LOCUS       NZ_CP032845             4048 bp    DNA     linear   CON 14-APR-2024
DEFINITION  Enterobacter hormaechei strain C15117 plasmid unnamed3.
ACCESSION   NZ_CP032845
VERSION     NZ_CP032845.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN10174734
            Assembly: GCF_003660125.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 4048)
  AUTHORS   Chowdhury,P.R., Fourment,M., DeMaerre,M.Z., Monahan,L.G.,
            Merlino,J., Gottlieb,T., Darling,A.E. and Djordjevic,S.
  TITLE     The complete genome of Enterobacter hormaechei C15117
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4048)
  AUTHORS   Chowdhury,P.R., Fourment,M., DeMaerre,M.Z., Monahan,L.G.,
            Merlino,J., Gottlieb,T., Darling,A.E. and Djordjevic,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-OCT-2018) Ithree Institute, University of Technology
            Sydney, Building 4 Harris St, Sydney, NSW 2007, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: MAR-2018
            Assembly Method :: Unicycler v. 0.4.3; SPAdes v. 3.11.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 7.8x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_003660125.1-RS_2024_04_14
            Annotation Date                   :: 04/14/2024 18:49:22
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,934
            CDSs (total)                      :: 4,819
            Genes (coding)                    :: 4,722
            CDSs (with protein)               :: 4,722
            Genes (RNA)                       :: 115
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 45 of 97
            Pseudo Genes (incomplete)         :: 61 of 97
            Pseudo Genes (internal stop)      :: 18 of 97
            Pseudo Genes (multiple problems)  :: 23 of 97
            Pseudo Genes (short protein)      :: 1 of 97
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP032845.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..4048
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="C15117"
                     /isolation_source="Burns unit surveillance"
                     /db_xref="taxon:158836"
                     /plasmid="unnamed3"
                     /geo_loc_name="Australia: Sydney"
                     /lat_lon="33.8688 S 151.2093 E"
                     /collection_date="2007"
                     /collected_by="Concor Hospital Microbiology Laboratory"
                     /note="1"
     gene            <1..271
                     /locus_tag="D8M16_RS25120"
     CDS             <1..271
                     /locus_tag="D8M16_RS25120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_121479479.1"
                     /translation="DGRQLAAWMRQTCLDEKPARSGKLPSISPALLRQLAGMGNNLNQI
                     ARRVNAGGGTGHDRVQIVAALMAIDAGLERLRHAVLEKGTDDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            480..>914
                     /locus_tag="D8M16_RS25760"
                     /pseudo=""
     CDS             480..>914
                     /locus_tag="D8M16_RS25760"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            949..1434
                     /locus_tag="D8M16_RS25325"
     CDS             949..1434
                     /locus_tag="D8M16_RS25325"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_045326458.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVKAELNESA
                     KRISAAILDHDRKLSSAMSQRTKGMVRMVSQTWLTIVLVSALLIASSAGILWWQGQQIL
                     DNYTTIREQKSTQAMLSERNSGVQLSTCGEQRRRCVKVNPEAGRFGEDSSWMILAGK"
                     /name="MbeB family mobilization protein"
     gene            1438..1653
