LOCUS       NZ_CP032116             4861 bp    DNA     circular CON 08-JAN-2024
DEFINITION  Acinetobacter lwoffii strain ED23-35 plasmid pALWED1.7, complete
            sequence.
ACCESSION   NZ_CP032116
VERSION     NZ_CP032116.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN05251102
KEYWORDS    RefSeq.
SOURCE      Acinetobacter lwoffii
  ORGANISM  Acinetobacter lwoffii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter.
REFERENCE   1  (bases 1 to 4861)
  AUTHORS   Mindlin,S., Petrenko,A., Kurakov,A., Beletsky,A., Mardanov,A. and
            Petrova,M.
  TITLE     Resistance of ancient and modern Acinetobacter lwoffii strains to
            heavy metals and arsenic revealed by genome analysis
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4861)
  AUTHORS   Beletsky,A., Kurakov,A., Petrova,M. and Mardanov,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2018) Lab of molecular cloning, Institute of
            Bioengineering, Research Center of Biotechnology of RAS, Prosp.
            60-let Oktyabrya, bld 7-1, Moscow 117312, Russia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Newbler v. 3.0
            Expected Final Version            :: yes
            Genome Coverage                   :: 30.2x
            Sequencing Technology             :: 454
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/08/2024 03:19:26
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 92
            CDSs (total)                      :: 92
            Genes (coding)                    :: 85
            CDSs (with protein)               :: 85
            Genes (RNA)                       :: 0
            tRNAs                             :: 0
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 3 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            Pseudo Genes (multiple problems)  :: 4 of 7
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP032116.1.
            Bacteria and source DNA available from Dr. Petrova M.A.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4861
                     /organism="Acinetobacter lwoffii"
                     /mol_type="genomic DNA"
                     /strain="ED23-35"
                     /isolation_source="permafrost, Kolyma lowland"
                     /db_xref="taxon:28090"
                     /plasmid="pALWED1.7"
                     /geo_loc_name="Russia"
     gene            1..609
                     /locus_tag="ABWED_RS00415"
                     /old_locus_tag="ABWED_E0006"
     CDS             1..609
                     /locus_tag="ABWED_RS00415"
                     /old_locus_tag="ABWED_E0006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004282301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="WP_004282301.1"
                     /translation="MELYTIILYSFFCFIGAILPGPTSLLALNLGLNNSIKAVFLASIG
                     AAIADFLIICAIGFGLKQIIDDFPIIFEVIKTLGFFYLIFIAYMIWKSENSISEHKDDT
                     HNIYRLPIKGFLTAISNPKVLVFFIAFLPQFIDPNLNISYQYIVLGIASSMIDIICMTI
                     YGLLGVKLLYFFKDKTNLLYLNRISAICMASIAMFLIIR"
                     /name="LysE family translocator"
     gene            complement(610..1029)
                     /locus_tag="ABWED_RS00420"
                     /old_locus_tag="ABWED_E0001"
