LOCUS       NZ_CP032421             2444 bp    DNA     circular CON 18-JUN-2024
DEFINITION  Escherichia coli strain SCEC020001 plasmid p2_020001, complete
            sequence.
ACCESSION   NZ_CP032421
VERSION     NZ_CP032421.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN06909172
            Assembly: GCF_002165115.2
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2444)
  AUTHORS   Liu,L., Feng,Y. and Zong,Z.
  TITLE     The complete genome of Escherichia coli strain SCEC020001
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2444)
  AUTHORS   Liu,L., Feng,Y. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-SEP-2018) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
REFERENCE   3  (bases 1 to 2444)
  AUTHORS   Liu,L., Feng,Y. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAY-2017) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.6
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology             :: Illumina HiSeq; MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_002165115.2-RS_2024_06_18
            Annotation Date                   :: 06/18/2024 01:51:24
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,938
            CDSs (total)                      :: 4,820
            Genes (coding)                    :: 4,552
            CDSs (with protein)               :: 4,552
            Genes (RNA)                       :: 118
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 96 of 268
            Pseudo Genes (incomplete)         :: 174 of 268
            Pseudo Genes (internal stop)      :: 51 of 268
            Pseudo Genes (multiple problems)  :: 45 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP032421.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2444
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="SCEC020001"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="p2_020001"
                     /geo_loc_name="China: Sichuan"
                     /collection_date="2016-06"
     gene            join(2187..2444,1..711)
                     /gene="mobV"
                     /locus_tag="C7V21_RS00030"
                     /old_locus_tag="C7V21_00030"
     CDS             join(2187..2444,1..711)
                     /gene="mobV"
                     /locus_tag="C7V21_RS00030"
                     /old_locus_tag="C7V21_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000024818.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_000024818.1"
                     /translation="MANYAIMRCKKLTGMGSVASALQHCYRERETPNANAERTPENYCS
                     VSKSTDQAMGRVRELLPEKRRKDAVLAVEYVMTASPEWWKEATPRQQAEFFARSEQWLE
                     KKYGKDRVVAAVVHRDEATPHLSAFVVPLTQDGRLSAKEFIGGRSKMREDQSTYAESVK
                     KLGLERGIEGSRATHQTVQHYYESINRGTRSQVSISPEALEPRVLRKGIFTKDVEDQAA
                     IAKRLSHAVNDGFAGTIAMASQSAQNAKRARELQKTMDAQQKRLQSVTEPFKGLSREQM
                     TEILMMAQRFKQQNQEKEKQQRIEREKQRQTRSRGMGGMER"
                     /name="mobV"
     gene            complement(781..1011)
                     /locus_tag="C7V21_RS00035"
                     /old_locus_tag="C7V21_00035"
     CDS             complement(781..1011)
                     /locus_tag="C7V21_RS00035"
                     /old_locus_tag="C7V21_00035"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_124788667.1"
                     /translation="MMNAEDKLFKNVDRLMLHDSLKKNEVLTVWERTFLSSVYSIYRRT
                     SGNKYSTKGLSPKQKNAAFSILKKHNYHLND"
                     /name="hypothetical protein"
     gene            complement(998..1480)
                     /locus_tag="C7V21_RS00040"
                     /old_locus_tag="C7V21_00040"
     CDS             complement(998..1480)
                     /locus_tag="C7V21_RS00040"
