LOCUS       NZ_CP032881             8899 bp    DNA     circular CON 17-JUN-2024
DEFINITION  Escherichia coli strain SCEC020022 plasmid p2_020022, complete
            sequence.
ACCESSION   NZ_CP032881
VERSION     NZ_CP032881.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN06909177
            Assembly: GCF_002165095.2
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8899)
  AUTHORS   Liu,L., Long,H., Feng,Y. and Zong,Z.
  TITLE     The complete genome of Escherichia coli strain SCEC020022
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8899)
  AUTHORS   Liu,L., Long,H., Feng,Y. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-OCT-2018) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
REFERENCE   3  (bases 1 to 8899)
  AUTHORS   Liu,L., Feng,Y. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAY-2017) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002165095.2-RS_2024_06_17
            Annotation Date                   :: 06/17/2024 07:33:24
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,250
            CDSs (total)                      :: 5,130
            Genes (coding)                    :: 4,897
            CDSs (with protein)               :: 4,897
            Genes (RNA)                       :: 120
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 233
            CDSs (without protein)            :: 233
            Pseudo Genes (ambiguous residues) :: 0 of 233
            Pseudo Genes (frameshifted)       :: 86 of 233
            Pseudo Genes (incomplete)         :: 158 of 233
            Pseudo Genes (internal stop)      :: 40 of 233
            Pseudo Genes (multiple problems)  :: 46 of 233
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP032881.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8899
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="SCEC020022"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="p2_020022"
                     /geo_loc_name="China: Sichuan"
                     /collection_date="2016-10"
     gene            complement(1373..1606)
                     /locus_tag="C7V31_RS27120"
     CDS             complement(1373..1606)
                     /locus_tag="C7V31_RS27120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_162984795.1"
                     /translation="MTELEIHLLNALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSEQTEHLSQLYSESRR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(1613..2131)
                     /locus_tag="C7V31_RS27125"
     CDS             complement(1613..2131)
                     /locus_tag="C7V31_RS27125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_162984796.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKVLNNEFRRLGESVSEAVTSNE
                     TKIRDAIALFTASTEESLEKHREGVKEAMRQHRKDVLKLAGNTGMMLLGIVFLLFTASG
                     GTLWYLGGRIQANLEDIRKQEETLQKLNAKTWGVEFVQDGNRKFLVLPCGKSAEVIPFQ
                     GKEWVHLKE"
                     /name="MbeB family mobilization protein"
     gene            complement(<2169..2867)
                     /locus_tag="C7V31_RS28220"
                     /pseudo=""
     CDS             complement(<2169..2867)
                     /locus_tag="C7V31_RS28220"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(2809..3132)
                     /locus_tag="C7V31_RS00605"
                     /old_locus_tag="C7V31_00605"
     CDS             complement(2809..3132)
                     /locus_tag="C7V31_RS00605"
                     /old_locus_tag="C7V31_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000955999.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            3197..3388
                     /locus_tag="C7V31_RS00610"
                     /old_locus_tag="C7V31_00610"
     CDS             3197..3388
                     /locus_tag="C7V31_RS00610"