                     /locus_tag="D8M16_RS25330"
     CDS             1438..1653
                     /locus_tag="D8M16_RS25330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_015589619.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFAEWRTMSGLMQRENA
                     ALSERVTGLSRQVQSLSEQLRRLSSD"
                     /name="MbeD family mobilization/exclusion protein"
     gene            1999..3555
                     /locus_tag="D8M16_RS25130"
     CDS             1999..3555
                     /locus_tag="D8M16_RS25130"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013199864.1"
                     /GO_function="GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]; GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="WP_239507231.1"
                     /translation="MRIKKPHLRPIVITLSVGGILLTSIFLIAVIMVFQRENIEKDLLD
                     ANSSYAMKMSDVMSGYIEMAQGELAYGAKKIKSTNDIDSLKDEADRLRLQSGMFNSVFV
                     VSNDAVVLATSPESLDLVGTRLNSSASKLAIEEKKPFISSPFKSVTGNLIILLSHPIYD
                     KNGNYIGHVGGSIYLKKHSLFSDVLSRHFFKSDTEISIVSDDGTVIFNNDNSAVGRPMV
                     MSDDLKSKLTESDRGEGEFFFSGNRYLLGYAHMKNTAWNVLVYSHADSATTILIAYAKS
                     VLVLLIVIIITFSLLSYFIASRISLPLEKLAISTNAKDIGASLVYIKAINAWYAEADRL
                     KKALFTHVKVMMERVDSLNEVALKDPLTGVNNRLGFSTKIQNNKYGAGASVIAIDVDFF
                     KKINDSFGHGVGDEVLVSLAQIIKSCCRSEDIICRFGGEEFVVFLPNTSVVTAECVAER
                     IRSVIERTVFPNNLRVTVSAGVATQDDPLGGIDFLLKNADDALYQAKREGRNKVIVYSA
                     I"
                     /name="sensor domain-containing diguanylate cyclase"
     Typing          complement(3859..4047)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.542"
                     /coverage="81.0"
                     /evalue="2.9e-57"
     Typing          3..265
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.045"
                     /coverage="82.0"
                     /evalue="2.07e-47"
     Typing          262..1757
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="90.982"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 cgacggcagg cagctggcag cgtggatgcg gcagacctgc ctggacgaaa agccggcacg
       61 ttccggaaag cttccctcaa tctcgccggc gctgctgcgc cagcttgccg gcatgggcaa
      121 taacctgaac cagatagccc gccgtgttaa cgccggcggc ggtaccggcc atgacagggt
      181 gcagattgtt gcggcgctga tggccatcga tgccggactt gagcggctgc ggcacgccgt
      241 tcttgagaaa ggaacggacg atgatcgtta aatttcatcc acgcggtcgc ggcggtggcg
      301 ccgggccggt ggactatctg ctgggtaaag accggcagcg tgaaggcgcc accgtcctgc
      361 aggggaagcc ggaagaagtc cgggagctga ttgacgcgtc gccttacgtt aagaaataca
      421 cctccggggt actgtcgttt gcggaagcgg atttaccctc cggccagcgg gaaaagctga
      481 tggcaagctt tgagcgggtt ctgatgcccg gacttgataa agaccagtac agcatcctgt
      541 gggtggagca tcgtgacaag gggcggcttg agctgaattt tctgatcccg aacacggagc
      601 tgctgaccgg cagacggctc cagccgtatt acgatcgcgc cgaccgtcca cgcatcgatg
      661 cctggcagac catcgtgaac ggcagactgg ggctgcacga cccgaacgca ccggagaacc
      721 ggcgggcact ggtcacaccg tctgcactgc ctaaagcgaa gcaggaggct gcagaagcga
      781 ttacacgcgg tctgcttgct cttgcctcat ccggggagct gaaaacccgt caggatgtca
      841 ctgaggcgct ggaaagcgca ggttttgagg tggtccgcac cacaaaaagc agtatcagca
      901 ttgccgaccc ggacgggggg cgaaacatcc gactgaaggg agccatttat gaacagtctt
      961 ttaacgctgg cgaaggactt agagcagaaa tcgaaagcgc agcagcagag taccggcgag
     1021 atgctgaaag ccgcattcag cgagcacgag aaatctgtaa aagcggaact gaacgaaagc
     1081 gcgaagagaa tcagcgccgc catcctcgac cacgaccgga aactgtcctc agccatgagc
     1141 cagcgcacga aaggcatggt gcgcatggtc agccagacgt ggctgaccat cgtcctggtc
