LOCUS       NZ_CP032270            14502 bp    DNA     circular CON 05-JAN-2024
DEFINITION  Acinetobacter sp. WCHAc010034 plasmid p2_010034, complete sequence.
ACCESSION   NZ_CP032270
VERSION     NZ_CP032270.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN05356835
            Assembly: GCF_001696615.3
KEYWORDS    RefSeq.
SOURCE      Acinetobacter sp. WCHAc010034
  ORGANISM  Acinetobacter sp. WCHAc010034
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter.
REFERENCE   1  (bases 1 to 14502)
  AUTHORS   Hu,Y., Long,H., Feng,Y. and Zong,Z.
  TITLE     The complete genome of Acinetobacter sp. strain WCHAc010034
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14502)
  AUTHORS   Hu,Y., Long,H., Feng,Y. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2018) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
REFERENCE   3  (bases 1 to 14502)
  AUTHORS   Hu,Y., Feng,Y. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAR-2017) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.6
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology             :: Illumina HiSeq; MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/04/2024 21:32:37
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,697
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 112
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 21 of 68
            Pseudo Genes (incomplete)         :: 53 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 16 of 68
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP032270.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..14502
                     /organism="Acinetobacter sp. WCHAc010034"
                     /mol_type="genomic DNA"
                     /strain="WCHAc010034"
                     /isolation_source="hospital sewage"
                     /db_xref="taxon:1879049"
                     /plasmid="p2_010034"
                     /geo_loc_name="China"
                     /collection_date="2015-10-31"
     gene            1..930
                     /gene="repM"
                     /locus_tag="BEN74_RS00425"
                     /old_locus_tag="BEN74_00425"
     CDS             1..930
                     /gene="repM"
                     /locus_tag="BEN74_RS00425"
                     /old_locus_tag="BEN74_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004282204.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepM"
                     /protein_id="WP_038343842.1"
                     /translation="MKTELIVKDNALINASYNLDLVEQRLILLAILEARESGKGINAND
                     PLTVHAESYINQFGVHRNTAYQALKDACDDLFARQFSYQSLSEKGNVINHKSRWVSEVA
                     YIDNEAVVRLIFAPAIVPLITRLEEQFTKYEIQQISNLTSAYAVRLYEILIAWRSTGKT
                     PLITLYDFRQKIGVLDTEYKRMYDFKKYVLDIALKQVNEHTDITVKVEQHKTGRSITGF
                     SFSFNQKKSATQSVGSKRDSNTLDLFSTMTDKQRHLFANKLSELPEMSKYSQGTESYEQ
                     FAVRIADMLQDPERVKEFTPLLRKVGFQ"
                     /name="repM"
     gene            998..1543
                     /locus_tag="BEN74_RS00430"
                     /old_locus_tag="BEN74_00430"
     CDS             998..1543
                     /locus_tag="BEN74_RS00430"
                     /old_locus_tag="BEN74_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004918462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication DNA-binding protein"
                     /protein_id="WP_068911103.1"
                     /translation="MKKLSVSELAKLYGFSRQAIYAHINKGNLSKGADGLIDFSEALRV
                     FGEPQKKGSTVNQSQSIDSQKLTEIDLLKRQVELLEKQLNQAQLRESQSLERESFYQEQ
                     IEAMQRLLEAPKTNMTTFTDQKPSQDIATDPRLEPDQKHDGLTTPDQKENKRIPVPEHI
                     EQEPEKRGFWSRFFRPYD"
                     /name="plasmid replication DNA-binding protein"
     gene            1749..2024
                     /locus_tag="BEN74_RS00435"
                     /old_locus_tag="BEN74_00435"
     CDS             1749..2024
                     /locus_tag="BEN74_RS00435"
