LOCUS       NZ_CP032273            11378 bp    DNA     circular CON 05-JAN-2024
DEFINITION  Acinetobacter sp. WCHAc010034 plasmid p5_010034, complete sequence.
ACCESSION   NZ_CP032273
VERSION     NZ_CP032273.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN05356835
            Assembly: GCF_001696615.3
KEYWORDS    RefSeq.
SOURCE      Acinetobacter sp. WCHAc010034
  ORGANISM  Acinetobacter sp. WCHAc010034
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter.
REFERENCE   1  (bases 1 to 11378)
  AUTHORS   Hu,Y., Long,H., Feng,Y. and Zong,Z.
  TITLE     The complete genome of Acinetobacter sp. strain WCHAc010034
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11378)
  AUTHORS   Hu,Y., Long,H., Feng,Y. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2018) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
REFERENCE   3  (bases 1 to 11378)
  AUTHORS   Hu,Y., Feng,Y. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAR-2017) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.6
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology             :: Illumina HiSeq; MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/04/2024 21:32:37
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,697
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 112
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 21 of 68
            Pseudo Genes (incomplete)         :: 53 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 16 of 68
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP032273.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..11378
                     /organism="Acinetobacter sp. WCHAc010034"
                     /mol_type="genomic DNA"
                     /strain="WCHAc010034"
                     /isolation_source="hospital sewage"
                     /db_xref="taxon:1879049"
                     /plasmid="p5_010034"
                     /geo_loc_name="China"
                     /collection_date="2015-10-31"
     gene            complement(871..1242)
                     /locus_tag="BEN74_RS00655"
                     /old_locus_tag="BEN74_00650"
     CDS             complement(871..1242)
                     /locus_tag="BEN74_RS00655"
                     /old_locus_tag="BEN74_00650"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_068912115.1"
                     /translation="MLPKIKSKAFIRLLPQIFALREQGYSYEEVTAILEKQHGLILSVK
                     QFSVYLARYKNAYTSKVEKQIETSPAPKHLQESKQDPIKSESNTNDYAFGSPQHKAIVQ
                     KETEELFRSNQNIGLRQIK"
                     /name="hypothetical protein"
     gene            1955..2536
                     /locus_tag="BEN74_RS00660"
                     /old_locus_tag="BEN74_00655"
     CDS             1955..2536
                     /locus_tag="BEN74_RS00660"
                     /old_locus_tag="BEN74_00655"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="WP_068912121.1"
                     /translation="MSIFLIRHAESEANINGKTLSHASIALSENGHIQAQNLCSTLPEI
                     DHVIVSKYLRTQQTAAPILEKYKLDFEVDEHLHEFSYLSERKCANTNLNDRKAWVNAYW
                     DKMDCQHRDADDAESFEDLYLRVQAFHDKLKLVAGHYAEKNLAVFSHGQFLQLLIMQIQ
                     QPRPLSKELMQQFRSDLVCQPIRNTEVFTY"
                     /name="histidine phosphatase family protein"
     gene            2761..2895
                     /locus_tag="BEN74_RS19785"
     CDS             2761..2895
                     /locus_tag="BEN74_RS19785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004818443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_265936562.1"
