LOCUS       NZ_CP032843             6237 bp    DNA     circular CON 14-APR-2024
DEFINITION  Enterobacter hormaechei strain C15117 plasmid unnamed1, complete
            sequence.
ACCESSION   NZ_CP032843
VERSION     NZ_CP032843.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN10174734
            Assembly: GCF_003660125.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 6237)
  AUTHORS   Chowdhury,P.R., Fourment,M., DeMaerre,M.Z., Monahan,L.G.,
            Merlino,J., Gottlieb,T., Darling,A.E. and Djordjevic,S.
  TITLE     The complete genome of Enterobacter hormaechei C15117
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6237)
  AUTHORS   Chowdhury,P.R., Fourment,M., DeMaerre,M.Z., Monahan,L.G.,
            Merlino,J., Gottlieb,T., Darling,A.E. and Djordjevic,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-OCT-2018) Ithree Institute, University of Technology
            Sydney, Building 4 Harris St, Sydney, NSW 2007, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: MAR-2018
            Assembly Method :: Unicycler v. 0.4.3; SPAdes v. 3.11.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 7.8x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_003660125.1-RS_2024_04_14
            Annotation Date                   :: 04/14/2024 18:49:22
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,934
            CDSs (total)                      :: 4,819
            Genes (coding)                    :: 4,722
            CDSs (with protein)               :: 4,722
            Genes (RNA)                       :: 115
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 97
            CDSs (without protein)            :: 97
            Pseudo Genes (ambiguous residues) :: 0 of 97
            Pseudo Genes (frameshifted)       :: 45 of 97
            Pseudo Genes (incomplete)         :: 61 of 97
            Pseudo Genes (internal stop)      :: 18 of 97
            Pseudo Genes (multiple problems)  :: 23 of 97
            Pseudo Genes (short protein)      :: 1 of 97
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP032843.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6237
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="C15117"
                     /isolation_source="Burns unit surveillance"
                     /db_xref="taxon:158836"
                     /plasmid="unnamed1"
                     /geo_loc_name="Australia: Sydney"
                     /lat_lon="33.8688 S 151.2093 E"
                     /collection_date="2007"
                     /collected_by="Concor Hospital Microbiology Laboratory"
                     /note="1"
     gene            615..1151
                     /locus_tag="D8M16_RS25055"
     CDS             615..1151
                     /locus_tag="D8M16_RS25055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005200081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_072159713.1"
                     /translation="MLDTDCITVLTARGITEILNSGGSQAWRLDATHVAKLPYLVCVQN
                     SKKDWGSQEAKHHHAFMIGKISGVSRAPENPKRWIINIDSYAEIDIPGQWDGNRNPVSY
                     RKLEDMGIDISKVDFQPLPKEDSLVLNTIKGGDEYDEYEEDSNRVRPINLKEAKAGLAL
                     YFGVNEDDIQITIKG"