                     /old_locus_tag="C7V31_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165974.1"
                     /translation="MTKQEKTALNMARFIRGQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            4058..4216
                     /locus_tag="C7V31_RS28225"
     CDS             4058..4216
                     /locus_tag="C7V31_RS28225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001354639.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDKAPLAAAVGNLVSRAMMA
                     QMVQQSS"
                     /name="hypothetical protein"
     gene            4598..4939
                     /locus_tag="C7V31_RS00625"
                     /old_locus_tag="C7V31_00625"
     CDS             4598..4939
                     /locus_tag="C7V31_RS00625"
                     /old_locus_tag="C7V31_00625"
                     /inference="COORDINATES: protein motif:HMM:NF015489.4"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E1 family microcin immunity protein"
                     /protein_id="WP_000058758.1"
                     /translation="MSLRYYIKNILFGLYCTLIYIYLITKNNEGYYFLASDKMLYAIVI
                     STILCPYSKYAIEHIFFKFIKKDFFRKRKNLNNAPVAKLNLFMLYNLLCLVLAIPFGLL
                     GLFISIKNN"
                     /name="colicin E1 family microcin immunity protein"
     gene            complement(4936..6490)
                     /locus_tag="C7V31_RS27825"
                     /pseudo=""
     CDS             complement(4936..6490)
                     /locus_tag="C7V31_RS27825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447006.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0140911 - pore-forming activity [Evidence
                     IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA];
                     GO:0050829 - defense response to Gram-negative bacterium
                     [Evidence IEA]; GO:0031640 - killing of cells of another
                     organism [Evidence IEA]"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like pore-forming protein"
                     /name="colicin-like pore-forming protein"
     gene            6768..7187
                     /locus_tag="C7V31_RS00640"
                     /old_locus_tag="C7V31_00640"
     CDS             6768..7187
                     /locus_tag="C7V31_RS00640"
                     /old_locus_tag="C7V31_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000312320.1"
                     /translation="MAWHTLQNALKMTGRSRSQFYRDMRAGLVSYRTGQDGRREFETSE
                     LIRAYGELKQDETPERHSAGQAENPHDQQTERILRELNELRQCLTLMLEDKQAQATERR
                     RQEAEREQLHKEIEQLRQALEQEKKRGFWARLFSR"
                     /name="hypothetical protein"
     gene            7271..7690
                     /locus_tag="C7V31_RS00645"
                     /old_locus_tag="C7V31_00645"
     CDS             7271..7690
                     /locus_tag="C7V31_RS00645"
                     /old_locus_tag="C7V31_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_001254598.1"
                     /translation="MRRSGGTLYLIALLTAATVLTACTPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLRGTTQ
                     FAGTDYRSKDLTPKKSRLLAETISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            8216..>8392
                     /locus_tag="C7V31_RS00650"
                     /old_locus_tag="C7V31_00650"
                     /pseudo=""
     CDS             8216..>8392
                     /locus_tag="C7V31_RS00650"
                     /old_locus_tag="C7V31_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611469.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0004803 - transposase activity [Evidence IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /name="transposase"
     Typing          complement(3474..3561)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="97.753"
                     /coverage="100.0"