                     /translation="MTRPLKPGPKPIAKSTGKVDKRRRDNKNTPNNNDDLKPSKSSEK"
                     /name="hypothetical protein"
     gene            complement(3455..3772)
                     /locus_tag="BEN74_RS00665"
                     /old_locus_tag="BEN74_00660"
     CDS             complement(3455..3772)
                     /locus_tag="BEN74_RS00665"
                     /old_locus_tag="BEN74_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004780012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_068912112.1"
                     /translation="MKHLEFVGKSTEYIGYTHVEIINSSLRLSFNVPSTNDLKKTFKKM
                     VENNYDLLMETSDKIEFELDSEVIKKIEKITKEQGVEFEEYVQNALIKLINQYELTADQ
                     P"
                     /name="hypothetical protein"
     gene            complement(3825..4139)
                     /locus_tag="BEN74_RS00670"
                     /old_locus_tag="BEN74_00665"
     CDS             complement(3825..4139)
                     /locus_tag="BEN74_RS00670"
                     /old_locus_tag="BEN74_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004999435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_068912110.1"
                     /translation="MTRLMTEKELEAFEATRDIEAEILQGIDDMLANNAVKRTVITETD
                     VALARRKAQLTQDQFAKILGVSRRTLESWEQGVRRPSGAATSLIRLFIVDPKFVKESLA
                     "
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(4120..4443)
                     /locus_tag="BEN74_RS00675"
                     /old_locus_tag="BEN74_00670"
     CDS             complement(4120..4443)
                     /locus_tag="BEN74_RS00675"
                     /old_locus_tag="BEN74_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000288001.1"
                     /translation="MYTICETPLFTKYCLVYWTQEEYEEFKTFLALNPEAGDVEPNSGG
                     IRKIRWTSGGRGKSGGVRVIYFNRLTNGEIWLLTLYSKKQTVQLSKKTLQALVEKLNDS
                     FND"
                     /name="hypothetical protein"
     gene            4587..4892
                     /locus_tag="BEN74_RS00680"
                     /old_locus_tag="BEN74_00675"
     CDS             4587..4892
                     /locus_tag="BEN74_RS00680"
                     /old_locus_tag="BEN74_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_068912107.1"
                     /translation="MKKLKYQYRQSIQSTRHDYFWKIYPNVRSSVFKKFSTHQERSFYF
                     LHRIEYKDYPLKLRVARGTGLPHVWDDLPTYVYDVAKSWKHNSKRAHQYYKEHEKK"
                     /name="hypothetical protein"
     gene            4981..5406
                     /locus_tag="BEN74_RS00685"
                     /old_locus_tag="BEN74_00680"
     CDS             4981..5406
                     /locus_tag="BEN74_RS00685"
                     /old_locus_tag="BEN74_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016660304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_068912106.1"
                     /translation="MGSEYFIIGMDSNGAAAKEEVLEIFSPYWTEKEENYYFLDYGKEI
                     REGYIVHNECHFNIDFYEDNLAVKGVTIFKPCSDIKLEKAVFQLIHQFPMIVTYPVEPL
                     LVITANQKCVEIIKEEHPDLLPDLTIVSSFEEYNSIS"
                     /name="hypothetical protein"
     gene            complement(5534..6127)
                     /locus_tag="BEN74_RS00690"
                     /old_locus_tag="BEN74_00685"
     CDS             complement(5534..6127)
                     /locus_tag="BEN74_RS00690"
                     /old_locus_tag="BEN74_00685"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_068912104.1"
