LOCUS       NZ_CP032115             5596 bp    DNA     circular CON 08-JAN-2024
DEFINITION  Acinetobacter lwoffii strain ED23-35 plasmid pALWED1.6, complete
            sequence.
ACCESSION   NZ_CP032115
VERSION     NZ_CP032115.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN05251102
KEYWORDS    RefSeq.
SOURCE      Acinetobacter lwoffii
  ORGANISM  Acinetobacter lwoffii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter.
REFERENCE   1  (bases 1 to 5596)
  AUTHORS   Mindlin,S., Petrenko,A., Kurakov,A., Beletsky,A., Mardanov,A. and
            Petrova,M.
  TITLE     Resistance of ancient and modern Acinetobacter lwoffii strains to
            heavy metals and arsenic revealed by genome analysis
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5596)
  AUTHORS   Beletsky,A., Kurakov,A., Petrova,M. and Mardanov,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2018) Lab of molecular cloning, Institute of
            Bioengineering, Research Center of Biotechnology of RAS, Prosp.
            60-let Oktyabrya, bld 7-1, Moscow 117312, Russia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Newbler v. 3.0
            Expected Final Version            :: yes
            Genome Coverage                   :: 30.2x
            Sequencing Technology             :: 454
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/08/2024 03:19:26
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 92
            CDSs (total)                      :: 92
            Genes (coding)                    :: 85
            CDSs (with protein)               :: 85
            Genes (RNA)                       :: 0
            tRNAs                             :: 0
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 3 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            Pseudo Genes (multiple problems)  :: 4 of 7
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP032115.1.
            Bacteria and source DNA available from Dr. Petrova M.A.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5596
                     /organism="Acinetobacter lwoffii"
                     /mol_type="genomic DNA"
                     /strain="ED23-35"
                     /isolation_source="permafrost, Kolyma lowland"
                     /db_xref="taxon:28090"
                     /plasmid="pALWED1.6"
                     /geo_loc_name="Russia"
     gene            664..1608
                     /gene="repM"
                     /locus_tag="ABWED_RS00375"
                     /old_locus_tag="ABWED_D0001"
     CDS             664..1608
                     /gene="repM"
                     /locus_tag="ABWED_RS00375"
                     /old_locus_tag="ABWED_D0001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004282268.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepM"
                     /protein_id="WP_159373074.1"
                     /translation="MKNELVVKDNALINASYNLEVTEQRLMLLAILNARETGNGITADS
                     KLEIHAKDYANRFDISNDGAYKALKNAVLNLFDRQFSFKEQDKKGNVGTVKSRWVSRIK
                     YIDDSATLEITFAPDVVPLITRLEQHFTSYQLKQVSQLTSKYAIRLYELLIAWREVGKT
                     PKIELDDFRSKIGVEDTEYTAMNDFKKRVLEPAIRQINKYTDITVTYEQYKKGRLISAF
                     SFSFKQKHQPRIEKNVNLKRDPNTPDFFIPLTDAQRHLFADKLSEMPEMSKYSQGTESY
                     QQFAIRIAKMLLEPEKFRELYPVLKKAGFQEYL"
                     /name="repM"
     gene            1696..2268
                     /locus_tag="ABWED_RS00380"
                     /old_locus_tag="ABWED_D0002"
     CDS             1696..2268
                     /locus_tag="ABWED_RS00380"
                     /old_locus_tag="ABWED_D0002"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_159373075.1"
                     /translation="MKKTILRIVLALTCLSLVFIGFIFIAYFLDRQKQKEEMVLLDSLQ