                     /old_locus_tag="BEN74_00435"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004967304.1"
                     /translation="MKNKIEDLKSTIQELIELFEEQNLEHWAEMYKRILFYIDDDLEYA
                     KYKIQSTIGGMGSMDDIVLYRNGDPLIKENNRLDYLRSKLYVFCES"
                     /name="hypothetical protein"
     gene            2146..2466
                     /locus_tag="BEN74_RS00440"
                     /old_locus_tag="BEN74_00440"
     CDS             2146..2466
                     /locus_tag="BEN74_RS00440"
                     /old_locus_tag="BEN74_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_000897307.1"
                     /translation="MLFIETSIFTKQIKDLVSDEEYRQLQQDLLVQPDRGDLIKNGGGI
                     RKVRCAQGNKGKSGGIRVIYYWVTEDDQIFFLVAYPKSVKDNLTDKETAILHQLVKEQF
                     HG"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            2459..2731
                     /gene="nadS"
                     /locus_tag="BEN74_RS00445"
                     /old_locus_tag="BEN74_00445"
     CDS             2459..2731
                     /gene="nadS"
                     /locus_tag="BEN74_RS00445"
                     /old_locus_tag="BEN74_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000369784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
                     /protein_id="WP_000369781.1"
                     /translation="MDNNLFDDLVASIKEAGAIKRKEVKASRVTELELPDIKEVREKTG
                     LSQNEFAARLHISPRTLQNWEQGRRYPTGPAATLIRILDAHPSLI"
                     /name="nadS"
     gene            3224..4699
                     /gene="msr(E)"
                     /locus_tag="BEN74_RS00450"
                     /old_locus_tag="BEN74_00450"
     ARG             3225..4696
                     /name="msr(E)"
                     /gene="msr(E)"
                     /gene_name="ABC-F type ribosomal protection protein Msr(E)"
                     /tool="amrfinderplus"
                     /drug_class="MACROLIDE/STREPTOGRAMIN"
                     /identity="100.0"
                     /coverage="100.0"
     gene            4755..5639
                     /locus_tag="BEN74_RS00455"
                     /old_locus_tag="BEN74_00455"
     CDS             4755..5639
                     /locus_tag="BEN74_RS00455"
                     /old_locus_tag="BEN74_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mph(E) family macrolide 2'-phosphotransferase"
                     /protein_id="WP_000155092.1"
                     /translation="MTIQDIQSLAEAHGLLLTDKMNFNEMGIDFKVVFALDTKGQQWLL
                     RIPRRDGMREQIKKEKRILELVKKHLSVEVPDWRISSTELVAYPILKDNPVLNLDAETY
                     EIIWNMDKDSPKYITSLAKTLFEIHSIPEKEVRENDLKIMKPSDLRPEIANNLQLVKSE
                     IGISEQLETRYRKWLDNDVLWADFTQFIHGDLYAGHVLASKDGAVSGVIDWSTAHIDDP
                     AIDFAGHVTLFGEESLKTLIIEYEKLGGKVWNKLYEQTLERAAASPLMYGLFALETQNE
                     SLIVGAKAQLGVI"
                     /name="Mph(E) family macrolide 2'-phosphotransferase"
     gene            complement(5829..6440)
                     /locus_tag="BEN74_RS00460"
                     /old_locus_tag="BEN74_00460"
     CDS             complement(5829..6440)
                     /locus_tag="BEN74_RS00460"
                     /old_locus_tag="BEN74_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004889724.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_015060246.1"
                     /translation="MIEMNTRIYLRASTKDQDAERALQILQDLNQNLNLGETIVYVENY
                     SGTKLDRPELNKLLSEANQGDTLLVESIDRLSRLTQRDFQELKRRIQEKGLRLVVADLP
                     TTYQMIQTSDSITHSILELINNMLIDLLATMARLDNEKRIERIKQGLARSGYKPTGKKA
                     NEAKHKRIKELLVVGNMTKEEIAKAVNCGVATVYRVAKVI"
                     /name="recombinase family protein"
     gene            6657..7517
                     /gene="aac(3)-IId"
                     /locus_tag="BEN74_RS00465"
                     /old_locus_tag="BEN74_00465"
     ARG             6658..7514
                     /name="aac(3)-IId"