     CDS             complement(610..1029)
                     /locus_tag="ABWED_RS00420"
                     /old_locus_tag="ABWED_E0001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005098409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="WP_004282303.1"
                     /translation="MNNEWLFEKITYISALKNPNDAQKLLLELAKIQYRTPDQEKKINA
                     LIKAEKAIDRANKQKVAVRKLLNAEKEAERKARTRHLIQLGALFEIANLDQRDPAELLG
                     VLLKTAEIDPNDMKWEIWKDLGQAVLNHNKKKNRV"
                     /name="conjugal transfer protein TraD"
     gene            1307..1462
                     /locus_tag="ABWED_RS00450"
                     /old_locus_tag="ABWED_E0002"
     CDS             1307..1462
                     /locus_tag="ABWED_RS00450"
                     /old_locus_tag="ABWED_E0002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005098410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_171770275.1"
                     /translation="MQLKQVLGKIKDITFGVFAAPLLIIIMLWASVLDQRAFKKERNRK
                     KEQDKP"
                     /name="hypothetical protein"
     gene            1462..2391
                     /locus_tag="ABWED_RS00425"
                     /old_locus_tag="ABWED_E0003"
     CDS             1462..2391
                     /locus_tag="ABWED_RS00425"
                     /old_locus_tag="ABWED_E0003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005249378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_087933487.1"
                     /translation="MAIARLSVKVGKAGKAAPHAEYIDRDEEKKLKQEQEQSDLEHSNY
                     GNMPKWAEHNPITFWQAADLYERKNGSTYREYEIALPREMNTEQRLELVEDFIQSEIGS
                     KYPYQFAIHNPKAMDGKDQPHVHLMFNERLQDGIERDPEQYFKRYNSKNPERGGAKKDN
                     TGKSYQERKTDIKDLRQRWEDLCNSHLEKHQIDSRIDMRSYKEQGIEKEPEKKLLPSQA
                     KDPEIREALQQSRTAYKELERLDLGDPKKDLKELKDSPISDKEIKQGIENFKADFDSFK
                     QLALQQYKEQQKLEREQQKTMKFRGMSR"
                     /name="MobA/MobL family protein"
     gene            2388..2888
                     /locus_tag="ABWED_RS00430"
                     /old_locus_tag="ABWED_E0004"
     CDS             2388..2888
                     /locus_tag="ABWED_RS00430"
                     /old_locus_tag="ABWED_E0004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018680098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_086045010.1"
                     /translation="MTPDEKKLYALMAVAEQQQNLVNQAVKELQITKEHIQSTIAGQTH
                     HTVSKAVNEGLQDGTAELIKTAKALGRLKNSIESASDQFSWKLITTFLGVFSVFILTMT
                     WLFASYIKPLENIPKIESLKQQGIQFDIQSCAVGEESKPCVRVMKEQCGYGKHGDLCVI
                     DPK"
                     /name="hypothetical protein"
     gene            2970..3785
                     /locus_tag="ABWED_RS00435"
                     /old_locus_tag="ABWED_E0005"
     CDS             2970..3785
                     /locus_tag="ABWED_RS00435"
                     /old_locus_tag="ABWED_E0005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005253922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004282298.1"
                     /translation="MLKQSSLERTEFKEEELAPIKKLDLNDLKVPMPKNLDQLLIWTSE
                     LAQYNYAQFLDQSEQLKLVDEDNLEEQAEISQKLRFYRGNALIENQIKDFYLKKLLDQG
                     EATVKGYIKSPTKKNTRYAVISYHDYDFVSFATPEILNKFPADLINFVQKETPKTLADI
