LOCUS       NZ_CP032275            10090 bp    DNA     circular CON 05-JAN-2024
DEFINITION  Acinetobacter sp. WCHAc010034 plasmid p7_010034, complete sequence.
ACCESSION   NZ_CP032275
VERSION     NZ_CP032275.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN05356835
            Assembly: GCF_001696615.3
KEYWORDS    RefSeq.
SOURCE      Acinetobacter sp. WCHAc010034
  ORGANISM  Acinetobacter sp. WCHAc010034
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter.
REFERENCE   1  (bases 1 to 10090)
  AUTHORS   Hu,Y., Long,H., Feng,Y. and Zong,Z.
  TITLE     The complete genome of Acinetobacter sp. strain WCHAc010034
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10090)
  AUTHORS   Hu,Y., Long,H., Feng,Y. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2018) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
REFERENCE   3  (bases 1 to 10090)
  AUTHORS   Hu,Y., Feng,Y. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAR-2017) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.6
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology             :: Illumina HiSeq; MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/04/2024 21:32:37
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,697
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 112
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 21 of 68
            Pseudo Genes (incomplete)         :: 53 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 16 of 68
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP032275.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10090
                     /organism="Acinetobacter sp. WCHAc010034"
                     /mol_type="genomic DNA"
                     /strain="WCHAc010034"
                     /isolation_source="hospital sewage"
                     /db_xref="taxon:1879049"
                     /plasmid="p7_010034"
                     /geo_loc_name="China"
                     /collection_date="2015-10-31"
     gene            complement(join(9653..10090,1..495))
                     /locus_tag="BEN74_RS00810"
                     /old_locus_tag="BEN74_00805"
     CDS             complement(join(9653..10090,1..495))
                     /locus_tag="BEN74_RS00810"
                     /old_locus_tag="BEN74_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000742756.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="WP_086374392.1"
                     /translation="MKKPTHKIYRTTNWPAYNRALMSRGNIAIWFDPATQWYAPSKGKQ
                     GRNQTYSDAAIQCCLMIKSLFRLSVCMVTGFVQSLIKLCGLDWTALDYSTIRRRQKHID
                     IQISYEKSCNGLHLLVDSTGMKFLGEGEWKRKKHGAEYRRQWRKLHIGIDAKTLQIRAI
                     QLTTNNVSDSQVLGDLLNQIPQDEQIDSVYTDGAYDTKQCRQVIADRQAHAVIPPRKNA
                     KPWKDTKSSSLERNELLRTVKHLGRTLWKKWSGYHRRSLVETKMHCIKLLGDKLMARSF
                     PSQVNEIHARVAVLNRFTELGRPLTQVTP"
                     /name="IS5 family transposase"
     gene            complement(<545..757)
                     /gene="glmM"
                     /locus_tag="BEN74_RS00815"
                     /old_locus_tag="BEN74_00810"
                     /pseudo=""
     CDS             complement(<545..757)
                     /gene="glmM"
                     /locus_tag="BEN74_RS00815"
                     /old_locus_tag="BEN74_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008891390.1"
                     /note="catalyzes the conversion of glucosamine-6-phosphate
