LOCUS       NZ_CP032271            13204 bp    DNA     circular CON 05-JAN-2024
DEFINITION  Acinetobacter sp. WCHAc010034 plasmid p3_010034, complete sequence.
ACCESSION   NZ_CP032271
VERSION     NZ_CP032271.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN05356835
            Assembly: GCF_001696615.3
KEYWORDS    RefSeq.
SOURCE      Acinetobacter sp. WCHAc010034
  ORGANISM  Acinetobacter sp. WCHAc010034
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter.
REFERENCE   1  (bases 1 to 13204)
  AUTHORS   Hu,Y., Long,H., Feng,Y. and Zong,Z.
  TITLE     The complete genome of Acinetobacter sp. strain WCHAc010034
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13204)
  AUTHORS   Hu,Y., Long,H., Feng,Y. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2018) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
REFERENCE   3  (bases 1 to 13204)
  AUTHORS   Hu,Y., Feng,Y. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAR-2017) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.6
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology             :: Illumina HiSeq; MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/04/2024 21:32:37
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,697
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 112
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 21 of 68
            Pseudo Genes (incomplete)         :: 53 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 16 of 68
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP032271.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..13204
                     /organism="Acinetobacter sp. WCHAc010034"
                     /mol_type="genomic DNA"
                     /strain="WCHAc010034"
                     /isolation_source="hospital sewage"
                     /db_xref="taxon:1879049"
                     /plasmid="p3_010034"
                     /geo_loc_name="China"
                     /collection_date="2015-10-31"
     gene            1..954
                     /gene="repM"
                     /locus_tag="BEN74_RS00505"
                     /old_locus_tag="BEN74_00505"
     CDS             1..954
                     /gene="repM"
                     /locus_tag="BEN74_RS00505"
                     /old_locus_tag="BEN74_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004786597.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepM"
                     /protein_id="WP_068912084.1"
                     /translation="MRELVVKDNALINASYNLDLVEQRLILLAIVEARESGKGINANDP
                     LEVHAEGYINQFGVHRNTAYQALKDACNDLFARQFSYQKINERGNIENYRSRWVSEIGY
                     VDNEAVVKLIFAPAIVPLITRLEEHFTKYELQQVSNLSSAYAVRLYELLIAWRSIGSTP
                     VIELSDFRQRIGVLDTEYKRMERFKTSVLELALKQINEHTDITVKYEQHKKGRSIYGFS
                     FTFKQKKVDAPSVGNKDPNTIDLFSKLSDSQRYLFANKLSELPEMGKYSQGTESYQQFS
                     IRIAEMLQDPERFKELYPYLKKVGYMPSNKKDTVNG"
                     /name="repM"
     gene            947..1522
                     /locus_tag="BEN74_RS00510"
                     /old_locus_tag="BEN74_00510"
     CDS             947..1522
                     /locus_tag="BEN74_RS00510"
                     /old_locus_tag="BEN74_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005102333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication DNA-binding protein"
                     /protein_id="WP_068912082.1"
                     /translation="MAKLSLSEVSKQFHVDRSTIYRAVRNGRLSRSSNGQFDLSEVIRC
                     FGEPAQASQQKVTPKQESDASTQKLITHLENEVKKYQEREERLMQQIDRMQTLIELKSV
                     APATAVPQQDATACDTSMPRHATSNQINENKEYLYKNMTENVAVPQQDATACDTSTPQH
                     ATLQNVAMPQSKKRGLFGRVFSAVFNEN"
                     /name="plasmid replication DNA-binding protein"
     gene            1546..1689
                     /locus_tag="BEN74_RS19170"
     CDS             1546..1689