                     /evalue="1.48e-35"
     Typing          3584..4130
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.011"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(1270..2819)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.42"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tcgaaaacac ggattttagt atgcacatga ctgtactcct gtggagtaca aggaaatcat
       61 aatcagcgac caaaccattt atcaacagaa aaccaacata ttgatgttat ttttgtgaca
      121 aatgttacaa attcgaaacc actgtatttt tactgcaaca aaatctggtt gatctacaag
      181 ttacactggg gtaaaaagtt gtccggaatg tttctgcgta gtaaagcctc tagccggatt
      241 gcctttgcaa aaacgcagga acgttccccc cttcatcagt actcctgacg ctgtaaggag
      301 ctgactgatg cagacagggc tgatgatatc taataaaagc tatctcattt ttgcgcgtac
      361 ataaaataaa tcaggcactt ccctacactc cccctccgga attgaggggg ttttctttac
      421 aatgtaaata aattgcttgc gtaaagttca aaaaaactgc aaatctttac tgtagtgtcg
      481 agcttcatgt agctcaccag ggcatggact aaaaagaagt aaacccatct tttacccccc
      541 cttatacggg gggttcttta tagcacaaag aacacttata atttatcatc tcacagctat
      601 aaatataaaa gaataatatt ctaaactaca gttatttatg cagaacataa catcaaactc
      661 acaataaata taaaacaatt attttataac tgttcactta ttatattcag acatatcaga
      721 aacacgactc aattcttttt tatttaacaa aatccataaa aaacttatgg ttgaaatcac
      781 atttaaactg tgaaggatat catccggaga aatttttcaa tccgacactc catagaatgg
      841 acctttaaga ctttatcctc atcatgtatg aggatttttt ttctggcaaa cagtatcttt
      901 cagtaatcct tgctcagttc cgattctgac gtatccccgt gtggatctga aaaggagata
      961 attacgctct gtggccaaca ggaataactc aagcagctcc tctctgatac catcagaaga
     1021 agcattaaac gcattctggc gtcgattaca gaagaagcgg aacgcgttcc aggctcctgt
     1081 tgctatacac actacccaca ctaaccccat ggtttttgag cgccagaaac gcgtccgtat
     1141 gcgttttaac gcgttcctgt gttttcagca aggcctgact gaagctagtt cctagtgccc
     1201 tgaaccaacc attatccaaa aaaatgcacc ggaaacgcgc tgacaacact ttagcctgtc
     1261 tttggttacc atcccagcga cgggccatcc agtgtccgtt cctgtatttc cagatggcgc
     1321 tcacgctcta ggaatagctc atgttcacgt tcctgtatca gtagttcgtc ccttatctcc
     1381 tgctctcgct gtataactgg ctcaagtgtt ctgtctgctc gctcaagtgc tgcacctgct
     1441 gactcaactg catgacccgc tcgttcagca tcgcgttgtc ccgttgcgta agcgaaaaca
     1501 tcttctgcaa ttccacgaag gcgctctccc attcgctcag ccgctgcata tagtcctgtt
     1561 gcagctgctc taaggcgttc agcaaatgta tttccagctc tgtcactctg tgtcactcct
     1621 tcagatgtac ccactctttc ccctgaaagg gaatcacctc cgctgatttc ccgcacggaa
     1681 ggacaaggaa tttcctgttc ccgtcctgca caaactccac gccccatgtc ttcgcgttca
     1741 gtttctgcaa tgtctcttcc tgcttcctga tatcttccag gttcgcctgt atcctcccgc
     1801 caagatacca gagcgtcccg ccactcgcgg taaacaggag aaagactatc cccagtaaca
     1861 tcatgcccgt attccctgcc agctttaaca cgtccttcct gtgctgcctc atcgcctctt
     1921 tcaccccttc ccggtgtttc tccagcgatt cctctgtcga ggctgtgaac agggctatag
     1981 cgtctctgat tttcgtctcg tttgatgtca cagcctcgct tacagattcg ccgagcctcc
     2041 tgaactcgtt gttcagcact ttctctgtag attcggctct ctctttcagc tttttctcga
     2101 actccgcgcc cgtctgtaaa agattgctca taaaacgctc ctttcagcct gatattcttc
     2161 ccgccgttcg ggtctgcaat gctgatgctg cttcgcgtca ccctgaccac ttcaagcccc
     2221 gcctctgtga gcgcctgaat cacatcctga cggcctttta tctcccctac atgataaaga
     2281 gcgtctatcc cgcgtgtgac gctctcagca agcgcctgtt tcgtttccgg caggttatca
     2341 gagagagtca gtatccggcg gttctccggg gcgttggggt catgcagccc gtaatggtga
     2401 ttcaccagcg tctgccaggc atcaattctc ggcctgtctg cccggtcgta gtacggctgg
     2461 agacgttttc cggtctgtag ctccatgttc ggaatgacaa aattcagctc aagccgtccc
     2521 ttgtcctggt gctccaccca caggatgctg tactgattct tttcgagacc tggcatcagt
     2581 acgcgctcaa agctcgccat cactttttca cgtcctcccg gcggcaactc cttctccgcg
     2641 aacgacagaa cgcctgacgt gtatttcttc gcaaatggcg tggcatcgat gagttcccgg
     2701 acttcttccg gattaccctg aagcaccgtt gcgccttcgc ggttacgctc cctccccagc
     2761 aggtaatcaa ccggaccact gccaccacct tttcccctgg catgaaattt aactatcatc