                     /translation="MIKLLINEPTKVALTETYFGGLPIRVIDQPYDWPMCAECQGHMKY
                     MGKIKTDLGLELIFICENDPGMCGDEDPDGGANQVIILSELSSLEEFTIDAENTLRETE
                     YNAVILEIEADTYDDARSNWQRGGRDILGQLHGQPNWIQDDETPKCECCQQNMRFVAQL
                     EEGPEYETAMNFAGGCEFLFDCPEGKTAKFLWQC"
                     /name="hypothetical protein"
     gene            complement(6419..7276)
                     /locus_tag="BEN74_RS00695"
                     /old_locus_tag="BEN74_00690"
     CDS             complement(6419..7276)
                     /locus_tag="BEN74_RS00695"
                     /old_locus_tag="BEN74_00690"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_068912101.1"
                     /translation="MIIAKIIRSLLTISVSLTSFSAFSASISLGTVNGIHYTADTTFVP
                     VDIDNYRQYKEVKLKGNAIKKISNKINAGDYGEGVIWIDCKNKVQISKSYLSFNSNGSL
                     KEKTIHAGETPYSFLEKNESNSPLNNLCQNSSALISHPSLVRDSTNINEYHEPNEKTDK
                     KISAMSQKEYQKNIRDMDKNMAALTSHRSRLHSGRDPENPDIITKVTKENILANTKKDL
                     ELTCKKIKLLNQKKRFILDQDFYTKDLDLVPVFESLRNNRIRVTEKSNSIRAKGGQPDI
                     CLDM"
                     /name="hypothetical protein"
     gene            complement(7392..7673)
                     /locus_tag="BEN74_RS00700"
                     /old_locus_tag="BEN74_00695"
     CDS             complement(7392..7673)
                     /locus_tag="BEN74_RS00700"
                     /old_locus_tag="BEN74_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985610.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antidote protein"
                     /protein_id="WP_068912099.1"
                     /translation="MAIKLRKWDSAEHLKTEEDMQAYLQACIEESNGDAAFIAKALGNI
                     AKAKGMAQLSKDTGLGRESLYKALSGDVNPSFDTVIKVVKALNLRLAI"
                     /name="addiction module antidote protein"
     gene            complement(7675..7977)
                     /locus_tag="BEN74_RS00705"
                     /old_locus_tag="BEN74_00700"
     CDS             complement(7675..7977)
                     /locus_tag="BEN74_RS00705"
                     /old_locus_tag="BEN74_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000286964.1"
                     /GO_function="GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /GO_process="GO:0008219 - cell death [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_068912097.1"
                     /translation="MYSIYTTEVFDEWFSKLKDQQARRRIQVRIDRVEDGNFGDTEPVG
                     EGVSELRFFFGPGYRVYYCKQGQRIVILLAGGDKSTQSKDIKLALKLAQDLEEEL"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(<8100..>8195)
                     /locus_tag="BEN74_RS19630"
                     /old_locus_tag="BEN74_00705"
                     /pseudo=""
     CDS             complement(<8100..>8195)
                     /locus_tag="BEN74_RS19630"
                     /old_locus_tag="BEN74_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016654837.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /name="N-acetyltransferase"
     gene            complement(<8200..8307)
                     /locus_tag="BEN74_RS00715"
                     /old_locus_tag="BEN74_00710"
                     /pseudo=""
     CDS             complement(<8200..8307)
                     /locus_tag="BEN74_RS00715"
                     /old_locus_tag="BEN74_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004647750.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /name="transcriptional regulator"
