LOCUS       NZ_CP032882             6548 bp    DNA     circular CON 17-JUN-2024
DEFINITION  Escherichia coli strain SCEC020022 plasmid p3_020022, complete
            sequence.
ACCESSION   NZ_CP032882
VERSION     NZ_CP032882.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN06909177
            Assembly: GCF_002165095.2
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6548)
  AUTHORS   Liu,L., Long,H., Feng,Y. and Zong,Z.
  TITLE     The complete genome of Escherichia coli strain SCEC020022
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6548)
  AUTHORS   Liu,L., Long,H., Feng,Y. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-OCT-2018) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
REFERENCE   3  (bases 1 to 6548)
  AUTHORS   Liu,L., Feng,Y. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAY-2017) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002165095.2-RS_2024_06_17
            Annotation Date                   :: 06/17/2024 07:33:24
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,250
            CDSs (total)                      :: 5,130
            Genes (coding)                    :: 4,897
            CDSs (with protein)               :: 4,897
            Genes (RNA)                       :: 120
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 233
            CDSs (without protein)            :: 233
            Pseudo Genes (ambiguous residues) :: 0 of 233
            Pseudo Genes (frameshifted)       :: 86 of 233
            Pseudo Genes (incomplete)         :: 158 of 233
            Pseudo Genes (internal stop)      :: 40 of 233
            Pseudo Genes (multiple problems)  :: 46 of 233
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP032882.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6548
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="SCEC020022"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="p3_020022"
                     /geo_loc_name="China: Sichuan"
                     /collection_date="2016-10"
     gene            complement(526..1056)
                     /locus_tag="C7V31_RS00660"
                     /old_locus_tag="C7V31_00660"
     CDS             complement(526..1056)
                     /locus_tag="C7V31_RS00660"
                     /old_locus_tag="C7V31_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000168766.1"
                     /translation="MASTEEGLLTLLLSVEHLKNTAIEQQKAVADTLAVARETLRGYRD
                     ISDSVVKQVSDEVRKGVQKTDIAGLMGERMTQDFQRLEETVAKMRANAEALNKDIATIH
                     HNVVKEYNTLNKYSLKWFIGVTVSFLVMFSLLGWFTKTLIDRNYTATEVVYQKIVDIEK
                     KLDKPATGKSRKN"
                     /name="hypothetical protein"
     gene            complement(1068..2021)
                     /locus_tag="C7V31_RS00665"
                     /old_locus_tag="C7V31_00665"
     CDS             complement(1068..2021)
                     /locus_tag="C7V31_RS00665"
                     /old_locus_tag="C7V31_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_001360358.1"
