LOCUS       NZ_CP032884             4658 bp    DNA     circular CON 17-JUN-2024
DEFINITION  Escherichia coli strain SCEC020022 plasmid p5_020022, complete
            sequence.
ACCESSION   NZ_CP032884
VERSION     NZ_CP032884.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN06909177
            Assembly: GCF_002165095.2
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4658)
  AUTHORS   Liu,L., Long,H., Feng,Y. and Zong,Z.
  TITLE     The complete genome of Escherichia coli strain SCEC020022
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4658)
  AUTHORS   Liu,L., Long,H., Feng,Y. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-OCT-2018) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
REFERENCE   3  (bases 1 to 4658)
  AUTHORS   Liu,L., Feng,Y. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAY-2017) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002165095.2-RS_2024_06_17
            Annotation Date                   :: 06/17/2024 07:33:24
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,250
            CDSs (total)                      :: 5,130
            Genes (coding)                    :: 4,897
            CDSs (with protein)               :: 4,897
            Genes (RNA)                       :: 120
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 233
            CDSs (without protein)            :: 233
            Pseudo Genes (ambiguous residues) :: 0 of 233
            Pseudo Genes (frameshifted)       :: 86 of 233
            Pseudo Genes (incomplete)         :: 158 of 233
            Pseudo Genes (internal stop)      :: 40 of 233
            Pseudo Genes (multiple problems)  :: 46 of 233
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP032884.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4658
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="SCEC020022"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="p5_020022"
                     /geo_loc_name="China: Sichuan"
                     /collection_date="2016-10"
     gene            complement(join(4612..4658,1..169))
                     /locus_tag="C7V31_RS27170"
     CDS             complement(join(4612..4658,1..169))
                     /locus_tag="C7V31_RS27170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137421.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWENAFAEWRTMSGLMQRENA
                     ALSERVTGLSRQVQSLSEQLRRLSQG"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(173..658)
                     /locus_tag="C7V31_RS27175"
     CDS             complement(173..658)
                     /locus_tag="C7V31_RS27175"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_001086854.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESE
                     KRISAAILDHDRKLSAAMSQRTKGMLRMVSQTWLTIVLVSTLLTASGASILWWQGQQIL
                     DNYTTIREQKRTQAMLSERNSGVQLSTCGEQRRRCVRVNPEAGRFGEDSSWMILAGK"
                     /name="MbeB family mobilization protein"
     gene            complement(<693..1397)
                     /locus_tag="C7V31_RS28230"
                     /pseudo=""
     CDS             complement(<693..1397)
                     /locus_tag="C7V31_RS28230"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(1336..1659)
                     /locus_tag="C7V31_RS00750"
                     /old_locus_tag="C7V31_00750"
     CDS             complement(1336..1659)
                     /locus_tag="C7V31_RS00750"
                     /old_locus_tag="C7V31_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_001347450.1"
                     /translation="MLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPARAGKLPSL
                     SPALLRQLAGMGNNLNQIARQVNAGGGSGHDRVQVVAALMAIDAGLERLRHAVLEKGAD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            complement(2906..3385)
                     /locus_tag="C7V31_RS00765"
