LOCUS       NZ_CP032423             1521 bp    DNA     circular CON 18-JUN-2024
DEFINITION  Escherichia coli strain SCEC020001 plasmid p4_020001, complete
            sequence.
ACCESSION   NZ_CP032423
VERSION     NZ_CP032423.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN06909172
            Assembly: GCF_002165115.2
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1521)
  AUTHORS   Liu,L., Feng,Y. and Zong,Z.
  TITLE     The complete genome of Escherichia coli strain SCEC020001
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1521)
  AUTHORS   Liu,L., Feng,Y. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-SEP-2018) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
REFERENCE   3  (bases 1 to 1521)
  AUTHORS   Liu,L., Feng,Y. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAY-2017) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.6
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology             :: Illumina HiSeq; MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002165115.2-RS_2024_06_18
            Annotation Date                   :: 06/18/2024 01:51:24
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,938
            CDSs (total)                      :: 4,820
            Genes (coding)                    :: 4,552
            CDSs (with protein)               :: 4,552
            Genes (RNA)                       :: 118
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 96 of 268
            Pseudo Genes (incomplete)         :: 174 of 268
            Pseudo Genes (internal stop)      :: 51 of 268
            Pseudo Genes (multiple problems)  :: 45 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP032423.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1521
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="SCEC020001"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="p4_020001"
                     /geo_loc_name="China: Sichuan"
                     /collection_date="2016-06"
     gene            complement(join(660..1521,1..20))
                     /locus_tag="C7V21_RS25335"
     CDS             complement(join(660..1521,1..20))
                     /locus_tag="C7V21_RS25335"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="WP_162919571.1"
                     /translation="MATLKLYHNGLTAGIPPMKNNHTRAKRGEVKGWSKTASRNNTKFL
                     RSVYVPDLTGAAVAFTLTIRDCPPTSDDWKKLREAFIVRLRRAGLVRLHWLTEWQKRGV
                     PHLHGIAYFPDAYEPSTVIWNWLAVAEKYGANIRSQHVVPVTDMQGWFMYLAKHAGRSE
                     THYQRNPEFVPAGWAKTGRVWGYCGDWPIDEPMQLDASHAAFCALRRICRNWRLADARS
                     ANYGKVKDAEKKRRRRIVSARTMLRCNKLGQSACHGVSEWIPLDVSLTAAYWCMSQHPM
                     PEVDDSRHQIEI"
                     /name="replication protein"
ORIGIN
        1 tataatttca gcgtagccaa ggttagatcc tttaccgttc agaattggtt accaagcccc
       61 ggactgttcg cgcagttgcg gggttttctt ttgtccaaag gacagggaaa agatacaccc
      121 aaagaaggga aaaaaacagg agtcgcgtcg agttatgtaa caactaatca agacagccac
      181 tcagaacgct tccagaaaaa ccgatgcagt gcatacatga atacacagtt aaaggggtaa
      241 gtgggccaaa ttggcacaaa tgtgcagtat tacagacact gcacaacgag atcggtaatg
      301 gcgaggatgc gaatcagaac ggcgtgtgcc tcctcgcaga gctgcggggg caatccccgt
      361 cccgattcgt gatcgcacga caaaccctat caccctggac agagttgcaa atctacaaat
      421 aaggcatacc gagcaaaacg aaaatacccc cacggcatca cctgtagggc agggcttacg
      481 cacctacccc acaggcaacg ccttgaaggg ggtaaaaagc ggctttacag ggcgatggct
      541 caccatgtga accatcaccc ataactagga tgaataggga gtgtcgtaca accactaaat
      601 cggaaccctg aagggatccc ccgatttaga agttgacgag tcggcggcgg tgaacgctgt
      661 cagatttcaa tctgatggcg tgaatcgtct acctccggca tggggtgctg agacatgcac
      721 cagtacgcag ccgtcagaga cacatcgagc gggatccact ccgagacacc atggcaggca
      781 gattgcccca gcttgttaca gcgcaacatt gtccgcgcgg agacaatacg gcgacggcgt
      841 ttcttttcag cgtctttcac tttgccataa ttagctgaac gggcatctgc aagacgccag
      901 ttacgacaaa tgcgcctgag agcacagaac gcagcgtgag aggcgtcgag ctgcataggc
      961 tcatcaatgg gccaatcacc acagtaaccc catacacgac ccgttttagc ccatccagca
     1021 gggacaaact cagggttgcg ctgatagtga gtctcagaac ggccagcgtg cttagccagg
     1081 tacataaacc agccctgcat atccgtgaca ggtacaacat gctgagaacg gatatttgca
     1141 ccgtactttt ctgcaacagc aagccaattc caaatgacgg tcgaaggctc ataggcatca
     1201 ggaaagtaag cgatcccatg caaatgggga acgccacgct tttgccattc agtaagccag
     1261 tgaagacgaa ccagaccagc acgacgaagg cgaacgatga aagcctcgcg gagttttttc
     1321 caatcgtcag acgttggagg acaatcgcgg atagtgagcg taaacgccac agcagcgcca
     1381 gtgaggtcag gaacgtatac agagcgaagg aatttagtgt tattgcgaga ggcagtctta
     1441 gaccagccct taacctctcc acgctttgcg cgggtatgat tatttttcat gggcggaatc
     1501 ccagccgtaa gaccgttatg a
//