     gene            <8317..8471
                     /locus_tag="BEN74_RS00720"
                     /old_locus_tag="BEN74_00715"
                     /pseudo=""
     CDS             <8317..8471
                     /locus_tag="BEN74_RS00720"
                     /old_locus_tag="BEN74_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000369784.1"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(8961..9518)
                     /locus_tag="BEN74_RS00725"
                     /old_locus_tag="BEN74_00720"
     CDS             complement(8961..9518)
                     /locus_tag="BEN74_RS00725"
                     /old_locus_tag="BEN74_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005262814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_086374407.1"
                     /translation="MKKKSAFLYYLDVTAPFYYFHLVPTAIALVIVSFDFPFQGLFPTT
                     IDSSISSQHKFLNDYFAICNFFVIGLIVINYLRHPLPAKYVRQIRQHYATLNKNQQSIS
                     GWLGIVFFCCTLGLMNLIWFIINDEPLQPYKEWRKGDTLTYLKSFAHPYISTIAFSLQY
                     VLIVFITLIFMNVLNNRKYRSN"
                     /name="hypothetical protein"
     gene            complement(9729..10286)
                     /locus_tag="BEN74_RS00730"
                     /old_locus_tag="BEN74_00725"
     CDS             complement(9729..10286)
                     /locus_tag="BEN74_RS00730"
                     /old_locus_tag="BEN74_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005402002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication DNA-binding protein"
                     /protein_id="WP_228200339.1"
                     /translation="MNTTKFSVMDASKAFKKSRTTIYEAIKNGELSRAHDGLIDLSELI
                     RVYGNPIGVQSSTRTEHAQKDVQVRVQSEIENVLKDQISLLKNQLDLANQREKSLMQHI
                     EDLTHRIEFKGQLEQPKTELDERKVSTHSEMTENSRPEPEPKHDGLTTPVETENKRIPV
                     PEHVEPEQPKRGWLSRFFLPNG"
                     /name="plasmid replication DNA-binding protein"
     gene            complement(10347..11264)
                     /gene="repM"
                     /locus_tag="BEN74_RS00735"
                     /old_locus_tag="BEN74_00730"
     CDS             complement(10347..11264)
                     /gene="repM"
                     /locus_tag="BEN74_RS00735"
                     /old_locus_tag="BEN74_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005021644.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepM"
                     /protein_id="WP_068912117.1"
                     /translation="MRDLVVKDNALINASYNLDLVEQRLILLAIVEARGSGKGINANDP
                     LEVHADSYVNQFNVARQTAYQALKDACKDLFARQFSYQEINKRGNVENVLSRWVSEIRY
                     IDDEATVKLIFAPAIVPLITRLEEQFTKYELQQISNLSSAYAVRLYELLIAWRSTGQTP
                     VIELAEFRQKIGVLDDEYTRMGNFKDRVLNLAIAQVNEHTDINVKCEQHKKGRNISGFS
                     FIFKQKKVITSKTSTSLELFSKMTDAQRHLFANKLSELPDMNKYSQGTESYTQFAVRIA
                     EMLQDKERFEELLPYLRKVGFNTK"
                     /name="repM"
     Typing          complement(10577..11264)
                     /name="rep_cluster_2314"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.017"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 taaacgtagt catattggta ggtaaacgta gtcatattgg taggtaaacg tagtcatatt
       61 ggtaggtaaa cgtagtcata tagatgctgt acccctttaa aacaaaggct ttgaggtagt
      121 ctaaaagcat ttaaaaactt aaaaataatt aaaagcctaa agaatgaaaa attgccctat
      181 gttttcgcta cgctcaaact ctattgaact tcgctttcgc tcagttcgaa ggggcaattt
      241 ttagtaaata ccggatgtaa ctgaaatatt aaaaagcaaa gtcaaaagca agaatggatt