                     to glucosamine-1-phosphate; incomplete; partial in the
                     middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /name="glmM"
     gene            complement(844..1659)
                     /gene="sul2"
                     /locus_tag="BEN74_RS00820"
                     /old_locus_tag="BEN74_00815"
     ARG             complement(848..1659)
                     /name="sul2"
                     /gene="sul2"
                     /gene_name="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /tool="amrfinderplus"
                     /drug_class="SULFONAMIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            complement(1998..3281)
                     /locus_tag="BEN74_RS00830"
                     /old_locus_tag="BEN74_00825"
     CDS             complement(1998..3281)
                     /locus_tag="BEN74_RS00830"
                     /old_locus_tag="BEN74_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005175445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_068911102.1"
                     /translation="MFYCNLEHTAKKAIKTRSGKIKDGATAKNRFHYITRTSHFKSYKE
                     SVSEHIEFVKSGNLPSFAEGKPAEFWQAADVYERSNGRTCSSLVVALPKELNQAQRIEL
                     AEAFITEFADRYRFPFSCAIHNHAGLIAGIEQPHLHLMYSERHMDGIDRSAEQFFKRYN
                     PKDPQKGGAQKLTADVLGMGKAQLQLYRQKTEELINDSLMRYAPMKQVEIRGIQIEVPS
                     RVSCLSHRDYNKKYGTQLQDVPVMNKAVRFACENEPELWAKQQHMIEEIQRIRAENRYE
                     QYRSFYEAELKNRKEIEELSKQAETPRTRQQWYAREKLLFLQQNMLQTLLKDPQTTEPI
                     TLIQHDLQRYIDLLNTESFDRLEKLGYLRDLGQNLEGSVIQIAPIDEELAFIQRQVIER
                     VPLKMREKAVTSEKVRNHDQDNDFSPGF"
                     /name="MobA/MobL family protein"
     gene            3504..4259
                     /locus_tag="BEN74_RS00835"
                     /old_locus_tag="BEN74_00830"
     CDS             3504..4259
                     /locus_tag="BEN74_RS00835"
                     /old_locus_tag="BEN74_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004787789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_005175443.1"
                     /translation="MAARGRPAKILTSNDLMTLQQFLKNLPFLKKNQQQALTLLQQANG
                     IFNDKQLNLLKAADRDKNQFLKRQALIEQIKLKDKNQQPLLANETEILALLTQEMDQDN
                     FFRLDRALESYQKIEKAALENRIRLENEHKREILQKSHKELTDAQKKRNAENQLKYALG
                     GAVLAAWNKLNLSTENIDPEKVKNTIVNNQNFFRMVSNTTLYQYIHPRTENYFRSRELF
                     IKVIEGLCEYDDHGTELYIFEADSHLKPQ"
                     /name="hypothetical protein"
     gene            4911..5810
                     /gene="repM"
                     /locus_tag="BEN74_RS00840"
                     /old_locus_tag="BEN74_00835"
     CDS             4911..5810
                     /gene="repM"
                     /locus_tag="BEN74_RS00840"
                     /old_locus_tag="BEN74_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016650500.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepM"
                     /protein_id="WP_004763517.1"
                     /translation="MMSTSKKELVVKSNQVIEASYQLSSTEQRIVLAAISKISRAEDIT
                     DDEIYRVTIDDLKKLGVHEKTAYRDLKDGVNRLYDRSINLAIDDESIKMRWIQSIRFLE
                     SKSVVGIRFSKEILPFISNLSREFTKYSLSDIAGMSSAYAIRIYELLSQYRSIGKREIS
                     IESLRSMLELGKRYPLSADLKRWVIDTAVVQINEHSPLNVSYEQIKTGRKVTHIHFTFK
                     EKSKSIEHKSEQNNFYKLTDSQINMFGNQLSRLHEVSHLAQQGESYDDLAIKIKDMLRD
