LOCUS       NZ_CP032274            10922 bp    DNA     circular CON 05-JAN-2024
DEFINITION  Acinetobacter sp. WCHAc010034 plasmid p6_010034, complete sequence.
ACCESSION   NZ_CP032274
VERSION     NZ_CP032274.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN05356835
            Assembly: GCF_001696615.3
KEYWORDS    RefSeq.
SOURCE      Acinetobacter sp. WCHAc010034
  ORGANISM  Acinetobacter sp. WCHAc010034
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter.
REFERENCE   1  (bases 1 to 10922)
  AUTHORS   Hu,Y., Long,H., Feng,Y. and Zong,Z.
  TITLE     The complete genome of Acinetobacter sp. strain WCHAc010034
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10922)
  AUTHORS   Hu,Y., Long,H., Feng,Y. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2018) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
REFERENCE   3  (bases 1 to 10922)
  AUTHORS   Hu,Y., Feng,Y. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAR-2017) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.6
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology             :: Illumina HiSeq; MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/04/2024 21:32:37
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,697
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 112
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 21 of 68
            Pseudo Genes (incomplete)         :: 53 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 16 of 68
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP032274.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10922
                     /organism="Acinetobacter sp. WCHAc010034"
                     /mol_type="genomic DNA"
                     /strain="WCHAc010034"
                     /isolation_source="hospital sewage"
                     /db_xref="taxon:1879049"
                     /plasmid="p6_010034"
                     /geo_loc_name="China"
                     /collection_date="2015-10-31"
     gene            join(10491..10922,1..366)
                     /locus_tag="BEN74_RS00740"
                     /old_locus_tag="BEN74_00735"
     CDS             join(10491..10922,1..366)
                     /locus_tag="BEN74_RS00740"
                     /old_locus_tag="BEN74_00735"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing protein"
                     /protein_id="WP_086374408.1"
                     /translation="MKRIIYEMQGLTWQARKKNQQDAILIKNKVIYEKGRYSSLDISTD
                     KNAWCVAVADGISSQPNSDKAAIAVLEIINEYYKNNTADIRLSYIQEVLCSKLKAKSVI
                     DSATTMALIRHSAEDEYNQIRIQSLGDSRVYYFSHQLQSWSILTKDDNFFNEMDISNFS
                     EESIAVLNSGQELASIYDTLTGYFCANSIHEVDEKITQIHKVEPNDVLMVCTDGVFDAI
                     THDEWPLIQINQSLKEWLKNFVEMLKPFAGDNISLVLVKATTD"
                     /name="protein phosphatase 2C domain-containing protein"
     gene            564..980
                     /locus_tag="BEN74_RS00745"
                     /old_locus_tag="BEN74_00740"
     CDS             564..980
                     /locus_tag="BEN74_RS00745"
                     /old_locus_tag="BEN74_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005198828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_068912156.1"
                     /translation="MKLFLDCEFNGFGGELISMALVDENERYFYEVLPCINPTSWVLDN