                     /name="hypothetical protein"
     gene            1156..1746
                     /locus_tag="D8M16_RS25060"
     CDS             1156..1746
                     /locus_tag="D8M16_RS25060"
                     /inference="COORDINATES: protein motif:HMM:NF019843.4"
                     /GO_function="GO:0005515 - protein binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="WP_023343079.1"
                     /translation="MPSLSVERWLALATAGDSQARFHLGKIFSCGLGVHQDYKQAIYWF
                     QLAAEQGHDDAQFSLGWAYHLGLGGYKDAQKASEWYQKAAEQGHAEAQFQLGRLYAFSD
                     LRNDALAVEWFLCAAKQDHSSAQFKLGWMYHLGMGVEQSDRQAFHWFILAAEQDHAGAQ
                     ASLGEMYKNGWGVEQDDVAAQDWFYRARTMHYP"
                     /name="tetratricopeptide repeat protein"
     gene            1829..2290
                     /locus_tag="D8M16_RS25490"
     CDS             1829..2290
                     /locus_tag="D8M16_RS25490"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_065707752.1"
                     /translation="MVNTYYGYLLGVTLGEWAMGSDVQWQIDNGYLAQVMGDFSTESPS
                     FETPMFGRYFDTFQAAIHVAKAFPGSTLQPSEYEDKTGMLIRHYYVDCHPYYDRLPPEK
                     SDNFQYDEYPSAIHVAACIDQASKELDELLFGGGYGENGEGHASPIILY"
                     /name="hypothetical protein"
     gene            complement(2545..2655)
                     /locus_tag="D8M16_RS25745"
     CDS             complement(2545..2655)
                     /locus_tag="D8M16_RS25745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_048246078.1"
                     /translation="MHDPPVQSDYHAHSGNYQLESNPERHDKIAIGGSHW"
                     /name="hypothetical protein"
     gene            3299..3622
                     /locus_tag="D8M16_RS25080"
     CDS             3299..3622
                     /locus_tag="D8M16_RS25080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_001445156.1"
                     /translation="MLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPARAGKLPSI
                     SPALLRQLAGMGNNLNQIARQVNAGGGSGHDRVQVVAALMAIDAGLERLRHAVLEKGAD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            3831..>4265
                     /locus_tag="D8M16_RS25750"
                     /pseudo=""
     CDS             3831..>4265
                     /locus_tag="D8M16_RS25750"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            4300..4785
                     /locus_tag="D8M16_RS25295"
     CDS             4300..4785
                     /locus_tag="D8M16_RS25295"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_023343076.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESE
                     KRISAAILDHDRKLSSAMSQRTKGMLRMVSQTWLTIVLVSVLLIASNAAILWWQSQQIL
                     DNYVSIREQKSTQAMLSERNSGVQLSTCGEQRRRCVRVNPEAGRFGEDSSWMILAGK"
                     /name="MbeB family mobilization protein"
     gene            4789..5004
                     /locus_tag="D8M16_RS25300"
     CDS             4789..5004
                     /locus_tag="D8M16_RS25300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_102770668.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFAEWRTMCGLMQRENA
                     ALSERVTGLSRQVQSLSDQLRRLSSG"
                     /name="MbeD family mobilization/exclusion protein"
     gene            5275..6189
                     /gene="lpxO"
                     /locus_tag="D8M16_RS25090"
     CDS             5275..6189
                     /gene="lpxO"
                     /locus_tag="D8M16_RS25090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001728425.1"
                     /GO_process="GO:0018193 - peptidyl-amino acid modification
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A hydroxylase LpxO"
                     /protein_id="WP_023343074.1"
                     /translation="MLSLIIIFVFLISVLYAHSRGKIKQKFSRQLFDHSTFMAPINMFM
                     TGFSKLPSKQAWFPPETFPELQPLIDNWQVIRDEAVNLQGHIKSSEKNNDAGFNTFFKR
                     GWKRFYLKWYGDAHPSAERLCPVTTQLVSSIPCIKAAMFAELPSGSHLGKHRDPYAGSV
                     RYHLGLSTPNDERCFIEVDGVRRSWHDGEVIIFDETFVHWAQNDTEQTRIILFCDIERP
                     MKWRWSQKINRWIGGVLMSAAASPNDNNDRTGAINHLFKYVNAIRDAGQKLKKRNRKLY
                     YLIKNGVISLIFVWLAVSVFRWL"
                     /name="lpxO"
     Typing          3012..3195
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="87.234"
                     /coverage="86.0"
                     /evalue="2.69e-52"
     Typing          complement(2546..3029)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.09"
                     /coverage="92.0"
                     /evalue="3.59e-159"
     Typing          3613..5102
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.182"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aatgtatggt tatagataaa aatcgcctgc taaaaaattc tattgaaatc tttttagtaa
       61 gaaaggcccg aatctgacgc tcagcgggcc tttcgtgaag aagtgcggca gtagacagat
      121 ttcatctgat cacgttcatg tgctgctgtg caattaagcg tttctttcgg agctttcacg
      181 gtatttcctt cacgaaaccg gccgccattc actcttgtgc atagccatgc agccgcgcag
      241 aaattatcag gggcgttttt cagggtttgg gccggggaaa ggtgccggaa acggccctca
      301 ggccgcagaa acgcgctgag gggctctcag ggggtgcggc caagaactct tatgttaaat
      361 gcggcggtgt ggcgggttct tacttcacac atcatgttgt cactcagact gagtccagag
      421 agaaaaagcc ctccggccga agtcgagtag tgttttactg gtttttttgc agccctagga
      481 gtaatcgtcg tagtacttcg gatagttatt tgtatatatc ttgtggtttt atgttgagct
      541 cttccgtttt tgatgctaaa aagattgtca ttacgcggtg acttgccttt tttaaaacaa
      601 aaatggagat atctatgctt gatactgact gcattactgt tcttacggcc aggggtatta
      661 ctgagatttt aaatagtgga ggaagccagg cttggagact tgatgctact cacgtagcta
      721 agcttccgta cctggtttgc gtccaaaatt cgaaaaaaga ctggggtagt caggaggcta
      781 agcatcatca tgcatttatg atcgggaaaa tatccggagt aagtcgagcc cctgaaaacc
      841 ctaagcgctg gatcataaat attgattcct atgctgaaat tgatatccct gggcagtggg
      901 acggcaatcg taatccagtc tcctatagaa aacttgaaga catgggaatt gatatctcca
      961 aagtggattt ccaaccccta cctaaagagg attccttagt cttgaatacg ataaaaggcg
     1021 gggatgaata tgatgagtat gaggaggata gcaacagagt taggccgata aatctaaaag
     1081 aggctaaagc agggttggct ttatattttg gtgtgaatga ggatgatatt cagatcacaa
     1141 ttaagggatg accttatgcc gtcactctct gttgagcgct ggttagcact agctactgct
     1201 ggagatagcc aggctagatt tcatctgggt aagatattct catgcggtct tggtgtgcat