                     /translation="MATYHLNVKFGGKGQAGNHADYIERKGEYEKRKDLEYSEHGNMPG
                     WARDNPSHFWQAADQFERANGSTYREIEIALPRELTPDQRIELVQDFVRQVAGERHAYS
                     FAIHNPKASIDGGEQPHAHIMMSQRINDGMERSPEQYFRRYNAKNPERGGAKKDSGNLT
                     PTQQKEQLRTLRASWSALQNEHLARHGHRVRVDHRSLEERGIKRTPERHFGFAASSRLD
                     HAQRREILQNRAQPLEHFLEKAHSVRHQFDAQVQQKELVKTLLQDVARERGHALTARIN
                     KTGEVEFERAPTRTRSNTRGHGMGIRDNSRGYDYER"
                     /name="MobA/MobL family protein"
     gene            2201..2596
                     /locus_tag="C7V31_RS00670"
                     /old_locus_tag="C7V31_00670"
     CDS             2201..2596
                     /locus_tag="C7V31_RS00670"
                     /old_locus_tag="C7V31_00670"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001222910.1"
                     /translation="MRISNIDWLKKRIGFIRKLGEQTARQRQIIDLLDNEAGLTEQERK
                     LLHVLATAEKNELQAQENARKQANQKRMEGKTQRRERNHRLFLAAGLLIEAGLVDTKTG
                     ELRYPKDNILQKLKAIRLDLETSPDHH"
                     /name="hypothetical protein"
     gene            complement(3733..3966)
                     /locus_tag="C7V31_RS27135"
     CDS             complement(3733..3966)
                     /locus_tag="C7V31_RS27135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137389.1"
                     /translation="MTELEGHLLNALEHLQQDYMRRLNEWESAFAELQKMHEVTQRNNA
                     ILNERVVILSQQVQRLAGQVDRLSRLFIANNS"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(3973..4533)
                     /locus_tag="C7V31_RS27140"
     CDS             complement(3973..4533)
                     /locus_tag="C7V31_RS27140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_000067487.1"
                     /translation="MSNLLQMGTDFEKKLKERAASTENMLNSEFRKLEESVDKALSLNR
                     QKIRDAISEHTTSVKKQLDTLSTTVSMQFSTTEAELSRQQKKLLWRVIKGRILFPALTA
                     LSVTGGIFLGCWGLMEWQESKIAKNILTIREQENTLAKLEAKTWGVTFVNGENGKFLVL
                     PDGVKGENTWTVGDKNAVRLVRE"
                     /name="MbeB family mobilization protein"
     gene            complement(<4565..5221)
                     /locus_tag="C7V31_RS27830"
                     /pseudo=""
     CDS             complement(<4565..5221)
                     /locus_tag="C7V31_RS27830"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(5211..5534)
                     /locus_tag="C7V31_RS00690"
                     /old_locus_tag="C7V31_00690"
     CDS             complement(5211..5534)
                     /locus_tag="C7V31_RS00690"
                     /old_locus_tag="C7V31_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_001696562.1"
                     /translation="MLTIRVTDEEHARLLERCEGKRLAEWMRRVCLGEPVARTGKLPTL
                     APPLLRHLAAIGNNLNQTARKVNSGQWSSIDRVHVVAALMAIEGELRQLRQAVREQGVR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            5598..5789
                     /locus_tag="C7V31_RS00695"
                     /old_locus_tag="C7V31_00695"
