LOCUS       NZ_CP032267             3470 bp    DNA     circular CON 05-JAN-2024
DEFINITION  Acinetobacter sp. WCHAc010034 plasmid p10_010034, complete sequence.
ACCESSION   NZ_CP032267
VERSION     NZ_CP032267.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN05356835
            Assembly: GCF_001696615.3
KEYWORDS    RefSeq.
SOURCE      Acinetobacter sp. WCHAc010034
  ORGANISM  Acinetobacter sp. WCHAc010034
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter.
REFERENCE   1  (bases 1 to 3470)
  AUTHORS   Hu,Y., Long,H., Feng,Y. and Zong,Z.
  TITLE     The complete genome of Acinetobacter sp. strain WCHAc010034
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3470)
  AUTHORS   Hu,Y., Long,H., Feng,Y. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2018) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
REFERENCE   3  (bases 1 to 3470)
  AUTHORS   Hu,Y., Feng,Y. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAR-2017) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.6
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology             :: Illumina HiSeq; MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/04/2024 21:32:37
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,697
            Genes (coding)                    :: 3,629
            CDSs (with protein)               :: 3,629
            Genes (RNA)                       :: 112
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 21 of 68
            Pseudo Genes (incomplete)         :: 53 of 68
            Pseudo Genes (internal stop)      :: 13 of 68
            Pseudo Genes (multiple problems)  :: 16 of 68
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP032267.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3470
                     /organism="Acinetobacter sp. WCHAc010034"
                     /mol_type="genomic DNA"
                     /strain="WCHAc010034"
                     /isolation_source="hospital sewage"
                     /db_xref="taxon:1879049"
                     /plasmid="p10_010034"
                     /geo_loc_name="China"
                     /collection_date="2015-10-31"
     gene            join(2721..3470,1..383)
                     /locus_tag="BEN74_RS00005"
                     /old_locus_tag="BEN74_00005"
     CDS             join(2721..2973,2973..3470,1..383)
                     /locus_tag="BEN74_RS00005"
                     /old_locus_tag="BEN74_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032072965.1"
                     /ribosomal_slippage=""
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISAba14 family transposase"
                     /protein_id="WP_223155595.1"
                     /translation="MARRPRRNHSNDFKAKVALAAIKAEKTLAELSAEFDVHQNQIIDW
                     KNQLISASSQAFDQSKAPTEPPIDLKKLHAKIGEQALEIGFFRRCVEETGPLQPQKLID
                     DSLQISVSKQAKLLKVSRGCYYYRPKPVSASDLKLMRCIDELHMQYPFAGSRMMRDLLN
                     RQGHHIGRRHTRTLMKKMGIQALYCKPNLSQANQAHRKYPYLLKGLAIQRSNQVWSTDI
                     TYIPMAKGFVYLCAVIDWHSRKVLAHRVSISMEVDFCISALNEAIEKYGRPEIFNTDQG
                     SQFTSDAFIDVLKSNGIQISMDGKGRWVDNVMVERLWRSVKYEEVYLKAYSSVTDAKKQ