                     PIKQKQLIPHLKNLGFKA"
                     /name="repM"
     gene            5887..6504
                     /locus_tag="BEN74_RS00845"
                     /old_locus_tag="BEN74_00840"
     CDS             5887..6504
                     /locus_tag="BEN74_RS00845"
                     /old_locus_tag="BEN74_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication DNA-binding protein"
                     /protein_id="WP_026040226.1"
                     /translation="MKSLSVIELSNLYGITRQAIYKQVNKGNLSKNSDGKIDLSEAIRV
                     FGEPSRNVNSSQTTETRKLSEVHLLEQQVYMLQKQLEQAHEREQFQREELKAKNDQLHV
                     KDEQIEAIQRLLEAPKAYTTSSIDPKLDIATDTRSKSESNYDVLTTQPRETPVEVEIQT
                     QPKDDGLTTPEQVENKRIPVPEHIDQEPEKRGFWSRFFRPYD"
                     /name="plasmid replication DNA-binding protein"
     gene            6709..6984
                     /locus_tag="BEN74_RS00850"
                     /old_locus_tag="BEN74_00845"
     CDS             6709..6984
                     /locus_tag="BEN74_RS00850"
                     /old_locus_tag="BEN74_00845"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004967304.1"
                     /translation="MKNKIEDLKSTIQELIELFEEQNLEHWAEMYKRILFYIDDDLEYA
                     KYKIQSTIGGMGSMDDIVLYRNGDPLIKENNRLDYLRSKLYVFCES"
                     /name="hypothetical protein"
     gene            complement(7066..7368)
                     /locus_tag="BEN74_RS00855"
                     /old_locus_tag="BEN74_00850"
     CDS             complement(7066..7368)
                     /locus_tag="BEN74_RS00855"
                     /old_locus_tag="BEN74_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_001140620.1"
                     /translation="MAKTLQELLASRSPESQARIQKMADELLLENQLHLIREELEISQK
                     ELAETLGIKQPSLSAIENRGNDLKISTMKKYVEAMGGKLRIDVELPTGKHIGFNV"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(7361..7717)
                     /locus_tag="BEN74_RS00860"
                     /old_locus_tag="BEN74_00855"
     CDS             complement(7361..7717)
                     /locus_tag="BEN74_RS00860"
                     /old_locus_tag="BEN74_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005194067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_004967307.1"
                     /translation="MWIVITTDLFNEWLEQQDESTQEKVLAALVVLQQQGPSLGRPLVD
                     TVYDSKFTNMKELRVQHRGKPLRAFFAFDPLRQAIVLCIADKGSKKRFYKDMLDIADEQ
                     YQLHLTTLGDKSNG"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(7938..8774)
                     /locus_tag="BEN74_RS00870"
                     /old_locus_tag="BEN74_00865"
     CDS             complement(7938..8774)
                     /locus_tag="BEN74_RS00870"
                     /old_locus_tag="BEN74_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="WP_000480968.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLKP
                     IEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATEIA
                     AELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMMSNA
                     SELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDPRRIA
                     QMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQTSY"
                     /name="aminoglycoside O-phosphotransferase APH(6)-Id"
     gene            complement(8774..9577)
                     /gene="aph(3'')-Ib"