                     /gene="aac(3)-IId"
                     /gene_name="aminoglycoside N-acetyltransferase AAC(3)-IId"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            7530..8072
                     /locus_tag="BEN74_RS00470"
                     /old_locus_tag="BEN74_00470"
     CDS             7530..8072
                     /locus_tag="BEN74_RS00470"
                     /old_locus_tag="BEN74_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="WP_000587837.1"
                     /translation="MIIWINGPFGAGKTTLAKRLRDRRSKSLIFDPEEIGFVVKETVPM
                     PASGDYQDLPLWRGLTIAAVREIRRNYSQDIIIPMTLVHPDYLTEILDGVRRIDDQLLH
                     IFLTLNEDLLRHRIANQTMHPDPNRNAEIREWRLANVARCLAARERLPCTTRVLDSGAH
                     TSDELAAMVLDGIDGRT"
                     /name="AAA family ATPase"
     gene            complement(8554..8745)
                     /locus_tag="BEN74_RS00475"
                     /old_locus_tag="BEN74_00475"
     CDS             complement(8554..8745)
                     /locus_tag="BEN74_RS00475"
                     /old_locus_tag="BEN74_00475"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000951934.1"
                     /translation="MAIALMGAGFSATDTSDAVNILYPITMTVQANKAWQASGLKKSFF
                     LPSHKKVRGGEKISYARL"
                     /name="hypothetical protein"
     gene            <8862..11228
                     /locus_tag="BEN74_RS00480"
                     /old_locus_tag="BEN74_00480"
                     /pseudo=""
     CDS             <8862..11228
                     /locus_tag="BEN74_RS00480"
                     /old_locus_tag="BEN74_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014702729.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element ISKox2 family transposase"
                     /name="Tn3-like element ISKox2 family transposase"
     gene            complement(11587..12018)
                     /locus_tag="BEN74_RS00485"
                     /old_locus_tag="BEN74_00485"
     CDS             complement(11587..12018)
                     /locus_tag="BEN74_RS00485"
                     /old_locus_tag="BEN74_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004986359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_039090891.1"
                     /translation="MEDPTLNKLIRNTELFFEHHWNVDLLGKPPEWSPVHFNFGKIPNY
                     DKQGVYAFVKGDLVTYIGVGTSRGAGRYRGNGLSARVMKYCRWNEDKTEYIAIDPRLKD
                     AGSIITIGFERDTAYLANALEVFLISKIQPVHNVVKVGM"
                     /name="hypothetical protein"
     gene            complement(12036..12302)
                     /locus_tag="BEN74_RS00490"
                     /old_locus_tag="BEN74_00490"
     CDS             complement(12036..12302)
                     /locus_tag="BEN74_RS00490"
                     /old_locus_tag="BEN74_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005268842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="WP_088749624.1"
                     /translation="MSVTETLDSKIKAQEEKLKQLKAQRQAALARERAKEKEQARKDDT
                     RRKILIGSCMLKITEDDEQARAKLIAQMDKYLTDERDRKLFNL"
                     /name="mobilization protein"
     gene            12512..13948
                     /gene="mobQ"
                     /locus_tag="BEN74_RS00495"
                     /old_locus_tag="BEN74_00495"
     CDS             12512..13948
                     /gene="mobQ"
                     /locus_tag="BEN74_RS00495"
                     /old_locus_tag="BEN74_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005248576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_068911104.1"
                     /translation="MAIYHFSVKTVARSAGRSATAAIAYRAGEKIYCEREGREHDYSRK
                     TGVEYKEIYLPEGAPEHLKNREKLWNEVEQRETRKNSTVAREFEIAFPSELNQEQRLAM
                     LEELCASIVERHQIAVDACIHAPHTGSGSDERNYHAHILMSTRKLTPEGFTEKTRELDQ
                     KHSGEIEHWREHFADICNMHLDMAGSTARVDHRSYKDQENGLEATLHEGPKVTELRRRG
                     IETEISRTNDEIKERNQAQLQYGKNMDLLIAENEITLKKLKTEQQMHIQNSAKTPPLSA