                     VIPILNKQTIDLKEFQNTLFKFLNHYETIHIVADWPEDFSLFLTSLILKQGICMITPKL
                     TMELWDSDTGLATESRIPHNALQDAFALKRKYLI"
                     /name="hypothetical protein"
     gene            1374..2027
                     /locus_tag="BEN74_RS00750"
                     /old_locus_tag="BEN74_00745"
     CDS             1374..2027
                     /locus_tag="BEN74_RS00750"
                     /old_locus_tag="BEN74_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_005086345.1"
                     /translation="MYNVHPSEKLIHAFRQKPYQGCCPTQAEFLFIGLDANYAPNIEEQ
                     KIFSKIIEYHEDAISFWQKYNVHHPFLLDGYKGDGRKYHKEFSKIRLSCKDASRISFIE
                     LLHLPTTGRNDLKSSDLDKNHLQYIHKAIFSEHTKAVFISDAVFKLMKKTSIFSWMNDA
                     KQIEGHTLKVFHEKKPLIYKHLHFSTYGKFQAQKEEEIKAIHNIILKSNISDLT"
                     /name="hypothetical protein"
     gene            complement(2052..2426)
                     /locus_tag="BEN74_RS00755"
                     /old_locus_tag="BEN74_00750"
     CDS             complement(2052..2426)
                     /locus_tag="BEN74_RS00755"
                     /old_locus_tag="BEN74_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_086322809.1"
                     /translation="MSDIQEKKDAVLKKLTKAQQKKLSLQEQIEYLKKEQEQILEQAKQ
                     KQNELKLKQQKLLSEAKAQERKNETRRKILLGAWVLNKFKNDESFKHQWADFEQFLSAE
                     SKTEENRQKDKELFNDLFKG"
                     /name="hypothetical protein"
     gene            2685..4439
                     /gene="mobQ"
                     /locus_tag="BEN74_RS00765"
                     /old_locus_tag="BEN74_00760"
     CDS             2685..4439
                     /gene="mobQ"
                     /locus_tag="BEN74_RS00765"
                     /old_locus_tag="BEN74_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002011654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_068912169.1"
                     /translation="MAIFHLNESNISRSDGRSAVACAAYRACEKLEDLTFGKTQDYTKK
                     KGLEYKNIYAPEHTNTKLLDRQTLWNTVEKKEFNANGSMKANARLAKEYICALPHELTD
                     AERIKIVDDFCRDFVNKHNVIVDACIHAPHEHNDETNNKNYHVHMMFTTRLINEKGELG
                     KKQRIFNDHGPEILKDSRATFANVVNTVLENAGLDERIDHRSYKDQGLDFLEPTHHEGH
                     EATALRRQYDEEQKRPLEERNTEIVLPRIALENDAIKAKNLDAAREYQQIIKGLDQEII
                     VPSRLEDQITQLENELQLTEAEEKELLAELVNLNLEEERLQEQQVQQIDNAYDDFIRCQ
                     DIYAEFANQFYTIQSNAADNQKQIESNLTKTKRWLAENKSDFYLHTNNLFYDSYHHTYR
                     DIKKPDFYATEKSVEQAKNENWREYATEVEQLAKEYDIENVVQRLGQCSEILENNGIER
                     PTIKPTFWQKLKREYVHSFDTLHDFNDDMKPILVEKRADDLKIEQERMQQVRQAEVDRQ
                     RKIENDRRESEFREQLRKEREQKEQRYEQERHEREHLAFLKRQELEKQQKNEPKKPENE
                     NNNDYRPW"
                     /name="mobQ"
     gene            5075..6019
                     /gene="repM"
                     /locus_tag="BEN74_RS00775"
                     /old_locus_tag="BEN74_00770"
     CDS             5075..6019
                     /gene="repM"
                     /locus_tag="BEN74_RS00775"
                     /old_locus_tag="BEN74_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798284.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepM"