                     /locus_tag="BEN74_RS19170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004676226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_162898102.1"
                     /translation="MKKLKEVLQGIAFLLAGIPIMIIASIMDQKAFKKEREQRQKAQEQ
                     SK"
                     /name="hypothetical protein"
     gene            2035..3000
                     /locus_tag="BEN74_RS00515"
                     /old_locus_tag="BEN74_00515"
     CDS             2035..3000
                     /locus_tag="BEN74_RS00515"
                     /old_locus_tag="BEN74_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005227841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="WP_068912079.1"
                     /translation="MNTLQEYCKPYISPQKLVLDYLISKKGLAIQDNEIEFAEQALSQI
                     NWYHLKIYFYPFIEDLTAEEERYKPQTNFRNGWDIYLLDDELRKIVIKHTLKIELIVKS
                     HIDQAITEFTEDPFWYLNDDYFIKNKQPYYERNKVLGSMNSSDAEFTKHFQKNYKSPYK
                     SYRSLPPFWIAMEVITFDQFLKIIEKVNPQIFEKRGENALDKCAMKLGAENFKQLKSWL
                     EVIKDIRNKGCHNSRLWNANHMIPKGLDIHSQPIESLSKPHKIYLVTLAIFLMTKNSVA
                     IDKNIKEELEELFEAYGSKINDLEKQMGFPESWATKAIWT"
                     /name="Abi family protein"
     gene            complement(3265..3963)
                     /locus_tag="BEN74_RS00520"
                     /old_locus_tag="BEN74_00520"
     CDS             complement(3265..3963)
                     /locus_tag="BEN74_RS00520"
                     /old_locus_tag="BEN74_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004878081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4145 domain-containing protein"
                     /protein_id="WP_068912077.1"
                     /translation="MSSFPWKCPYCNHTCTVTSENYSDSTHRFRLNNKYDRLVGVNTII
                     VVCPNPECKEISAKALLFKSATTYDYTPEEKPEKTWNLLPQSSSKTYSTAIIPEVIIND
                     YEEACLIKDLSPKASATLARRCLQGMIRDFWNVKEKNLFEEIKAIENKIEPEVWEAIDS
                     IRSLGNIGAHMEKDINIIVDVEPDEAQLLISLLEMLFDEWYIAAHKRKERLKKIKELKA
                     SKDHLKKVEY"
                     /name="DUF4145 domain-containing protein"
     gene            4185..5303
                     /locus_tag="BEN74_RS00525"
                     /old_locus_tag="BEN74_00525"
     CDS             4185..5303
                     /locus_tag="BEN74_RS00525"
                     /old_locus_tag="BEN74_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004755996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="WP_068912092.1"
                     /translation="MQEWIWQAENWTNFTWLDSIILPKTRQIHQKIGILLGQSQHDLAK
                     EQFTLDTLLANLVASSAIENETINVFSLRSSLAQRLGVTLEQPYPSSDRSEGLANIMLD
                     ALNDVKQPLTVERLMQWHRWLFNDPDWTMQRLRIGQLRGSEPMQVVSGRLDYPKVHFEA
                     PPREGLEERLNTFIAWFNQSLNDSLLDPLLRAGITHLWFVTLHPFDDGNGRITRTLTDL
                     ALAQMDEQSIRLYAMSTAILNKRKSYYEILEQTQKNDSDITDWLVWFLDTLNDSLEKTL
                     AQINRILFKSQFWHKYSNLALSEEQRKVLNRLLDGGENGFEHGISASQYQKVAKTSKAT
                     ATRHLSDLLEKECIVKLEGGGRNTRYQINTQL"
                     /name="Fic family protein"
     gene            complement(<5823..5957)
                     /locus_tag="BEN74_RS19625"
                     /old_locus_tag="BEN74_00530"
                     /pseudo=""
     CDS             complement(<5823..5957)
                     /locus_tag="BEN74_RS19625"
                     /old_locus_tag="BEN74_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008843103.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /name="NAD(P)/FAD-dependent oxidoreductase"
     gene            complement(5983..6816)
                     /gene="fghA"
                     /locus_tag="BEN74_RS00540"
                     /old_locus_tag="BEN74_00535"
     CDS             complement(5983..6816)
                     /gene="fghA"
                     /locus_tag="BEN74_RS00540"
                     /old_locus_tag="BEN74_00535"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000439570.1"