      301 cgctacgctc ataaagcttt ttttctcgct acgctcggtc tagattcaat tttctgcatt
      361 tgatttactc ttatttaggc atcacagcct aaaaagctgt ttttcagaaa gtcgaggcgc
      421 gaattttagt ggttcgctcg tagacactcg ctctgtaaca tattgatcta tttaataatt
      481 aagtatcgga aattgtccac acatcatgaa taattgaaag ccaaatttgt ataattaatc
      541 attaaattgc tgttttaaag tggtcaaaag tgcagttttc agacaaaaag taatcaaatt
      601 ttatgaaaaa tgatctaaaa attgacgcaa tatgtgatgt ccataaaaca tcggaaaatg
      661 tccatatata gtcttcaaac cctttctatt aaagggtttg aagactttta taaaatgaac
      721 cctattaatt ctaataaaaa ttaaagaaat agataacatg cgtgtattta agcctgtttt
      781 tcgatttaag cttgtttatg tgtttggctt ggagtctccc tgtatttttc taaaagaagg
      841 ccttatgcgc gatttggtat cgttttacag ctattttatt tgtcttaagc caatattttg
      901 attggatcta aataactcct ctgtttcctt ttggactatt gccttgtgtt gaggtgatcc
      961 aaaagcataa tcgttcgtat tgctttcaga tttgatggga tcttgtttac tttcttgaag
     1021 gtgctttgga gctggagaag tctcgatctg tttttctact tttgaagtat atgcattttt
     1081 ataccgtgct agataaacag aaaattgttt aacgctcagg atgagtccat gttgtttttc
     1141 taaaattgca gtcacttctt catatgaata gccctgttct ctgagtgcaa aaatctgagg
     1201 tagaagtcgg ataaaggctt tggatttgat ttttggcagc acgtttttag tttccagttc
     1261 gattttctat tttgatagta aattgttagt attttaattg caaattaata tattttcagt
     1321 agtaaattcc tgattttgat acccaattga taactttttg atagtaaatc agtagtaagt
     1381 tgatatatac gcccttttgg ctgtttttct ctctaaattc tgctcaaaat ttaagaggga
     1441 tttttgacaa tttttaggta tacaccatac gcacctagtt aggatgcaaa aaaacgaggt
     1501 ttttttcttt aaaaacagga ctatatcatg ttcgaaagag agttttgaaa cgtcaccaaa
     1561 tgtcacaaaa tagatgttaa cattctgttt tatattgaaa aatttgtcac agcattgtca
     1621 caacgccatt tctatatgaa gcataaaata gaatcctcga attttaaaat tttgaggatc
     1681 ggcaatgttt gcaaatgtgt acattcgata gataagtgaa tgtaataaaa aacgtttttt
     1741 gaaaacattt tgaaaacatt gctgtctagt aaagtgcgct aaaaagtagc tgattttttg
     1801 cggtactttg tgctgtttct tgcattattt tactgatgcc ttttggcaca aagtgacccg
     1861 actttagatt caggctaaag ccaaaatcta aagcatcggg tgactctatg agcatctatc
     1921 ggctatgctc tatgcttcaa tgaaagggaa aaatatgagt atttttttga tccgccatgc
     1981 tgaaagtgaa gccaatatca atggtaaaac gctgtcgcat gcttcaatcg ctttaagcga
     2041 gaatggccat attcaggctc aaaatttatg ctctacgctg cctgaaattg atcatgtgat
     2101 agtgtccaaa tatttgcgga cgcaacaaac cgcagcaccg atcctagaaa aatataagct
     2161 tgattttgaa gttgatgagc atttgcatga gtttagctat ctgtctgagc gtaagtgtgc
     2221 caacaccaat ttgaatgacc gtaaagcatg ggtgaatgcg tattgggata aaatggattg
     2281 ccagcatcgg gatgctgatg atgctgagtc tttcgaggat ctctatctgc gggtgcaggc
     2341 gtttcatgac aagttaaagc tcgtggccgg gcattatgcg gaaaagaatt tggctgtatt
     2401 tagtcacgga cagtttttac agcttttgat tatgcagatc cagcaaccaa gaccgcttag
     2461 caaggagttg atgcagcagt ttagatctga cttggtttgt cagccgatta gaaatactga
     2521 ggttttcact tattgagctt aatttcgtat aagaaatttt atgtaaagta gatagtcgaa
     2581 aggcccatga atcgggcctt tcgtataacg cgtattatgt taattttaga aaatcttaat
     2641 cttgtcactt attcctgaac ataaaaaata atagcctttt atgaaattta tctgtctgtt
     2701 gcaattttaa taatttcatt ttatattgtt aatcaatttt acttatatag gatttttaaa
     2761 atgactagac ctcttaaacc aggaccgaaa ccaattgcta aatctactgg taaggttgat
     2821 aaaagaagac gtgataataa gaatactcca aataataatg atgatttgaa acctagtaaa
     2881 tcatctgaaa aatagctgga ggcagacgtg aaaataatgc tgtctataat tcagtagaat
     2941 cacgacaatc ctgagcatta agggggatga ttaaatttaa tcatttctct attatttaac
     3001 ataatgggcg ttatacgaag tacacttgta tattaattta aatatcaaat acttaaaaat