                     /locus_tag="BEN74_RS00875"
                     /old_locus_tag="BEN74_00870"
     ARG             complement(8778..9577)
                     /name="aph(3'')-Ib"
                     /gene="aph(3'')-Ib"
                     /gene_name="aminoglycoside O-phosphotransferase
                     APH(3'')-Ib"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             complement(7942..8774)
                     /name="aph(6)-Id"
                     /gene="aph(6)-Id"
                     /gene_name="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          4915..5810
                     /name="rep_cluster_1357"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.649"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(2002..3281)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.379"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gagctgaata gcgcgtattt gtagggtttt ggcatctata ccaatatgta gtttacgcca
       61 ttggcgacga tattcagctc catgtttctt gcgtttccat tcgccctcac ctagaaactt
      121 catgcctgta gagtctacga gtagatgcag tccattgcaa ctcttttcat agctaatttg
      181 aatatcaata tgcttttgtc ttctgcgaat ggttgaataa tccagagctg tccaatctaa
      241 tccgcaaagt ttaatcaagc tttggacaaa gccagtcacc atgcatacag aaagacgaaa
      301 taaggattta atcattaagc agcattggat agctgcgtcg gagtaggttt gatttcgccc
      361 ttgtttgcct tttgatggag cataccattg cgtagcagga tcaaaccaaa tggcaatatt
      421 tccgcgactc atgagtgctc ggttatatgc gggccaattg gttgtgcggt agattttgtg
      481 tgtaggcttc ttcatttgaa aattatatcg ctgaaaaagc ctttacagat aggtttgtgc
      541 aacaaagccc catcgaggtg aaaccggctg tgagcgcggg ttcaagcata tagcccgaca
      601 ggcgcgtatc cttgccgatc acgacacgat ggcggtggtc accgcgacga aagacacggc
      661 cagccgccat gccgacgcgc aaggcggttt ccgccgtcat cgcgccttcg ttggctttgc
      721 cacgaatacc gtctgtgccg aaatatttgc gcaccataag gtcgattatc ctgtcgtcgg
      781 gtcgccctca aaggggacat gcctgctgaa ccgcgaatat agagaaatat cccgaatgtg
      841 cagttaacga attcttgcgg tttctttcag cgccgccaat accgccagcc cgtcgcgcaa
      901 ggggcgcggc tcgtgtgtgc ggatgaagtc agctccacct gcggcggcgg caagctctgc
      961 agcgagtgtc gcggccccga catcccccgg accacggcct gtgagcgcgc gcagaaagga
     1021 tttgcgcgaa acagacagaa gcaccggcaa atcgaagcgc agccgcaatt catcgaaccg
     1081 cgccagcacc gagagcgagg tttcgggagc agcccccaga aaaaacccca tgccgggatc
     1141 aaggacaagg cggttgcgtt tgataccggc acccgtcagc gccgcgatgc gcgcgtcaaa
     1201 gaacgccgca atgtgatcca tgatgtcgcc agcgggtgcc tcgcgccgat ctgcctgccc
     1261 gtcttgcacc gaatgcataa cgacgagttt ggcagatgat ttcgccaatt gcggatagaa
     1321 cgcagcgtct ggaaaaccgc gaatatcatt gagataggcc acaccacgcg acaaggcata
     1381 ggcttgcgtc gcgggttgat aactgtcgag cgagacggga atgccatctg ccttgagcgc
     1441 gtccagcacc ggcgcgatac gcgcgatttc tgtgtcggac gaaacaggcg cggcgtcggg
     1501 attgctggat gccggaccga ggtcgatcac atctgccccc tcggccatca gcttacgcgc
     1561 ctgcgcaatg gctgcgtctg gcgccagata ccggcctcca tcggagaaac tgtccgaggt
     1621 tatgttgacg atgccgaaaa tgatgagcga tttattcatg ggggcttcta taataataat
     1681 aatcgagcat gagtctcata cggatgctcg ggtcgaaagg gaatccccag gcgagtaacc
     1741 tgtttgcggt gatctgaacc gtaccgggtt tgtcggagac ttttttattt aagttaggcc
     1801 acctgaccta acggattaat tttatcatag tacattgctt caaaatcaaa aggtggcaca
     1861 tagcttcgat caaaaaacga ttgacgcatg tagacaaagt agtgaggaac gtttagaacg
     1921 gctgatttca ggtgaatcta cctttgaaga agaagacgcg gtactaatga tgcaatggcg
