LOCUS       NZ_CP032272            12457 bp    DNA     circular CON 05-JAN-2024
DEFINITION  Acinetobacter sp. WCHAc010034 plasmid p4_010034, complete sequence.
ACCESSION   NZ_CP032272
VERSION     NZ_CP032272.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN05356835
            Assembly: GCF_001696615.3
KEYWORDS    RefSeq.
SOURCE      Acinetobacter sp. WCHAc010034
  ORGANISM  Acinetobacter sp. WCHAc010034
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter.
REFERENCE   1  (bases 1 to 12457)
  AUTHORS   Hu,Y., Long,H., Feng,Y. and Zong,Z.
  TITLE     The complete genome of Acinetobacter sp. strain WCHAc010034
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12457)
  AUTHORS   Hu,Y., Long,H., Feng,Y. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2018) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
REFERENCE   3  (bases 1 to 12457)
  AUTHORS   Hu,Y., Feng,Y. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAR-2017) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.6
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology             :: Illumina HiSeq; MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/04/2024 21:32:37
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,697
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 112
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 21 of 68
            Pseudo Genes (incomplete)         :: 53 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 16 of 68
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP032272.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..12457
                     /organism="Acinetobacter sp. WCHAc010034"
                     /mol_type="genomic DNA"
                     /strain="WCHAc010034"
                     /isolation_source="hospital sewage"
                     /db_xref="taxon:1879049"
                     /plasmid="p4_010034"
                     /geo_loc_name="China"
                     /collection_date="2015-10-31"
     gene            join(12356..12457,1..201)
                     /locus_tag="BEN74_RS00585"
                     /old_locus_tag="BEN74_00580"
     CDS             join(12356..12457,1..201)
                     /locus_tag="BEN74_RS00585"
                     /old_locus_tag="BEN74_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_018680087.1"
                     /translation="MSVETLEQKIAKQEERLRQLKAQKQAIAAREKKKVSDQQRKDDTR
                     RKILLGAWALNKLKNDESFKQQLADFEQFLNTENKTEENKQKDKDLFNGFIWDKT"
                     /name="hypothetical protein"
     gene            complement(262..552)
                     /locus_tag="BEN74_RS00590"
                     /old_locus_tag="BEN74_00585"
     CDS             complement(262..552)
                     /locus_tag="BEN74_RS00590"
                     /old_locus_tag="BEN74_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004647750.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antidote protein"
                     /protein_id="WP_004787581.1"
                     /translation="MVKIADLPSFDMAEALKSEEDIVMYLNMVLEENDPAELAHALGVI
                     AKARGMTQIAKEAGIGREALYKALRQDSAPRFDTINRVVNALGLKLTVQNA"
                     /name="addiction module antidote protein"
     gene            complement(556..852)
                     /locus_tag="BEN74_RS00595"
                     /old_locus_tag="BEN74_00590"
     CDS             complement(556..852)
                     /locus_tag="BEN74_RS00595"
                     /old_locus_tag="BEN74_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004647749.1"
                     /GO_function="GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /GO_process="GO:0008219 - cell death [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_004787583.1"