                     EEQFQYIQRENLSKLLKGEISAKEANLDLDFMQHNLEKVEGILSKQQKHKNDFNQQLAQ
                     DIVKSRLKQSHDKLQSLVDQHNELGQNKPLLFGKKAWEAQRDAIYQEHKQLKDRHEYQK
                     KNGVKDLLENEKFKAHAWTQYQKEHPAKAEQYQNLYQSYRAIKNGVDKIKAEQQMKLRQ
                     EQQLKAQQQAPKMKSRGMRR"
                     /name="mobQ"
     ARG             4756..5636
                     /name="mph(E)"
                     /gene="mph(E)"
                     /gene_name="Mph(E) family macrolide 2'-phosphotransferase"
                     /tool="amrfinderplus"
                     /drug_class="MACROLIDE"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             3225..4699
                     /name="msrE"
                     /gene="msrE"
                     /gene_name="msr-type ABC-F protein"
                     /tool="rgi"
                     /drug_class="macrolide antibiotic; streptogramin
                     antibiotic"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             4756..5639
                     /name="mphE"
                     /gene="mphE"
                     /gene_name="macrolide phosphotransferase (MPH)"
                     /tool="rgi"
                     /drug_class="macrolide antibiotic"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          2..930
                     /name="rep_cluster_1254"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          12513..13945
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atgaaaacag aattaatagt taaagataat gccttaatta atgccagtta taaccttgat
       61 ctagtggagc aacggttaat tcttctagcg attcttgaag caagggagtc gggtaaagga
      121 ataaatgcta atgatcctct aacagttcat gctgaaagtt atatcaatca atttggtgtt
      181 catcgaaata cggcttatca agcattaaaa gatgcttgtg atgatctatt cgcaagacaa
      241 tttagttatc aaagccttag tgaaaaagga aatgttatta atcacaaatc aagatgggtg
      301 agtgaggttg cttatattga taatgaggct gttgttagac ttatctttgc tcccgctatt
      361 gtgcctttaa ttactagact agaagaacaa tttacaaagt atgaaataca acaaataagc
      421 aatttaacaa gtgcttatgc cgttcgttta tatgaaatat taattgcatg gcgtagtacc
      481 ggaaaaacgc ctctcataac cctgtacgac ttcagacaaa aaataggggt actcgatact
      541 gaatacaaac gaatgtatga ttttaaaaaa tatgtcttag acattgcatt aaaacaagtc
      601 aatgaacata ccgatattac tgtcaaagtt gaacagcata agacaggcag atcaattaca
      661 ggcttttcat ttagctttaa tcaaaaaaag tcggctactc aatctgtcgg atctaaaagg
      721 gattcaaata cattagacct cttttcaaca atgacagata aacaacgtca cctattcgcc
      781 aataagctct ccgaacttcc tgagatgagt aaatattcac aaggtacaga aagctatgaa
      841 caattcgctg tacgtatagc tgatatgttg caagatcctg agagagtaaa agaattcact
      901 ccattactta gaaaagttgg ctttcaatga atagtgtcaa ttgacaatgt aaagttgaca
      961 gtgaagtatt aacacaaatt gacaactcat ttacaccatg aaaaaactat ccgtttcaga
     1021 gttagcaaaa ctatatgggt tttccagaca agcaatatat gctcatataa ataaaggaaa
     1081 tttatcaaag ggagcagatg gcttaattga tttctcagaa gctttaagag tatttggcga
     1141 accacaaaag aaagggagta ctgtcaatca aagtcaatca attgacagcc aaaagttaac
     1201 agaaattgat ttactcaaac gtcaagttga attgcttgag aagcaattaa atcaagcaca
     1261 acttagagaa agccagtctt tagaacgaga atctttctat caagagcaaa ttgaggcaat
     1321 gcaacgtcta ttagaagctc caaagactaa tatgactacg tttaccgatc agaaacctag
     1381 ccaagatata gcaacggatc ctcggttaga acctgaccag aaacatgacg gattgactac
     1441 tccagatcaa aaagaaaata agcggattcc agtaccggag cacattgaac aggaaccgga
     1501 aaaaagaggc ttttggagcc gtttttttag accctatgat taaccacgat tgtttaactg
     1561 atatgaacaa agtcgtgaaa cattgcttaa ataataggcg tggaacgtga aaaatcataa
     1621 aaaaaaaagc ccaccttggg ggaggttggg caaaaaagaa actacagcct tgaattataa
     1681 ggataattat aacgcatttc gtataaggtg tattatgtta attttagaga atcttaaaaa
     1741 atacttttat gaaaaataaa attgaagatt taaaatcaac aattcaagaa ctaatagaat
     1801 tgtttgaaga acagaattta gaacattggg cagaaatgta taaaagaata ctgttctata
     1861 ttgatgatga tctagaatac gcaaaatata aaatacaaag taccattgga ggaatgggat
     1921 caatggatga tatcgtttta tatcgcaatg gtgatccact tatcaaagaa aacaatcgtt