                     /GO_function="GO:0018738 - S-formylglutathione hydrolase
                     activity [Evidence IEA]"
                     /GO_process="GO:0046292 - formaldehyde metabolic process
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="WP_068912072.1"
                     /translation="MERIEHHASFEGWQDVFQHESTVLGCMMRFGIYLPPQAEHEKLPV
                     LYWLSGLTCTEQNFITKASVQRYAAEHGIIVVAPDTSPRGESVADDAAYDLGQGAGFYV
                     DATELPWSDHYQMYSYVVDELPALIEANFPASSRRSISGHSMGGHGALVIALRNPGRYA
                     SVSAFSPIVAPTQVPWGQKALAAYLGEKNLDAWKQYDTVELIHVAQERLPLLVDQGLSD
                     EFLEHQLRPQLLRTACEQVGHPLTLNLRPGHDHSYYFISTYLSEHMAHHAAALNR"
                     /name="fghA"
     gene            complement(7287..7685)
                     /locus_tag="BEN74_RS00550"
                     /old_locus_tag="BEN74_00545"
     CDS             complement(7287..7685)
                     /locus_tag="BEN74_RS00550"
                     /old_locus_tag="BEN74_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009639360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="WP_068912070.1"
                     /translation="MAIPVISGNDIFSHVFLGAVDLNKSVAFYDATLGALGIKNLGPFG
                     NDWVLYGRDKPAFMIARPGNGAEPTSNGVTVGFAAATPAEVNAFHAAGLAAGGTDEGQP
                     GPRGHLPGAYAAYLRDPAGNKVCAYTFV"
                     /name="VOC family protein"
     gene            complement(7738..8847)
                     /locus_tag="BEN74_RS00555"
                     /old_locus_tag="BEN74_00550"
     CDS             complement(7738..8847)
                     /locus_tag="BEN74_RS00555"
                     /old_locus_tag="BEN74_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308438.1"
                     /GO_function="GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA]; GO:0051903 -
                     S-(hydroxymethyl)glutathione dehydrogenase activity
                     [Evidence IEA]"
                     /GO_process="GO:0006113 - fermentation [Evidence IEA];
                     GO:0009404 - toxin metabolic process [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase/class
                     III alcohol dehydrogenase"
                     /protein_id="WP_068912067.1"
                     /translation="MKSRAAVAFGPGKPLEIVEIDVEPPRKGEVLVKITHTGVCHTDAF
                     TLSGDDPEGIFPVVLGHEGAGIVVEVGEGVTSVKPGDHVIPLYTAECGECLFCKSGKTN
                     LCVAVRATQGKGLMPDGTTRFSYNGQPLFHYMGCSTFSEYTVVAEVSLAKINPDANPEH
                     VCLLGCGVTTGIGAVHNTAKVQPGDSVAVFGLGGIGLAAIQGARQAKAGRIIAIDTNPS
                     KFELARSFGATECINPKDYEKPIHEVLIEMTGWGVDHTFECIGNVNVMRSALEAAHRGW
                     GQSIIIGVAGAGKEISTRPFQLVTGRTWKGSAFGGVKGRTQLPGMVEDAMKGEIDLAPF
                     VTHTMGLDEINDAFDLMHEGKSIRTVIHY"
                     /name="S-(hydroxymethyl)glutathione dehydrogenase/class III
                     alcohol dehydrogenase"
     gene            complement(8869..9144)
                     /locus_tag="BEN74_RS00560"
                     /old_locus_tag="BEN74_00555"
     CDS             complement(8869..9144)
                     /locus_tag="BEN74_RS00560"
                     /old_locus_tag="BEN74_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001133625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal/formaldehyde-sensitive transcriptional
                     repressor"
                     /protein_id="WP_068912065.1"
                     /translation="MPNQVEDKKKILTRVRRIKGQAEAIEKALESNVECNAILQQICSV
                     RGAINGLMNEMLEVHLKDTLVSGETTEQQRNEELAEIAKILKSYLK"
                     /name="metal/formaldehyde-sensitive transcriptional
                     repressor"
     gene            complement(9256..10406)
                     /locus_tag="BEN74_RS00565"
                     /old_locus_tag="BEN74_00560"
     CDS             complement(join(9256..10166,10166..10406))
                     /locus_tag="BEN74_RS00565"