                     /protein_id="WP_068912167.1"
                     /translation="MKNDLVVKDNALINASYNLEVTEQRLILLSIIRARETGQGISSDS
                     KLEIHASDYASRFDVTKEAAYNALKNAVNNLFERKFSFKEIHKDTNKEIVVKSRWVSRI
                     AYVDDLATLEVTFAPDVVPLITRLEKHFTSYQLKQVAQLTSKYAIRLYEFLIAWRDVGK
                     TPVISLSEFREKLGLEMNEYQKMVNFKSRVLEPAIKQINELTDIHVKYEQYKTGRSISG
                     LSFTFKHKKIDSIPSERDPNTVDLFTQMTDAQRHMFANKLSELPEMGRYSQGTESYPQF
                     AIRIAEMLHDPEKIKELYPYLKKVGYMPLNKRI"
                     /name="repM"
     gene            6023..6577
                     /locus_tag="BEN74_RS00780"
                     /old_locus_tag="BEN74_00775"
     CDS             6023..6577
                     /locus_tag="BEN74_RS00780"
                     /old_locus_tag="BEN74_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004282116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication DNA-binding protein"
                     /protein_id="WP_068912165.1"
                     /translation="MAKLSLSEVSNRFNVSRSTLYRAIKEGRISRSADGLFDIAEVIRC
                     FGEPIKKHEQKQEVDQSKDDADLRQLVDFMKKEIESYKDREKRYLDQIDRFQLLLGHKE
                     SEEKMSHDTPVTHSNDAPCDNNSGISETIDNNRDSEIKTSHETLVTRPNETPKHVPNTH
                     YETKKKRGLFGRVLNAVFDHD"
                     /name="plasmid replication DNA-binding protein"
     gene            6598..6741
                     /locus_tag="BEN74_RS19175"
     CDS             6598..6741
                     /locus_tag="BEN74_RS19175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004786602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_162898103.1"
                     /translation="MPKLKDIALGIIVAPLLIPIMLIASYQDKKALKKELDERQKEKAQ
                     AS"
                     /name="hypothetical protein"
     gene            complement(6925..8097)
                     /gene="blaMCA"
                     /locus_tag="BEN74_RS00785"
                     /old_locus_tag="BEN74_00780"
     CDS             complement(6925..8097)
                     /gene="blaMCA"
                     /locus_tag="BEN74_RS00785"
                     /old_locus_tag="BEN74_00780"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_057991970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCA family class C beta-lactamase"
                     /protein_id="WP_086374409.1"
                     /translation="MIFNQKYFFQASFITMVMGFGMNLHASEVSQDEKNIEKVVTQSFK
                     PLMDEYGVAGMAIGVIYKGKSYEKYYGVRFKDTNESVNGQTLFELGSLSKTFTAISGTY
                     AKNQGKISFNDQPSKYIPALKNSEINKVNLLELLTYTSGNLPLQFPDDVKTDKQILEYF
                     KNWKANNPPGAYREYSNPSIGLFGYLTAKSMNVSFSSLLEEIIFPQLNLKHTYVNVPEE
                     QKMNYAFGYDENNKPIRVNPGPLSDEAYGVKSTLPDMLKFVKSNLNVDTNSPAMKKAIL
                     DTHKGYFKVADSGMTQALGWEMFSYPTTSEILQASNSKQILLGLNPVVKELSQPKSKVF
                     HKTGSTNGFGAYILFIPEEDFGLVMLMNKKIPNVDRIKAAYNVFEILKNN"
                     /name="blaMCA"
     gene            complement(8685..8957)
                     /gene="nadS"
                     /locus_tag="BEN74_RS00790"
                     /old_locus_tag="BEN74_00785"
     CDS             complement(8685..8957)
                     /gene="nadS"
                     /locus_tag="BEN74_RS00790"