     1981 tggattactt aagaagtaaa ttatatgttt tttgcgaaag ctaaattatt cttaatatcg
     2041 ctaatttaac ataatggctg ttatgcgaaa cgagatctac cagctctcgt tttttgttat
     2101 ggaaactgct aaaattcaaa tacgccattg acgtataaag agtccatgtt atttattgaa
     2161 accagcatct ttaccaagca aattaaagac cttgtatctg atgaggaata tcgtcaactt
     2221 cagcaagatc ttttggtaca gcctgataga ggtgacctaa tcaagaatgg cggtggtatt
     2281 cgcaaagtac gttgtgctca aggtaacaaa gggaaaagtg gtgggatacg ggtgatttat
     2341 tattgggtca ccgaggatga tcagatcttt ttcttagtgg cttatccaaa atcagtaaaa
     2401 gataatttaa cagataagga aaccgccatt ctgcaccaac tcgtgaagga gcaatttcat
     2461 ggataacaac ttatttgatg acttggttgc ttcaatcaaa gaagctggag ctatcaaacg
     2521 taaagaagtt aaagcaagcc gagttacaga actcgaattg cctgatatta aagaagtacg
     2581 tgaaaaaact ggcttgagcc aaaatgaatt tgctgcacgt ctacatatta gcccccgtac
     2641 cctgcaaaat tgggaacagg gacgacgcta tccaactgga ccagcagcta cattgattcg
     2701 aattttagat gctcacccaa gccttatttg aaaaaattac ttaaaaaatg ggagcttaag
     2761 gctcccattt ttatttcgta taaggtgtat tatgttaatt ttagaaaatc ttcactacta
     2821 ttattttaaa ataagcctta tatccaagca taaaacaagg ttgtctagac ttcttttaac
     2881 agtaaagtta tcataaaact gaattttatt ttttaggtaa gtttatgcat tctatccgca
     2941 ttcgttaaga cacaactatt tgcatagtga cactatttta taatggtggg cttttgttgt
     3001 gtctttaaga atatatgcgg atatataaag taaaagtatg cttaatttat aagtatgctt
     3061 ttagtgcata gtttccagtt ataacttaat tgactagcta tttgtccacc ctgtggatga
     3121 atagcttttt ttttgggagg acactgtgat gctagctttt gttttcacct aaatcctgtt
     3181 tgctgcataa aaaatttcaa gagctaaaca ggagtaaata aaaatgagtt taattattaa
     3241 agcgagaaac atacgcttgg attatgctgg gcgtgatgtt ttggatattg atgaattgga
     3301 aattcactct tatgaccgta ttggtcttgt gggtgataac ggagcaggaa agagtagttt
     3361 actcaaagta cttaatggcg aaattgtttt agccgaagcg acattacagc gttttggtga
     3421 ttttgcacat atcagccaac tgggcggaat cgaaatagaa acggtcgaag accgggcaat
     3481 gttatctcgc cttggtgttt ccaatgtaca aaacgacaca atgagtggcg gagaggaaac
     3541 tcgtgcaaaa attgctgccg cattttccca acaagtacat ggcattctag cggatgaacc
     3601 aaccagccac cttgatctca atggaataga tctacttatt ggtcaactta aagcatttga
     3661 tggagcatta cttgttatca gtcatgaccg atattttctt gatatggttg tagacaagat
     3721 atgggagtta aaagacggta aaattacgga atattggggt ggttactcgg attacttgcg
     3781 tcaaaaagaa gaagagcgac aacaccaagc cgtagaatat gagctgatga tgaaggaacg
     3841 ggagcgatta gaatctgctg tgcaagaaaa acgccagcaa gctaatcgat tagacaataa
     3901 gaaaaaagga gaaaaatcca aaaactctac cgaaagtgct ggacgacttg ggcatgcaaa
     3961 aatgactggc accaagcaaa gaaaactgta tcaggcagct aagagtatgg aaaagcgttt
     4021 ggctgcatta gaagatattc aagcaccaga gcatttgcgt tctattcgtt ttcgtcaaag
     4081 ttcagcccta gaactgcaca ataagttccc gattacggca gatggtctga gcttaaaatt
     4141 tggtagccgt actatctttg atgacgctaa ctttataata ccgcttggcg ctaaagtcgc
     4201 tataactgga tcgaatggaa cagggaaaac gtccttgtta aaaatgatat cagaacgtgc
     4261 tgatggatta accatatctc caaaagctga aattggctac tttacacaaa caggatataa
     4321 atttaacacg cataaatctg tgctctcctt tatgcaggaa gagtgcgagt acacagttgc
     4381 ggaaattcgt gcagtattgg cttcaatggg gatcggagcg aatgatattc aaaaaaactt
     4441 atccgactta tcgggaggtg aaatcatcaa actgctttta tccaaaatgc ttttaggaaa
     4501 atataatatt ttgcttatgg atgaaccagg aaactatctt gacctaaaaa gtattgccgc
     4561 attagaaaca atgatgaagt cctatgcagg aactattatc ttcgtatctc atgacaagca
     4621 attggtcgat aatattgctg acattatcta cgagatcaaa gaccacaaaa tcatcaagac
     4681 ttttgagaga gattgttaat gatagccaat ctaatccgaa cattaattat tgaactcttt
     4741 aaaggaaatt aaaaatgaca attcaagata ttcaatcact tgctgaagca cacggcttgt
     4801 tgcttacgga caaaatgaat ttcaatgaaa tgggcattga ttttaaggtc gtttttgctc
     4861 ttgatacaaa ggggcaacaa tggttgctgc gtattcctcg tcgtgatggc atgagggaac
     4921 aaatcaagaa agaaaaacgc attttagaat tggtaaaaaa acatctttct gtagaggttc
     4981 ctgattggag aatttcatct acagaattag tggcttatcc catacttaaa gataatcctg
     5041 ttttaaattt ggatgctgaa acctatgaaa taatttggaa tatggacaaa gatagcccga