                     /old_locus_tag="C7V31_00765"
     CDS             complement(2906..3385)
                     /locus_tag="C7V31_RS00765"
                     /old_locus_tag="C7V31_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001050742.1"
                     /translation="MNLLLPRDIVEAVLNDKKTKNARVAKCDGSEFFLELPSMNADFPA
                     GKIILKLGDSGFYNKRTKSLEGAYGLRHIWDKHRVEIGATSAEDIVIFLESILLAGAEV
                     LIDPKKGQNKAIVVESGTGMMILELKKPNGEDPYYSIITAYDRKSHPGTKLHTLI"
                     /name="hypothetical protein"
     gene            3862..4413
                     /locus_tag="C7V31_RS00770"
                     /old_locus_tag="C7V31_00770"
     CDS             3862..4413
                     /locus_tag="C7V31_RS00770"
                     /old_locus_tag="C7V31_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000654246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000654246.1"
                     /translation="MKDMKAVVVFTGKDLNIMRTEGGSGYWHARTDRLNDADYLIAVRN
                     RRETWAVKDLEHGTAFLIAKITGCFKSPDYDDRNVITFDEYAEIHTPKAWKMLTDGQRY
                     PVAYLSAQEAFLRIGVTPEQLEWKKFHPSSPSVPNTVIPGLAEEKTEKLSLNEAIERAK
                     KDISNATGIDSSAITISIKI"
                     /name="hypothetical protein"
     Typing          complement(1761..1947)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="85.263"
                     /coverage="87.0"
                     /evalue="2.7100000000000003e-47"
     Typing          1927..2406
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.938"
                     /coverage="93.0"
                     /evalue="3.51e-174"
     Typing          complement(4..1346)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.411"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 gcctgctcaa gcccgtgacg cgctcgctca gcgccgcgtt ctcccgttgc ataagaccag
       61 acatcgtccg ccattccgcg aaggcgttct cccactcgtc cagccttttc gagtagtcct
      121 gctgtagctg ctctaatgcg ctcagcaact gtttttccag ctccgtcatg tgctatttcc
      181 ccgccagtat catccagctc gaatcctctc cgaaccgtcc cgcttccggg ttcaccctca
      241 cgcagcggcg tctctgctct ccgcaggtcg agagctgcac accgctgttc ctctcggaca
      301 gcatggcctg cgtgcgcttc tgctcccgga tggtcgtgta attgtcgagt atctgctgcc
      361 cctgccacca cagaatgctt gcgcccgatg ctgtcagcag tgtagacacc aagacgatgg
      421 tcagccacgt ctggctgacc atccgcagca tacctttcgt gcgctggctc atggcagcgg
      481 acagcttcct gtcgtggtcg aggatggcgg cgctgattct cttctcgctt tcgctcagtt
      541 ccgctctgac agacttctcg tgctcgctga atgcggcttt cagcatctcg ccggtactct
      601 gctgctgcgc tttcgatttc tgctctaagt ccttcgccag cgttaaaaga ctgttcatag
      661 atggctccct tcagtcggat gtttcgcccc ccgtccgggt cggcaatgct gatgctgctt
      721 tttgtggtgc gtaccacctc aaaacctgcg ctttccagcg cctcagtgac gtcctggcgg
      781 gttttcagct ccccagacga ggctaaggcc agtaaacccc gtgtaattgc ctgagcggct
      841 tcctgcttcg tttccggcag cgcggacggc gtgaccagcg cccgccggtt ttcaggcgcg
      901 ttcgggtcat gcagccccag cctgccgttc acgatggtct gccaggcatc aatgcgcgga
      961 cggtcggcgc ggtcgtaata cggctggaga cgcttgccgg tcagcagctc cgtgttcggg
     1021 atcaggaagt tcagctccag ccgcccctta tcctggtgct caacccacag cacgctgtac
     1081 tggtctttat cgagtccggg catcagaacc cgttcaaagc tcgccatcag cttttcgcgc
     1141 tgcccgggcg gtaaatcctt ttcggcaaag gacaggacgc cggatgtgta cttttttgcg
     1201 tagggcgagg cgtcgataag ctccctgact tcttccggct tcccctgcag aacggtggcg
     1261 ccgtcgcgct gtcggtcttt ccccagcaga taatccaccg gaccggcgcc accgccgcgc
     1321 cctcgcgggt gaaacttaac gatcatcgtc tgcacccttt tccagcacgg catgccgcag
     1381 ccgctcgagt ccggcatcta tggccatcag cgcggcgacg acctgcacgc ggtcgtgccc
     1441 gctgccacca ccggcgttaa cctgccgggc aatctggttg aggttgttcc ccatgccggc
     1501 aagctgacga agcagcgccg gcgagagcga gggaagcttg ccggcgcggg cgggtttctc
     1561 gtccaggcac gtctggcgca tccaggctgc aagctgttta ccgtcgcagc gctccagcag