                     /old_locus_tag="BEN74_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000369784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
                     /protein_id="WP_068912163.1"
                     /translation="MDNHLFDDLVSSIKEAGAIKRKEIQASRVTKLELPDIKEVREKTG
                     LSQVEFASRLHISARTLQNWEQGRRYPTGPAVTLIRILDAHPSLI"
                     /name="nadS"
     gene            complement(8950..9270)
                     /locus_tag="BEN74_RS00795"
                     /old_locus_tag="BEN74_00790"
     CDS             complement(8950..9270)
                     /locus_tag="BEN74_RS00795"
                     /old_locus_tag="BEN74_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_068912162.1"
                     /translation="MLFIETSIFTKQIKELVSDEEYRQLQQDLLVQPDKGDVVKNGGGI
                     RKVRCAQGNKGKSGGIRVIYYWVNEDHQIFFLLAYPKSVKDTLTDKETAILRQLVKEQL
                     HG"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            9468..10001
                     /locus_tag="BEN74_RS00800"
                     /old_locus_tag="BEN74_00795"
     CDS             9468..10001
                     /locus_tag="BEN74_RS00800"
                     /old_locus_tag="BEN74_00795"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_068912160.1"
                     /translation="MIEQPRLVMNNEEVIENIKKFNSEVALYAMGDQDNSITLLVENIS
                     HYRAWYAYWDKEENKYLFAPSKYIGYQNMDAKQYAELNRSYLDGRKTEIVLANWYQTLD
                     ESSDSYEDLRTKLSDYCWNHNKNLNALFRINILKQENEKDILEKDLVDLIYKVYLGLSS
                     ENKELVKRKIMNSL"
                     /name="hypothetical protein"
     gene            10067..10429
                     /locus_tag="BEN74_RS00805"
                     /old_locus_tag="BEN74_00800"
     CDS             10067..10429
                     /locus_tag="BEN74_RS00805"
                     /old_locus_tag="BEN74_00800"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_068912158.1"
                     /translation="MNNFSSQFQGVINTHFGQKILDFLNEEKTIVMLETATYLDRPALE
                     ALVPTLEARFGDELKGIKDNSNNPENIDFDRLKQTIGHMVRVIMEKHGYVIDQNGIEIP
                     NIRQTLFLTATRYKKS"
                     /name="hypothetical protein"
     Typing          5076..6030
                     /name="rep_cluster_1154"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.507"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          2908..4436
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.235"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 agtattttga ctaaagacga taatttcttt aatgaaatgg atatttctaa ttttagtgaa
       61 gagagcattg cagtattaaa ttcagggcaa gaacttgctt caatatacga tactcttaca
      121 ggttatttct gtgcgaattc aatccatgaa gtagatgaaa agataacgca aattcataaa
      181 gtagaaccta acgatgtttt aatggtatgt actgatggcg tttttgatgc tattacgcat
      241 gatgaatggc cattaattca gattaatcaa tcactaaaag aatggcttaa aaatttcgtt
      301 gaaatgttaa aaccttttgc tggagataac attagtctag ttttagtcaa agctactaca
      361 gattaaaaaa tcaatattta tatagataat gaaatattca tttatagagc tacagcttat
      421 gcaaaatgca tttgtaatgc ccaattaaag tcagatctat gacgtatcaa tacgtagact
      481 ttatatgacg attagctctt taattcatta tttttcaatt agttttaatt aaaataattg
      541 caataaatac ggtaactgtg aacatgaaat tatttttaga ttgtgaattt aatggttttg