     1261 caggattata agcaggctat atactggttt cagcttgcag ctgagcaagg ccatgatgat
     1321 gcccagttta gtcttggttg ggcatatcat ctggggttag gtggatataa agatgctcaa
     1381 aaagcttcgg aatggtatca gaaagctgct gagcaaggtc atgcagaggc tcagtttcag
     1441 cttggaaggc tgtatgcttt ctctgattta agaaatgatg cgttagcagt cgaatggttt
     1501 ctctgtgctg cgaagcaaga ccacagtagt gcccaattta aacttggctg gatgtaccat
     1561 ctcggtatgg gcgttgagca aagtgatagg caggctttcc attggtttat tctggccgct
     1621 gagcaagatc atgcaggagc tcaggcatct cttggtgaga tgtataaaaa tggatggggg
     1681 gtcgagcaag atgatgttgc tgcgcaggat tggttttatc gtgccagaac catgcactat
     1741 ccctaaaccc gccctgtgcg ggttttttta cgcctacgtg taagaggctt gcggagctgg
     1801 ctgctgctga ttcataacat gaagcgttat ggtaaataca tattatggtt atttattagg
     1861 agtaactctg ggagagtggg ctatgggatc agatgttcaa tggcagatag ataatggtta
     1921 tttggcgcaa gttatggggg atttttcgac agagtcgcct agctttgaaa cgccaatgtt
     1981 tgggcgatat tttgatacct ttcaagctgc tatacatgtt gcaaaagctt tcccagggag
     2041 tacgttgcag ccatcagagt acgaagataa aactgggatg ctaatacgtc attattatgt
     2101 ggattgtcat ccgtattatg atcgactccc tcctgaaaaa agcgataatt ttcagtatga
     2161 tgagtaccct tctgctattc atgtggctgc gtgtattgat caagctagca aagaactcga
     2221 cgaactattg tttggtggtg gatatgggga aaatggtgag gggcatgcaa gcccgataat
     2281 tttatattag tgttaggggc ggagggatat tatccaattt gtaaaatgat cttcttgaga
     2341 tcgttttgct ctgcgcgtaa tctcttgctc tgaaaacgaa aaaaccacct ggggaggtgg
     2401 tttttcgaag gttcagtaag ttggggaact tctgaaccgt ggtaacagtc ttgtgcgaga
     2461 cgtgtcacca aatctgtcct tccagtgtag ccgtagtccg gccaccactt caagaactct
     2521 cgatacatct ctcgcacatc ctgcttacca gtggctaccg ccaatggcga ttttgtcgtg
     2581 tctttccggg ttggactcaa gttgatagtt accggaatgg gcgtggtagt cggactgaac
     2641 gggggggtcg tgcatacagt ccagcttgga gcgaactgcc tacccggaac tgagtgtcag
     2701 gcgtggaatg agacaaacgc ggccacataa cagcggaatg acaccggtaa accgaaaggc
     2761 aggaacagga gagcgcacga gggagctacc agggggaaac gcctgggatc tttatagtcc
     2821 tgtcgggttt cgccgccact gatttgagcg tcatatttcg tgatgcttgt caggggggcg
     2881 gagcctatgg aaaaacggca acgccgcatc cttttcccag cccttcctga tttgagcgcc
     2941 atctctttat cccgccattc gtgagccggt aacgcccgcc gcagtctcac ggcaggagcg
     3001 tagcgaccga gtgagcgagg aggcgtcatt tcatcgtccc cagcatttcg cacttacgcc
     3061 ccacagccgc attttttacc tgccacatga agcacttccc tgaactccca cttcgattta
     3121 ccatattaag ccagtataca ctccgctagc gctacgtgac tggttcaggg ctgcgccccg
     3181 aaacccgcta aaaccactga cgcgcctgcg gcttgtccaa caccgcgccg accgggaaaa
     3241 gatttcccgc ccgtcccggc gttatcagga ggccggattg gcagacaaac gcagcaaaat
     3301 gctcacgatg tgggtgaccg aggatgagca ccggcgtctg ctggagcgct gcgacggtaa
     3361 acagcttgca gcctggatgc gccagacgtg cctggacgag aaacccgccc gcgccggcaa
     3421 gcttccctcg atctcgccgg cgctgcttcg tcagcttgcc ggcatgggga acaaccttaa
     3481 ccagattgcc cggcaggtta acgccggtgg tggcagcggg cacgaccgcg tgcaggtcgt
     3541 cgccgcgctg atggccatag atgccggact cgagcggctg cggcatgccg tgctggaaaa
     3601 gggtgcagac gatgatcgtt aagtttcacc cgcgagggcg cggcggtggc gccggtccgg
     3661 tggattatct gctggggaaa gaccgacagc gcgagggcgc caccgttctg caggggaagc
