LOCUS       NZ_CP032277             5934 bp    DNA     circular CON 05-JAN-2024
DEFINITION  Acinetobacter sp. WCHAc010034 plasmid p9_010034, complete sequence.
ACCESSION   NZ_CP032277
VERSION     NZ_CP032277.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN05356835
            Assembly: GCF_001696615.3
KEYWORDS    RefSeq.
SOURCE      Acinetobacter sp. WCHAc010034
  ORGANISM  Acinetobacter sp. WCHAc010034
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter.
REFERENCE   1  (bases 1 to 5934)
  AUTHORS   Hu,Y., Long,H., Feng,Y. and Zong,Z.
  TITLE     The complete genome of Acinetobacter sp. strain WCHAc010034
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5934)
  AUTHORS   Hu,Y., Long,H., Feng,Y. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2018) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
REFERENCE   3  (bases 1 to 5934)
  AUTHORS   Hu,Y., Feng,Y. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAR-2017) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.6
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology             :: Illumina HiSeq; MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/04/2024 21:32:37
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,697
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 112
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 21 of 68
            Pseudo Genes (incomplete)         :: 53 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 16 of 68
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP032277.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5934
                     /organism="Acinetobacter sp. WCHAc010034"
                     /mol_type="genomic DNA"
                     /strain="WCHAc010034"
                     /isolation_source="hospital sewage"
                     /db_xref="taxon:1879049"
                     /plasmid="p9_010034"
                     /geo_loc_name="China"
                     /collection_date="2015-10-31"
     gene            complement(91..561)
                     /locus_tag="BEN74_RS00945"
                     /old_locus_tag="BEN74_00940"
     CDS             complement(91..561)
                     /locus_tag="BEN74_RS00945"
                     /old_locus_tag="BEN74_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004870960.1"
                     /translation="MSNQNDLDDQLYILLASMKEYREAIADDNKRLEAFYKEVASGVLN
                     KTEKHLKNANQKQIDALNNSIRELNNATNQLDWRFMAIYASAFVSLLIVFFLALFLYVP
                     SMDEIKQRRADVAWLEQKYSLDIKNCNGKSCVRIMKNDCHGANKDYCVIDPK"
                     /name="hypothetical protein"
     gene            complement(558..1529)
                     /gene="mobQ"
                     /locus_tag="BEN74_RS00950"
                     /old_locus_tag="BEN74_00945"
     CDS             complement(558..1529)
                     /gene="mobQ"
                     /locus_tag="BEN74_RS00950"
                     /old_locus_tag="BEN74_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004917616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_004897098.1"
                     /translation="MAIYHCSTKTVNRSSGRTAVASSAYRSGEKLKDERTGLTHDFTRK
                     DGVAHSEIVSNLDIEIDRSELWNLAEQSENRKDARTAREWVIALPDELDADQRKDLAKE
                     FAQSLVDRYGVIADLAIHEPSKGGNDKNHHAHIMLTTRKAELDPDNKLTLTTKTDIELS
                     NAKRKSLNMGTTQDDIKQIRETWADLANHALERAGYREKIDHRSYADQNNGLQATIHEG
                     TSVTQLRRQGIDTEISRYNDHVKQHNAQHLKQQQQRTDSVLQHGLNRAEQGFEQWQKNQ
                     EAKRLEQERQAEIQRQQKLEQQQAERANRKASQDLDQGGMYR"
                     /name="mobQ"
     gene            1725..2009
                     /locus_tag="BEN74_RS00955"
                     /old_locus_tag="BEN74_00950"
     CDS             1725..2009
                     /locus_tag="BEN74_RS00955"