                     YPEEAVFLSAIKKFIKPFKEQYKKLADKNREIKEHNTVAHKIYIKIKNKEATLQKPTES
                     KPKESSPKTTENSAVPVEMKQGTIQKNMAPQEKPLTVIKKRKFALNKDK"
                     /name="hypothetical protein"
     Typing          1463..2388
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.793"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atggaacttt atacaattat tttgtatagt tttttttgct ttataggggc cattttacct
       61 ggccctacat ctttattggc cttaaatcta ggtcttaata attctataaa agctgttttt
      121 ctagctagta ttggagcagc gatagctgat tttctgatta tttgtgctat tggttttgga
      181 ctaaaacaaa taattgatga ttttcctata atttttgagg taataaaaac tctaggattt
      241 ttctacttaa tttttatcgc gtatatgata tggaaatcag aaaacagtat ttctgaacat
      301 aaagatgata ctcataatat atatagatta cctataaaag gttttttaac agcaatttct
      361 aatcctaaag tacttgtatt ttttattgca tttttacccc aatttattga tcctaattta
      421 aatatatctt atcaatacat agttctaggt atcgcttctt cgatgataga tataatttgt
      481 atgacaatat atggattatt gggagttaaa ttattatatt tttttaagga taaaactaat
      541 ctactttatc ttaatcgtat aagcgctata tgtatggcta gtatagctat gttcctgata
      601 ataaggtaac tagactctat tttttttctt attatgattt aggacagctt gtcctaaatc
      661 cttccaaatt tcccatttca tatcatttgg gtcgatttca gcagttttaa gcagaactcc
      721 aagtaattct gcaggatcac gctgatctag gttagcaatc tcaaaaagtg cgcctaattg
      781 gataaggtgg cgtgtacgag cttttcgttc agcttccttt tctgcattca ataattttct
      841 aactgcaact ttttgcttat ttgctcgatc tattgctttt tcagctttga ttaaggcatt
      901 aatttttttc tcttggtcag gtgttctgta ctgaattttg gctaattcaa gtaaaagttt
      961 ttgagcatca ttagggttct ttaaagcaga aatataggtg attttttcaa atagccattc
     1021 attattcata tttaattcct tgattcagat accgcaaaat tacaggctca atactcaaag
     1081 aacgataaca aaatatttac gttcgagtaa cgtaattttg ttaaagtcgt tctagagatt
     1141 gtcgcctgca cttccatcaa aaatttgatg gcgcgcttag acgtttctca atttcattga
     1201 gaaacctaag cacgttaggg acttcatccc taaaaccctg aaacttcttt tttccttcaa
     1261 ttgaaaattg aatgaaaaaa gaagtaccaa agctaggagc aacagcatgc aactaaaaca
     1321 ggtattggga aaaatcaaag atattacttt tggtgtgttc gcagcaccgc tacttatcat
     1381 cattatgctg tgggcttcgg tacttgatca aagagcgttt aagaaagaac ggaaccgaaa
     1441 aaaagaacag gataaaccct aatggcgata gctcgtttaa gtgtgaaagt cggtaaggca
     1501 ggcaaggcag caccgcatgc cgaatatatt gatcgggatg aagaaaagaa gctgaaacag
     1561 gaacaggaac aatcagacct tgaacatagt aactatggga atatgccgaa atgggctgaa
     1621 cataatccga ttaccttttg gcaagcagcc gacctttacg agcgtaaaaa tggcagtacc
     1681 tatcgagaat atgaaattgc cctacctaga gagatgaata cagagcagcg tttagaactg
     1741 gttgaggact tcattcagtc tgagattggc tcaaaatatc cgtatcaatt tgcgattcat
     1801 aaccctaaag caatggacgg caaggatcag cctcacgttc atctcatgtt taatgaacgt
     1861 ctgcaagatg gaatcgaacg agatccggag cagtatttca aacgctataa cagcaaaaat
     1921 cctgaacgtg gcggagctaa aaaggacaat accggtaaga gttaccagga acgaaaaact
     1981 gatattaaag acctacgcca aagatgggaa gatttatgca acagccattt agaaaaacac
     2041 caaattgata gccgtattga tatgcgaagc tacaaagagc aaggcataga gaaagaacct
     2101 gaaaagaaac tattaccaag ccaagccaaa gaccctgaaa tcagggaagc cttacaacag
     2161 tcacgtacag cctataaaga gcttgagcga ctagacttag gtgacccaaa aaaagacctg
     2221 aaagaactca aagatagccc aatttcagac aaagaaatta aacagggtat tgagaacttt