     CDS             5598..5789
                     /locus_tag="C7V31_RS00695"
                     /old_locus_tag="C7V31_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_062914769.1"
                     /translation="MTKQEKTALNMARFIRNQTLTLLEKLNDLDADEQADICESLHDHA
                     DELYRSCLARFGDDGESN"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          complement(5871..5951)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="86.585"
                     /coverage="93.0"
                     /evalue="4.04e-16"
     Typing          complement(1072..2021)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.685"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(3651..5221)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.439"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tgccgtcgtc acagctcccc caaccgcaaa cggtctttgc aaacacgcaa tcatctgcaa
       61 aaacaggatt ttttcgggat tctggggagg ctgtgcggtc ggtaatgctt gaagaataca
      121 gaaggttcat gcaaactgaa tgaactggtt tccgttgtcg ccgcaacaga ctccacagga
      181 acattgagga ggtcgaaaac ctcaatgaac ccgaagaagc tcgcctaacg gcgggctttt
      241 ttcgttctac gctccctgac gaaagatcgc aagcacaata acgcgatttt tcacgctttt
      301 acgctcaaaa catcaaaaaa ccatgctttc ccccacccct acctcaaaaa acgcgtcaga
      361 aacgtttcca ggggcttgca taactattca caaaagtgat tcggaatcaa tttcgccaaa
      421 tctgagcacc gaacgggtca aaacggcaaa ctttctcatc agaaaacggt gaacgggacg
      481 cggaattttt tcgcgtcccg tgaggcgttg gacaagcgaa gcgcgtcagt ttttccggct
      541 cttccctgtt gctggtttgt ccagtttctt ttcaatatca acaattttct gatacacgac
      601 ctccgtcgcg gtgtagttcc tgtcgatcaa cgttttggta aaccagccaa gcaacgagaa
      661 catgaccaga aaactgaccg taacaccgat aaaccatttc agactgtatt tattgagcgt
      721 gttgtattct ttaacaacat tgtgatgaat tgtcgcaatg tccttgttaa gcgcctccgc
      781 gttggctctc atctttgcca cagtctcttc aagacgctga aaatcctggg tcatgcgctc
      841 acccatcagc cccgcaatgt ccgttttctg tacccctttt ctgacttcat cgctaacctg
      901 ttttacaaca ctgtcagaaa tatcccgata cccccgcagt gtttcccttg cgaccgccag
      961 cgtgtctgca accgctttct gttgttctat cgcagtgttt ttcaaatgct caacggacaa
     1021 caagagcgtg agcaacccct cttctgtact cgccatacag tcccctttta acgttcgtag
     1081 tcgtacccgc gtgagttgtc acgaatgccc atgccgtgcc ctcgcgtgtt actccgcgtc
     1141 cgcgttggcg cacgttcaaa ttcaacttca cctgttttat ttattcttgc cgtaagcgcg
     1201 tgccctcgct cccgtgcgac gtcctgtaaa agcgttttaa ctaactcttt ttgctgaact
     1261 tgtgcatcaa attgatggcg aaccgaatgc gctttctcta gaaaatgttc gagcggctgc
     1321 gctcggtttt gcaggatttc gcggcgctgc gcatgatcaa gccgtgatga ggcggcaaat
     1381 ccgaaatgcc gttctggcgt gcgtttaatt ccgcgttctt ccagtgagcg atggtcaacg
     1441 cgcaccctgt gaccatgacg cgctaaatgc tcgttctgta acgctgacca gctcgcccgt
     1501 aacgtccgca attgctcttt ttgctgagtc ggtgtcagat tgcctgaatc tttttttgcg
     1561 cctccccgtt cgggattctt ggcgttataa cgccggaaat actgttccgg cgaacgttcc
     1621 ataccatcgt taatacgctg gctcatcatg atatgcgcat gaggctgttc ccctccgtca
     1681 atgctcgctt ttggattgtg aatggcaaag ctataggcgt gccgttctcc agctacctga