                     /old_locus_tag="BEN74_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004856445.1"
                     /translation="MTKSAENIEKKIEAQLEKLKQLKAQKQAIDAREKTKQKEQERKDD
                     TRRKILLGSYLIKKMQSNEANKEKILAELNEYLTENRDRQLFDLPDIEA"
                     /name="hypothetical protein"
     gene            complement(2006..2302)
                     /locus_tag="BEN74_RS00960"
                     /old_locus_tag="BEN74_00955"
     CDS             complement(2006..2302)
                     /locus_tag="BEN74_RS00960"
                     /old_locus_tag="BEN74_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004856442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004856442.1"
                     /translation="MIKLNNLSTDLKHVTVEYLDIVNYEIARENICGYIFLLSRISKNA
                     EPTKKMQMESKIQDLIYYRDNLQIEDKDNIQKVLNTLIPEYQAEQNNQTAKKN"
                     /name="hypothetical protein"
     gene            complement(2299..3222)
                     /gene="repM"
                     /locus_tag="BEN74_RS00965"
                     /old_locus_tag="BEN74_00960"
     CDS             complement(2299..3222)
                     /gene="repM"
                     /locus_tag="BEN74_RS00965"
                     /old_locus_tag="BEN74_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004734284.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepM"
                     /protein_id="WP_004856439.1"
                     /translation="MKKELVVKDNALINASYNLDLSEQRLILLAILEARQSNTPNDKDL
                     TIHAESYINHFNVHRNTAYKVLKDACKSLFDRRFSYQKLTQKGNIENVISRWVQRISYV
                     ENEALVRIKFSDDVVPLITNLEKHFTSYELEQVSSLTSVYAIRLYELLIAWRSTGKVTM
                     VELEELRLKLGIEPNEYKRMGQFKEKVLHLAIDQINEYTDIKAEYEQHKRGRSIIGFSF
                     KFKQKSQPKKLDSKRDPNTPDFFVKMTDAQRHLFANKMSEMPEMGKYSQGTESYQQFAI
                     RIADMLLEPEKFRELYPILEKSGFQP"
                     /name="repM"
     gene            complement(3932..5104)
                     /gene="blaMCA"
                     /locus_tag="BEN74_RS00970"
                     /old_locus_tag="BEN74_00965"
     ARG             complement(3936..5104)
                     /name="blaMCA"
                     /gene="blaMCA"
                     /gene_name="MCA family class C beta-lactamase"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="99.23"
                     /coverage="100.0"
     gene            5343..5636
                     /locus_tag="BEN74_RS00975"
                     /old_locus_tag="BEN74_00970"
     CDS             5343..5636
                     /locus_tag="BEN74_RS00975"
                     /old_locus_tag="BEN74_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004973743.1"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="WP_004870963.1"
                     /translation="MSAVNFNMRLEAELKEQVTPILEDYGLTMPQAFKLFLNQIVKTKA
                     IPLSFDYAREPALTPKAAAKLLQSLKEIENGEYTEYETVEEAIKAMSEEAHG"
                     /name="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
     gene            5629..5910
                     /locus_tag="BEN74_RS00980"
                     /old_locus_tag="BEN74_00975"
     CDS             5629..5910
                     /locus_tag="BEN74_RS00980"
                     /old_locus_tag="BEN74_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001129137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YafQ family toxin"
                     /protein_id="WP_001129137.1"
                     /translation="MAKRKIIVTSSFKRDIKRRYLELVTAEWAEVLDCLVANNPLPEKY
                     LDHPLKGGAEFKNCRDCHVKPDLVLIYRLVGDDILELHQLDSHSEIFG"
                     /name="type II toxin-antitoxin system YafQ family toxin"
     Typing          complement(2309..3222)