     2821 ccgcgccccc tgttccctga cggccagacg cagccggcgc agctcatccc cgatggccat
     2881 cagtgcggcc accacctgaa cccgatcacc ggaagaccac tgcccgctgt tcaccttcct
     2941 cgcggtctga ttcaggttat tcccgatggc ggccagctga cgcagtaacg gcggtgccag
     3001 tgtcggcagc ttcccggaac gggcaaccgg ctcccccagg cagacccgcc gcatccatac
     3061 cgccagttgt ttaccctcac agcgttccag taaccgggca tgttcatcat cagtaacccg
     3121 tatcgtgagc atcctctcgc gtttcatcgg tatcattacc cccatgaaca gaaatccccc
     3181 ttacacggag gcatcagtga ctaaacagga aaaaaccgcc cttaacatgg cccgcttcat
     3241 cagaggccag acattaacgc tgctggagaa actcaacgaa ctggacgcgg atgaacaggc
     3301 agatatctgt gaatcgcttc acgaccacgc cgatgagctt taccgcagct gcctcgcacg
     3361 tttcggggat gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg
     3421 tctgtgagcg gatgccggga gccgacaagc ccgtcagggc gcgtcagcag gttttagcgg
     3481 gtgtcggggc gcagccctga cccagtcacg tagcgatagc ggagtgtata ctggcttagt
     3541 catgcggcat cggtgcggat tgtatgaaaa gtgcaccatg tacggtgtga aatgccgcac
     3601 agatgcgtaa ggagaacatg cagatgccga tgctcttccg cttcctcgct cactgactcg
     3661 ctgcgctcgg tcgttcggct gtggcgagcg gtgtctgctc actcaaaagc ggtgatactg
     3721 ttatccacac aatcagggga taacgccgga aagaacatgt gagcaaaaaa cgaagacccc
     3781 agaaaaggcc gcgcaggagg cgctttttcc ataggctccg cccccctgac gagcatcaca
     3841 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg acttaaagat accaggcgtt
     3901 tccccccgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     3961 ctccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gttggtatct
     4021 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     4081 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgacaa
     4141 agcgccactg gcagcagccg ttggtaactt ggttagcaga gcgatgatgg cgcaaatggt
     4201 gcaacagagt tcttgaagta gtggcccgac tacggctaca ctggaaggac agtatttggt
     4261 atctgcgctc tgctgaagcc agttacctcg gttaagcagt tccccaactg acttaacctt
     4321 cgatcaaacc gcctccccag gcggtttttt cgtttacagg cagcagatta cgcgcagaaa
     4381 aaaaggatct caagaagatc ctttgatctt ttctactgaa cagagatccc cgtcagttta
     4441 gaagaagatg atggagccac agtccctccc tgaacatcac gtatatagtt agcctgacat
     4501 ccaacaagga ggtttatagc taatattccc acaaaaaatc ttttcctcat aactcgatcc
     4561 ttataaaatg aaaagaatat ataacgaggt ttaatttatg agtttaagat actacataaa
     4621 aaacattttg tttggcctat attgcacact tatatatata taccttataa caaaaaacaa
     4681 cgaagggtat tatttcctag cgtcagataa gatgctatac gcaatcgtga taagcactat
     4741 tctatgccca tattcaaaat atgctattga acacatattt tttaagttca taaagaaaga
     4801 ttttttcaga aaaagaaaaa atctaaataa tgcccccgtg gcaaaattaa acctatttat
     4861 gctatacaat ctactttgtt tggtcctagc aatcccattt ggattgctag gactgtttat
     4921 atcaataaag aataattaaa tacccaacac atcatttata gtattaagtt tattcttgtc
     4981 aataaaggca catagaatgc ctgtaacaat agcaatcccc cagataccta atgtagttcc
     5041 agcaagcaca ctaaaacaat ataactaact ccagcatcta cagttttctt ctctaatgta
     5101 agaaatagtg gcttccagtc acctgtatcc ttaattttta ggatatcaga taccacatca
     5161 tatccaaaag aaacatgtcc cgtaatcttc aagtacttag caaactgatc taaatgctta
     5221 gcccagtctt catacttaac cgattccagt gcattgaaaa tcgcatcacg gtctgctttg
     5281 ctgaatttct tatttaaaac atccttgtat ttttcaaaag cagctagagc ttcattcaca
     5341 ttgctgattt tcttaccttt agacttatca gcaagttcct gtgccatttt tgaatatttt
     5401 tcaccatatt tttcagatag cgtttgataa aagctaactg ttgcatccac agcatcctta
     5461 atctgcgagt tcaggagatt agtctgcgct gttttcaaat tttcttcaga ttcatgaaca
     5521 cgtaaaacag ctgtattacg attattatta gcctgagaca tagccccctg aattccattt
     5581 atctcagcat taagttggtt aatacgcgtt tctgatgctg ttacctgttt ttgttttcct
     5641 ccataatctt accggctccc gtccgtcgac tggtggcata gaaaaaagga cgactctgaa
     5701 gcggatcatt agctcttggt gacaattttt tgaccagttc atcaagttcc ttatatttag
     5761 cagatgcctg agccagctca tttcgttttc cggacagcgt attcatttct gcatcacggg
     5821 catgaatact ggagcttaaa cgggtattga gagtcttaat ctcttcatcc actttcgcca