                     /translation="MTEIKRLPEFDEWLDSIKDSMTRLKLNRRLDKVQRGNWGDVKHLQ
                     EGVWEMREFFGPGWRMYYIKHGDVIIVMLGGGDKSTQQKDIERAVDLSKSLED"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(1176..1817)
                     /locus_tag="BEN74_RS00600"
                     /old_locus_tag="BEN74_00595"
     CDS             complement(1176..1817)
                     /locus_tag="BEN74_RS00600"
                     /old_locus_tag="BEN74_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016927662.1"
                     /GO_process="GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="WP_004787586.1"
                     /translation="MSKKDIAPQRLTREIVLRTALDMLNEEGIDSITTRKLAQRLGIKS
                     PTLYWHFKNKSLLMEAMAETIINEHHLVSLPIDGMTWQDWLLANSVSFRRALLAYRDGA
                     RLHARTSPSQGHFNTIEAQVALLSHAGFSPVEAVALLMTLGRFIVGWVLEEQQEEIRSD
                     PPFEADPTIYPLMLQGVNTLQNMNADDIFENGIRMVIIGAERQLDIKMQT"
                     /name="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
     ARG             1950..3070
                     /name="tet(39)"
                     /gene="tet(39)"
                     /gene_name="major facilitator superfamily (MFS) antibiotic
                     efflux pump"
                     /tool="rgi"
                     /drug_class="tetracycline antibiotic"
                     /identity="100.0"
                     /coverage="94.43"
     ARG             1884..3067
                     /name="tet(39)"
                     /gene="tet(39)"
                     /gene_name="tetracycline efflux MFS transporter Tet(39)"
                     /tool="amrfinderplus"
                     /drug_class="TETRACYCLINE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            complement(3295..3582)
                     /locus_tag="BEN74_RS00610"
                     /old_locus_tag="BEN74_00605"
     CDS             complement(3295..3582)
                     /locus_tag="BEN74_RS00610"
                     /old_locus_tag="BEN74_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004728627.1"
                     /translation="MKTRAELDAMSHQELKDYEQSLLALWTPRMAIESDIERLSTNRNE
                     LLEIFNQLKNPDAPENERLKNSILSLKYKIEDLEDKLDDLIQDNRLNRAD"
                     /name="hypothetical protein"
     gene            complement(3579..4529)
                     /locus_tag="BEN74_RS00615"
                     /old_locus_tag="BEN74_00610"
     CDS             complement(3579..4529)
                     /locus_tag="BEN74_RS00615"
                     /old_locus_tag="BEN74_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001031297.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_004728629.1"
                     /translation="MNKENSYDKSYPVTTMAIQNKVTECFKSMSVDEKRILIMASPIAR
                     NIDASEQDQILISAQQFADDCGIKVNSAYKQIENASKKLVDRSFSYVNDRGKKVYSNWV
                     IDATYEDAGISLRFTSIVLVMLKILDKYNPYTRYKKDVVLKLKKDYSIDFYHLAKKNQA
                     KNGFELTLDEMFTEFGLPESYRDLRNLKRRVLKSSLDEINEFTDVTVDYSPVKKGRSVV
                     GFKFTVKEKSKPKLIAPERDPKTIDIFCNLSDAQINKYSAILSKLSELSDLSNFQDYPS
                     FALWISGILRDPKSVREETAKRIFKALHSKTDFKP"
                     /name="replication initiation protein"
     gene            complement(5212..6864)
                     /gene="mobQ"
                     /locus_tag="BEN74_RS00620"
                     /old_locus_tag="BEN74_00615"
     CDS             complement(5212..6864)
                     /gene="mobQ"
                     /locus_tag="BEN74_RS00620"
                     /old_locus_tag="BEN74_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005199240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_068913314.1"