                     LSAYFEFYNLKRPHSSLDKMTPNEFYYDQLPQQNKVA"
                     /name="IS3-like element ISAba14 family transposase"
     gene            468..875
                     /locus_tag="BEN74_RS00010"
                     /old_locus_tag="BEN74_00010"
     CDS             468..875
                     /locus_tag="BEN74_RS00010"
                     /old_locus_tag="BEN74_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004893891.1"
                     /GO_function="GO:0005515 - protein binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="WP_068913323.1"
                     /translation="MIIKAQVTWLSQFWYLLFGILFILSSFGSKSLVGVVVGGVLIAIA
                     AIHVLTTELALTNRRIIAKSGLIRRNTIELKTNRVESLGVNQGILGRIFNFGSIVVKGV
                     GGSHAPIPYIARPMEFRQQVNNYLDELDDKK"
                     /name="PH domain-containing protein"
     gene            complement(992..1966)
                     /gene="repM"
                     /locus_tag="BEN74_RS00015"
                     /old_locus_tag="BEN74_00015"
     CDS             complement(992..1966)
                     /gene="repM"
                     /locus_tag="BEN74_RS00015"
                     /old_locus_tag="BEN74_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846161.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepM"
                     /protein_id="WP_068913325.1"
                     /translation="MSDLIVKDNALMNASYNLDLVEQRLILLAILEARESGKGINANDP
                     LTVHAESYINQFGVARQTAYQALKDACKDLFARQFSYQEKRERGRANITSRWVSQIAYI
                     DETATVEVIFAPAVVPLITRLEEQFTQYDIEQISGLSSAYAVRLYELLICWRSTGKTPV
                     IELAEFRKRLGVLNDEYTRSDNFKKWIIENPIKQINEHTDITASYEQHKKGRTITGFSF
                     KFKQKKKTEPETPKNSDSSQRIEKPSQIPANIIKQPENANLSDLQHRASKITGLIMSNR
                     LSDRFKQGDESIMQMMARIQSEITTDSIAEQWQSKLEEFGVVF"
                     /name="repM"
ORIGIN
        1 cgattagtat ggaggtggat ttttgtattt cggctttaaa tgaagcgatt gaaaaatatg
       61 gtcgacctga aatatttaat acagaccaag gcagccagtt taccagtgat gcatttattg
      121 atgtattgaa atcaaatggc attcaaatca gtatggatgg taaaggtcga tgggtagata
      181 atgtgatggt tgaacgatta tggcggagcg ttaaatatga agaggtgtat ctcaaagctt
      241 atagcagtgt cacagatgcg aaaaagcaat taagtgcata ttttgagttt tataatttga
      301 aacgacctca ttcgagtcta gacaaaatga caccaaatga gttttactat gatcagctac
      361 cccaacaaaa caaggtggct taactagagc ggaatatcac ttataaatac gcttttagtt
      421 gttcaaacaa gtgggaccac ctctaaaact tagctagaga tgaacaaatt attataaaag
      481 cacaagttac ttggctatct cagttctggt atttattgtt tggtatctta tttattttat
      541 cttcttttgg ttctaaaagt ttagttggcg ttgtagtcgg tggtgtattg atagctattg
      601 cagcaattca tgtcttaaca acagaactag cattaacgaa tagacgaatt attgcaaaat
      661 caggattaat tcgaagaaat actattgaat taaaaactaa tagagttgag agtttagggg
      721 ttaatcaagg gatattagga agaattttca actttggttc tattgttgtc aaaggtgttg
      781 gtggatcaca tgcaccaatt ccatatatag ctcgacctat ggaatttaga caacaagtaa
      841 acaattacct tgatgaacta gatgataaaa aataaattaa cgatttaatc gcaaaaataa
      901 aaaagcgtca ctatcagtag tgacgctttt tgaaatgtaa taacagcaag cctttcaaac
      961 ataattttct acgtgattgc cacgtgacag actagaaaac cacaccaaat tcttctaatt