      601 gtggagaact gatctccatg gcacttgttg atgaaaatga aagatacttt tatgaggttt
      661 taccatgtat aaatcccact tcatgggtac ttgataatgt tatccctatt ctaaataaac
      721 aaactattga tttaaaagaa tttcaaaaca cactatttaa gtttctaaat cactatgaaa
      781 ctatacatat agttgctgac tggccagaag atttttcatt gtttctaacg tcactcatac
      841 ttaaacaagg catttgtatg attactccaa aattgacaat ggaactttgg gacagcgata
      901 ctggtttggc gacagagtca agaatccccc acaatgctct acaagatgct tttgctctaa
      961 aacgaaaata cttgatctaa aataatttta agctttttga aaaaaaaacc tgaatataaa
     1021 aaaatagttt atttatttta tgttaaatag gcgatttaag gatccctatt taacataaat
     1081 ctcttatgcg aatccatgaa acatccattg aatgccccat aaaattataa ggattaagac
     1141 aaccaaaaaa acatttctaa ttttttcatt atttaagctc ctctacaatt gacataaaat
     1201 cttttatgcg aagtcaaaat gttagaaccc atttaaagtg tctatattct caccaataaa
     1261 aatgtccact tttagaatat gcgcttttgc gattttctta tcggacggtt taatatgttg
     1321 gtgtctatat cggataaact aatattcttt gcattacgtc tactgataaa aatatgtata
     1381 acgttcatcc aagtgaaaaa ctaattcacg ctttccgcca aaaaccttat cagggatgtt
     1441 gtcctactca agcagagttt ttatttattg gcttagatgc caattacgct cccaatattg
     1501 aagaacaaaa aatattttct aaaatcattg aatatcatga ggatgctatt tcattttggc
     1561 agaaatacaa tgtacatcat ccttttttat tagatggata taaaggagat gggcgaaaat
     1621 atcataaaga attttcaaaa attagacttt cttgcaaaga tgcttctcgt atttcattta
     1681 tcgagcttct tcatcttccc acaactggta gaaatgattt gaaaagctct gatcttgata
     1741 aaaatcatct acaatacatt cataaagcca ttttttcaga acacactaaa gctgtattta
     1801 tctctgacgc tgtttttaaa ttaatgaaaa aaacatctat attttcatgg atgaatgatg
     1861 ctaaacaaat agaaggacat actcttaaag tttttcatga aaaaaaacct ttaatctata
     1921 agcatttaca tttttcaact tatggaaagt ttcaagctca aaaagaggaa gagataaaag
     1981 caattcataa tattattcta aaatcaaaca tcagtgattt aacataaaat ctcttatatg
     2041 aaatttagaa cttatccctt aaaaagatca ttaaaaagct ctttatcttt ctgtctattt
     2101 tcttctgtct tactttccgc acttaaaaac tgctcaaaat cagcccattg atgtttaaaa
     2161 gattcatcat tcttaaattt attcaaaacc caagcgccaa gcaaaatttt ccgtctagtt
     2221 tcatttttcc gttcttgggc ttttgcttcg cttaaaagtt tttgctgttt gagtttaagc
     2281 tcattctgtt tctgttttgc ctgttctaag atctgctctt gttctttttt taaatactca
     2341 atttgctctt gcagggataa ttttttctgc tgtgctttgg tgagtttctt taaaactgca
     2401 tctttctttt cttgaatatc tgacatagta ttaaaccgtg atgatcaata atcaaacatc
     2461 atcatagagt caaaaaacaa agcgagcaat atcaaacgta gcgaagcgaa gttttcccgg
     2521 agtgaaatga agggcgcact tacccacttt tatcatttca ttcaaaagtg gacgttgcga
     2581 caggggaaac ccctgtaccc catgacctcg ctttagcatg gtcagcaact ttttcaaagt
     2641 tgcaaaaatc aaaaccacgg tatgcagaaa tttaggtttt taaagtggca atttttcatt
     2701 tgaatgaaag caacatctca cgaagtgacg gacgttcagc cgttgcttgt gctgcttatc
     2761 gtgcctgtga aaaattagag gatctgactt ttggaaagac tcaggactac actaaaaaaa
     2821 agggacttga atacaaaaat atttatgcac cagaacatac caatacaaaa cttttagacc
     2881 gtcaaacact ttggaataca gtagaaaaaa aagaattcaa tgccaatggc tcaatgaaag
     2941 ccaatgcacg actcgccaaa gaatatatct gtgctttacc acatgaatta acagatgcag
     3001 aacgcataaa aatcgttgat gacttttgcc gagactttgt gaacaagcac aatgttattg
     3061 tcgatgcctg catccatgca ccacatgaac ataacgatga aaccaataat aaaaactatc
     3121 acgtacacat gatgttcacg accagactca tcaatgaaaa aggcgaatta ggaaagaaac