     3061 ttcaccccag ccaaaaaatg tacaaattgc cgattttgaa acaaatcggc tttttattga
     3121 acgaatttgt cactttcagt caataaaatc gatcaaaaaa acgctcaaat tcaacttttt
     3181 ccctgataac tcaatacatc cagtcccgaa actatccgca atttctgcgg ataaaccttg
     3241 gggtaagttt tgggtaattt ttgtacgctc aggctgacat gtgttagggg aaattacgtc
     3301 tatatcgccc cctaattttt ttcatatgat ggaattatgg agtacaggat actccagcac
     3361 ataaaacaat aatgataagt gcaacagtta ctaaaactaa tcaaccacat acgaaaaagc
     3421 ccgacaaaaa tgtcgggctt ttttattgcc taatttaagg ttgatcggca gttaactcat
     3481 attggttaat aagcttaatc aaggcatttt gcacatattc ttcaaactca acaccttgtt
     3541 ctttggttat tttttcaatt tttttaataa cttcactatc taattcaaat tctattttat
     3601 cggatgtctc catcagcaaa tcatagttat tttcgaccat ctttttaaaa gtttttttta
     3661 gatcatttgt tgatgggaca ttgaaagata agcgtagtga actattgata atttcaacgt
     3721 gggtatagcc gatatattcg gtactttttc ctacaaattc taaatgtttc ataaaagacc
     3781 ttaaacaaga tagtgaaata gctctctagt gagagctata taaattaagc cagactttct
     3841 tttacaaatt ttggatcaac aataaataat cgaataagtg atgttgccgc ccctgatggc
     3901 cgtcttacac cttgttccca tgattcaagg gtacgtcttg aaacaccaag aatcttggca
     3961 aattggtctt gggtcagttg agcctttcga cgagctaatg cgacatctgt ttcagtgatt
     4021 actgttcttt ttacagcatt attagcaagc atatcatcta tgccttgtaa gatttcagct
     4081 tcaatatctc ttgttgcttc aaaggcttcc agttcttttt cagtcattaa acgagtcatt
     4141 taatttctcc accaatgctt gaagggtttt cttactaagc tgcacagttt gctttttact
     4201 gtacagggtt aataaccaaa tctctccatt tgttaaacga ttaaagtaga tgactctgac
     4261 accaccactt ttacctctac caccactcgt ccatctaatc tttcgaatac cgcctgaatt
     4321 aggttctaca tccccagctt ctggatttaa tgctaagaat gttttgaatt cctcatattc
     4381 ttcctgagtc caataaacaa gacagtattt agtaaataaa ggggtttcac atattgtata
     4441 cattgcttgt attcatacta ccttgtagta gttaatttta tactactttg tggtatcttt
     4501 aaatatcaat ttttaattta tagaactaaa gacgatgtat tactttgcag aggatccctt
     4561 ctaggtaatg ttacctagga ggaacaatga aaaaactaaa atatcagtat aggcaatcaa
     4621 tacagagtac tcgccacgac tacttttgga aaatttatcc caatgtcaga agctctgtat
     4681 ttaagaaatt tagcactcac caggagcgta gcttttattt tttgcatcga attgaataca
     4741 aagattatcc gcttaaactt cgtgtcgcac gtggtacggg attacctcat gtttgggatg
     4801 accttccaac ctatgtttat gatgttgcta aaagttggaa gcataactct aagagagcac
     4861 atcagtacta caaagaacac gagaaaaaat aaaaaatggg ggcaatcagc tccccatttt
     4921 tgacttcgca taagagattt tatgttaaat attacacaaa ctgaataatt agagaaaaca
     4981 atgggaagcg aatattttat tatcggaatg gacagtaatg gtgcagcagc taaagaagaa
     5041 gttttagaaa tttttagccc ttattggact gaaaaagaag aaaattatta ctttttagac
     5101 tatggaaaag aaatccgtga gggatatata gtccataatg aatgtcactt taatatagac
     5161 ttttatgaag ataatttagc tgtaaaaggt gtaacaatat ttaagccatg tagtgatatt
     5221 aaattggaaa aagctgtttt ccagttaata caccaatttc caatgattgt gacttatcca
     5281 gtggaaccat tactggtaat tactgcaaat cagaaatgtg ttgaaattat caaagaagaa
     5341 catcctgatt tattacctga tttaacgata gtgtcatcgt ttgaagaata taacagtatt
     5401 agttaaatgg gagcttagct cccaattttg atttcgataa agtttattat gttggctctc
     5461 accaatatat caaaagaatt taaaaatatt tgaggattac atcaatttat tcttaatatg
     5521 acagaactaa aaatcagcat tgccataaaa acttagcagt cttaccttca gggcaatcaa
     5581 ataagaattc acaacctccg gcaaaattca tggcggtttc atactcagga ccttcctcta
     5641 attgcgcaac aaagcgcata ttctgctgac aacactcaca ttttggtgtt tcatcatcct
     5701 gaatccaatt aggctgacca tgtaattgcc ctaatatatc tcgtccacca cgttgccaat
     5761 tcgagcgagc gtcatcatac gtatctgctt caatctctaa tattacagcg ttatattcag