                     /translation="MAIYHFSVKTISRGNGRSAVACAAYRSSEKLVCDFYGKEQDYTRK
                     TGVEFTKIYAPENTNTELLKRQTLWNEVEKVERRKDALLAREFEIAFPSELNAEQRENM
                     LNELCRNLVKKYGVIVDAAIHAPHTDSGSDERNYHAHIMFTTRSINEQGGFSAKKYRDF
                     SRDNGTETVSHWRESFAELCNHHLEQNGFDERVDHRSYEDQESDLEATQHEGPQATYLR
                     RRGIFTEISLKNDEIKLRNLKIKKVIALDENIKVSEDLVQKVRSELQFQYSQAEYSEKT
                     SQKLYEFQNEELSKLTTKLDTMSSAISELRKKEPLFFKTKWINQINDLIDQHNTQLDIQ
                     KHISGLSEIEKKERYSDHLNKWTEENQLLQPKKSFAKLDEPNITVKQRKLNDQISNIDH
                     QISEISRRETEYQEYVRDQRLEIINSYIFEEDNYGNKYFSPQNGEKQKRLYAEEMEDLK
                     IQELHAAFTKKIETEAQQEFSQKRAIQLENDRKDRESREQQLRKEREQQEQRYAQEREQ
                     QEHLAFVRRQEQEKQQRNEPKKPENDNNHDYTP"
                     /name="mobQ"
     gene            7076..7378
                     /locus_tag="BEN74_RS00625"
                     /old_locus_tag="BEN74_00620"
     CDS             7076..7378
                     /locus_tag="BEN74_RS00625"
                     /old_locus_tag="BEN74_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_046128218.1"
                     /translation="MSVETLEQKIAKQEERLRQLKAQKQAIAAREKKKNSDRQRKDDTR
                     RKILLGAWVLNKLKNDESFKGQLTDFEKFLSTESKTEENRQKDKDLFNGFIWDKT"
                     /name="hypothetical protein"
     gene            complement(7396..7953)
                     /locus_tag="BEN74_RS00630"
                     /old_locus_tag="BEN74_00625"
     CDS             complement(7396..7953)
                     /locus_tag="BEN74_RS00630"
                     /old_locus_tag="BEN74_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005146114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="WP_016165572.1"
                     /translation="MKEIRQQKKQPEVIRKRILEQATILVSQKGISGVSIQNIATAVGI
                     TKGGVFHHFPNKKNLIEEMFNQIILDLDQTVEYLIKRDQTEYGKFTRAYIHSSFIHQID
                     GVVSAWSALSMTMITEPTFNKIWMEWLKNRLSQHAETDSHPDLELLRLAADGLWLQSIT
                     GVIDDEQCSKLEDDLLNRTYLT"
                     /name="TetR/AcrR family transcriptional regulator"
     gene            complement(7954..8298)
                     /locus_tag="BEN74_RS00635"
                     /old_locus_tag="BEN74_00630"
     CDS             complement(7954..8298)
                     /locus_tag="BEN74_RS00635"
                     /old_locus_tag="BEN74_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005146112.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA];
                     GO:0005886 - plasma membrane [Evidence IEA]"
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family transporter"
                     /protein_id="WP_005199344.1"
                     /translation="MNSVLLAYVLLGFSIVSEVIGSSFLVKSEGFTKLFPSVMVFIFFS
                     TAFYLLSQVIKTIPLGIAYAIWAGVGIVLTAVVSYFVFKQTLDNPALIGIGFIVSGVII
                     INLFSQSAGH"
                     /name="SMR family transporter"
     gene            complement(8556..8843)
                     /locus_tag="BEN74_RS00640"
                     /old_locus_tag="BEN74_00635"
     CDS             complement(8556..8843)
                     /locus_tag="BEN74_RS00640"
                     /old_locus_tag="BEN74_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004870774.1"
                     /translation="MRTKAELDAMSHQELKDYEQSLLALWTPRMAIESDIERLSTHHSE
                     LLEVFNQLKNPDAPKNSRLKDSILSLKYKIESLEGKLSDLIQDNRLNSAD"
                     /name="hypothetical protein"
     gene            complement(8840..9781)
                     /locus_tag="BEN74_RS00645"
                     /old_locus_tag="BEN74_00640"
     CDS             complement(8840..9781)
                     /locus_tag="BEN74_RS00645"