     5101 aatacataac atctttggca aaaaccttat ttgaaatcca tagtattcct gaaaaagaag
     5161 ttcgggaaaa tgatttgaaa attatgaaac cttcagattt aagacctgaa atagcaaaca
     5221 atttgcagtt agtaaaatct gaaattggta taagtgagca attggaaacc cgctacagaa
     5281 aatggttgga taatgatgtt ctatgggcag atttcaccca atttatacat ggcgatttat
     5341 atgctgggca tgtactagct tcaaaggatg gagctgtttc aggcgttatt gattggtcaa
     5401 cagcccatat agatgaccca gcgattgatt ttgctgggca tgtaactttg tttggagaag
     5461 aaagcctcaa aactctaatc atcgagtatg aaaaactagg gggtaaagtt tggaataaac
     5521 tatatgaaca gactttagaa agagcagcgg cctctccttt gatgtatggt ttatttgcct
     5581 tagaaactca aaatgaaagc cttatcgttg gagcaaaagc tcagttggga gttatataat
     5641 ttaaaaatat gattgctgag aactgccttg ttttgaaact tggttggctt taattagttt
     5701 ttagtattct ttatagaaaa tgcctcgatc aaggggcatt tctaacaatc atttaacata
     5761 aaatttctta tgtgaagtaa cctgaatgca gcggtgcccc attgggggcg cgttgagata
     5821 agcctctttt agataacttt agcaactcga tagacagttg caactccaca attcactgct
     5881 ttggcaattt cttccttagt catattgcca actactagca attcttttat tcgtttatgt
     5941 ttagcctcat ttgccttctt gcctgttggt ttgtaacccg aacgtgccag gccctgctta
     6001 atacgttcta tacgtttctc attgtctagg cgggccattg ttgccagaag atcaatcaac
     6061 atattgttga tgagttccaa gattgaatga gtaatgctgt cactggtttg aatcatttgg
     6121 taggtagtag gaagatctgc tacgactaaa cgaagtccct tctcctggat cctgcgcttc
     6181 agctcttgaa aatctcgttg ggttaggcgt gataagcggt caatactttc aactaacaat
     6241 gtgtcacctt gatttgcttc tgacagtagc ttattcaatt caggtcggtc taacttcgta
     6301 ccactatagt tttccacgta cacaatggtt tcacccaaat tcaagttttg gtttagatcc
     6361 tgaagaatct gcaaagccct ttctgcatct tgatctttag ttgaagctcg tagataaata
     6421 cgtgtattca tttctatcat ttaatcttaa ttctattaga ttaatgataa tactatcatt
     6481 taattattgc attaagtcta atgatagata ttgtatgcga tttggctcgc tatcattcag
     6541 gtatagtctt ttgataaaaa aagcatgact aaaagccatg ccttgggaat tcgtataacg
     6601 tgtattatgt taattttagg aaatcttaat cgaagtgaaa tagaggagat atcgcgatgc
     6661 atacgcggaa ggcaataacg gaggcgcttc aaaaactcgg agtccaaacc ggtgacctct
     6721 tgatggtgca tgcctcactt aaagcgattg gtccggtcga aggaggagcg gagacggtcg
     6781 ttgccgcgtt acgctccgcg gttgggccga ctggcactgt gatgggatac gcgtcgtggg
     6841 accgatcacc ctacgaggag actctgaatg gcgctcggct ggatgacgaa gcccgccgta
     6901 cctggctgcc gttcgatccc gcaacagccg ggacttaccg tgggttcggc ctgctgaatc
     6961 aatttctggt tcaagccccc ggcgcgcggc gcagcgcgca ccccgatgca tcgatggtcg
     7021 cggttggtcc gctggctgaa acgctgacgg agcctcacga actcggtcac gccttggggg
     7081 aaggatcgcc cgtcgagcgg ttcgttcgcc ttggcgggaa ggccctgctg ttgggtgcgc
     7141 cgctaaactc cgttaccgca ttgcactacg ccgaggcggt tgccgatatc cccaacaaac
     7201 ggtgggtgac gtatgagatg ccgatgcttg gaagagacgg tgaagtcgcc tggaaaacgg
     7261 catcggatta cgattcaaac ggcattctcg attgctttgc tatcgaagga aagccggatg
     7321 cggttgaaac tatagcaaat gcttacgtga agctcggtcg ccatcgagaa ggtgtcgtgg
     7381 gctttgctca gtgctacctg ttcgacgcgc aggacatcgt gacgttcggc gtcacctatc
     7441 ttgagaagca tttcggaacc actccgatcg tgcctccgca cgaggccgtc gagcgctctt
     7501 gcgagccttc aggttagagg ccgtcgacaa tgataatctg gatcaacgga cctttcggcg
     7561 ccggaaagac gacgctcgct aagcggctgc gcgatcggcg ttccaaatcg ctgatctttg
     7621 accccgagga aatcgggttc gtggtgaaag aaacggtccc catgccagcg agcggagact
     7681 atcaggatct ccccttgtgg aggggactta cgatcgcggc ggtcagggag attcgaagga
     7741 attactcgca ggacatcatc atcccaatga cgctcgtgca cccggactat ctgactgaga
     7801 tactcgacgg ggtaaggcgg atcgacgatc agctgctgca catctttctg acgctcaacg
     7861 aggacctatt gcgtcaccgg atcgcgaacc agaccatgca tcctgacccg aatcgaaatg
     7921 cggagattcg agagtggcga ttagcgaatg tcgcccgatg cttggccgca agggaacggc
     7981 ttccatgcac aacccgtgtt ctcgatagtg gtgcacacac cagcgatgaa ctcgcagcga
     8041 tggtgctcga cggaatcgat gggcgcacct gatcgccttc gacgcctgcg caaagcgtag
     8101 cgcgagggtg gcgggctcac gaccaaacgc ccagaggtcg atcatcgcag ggatgtttgg
     8161 ctttgtggtg cggacgacgg gactcgaacc cgtactctca cagagaagca gattttcgta