     5821 tttctcgcaa tgtattctca gcatctattg taaactcttc caatgaagat agttctgaaa
     5881 ggatgatcac ctgatttgca ccaccatctg gatcttcatc cccacacata cccggatcat
     5941 tttcacaaat aaaaattaat tctaagccta aatctgtttt aatttttccc atatatttca
     6001 tatgcccttg gcattcagca cacatcggcc aatcataggg ctgatcaatt acacgtatag
     6061 gtaatccacc aaaataagtt tcggtaagtg caactttagt tggctcatta attagtaatt
     6121 taatcatgaa aaaataaaaa ccttattcgt aaataacgta acaatctaac taaaaaatga
     6181 atttaacaaa aattattttt ttaattgcaa tgtttcaaaa agtctcataa taaatattta
     6241 acacaatacc aaagggtttc tttagggttc tgtcgtatta agaattaaat caatttaaca
     6301 taatggccct aagattcgat tttattcccc attgcttgat gacatggctt tgattcagta
     6361 tttcaaggaa acagtaggag cctgacgcac tcctacattg acgctttaac ttagaaaatt
     6421 acatatctag gcatatatct ggctgtccac cctttgccct gatggagtta cttttttctg
     6481 taactcttat tctattattt cttaggctct cgaaaacggg tactaggtcg agatctttag
     6541 tgtaaaagtc ttggtcaagg ataaatctct ttttttggtt taaaagctta attttcttgc
     6601 atgtcaattc caaatctttt tttgtatttg cgagaatatt ttctttcgta acttttgtaa
     6661 taatatctgg attttctggg tcacgtcccg aatgcaaacg agaacgatga gatgtgagtg
     6721 cagccatatt cttatccata tctcggatat ttttttgata ttctttttgg ctcattgctg
     6781 aaattttctt atctgttttt tcattgggtt catgatattc attaatgttt gttgaatctc
     6841 ttactaggga tggatgtgag attaaagccg aactattttg acataaatta tttagagggc
     6901 tattactttc atttttttca agaaaactat aaggtgtttc acctgcatga attgtttttt
     6961 ctttgagaga accattcgaa ttaaaagata aataagattt gctaatttgt actttatttt
     7021 tacaatctat ccaaataact ccttcaccat agtcaccagc attaatttta ttacttattt
     7081 ttttgatagc attaccttta agtttaactt ctttatattg ccgatagtta tctatatcta
     7141 cagggacaaa tgttgtatct gccgtgtagt gaataccatt tactgttcct agtgagattg
     7201 atgcactgaa agcactaaat gatgttaaag aaactgatat ggtaagtaac gaccttatga
     7261 ttttagcaat tatcaaaatt ttaccccttt aaaactctat aaacagtagc tatgccacac
     7321 ccagaaattg gacaggtcta agattgaagc caaaaatggg agccttaagc tcccattttt
     7381 gctaactttt tttaaattgc taagcgtaaa ttcagtgctt ttacgacttt aataacagta
     7441 tcaaagcttg ggtttacatc accagaaaga gctttataaa gactttctct gcctaaacct
     7501 gtgtcttttg ataattgagc cattccttta gctttagcga tattgcctaa agcttttgca
     7561 atgaatgctg catcaccatt agattcttca atacatgctt gtagataagc ttgcatatct
     7621 tcttctgttt taaggtgttc agcactgtcc catttgcgaa gtttaatagc cattttacag
     7681 ctcctcctct aaatcttgtg ctaatttcag ggcaagtttt atatctttac tttgagtaga
     7741 tttgtctcca ccagctaaga gaataacgat tctttgccct tgcttgcagt aataaactcg
     7801 atagccaggt ccaaagaaga aacgtaattc tgagacacct tcaccgactg gttctgtatc
     7861 tccaaagttt ccatcttcta cccgatcaat tcgaacttgt atgcgtcttc ttgcctgttg
     7921 gtcttttaac ttgctaaacc attcatcaaa gacttcagtg gtatatattg agtacataag
     7981 tagaatgtat cctacaggat acaaataagc aagatattga ctttaatttt gagttctaac
     8041 atcattggcg tcagttttcg aatgtagttt atttttgcaa ttccagttct aaaacttaca
     8101 ccttgtaaac catcgaaatc tttaaattct tttgatataa aattattttt aatcaaaatc
     8161 ttacgagaag ccatattaat agggtcaata attgcagttc tctttcgcaa tttgggtcat
     8221 tcctcttgct ttggcaatta caccaagtgc atgcgctaat tcagcaggat cattttcttc
     8281 aagaaccata ttgagataca tcacaatatc ttcttcgtac gtgagaaaac gggcttaacc
     8341 caagcagaat tcgctgcacg tttgcatatt agcgcgagac cctacaaaac tgggaacaag
     8401 gccgtcgcta tccaactggt cctgcagcga ctctaattcg cattctggat gcccatccaa
     8461 ctctgatcta aatattttag ataataaaaa accccggcat cctgccgggg tttttcgtat
     8521 tggttcgctc ggtataactc ctgtcgtacc ttacagtcct tgtgcgtatc gttgttctta