                     /old_locus_tag="BEN74_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004729701.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_020846814.1"
                     /translation="MDENKKTYPLSWVVMQNNVQECFKSMNIDEKRMLILASPIARTTD
                     ATEKDRIMITAEEFARECGIKTHSAYTQLELASKNLLKRNFSYNNERGKKVLSNWVIDC
                     TYEDGGIAIRFPEIVLLMLKEFDKLNPYTKYKKDIVLSLKKDYSFDLYHLAKKHQAMGQ
                     FEMSLEHIKNELGLPKSYDKLCNLKDRVMKPSLNEITTNTDIELTYENVKKGRSVVGFK
                     FIVKEKPKPKLIAPQRDQRTIDMFCNLSDAQINKYSTILSKLSELSDLSNFPDYPTFAL
                     WISGILRDPKSVREETTKRIFKALHSKTDFKP"
                     /name="replication initiation protein"
     gene            complement(10492..12144)
                     /gene="mobQ"
                     /locus_tag="BEN74_RS00650"
                     /old_locus_tag="BEN74_00645"
     CDS             complement(10492..12144)
                     /gene="mobQ"
                     /locus_tag="BEN74_RS00650"
                     /old_locus_tag="BEN74_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005199240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_068913314.1"
                     /translation="MAIYHFSVKTISRGNGRSAVACAAYRSSEKLVCDFYGKEQDYTRK
                     TGVEFTKIYAPENTNTELLKRQTLWNEVEKVERRKDALLAREFEIAFPSELNAEQRENM
                     LNELCRNLVKKYGVIVDAAIHAPHTDSGSDERNYHAHIMFTTRSINEQGGFSAKKYRDF
                     SRDNGTETVSHWRESFAELCNHHLEQNGFDERVDHRSYEDQESDLEATQHEGPQATYLR
                     RRGIFTEISLKNDEIKLRNLKIKKVIALDENIKVSEDLVQKVRSELQFQYSQAEYSEKT
                     SQKLYEFQNEELSKLTTKLDTMSSAISELRKKEPLFFKTKWINQINDLIDQHNTQLDIQ
                     KHISGLSEIEKKERYSDHLNKWTEENQLLQPKKSFAKLDEPNITVKQRKLNDQISNIDH
                     QISEISRRETEYQEYVRDQRLEIINSYIFEEDNYGNKYFSPQNGEKQKRLYAEEMEDLK
                     IQELHAAFTKKIETEAQQEFSQKRAIQLENDRKDRESREQQLRKEREQQEQRYAQEREQ
                     QEHLAFVRRQEQEKQQRNEPKKPENDNNHDYTP"
                     /name="mobQ"
     ARG             complement(7955..8298)
                     /name="qacG"
                     /gene="qacG"
                     /gene_name="small multidrug resistance (SMR) antibiotic
                     efflux pump"
                     /tool="rgi"
                     /drug_class="disinfecting agents and antiseptics"
                     /identity="48.11"
                     /coverage="106.54"
     Typing          complement(3580..4529)
                     /name="rep_cluster_1171"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.79"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(5423..6864)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.792"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(10703..12144)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.792"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gtttctgatc agcaacgtaa agatgatacg agacgtaaaa ttctactggg agcttgggct
       61 ttaaataaat taaaaaatga tgaatcgttt aaacagcaat tagccgattt tgagcaattt
      121 ttaaatacgg aaaataagac cgaagaaaat aagcagaaag ataaagatct ttttaatgga
      181 tttatttggg ataagaccta aatttcgcat aagagatttt atgttaaata agatggaact
      241 tagacaaaat tttattaata gttatgcatt ctgcacagtt agttttagac ctaaagcatt
      301 tactacacga ttaatcgtat caaaacgagg tgctgaatct tgacggagtg ctttgtaaag
      361 tgcttcacgt ccaattcctg cttctttagc gatttgagtc ataccacgag ctttagcaat
      421 tacaccaagt gcatgagcta gttcagcagg gtcattctct tcaagaacca tattaagata
      481 cattacaata tcttcttcac tcttaagtgc ttctgccatg tcaaagctag gtagatcagc
      541 tattttaacc atttttcaat cctctaaaga ttttgataaa tcaacagctc gttctatatc
      601 cttttgctgt gtagatttat caccacctcc aagcatcaca atgattacat caccatgttt
      661 gatgtaatac atgcgccaac ctggtccgaa gaactctcgc atttcccaaa caccttcttg
