAVQAARGRKGGLAKGEAYEDKRFMALCMLENGYSQ
                     KAIAAMLDVHRNTIRNWAMHK"
                     /name="replication initiation protein"
     gene            complement(827..3046)
                     /gene="mobQ"
                     /locus_tag="C7V31_RS00785"
                     /old_locus_tag="C7V31_00785"
     CDS             complement(827..3046)
                     /gene="mobQ"
                     /locus_tag="C7V31_RS00785"
                     /old_locus_tag="C7V31_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_063106751.1"
                     /translation="MAIYHLSMKIISRKNGYSAVASAAYRSGSVIPDDRTGLIHDYTRK
                     RGVDDAVILTPANAPSWCGDRSVLWNAVEKAEQRRNSQLAREIELAIPREISREAAREA
                     VLAFVRENFVSRGMIADVAFHHMDRTNPHAHIMLTTRAVGETGFAGKVRDWNDRALAET
                     WRASWADHANRALANAGYQEEIDHRSYERQGLEKAPGLHLGKAACAMEKRGMETERGEQ
                     NRLINSLNLEIQVSRTRLAVIQVQENVRKRELSDAARRAAEALNLTIPAANASADTLRE
                     FIATLPQECGNAWEMTPEFVAMNGKANDIKREGNALVKERDILEKEMTGLRKARPVASI
                     LSELPLMTWAEPEYRKRQLRFWKLEKQIESLLRTYRAVKERDIPVRRQAFETQWNTWIS
                     PGMAELKEKLSAREAERRREEAEAEARRKEQEYDARLKHHDNHRLSRETALAGVITELG
                     RAREPGTGRITRYMMLSNRAGEFTVWGDELAHYPQSVHDPVNVYLSPGGAVMVSDIREG
                     MPESHETMARPERVRMYSGATVRHVLEQMRQGWPSYGFPALPHHWPDNFYFSDDRRPAA
                     SPLPSAHRVDVTAYAAPEQLMPVVFSTERNSRTLNLLLCKGPEEMLVGFVRLEDELRPV
                     LALPSPDYSHLMVSIITENGIHLAGYGEAINRDADTPYPPEPKLMQFRLKGCQDTLFAA
                     INKPEEMPDYLFRQLGFNQTWHEWKRDEQHRQQQRRPGHYRGMSM"
                     /name="mobQ"
     gene            3225..3500
                     /locus_tag="C7V31_RS00790"
                     /old_locus_tag="C7V31_00790"
     CDS             3225..3500
                     /locus_tag="C7V31_RS00790"
                     /old_locus_tag="C7V31_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_052995416.1"
                     /translation="MSRTLEQKIAEAEARLQRLKAKSRSLDTAQKVIVGAAMLARVRRP
                     EEAQLRAFLLQFLRKEVTRQADVNRLQPLINELEKLPRPPAKPQNG"
                     /name="hypothetical protein"
     Typing          2689..3047
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          3048..3218
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="96.512"
                     /coverage="97.0"
                     /evalue="1.23e-74"
     Typing          complement(2..380)
                     /name="Col156"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="91.842"
                     /coverage="90.0"
                     /evalue="8.710000000000002e-148"
     Typing          complement(3707..4194)