     8221 ccacctcgac tttcgccgcc gtctgatgac gttcgtggtc tggactgtcc cttcgccatt
     8281 gcccgaaggc tttaggcgcc gcccgtccag tctctacacc ttcccccgaa ggggcttggc
     8341 tcgggattgg cttagggtat tgcccgttag cgttccccga ctttgagcgg ttctactccg
     8401 cggatttccc cgcgggcact ccaattttaa agtctgctgc gtctaccgat ttcgccacgt
     8461 ccgccttttt tcgccgttcc tagcgctcgt gcgatgcacc tatgttgcac ctagcgccga
     8521 atcgttcttc gtcatcctga aaaaccacgt ctcctaaagc cttgcatagc ttatcttttc
     8581 tccaccacga acttttttgt gggatggtag aaaaaaagac tttttaagtc cgctggcttg
     8641 ccaggccttg ttagcttgta cggtcatggt tatcgggtaa agaatattga cggcatcgct
     8701 ggtgtcggtg gctgaaaagc cggctcccat cagggcaata gccatttcag atgcaggcgt
     8761 acagggcaat ggtcaacagc tacagcctgt ctgacgattc cggcgtcatg gctgcggcgg
     8821 ctatcacgca ttttttgttc ggtcaggcgg tgttttcgta cctctcctta agccagcttc
     8881 gagccggtgc aaaaagcgtt atggtgacag agctaaaaaa agaactgacg gtgtatcatc
     8941 agttacagcc ttatacaaga cagattgata atgctgttaa gggcttagcc ttatcgtcta
     9001 aaaaccaaca acacttcggt gaaatggtgg attattatgg cagtaaacta aaacgcttta
     9061 agcgtccaca gcaacaccta tggttgctgt gttttctgac tcaacgcata cggcaaagcc
     9121 tagagcgatt ggctgatggg tttattcatc atatccgtaa acaacaagaa gctgcgcatg
     9181 cgtttgccca acaggcagtg tttgactcat ggaggtcagc tgcagacaat gtcaccaaag
     9241 ccgcagaatt actgcatctg tttgttgatg acagtatcga cgataatcaa ccatttgcaa
     9301 cggttaggca acaagcatta agtgtaatga ataaccaaga tatagaaacg ctatgcctgt
     9361 acttaaaaaa gcaaaaacgc acggtagaag aatatcaatg gcaatattat gatgatcaaa
     9421 acgggttaat tgagcagtta ttaaggcccg tgtttctatg tcttgaatgc caagcaggta
     9481 aaggctcaga agcattagtg acccagctca aaaccacaaa aacagaacta ttagcaggta
     9541 atacattgca aacaatggat agtacactta tccaacaaaa acatcgtcca tggttaatta
     9601 acaaggatgt tgttcatcca cagcgatacg aatggttgct ttaccgtcaa ctggcttctc
     9661 ggcttaatgg tcgaatttac ttatcaaacg tcactaaata tcgtgcgcta gaagacgact
     9721 taattgcatc gtcaatacag cctgatctat tggcatcatc aacattggaa aagctaaagc
     9781 agccgattca gaagctactg caagtaaaac aaatacgctt aacaacatcc ctagaagatg
     9841 ttgctcgtca cattgataat ggtgataacc gcaatgtgat catgaagagc cgaagtggca
     9901 cacgatggcg actgccagtt aaaggctccc aatcacttgt caacaaccct ttctttaaac
     9961 aaatgatccc cgtcagtatt gctgatgtgt tgcgttatgt tgaacaagaa accaacttta
    10021 tgcaatgctt cgcacacgtg ctgccaatac aaaaacaagt aggcgccaat caggatgatt
    10081 tattggctat tctaattgcg aatgcgacac atcgcggcgt atatgggatg gcacaaatat
    10141 ctgatcgcag ttatgagcat ctaagcacta tacaagctaa ttatattagg cctgaaacct
    10201 tgcaagaggc tagtgatatc atcaataacg cagtagcggc actccctatt ttccgccact
    10261 atcacatcca agaagacgtc ctgcacgcca gtaccgatgg gcaaaaattt gaaactcacc
    10321 ttgagacttt taaaactcgc tactcctcga aatacttcgg caccaacaaa ggtattacgg
    10381 ccatgacgtt agttgccaat catagtgcgt taaatgcccg tatcatagga tctaatgagc
    10441 atgagtccca ttatatttac gacttactac aatctaatac tagtgatctt aagccagatg
    10501 tcctctcaac cgacacccat ggtgttaatc atgttaatta tgccttattg gatttatgcg
    10561 gttatagttt tgcgcctagg tatgctcagt tcaccagtgt gatcaatgac ctatttaacg
    10621 taactgaaag cgacgacgga aacacgcatc tggcgctgaa aaagccgata aaaatgaatg
    10681 tgattgaaga aggctggcag gatattcagc gtattgtgtt atcccttcaa gaaaaacgca
    10741 caacacaggc attgctagta cgcaaattat ctggttatcc atctagacac cctatattac
    10801 aagcattaac ggaatataat aggctcatca aagcccaata tttacttgat tatattgatg
    10861 atgcaagtct gcgccaatat gttcaacgag cactgaaccg aggcgaagcc tggcatttcc
    10921 tcaggcgagc tattgcatcg gtcaatcgtg accagttccg tggaaaaaac gaatcagaaa
    10981 tagtcgtctg gaacgagtgt gcccgtttga cggcaaatgc tattatctac tttaactcga
    11041 tgatcctaag tcatctatta ctgcactttg aagaagttgg tgacgaggaa aaagcagcaa
    11101 taactcgaca agtatcaccc gttgcatggc agaacattaa tctgagcggc acttatcagt
    11161 tcgctagtaa ccgcaaacta cctgatttac aggaaataac taggcctatt gttgaaaatg
    11221 aagtctaaaa tgaaaggtaa cccagctcca caagccatta actgcgaggg ttgtggcgat
    11281 ttatgttact ttagagggga aaatccctag aacccctaga agctactatt tttgatgatt