                     KELRPDIDSYPKLKKYYLDLFNKGLISQDIDASYKPMYQRVEEAELITLNCSIKIYDLT
                     EDELALGLKKFDNEINTEGYDSLHSIMEDINKIPPIARVFTEYKADNKITDRELCTLAN
                     MYERGLNYQSNLIENEFNHQTKSEVLQ"
                     /name="hypothetical protein"
     gene            complement(2279..2548)
                     /locus_tag="ABWED_RS00385"
                     /old_locus_tag="ABWED_D0003"
     CDS             complement(2279..2548)
                     /locus_tag="ABWED_RS00385"
                     /old_locus_tag="ABWED_D0003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005207691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="WP_159373076.1"
                     /translation="MTKAAETLEKKIEAQLEKLKQLKARKQAIEAREKSKQKEQERKDD
                     TRRKILLGSYLIKKMNDNEANKEKILAELNEYLTEDRDRKLFNL"
                     /name="mobilization protein"
     gene            2730..2957
                     /locus_tag="ABWED_RS00390"
     CDS             2730..2957
                     /locus_tag="ABWED_RS00390"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_159373077.1"
                     /translation="MDKMMQNLENNLRKYKASWIALFCVIFLFILTIQLNNIANAARYA
                     GTDTSNLENVLDNINTNLDEIRKKYTDIYM"
                     /name="hypothetical protein"
     gene            2957..3910
                     /gene="mobQ"
                     /locus_tag="ABWED_RS00395"
                     /old_locus_tag="ABWED_D0004"
     CDS             2957..3910
                     /gene="mobQ"
                     /locus_tag="ABWED_RS00395"
                     /old_locus_tag="ABWED_D0004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017284732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_159373078.1"
                     /translation="MAIYHCTTKTVNRSSGRTAVASAAYRAGEKLEDKRTGLTHDFTKK
                     EGVAYTEIISNLDTQIERAELWNMAEKTENRKDARTAREWVIALPDELDEEQRKELAKD
                     FAKSLVDRYGVVADLAIHAPSKGGDDKNHHAHILLTTRKAELDHENKLVLTQKAEIELS
                     NTKRKSLGMGTSQEEIKQIRATWANLANYALDKAGYKEKIDHRSYAEQGNGLQATIHEG
                     SKVTQLRRKGIDTEVSRFNDNIKQQNSQQLQYKESQKEKILEQGFNRVEKGFEQWKKDQ
                     EAKRLQLEHQQQLKLQQERAMKLKQRKSMNRNGPSL"
                     /name="mobQ"
     gene            3907..4377
                     /locus_tag="ABWED_RS00400"
                     /old_locus_tag="ABWED_D0005"
     CDS             3907..4377
                     /locus_tag="ABWED_RS00400"
                     /old_locus_tag="ABWED_D0005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004282271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_159373079.1"
                     /translation="MSNQNDLDDQLYILLASMKEYREAIADDNKRLEMFYTQVASGVLD
                     KAEKSLQETNKQAIGALKSRIQELDKATSRLNYQFIAVFASAFVALVMVLFLALFLFVP
                     SMDEIQQRRSEVNNLKNYSLDLSKCDGKTCVRIIKKQCGYGKNGDYCVIDPK"
                     /name="hypothetical protein"
     gene            4527..4997
                     /locus_tag="ABWED_RS00405"
                     /old_locus_tag="ABWED_D0006"
     CDS             4527..4997
                     /locus_tag="ABWED_RS00405"
                     /old_locus_tag="ABWED_D0006"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_159373080.1"
                     /translation="MKNIILFICVLFPLICHAKTNDWQLIHAHYYPQDKEEKHIQKIYL
                     DKASIKTSDGDIREFTTINTFPNGYLNPSNKVEKKKDEYGTFIIKCSNATGWTDSWNID
                     TKSEKFGEVNKVWSPIFTKISVITNVVNIPALAFYDVCFDRISKDFNNIIFR"
                     /name="hypothetical protein"
     gene            complement(5147..5470)
                     /locus_tag="ABWED_RS00410"
                     /old_locus_tag="ABWED_D0007"
     CDS             complement(5147..5470)
                     /locus_tag="ABWED_RS00410"