                     /name="rep_cluster_2361"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(831..3046)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.099"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ccgctgtagt tcacatctgc gtcaagtttc tcacacagcg cacgttcaat tgcagcggcg
       61 tatttcagca ccttaaccga cgcatcaggc gcggttctca cggcaagatt gagcgcgtat
      121 agcaggtgag cgtgcccatt cacgggattt tttacggtga tgttcggcgc gggggcgttc
      181 cggtcgctcc agtcaatcgc agccccctcg cggtcaacgt caaaaaccag ccagtgttgc
      241 gcatgtggct ggttctgctg aatgtaccgc gcaagaagcg cacgcccttt gccggagatg
      301 cgaaggccga aagcgggatc atccgtgtga tagggacggt gaggtaaatt ttcttcgaag
      361 tattgaagcg cggcgctcat gtgttacgat tccttccagg ttatcgaccg acatcgcccc
      421 tgtatcttcg tgaaagtctt ggcggatcta cacgaatttc aggggcgttt gtttttcagt
      481 cgcttacact acacccaacg gcaaatcctg tcgatccttc ccgaaaaacc gatccattta
      541 ccataactcc tgttgtacgt accccttaaa acgctctcag acgcgttctg acgcaaaaaa
      601 cacaaaaaga caagaaaaca ccaggccaaa ccagaaaaac gcgtcagaat cgttttcagg
      661 ggtctgcata actattcacg aaagcgatgc gcgatcggtt tcgaccgttc agaagcgcga
      721 acgggtcaaa acggcaaact ttctcatcca gaaaacgcct cacgggacgc ggaatttttt
      781 cgtgtcccgt gagacgttgg cggggttcgg ggtgcagccc cggatatcac atactcatgc
      841 cccggtaatg tccggggcgt cgttgttgtt gcctgtgctg ttcgtcccgc ttccattcat
      901 gccaggtctg attaaatccg agttgacgga agagataatc cggcatctct tccggtttgt
      961 tgatggcagc aaaaagcgtg tcctgacagc ccttaagcct gaactgcatc agcttcggtt
     1021 ccggtgggta cggagtatca gcatcacggt ttatggcctc accgtagcct gccaggtgta
     1081 tcccgttctc tgtgatgatg cttaccatca gatgactgta atccggcgac ggaagcgcaa
     1141 gaacgggacg cagctcgtcc tccaggcgca caaaaccgac aagcatttcc tccggccctt
     1201 tgcacaacag cagattcagc gtcctgctgt ttcgctctgt cgaaaagaca acgggcatga
     1261 gttgctccgg tgccgcataa gcggtgacgt ccacccggtg cgctgacggc agcggagagg
     1321 ctgcgggcct gcggtcgtcg ctgaaataaa aattatccgg ccagtgatgc ggcagcgccg
     1381 gaaaaccgta agagggccac ccctggcgca tctgttccag tacatgccgg accgtcgcac
     1441 cggaatacat tctcacacgc tcaggccgcg ccatcgtctc atgagattct ggcattccct
     1501 cacgtatatc cgagaccatc acagccccgc ctggcgacag gtaaacattc accgggtcat
     1561 gaacactctg ggggtaatgc gccagctcat caccccatac cgtgaattct ccggctctgt
     1621 tactcagcat catgtagcgg gttatcctgc ccgttcccgg ctctctggca cgccccagct
     1681 ccgtaataac cccggctaat gccgtttctc ggctcaggcg atggttatca tggtgtttca
     1741 gccgtgcgtc atattcctgt tccttccggc gcgcttccgc ttcggcttcc tccctgcgcc
     1801 gctccgcttc ccgtgctgac agtttttctt tcagctctgc cattcccggc gaaatccacg
     1861 tattccactg cgtttcaaag gcctgacgac ggacgggaat gtcccgttct ttcacggccc
     1921 tgtaggtgcg gagaagagat tcaatctgtt tctcgagttt ccagaagcgg agttgtcttt
     1981 tgcggtattc cggttcagcc catgtcatca ggggaagctc tgacaggatg gacgctaccg
     2041 gacgggcttt tctgagtccg gtcatctctt tttcgagaat atcccgctct ttcaccagcg