                     /old_locus_tag="BEN74_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612015.1"
                     /ribosomal_slippage=""
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="WP_228200337.1"
                     /translation="MSSSKRYPEEFKIEAVKQVTEKGHSVAEVAARLGTTTHSLYAWIK
                     RYAPQEPKITDSRDAVSELAKLKKELQRVTEERDIFKKSRGVLRKPVQMRYAFIQDNQH
                     IWSVRRLCSTLDVHHSGYYAWLKQPTSKTARKRQQLSGLIKQFWLESGGVYGYRKIHCD
                     LKDVGENCGINRVHRLMKANGLKSQRGYRKPRSYAGTPSIVSANTLERQFNPTQPNRKW
                     VTDITYIRTHEGWLYLAVVIDLFSRLVVGWSMKSRMTTDLVLDALLMALWRRKPKNKVL
                     IHSDQGSQYTSHEWQAFLKQHNLECSMSRRGNCHDNAVAESFFQLLKRERVKKRNYASR
                     TEARSDIFEYIEMFYNSKRRHGSNGQRSPLDYEKSHQKMVMCV"
                     /name="IS3 family transposase"
     gene            complement(10590..10880)
                     /locus_tag="BEN74_RS00570"
                     /old_locus_tag="BEN74_00565"
     CDS             complement(10590..10880)
                     /locus_tag="BEN74_RS00570"
                     /old_locus_tag="BEN74_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004647750.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antidote protein"
                     /protein_id="WP_005093398.1"
                     /translation="MVKVADLPSFDMAESLKTEEDIVMYLNMVLEENDPAELAHALGVI
                     AKARGMTQIAKEAGIGREALYKALRHDSAPRFDTINRVVNALGLKLTVQHA"
                     /name="addiction module antidote protein"
     gene            complement(11207..11530)
                     /locus_tag="BEN74_RS00575"
                     /old_locus_tag="BEN74_00570"
     CDS             complement(11207..11530)
                     /locus_tag="BEN74_RS00575"
                     /old_locus_tag="BEN74_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004935749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_068912089.1"
                     /translation="MKVFIITSKVHALQKSIMPVSSKAYVNCYTGGNDYEEAIKKVFER
                     FLIDGIHPDEILNPICEMPLEDWTIHIKEKYGDLAQRMLSQKDFETRILSNEVVYGGFA
                     CFD"
                     /name="hypothetical protein"
     gene            complement(11604..12002)
                     /locus_tag="BEN74_RS00580"
                     /old_locus_tag="BEN74_00575"
     CDS             complement(11604..12002)
                     /locus_tag="BEN74_RS00580"
                     /old_locus_tag="BEN74_00575"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_068912087.1"
                     /translation="MKRKKSVRFFLLLPIIDQLINEGYTQQSIVEKLQMDHELDLNLNT
                     FKSYLYRYRSQDKKTQEESYSNKPKTETSSGFLSSFQDAEKKENDLEESKAISLADRQG
                     MKGDISRSRAKAKELLDSLSLSKLKIDQ"
                     /name="hypothetical protein"
     Typing          2..954
                     /name="rep_cluster_1172"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="87.552"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atgagagaat tagttgtaaa agacaatgcc ttaatcaatg caagctataa tttggattta
       61 gtagaacaac gtttaatctt attggcgatt gttgaagcaa gagaaagtgg caaagggatt
      121 aatgctaatg atccccttga agtacatgca gaaggttata tcaatcaatt tggcgtacac
      181 cgcaatactg cttatcaagc cttaaaagat gcctgtaatg atttattcgc aagacaattc
      241 agttatcaga aaataaatga aagagggaat attgagaatt atagatcccg ttgggttagt
      301 gaaattggat atgtagataa tgaagccgtg gttaagctta tttttgcccc agcaatagtc
      361 ccattaatta cacgcttaga agaacacttc actaaatacg aattacagca agttagtaat
      421 ctcagcagtg cttatgctgt gcgtctatat gagctattga ttgcatggag aagtatcggc
      481 tctactccag ttatagagct aagtgatttc cgacaacgaa ttggtgtgct tgatacagag
      541 tacaagcgca tggagcgctt taaaacgagt gtactagagc ttgctcttaa acaaattaac
      601 gaacatacag atatcactgt aaagtatgaa cagcataaaa aagggcgctc aatttacgga
      661 ttttcattca catttaaaca aaagaaagtg gatgctccgt ctgtaggaaa taaagaccca
      721 aacacaatag atctattttc aaaattatca gactctcaac gctatttgtt tgctaacaaa