                     /old_locus_tag="ABWED_D0007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019767300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_228708723.1"
                     /translation="MEGINMIPVLPAVDTHKAHILYRLLNRVPVKNKDMWSSIDSSYSA
                     CRISELRSDGWNIEDHYLSEDSKLKKRVRVKVYFMQPLLISELLKEKAIQDFLRRFEYR
                     QAS"
                     /name="hypothetical protein"
     Typing          2958..3907
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.114"
                     /coverage="100.0"
                     /evalue="6.78e-172"
ORIGIN
        1 ggtttgattt gttagctatt tgatattaaa gatttcaaaa aaggtacttg ttagcgcaag
       61 tccctttttt tttgctttat tttcaaacag ttcaaactct aacataggta tgcacaaaaa
      121 tacacccctt atgcgcacag tttcgcgtat ttcgaatgtt tacatgtaaa catatggttg
      181 tgtacccatt ttcttttaat cggccgtaag cggccttaca ggaagaatta gaattaatta
      241 taaaaaatta ttttcaattt tattaaataa aaaatccttt tttatctttt taataagctt
      301 ttaaaagctt ttagctcccc ttcaaaccct ttatttacaa ggctttcaaa ctttaaaaaa
      361 ctacaaatag aggacattaa gctacaccga tcttagggct gttttcatgt ctgtttaggc
      421 ttttatatat attttaaagt ttttaaatgc ttttaggctc gctaaaagcc gttctataaa
      481 aggcatttag cgctcataaa gggacattta gtcggttata aagggacatt tagtcggtta
      541 taaagggaca tttagtcggt tataaaggga catttgctct ttataaaggg acactatagg
      601 cattgaccct ttagtgactt caatttacac tgtctgcatt ataagtaaca atataatctg
      661 attatgaaaa atgagcttgt tgtgaaggat aatgcgttaa ttaatgctag ttataactta
      721 gaagtaactg aacaacgctt aatgctttta gcaattctta atgcaagaga aacaggtaac
      781 ggtattacag ccgatagtaa attagaaata catgcaaagg actatgctaa ccgctttgat
      841 ataagtaatg atggagctta taaagcatta aaaaatgctg tcttaaactt gtttgatcga
      901 caattttctt ttaaagaaca agataaaaaa ggaaatgtag gtacagtaaa atcacgatgg
      961 gtaagccgta ttaaatatat tgatgattca gccactctag agattacgtt tgcacctgat
     1021 gttgtcccac taattacaag acttgagcaa catttcacga gctatcaact taaacaagtc
     1081 agccaactta ccagtaaata cgcaatccgc ttatatgagc ttttaattgc atggcgtgaa
     1141 gttggaaaaa caccaaaaat tgaattggat gactttagaa gcaaaattgg tgttgaagat
     1201 actgaataca ctgccatgaa tgattttaaa aaacgtgtat tagagccagc tattaggcaa
     1261 attaataaat atacggatat tacggtaacg tatgagcagt ataaaaaagg acggttaatt
     1321 tcagcatttt cattcagctt taagcagaag caccagccaa gaattgaaaa gaatgtgaac
     1381 cttaaaagag atccgaatac acctgatttc tttatcccac tgacagatgc tcaacgtcat
     1441 ttatttgccg ataaactgtc cgagatgcca gaaatgagta aatattcgca aggtactgaa
     1501 agctaccaac aatttgctat acgtattgct aaaatgctgt tagagcctga aaaatttaga
     1561 gaactatatc cagttttaaa aaaagcagga ttccaagaat atttataaaa aataatttac
     1621 aagctcaact aaagggctaa ttgtttttta ttaaatgttc tacaatttta gtgatattta
     1681 tcattactgc ataacatgaa aaaaacaatt cttcggatag ttctagccct tacttgtctg
     1741 tcattagttt ttataggttt tatatttata gcttattttc ttgatagaca gaaacaaaaa
     1801 gaagaaatgg tgcttttaga ctcccttcaa aaagaactta gaccagatat tgactcatat
     1861 ccaaaactta aaaagtacta tcttgattta tttaataaag gtctcatttc tcaagatata
     1921 gatgctagtt ataagcctat gtaccagaga gttgaagagg ctgagcttat aacacttaat
     1981 tgttctataa aaatttatga tttaactgaa gatgagttag ctttaggtct gaaaaaattt
     2041 gataatgaaa ttaacactga gggctatgat tcacttcact caattatgga agatattaat
     2101 aagattccac ctattgctag agtctttact gaatataagg ctgacaataa aattacagat
     2161 agagaactat gcactcttgc aaatatgtat gagaggggat tgaattatca aagtaattta
     2221 attgaaaatg aatttaacca tcagactaaa tcggaagtac ttcagtaagt ataaaatttc
     2281 ataaattaaa tagttttcta tctctgtctt ctgttaaata ctcgttgagt tcagcgagta
     2341 ttttttcctt attagcttca ttgtcattca tctttttaat taaataagaa ccgagcaaaa
     2401 ttttgcgtcg agtatcgtcc tttctttcct gctctttttg cttcgacttt tctctagctt
     2461 cgatagcttg tttgcgagcc tttaactgtt ttagcttctc taattgggct tcaattttct
     2521 tttcaagagt ttcagcagct tttgtcatga ttcagatttc gggatataga aaaccactca