     5881 gctcagattg tgcagcagca agattttttt gtgccacctc cactgcccgg gcctcttcat
     5941 tcaatgctgc cagccgtttc tcttcagctt cagccagttt caactggcgt tctgtttcag
     6001 cctgctcctt ctctatctct ttgcgtcgtt gttctgcctc ctgaaaagcc ttttccgcag
     6061 cttccgcttc tttacgggct ttttcttctg cttttgcaag gcgcaaccgc tctgcttctg
     6121 cctgcatcgc tgcattattg gcatgagcca ggtcaataac ctccggatga gtggaattat
     6181 gacgaagcgc ctcattcaca atatccttca gatgttgagt cagcgcatcc cggtttgctt
     6241 ttgctttagc ctgtgcttct gcggcagctt ttgcccgggc agcctgttct gcctgcgttt
     6301 tcttcaattg agcagtagac catttagctg tggcatgaat ggctgcagaa ctttcacttt
     6361 tgctacctcc agttccacca ccgccgccag agccactccc gtctggatta ccattcaaaa
     6421 gagtaatgat tacctgcccc ttatcatcat aaggaacacc atctttatag tacgctacag
     6481 ctgtttccat tataaaatcc tctttgacat taaaaacaaa tagttaaaag caatatactg
     6541 tatatataac tactggtttt atatacagct taaaattacg ccgctgcatt ttccctgtca
     6601 accctgtgga ttttcatttt tgtgaaaacg atcaaaaaaa cagccaccca caccacgtgc
     6661 tattcccacc cgatgccaca aaaaccagca caaacattac cgttctccgg ccccagtatg
     6721 ttttactgaa accatgatat gagacattaa tgggacactg tcacttcatg gcatggcaca
     6781 cactccagaa cgcactgaaa atgacaggca gatcgcgttc acagttttac cgtgatatgc
     6841 gcgcaggcct tgtcagttat cgtacaggcc aggacggacg gcgggagttt gaaaccagtg
     6901 aactgatccg ggcatacggc gaattaaagc aggatgagac accagaaagg cacagtgcgg
     6961 gacaggcaga aaatccacat gatcagcaga cagaacgcat tctccgggag ctgaatgagc
     7021 tgagacaatg cctgacactg atgcttgagg acaaacaggc acaggctacg gaacgccggc
     7081 gccaggaagc cgaacgggaa cagctacata aagagataga gcagctcagg caggcactgg
     7141 agcaggaaaa aaaacgggga ttctgggcca gactgttcag ccggtgagca ccgactgcaa
     7201 gtgatgataa gatagtcagc ggttaaatat aagtcactga gtacaaagat ttaaaggtaa
     7261 ggagacactc atgagacgtt ccggcggcac actgtatctt atcgccctgc tgacagcggc
     7321 aacggtcctg acagcctgca cgccaaaggg cagtgtggaa caacataccc ggcattacgt
     7381 ttatgcatca gacgatggtt ttgatcctaa cttttccacc cagaaggccg acacaacacg
     7441 aatgatggtg ccttttttcc ggcagttctg ggatatggga gcaaaggata aagcgacagg
     7501 aaaatcacgg agtgatgtgc agcaacgcat tcagcagttt cacagccagg aatttttaaa
     7561 ctcactccgg ggcacaactc aatttgcggg tactgattac cgcagcaaag accttacccc
     7621 gaaaaaatcc aggctgctgg ctgagacgat ttctgcggtt tatctcgatg gctacgaggg
     7681 aagacagtaa gtggatttac cataatccct taattgtacg caccgctgaa atgcattcag
     7741 cgcaatcacg gcagcagaca ggtaaaaatc gcaacaaccc ccccgaaaaa ctaccgcgat
     7801 cgcgcctgat aaattttaac cttatgcata cctatgcatc caggtgaatc acaagcgaat
     7861 ttccttactg agacgactga aacggttttt tttcacaatt ttcagctgtt ttcgattcca
     7921 caaaatagaa gttacaggat agggattaat ccccttcgcc gccaagggga gcaggatgag
     7981 tcaggggaat gtctattcac ctgcgctgta caggctgcca ggtaaaagta ttcgctgcgg
     8041 cacgatactc ttttgccctc atagcctttg cgcttcttac ttttcgttct tccagaatca
     8101 ttcttaagac attttcccgc acttttctct aacttcccgt ggcaatttat ctctgccgga
     8161 ctgaaccgcc ccggaaatcc tggagactaa acttcctgag aaagatgtaa acaggatgac
     8221 taaaaatact cgtttttccc ccgaagtccg tcaacgggca gttcgtatgg ttctggaaag
     8281 ccagggcgaa tatgactcac aatgggcggc aatttgttcc attgctccaa agattggctg
     8341 tacgccagag accctgcgtg tctgggttcg ccagcatgag cgggataccg ggtattacct
     8401 ttcgaagaaa tttttgacat cctctacgcc ctgaaggacc gggttttacg gcgtaccaga
     8461 taaaaaaacc cctctactga agaggggtag tgtaaggaac ttgctgattt tcttgatatt
     8521 attaaccatc aataactgag catacaaaaa ataacaaaac tctgacacag atacaaccat
     8581 ccctctttgt ataagaccga tgtgcatctc aaaaaagaag cgtgtttctg gaacacagaa
     8641 caacaatgca cattgtagcc aacaaattca caccagaaac acgctctgtt ttttaattta
     8701 cacaagaata tatgtctgga aaatcaggaa gaagaaatta atgggcattc tgtatacgaa
     8761 gggagagcgc gtttccggtg ccccatatta gatattatcc ctgcaacaaa caccagagac
     8821 gcgctcataa ttcttctagc gtaaatgatc ctttcaggca agaaaaaagc cgtgattctg
     8881 tatcacggcc ttaatttta
//