      781 ctttctgaac tccctgaaat gggaaaatat tcccagggca cagaaagcta tcagcaattt
      841 tccattcgca ttgccgaaat gctgcaagat cccgaacgct ttaaagaact atacccatac
      901 ttaaaaaaag tgggatatat gccatccaat aaaaaggaca cagtaaatgg ctaagttatc
      961 gttaagtgaa gtatctaaac aatttcacgt tgatagatcg accatttaca gagctgtacg
     1021 caatggacgc ttatcacgct ccagtaatgg ccagtttgat ctttctgaag tcattcgatg
     1081 ctttggagaa ccagcacaag catctcagca aaaagttaca cctaagcaag aaagtgatgc
     1141 ctccacacaa aaactcatca ctcatttaga gaatgaagtt aaaaaatacc aggagcgcga
     1201 agaacgtttg atgcagcaaa ttgaccgcat gcaaaccctc attgagttaa aaagtgttgc
     1261 acctgctaca gcagtgccac aacaagatgc cacggcatgc gacacatcaa tgccacggca
     1321 tgcgacaagt aatcaaataa atgaaaataa agaatattta tataaaaata tgacagaaaa
     1381 tgtggcagtg ccacaacaag atgccacggc atgcgacaca tcaacgccac agcatgccac
     1441 gctgcaaaat gtggcaatgc cacagtctaa aaaacgcggt ttatttggac gggtattcag
     1501 cgcagtcttt aacgaaaatt gagccttcca agaaggagat ccagcgtgaa aaaactgaaa
     1561 gaagttttac aaggcattgc ctttctgctt gccggtattc ccatcatgat cattgccagc
     1621 atcatggatc aaaaagcctt caaaaaagaa cgggaacagc gccagaaagc ccaagagcaa
     1681 agcaagtgat tgtgcagtga gatttatcaa tccaggctca aattgctgat aaaataggca
     1741 tattggatga atgatccact aaaaagcccg gtttgtgcaa accgggcttt tcagcggatc
     1801 tcaagatctt gatttaatta aaattattga gagcatttcg cataacgtgc attatgttaa
     1861 ttttagaaaa actaaacttt taagtagatg agtactaaat ttaatattta atttagtatc
     1921 attctcttga cataagcaat ctacattctt tacattagtt ctaacgacgc ctgtaatgga
     1981 gatcataata attttatgat atacattatg ggcttttcta tttttattaa ctagatgaat
     2041 actttacaag aatattgcaa accatatata tcaccccaaa aattagtttt agattattta
     2101 atatctaaaa aagggctagc tattcaagat aatgaaattg aatttgctga acaggctcta
     2161 tctcaaatta attggtatca tctaaaaatt tacttctacc cattcattga ggatttaact
     2221 gctgaggaag aacgatataa acctcaaaca aattttcgta atggatggga catttattta
     2281 ttagatgatg agttaagaaa aattgtaatt aagcacacac taaaaataga gttgattgtt
     2341 aaaagccata tagatcaggc aattactgag tttacagaag atcctttttg gtacctcaat
     2401 gatgattact ttataaaaaa taaacagcct tattatgaaa gaaacaaggt tctgggtagt
     2461 atgaatagct cagatgcaga gtttacaaaa catttccaaa aaaactataa aagtccttat
     2521 aaatcatata gatcacttcc tccattttgg atagctatgg aagtaattac ttttgatcag
     2581 tttttaaaaa ttatagaaaa agtgaatccc caaatttttg aaaaaagggg agaaaatgct
     2641 ttagacaaat gtgcaatgaa attaggtgca gaaaatttca aacagctaaa aagttggcta
     2701 gaagttataa aagatatccg aaataaaggg tgtcataata gtcgattatg gaacgctaat
     2761 catatgatcc cgaagggact tgatatacat tcacagccaa tagaaagttt gtctaaacct
     2821 cataaaatat atttagttac gttagccata tttttaatga ctaaaaattc tgttgcgatt
     2881 gataagaata ttaaagaaga attagaagag ctttttgaag cttatggaag caagatcaat
     2941 gatctagaga aacaaatggg cttccctgaa agttgggcta caaaggctat atggacttaa
     3001 tgaaccagaa tctctcccca tataccatct gttatcgttt cgggtatgag cgtttgtgtt
     3061 ttaccacatc ttcccatgca cctagacgat agcgacgata tgatcggacg ttgactaatt
     3121 tctcagacat tgtctatctc ctatctaata aagtcagttt tgggaactga cacccatgct
     3181 tagctaaaaa tatgcttata caatgggtaa gaattggaga gattccttga agggaattat
     3241 atctaactat ctattttttt aaagttaata ttctactttt tttaaatgat ctttagatgc
     3301 ttttaactct ttaatctttt ttagcctttc ctttctttta tgggcagcta tataccactc
     3361 atcaaataac atttctaata ggcttattag aagttgagct tcatctggtt caacatcaac
     3421 aataatattg atatcttttt ccatatgagc accaatattt cctagtgatc gaatagaatc
     3481 aatagcttcc caaacttctg gttctatttt attttcaata gcttttattt cttcgaatag
     3541 atttttttct tttacattcc aaaaatccct tatcattcct tgaaggcatc ttctggctaa
     3601 tgttgcagag gcctttggac ttaggtcctt aattaaacaa gcttcttcat aatcattaat
     3661 aattacttct ggaatgatgg ctgttgaata tgtttttgaa ctgctttgtg gtaatagatt
     3721 ccaggttttt tctggctttt cttcgggggt ataatcgtat gtagtagcac ttttaaagag
     3781 tagcgctttc gctgatattt ctttgcattc aggattcgga cagacaacaa ttattgtatt
     3841 cacccctaca agacgatcat atttattatt gagcctaaat ctatgagtac tatcactata