     1201 tccgcgctgc tgatcgcctc gagcgcgggc attctgtggt ggcaggggca gcagatactc
     1261 gacaactaca cgaccatccg ggagcagaag agcacgcagg ccatgctgtc agagaggaac
     1321 agcggcgtgc agctctcgac ctgcggcgag cagagacgcc gctgcgtgaa ggtgaacccg
     1381 gaagcgggac ggttcggaga ggattcgagc tggatgatac tggcggggaa atagcacatg
     1441 acggagctgg aaaaacagtt gctgagcgca ttagagcagt tacagcagga ctactcgaaa
     1501 aggctggacg agtgggagag cgccttcgcg gaatggcgga cgatgtctgg tcttatgcaa
     1561 cgggagaacg cagcgctgag cgagcgcgtc acgggcttga gcaggcaggt gcagagtttg
     1621 agcgagcagc tgcgccggtt gtcgagcgac tgaatgccgt cgagctgcac cgggagcagg
     1681 agcgcgtggt gcagcatcag aaagcgctgg agctggagcg atctcagcga cagcggacgt
     1741 ataacagccc ttcactgtga taacctgata aaacggggct ttcggtggac atctgcgctg
     1801 aaagccggag ccattcaccc gactgcatag ccatgcagcc gggtgaaatt tatctggcgc
     1861 agaatgcgcg gtttagcgga gagtttgctg ccggttttac cgggctgacg ccgcatttgt
     1921 tccgaacccg tccggggcgg tgctgataag ggatgttatg gtaaatgcgg cattttttat
     1981 tatatggagt gtatttttat gagaattaaa aagcctcacc ttcgtccgat agtcataacc
     2041 ttgtccgttg gtgggatttt gttaacttct atttttctga ttgccgttat tatggttttt
     2101 cagcgcgaaa atatagaaaa agatttgctt gatgctaatt catcgtacgc tatgaaaatg
     2161 tcagatgtaa tgagtggtta tattgaaatg gctcaaggtg aattagcata tggagcaaaa
     2221 aaaataaaat caactaatga tattgactca cttaaagatg aggcggatag gttaagattg
     2281 cagtctggta tgtttaattc agtttttgtt gttagtaatg atgctgttgt tctggcgact
     2341 tcgccagaat ctcttgattt agttggtacc cgcttgaatt caagtgccag caaattggcg
     2401 attgaggaaa agaaaccatt catatcttca ccgttcaaat cagttactgg gaatttgatt
     2461 attttgttgt cacatcctat ttatgataag aatggcaatt acatagggca tgtcggtggg
     2521 tctatatatc taaaaaaaca tagtttattt agcgatgttt tgagcaggca ttttttcaag
     2581 agcgacactg aaattagcat tgttagtgat gatggaactg tgatctttaa taacgataac
     2641 agtgctgttg gtcggcccat ggttatgtct gatgatttga aatcaaagtt aacggagtct
     2701 gatagaggtg agggggaatt ttttttttcg gggaatagat atcttctggg ttatgctcat
     2761 atgaaaaata ctgcttggaa tgtgttggtt tatagccatg ctgacagcgc gactactatt
     2821 cttattgctt atgctaaaag tgtacttgtt ctgctaattg taataattat tacattttca
     2881 cttctttcat atttcattgc ctcccgtatt tcacttcctt tagaaaagct tgctatatct
     2941 accaatgcta aagatatagg ggcttcgtta gtttatatta aagctatcaa tgcttggtat
     3001 gccgaagctg atcgacttaa aaaggcactt tttacgcatg ttaaggtaat gatggaaagg
     3061 gttgattctt taaatgaggt ggcgcttaaa gatcccctta ccggcgtgaa caatagattg
     3121 gggttttcaa ctaaaataca gaataacaag tatggggcag gagcttcagt tattgctatt
     3181 gatgttgatt tttttaaaaa aataaatgat agtttcgggc atggggtcgg agatgaggtg
     3241 cttgtttctt tggctcagat tattaagtct tgttgccgta gtgaagatat tatttgtcgg
     3301 tttggtggag aagagtttgt tgtttttctg ccgaatacat ctgtggtaac agcggagtgt
     3361 gttgcggagc gtattaggag tgttattgaa cgtactgttt tccctaataa cttgcgtgtc
     3421 accgtatctg ctggtgtggc aacacaagat gatcctcttg gtgggatcga ttttttgctg
     3481 aaaaatgctg acgatgctct ttatcaagct aagagagagg ggcgtaataa agtgattgta
     3541 tatagcgcta tttgagtttt tttgatgacg ctttacgcgc tagtgtgccg tttttttttg
     3601 tggggagggg ggttcaagaa gcggatcctc ttgttatccc ttcgatgtcg tcgtaatctc
     3661 atgccctgca aacaaaaaaa ccaccgctac caacggtggt tcgtttgccg gaatcaagag
     3721 ctaccaactc tttcttccga aggtaactgg ctttcacgga gcgcaggtac caaaactgtc
     3781 cttccagtgt agccgcagtc aggccaccac ttcaagaact ctgtagaaca ctctactact
     3841 ccgctccgtg aatcctctta ctaatggcta ccgccactgg cgatttttcg tgtctgttcc
     3901 gggttggact caagatgata gttaccggga caacgtggta gtcgggctga acggggggtt
     3961 cgtgcataca gtccagcttg gagcgaactg ccttcccgga accgagtgtc aggcgtggaa
     4021 tgagataaac gcggccataa cagcggat
//