     3181 aaagaatctt caacgaccat ggtcccgaga tcctgaagga cagccgagca acttttgcca
     3241 atgttgtaaa cacagtattg gaaaatgccg gactagacga acgcatcgac caccggagtt
     3301 acaaggatca gggcttagac tttcttgaac ccactcatca tgaagggcat gaagctacag
     3361 cacttcgcag acaatatgac gaggaacaaa aaagaccgct tgaagaacgt aacactgaaa
     3421 ttgtgctgcc aagaattgca cttgaaaatg atgcaatcaa agccaaaaat ttagatgctg
     3481 cacgagaata ccaacagatc atcaagggcc ttgatcaaga aattattgtt ccaagtcgat
     3541 tagaagatca gattacccag ctggaaaacg aacttcagtt aaccgaagca gaagaaaaag
     3601 agcttttagc agaattagta aatctcaacc tagaagaaga acgcctgcaa gaacaacagg
     3661 ttcaacagat cgacaacgca tatgatgact tcatccggtg ccaagatatt tatgcagaat
     3721 ttgccaatca gttttacacc attcaaagca atgctgctga taatcaaaag cagattgaat
     3781 caaatctcac caagacaaaa cgatggttag cagaaaacaa aagcgatttt tacctacata
     3841 ccaataattt gttttatgac tcttaccatc atacttacag agatataaaa aaacctgatt
     3901 tttatgcgac tgaaaaatca gtagaacagg caaaaaatga gaactggaga gaatacgcaa
     3961 ccgaggtcga acaacttgct aaagaatacg atattgagaa tgtcgtccaa cgcttaggtc
     4021 aatgctcaga gatactggaa aataatggga ttgaacgacc aacgataaaa cccacgtttt
     4081 ggcaaaagct aaaacgagag tacgttcact ctttcgatac cctgcatgat tttaatgatg
     4141 atatgaaacc tatccttgtc gaaaaacggg cagatgatct caaaattgaa caagaaagaa
     4201 tgcagcaagt acgccaagcg gaagtggatc gccaacgaaa aattgaaaat gatcgcagag
     4261 aaagtgaatt tagagagcaa ttacgtaaag aacgagagca aaaagaacaa cgctatgaac
     4321 aagaaagaca cgaacgagaa catttggcat ttttaaaacg ccaagaattg gaaaaacagc
     4381 agaaaaacga gcctaaaaaa ccagaaaatg aaaataacaa cgattaccga ccttggtgaa
     4441 tgctgtttaa acgcaaaaaa atagggtttt tagcgatgtt ttgctaaacc acagtagatt
     4501 tatagttgag ttaagaaaaa actcttaaat cgcaaatgag agcatttagc gagtgtctac
     4561 gagcgaacta cagggatcta aattttttca cctgtttttc cctgattaca ggctcccctg
     4621 tgtatcaaga actcagaatc agagcatccc gaatgggtgc gaactgatta cggtggtttt
     4681 atgtggagcg aagcgagtga aaaagttcta tgagcgaagc gaactcctag ttgcttttct
     4741 tttttaaaag tcacgaaatt ttaagccaaa aattgcccct acgaactgag cgaaagcgaa
     4801 gttcaataga gtttgagcgt agcgaaaaca tagggcaatt tttcctttcc tgggctttta
     4861 attattttta aagtttttaa atacttttag acacgctgaa aacctttgtt tgcaaggctt
     4921 tcaaggtgga taagactaca atttctttgt cataagacta caatttcttt gtcataagac
     4981 tacaatttct ttgtcataag actacaattt ctttgcattt gattacatcg aatgctaatg
     5041 tagtcttaat aacacttata acctatgcag tcttatgaaa aatgacttag tagtaaaaga
     5101 taatgcctta attaatgcca gttataatct cgaagtaacc gagcaacggc ttatcctttt
     5161 atcaataata agagcaagag aaacaggcca agggatctca tccgacagta aattagaaat
     5221 ccatgcttct gattatgctt ctcgctttga tgtcaccaaa gaagctgcgt ataacgctct
     5281 aaaaaatgca gttaataatc tttttgaaag aaagttctct tttaaggaaa ttcataaaga
     5341 tacaaacaaa gaaattgtag tcaaatcccg ctgggttagc cgaattgcat atgttgatga
     5401 ccttgctaca ttagaagtta cctttgcacc agatgttgtt cctcttatta caagattgga
     5461 aaagcacttt acgagctatc aacttaaaca ggttgcgcaa ctaactagca aatatgctat
     5521 tcgtctatat gagtttctaa ttgcttggag agacgtagga aaaacacctg ttatttcttt
     5581 atctgagttc agagaaaaat taggacttga gatgaatgaa tatcaaaaaa tggttaattt
     5641 taaaagtcgt gttttagaac cagccataaa gcaaatcaat gagctgactg atatacatgt
     5701 aaaatacgaa cagtacaaaa caggaagatc tatctcaggc ttatcattca cttttaagca