     3901 attttcgcta gttactgtac aagtatgatt acaataagga catttccaag gaaaagaaga
     3961 catattactt cctataaatt gaattagatt ttactatgaa tatttgaata atataagttt
     4021 atacagttat ttaacataaa atctcttatg cgaaattcga ctgtaagccc aaagtagaaa
     4081 tgtcctagat aaagtgagct gattaatcat tttattcggc tcacaatatg agccgaatag
     4141 atgctatatt taactcatct gaaattttag atgagcgatt gaaaatgcag gaatggatct
     4201 ggcaagctga aaactggaca aattttacat ggctagattc aattatcttg cctaaaacca
     4261 gacagataca tcaaaaaata gggattcttc ttgggcaaag tcagcatgat ttagcaaaag
     4321 aacagttcac cctagatact ctgcttgcca atcttgttgc ctcatctgca attgaaaacg
     4381 aaactatcaa tgttttttca ttgcgatcat ctctagctca acgtttaggt gtcacacttg
     4441 aacagcctta tcccagctca gatcgatcag aaggtttagc taatatcatg ctagatgcgc
     4501 ttaatgatgt taagcaacca ctgactgttg agcgtctcat gcaatggcat cgttggcttt
     4561 tcaatgaccc agattggaca atgcaacgtt tacgcattgg tcaacttcga ggatcagagc
     4621 ctatgcaggt cgtttcaggt cgattggatt accctaaagt acattttgaa gcaccgccaa
     4681 gagaaggctt agaagaacgc ttaaacacct tcatagcgtg gtttaatcag agtctgaatg
     4741 attcattgct tgatcctcta ttacgtgcag gcattaccca tttatggttt gttaccctac
     4801 atccttttga tgatggtaat ggacgtatta cacgtacact tacagacctt gctttagctc
     4861 aaatggatga acaaagcatc cgtttatatg caatgtctac tgcgatattg aataaacgta
     4921 aaagttacta tgaaatatta gaacaaaccc aaaaaaatga ttctgatatc acagattggc
     4981 tagtttggtt tttagataca ttgaatgata gccttgaaaa aaccttagca cagatcaatc
     5041 gaatattatt taaaagccag ttttggcata aatattcaaa tttagccctg agtgaagaac
     5101 aacgcaaagt cctaaatcgt ttattagacg gtggagaaaa tggctttgaa catggtatta
     5161 gtgcttcaca atatcaaaag gttgctaaaa caagtaaggc gacagctact cgtcatttat
     5221 ccgacttact tgagaaagaa tgtatcgtca aattggaagg tggtggacgt aatacacgct
     5281 accagatcaa tactcagtta tagatattta agcaataaaa aaccccggca tcctgccggg
     5341 gtttttcgta ttggttcgct cggtataact cctgtcgtac cttacagtcc ttgtgcgtat
     5401 cgttgttctt attattgtct ggaacttcct gttctccatg tccccatgat atgaaattgc
     5461 agccaggccg cataggcgca aagggccgta attgctgtga gcatatgcgt acaaggccgg
     5521 ctaaaaactg tcctcttttt atccgcagaa attggggata attttctccc tgggaaggac
     5581 gggaacactg aaaaaagttc caacttgatt aaaaaataat caaatttatt ggcaataact
     5641 atcaaaactg ttcataaaaa agccgactta tttcatagcc ggatttttgc tatctttcag
     5701 gtcggttaaa aatttttaac ttgttgaaaa acaaaatctt tttgtattgc gtttcgtata
     5761 acgcccatta tgttaaatag aatgtaaatt aagctaaaat tatgttttta atgtagacaa
     5821 accaagagct ggaataggtg atagttgcgt cgatcgatga tcgttatatt gatatcagcg
     5881 ccggataagc cattgacagc gtctaaccca ccgcagcctg cgccgacaac aacatgatgc
     5941 cgtttgtcag aggccatttt tattatcagg gtctcctaat gttcagcgat tcaatgcggc
     6001 cgcatggtgg gccatgtgtt cgctcaagta ggtcgaaata aagtagtagc tatggtcatg
     6061 accaggccgc agattaagcg tcagcgggtg gcccacctgt tcacacgctg tacgcaacag
     6121 ttgcggtcga agctggtgct caagaaattc atcgctcagt ccttgatcga caagcagcgg
     6181 taagcgttct tgcgccacgt ggataagctc gaccgtgtca tattgcttcc atgcgtccag
     6241 atttttttcg cccaagtaag ccgccagggc cttctgtccc catggaactt gcgtaggcgc
     6301 cacgataggc gagaacgccg agacacttgc gtagcgaccg gggttgcgaa gagcaataac
     6361 cagagcgcca tgaccgccca tggagtgccc actgatgctg cgtcttgacg acgcggggaa
     6421 gttcgcttcg atcagcgctg gtaattcgtc gaccacatag gaatacatct gatagtgatc
     6481 gctccaaggt agctctgtgg cgtctacata gaaacccgca ccttgaccga gatcataggc
     6541 agcatcgtct gcaacgcttt ctccgcgcgg gctggtatct ggggctacga caatgattcc
     6601 gtgctccgct gcataccgct gcaccgaagc cttagtgatg aagttctgtt cggtacaggt
     6661 cagacctgag agccagtaca gaactgggag cttttcatgc tcagcctgag gcggaagata
     6721 gattccaaac ctcatcatgc agccaagcac tgttgactca tgctgaaaaa cgtcctgcca
     6781 accctcgaag ctggcgtgat gttcaatgcg ttccacggat tttctcctta tttcaacgag
     6841 gtggtgctca ggcgccaatc aagacgactt ttcgtcccat tcaggattag ttaaaaacgc
     6901 ttcaccgcac cactcatcag gtttccagcg ttgtcgctgg catgaggatc gctgctacaa
     6961 acccagacat caccacacca cgaaggtcgg atgtcggctt caatgtacaa gcaataaagt
