LOCUS       NZ_CP032422             1989 bp    DNA     circular CON 18-JUN-2024
DEFINITION  Escherichia coli strain SCEC020001 plasmid p3_020001, complete
            sequence.
ACCESSION   NZ_CP032422
VERSION     NZ_CP032422.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN06909172
            Assembly: GCF_002165115.2
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1989)
  AUTHORS   Liu,L., Feng,Y. and Zong,Z.
  TITLE     The complete genome of Escherichia coli strain SCEC020001
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1989)
  AUTHORS   Liu,L., Feng,Y. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-SEP-2018) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
REFERENCE   3  (bases 1 to 1989)
  AUTHORS   Liu,L., Feng,Y. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAY-2017) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.6
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology             :: Illumina HiSeq; MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002165115.2-RS_2024_06_18
            Annotation Date                   :: 06/18/2024 01:51:24
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,938
            CDSs (total)                      :: 4,820
            Genes (coding)                    :: 4,552
            CDSs (with protein)               :: 4,552
            Genes (RNA)                       :: 118
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 96 of 268
            Pseudo Genes (incomplete)         :: 174 of 268
            Pseudo Genes (internal stop)      :: 51 of 268
            Pseudo Genes (multiple problems)  :: 45 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP032422.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1989
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="SCEC020001"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="p3_020001"
                     /geo_loc_name="China: Sichuan"
                     /collection_date="2016-06"
     gene            join(1805..1989,1..532)
                     /locus_tag="C7V21_RS00045"
                     /old_locus_tag="C7V21_00045"
     CDS             join(1805..1989,1..532)
                     /locus_tag="C7V21_RS00045"
                     /old_locus_tag="C7V21_00045"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_001747339.1"
                     /translation="MWQARFYQSLPRIVADYPDARWMFLTLTVRNCAIGELGETLSRMN
                     MAFQRLKDRKEFRPVQGWIRTTEVTRGSDGSAHPHFHTLMMVPPSMFTRDYVKHARWVE
                     LWRECLRVDYDPSVDVRAVKPRKPKDGESLACATAELVRGAVAETLKYSTKPADMVADP
                     EWFLELTRQTHKRRFVATGGALKDVLKLDQETDADMVIGDDISEGDDDGSRIAFEWKTE
                     VRKYRRSPTKDKTESG"
                     /name="protein rep"
     gene            922..1251
                     /locus_tag="C7V21_RS00050"
                     /old_locus_tag="C7V21_00050"
     CDS             922..1251
                     /locus_tag="C7V21_RS00050"
                     /old_locus_tag="C7V21_00050"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_024191011.1"
                     /translation="MVSFIAQGCAPNPANASRYQIKNLSAFTVPEFVKQKIAGSRAFGV
                     GSDFVIDDGTGKKHWGWWLVSRSEANHLQDGFVVHKISGKACECFVGKTLSGLLFLPPE
                     NQLRF"
                     /name="hypothetical protein"
     Typing          535..616
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="95.181"
                     /coverage="90.0"
                     /evalue="7.3e-29"
     Typing          complement(259..450)
                     /name="ColpVC"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.964"
                     /coverage="100.0"
                     /evalue="6.14e-93"
ORIGIN
        1 ctggatccgc acgacggagg ttactcgagg cagcgacggt agcgcacacc cgcacttcca
       61 taccctgatg atggtgccgc cctcgatgtt cacgcgtgac tatgtcaaac atgcgcgctg
      121 ggttgaattg tggcgggagt gtctgcgggt agattacgac ccgagtgttg atgtccgggc
      181 agtgaaaccc cgcaagccga aggacggcga atcgctggcc tgcgcgaccg ccgaactggt
      241 gcgcggtgcg gtggccgaaa ccctgaaata ttccacgaag ccggctgaca tggtggccga
      301 tcctgaatgg ttcctcgaac tgactcgcca gacgcacaaa cgccgctttg tggcgaccgg
      361 cggcgcactc aaagacgtgc tgaaactcga tcaggaaacc gacgccgaca tggtgatcgg
      421 ggatgatatt tccgagggcg acgatgacgg ttcacgcatc gcgttcgagt ggaaaaccga
      481 ggtcagaaag taccgtcgct caccgacgaa agataaaact gaatcaggct agcggggtgt
      541 cggggtgcac ccctgaccag atggcaattg tgatagcgtc gcgtgtgacg gtattacaat
      601 tgcacatcct gtcccgaaaa ttctcgccgt atctcggttt tttgagcgta gcaggccgca
      661 ggacaaacga aaccaatgca ggatagacaa tcatggtcag aacgttaaca cgttgcccag
      721 attgtccgcc tgccgcaggc tctgttttta attttcggtg aaaacaaaaa ttaaaattct
      781 ggattttgga aagagtgtgt ttttaaaaac acgcgttttt gatttttgaa aagagcctgc
      841 ggcaggcaca ggttgataat ccgatgacaa atgcccaatt tgaaaattgg taacaattga
      901 catcggtttg tctatcctgc attggtttcg ttcatcgctc agggctgcgc cccgaacccc
      961 gccaacgcct cacgctatca gattaaaaat ctgtcagcgt tcaccgttcc cgaattcgtc
     1021 aaacagaaaa tcgcggggtc ccgcgccttt ggcgtgggtt ccgattttgt gattgacgac
     1081 ggcacgggaa aaaaacactg gggatggtgg ttggtgtcac gcagtgaggc caaccacctg
     1141 caagacggtt ttgttgttca caaaatcagt ggaaaagcct gtgagtgttt tgtcgggaaa
     1201 acgctttcgg ggttgttgtt tttgccgcca gaaaatcagc tcagattttg agcaaatctc
     1261 gcgcgcgatc cttgtattta tacttaaggg ataactctgg ggataaaaaa tagctacagg
     1321 ccttgtgtgg caaggctttg cggcttattt gcgttgtaga aggttttaga agtattctgt
     1381 cgaaagtggt cgcattaaaa gcgacccccg caactgcgct aacagttcgg gggtgtgacc
     1441 acctgtagga attcgaattg gtgacacgac aacacaggtg atatggcaat gataacacgt
     1501 cagatgagcc atcgcccaca atcgggtggt gtgcatggaa aatcctgaac tccagaacct
     1561 cacggactat tccccctcag atgcgccatg ggacgctcac agaagcgctt ctgacgacgt
     1621 gggagggatt tacctgcttg cagctgaata tgagcgttac ggggcgcgta tggcctcctg
     1681 tggcggttta ctgcgttttg gatggtctac cctcaaggaa accggagaaa cgcgactgag
     1741 gctgcgtgag gcgcatttct gccgtgtccg gcactgtcct gtctgccagt ggaggcgctc
     1801 cctgatgtgg caagcccgtt tttatcagtc tttgccgcgt attgtggctg actatccaga
     1861 cgcacgatgg atgtttctga ccctgaccgt gcgcaactgt gcgatagggg agctgggcga
     1921 gacactgagc cgaatgaaca tggccttcca gcgactgaag gatcgcaaag agtttcgacc
     1981 tgttcaggg
//