     2581 agcataaggc cctatgaatt gaaattcaag cgggctatat gctatcgtgt ttttcatgaa
     2641 atgcgcactt acgacttgga tttcatccaa gtcaaagttt gcgtaagggg aatcccctta
     2701 acccctaaaa actgctacgc aatttttaaa tggataaaat gatgcaaaat ttagaaaata
     2761 atttaagaaa atataaagca agttggattg cgctgttttg tgtaattttt ctttttattt
     2821 tgacaataca gttaaataat attgctaatg cagcgagata tgcaggcact gatacatcaa
     2881 acctagaaaa tgtattagat aatatcaata caaatttaga tgaaatccga aaaaaatata
     2941 cagatatcta tatgtaatgg caatttacca ctgtacgact aagaccgtga accgaagttc
     3001 gggacgaact gcggttgcat ctgctgcata tcgtgcagga gaaaagttag aagataaacg
     3061 gacaggatta acgcacgact ttacgaaaaa agaaggtgtg gcttataccg aaattatttc
     3121 gaatttagat actcaaattg agcgtgccga actctggaat atggcggaaa aaaccgaaaa
     3181 ccggaaagat gccagaaccg ctagagaatg ggttattgcc ttgcctgatg aattagatga
     3241 agaacaacgc aaggaacttg ctaaagactt tgccaagtcc ttagtagatc gctatggggt
     3301 agttgcagat ttagcgattc atgcacccag caaaggtgga gatgataaaa atcatcatgc
     3361 acatatcctg ctgacaactc gcaaagcaga attagaccat gaaaataaac tggtccttac
     3421 tcaaaaagcg gaaatcgagc taagtaacac caaaagaaaa agcctaggca tgggaacaag
     3481 ccaggaagaa atcaagcaga ttcgagccac ttgggcaaac cttgcaaatt acgccctgga
     3541 caaagcaggg tacaaagaaa aaatagacca tcgtagctat gccgaacaag gtaacggcct
     3601 acaagccacc atacatgaag gtagcaaggt tactcaatta cgcaggaagg gcatagatac
     3661 tgaagttagc cgtttcaatg acaacatcaa acagcaaaac agtcagcagc tccaatacaa
     3721 agaatcccaa aaggaaaaaa tcctagaaca aggcttcaac cgtgtcgaaa agggtttcga
     3781 gcaatggaag aaagatcagg aagctaaacg cttacaactg gaacatcagc agcagttaaa
     3841 gctacagcaa gaaagagcca tgaagctgaa acaacgcaaa agcatgaata gaaatgggcc
     3901 ttccctatga gcaatcaaaa cgatttagac gaccagttat atattttatt agcttccatg
     3961 aaggaatata gagaagccat agcagacgat aataagcgat tagagatgtt ttacacccaa
     4021 gtagctagtg gagttctgga taaagccgaa aagtcgctac aagagacaaa taaacaggct
     4081 ataggggcat taaagagccg tattcaggaa ttagataaag caacaagccg tcttaactat
     4141 caatttattg ccgtgtttgc cagtgccttt gtagcgttgg taatggtact gttcttggcg
     4201 ttatttttat ttgtaccaag tatggatgaa atccagcagc gcagatcaga agtgaataac
     4261 ctgaaaaatt acagcttaga cctatcaaaa tgcgatggga aaacatgcgt tagaattata
     4321 aaaaaacagt gtggctatgg gaaaaatggc gattattgtg taatagatcc caagtaatta
     4381 tcgttgttta ctttgctctg ttctatccag tccttatcaa atacctaatg tcgcaaagtg
     4441 gctcgacttt gaaacatggc taaagccaca tttcaaagta tcgagctaag acaaaaaact
     4501 tctatttatg taatttagac tatctcatga aaaatattat cctatttatt tgcgtccttt
     4561 tccctttaat ttgtcatgcg aaaacaaatg attggcagtt gattcatgcg cactactacc
     4621 ctcaagataa ggaagaaaag cacattcaga aaatctattt ggataaagct tcaatcaaaa
     4681 catctgatgg tgatattcgt gagtttacaa cgatcaatac attcccgaat ggttatttga
     4741 atccaagtaa caaggtcgaa aaaaagaaag atgaatatgg gacattcatt atcaagtgct
     4801 ctaatgctac gggatggact gatagctgga atattgatac taaaagtgaa aaatttggtg
     4861 aagttaataa agtatggtcg cctattttta cgaaaatatc agttatcacg aatgtggtga
     4921 acatccctgc actagctttc tatgatgttt gttttgatag aatttctaaa gatttcaaca
     4981 atattatatt tcgataatta ttagatattt gaagctagtc cagtaagaac agagcgagtg
     5041 tctacgagcg atcacctggc atcaaaattt atgcattttt ccctttgatt acaaaatctg
     5101 cttttttaaa gcaaaaaaaa ggaagggggt aacccttcct tttcatttaa ctggcttgtc
     5161 gatattcaaa ccttcttaaa aaatcttgaa ttgccttttc tttcagtaac tcactaatta
     5221 ataaaggttg cataaagtac acttttactc gcacacgttt cttcagtttt gaatcttcag
     5281 ataaatagtg atcttcaata ttccatccat cactccgtaa ttcgctaata cgacacgcag
     5341 agtaactaga atcaatggac gaccacatat ctttgttctt aacaggcacc ctatttaata
     5401 agcggtacaa gatatgtgct ttatgtgtat ctactgccgg taaaactgga atcatattga
     5461 ttccctccat cataaaaaat ggaggttaat cagccgtatc tgttcgctaa cttgaacaaa
     5521 ttgttcatca atgatagaat cgttcatgca atttaaacgt aaatcattaa ttcttatggc
     5581 tttttaaacc tccttt
//