     3721 cggaagaagt cagggagctt atcgacgcct caccctacgc aaaaaagtac acatccggcg
     3781 tcctgtcctt tgccgaaaag gatttaccgc ccgggcagcg cgaaaagctg atggcgagct
     3841 ttgaacgggt tctgatgccc ggactcgaca aagaccagta cagcgtgctg tgggttgagc
     3901 accaggataa ggggcggctg gagctgaact tcctgatccc gaacacggag ctgctgactg
     3961 gcaagcgtct ccagccgtat tacgaccgcg ccgaccgtcc gcgcattgat gcctggcaga
     4021 ccatcgtgaa cggcaggctg gggctgcatg acccgaacgc gcctgaaaac cggcgggcgc
     4081 tggtcacgcc gtccgcgctg ccggaaacga agcaggaagc cgctcaggca attacacggg
     4141 gtttactggc cttggcctcg tccggggagc tgaaaacccg ccaggacgtc actgaggcgc
     4201 tggaaagcgc aggttttgag gtggtacgca ccacaaaaag cagcatcagc attgccgacc
     4261 cggacggggg gcgaaacatc cgactgaagg gagccatcta tgaacagtct tttaacgctg
     4321 gcgaaggact tagagcagaa atcgaaagcg cagcagcaga gtaccggcga gatgctgaaa
     4381 gccgcattca gcgagcacga gaagtctgtc agagcggaac tgagcgaaag cgagaagaga
     4441 atcagcgccg ccatcctcga ccacgaccgg aagctgtcct cagccatgag ccagcgtacg
     4501 aaagggatgc tgcgcatggt cagccagacg tggctgacca tcgtcctggt ctccgtgctg
     4561 ctgatcgcct cgaacgcggc cattctgtgg tggcagagtc agcagatact cgacaattac
     4621 gtgagcatcc gggagcagaa gagcacgcag gcgatgctgt ccgagcggaa cagcggcgtg
     4681 cagctctcga cctgcggcga gcagagacgc cgctgcgtga gggtgaaccc ggaagcggga
     4741 cggttcggag aggattcgag ctggatgata ctggcgggga aatagcacat gacagagctg
     4801 gaaaaacagt tgctgagcgc attagagcag ctacagcagg actactcgaa aaggctggac
     4861 gagtgggaga gcgccttcgc ggaatggcgg acgatgtgtg gtcttatgca acgggagaac
     4921 gcggcgctga gcgagcgcgt cacgggcttg agcaggcagg tgcagagttt gagcgatcag
     4981 ctgcgccggt tgtcgagcgg ctgaatgccg tcgagctgca gcaggtgcgg gaggcgcggc
     5041 atcagaaagc gctggagctc gaacgatcac agcgacagcg ggtatatcac ggtccgtccc
     5101 tttgattcat ttcagcataa ttcctgctga aatcctctct gcctgtgtag tagatatgtt
     5161 ctggtgaagc ttcgattata ctattgataa atagcattta gattataatt taagtgcatt
     5221 cattatgtta tctttaagag atttttatta ttggcgtgaa caaggaatat acacatgctg
     5281 tcacttatta ttatttttgt ttttcttatc agtgtattgt atgcgcattc tcgcggtaaa
     5341 ataaaacaaa agttttccag gcagttattt gatcattcaa catttatggc tccgataaat
     5401 atgtttatga caggcttttc gaagctgccg tcaaagcagg catggtttcc cccagaaaca
     5461 tttcctgaac ttcaaccatt aattgataac tggcaggtta taagagacga agctgttaat
     5521 ttgcaagggc acattaaatc atctgaaaaa aataatgatg caggatttaa tacatttttt
     5581 aagcgcggtt ggaagcgctt ttacctcaag tggtatggag atgctcatcc atcagcggag
     5641 cggctttgcc cagtaacaac ccaactggtt agctctattc cctgtattaa agcagcgatg
     5701 tttgctgaat tgccttcagg aagccattta ggaaagcatc gtgaccctta tgccggttca
     5761 gttcgttatc atttaggatt atcaacacca aatgatgaac ggtgttttat tgaagttgat
     5821 ggtgtacgcc gcagttggca tgatggtgaa gtgataattt ttgacgaaac ttttgttcac
     5881 tgggctcaga atgataccga gcagacacgt attattttgt tctgtgatat agaaaggccg
     5941 atgaagtggc ggtggtcaca aaaaataaat aggtggatag gtggggtgct aatgtcagcg
     6001 gcagcatcac ccaacgataa taatgacagg actggtgcta taaatcattt atttaaatat
     6061 gttaatgcta ttcgtgatgc aggtcagaag ctaaaaaaaa ggaatcgaaa actatattat
     6121 ttgattaaaa atggagttat ttctttgatt tttgtttggc ttgctgtctc agtgtttaga
     6181 tggctgtgat ttgttttttt ctattgagtc atacataaat ttgggtcttc tatgctc
//