    11341 accttattga gaaaacacat tattcccatc aaattaagca aatagataat agtctaagat
    11401 atgaaattat tggatattta aaagaaggga agctagatgt taatggctta atttttgaag
    11461 atgatatttt aaatagagat tttttttact tagataacaa tacagtaaaa tggactgtcg
    11521 atagaattaa tgctgacttt gatgacagag attgaatcca tttaacataa aatttcttat
    11581 gcgaaattac ataccaactt taaccacatt gtggacaggt tggattttag agataagaaa
    11641 aacttctagg gcatttgcta aataagccgt atcacgctca aagcctattg taatgatgga
    11701 tccagcatct ttcagtcttg gatctatcgc aatatattca gttttatctt cattccatcg
    11761 gcaatatttc attaccctgg cgcttaagcc attaccacga taacggccag cacctctaga
    11821 agtcccaaca ccgatataag tcactaaatc ccctttgaca aacgcatata caccttgttt
    11881 atcgtaatta ggaatttttc caaaattaaa atgtactggt gaccattcag gaggctttcc
    11941 taataggtct acattccagt gatgttcgaa gaataattct gtattacgta ttaacttatt
    12001 aagtgttgga tcttccacta cttatccctc aaaatttata aattaaaaag ctttcggtca
    12061 cgctcatctg ttaaatactt atccatctga gcaattaatt tagctctggc ttgttcatca
    12121 tcttcagtga tctttagcat acaggatcca attaaaattt tgcgtctggt atcatccttt
    12181 cgagcttgtt ctttctcttt agcacgttct cttgccagtg ctgcctgtct ttgagccttt
    12241 aactgcttta gcttttcttc ctgtgctttg attttgctgt ctagtgtttc agtaacgctc
    12301 attaatccaa taccatcatc ctgggaaacc atccctaaca taagcgcact tgaatatgca
    12361 ttcaaggtgc tatatgtata gtaatgtttt cctcgaagag cgcacttatg caaacttcgt
    12421 ttgcaagtcc gattggggga accccaatac cccaacaacc gtatccacgg ttgtatttca
    12481 ctacgtgaaa atttaaaagc gaaaaacaag catggcgatt taccactttt cagtcaaaac
    12541 cgtagcacga tcagcagggc gttccgccac tgccgcgatt gcctatcggg caggtgaaaa
    12601 gatctattgt gaacgtgaag gccgagaaca tgactacagc cgaaaaaccg gggtggaata
    12661 caaagagatt tacttacccg aaggcgcacc agaacattta aaaaaccgag aaaaactctg
    12721 gaacgaagtc gaacaaagag aaaccagaaa aaactcaaca gtggcccgcg aatttgaaat
    12781 tgcctttcca agtgaattaa accaggaaca acgtctggcc atgctcgaag aattatgtgc
    12841 cagcatcgtg gaaagacacc aaatagccgt cgatgcctgc atccatgcac cacataccgg
    12901 atccggcagt gatgagcgca attatcacgc ccatatcctg atgagcacac gtaaactcac
    12961 gccagaaggt tttacagaaa aaacccggga actcgatcag aaacacagtg gagaaatcga
    13021 gcactggcgc gaacactttg cagatatctg caatatgcac ctcgacatgg ccggatccac
    13081 agcaagggtt gaccaccgca gctacaaaga ccaggagaat ggcttggaag cgaccctgca
    13141 cgaaggacca aaagtcaccg aactacgcag aagaggcatc gaaaccgaaa tcagccgaac
    13201 caacgatgaa atcaaagaaa gaaatcaggc tcagctgcaa tacggcaaaa atatggatct
    13261 actgattgct gaaaatgaga tcacactcaa aaaactaaaa acagaacagc agatgcatat
    13321 ccaaaatagc gctaaaacgc caccattaag cgcagaagag caatttcaat atatacagag
    13381 agaaaaccta agcaaactac tcaaaggcga aatcagcgca aaagaggcga atttagacct
    13441 agattttatg caacacaatc ttgagaaagt cgaaggtatc ctgagcaaac aacaaaagca
    13501 caaaaatgac ttcaatcagc agctcgccca agacatcgtg aaaagcagac tcaaacaaag
    13561 tcatgacaaa ttgcaaagcc tggtggatca acacaacgag ttaggtcaga acaagccctt
    13621 gctgtttgga aaaaaagctt gggaggcaca acgtgatgcg atttaccagg aacacaaaca
    13681 actgaaagat agacatgaat accagaaaaa aaatggtgtg aaggatttat tggaaaacga
    13741 aaaattcaaa gcacatgcct ggacacagta ccaaaaagag catccagcca aagccgagca
    13801 atatcagaac ctctaccaat cctaccgagc tattaaaaac ggtgtggata agatcaaagc
    13861 agaacaacaa atgaaactta gacaagaaca acagctcaaa gcacaacaac aagctcctaa
    13921 aatgaaatct cgtggcatga ggcgataaac agagcgagtg tctacgagcg aacagaagat
    13981 atctaaattt ttcgcccgcc ttttccctga ttacaagctc cctatccatc aatcacctgc
    14041 tttacagagc atcccgaatg ggtgcgaact ttccaagttt gcttcctaaa ttgagcgaag
    14101 cgagaaaaaa agctgatgag cgaagcgaat tccgagttgc ttttgatctt gcttttgctt
    14161 tttcttaaag tcacgaaaat atgaaccaaa aattgctccg acgaatcgag cgaaagcgag
    14221 attcaataga gtttgagcga acgaagtgaa gcgaaaacca agggcaattt ttcactcctt
    14281 tggcttttaa ttattttaaa gtttttaaat gcttttagat ggtctaaaaa cctttattta
    14341 taatattctt agagattata aaacgaggtt taccttgcat taaaacgagg tttaccttgc
    14401 attaaaacga ggtttacctt gcattaaaac gaggtttacc ttgcattaag cgagttgata
    14461 atataacctc gtcttattga attaaaaaca taatcttttc at
//