                     /name="rep_cluster_1280"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.836"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(562..1529)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ggctttagcc atgtttcaaa gtcgggcaac tttgcgactg ctgtcattga ttcggtgatg
       61 gcaagagccg agcaaagtac tgtctaatca ttattttggg tcaatcacac aataatcctt
      121 attcgctccg tgacagtcat ttttcatgat gcgaacgcaa gacttcccat tgcaattttt
      181 aatatctagg ctgtattttt gttctagcca tgcaacgtct gctctacgct gtttgatttc
      241 gtccatgctc ggcacatata aaaataatgc taaaaagaaa acgatcagca agctaacaaa
      301 agcactggcg tagattgcca tgaatcgcca gtcaagttga ttggttgcat tgtttaattc
      361 tcgaatactg ttgttgagtg cgtctatttg cttctgattg gcgtttttta ggtgtttttc
      421 ggtcttattg agtactccgc tagccacttc cttgtaaaac gcttctaatc gcttgttatc
      481 gtctgctatg gcttctcgat actctttcat agatgccagt aaaatataga gctggtcatc
      541 taggtcgttt tggttgctca tcggtacatt ccgccctgat ctaagtcttg tgatgcttta
      601 cggttggctc gctcggcttg ctgttgctct agtttttgtt gtcgttgtat ttcggcttgg
      661 cgttcttgtt ctaggcgttt agcttcttgg tttttctgcc attgctcgaa gccttgctcg
      721 gcacggttta aaccgtgctg taaaacgctg tctgtgcgct gttgctgttg tttgaggtgt
      781 tgggcgttgt gttgcttcac atggtcgtta taacggctga tttcggtgtc tatgccttgt
      841 ctacggagtt gagtgaccga agtcccctca tgaatggtgg cttgtagtcc attgttctga
      901 tcggcatagc tgcggtggtc tattttttct cggtagcctg ctcgctccag tgcgtgattg
      961 gctaagtccg cccatgtttc ccgaatctgt ttgatgtcgt cttgggttgt tcccatattt
     1021 aggcttttgc gtttggcatt acttaactca atatcggttt ttgtcgtcaa agtgagttta
     1081 ttgtcagggt ctaattcagc ttttcgggtg gtcagcatga tatgggcgtg atggttttta
     1141 tcatttccgc ctttgctggg ttcgtgaatg gctaaatctg caatcacgcc ataacgatca
     1201 acaagcgatt gggcaaactc ttttgccaag tccttgcgct gatctgcatc cagttcatca
     1261 ggcagggcaa tcacccattc tctagcggtt cgagcatctt tgcggttttc ggactgttcc
     1321 gccaagttcc acaattcgct acggtctatt tctatatcaa ggttagacac aatttcgcta
     1381 tgggcgacac cgtcctttct cgtaaagtcg tgggttagtc ctgttcgttc atctttgagt
     1441 ttttcccctg aacggtaggc agacgatgca accgcagttc gtcccgaact tcgattcact
     1501 gtttttgttg agcaatgata aatcgccatt ttttctttcg cctttttaaa ttgcgtagca
     1561 attttttggg gttaagggga ttccccttac gcaacgatga cttgaatgaa attcaagtcg
     1621 taagtgcgca tttcatgaaa aacacgatag catatagccc gcttgaattt caattcatag
     1681 ggttttatgc ttgagtggtt ttctatatcc cgaaattaac cgttatgaca aaatctgctg
     1741 aaaatatcga aaagaaaatt gaagcccaat tagaaaagtt gaaacagtta aaagctcaaa
     1801 aacaggctat tgatgcaaga gaaaaaacca agcaaaagga acaagaaaga aaggacgata
     1861 caagacgtaa aattttgctc ggttcttact taattaaaaa gatgcaatct aatgaagcca
     1921 ataaagaaaa aatactcgct gaactcaacg aatatttaac agaaaataga gatagacagc
     1981 tttttgattt gcccgatatt gaagcctaat ttttcttagc tgtttggttg ttctgttcgg
     2041 cttgatactc gggaatgagc gtatttaaaa ccttttgaat attgtctttg tcctctatct
     2101 gcaaattatc tcgatagtaa attaagtcct gtattttgct ttccatctgc atttttttgg
     2161 tgggttctgc atttttggat attcgagata ataggaagat ataaccgcat atattttccc
     2221 tcgcaatttc ataatttacg atgtcgaggt attcaacggt cacatgtttt agatcagttg
     2281 ataggttgtt taatttaatc atggctgaaa tcctgatttt tctaatattg gataaagctc
     2341 tctgaatttt tcaggctcta aaagcatatc tgctatacgg atagcaaatt gttggtagct
     2401 ttctgtacct tgtgaatatt tccccatttc aggcatttct gacattttat tggcaaataa
     2461 atggcgttgt gcatcggtca ttttgacaaa aaagtcaggt gtgtttgggt cacgcttaga
     2521 atctaatttt ttcggctgtg atttttgctt aaacttaaat gaaaaaccaa taattgaacg
     2581 accacgtttg tgttgttcat actctgcttt tatatctgtg tactcattaa tttgatcaat
     2641 ggcaaggtgt aaaacttttt ctttaaattg ccccattctc ttgtattcgt tgggttctat
     2701 gcctaatttt aatcttagct cttctagctc taccatagtc actttgcctg tactacgcca
     2761 tgcgataagc aactcataca agcgtatagc gtaaacacta gttaaactgc tgacttgctc
     2821 tagttcataa ctggtgaagt gtttttctaa atttgtaatc aacggtacaa catcatcaga
     2881 aaacttaata cgaacaagag cttcattttc tacataagat attcgttgta cccatcgact