     5761 taaaaaaatt gattcgatac cttcagaaag agatccaaac acggtagatc tctttaccca
     5821 aatgaccgat gcacaacgcc acatgtttgc caataaactt tcagagctac cggaaatggg
     5881 tcggtattcc cagggaactg aaagctatcc acaatttgct attcgtattg ctgagatgct
     5941 tcacgatcct gaaaaaatca aagaactata tccataccta aaaaaagtgg gatatatgcc
     6001 attaaataaa aggatttagt aaatggctaa attatcacta agtgaagtat ctaaccgctt
     6061 taacgtcagt cgttctacgc tatatagggc cataaaagaa gggcgtatat ctcgaagcgc
     6121 tgatggtctt tttgatattg cagaagtcat ccgatgcttt ggagagccga tcaaaaagca
     6181 tgaacaaaaa caagaagtgg atcagtctaa agatgatgct gatttacgtc agcttgttga
     6241 ttttatgaaa aaggaaattg agtcatacaa agatcgtgag aaacgctatt tagatcaaat
     6301 agaccgcttt caattgctat tagggcataa agagtctgaa gaaaaaatgt ctcatgatac
     6361 accagtgaca cattccaatg atgcaccatg cgacaacaat agtggaataa gtgaaactat
     6421 tgataataat agagatagtg aaattaaaac atcccatgag acattggtga cacgccctaa
     6481 tgaaacacca aaacacgtac caaatacgca ttatgagaca aagaaaaaac gtggattatt
     6541 tggccgtgtg ctaaatgctg tttttgatca tgactgagca atctagggag agaaatcatg
     6601 ccgaaactga aagacattgc cctgggcatt attgtggctc cgctgctgat cccgattatg
     6661 ctaattgcat cgtaccagga taaaaaggca ctgaagaaag agttggatga acgccaaaaa
     6721 gaaaaagctc aggcttcctg attattcacc taggcttatt aaagccgtgg aacattgctc
     6781 aataagtagg cgtggaacgt gaaaaatcat aaaaaaagcc cgaacaggga tgctcgggct
     6841 ataaactaga aactacagtg ttgaattata aggataatta tacagcattt cgtataacgt
     6901 gtattatgtt aattttagag aaagttaatt atttttcaat atttcaaaaa cattatatgc
     6961 agccttaata cgatctacat ttggtatctt tttattcatt aacataacca atccaaagtc
     7021 ttcttctgga ataaataaaa tatatgcccc gaagccatta gtagatccag ttttatgaaa
     7081 aactttagat tttggttgcg acaactcttt tacaacagga tttaatccca ataagatttg
     7141 ttttgaatta ctagcctgta gaatttcgga agtggtggga taagaaaaca tttcccatcc
     7201 gagtgcttgt gtcataccac tatcagcaac tttaaaatat cctttgtgtg tatctagtat
     7261 agcttttttc atagcagggc tatttgtatc tacattaagg ttcgacttta caaacttaag
     7321 catatcaggg agtgttgatt taactccata tgcctcatcc gacaatgggc caggattaac
     7381 tcgaattggc ttgttatttt catcataacc aaaggcatag ttcatttttt gttcttctgg
     7441 aacattgacg tatgtatgtt tcaaattaag ttgaggaaaa ataatttcct ctaacaatga
     7501 agaaaaagag acattcatcg attttgcagt taagtacccg aatagaccta tacttgggtt
     7561 tgagtattca cgataagccc caggaggatt gttagctttc caatttttga aatactctaa
     7621 tatttgctta tcagttttga catcatcggg aaattgtaat ggtaaattac cacttgtata
     7681 agttaataat tctaataaat ttaccttatt aatttctgaa ttttttagag cagggatgta
     7741 tttactaggc tgatcgttga acgatatttt gccttggttc ttagcatatg tacctgaaat
     7801 agcagtaaaa gttttactta aagaacctaa ctcaaaaagt gtctgaccat ttacgctttc
     7861 atttgtatct ttaaatcgaa caccataata tttttcataa cttttacctt tataaattac
     7921 gcctatagcc ataccagcca caccgtactc atccataagt ggcttaaagg attgtgtaac
     7981 aactttttca atatttttct catcttgact gacttcactt gcatgtaaat tcataccaaa
     8041 acccataacc attgtaataa agcttgcttg aaaaaaatat ttttgattaa aaatcattaa
     8101 gctaaatttc ttggaaaaag atggtaaata taatctcata tactaaatat caaatatgta
     8161 ttaatttttt ttaaataatt tcttatataa aaatcatcta atccaacggt tatttaacat
     8221 aatggctgtt atacgaaacg aaaaataaat agtgttatat ttcaaaaagt tatgctataa
     8281 gaaattgggg acgcaaaccc aaaaagccgg cttggaaaca agtcggcttt tttatgagca