     1741 cgcacaaaat cctgcaccag ttcaatgcgc tgatccggtg tcagttcacg cggcaaggcg
     1801 atttcgatct cccgataagt cgaaccattc gcacgttcaa actgatcagc cgcttgccag
     1861 aaatgggagg gattatctcg cgcccagcct ggcatattcc cgtgctctga gtattccaga
     1921 tcttttcgtt tctcgtactc gcctttgcgt tcgatgtaat cggcatggtt tccggcctgt
     1981 cccttgccgc caaattttac attgaggtga taggttgcca cggtgttagc cctccgcagg
     2041 agcgcacatg tttctgagcc aacggcgaag aaacatataa gtgcgcttcc tgctaatttt
     2101 acgaattagc acggaagata cccccctgca cccacccacc gcaagcgata tactgcacaa
     2161 ccccaacaca ccgcaaaaaa caccagggag catcccaaac atgcgcatca gcaacatcga
     2221 ctggttgaag aaacgaatcg gctttatcag gaagctcgga gaacagaccg cacgacagag
     2281 gcagatcatt gacctgctcg ataacgaagc cggactaaca gaacaggagc gcaaactact
     2341 ccacgtcctg gcaaccgcag aaaaaaacga gctacaggca caggagaacg cacgcaaaca
     2401 ggccaaccaa aaacggatgg aggggaaaac acaacgacgg gaacgcaatc accgcctgtt
     2461 tcttgcggcg gggttactga tcgaggccgg actagtggac acaaaaacag gcgaactgag
     2521 atacccaaaa gacaatatat tacagaaact taaggcaatc aggctcgatc ttgaaacaag
     2581 cccagatcat cattaattcc gctgcgtggt ggtgcgtttg cctgccggtg ttcaatgttt
     2641 tcgatttcgc gacgcgctac gcgcggatct ggcataggtg cgtacgtgct caatgtggtt
     2701 tccggttcac ttccgcttcc tcgcccactc gctcactacg ttcggtcgct ggggctgcgg
     2761 cgagcggtgg gatcactctc acatgatgca gatccttttt tttttttttg ctctggagtt
     2821 atcagttgtt gcggtgctca tgggatcgtg cgatatgctg gatggtggaa ggtcggaggg
     2881 aacggcaaca actcacccct cccagtgaag ggacgggtag tttgccgctc tcgatatgcc
     2941 ggacggagcc aaggccatcg atcacggggc gcgtcggtgg aagaacccgc gcctgcgtga
     3001 cgtatgcgtc ttcgccgcac catctcaccc gtcacgccac cacataccgg gaactgccgc
     3061 caaggtctaa ctcaaccaat ctagccgcgc tacatacggt ttttgtggca actcatcagg
     3121 tgtccgctgt cgatgaaact ctctgcgccg gtagcggaac tctcacgcgt gcggtcgccg
     3181 tcgtcacagc tcccccaacc gcaaacgatt ctcgcaaata cgcaagtatc tgcaaaaaca
     3241 ggtttttttc ggggttcctg ggagactgtg ctgttgaaaa actcttgaag aatacagaag
     3301 gctcatgcaa actgaatgaa ctggtttctg ttgtggatct caacagtttc cacaggaaca
     3361 ttgaggaggt cagagacctc aatgaacccg aaacagctcg ccttcctggc gagctgtttt
     3421 cgttctacgc ccaccgacga aagatcgcaa gtgcaataac gagatttttc acgtctttac
     3481 gctcaaaaag taaaaaaacc atgttttccc ctggctgaat ccccaaaaac gcgtgaaatg
     3541 cgttttcagg atgttgcata gctatgcaca aatacggcac acgatttgtt tcgctcgccc
     3601 cagccgtcga acgggtcaaa atggcaagat ttcgtcactt ctccggtcac atctccattg
     3661 atggcccact ccgaacccgt gccgcgcgtt cctgtacctc atgtctgtac cgggcacgct
     3721 cctgttcatc ccttagctgt tgttcgcgat aaataaccga ctcaagcgat ccacttgccc
     3781 ggctaatctc tgcacctgct gactcaatat cacgacgcgc tcgttcagta tcgcgttgtt
     3841 ccgttgcgta acctcgtgca tcttctgcaa ttccgcgaag gcgctctccc actcgttcag
     3901 ccgccgcata tagtcctgtt gcaaatgctc taaggcgttc agcaaatgcc cttccagttc
     3961 cgtcatactc gtttactccc tgaccagcct cacggcgttc ttgtcgccca ccgtccaggt
     4021 gttttcccct ttcaccccgt ccggcaacac cagaaatttc ccgttctcgc cattgacgaa
     4081 cgtcaccccc caggtttttg cctcgagttt cgccagcgtg ttttcctgct ctctgatagt