     2941 tattacattc tcaatgttgc ccttttgagt cagcttttga tagctaaatc tacgatcaaa
     3001 taggcttttg caagcatctt taagaacttt ataggctgta tttctatgaa cattgaaatg
     3061 gttgatatag ctttcagcat gaatcgtcaa atctttatca ttgggtgtat ttgattgcct
     3121 agcttcaagt atggctaaca gtattaaacg ttgttctgat aggtctaaat tataactggc
     3181 atttattagt gcattatctt taacgactag ttcctttttc ataagcacac atgtttttta
     3241 aatatataag tacactctat tttattgagt gtcactttgc aagataaagg tgtacttatt
     3301 gcaagataaa ggtgtactta ttgcaagata aaggtgtact tattgcaaga taaaggtgta
     3361 cttattgcta tttttattct tatatatcaa ttaattgtag aggctaaaaa ttaaaaaatt
     3421 aaaaaatatt aaaaagaggg gagtgcttaa aactgcccta tgtactcgct aaagctcgta
     3481 ctctattgaa gctcgttcct cgcttcatgg ggcagttttt ggttaatcct gtttatgaaa
     3541 tctaaaagca aaagcattgg taatgtattc gcttcgctca tactttttga aaagcaaaag
     3601 ctccaaagtt cgcacccatt cgggatgctc tgtaactacc gtttaaaatc gaaagggagc
     3661 ttgtaatcag agaaaaagcg aggtcgaaat tttgatgatg ctcaatcgct cgtagacact
     3721 cgctcggttc ttattatcta ggttttaatt atttactgga gctaatcaaa gttgtgaaac
     3781 attgatgaaa tattagtcgt ggaacgtgaa gaatcataaa aaaagcccga gcatgacgtt
     3841 tcgggctatt aagtattgac tataacattg aaagataatg aaaatatcta agcatttcgg
     3901 ataaggtgta ttatgttaat tttagagaaa gttaattatc tttcaatgtc tcaaaaacat
     3961 tatatgaagc cttaatacga tctacatttg gtatcttttt attcattaac ataaccaatc
     4021 caaatccttc ttctggaata aataaaacat acgccccaaa gccattagtc gaacctgttt
     4081 tatgaaaaac tttagatttt ggttgcgata attcttttac aacaggattt gagcccaata
     4141 agatttgttt tgaattacta gcctgtagaa tttcagaagt agtgggataa gaaaacattt
     4201 cccatccaag tgcttgtgtc ataccgctat cagaaacttt aaaatatcct ttgtgtgtat
     4261 ccagtatagc ttttttcata gcagggctat ttgtatctac attaagattc gaatttacaa
     4321 acttaagcat atctgggagt gttgatttaa cgccatatgc ttcatccgac aatggaccag
     4381 gattaactcg aattggctta ttattttcat cataaccaaa agcatagttc gttttttgtg
     4441 cttctggaac attgacgtat gtatgtttca aattaagttg aggaaaaaca gtcttctcta
     4501 ataatgaaga gaaaggaaca ttcatcgatt ttgcagttag atacccaaaa agtcctatac
     4561 ttggatttga gtattcacga taagtaccag gaggattctt tactttccaa tttttgaaat
     4621 actctaatat ttgcttatca gttttgatat tatctggaaa ttgtaatggc aaattgccac
     4681 ttgtataagt taataactct aataaattta ccttatcaat ttctgaattt tttaaagcag
     4741 ggacgtattt actaggatga tcgttgaacg atattttacc ttgattattg gcatatgttc
     4801 ctgaaatagc agtaaaagtt ttacttaaag aacctaactc aaaaagtgtc tgactattta
     4861 cgctttcatt tgtatcttta gatcggacac cataatattt ttcataactt ttaccgttgt
     4921 aaattacgcc tatagccata ccagctacac catactcatc tataagtggc ttaaatgatt
     4981 gggtaacaac tttttcaata tttttctcat tttgactgac ttcacttgca tttaaattca
     5041 taccaaaacc gataaccatc gtaatgaagc ttgcttgaaa aaaatatttt tgattaaaaa
     5101 acattaggtt aaatttcttt aaaaaagatg gcacatataa tctcatatac taaataacat
     5161 atgtattaat ttttgttaaa taacttctta tataaaaatc atctagccca acagctattt
     5221 aacataatgg ctgttatacg aaattcagtt gtcagaaatc atttattaca tcaaaagtaa
     5281 tgaaaaagta aagaatatga cttatattat ctttacttta atggtttttg agtgagggaa
     5341 tcatgtccgc agtcaatttc aatatgcgct tagaagcaga gttaaaagaa caagttactc
     5401 ctattttgga agactatggt ttgactatgc ctcaagcatt caaactgttc ttgaatcaaa
     5461 tcgttaaaac taaagcgatt cctttgtctt ttgactatgc acgagaacct gctttaactc
     5521 ctaaagcagc agccaagtta cttcaatctt taaaagagat tgaaaatggc gagtacaccg
     5581 agtacgaaac ggtagaagaa gcgattaaag ctatgtcgga agaagcacat ggctaaacgt
     5641 aagattatcg taaccagttc ttttaaacgt gatattaaaa gacgttattt agaactggtt
     5701 acggctgaat gggcagaagt attagattgt ttagtcgcaa ataatccttt gcctgaaaag
     5761 tatttagatc atcccttgaa aggtggtgca gagtttaaaa attgtcgtga ttgtcatgtg
     5821 aagcctgatt tagtcttgat ctataggctt gttggtgacg atattttaga actgcatcag
     5881 ttggatagcc attccgaaat ttttggatga ctatcgctcg atgctttgaa agat
//