                     /old_locus_tag="C7V21_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001565850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_223375251.1"
                     /translation="MNTKKISRLERHTYMDENGEVKQDSKIETFSIPAEPPYVKLYIAD
                     IAMIHGLSKSQNDVLLQLAMLIGWDGFVSVSKTRFEKTIQPRVHIEYQTFKNIIAALVD
                     KQIFFRAGRGELEANPFLFARGDWPSIYERRKKIELKISYDTNGRTVQTKVMDDEC"
                     /name="hypothetical protein"
     Typing          2179..2444
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.011"
                     /coverage="96.0"
                     /evalue="1.02e-47"
ORIGIN
        1 gccacaccac ggcagcaggc ggagtttttc gcccgctctg agcaatggct ggagaagaaa
       61 tacgggaagg atcgtgttgt ggcggcggtg gttcaccggg acgaggccac cccgcacctg
      121 tctgcgttcg tggtgccatt gacgcaggat ggacggctgt ccgcgaaaga gtttattggc
      181 gggcggtcaa agatgcgtga agatcagagc acctacgctg aatcagtaaa aaaactgggt
      241 cttgagcgcg gcatcgaggg aagccgcgcc acgcatcaga cagttcagca ctactacgaa
      301 tcgataaacc gtggcacccg tagccaggta tcgatctcgc cggaggcgct ggagccgcgt
      361 gtattgcgga agggcatttt caccaaagac gtggaagatc aggcagccat agccaaacgt
      421 ctaagccacg cagtgaacga tggctttgca ggaaccatcg ctatggcctc tcagagcgcc
      481 cagaacgcaa aaagagcgcg tgagctacag aagactatgg atgcgcagca gaaacgccta
      541 cagagcgtca cagagccttt taaggggctt tccagagagc agatgaccga aatcctgatg
      601 atggctcagc ggtttaagca gcaaaaccag gaaaaggaaa aacagcagcg tattgaacgt
      661 gaaaaacagc gacagacgcg atcgcgtggc atgggtggta tggagcgtta gttttgatgc
      721 gatgcaggat agggctaacg ccgcaaagtg acgtcatttg cggcattcac cacgaaaaaa
      781 ttaatcattc agatggtaat tatgtttttt taaaatagaa aaagctgcgt ttttctgctt
      841 tggtgacaaa cccttagttg agtatttatt accgctagtt ctacgatata tgctgtaaac
      901 actgcttaaa aaagtacgct cccaaacagt cagaacctca ttctttttta gtgaatcatg
      961 cagcattagc ctgtctacat ttttaaaaag tttatcttca gcattcatca tccattacct
     1021 tcgtctgcac cgttcttcca ttggtgtcat aactgatctt taactcaatt ttctttcttc
     1081 tttcgtaaat tgatggccag tctccccgtg caaaaagaaa ggggttagct tcaagttccc
     1141 cacgccctgc ccggaagaat atttgtttat ctacaagagc cgcaattatg tttttaaagg
     1201 tctgatattc aatatgaacc ctgggctgta tggttttttc aaatctagtt tttgatacag
     1261 acacaaaccc atcccatcca atcagcattg ccaattgtaa aagcacatca ttttgagatt
     1321 ttgataaacc atgaatcatc gcaatgtctg caatgtatag cttgacgtag ggaggttcgg
     1381 ctgggatgga aaaggtctcg atcttagagt cttgttttac ctcaccgttt tcatccatgt
     1441 atgtatgccg ttcaagccta gaaatctttt tcgtattcac tccagcccca cgtttcgcca
     1501 tgatttagta ctcgcattta agtactaaag agacatacag tcaagtcctt tgtatcaagt
     1561 acgattagta cttgaggtga gtacgattag tccttgaggt gagtacgatt agtacttgag
     1621 gtgagtacga ttggtacttg aggtgagtac gattcttcgc tctagccctt ggtacaagcg
     1681 ggttcccata tctatatcta tatattaagg gggaaaataa gggctgaaaa acggcatttt
     1741 tagacactga aaaactgcct ttttttagca aggagtgtac gcatttctat gtacggggag
     1801 tggaaattcc gcttcctcgc tttggctgtt cgttcctcgt cgcgttgctc ctgcggtacg
     1861 cctcagccaa agctgcggcg agcggttcct gctctttcaa agacgatttc attgcctgat
     1921 ttcgtcaagg gcgagcgtag cgagtgccat ttacccttga cgacactccg gataaaacag
     1981 gcttggcagt ggtggggggt ggcgcgaagc gacgccttcc acctgccaga ccgccgttga
     2041 ttttgacctt ctggcagctt gctgcccgaa cgattttgcg tagcaaagtc tagtgagttt
     2101 acactatcat cttgatagtg gcccgccgga ggtatcgcaa tgcgctcccc cgcctggctg
     2161 aatgaatgcc ggaatcaggg gggaacatgg cgaactacgc gataatgcgc tgcaaaaagc
     2221 tgacgggaat gggaagcgtg gccagcgcgt tgcagcactg ctaccgagag cgtgagacgc
     2281 cgaacgctaa cgctgaacgg acaccagaaa actactgctc tgtgtccaaa tcaacggatc
     2341 aggcaatggg gcgtgtgcgg gagctgctgc cggaaaaacg ccgtaaagat gcggtactgg
     2401 ccgttgaata cgttatgaca gcaagccctg agtggtggaa agag
//