     4141 caggatattt ttcgcaatct tgctttcctg ccactccatc agcccccagc accccaggaa
     4201 aatcccgccc gtgacgctga gcgcggtcag cgccggaaac agtatccgcc ctttgatcac
     4261 ccgccagagc agttttttct gctgccggga cagttcggcc tctgtggtgc tgaactgcat
     4321 gctcacggtg gtgctcagcg tatccagttg ctttttcacg gatgttgtat gttcgctgat
     4381 agcgtctctg attttctgcc tgtttaagct caatgccttg tctacagact cttcgagctt
     4441 cctgaactcg ctgttcagca tgttctcggt agaagcggct cgctctttca gtttcttctc
     4501 gaagtctgtt cccatttgta aaagattgct catacagcgc cccctttagc ctgagattcc
     4561 gccccccctc cgggtcggca atactgatgc tgcttttggt tgtcctcacc acctcaaaac
     4621 ctgccgctgt aagcgcctct gtgacatcct gacgcgtttt tattgctccg gcatggtaaa
     4681 gagcctcaag accccgtgta atcgcttctg cggcctcctg tttcgctttt ggcagattat
     4741 ttggcgtgac aagcgtccgt ctgttctccg gtgcgttcgg gtcacgcagc ccgtaatgat
     4801 gattaaccag cgtctgccag gcatttatcc tcggcctgtc cgcacggtcg tagtacggct
     4861 ggaggcgctt accgctcagt agctccatgt tcgggatgac aaaattcagt tcgagccgcc
     4921 ccttatcctg gtgttccacc cagagaatgc tgtactggtc tttttcgaga ccgggcataa
     4981 gaacacgctc aaagctcgtc atcactcttt cgcgttcacc cggtggtagc tccttctccg
     5041 cgaacgacag aacgcctgac gtgtatttct tcgaaaatgg cgtggaatca atgagttccc
     5101 gcacttcttc cgggttaccc cggagcaccg ttgcgccttc gcggttacgc tcccgcccca
     5161 gcaggtaatc gaccgggcca cttccaccgc ctttccccct ggcatgaaat ttaactatca
     5221 tcccgtaccc cctgttccct gacagcctgc cgcaactggc gcaattcacc ctcgatggcc
     5281 atcagtgcgg ccaccacatg aacccggtca atcgaagacc actggccgct gttcaccttc
     5341 cgggctgtct ggttcaggtt gttcccgatg gcagccagat gccgcagcaa tggcggtgcc
     5401 agtgtcggca gttttcctgt gcgtgcaacc ggctccccca gacagacccg ccgcatccat
     5461 tccgccagtc gtttaccctc gcagcgttcc agtaaccggg cgtgctcctc atcggtaacc
     5521 cgtatcgtga gcatcttctc gcgtttcatc ggtatcattc cccctaacaa atcccccata
     5581 caaggagcct taagaccatg accaaacagg aaaaaacagc ccttaacatg gctcgcttta
     5641 tcagaaacca gacgctaacc ctgctggaaa aactcaacga tctggacgcg gacgaacagg
     5701 cagacatctg cgaatcactt cacgaccacg ccgacgaact ctaccgcagt tgcctcgcac
     5761 gcttcggcga tgacggtgaa agcaactgaa acatgcagct cccggagacg ttcacagctt
     5821 gcctgtgagc ggatgccggg agcagacaag cccgccaggg cgcgtcagtg cgggtgtcgg
     5881 ggcgcagccc tgacccagtt cacagagcga tagcgatgtg tatactggct ttcctcattt
     5941 tgcatcggtg cagagtggat gggaagtgca ccatgtcagg agtaaaaagc cgcacagatg
     6001 cgtaaaaaca gcgcgcccga taaacaacac acacgcggac actggcacag gcgcgtaagt
     6061 gcacaatgct tgcctctgtg actcttccgc ttcctcgccc actcgctcac tacgttcggt
     6121 cgctggggct gcggcgagcg gtgggatcac tctcgttgtg atgcagatcc gttttttttt
     6181 ttgcactgga gttatcagga gtagcggtgc acatgggatc gtgcgatatg ctggatggtg
     6241 gaaggtcgga gggaacggca acaactcacc cctcccagtg aagggacggg tagtttgccg
     6301 ctctcgatat gccggacgga gccaaggcca tcgatcacgg ggcgcgtcgg tggaagaacc
     6361 cgcgcctgcg tgacgtatgc gtcttcgccg caccatctca cccgtcacgc caccacatac
     6421 cgggaactgc cgccaaggtc taactcaacc aatctagccg cgctacatac ggtttttgtg
     6481 gcaactcatc aggtgtccgc tgtcgatgaa actctctgcg ccggtagcgg aactctcacg
     6541 cgtgcggt
//