      721 aagatgcttt acatcgcccc aattaccacg ctgaacctta tctaatcttc gattcagttt
      781 aagacgggtc atactgtctt taatgctatc tagccattca tcaaattcag ggagacgttt
      841 aatctcggtc atgaaagttc ctctttattg tattcgatcg aatacacaac attataatga
      901 atatatttta tgagtaaatg aaataataag aatatatttt atcaattgat tatttaataa
      961 cctatgttct tggcaaaagg tatcaaacct gctcataaaa aagccgactt gtttctaagt
     1021 cggcttttta ctgtttttga agtcagtcaa aattttataa ctatatgaaa aacaataata
     1081 ttttaaattg cgtttcgtat aagctctatt atgttaaata attaaggata aaagctttat
     1141 tactaaatag aacagttgct taaagcagta tagagttaag tttgcatttt aatatcaagt
     1201 tgtctttcag caccgataat caccatacga atcccatttt caaaaatgtc atccgcattc
     1261 atattttgta aggtattcac tccttggagc atgagtggat atatagttgg atcggcttcg
     1321 aatggtgggt ctgatcttat ttcttcttgc tgctcctcaa ggacccaccc aacaataaac
     1381 cttcctaatg tcataagtaa tgcaactgct tcaacaggag aaaacccagc atgagataac
     1441 agcgcaactt gggcctcaat cgtgttgaaa tgtccttgac tggggctagt tcttgcatgt
     1501 agacgagcac catcacggta agctagtagt gctcgacgaa agcttacaga attagcgagc
     1561 aaccaatctt gccatgtcat cccatcgatg ggtaaagaaa ctaaatggtg ttcatttata
     1621 atggtttccg ccattgcttc cattaacaag gatttattct tgaaatgcca atatagagtt
     1681 ggagatttaa tacccaatcg ttgagctagc ttgcgtgtgg ttattgaatc tatgccttct
     1741 tcattcaaca tatccaatgc ggtacgcagg actatttcac gtgtcagacg ttgaggtgca
     1801 atatcttttt tactcataaa tctatcactg atagggagtg tgtgagagaa tattaattct
     1861 atcattgcta gagaaggcat ttgtgaagaa atcattgagc gtgattttaa tcactatatt
     1921 tctggatgct gttgggattg gtttaattat gccgatcttg cctgaattat tacggtcatt
     1981 ggctggagct gaagcaggcg gtgttcacta tggtgcttta ttagctgtgt atgctctgat
     2041 gcagttcatt tttgcaccta tccttggagc gttgagtgac cgatttggac gtcgacctgt
     2101 attaattatt tcaattgctg gtgcaacggc tgattatctc ctaatggctg ctgctccttc
     2161 tctattgtgg ctatatattg gtcgtatttt tgcgggaatt acaggtgcca acatggctgt
     2221 tgcaacagct tatgtttcag atattactcc agcccatgag cgtgcaaaaa ggtttggtct
     2281 ccttggagct gtctttggta ttgggtttat agcgggtccg gtaataggtg gagttttggg
     2341 tgaatggaac ttacatgcac cgttctttgc tgctgctttt atgaatggga ttaatttaat
     2401 aatgacagca gtcttattaa aagaatcaaa acacagcaat aaaatgactg agaaggttca
     2461 ggagcaatca atattaaaga aattatccta tttgatcact caacctaata tggctccatt
     2521 gcttggtatc tttttaatta tcacattggt ttcacaagtc cccgcaactt tatgggttat
     2581 ctatgggcag gatcgttatg gctggagtat atttattgca ggtgtttccc ttgctagtta
     2641 tggaatatgc cattctattg cacaggcttt tgctatcgcc cctatggtaa agaggtttgg
     2701 agagaaaaat acgttgttat gtggaatagc ttgcgatgca attggtttac ttcttttatc
     2761 tattgctgtt gaagaatggg tgccttttgc gttgttacca ttgtttgccc ttggtggagt
     2821 agccgttcct gctttgcaag caatgatgtc cagaggtatt agtgatgaaa gacaaggtga
     2881 attacaaggg ctattaagca gttttaatag tctgggggct ataattggtc ctgtattagt
     2941 tactagcctc tattttatga ctcaggcatc agctcctgga atggtatggg cattagctgc
     3001 aatactttat gtaatcaccc tacccttatt gcttaagtat cgcctgaata aatattctgg
     3061 agttccataa aatatttaat ttattggttt aaaaactaat ggattctata gtttatctaa
     3121 aattaacata atacacctta tacgaaatac tgttttttat tcgatataaa tcataaactt
     3181 atactaagac gttccagggt tcagcgcact ggaacggctt ttttgtgact attcacgttc
     3241 cacgcctatt ctttgatcaa tgtttcacgg ttttaattaa ataaactgaa taattcaatc
     3301 tgcacgattt aagcgattat cttgaataag atcatccaat ttatcttcta aatcttcaat
     3361 cttatatttg agcgataaaa tagaattttt taaacgctca ttttcaggag catcaggatt
     3421 ttttaattga ttaaaaattt ctaataattc gttgcgatta gtgcttaacc tttcaatgtc
     3481 actttcaata gccattcttg gtgtccacag agcaagtaaa ctttgctcat agtctttgag
     3541 ttcttgatgg ctcattgcat ctaattcagc tctagttttc atggtttaaa atccgtcttg
     3601 ctatgaagag ctttaaaaat ccgctttgct gtttcctctc ttacactttt cggatctcgt