     1021 ttgattgcca ttgctctgct attgaatcgg ttgtaatctc actttgaata cgtgccatca
     1081 tctgcatgat tgattcatca ccttgtttaa accgatctga tagacggtta gacatgatta
     1141 accctgtaat cttgcttgct cgatgctgta aatcgctcaa atttgcgttt tcaggttgtt
     1201 ttatgatgtt ggcagggatt tgactaggct tttcgatacg ttggctagaa tcgctatttt
     1261 ttggcgtttc aggttctgtt tttttcttct gtttaaactt gaatgaaaac cctgtgattg
     1321 tccgcccttt tttgtgctgt tcgtagcttg ctgtgatgtc ggtatgctcg tttatttgtt
     1381 taatagggtt ttcaataatc cattttttga aattatccga tctagtatat tcatcattta
     1441 aaacacctaa acgctttcta aactctgcta gttcaataac aggcgttttc cctgtactac
     1501 gccaacaaat caataattca tatagacgga cggcataggc actcgataaa ccgctaattt
     1561 gttcaatatc gtattgggtg aattgttcct ctaatcgtgt aatcagtgga acaacggcag
     1621 gggcaaaaat aacctcaact gttgccgttt catcaatgta agcaatttgc gatacccaac
     1681 gacttgtaat attagctcgc ccacgctcac gtttttcttg gtaactaaat tggcgtgcaa
     1741 ataagtcctt acaagcatct tttaatgctt gatatgcagt ctgtctagcc acaccaaatt
     1801 gattgatata gctttcagca tgaacggtta gaggatcatt ggcattgatt cctttgcctg
     1861 attctctagc ctcaaggata gccaagagaa ttaaacgctg ctcaactaag tctaggttat
     1921 aactagcgtt cattaaggcg ttatctttta ctattaaatc actcatttta cttattaata
     1981 ttaaacctag taattccttt gtattatatt ttctagggtt atgcaaggta aacctaggag
     2041 tattggtagg taaacctagg agtattggta ggtaaaccta ggagtattgg taggtaaacc
     2101 taggagtatg tcctctgtat gccttgctgt gtaggcattt cagcatgtct aaaagcattt
     2161 aaaagcattt aaaaacttta aaagcccatt tacatcatga aaattgccct atgttttcgc
     2221 tttgctcaaa cttttttgac taagcttgct tagtcgaggg gcaatttctt caaattacgg
     2281 agaaaagttc aaaggtttaa aaagcataga gcaaggatac acagagggta ctttttcact
     2341 accgtgtaaa attcctactc tgtgccttgt tggggcaagc cccaaacccc aaaagcaaga
     2401 tcaaaaacaa aaatggcact cgcttcgctc gtgtcttctc aaaagcaaaa gcacacgata
     2461 gttaaaattt tcatgaaatc agatactaaa aaagcggttt ttcagagggg gaataattga
     2521 actttcatta tgtcgctcgt agacactcgc taaattattt tttgaacata taatcacttt
     2581 aaaattattt acatgtttaa agagggttta tgggaagtta tatcgaagaa aaagagatgg
     2641 atccgtaaat ttgaacaact cctataagtg atattctgct cctcaaatga tgttataaac
     2701 atcaatatat ggagtatttt atggcacgta gaccaagaag aaatcattca aatgatttta
     2761 aagctaaggt agcacttgct gcgattaaag cagaaaaaac acttgctgaa ttgagtgctg
     2821 agtttgatgt tcatcaaaac caaattattg actggaaaaa tcaattgatc tcagcttcct
     2881 cgcaagcttt cgatcaatca aaagctccaa cagaaccacc catcgatcta aaaaaactac
     2941 atgcaaaaat cggtgagcag gcattagaaa ttgatttttt agaaggtgtg ttgaagaaac
     3001 tgggccgctt caaccacaaa agttaatcga cgactcactt cagatttcag tatctaagca
     3061 agctaagctg ctgaaagtct cccgtggttg ttattactat cgcccaaaac ctgtgagtgc
     3121 atcagatctg aagctgatgc gatgtattga tgaattacat atgcaatatc cttttgcagg
     3181 cagtcgtatg atgcgtgatt tgttgaatcg tcaaggacat catataggac gacgtcatac
     3241 acgtacttta atgaagaaaa tgggtattca ggcgttatat tgcaaaccaa atttaagcca
     3301 ggctaatcaa gctcaccgta aatatccata tctgctcaaa ggattggcta ttcagcgcag
     3361 taatcaagtg tggtctacgg atataacgta tatccctatg gcaaaaggct ttgtttattt
     3421 atgtgctgtg attgattggc atagccgcaa ggtacttgcg catagagtat
//