     7021 gtgtaattca gggaggcact accgcgatgc ctatttcgaa ttctgtgttt cttgcatcac
     7081 gcaccgaact tttagtccga tcgtgtttat tgaccgtctt tggccatgaa ggcgaggcaa
     7141 catctttctg agctctaatc agcagataga tattcagagt actataaacc gtgtggaatg
     7201 agcgggcatg ccacacggtt gcggtggaag atgaaatcgt tttggctgga atcagccttt
     7261 tcacaataac ctcacttccc ctagttttag acaaaggtgt aggcgcagac cttattacca
     7321 gcaggatcac gcaggtaggc cgcgtaagca cccggcaggt ggccacgtgg gccaggctga
     7381 ccttcatcgg tgccaccagc ggcgaggccg gctgcgtgga atgcattcac ctctgcagga
     7441 gtggcagctg cgaagcctac cgtcacacca ttgctggttg gttctgcacc attgcctggg
     7501 cgggcgatca tgaaagcagg tttgtcgcga ccgtacagaa cccaatcgtt accaaatggt
     7561 cctaggtttt taatgccgag ggcacctaaa gtggcatcgt agaatgcaac cgacttatta
     7621 aggtcgactg cgccgaggaa gacatgggag aaaatgtcgt taccggaaat tactggaata
     7681 gccattaaag tgtttccttg atggatggtt ctgaaaaatc aggtagtgat ccatggatca
     7741 atagtgaatg acagtacgaa tcgacttgcc ttcgtgcatc aggtcgaacg cgtcattgat
     7801 ttcatcgagt cccatggtgt gggtgacgaa tggagcgagg tcgatttcac ctttcatcgc
     7861 gtcttctacc atgcctggaa gttgggtacg tcccttgaca ccgccgaagg cagagccctt
     7921 ccaggtccgg ccagtgacta attggaacgg acgggtagag atttccttac cggcgccagc
     7981 aacaccgatg atgatcgatt gaccccagcc tcggtgggct gcttccagtg ccgaacgcat
     8041 cacattgacg ttgccaatgc actcaaaggt atggtcgaca ccccagccag tcatttcgat
     8101 aagaacctcg tgaatcggct tctcatagtc tttggggttg atgcactcgg tggcaccaaa
     8161 actgcgtgcc agttcgaact tggatgggtt agtatcgatg gcgatgatgc ggccagcctt
     8221 ggcttggcgt gcaccctgaa tcgcagcaag accgataccg ccgaggccga acactgccac
     8281 ggagtcccca ggctgcacct tagccgtgtt gtgcacggca ccgataccgg tggtcacgcc
     8341 acaaccgagt aggcagacat gttccggatt ggcgtctggg ttgatcttag ccagcgatac
     8401 ctcggcaacg acggtgtatt cgctgaaggt cgagcaaccc atgtagtgga acagtggctg
     8461 accgttgtat gagaaacggg tagtgccgtc aggcatcagg cccttgccct gagtagcgcg
     8521 cactgccaca caaaggttgg tttttcctga cttgcaaaac aggcactcgc cacattcggc
     8581 ggtgtatagt ggaatgacat gatcgccagg tttcacacta gttacacctt caccgacctc
     8641 gacgacgatg cctgctcctt catggcccaa cacgactggg aagatgcctt ctggatcatc
     8701 gccagaaagg gtgaatgcat cggtatggca aacgccagtg tgggtgatct tgaccagcac
     8761 ctcacccttg cgcggcggct cgacgtcaat ttcaactatt tccagaggtt ttcctggccc
     8821 gaaggccaca gctgcacgtg atttcatgga atctttcctt acacaaattt attttaaata
     8881 tgacttaaga atctttgcta tttcagcaag ttcttcgttt cgttgttgtt ctgtagtttc
     8941 tccagatact agagtgtctt ttaagtgaac ctctagcatc tcattcatta gaccattaat
     9001 ggcaccgcgt acagaacaga tttgttgcag aatggcatta cattccacat tgctttctag
     9061 cgctttttca atcgcttcag cctgaccctt gattcgtctg acacgcgtca gaatcttttt
     9121 tttatcttcg acttgatttg gcataaagct aattccttaa agtttcttat agtatagtac
     9181 cctatagtat atggaatgac aagaaacctt tataaactta ataattgata aaagctttga
     9241 atagccacca aaactctaga cacacataac catcttttga tgggactttt cataatctaa
     9301 tggagaacgt tgtccattgg aaccatgtct tctttttgag ttataaaaca tttcaatata
     9361 ttcaaagata tctgacctcg cttcagttct tgatgcatag tttctcttct taactcgttc
     9421 ccgttttaat agctggaaaa agctttctgc aacagcatta tcatggcaat tgcctctacg
     9481 actcatactg cattccaaat tatgttgttt aaggaatgct tgccattcat gactggtata
     9541 ttggctgcct tggtcagaat gaatcaaaac cttgttcttt ggttttctcc gccataaagc
     9601 catcaataac gcatctagca caagatctgt agtcattctt gatttcatag accacccaac
     9661 aacaaggcgt gaaaatagat caattacaac agcaagatat aaccaacctt catgtgtacg
     9721 aatatacgta atgtctgtta cccacttccg attaggctga gttggattaa actgtcgttc
     9781 taaggtgttt gcggaaacaa tacttggcgt acctgcataa gatctaggct tgcgatagcc
     9841 gcgctgtgat ttaagcccat tcgctttcat taacctatgc acccggttga taccgcaatt
     9901 ttcgccaaca tctttcaaat cacagtgaat cttgcgatag ccgtagactc cgccagattc
     9961 cagccagaac tgtttaatca atcctgaaag ctgttgtcgt ttcctcgcag ttttactagt
    10021 gggttgtttc aaccatgcgt aataaccact gtgatgaaca tctagagtcg aacataaacg