     1621 acggcggtgc tcatcttcgg tcacccacat cgtgagcatt ttgctgcgtt tgtctgccaa
     1681 tccggcctcc tgataacgcc gggacgggcg ggaaatcttt tcccggtcgg cgcggcgttg
     1741 gacaagccgc aggcgcgtca gtggttttag cgggtttcgg ggcgcagccc tgaaccagtc
     1801 acgtagcgct agcggagtgt atactggctt agtatggtaa aacgaagtgg gaattcaggg
     1861 aagtgcttca tgtggcaggt aaaaaattca gctgtggggc gtaagtgcga aatgctggag
     1921 acgatgaaat gacgcctcct cgctcactcg gtcgctacgc tcctgccgtg agactgcggc
     1981 gggcgttacc tgctcaagaa tgacgagagg aatagcgctc aaatcaagaa ggaaggggaa
     2041 aaggatccgg cgttgccgtt tttccatagg ctccgccccc ctgacaagca tcacgaaatc
     2101 tgacgctcaa atcagtggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     2161 cctggtggct ccctcgtgcg ctctcctgtt cctgcctttc ggtttaccgg tgtcattccg
     2221 ctgttatggc cgcgtttatc tcattccacg cctgacactc agttccgggt aggcagttcg
     2281 ctccaagctg gactgtatgc acgaaccccc cgttcagtcc gaccgctgcg ccttatccgg
     2341 taactatcgt cttgagtcca acccgaaaag acacgactta acgccactgg cagcggccac
     2401 tggtaagcag gatgtgcgag agatgtatcg agagttcttg aagtggaggc cttagtgagg
     2461 ctacactgga aggacagtat ttggtgacat gtctcgcaca agactgttac cacggttcag
     2521 aaacttgccg gtttactgaa ccttcgaaaa accaccttgc cgggtggttt tttcgtttac
     2581 agggagagag attacgacga gagtaaaacg atcacaagga gatctgaccg tttaccagtg
     2641 atttggcttc gttttggttt ccactgaaca caaaaatagc actttttgca aaaagatgag
     2701 cagtgataga gttgtattct gtatgtgtag aagggcttgg tatgtaacct gaactctgga
     2761 ttggttacat atttgtaggc tacttgtcgg aaaccagatt tcgacaatgc gttgttaagg
     2821 tcaaagacct gtcccgaacc gaaatgctaa gggaatgcca cgaacaacac tattacgaag
     2881 aagtcggcta tttagccggc ttttttcata ttagggtatg aagttttgta cctggatggc
     2941 tcttcctatc ataagcagta atgatgctgt agtacgggtc ttcgccgttt ggttttttca
     3001 attctagtat catcatcccc gttcctgact ctacaacaat cgctttgttt tggccttttt
     3061 taggatctat gagcacttct gccccagcta ataatatgct ctccaaaaag atgacaatat
     3121 cctcagcgga agtagctcct atctcgacac gatgcttgtc ccatatatgc ctaaggccat
     3181 aggctccctc gagacttttt gtccttttgt tgtaaaaacc agaatctcct agcttgagga
     3241 tgattttccc tgcgggaaag tctgcattca tgcttggcag ttctagaaaa aattcacttc
     3301 catcacactt agccactcgt gcattttttg ttttcttatc attaagaaca gcttcaacta
     3361 tatcgcgtgg aagtaacaag ttcacaaact acacctctat agccttcact attatgttta
     3421 ggcgttaaat ccctttttga agggctgatt ctaacactga cgtaatgagc aatttccagt
     3481 aaatcttgat agaaaaaacc agttgtattt accataacat tccttgcccg caccgctccg
     3541 gacgggttca gcgtgaaagc ggcgttagcc cggtaaaacc ggcagtaaac tccccgctaa
     3601 accgcgcatt ctgcgcctga taaatttcac gtggctgcat agccatgcag ccacgtgaat
     3661 tgctcatgct ttcaatgtat atcctcgtcc aagcccctgt tttaaccggt ttatcaaaaa
     3721 accaaacaga aagccttacc actaacaata aaagaggtag gaggactgta ttttatcgca
     3781 taaaaaaaca tttacaacac gatttttatc gtatagattc cataaaaaaa tgaaattcat
     3841 tcacctatta agggtttcta tatgaaagat atgaaagctg ttgtggtttt taccgggaag
     3901 gatttgaaca taatgagaac tgaggggggg tcaggctact ggcatgccag aacagacagg
     3961 ctcaacgatg ccgattacct catagcagta agaaacagaa gggaaacgtg ggcagtaaaa
     4021 gacctggaac acggcacagc attcctgatc gccaaaatta ccgggtgttt taaaagccct
     4081 gattacgacg acaggaacgt cataaccttt gatgaatacg cagaaattca cacgccaaaa
     4141 gcatggaaaa tgctaaccga tggtcagcgc tatccggttg cttacctgag cgcacaggaa
     4201 gcttttttac gtattggggt aacaccggaa caactggaat ggaagaaatt ccatccttca
     4261 tccccatcgg ttcctaatac agtgattcct ggtctggctg aagagaaaac agaaaaactc
     4321 tcccttaacg aggcaattga aagagccaaa aaggatatta gcaacgcaac agggattgat
     4381 agctcagcca taacaataag catcaagatc tgatgaagcg tgaacaacaa gatagcagcc
     4441 tcatgttcta gaactgaacc tgtacgccag aaacagcaag cccatgcttt tccggcgcac
     4501 agattcaaag tgacggaccg ttgtacgttc gctgtcgctg agatcgttcc agctccagcg
     4561 ctttctgatg ctgcgccgcc cgttcctgct cacgatgtgc ctctatcacg ttcagccttg
     4621 tgacaaccgg cgcagctgct cgctcaaact ctgcacct
//