     3661 aagatgccac taatccaaag ggcaaaacta ggatagtcct ggaagttact taggtctgat
     3721 agctcagaaa gtttagataa aatagcactg tacttgttaa tttgagcatc agacaggttg
     3781 cagaatatat ctattgtttt tggatctcgc tcaggagcta ttaattttgg cttagatttt
     3841 tctttcacag tgaacttaaa gccgacaaca gaacgtcctt ttttaactgg actatagtca
     3901 actgttacat cggtaaattc attaatttca tctaatgagc tcttcaaaac tcttcgtttt
     3961 aagttcctca aatctctata agattctggt aaaccaaact ctgtaaacat ttcatctagc
     4021 gttaattcaa agccattttt tgcctgattt tttttcgcta aatggtaaaa gtctattgag
     4081 tagtcttttt ttaattttag aactacatct tttttataac gagtgtatgg attgtattta
     4141 tctaaaattt tcaacatcac caaaacaata gatgtaaacc ttaaagatat ccctgcatct
     4201 tcataagtag catcaattac ccagttagag tagacctttt tccccctatc attaacgtat
     4261 gaaaaggacc gatctactag tttttttgac gcattttcaa tttgtttata agcagaattg
     4321 actttgatac cacagtcatc agcaaattgt tgagcagata ttaagatttg atcttgttca
     4381 cttgcatcga tatttctagc aatcggagaa gccataatta aaattctttt ttcatctaca
     4441 gacatgcttt taaagcattc agtaactttg ttttgaatag ccattgttgt tactggataa
     4501 gatttatcat aactattttc tttattcatt cagatatcaa accttataaa aaatgaacat
     4561 gatttttgta cccttatgag aaaacaatgt acaccaataa acataagggt acaataattt
     4621 atttgtaccc tttatcatgc tatttgtacc ctttaatcat gctatttgta cccttttaaa
     4681 cgataaaatc atgctatttg taccctttat catgctattt gtaccctttt aacgcttgaa
     4741 actattgctg cataaggttt tcagagggtc taaaagcttt taaaagcttt ttaaaagaat
     4801 aaaagggaat ttatttccaa agtgagttat taaaactgcc cttggtactc gctaaagctc
     4861 gtactctatt gaagctcgtt tcactcgctt cgtgggggca gttttttgca tatatgatca
     4921 aggattcaaa aagcaaaaca tgaataatgc attcgctatg ctcatgcttt ttgacgagca
     4981 aaagcatcaa tacttcgctc gtagacactc gctaatcact gtccccagta attcaataaa
     5041 aaacggtttt tcagaggggg aataagcaac ctttcgttgt gtcgctcgta gacactcgct
     5101 aaaaggctct catttgcgtt ctaagcgatt ttatttttat caactatagt tttactgtat
     5161 ttctatcaaa agtcgctaaa acccccgttt ttttgcgctt aaatgggatt tctaaggcgt
     5221 gtagtcatgg ttattgtcat tttctggctt tttaggctca tttctctgct gtttttcttg
     5281 ttcttggcgt cttacaaatg ccaaatgttc ttgttgctct cgttcttgtg catagcgttg
     5341 ttcttgttgc tctcgttctt tacgtaactg ctgttctcta gattcacgat cttttcggtc
     5401 gttttcgagc tgtattgctc ttttttggga aaactcttgc tgcgcttcgg tctcaatttt
     5461 ttttgtaaaa gccgcatgca attcttggat ttttagatct tccatttctt ctgcatatag
     5521 tcgcttttgt ttttcaccat tttgaggact aaagtattta tttccatagt tatcttcttc
     5581 aaatatataa ctatttatga tctccagtct ttgatcgcgt acatactctt gatactcagt
     5641 ttctctgcgt gatatttcag aaatttgatg atctatattt gaaatttgat cattaagttt
     5701 tcgttgttta acagtgatgt tcggttcgtc aagttttgca aaagactttt taggctggag
     5761 cagctgattt tcttctgtcc atttatttaa atgatcagaa tatctctctt ttttttctat
     5821 ttcactaaga ccagaaatat gcttttggat gtctagttgg gtgttgtgtt ggtcgatcag
     5881 gtcatttatt tgattgatcc attttgtttt aaaaaataaa ggctcttttt tgcgtagttc
     5941 tgatattgca gaactcatcg tgtctaattt tgttgttaat tttgataatt cttcattttg
     6001 gaattcataa agtttctgag aagttttttc tgaatactca gcttgagagt attgaaactg
     6061 aagctcactt cgtacttttt gaacaagatc ctcagatact ttgatgtttt catccagtgc
     6121 gatgaccttt ttgattttta aattacgtag cttaatttca tcatttttca gactaatttc
     6181 tgtaaatatc cctcgtctcc ggaggtatgt cgcttgagga ccttcgtgct gagttgcttc
     6241 taggtcactt tcctggtctt cataacttct gtgatcgaca cgttcatcaa atccattttg
     6301 ttccaggtga tgattgcata actctgcaaa tgattctctc cagtggctta cagtttctgt
     6361 gccattgtca cgactgaagt ccctatattt ttttgctgaa aagccaccct gctcattaat
     6421 acttcgggtt gtgaacatga tgtgtgcatg gtagttgcgt tcatcacttc cactgtctgt
     6481 atgaggtgca tgaatagcag catcaacgat gacaccgtat tttttaacga ggtttcggca
     6541 aagttcattg agcatatttt cacgctgttc agcgtttaac tcactgggaa atgcaatctc