     2281 aaggctgatt ttgatagctt caaacaactt gccttacagc agtacaaaga acagcagaaa
     2341 ttagaacgag agcagcagaa aacaatgaag tttagaggga tgagccgatg acacctgatg
     2401 aaaaaaagtt atatgcactg atggcagtcg cagaacaaca gcagaattta gttaatcagg
     2461 ctgtcaaaga gcttcaaatc acgaaagaac acattcaaag cactattgct gggcaaaccc
     2521 accatactgt atcaaaagcg gtaaatgagg gcttacagga cggtacagca gaacttatta
     2581 agaccgctaa agcacttgga cgcttgaaaa actcgattga atcagccagt gatcaatttt
     2641 catggaagtt aatcaccact ttcttaggtg ttttttcagt tttcatactg actatgacat
     2701 ggttatttgc atcttatatt aagcctttag agaatattcc aaagatcgag agtttaaagc
     2761 agcaaggtat tcagtttgat attcaaagtt gtgccgttgg agaagagtcc aagccttgcg
     2821 tccgagtgat gaaagagcag tgtggctatg ggaagcatgg ggatttatgc gttattgacc
     2881 ccaaatagct gaatctgaaa atgaaggctc taagcactca tttataattt ttattatgtt
     2941 taaaattagt ggaagttaag gaaattatta tgttaaagca atcaagttta gaaagaacag
     3001 agtttaagga agaagaactt gcgcccatta aaaagcttga tttgaatgat ttgaaagttc
     3061 ctatgcctaa aaaccttgat cagttactga tttggacatc cgaacttgct caatataatt
     3121 atgctcaatt ccttgaccaa tccgaacagc taaaacttgt tgatgaagac aacctggaag
     3181 aacaggcgga aatcagtcag aagcttaggt tctatagagg taatgcgctt atagaaaacc
     3241 aaatcaaaga tttctatttg aaaaagttac ttgatcaagg ggaggcaaca gtcaaaggtt
     3301 atattaaaag tccgactaag aaaaataccc gatatgctgt aatttcttat catgactatg
     3361 attttgtaag ctttgctact ccagagattc ttaataaatt tccagccgat ctcatcaatt
     3421 tcgtgcaaaa ggaaacacct aagacattag cggacatata tcctgaagaa gccgtgttct
     3481 tatcagctat caaaaaattt ataaaacctt ttaaagaaca gtataaaaaa ttggctgata
     3541 agaacaggga aattaaggag cacaatacag ttgctcataa aatttatata aaaattaaaa
     3601 ataaggaagc aactctgcaa aagccaacag agtcaaagcc taaagaatct agtccaaaga
     3661 ctactgaaaa tagtgctgtc ccagtagaga tgaagcaggg aaccattcag aaaaatatgg
     3721 ctccacaaga aaagccactt actgtgatta agaagcgaaa atttgcttta aacaaggata
     3781 agtaacttgt aatgtaaaat tctaaacaaa aacccctcta agtcttgcaa cttagagggg
     3841 ttttcaagta aattcagcgg tacgcagtta tttgatgcta caacatcaag taacgaagat
     3901 cataaggttt gacgaccgtt atgatttcac caaaaggctt gtacctgaac tctattttca
     3961 caataaacag tgtttatatc aagcctcaaa atgataagtc ttcgttaatt cgtgttttaa
     4021 accatcaaat aagcatccta gatcgagctt tgcccgcttc tgcgtgtccg ttttacggtc
     4081 ggcatgcatt acgtgtggta ggaataagtg tcactggcgt attgtagcta tagtcgaaag
     4141 gctaaaccgc ttacagggga acttcacata ggcggagcgt tgggagtagt tacataggac
     4201 ttgttaagtt gcatgttggg gaaaccctcg cagagataca ggcaatgtaa cgacagggag
     4261 cgcgtggagc gaggtgcaaa actttgagtc cttgtctgca tagtcagcaa aactatgtgc
     4321 ggatacgacc gatatacgtg gctccgaagc caaatatata caggtaaatc ggcaatactt
     4381 aggggatgtg tcattttttt aaatgactgc ccttagggtg agtattgccg aaactctctc
     4441 aaccgtttcc aaagccaaag cacgaagtgc gacgctttgg aaacggttga gaggttgagt
     4501 tcatttgttt ttcttttata tatgagcttt agaaaaaact aacttgcctt aggctatttt
     4561 tttattttac ttttcttgtg aaaagtttta ctttattttc ctccaattac tacaaatgaa
     4621 gaagattatt atggagactt tgatatagag ttttctaatt tctggacaaa tagcaattac
     4681 tactgcatgt ttacagggtt taaatcctat ccaaactcag tatatccaaa aatactttat
     4741 gataataaat atgataaagc tcgagaatat atgcaagagt taaagaaacg tgaggataat
     4801 ctcttggaaa gtttaccaag tacttatgaa tacctaaaac atctacacag tagaaaataa
     4861 t
//