     1981 cgccttaaat aatcgaccta aaaaccagga ctaaaatcat tatcctgatc gtgattcctt
     2041 actttttcag aagttacagc tttttccctc attttcaaag gcacacgttc aatgacttgt
     2101 cgctgtataa atgctaattc ttcatcaata ggggcgatct gaataactga accctctaag
     2161 ttttggccga gatcccttaa atatcccaac ttctcaagtc gatcgaacga ttcagtattc
     2221 aaaagatcaa tataacgctg taaatcatgc tgaatcaatg ttataggttc agtcgtttga
     2281 ggatctttca ataaagtttg cagcatattc tgctgtaaaa acaataattt ttctcgcgca
     2341 taccactgtt gtcgagtacg tggcgtttca gcttgcttgc ttagttcttc aatctctttt
     2401 cgatttttta attcagcctc ataaaacgaa cgatactgct cataacgatt ttcagcgcga
     2461 atacgttgaa tttcttcaat catatgctgt tgtttggccc acaactcagg ttcattctca
     2521 cacgcaaaac gtaccgcctt attcatcacg gggacatcct gcagctgggt tccatacttc
     2581 ttgttgtaat ctcggtgtga taaacaactc accctactcg gcacttctat ctgaatacct
     2641 cgaatctcaa cctgtttcat tggcgcatac cgcataagac tgtcattaat cagttcttca
     2701 gttttctgtc ggtacaactg cagctgtgct ttgcccatcc ccaatacatc agccgtgagc
     2761 ttctgcgcac cacctttctg cggatccttg gggttataac gcttaaaaaa ctgttcggct
     2821 gaacgatcaa tcccatccat atgccgttca ctgtacatca gatgcaaatg cggttgctca
     2881 atcccggcaa ttaaacccgc atgattatgg atggcgcagc taaatggaaa acgatagcgg
     2941 tcggcaaact cggtaataaa cgcttcagcc aactcaatcc gttgcgcttg gtttaattct
     3001 ttgggtaaag ccacgaccag actggaacag gttcggccat tactccgttc atatacatct
     3061 gcagcctgcc aaaattcagc cggttttccc tcggcaaaac tgggtaaatt cccggatttc
     3121 acaaactcaa tgtgctcaga aaccgattcc ttataacttt taaagtgtga cgttcgggtg
     3181 atgtaatgaa agcgattctt ggctgtggcg ccatctttga tttttcctga tcgtgtcttt
     3241 atcgcttttt ttgcggtgtg ttcaaggttg caatagaaca tatatcacct tcatttttag
     3301 ccggggcctc gcagagcctg cggagcacga ggaggagtgt ttctaattcg atataattgc
     3361 gtagtaatta tatcgaatta gaaacacgga aacgagctta agaattaaaa aaaatgttcc
     3421 cacatttttt ttaattcttc ccgtgccggc ctatgctaaa ctgaaaacac aaaaacctca
     3481 cccattttat gctggagaat gcgatggcgg cccgtggaag acctgcgaaa atactcactt
     3541 caaatgacct catgacatta cagcagttcc ttaaaaatct ccctttcctc aagaaaaacc
     3601 agcagcaggc tttgaccttg cttcaacaag caaatggcat cttcaatgac aaacagctca
     3661 atctactgaa agctgcagac cgtgataaaa accagttttt aaaaagacag gccctcattg
     3721 aacagatcaa gctaaaggat aaaaaccagc agccccttct ggccaacgaa acagaaattc
     3781 tggcattact gacacaggaa atggatcagg ataatttctt ccgcctggat cgtgccctgg
     3841 agtcttacca gaaaattgaa aaagcagcct tggaaaaccg gatccggctg gagaatgaac
     3901 acaagcgcga aattttgcaa aaaagtcata aagaactgac agacgcccaa aagaaacgta
     3961 atgcagaaaa tcagctcaaa tatgctttag gtggagcagt actagccgcc tggaacaaac
     4021 ttaatttatc aaccgaaaat atagatccag aaaaagttaa aaataccatc gtcaataacc
     4081 aaaatttttt cagaatggtc agtaacacca cgctttatca atatattcat ccaagaactg
     4141 aaaactactt tcgaagcaga gaattgttta tcaaagttat tgaaggatta tgtgaatatg
     4201 atgaccacgg tacagaactt tatatctttg aagcagatag tcacttaaaa ccgcaataaa
     4261 atgcgattac agccactttt aaaccaattt ttgagttagc tacgtctaaa tcaataaagt
     4321 ggctgtaatc gcaaaacaag agcatttagc ttattttgca aaaatacaaa tcaagatcca
     4381 tttcaaaact cacaatatac ctagaaggta tataccgagc gagtgtctac gagcggcata
     4441 actaaaattc gcgcttcgac tctttgaaaa actgtttttt agtatgtgaa atctaaaaga
     4501 gcgtaaatca aatgccaaaa tttgattcta gaacgagcga aagcgagtaa aaaaagcact
     4561 ataagcgaag cgaattccga attgtttgtg ctttttttaa agtcataacg agatttacta
     4621 aaaattgccc caagaatcga gcgatagcga gattcaatag agtttgagcg tagcgaaaac