     6601 aaactctctt gcaagtaatg catcttttct acgttcaacc ttttcgactt cattccaaag
     6661 tgtttggcgc ttgagtaact cagtatttgt attttcaggt gcatagattt ttgtaaattc
     6721 gacaccagtt tttcttgtgt agtcttgttc ttttccataa aaatcgcata ccaatttttc
     6781 acttgaacga tatgcagcac aagcaacggc cgatcgtcca ttacctctac taatcgtttt
     6841 aactgaaaaa tgatatatcg ccattttgct tttgctcttt atttcgattt atcgaaatta
     6901 ccaacatgta tatgttggtc aggggattac aaaggggatt taccccttgt cgcaccattg
     6961 gatgaaatcc aatggctaag tgcgcccttc gggaaaacca cgatagcatg ggctgtcttg
     7021 aatttcaatt caaacagacc tatgcttgag ggggattcct tgagatggtg atggtatgag
     7081 tgttgaaact ttagagcaaa aaattgcaaa gcaagaagaa cgattaagac aactaaaagc
     7141 acaaaaacaa gctattgctg ctcgtgaaaa aaagaaaaat tctgatcgac aaagaaaaga
     7201 tgatactaga cgtaaaattc tactgggggc ttgggttcta aacaaactga aaaatgatga
     7261 atcttttaag ggtcaactaa ccgattttga aaagttttta agcacggaaa gtaagactga
     7321 agaaaatagg cagaaagata aagatctttt taatggattt atttgggata agacctaaat
     7381 ttcgcataag agattttatg ttaaataggt tcgattaagc agatcgtctt ctaattttga
     7441 acattgctca tcatcaatga ctccggtaat actttgtagc cacaacccat ctgctgctaa
     7501 acgaagtaac tctaaatcag gatgactatc cgtttctgca tgttgagata atctattttt
     7561 tagccattcc atccaaattt tattaaaggt aggctctgtg atcatagtca ttgataacgc
     7621 tgaccaagca gaaaccaccc catctatttg atggataaaa gaactatgaa tataagctcg
     7681 tgtgaattta ccatattctg tttgatcgcg tttaatcaag tattcaactg tttgatctaa
     7741 atcaagaatt atttgattga acatttcttc tatcaaattt tttttattcg ggaaatggtg
     7801 aaataccccc ccttttgtta tacctactgc tgtagcaata ttttgaatag aaacaccaga
     7861 aatacccttt tgagaaacaa gaatagttgc ctgttcaagg atgcgctttc gaataacctc
     7921 tggttgcttc ttttgttgcc gaatttcttt cacttaatga cctgctgatt gcgaaaataa
     7981 attaataatt attacgccag atacgataaa accaattcca attaaagcag gattatctag
     8041 tgtctgttta aaaacaaagt agctaactac agcagttaga acaatcccaa caccagccca
     8101 tatagcgtat gcgataccta aagggattgt tttaataact tgtgataata aataaaatgc
     8161 cgtgctaaaa aaaataaaga ccatcacgct tggaaaaagt tttgtaaacc cttccgattt
     8221 aactaaaaaa ctacttccaa tcacttcgga aactattgaa aatccaagta atacataagc
     8281 gagtaacaca ctgttcataa gtaatcatac ctaaaatacc catcggaagg tatgttaaat
     8341 tttcaaaaaa aaattatcaa taattatttt agattttcta aaattaacat aatacacctt
     8401 atacgaaatg cgttataatt atccttataa ttcaacgctg tagtttcaat tttctagccc
     8461 agtcgtcaat gactgggctt tttttattac ttttcacgtt ccacgcctag tctttaatca
     8521 atgttccacg attttgatta aataaactga ataattcaat ctgcagaatt taagcgatta
     8581 tcttgaataa gatcactcaa cttaccttca agcgattcga ttttatattt cagagacaag
     8641 attgaatctt ttaaacgtga atttttaggt gcatccggat tctttaattg attaaaaacc
     8701 tctaataatt cgctgtgatg agtgcttaac ctttcaatgt cactttcaat agccattctt
     8761 ggtgtccaca gagcaagtaa actttgctca taatctttga gttcttgatg actcattgca
     8821 tctaattcag ccttagttct catggtttaa aatccgtctt gctatgaaga gctttaaaaa
     8881 tccgttttgt tgtttcctct cttacacttt tcggatctcg taagatgcca ctaatccaga
     8941 gggcaaatgt cgggtagtct gggaaattac tgaggtctga tagctcagaa agtttagata
     9001 aaatagtgct gtacttgtta atttgagcat cagataaatt gcagaacata tctatagttc
     9061 tttgatctcg ctgaggcgct attaattttg gcttaggttt ttctttcact ataaatttaa
     9121 agccaactac tgaacgccct ttttttacat tttcataagt gagttcaata tctgtattag
     9181 ttgtaatctc atttaatgaa ggcttcatta ctcggtcttt taaattacag agtttgtcat
     9241 aagatttggg caaacccagc tcatttttta tatgttctaa ggacatttca aattgtccca
     9301 ttgcttgatg ctttttagct aaatgataaa gatcgaatga gtaatctttt ttcaaactta
     9361 gtacgatatc ttttttatat tttgtataag ggtttaattt atcaaactct tttaacatca
     9421 gtagaacgat ttcagggaaa cgtatagcta tccctccgtc ttcataagtg caatcaatca
     9481 cccaattcga cagcaccttt tttcctcttt cattgttata agagaaattt cttttaagta