    10081 acgaacagac catatgtgct gattgtcctg aataaaggca tacctcattt ggactggctt
    10141 gcgaagtaca ccgcggcttt ttttaatatg tccctttctt cagtaactct ttgcaactcc
    10201 ttttttagct ttgccaattc tgaaactgca tccctagagt ctgtgatttt aggttcttga
    10261 ggagcataac gcttgatcca agcataaaga ctatgcgtgg ttgtacctaa acgtgcggca
    10321 acttcagcca cgctatgacc tttttcagtc acttgcttta ctgcttcaat tttgaattct
    10381 tcagggtatc gtttgctact gctcataagc acctcgttaa ttagccattt tatctaacta
    10441 aaaggtgtct acaaaatcgg tggctattca cttataatta aaaaattcta catctatttt
    10501 taaatttttc gaaaattaac ataatacgcg ttatacgaat ctaagaatgg gagcctttag
    10561 ctcccatttt tactatattt tttaaagttt taggcgtgtt gtactgtgag ttttaggcct
    10621 aaagcattca ctacacgatt aatcgtatca aaacgtggtg ctgaatcatg acgtagggct
    10681 ttgtagagtg cctcacgccc aattcctgct tctttagcga tttgagtcat accacgagct
    10741 ttagcaataa caccaagtgc atgagctaat tcagctggat cattctcttc aagaaccata
    10801 ttgagataca tcacaatgtc ttcttcagtt ttgagtgact cagccatatc aaaacttggt
    10861 aaatcagcta ctttaaccac tttaatcctc caacgttttc gatagtttga ctgcagaatt
    10921 caacgtttta tgtcctcata gaattaatca atatttttga aaatgatatt ctttttaaat
    10981 gggaagtcta taatgataga taaatgcaat ttaaattaga tagttaccaa ttttctagaa
    11041 attttatagt ttttctgaaa ctaacataat acaccttata cgaagtcaag aaattgagtc
    11101 tatatcctta attttttatt atttttttaa atttaggagc ctagattctg gtctaagctc
    11161 ttaaatttca tgtaatcgcc attatgttaa atagtgattt ttttgtttag tcaaagcaag
    11221 caaatccacc atatacaact tcattagata aaattcttgt ttcaaaatcc ttttgagaaa
    11281 gcatccgttg agctaaatca ccatatttct ctttaatatg aatagtccaa tcttcaagtg
    11341 gcatttcaca aattggattg agtatctcat ctggatgaat tccatctatc agaaatcttt
    11401 caaatacttt ttttatagct tcctcataat cattcccacc tgtatagcaa tttacatatg
    11461 ctttgcttga gacaggcatg atagattttt gtaaagcatg aactttacta gttatgataa
    11521 ataccttcat tctatttatt taaattctct aaaattaaca taatacgcgt tatgcgaaag
    11581 gcccgattca tgggcctttt ttgctactga tcaatcttca gtttagatag agacaggcta
    11641 tctaatagct ctttggcttt agcacgcgat ctacttatat cgcctttcat accttgccga
    11701 tccgctaggg atatagcttt gctttcctct aagtcattct cttttttttc agcatcttga
    11761 aaactagata agaaaccaga gctagtctct gtttttggct tattagaata agattcttct
    11821 tgagtttttt tatcctgtga gcgatatcga tataaatagc ttttaaatgt attcaaattg
    11881 agatctaact catgatccat ttgaagtttt tccacaatag attgttgcgt atacccctca
    11941 tttataagtt ggtcaatgat tggtaaaagg agaaagaacc tcacactttt tttccgtttc
    12001 atattcagtt tttctaagat ggaatatgta caatatacag attaattgcc tactttttac
    12061 ataaaatttg aaactttttg ctaaactata aaaactattt gcattatttt tgcaactatt
    12121 tactaaaatt ttgcatccta tttgcattta aattaaaaga attaaggaat aaacgcccta
    12181 aaaagctcaa aatagcgaaa aattcaccca ataattttaa gtttttattc ctataaagac
    12241 ccttatttta tggatacacc atacgcacct tgcaggatgc caaaaatcaa cggtttacaa
    12301 actttagaaa agtataaaaa ttagaaacct catacataag taagcctatt acattgattt
    12361 ttatagtatt gcgatgcatg aagtaaaaaa ctctttataa cgtggattat agtgagtgca
    12421 attcagccat actcatgctc aattcttcag ttatacaaat ctaagtttta aaaatttgag
    12481 atagcctttc tttttctgaa tgggtaaatg gctacgtctg tttttcaatt gaatgaaggc
    12541 agccataaat tgcgtttaaa tgcgttttaa tagcacttaa atagaaaaca gagaactttc
    12601 ttgtctcgta ttgaaaacaa gtctaaaaac gcaaacgaga gcaattgaag cctactttga
    12661 tttaaaccat aagaacagaa cgagtgtcta cgagtgatac cccctcaatt ggaatttttg
    12721 ccgctctttc tctgattaca agctttctat ccataaatca ccttcatagc agagcagccc
    12781 gaatgggtgc gaactattag gcatttgctt ctagaccgag cgaagcgagt aaaaaaactt
    12841 atgagcgaag cgaattccga gtttcttttg ctttttctaa gagtcacgga gatattgacc
    12901 gaaaattgcc ccggcgaatc gagcgaaagc gagattcaat agagtttgag cgaagcgaaa
    12961 accgagggca atttttcata cccagggctt ttaattattt ttaagctttt aaatgctttt
    13021 agatatgctg aaagtcttta ttaaaagcac ttacagagct tatatgacac cgtttacctt
    13081 gcaatatgac accgtttacc ttgcaatatg acaccgttta ccttgcaata tgacaccgtt
    13141 taccttgctt atagcaccaa aattaattat tgtgtcatag cataataatt aattttggtg
    13201 cttc
//