     4681 atagggcaat ttttcattct ttccttttta atatatttta aatcttttaa atcttttagg
     4741 ctatctgaaa ggctttaaca gaaaggcttt cagctcctaa ctatgaggga ttgactacta
     4801 actatgaggg attgactact aactatgagg gattgactac ttaactatga gggattgact
     4861 accaaatatg agagaattat attactcata gttagtttat cgaattaaaa atgatgagta
     4921 catctaaaaa agaattagta gttaaatcaa atcaagtaat tgaggcttca tatcaactca
     4981 gctcaacaga acaacggatt gtcttagcag ctattagtaa gattagtcgg gctgaagata
     5041 ttacagatga tgaaatttat cgcgtaacta ttgatgattt aaaaaaactt ggggttcatg
     5101 aaaaaacagc ttatagagat ctaaaagatg gtgtaaaccg cttatatgac agatcaatta
     5161 atttagctat tgatgatgag tcaatcaaaa tgagatggat tcaatccata cgctttttag
     5221 aaagtaaaag cgttgtagga attcggtttt ctaaagaaat tttgccgttc atttctaatc
     5281 taagccgtga attcactaag tattcgctat ctgatattgc tggtatgagt agcgcatatg
     5341 ctattcgtat ttatgagcta ctaagccaat atcgttccat aggaaaacga gagatttcca
     5401 tcgaatctct aagaagtatg ttggagttag ggaaaagata tccattgtct gctgatttaa
     5461 agagatgggt tattgatact gctgtagttc aaatcaatga acatagccct ttaaatgtat
     5521 cttacgagca aataaagaca ggtcgaaaag tcacacacat tcacttcacg tttaaagaaa
     5581 aatcaaaaag tattgagcat aaatccgaac agaataattt ttataaattg acagattcac
     5641 aaattaatat gttcggtaat cagctttcac gcttacatga agtatctcac ctagcacaac
     5701 aaggtgaaag ttatgacgac ttagctatta aaattaaaga tatgctgcga gatccaataa
     5761 aacaaaaaca acttattcct catcttaaaa atttaggctt taaggcataa aggtcgattg
     5821 tgtatgtagc agttgactgt ttgttaatgt attttagttc acataatgga caaccaaaat
     5881 tacaagatga aatcactatc tgttattgag ctttcaaatc tctatggaat aactcgtcaa
     5941 gccatttata agcaagttaa taaaggtaat ttaagtaaaa atagtgacgg taaaattgat
     6001 ctttccgaag ctattcgagt ctttggtgaa ccttctagaa atgtaaacag ttcacagaca
     6061 accgaaacac gaaagttgtc agaagttcac ctattagaac agcaggttta catgcttcaa
     6121 aaacagcttg aacaggctca tgagcgtgag cagttccaac gtgaggaact aaaagctaag
     6181 aatgatcagt tacacgttaa agatgagcaa attgaagcaa tacaacgcct actagaagct
     6241 ccaaaagctt atacaactag ttctatcgac ccaaaattag atatagcaac agatactagg
     6301 tcaaaaagtg agtcgaacta tgatgtattg actactcaac caagagaaac tcctgtagaa
     6361 gtagagatcc aaactcagcc gaaagatgac ggattgacta ctccggagca ggtggaaaat
     6421 aagcggattc cagtaccgga gcacattgac caggaaccgg agaaaagggg cttttggagc
     6481 cgttttttta gaccctatga ttaactacga ttgtttaact gatatgaaca aagtcgtgaa
     6541 acattgatca aataataagc gtggaacgtg aaaaatcata aaaaaagccc atctaggtat
     6601 agaagggcta aaaatggaaa cctactgcac tgatttctaa agagaattat aacacatttc
     6661 gtataaggtg tattatgtta attttagaga atcttaaaaa atacttttat gaaaaataaa
     6721 attgaagatt taaaatcaac aattcaagaa ctaatagaat tgtttgaaga acagaattta
     6781 gaacattggg cagaaatgta taaaagaata ctgttctata ttgatgatga tctagaatac
     6841 gcaaaatata aaatacaaag taccattgga ggaatgggat caatggatga tatcgtttta
     6901 tatcgcaatg gtgatccact tatcaaagaa aacaatcgtt tggattactt aagaagtaaa
     6961 ttatatgttt tttgcgaaag ctaaattatt cttaatatcg ctaatttaac ataatggcga
     7021 ttatacgaat ctaaaaatgg gagtctttag ctcccatttt tattgttaaa cattgaatcc
     7081 tatatgtttt cctgttggaa gctctacatc aatacggagc ttcccgccca ttgcctcaac
     7141 atactttttc atggttgaaa tcttaagatc attgccacga ttttctattg ctgaaagcga
     7201 tggctgtttt attcccaaag tttcagcaag ttccttttga gagatttcaa gttcttcacg
     7261 aataagatga agctgatttt ctagcagtaa ttcatctgcc attttttgaa tacgggcttg
     7321 gctctctgga gagcggctag ctaacaattc ttgcagagtc ttagccatta gatttatctc