     9541 gattcttgct ggctagttct aactgcgtat atgcggaatg cgttttaatg ccacattctc
     9601 tagcaaactc ttctgccgta atcataattc ggtctttttc agttgcatca gtggttctag
     9661 caattgggct agcaagtatt aacattcgtt tttcatcaat gttcatactc ttaaagcatt
     9721 cttgaacatt gttttgcata acaacccagc tcagaggata tgttttttta ttttcgtcca
     9781 tataactaca accccaaaaa actaactaac tacattggta tttatgtagc ttaaacatac
     9841 attgtcgttt attttttgac tactattttt gtagtttttt aacaaaacta caaacttttt
     9901 cttgtagttt aatgtccggt aaaagtagtt tctatgtccg gtaaaagtag ttttttaagt
     9961 ccattttgtc cggcatttgt agtttaatgt ccggtaaaag tagtttttta aagtctgaaa
    10021 gcattgctag gcgtggttta taggggggtc taaaagcttt taaaagcttt ttaaaagaat
    10081 aaaagggaat ttatttccaa agtgagttat taaaactgcc cttggtactc gctaaagctc
    10141 gtactctatt gaagctcgtt tcactcgctt cgtgggggca gttttttgca tatatgatca
    10201 aggattcaaa aagcaaaaca tgaataatgc attcgctatg ctcatgcttt ttgacgagca
    10261 aaagcatcaa tacttcgctc gtagacactc gctaatcact gtccccagta attcaataaa
    10321 aaacggtttt tcagaggggg aataagcaac ctttcgttgt gtcgctcgta gacactcgct
    10381 aaaaggctct catttgcgtt ctaagcgatt ttatttttat caactatagt tttactgtat
    10441 ttctatcaaa agtcgctaaa acccccgttt ttttgcgctt aaatgggatt tctaaggcgt
    10501 gtagtcatgg ttattgtcat tttctggctt tttaggctca tttctctgct gtttttcttg
    10561 ttcttggcgt cttacaaatg ccaaatgttc ttgttgctct cgttcttgtg catagcgttg
    10621 ttcttgttgc tctcgttctt tacgtaactg ctgttctcta gattcacgat cttttcggtc
    10681 gttttcgagc tgtattgctc ttttttggga aaactcttgc tgcgcttcgg tctcaatttt
    10741 ttttgtaaaa gccgcatgca attcttggat ttttagatct tccatttctt ctgcatatag
    10801 tcgcttttgt ttttcaccat tttgaggact aaagtattta tttccatagt tatcttcttc
    10861 aaatatataa ctatttatga tctccagtct ttgatcgcgt acatactctt gatactcagt
    10921 ttctctgcgt gatatttcag aaatttgatg atctatattt gaaatttgat cattaagttt
    10981 tcgttgttta acagtgatgt tcggttcgtc aagttttgca aaagactttt taggctggag
    11041 cagctgattt tcttctgtcc atttatttaa atgatcagaa tatctctctt ttttttctat
    11101 ttcactaaga ccagaaatat gcttttggat gtctagttgg gtgttgtgtt ggtcgatcag
    11161 gtcatttatt tgattgatcc attttgtttt aaaaaataaa ggctcttttt tgcgtagttc
    11221 tgatattgca gaactcatcg tgtctaattt tgttgttaat tttgataatt cttcattttg
    11281 gaattcataa agtttctgag aagttttttc tgaatactca gcttgagagt attgaaactg
    11341 aagctcactt cgtacttttt gaacaagatc ctcagatact ttgatgtttt catccagtgc
    11401 gatgaccttt ttgattttta aattacgtag cttaatttca tcatttttca gactaatttc
    11461 tgtaaatatc cctcgtctcc ggaggtatgt cgcttgagga ccttcgtgct gagttgcttc
    11521 taggtcactt tcctggtctt cataacttct gtgatcgaca cgttcatcaa atccattttg
    11581 ttccaggtga tgattgcata actctgcaaa tgattctctc cagtggctta cagtttctgt
    11641 gccattgtca cgactgaagt ccctatattt ttttgctgaa aagccaccct gctcattaat
    11701 acttcgggtt gtgaacatga tgtgtgcatg gtagttgcgt tcatcacttc cactgtctgt
    11761 atgaggtgca tgaatagcag catcaacgat gacaccgtat tttttaacga ggtttcggca
    11821 aagttcattg agcatatttt cacgctgttc agcgtttaac tcactgggaa atgcaatctc
    11881 aaactctctt gcaagtaatg catcttttct acgttcaacc ttttcgactt cattccaaag
    11941 tgtttggcgc ttgagtaact cagtatttgt attttcaggt gcatagattt ttgtaaattc
    12001 gacaccagtt tttcttgtgt agtcttgttc ttttccataa aaatcgcata ccaatttttc
    12061 acttgaacga tatgcagcac aagcaacggc cgatcgtcca ttacctctac taatcgtttt
    12121 aactgaaaaa tgatatatcg ccattttgct tttgctcttt atttcgattt atcgaaatta
    12181 ccaacatgta tatgttggtc aggggattac aaaggggatt taccccttgt cgcaccattg
    12241 gatgaaatcc aatggctaag tgcgcccttc gggaaaacca cgatagcatg ggctgtcttg
    12301 aatttcaatt caaacagacc tatgcttgag ggggattcct tgagatggtg atggtatgag
    12361 tgttgaaact ttagagcaaa aaattgcaaa gcaagaagaa cgattaagac aactaaaagc
    12421 acaaaaacaa gctattgctg ctcgtgaaaa aaagaaa
//