     8341 ggtttgatac ttcttgccaa taaaattgat taatatttaa tcaaaatgaa actttagtgt
     8401 gcattatcaa ctcatacact gctcaaatta tccccaattt ctgcggataa cccttggggt
     8461 aaaatttaaa cagttttgta cgcttaggcg cacaacaatt cccgcccttt gcggctatac
     8521 gacctcacgc tgatttctta tcatggggac atagagagca ggatgctcca gataagaata
     8581 acaagatcag atacaaggaa tgtgaggtac gacaggagtt ataccaaagc acccaatacg
     8641 aaaaaccccg acaggatgtt ggggtttttt attgtctata tcttttagat caggcttgga
     8701 tgtgcatcta gaatacgaat taaagtcact gctgggccag tggggtaacg tcgcccttgt
     8761 tcccagttct gtagtgttct tgcgctaata tgcagacgtg aagcaaattc aacttggctt
     8821 aaacccgttt tttcacgcac ttctttaata tcgggtaatt caagcttagt aactcggctt
     8881 gcttggatct ctttacgttt gattgcgcct gcttctttaa tcgaagaaac cagatcatca
     8941 aataaatggt tatccatgaa gttgctcctt cactaattga cgaagaatgg ctgtttcctt
     9001 atcggtcaga gtatctttta ctgattttgg ataagccaat aagaaaaaga tttggtgatc
     9061 ttcattaacc caataataaa tcacccggat accgccactt tttcctttgt tgccttgagc
     9121 acaacgtact ttacgaatgc caccaccatt tttaaccaca tcgcccttat caggctgtac
     9181 caaaagatct tgctgtaact gacggtattc ttcatcagac acaagctctt taatttgttt
     9241 ggtaaaaata ctggtttcaa taaataacat gaattaaata cgtcaatggc gtataagaat
     9301 tttagcagtt ttaagataaa aaaacgagaa ccttaagctc tcgttttgga taacgatcat
     9361 tatcttaaat atgaatgata aaaaactatt gactcaatat ttattcaccc atatattgta
     9421 ctcaacgagt cgcttaacta attgaactaa aaacaagggt ggatattatg attgaacaac
     9481 ctcgcttggt tatgaataac gaagaagtta ttgaaaatat taaaaaattt aattcagaag
     9541 tagctcttta tgcaatggga gatcaagata actctattac attgctagta gaaaatattt
     9601 ctcattacag agcttggtat gcatattggg ataaagagga aaataaatac ttatttgcac
     9661 catcaaaata tattggttat caaaatatgg atgcaaaaca gtatgctgaa ttaaaccgtt
     9721 cttacctaga tgggcgaaag actgagattg tccttgctaa ttggtatcaa actttagacg
     9781 agagtagtga tagctatgaa gatttaagaa caaaactttc tgattattgt tggaaccata
     9841 ataaaaatct taatgcactt tttagaatca atattcttaa gcaagaaaat gagaaagata
     9901 ttttagaaaa agatcttgta gatctcatct acaaagttta cttaggacta tctagcgaga
     9961 ataaagagct cgtaaaaaga aaaattatga attcacttta atttcataat tatagatatt
    10021 taatgatttt accaaagttt tgaataaatt aaaaaagagg cccgttatga ataattttag
    10081 ttctcaattt caaggtgtta ttaatactca ctttggacaa aaaatattag attttttaaa
    10141 tgaagaaaaa actatcgtaa tgcttgaaac agcaacttac cttgatagac cggcacttga
    10201 ggctttagtt ccaaccttag aagcacgttt tggtgatgaa ctaaagggga ttaaggataa
    10261 ttctaacaat ccagagaata tagattttga ccgtttaaaa cagacaatag gtcatatggt
    10321 tagagtaatt atggaaaaac atggttacgt gatagatcaa aacggtatag aaattcctaa
    10381 tattcgtcag actctgtttt taacagctac tagatataaa aaatcttaat tttcatatag
    10441 aaacttgttg aaataataca agtttctttt tatatatcca gagagctaat atgaaaagaa
    10501 tcatttatga aatgcaaggt ttaacttggc aggcgagaaa aaaaaaccaa caagatgcca
    10561 ttttgattaa aaataaagtc atatatgaaa aaggccggta tagctcttta gatatatcga
    10621 ctgataagaa tgcgtggtgt gttgctgttg ctgatggtat aagcagtcaa ccaaattcag
    10681 acaaagctgc catagccgta ttggaaatta tcaatgaata ttataaaaat aatacggctg
    10741 atattaggtt atcttacata caagaagtac tttgttctaa attaaaagct aaatcagtga
    10801 ttgattctgc tacaacaatg gctttaattc gacattcagc tgaagatgaa tataaccaaa
    10861 taagaataca gtctcttgga gatagtagag tttactattt ctcccatcaa cttcagagtt
    10921 gg
//