     7381 ctagggtggt gagatgaagt tggtattgtt catctgcaat atccagcatg tctttataaa
     7441 aacgcttttt actgccttta tcagcaatac atagaacaat agcctgtcgt aaaggatcaa
     7501 atgcgaagaa agctctcagt ggtttacctc gatgttgaac acgaagctct ttcatgttgg
     7561 taaatttaga gtcatagaca gtatctacta aagggcggcc taaacttggt ccttgctgct
     7621 gtagaacaac tagggcagcc agaaccttct cttgtgttga ttcgtcttgc tgttcaagcc
     7681 attcattaaa tagatctgtc gtaatgacta tccacatacc acaaccaaaa tatagattat
     7741 agtctatatt ttaggagcat tatgcttgtt ttgcaaatga gaaatgatag gcaataaaaa
     7801 accccagcat cctgccgggg tttttcatat tggggtcgtt tgcgggaagg ggcggaatcc
     7861 tacgctaagg ctttggccag cgatattctc cggtgagatt gatgtgttcc caggggatag
     7921 gagaagtcgc ttgatatcta gtatgacgtc tgtcgcacct gcttgatcgc ggccgcgata
     7981 gctagatcgc gttgctcctc ttctccatcc gcgttccaag ctgcggaaag gcacccataa
     8041 gcgtacgcct ggtcgagcag gcgacgcgga tcgacgtcca gcgcacgaga gaatgcgtcc
     8101 gccatctgtg caatgcgtct aggatcgaga caaaggtcgt ctctgtcagc cggatcgtag
     8161 aacatattgg cggcgccaaa gcccacttca ccgaccagac cgacgggatc tatcaccagc
     8221 cagccgcgac tggagaacat gatgttttca tgatgcagat cgccatgtag cccacgcagt
     8281 tccgaggcat tgctcatcat ttgatcggct ataatcgccg cgtggacgta gtcagtttga
     8341 caacctgcgt tttgatcatc gcgcgcccgc tgaaacaaag ctgcaaagcg atcccggatc
     8401 gggagaaggg cagaaggcag gggttcctca gatgcggcat acagcttcgc cattagttcc
     8461 gctgcaattt cggtcgcctg gtagtcgccg tgctcggcaa cgatgtgaga gagcattcgc
     8521 tccccggcat attcgagcaa catcagattg ttctcacgac cgagcaaccg gactgctccc
     8581 ctcccattgc gccataccag atagtcggcc ccgcgcagtt catcagcaat gtcttctata
     8641 ggtttcaatc ccttgacgat tgcaggagtc ccgtctggca atgaaacttt ccaaacgagg
     8701 ctggaaaagg tgtccgcaat gagaacaggt tgcgaaacgt gccaatgagc aggaaaaaca
     8761 ggcggcatga acatcaaccc caagtcagag ggtccaatcg cagatagaag gcaaggcgtt
     8821 cgcggtcggg ggcttcgatc cccaatacat tgaataggac agcgaaggcg cgctctgctt
     8881 catctggcgc tgcccagttc tcttcggcgt tagcaatcat gagtgccaaa tcggcatagc
     8941 gatctgctgt tccgagccgc ccaaggtcga tcagacccgt gcattgaaga gttttagggt
     9001 ccaccatgaa gttcggcatg cagggatcac catggcaaac aaccatatcg gtgcgctctt
     9061 ggtcgagccg caccggtagc tctcgttcga cacgagccaa aagatcgagc tgcggcgtac
     9121 tcttgtcctc gtccggtaag aagtcgggat tgacggcatt gcgggacacc acatcaacgg
     9181 cgcgtccgaa cattcgcgac agcctgcgct caaacggaca ttgatcaacc gataggctgt
     9241 gaacagcgcc aagttgctgc cccattgacg gccacgcttt gagcaaatcc gctccagaca
     9301 gatcagccgc cggtactccc ggaattgccg ttatcaccaa gcatgcaccc tcctgttcct
     9361 cctgccagtt gatgacctcg gggcaagcca cacctcgacc tttgagccaa atgaggcggt
     9421 cacgctctcc agcgagctca ccgcggcggg aagcaggtgc gattttcgcg aaggcatgcc
     9481 cgtcaccacg tcgaaaaaca aaatcaccag attctccgcc tctgacaggc aaccagtcag
     9541 aatgcgattc accaaaaaaa atattagttc gattcaatgg aggttccttc agttttctga
     9601 ggctttgttg cacaaagatt tgaaatgcaa agcgccccta attgatccaa atttaaggcg
     9661 taacttgggt aagtgggcga cctaattccg taaatctgtt aaggactgct acacgtgcat
     9721 ggatctcatt cacctgacta ggaaagcttc ttgccattaa tttatcgcct aataatttga
     9781 tgcaatgcat cttggtttcc accaaactgc ggcgatgata gcctgaccat tttttccata
     9841 atgtcctgcc taaatgttta actgttcgaa gtaattcatt tcgctctagc gagctactct
     9901 ttgtatcttt ccatggtttc gcattttttc taggtggaat caccgcatgc gcttgccgat
     9961 ctgcaatgac ctgacggcat tgcttggtgt cataagctcc atcggtataa acagagtcaa
    10021 tctgctcatc ttgtggaatc tgattaagta aatcaccaag cacctgtgaa tcactgacat
    10081 tattggttgt
//