     2101 cattcccctc acgtttgatg tcgtttgcct tcccgttcat cgccacgaac tccggtgtca
     2161 tctcccatgc gttcccgcat tcctgcggca gtgtggcaat gaattcccgc agggtatccg
     2221 ctgaggcatt cgcggcggga atggtcagat taagggcttc cgctgcacga cgtgcggcat
     2281 cgctgagttc ccgtttccgc acgttctcct gtacctggat gactgccagc cttgtgcggg
     2341 aaacctgtat ttccaggtta aggctgttaa tcaggcggtt ctgctcaccg cgttctgttt
     2401 ccatgccccg tttttccatc gcacaggcag cctttccgag gtgaaggccc ggcgctttct
     2461 ccagtccctg acgctcgtat gaacgatggt ctatctcttc ctggtagccg gcgttcgcaa
     2521 gcgctctgtt cgcatggtca gcccatgatg cgcgccacgt ctcggcgagt gcccggtcgt
     2581 tccagtccct gacctttcct gcgaatcccg tttccccgac agctctcgtg gtcagcatga
     2641 tgtgtgcatg ggggttggtc cggtccatat gatggaacgc cacatcggca atcatgcccc
     2701 gactgacaaa gttttcccgg acaaaagcga gaacggcctc ccgtgcggcc tcgcgggaaa
     2761 tctcacgggg aatggcgagt tcaatctccc ttgccagctg ggagttccgg cgctgttcgg
     2821 ctttctcgac cgcattccag agaacggaac ggtcaccaca ccaggacggt gcattcgcag
     2881 gggtgagaat gaccgcatca tcaacgccgc gtttacgggt gtaatcgtgg attaatccgg
     2941 tacggtcatc gggtatgaca gagccggaac ggtaggcagc agaagcaacg gcactgtagc
     3001 cgttttttcg cgaaatgatt ttcatgctga gatgataaat cgccatttct gccgtaatgc
     3061 ctttttgccg ttttctttca gccccgcagg gcgcacacaa tcgaaacgaa gtgaagattg
     3121 tataagtgcg cacttcgtga tatgccgtgc attatgccag ctcagttttt aaactaccag
     3181 tgacgagcta aaatgcgatt tttcaccgcg caaggagtca cgaaatgagc agaacactcg
     3241 aacagaagat tgctgaagct gaggccagat tgcagcgcct gaaggcgaag agccggagcc
     3301 tggacactgc gcaaaaagtc attgtgggag cagccatgct ggcgagggta cggaggccgg
     3361 aagaggcgca actgcgtgcg ttcctgctcc agttcctgcg aaaggaagtc acaagacagg
     3421 ccgatgtgaa ccggctacaa ccactgatta acgagctgga gaagctacca agaccacctg
     3481 cgaaaccgca gaacggataa gccccggcgc gtaagtgcca catgcaccac cggacgcctt
     3541 tgcgcttcct cgcacattcg ctcgctacgc tcggtcatga ggctgcggcg agcgctacca
     3601 ccgcaatcac gaggaaacca gcgccattgc tgggcggaac cggaaaaacc gcaggaacgc
     3661 caacagctca aaaaagcgcc gaaaaggcgc tgttatctga taaggctatt tgtgcattgc
     3721 ccaattacgg atcgtatttc gatgtacatc cagcatcgcc gcaatagctt tctgagaata
     3781 tccattctcc agcatacata gcgccatgaa acgcttgtcc tcgtaggctt cgcctttagc
     3841 aaggccacct ttccggcctc gagcggcctg tacagcggaa aagccttccg gtgagaattt
     3901 tctgtgcgtg tatttcgcaa tgctcttgcc aatagcccgg cattcagcca gtgaaagagg
     3961 aacgggaagc gatgcgttgt acatttcgac acgctggatc acggcatcaa gccactgtga
     4021 gaatgctggc catccctgac gaatcgctct gtaagcccat ttacgcgtta tttcgaacag
     4081 gtggcagttt cgccccagcc cgtaatgttt atcgacgcta cgacgctctg aggcgctcaa
     4141 atcgagataa tcagccagtt catcgagggt ataggcttcc tcgcgccact ccatcacctg
     4201 ccattccagg tggaacggat ttttgcaaat cagt
//