     8581 ttattgtctg gaacttcctg ttctccatgt ccccatgata ggaaattgca gccaggccgc
     8641 ataggcgcaa agggcaggaa ttgctgtgag catatgcgta caaggccggc taaaaactgt
     8701 cctcttttta tccgcagaaa ttgcggatag tttccgaagt gcaggcattg agttatctgg
     8761 gaaaagtgga aatatgagcg ttttttgatc gattttattg gctgaaagtg acaaatctgt
     8821 tcaataaaaa gccgacttgt ttcaaaatcg gctttttggg cattttttga ttggtgtgaa
     8881 ttttttaagt atctgatatt taaattaata tacaagtgta cttcgtataa cgcccattat
     8941 gttaaatgga aataattatt ttaattcgac ctatatttac gattattcaa tacgttcata
     9001 aatattagtg tgatgaatac aatgaggaca tattgcaaac taaaagcaat agtagaaata
     9061 taaggatgtg caaaactttt taagtaagta agagtatcgc ctttacgcca ttctttgtat
     9121 ggttgaagag gttcatcatt aataataaac caaattagat tcatcaggcc taaagtacaa
     9181 cagaaaaata caatgccaag ccagccactg atagactgct ggttcttatt taatgttgca
     9241 taatgctgac gtatttgtct tacatatttg gcgggtagtg ggtgtcttaa ataattaata
     9301 acaataaggc caatcacgaa aaaattacat attgcaaaat agtcatttag aaacttatgt
     9361 tggcttgaaa tcgaagagtc aattgtggtc gggaataagc cctgaaatgg gaaatcaaaa
     9421 ctgactatga ctaatgcgat tgcagtgggt acaagatgaa aataataaaa aggtgctgta
     9481 acatctaaat aatataaaaa agctgatttt tttttcatag tttttggtga tttcgtttaa
     9541 gtttgtctaa aattaacata atacgcgtta tacgaaatgc ttgaaatttt cctataagca
     9601 tcaatatctt agtcaaagcc gttctagggt tcagcgcact ggaacggctt tttcatgatt
     9661 attcacgttc cacgcctatt ctttgatcaa agttccacaa ctttgattag tacatctaaa
     9721 cagatcagtt agccattcgg caagaaaaac cggcttaacc accctctttt cggttgctcc
     9781 ggttcaacgt gctccggcac tggaatgcgc ttattttctg tttcgaccgg agtagtcaat
     9841 ccgtcatgtt tcggctctgg ttctggtcga gaattttctg tcatttcaga atgagtagaa
     9901 actttacgtt catctagctc tgtctttggt tgttcaagct gacctttaaa ctcaattctg
     9961 tgggtcaaat cttctatatg ctgcatcaaa gacttttctc tttggttggc tagatctaac
    10021 tgatttttga gtagagaaat ttgatctttc aatacatttt caatctcaga ttgaacacgt
    10081 acttgtacat ccttctgggc gtgttcagta cgtgtactgg actgaacacc gataggatta
    10141 ccatagaccc tgataagttc ggataaatct ataagcccat catgagccct tgataattca
    10201 ccatttttta tagcttcata tattgttgtt cgtgactttt taaaagcttt acttgcgtcc
    10261 atgacgctga attttgttgt gttcaatgta atccctcagt gcatgtacaa ggatgtttag
    10321 atgaacatcc tacctgaaca tgaagtttat tttgtgttaa atcctacctt tctaagatat
    10381 ggtagaagtt cttcaaatct ctctttgtct tgtagcattt cagcaattcg aactgcaaat
    10441 tgtgtatagc tttctgtacc ttgagaatat ttattcatgt ctggaagttc agaaagttta
    10501 ttagcaaaaa ggtgacgttg tgcatcggtc atttttgaga aaagttcaag tgaagttgaa
    10561 gttttagagg taatcacttt cttttgctta aatatgaatg agaatcctga aatgttacgg
    10621 ccttttttat gttgctcaca tttaacgtta atgtctgtgt gctcattaac ttgggcaata
    10681 gctagattta acactcgatc cttgaagttt cccattctag tgtattcatc atcgagaaca
    10741 cctatctttt gcctgaactc tgctagttca ataacaggtg tttgacctgt actacgccaa
    10801 gcaatcaata attcgtataa acgtacagca taagcactac taagattgct aatttgttgt
    10861 agctcatact ttgtaaattg ttcttctaaa cgtgtaataa ggggcacgat agctggtgca
    10921 aatatcaact ttactgttgc ttcatcatcg atgtatctaa tttcactgac ccaacgactt
    10981 agaacattct caacattccc tcttttgttg atttcttgat agctaaattg tcgtgcgaat
    11041 aagtctttac acgcatcttt aagtgcttga taggctgtct gtcgtgcaac attaaactgg
    11101 tttacataac tatctgcatg aacctctaaa ggatcatttg cattgatacc tttgccacta
    11161 cctcttgctt cgacaatagc taataggatt aaacgctgtt ctactaaatc caagttataa
    11221 ctagcattta ttaatgcatt atctttaaca actaaatccc gcatcttatt taactacatt
    11281 attatttatg aatataagtc tatattaata attatgtaga cttatgcaag gtaaacgtag
    11341 ttaaatctac aaaaaatatt atgcagacta ttggtagg
//
