LOCUS       NZ_CP032113            14118 bp    DNA     circular CON 08-JAN-2024
DEFINITION  Acinetobacter lwoffii strain ED23-35 plasmid pALWED1.4, complete
            sequence.
ACCESSION   NZ_CP032113
VERSION     NZ_CP032113.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN05251102
KEYWORDS    RefSeq.
SOURCE      Acinetobacter lwoffii
  ORGANISM  Acinetobacter lwoffii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter.
REFERENCE   1  (bases 1 to 14118)
  AUTHORS   Mindlin,S., Petrenko,A., Kurakov,A., Beletsky,A., Mardanov,A. and
            Petrova,M.
  TITLE     Resistance of ancient and modern Acinetobacter lwoffii strains to
            heavy metals and arsenic revealed by genome analysis
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14118)
  AUTHORS   Beletsky,A., Kurakov,A., Petrova,M. and Mardanov,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-SEP-2018) Lab of molecular cloning, Institute of
            Bioengineering, Research Center of Biotechnology of RAS, Prosp.
            60-let Oktyabrya, bld 7-1, Moscow 117312, Russia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Newbler v. 3.0
            Expected Final Version            :: yes
            Genome Coverage                   :: 30.2x
            Sequencing Technology             :: 454
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/08/2024 03:19:26
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 92
            CDSs (total)                      :: 92
            Genes (coding)                    :: 85
            CDSs (with protein)               :: 85
            Genes (RNA)                       :: 0
            tRNAs                             :: 0
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 3 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 5 of 7
            Pseudo Genes (multiple problems)  :: 4 of 7
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP032113.1.
            Bacteria and source DNA available from Dr. Petrova M.A.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..14118
                     /organism="Acinetobacter lwoffii"
                     /mol_type="genomic DNA"
                     /strain="ED23-35"
                     /isolation_source="permafrost, Kolyma lowland"
                     /db_xref="taxon:28090"
                     /plasmid="pALWED1.4"
                     /geo_loc_name="Russia"
     gene            232..639
                     /locus_tag="ABWED_RS00245"
     CDS             232..639
                     /locus_tag="ABWED_RS00245"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_121980474.1"
                     /translation="MLLSYQVKSDHRKPQWKASEKSLWKVEECIELDIFSYGINSKWTT
                     NSGAKAIVWSYHRDSDSEKLVKIGEDHRRSSSIGIVDLGLAKFTCDHNNCWHGYPIDPA
                     TDSVPSSILKIWQNTLGKKLATKINQGKLKL"
                     /name="hypothetical protein"
     gene            636..1757
                     /locus_tag="ABWED_RS00250"
                     /old_locus_tag="ABWED_B0002"
     CDS             636..1757
                     /locus_tag="ABWED_RS00250"
                     /old_locus_tag="ABWED_B0002"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_125825060.1"
                     /translation="MSFTKVSLEGDFLEAFLYSGILFTVDTDGILSTCSFKNLINSYLD
                     KTRNKDSKIIKKYILNDNKINQSYDGENISLYITKKFIDENCVFNQVPLDVWPTDLDIK
                     SNTVYISSERGLDILPFHWDINSVSFSDEKEKLKNIWDDTKVFNLATGSWGRTVLAAGN
                     EGAIEIFNVYDSFYKNVIDSLCKPIKPKIISKGTWLECDWGYSKVLVLKNFDEQKAFLF
                     ESTAKQDVLENRKKENSENFEESVDELIRDLIKKPKNLEIKKENIVQYSIINGNIVGID
                     DKGNKYKFNLSKNKWQLTSEIKYESFLKDYSEIKFIYPGTIIESNEKIYIDKRNHLYQL
                     DDNFIKWRTFPRSKNYVNHTLIIKDNELDIYAL"
                     /name="hypothetical protein"
     gene            1972..2406
                     /locus_tag="ABWED_RS00255"
                     /old_locus_tag="ABWED_B0005"
     CDS             1972..2406
                     /locus_tag="ABWED_RS00255"
                     /old_locus_tag="ABWED_B0005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004282228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_121980251.1"
                     /translation="MKKIILTPIIALLLGLTGCSTQQVKPIPYNQKALNLKVGMTKDEV
                     INLLGTPKKVSAIKTNEGLEEVYSYWGISRLVYSYTPIDNEALSQDRLAVTFLDGKVTQ
                     WGDKFDPTVISNQTLKTIQQTSQQYQQMYEKQIQNTRENK"
                     /name="hypothetical protein"
     gene            complement(2676..3212)
                     /locus_tag="ABWED_RS00260"
                     /old_locus_tag="ABWED_B0007"
     CDS             complement(2676..3212)
                     /locus_tag="ABWED_RS00260"
                     /old_locus_tag="ABWED_B0007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004786457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication DNA-binding protein"
                     /protein_id="WP_121980252.1"
                     /translation="MKKLSVSELAKLYGYSRQAVYAHIKKGNLSKGSDGLIDFSEALRV
                     FGEPQKSNSSVNQSQSISRHNLTEVDSLKRQVDMLERQLNQAIQRENQSLERESFYQEQ
                     IEAMQRLLEAPKANMTTFTDHKSEQDIATDSRPQTESNYDGLTTPENKRIPVPEHIEPT
                     PKKRGFLSRFFLPYG"
                     /name="plasmid replication DNA-binding protein"
     gene            complement(3280..4203)
                     /gene="repM"
                     /locus_tag="ABWED_RS00265"
                     /old_locus_tag="ABWED_B0008"
     CDS             complement(3280..4203)
                     /gene="repM"
                     /locus_tag="ABWED_RS00265"
                     /old_locus_tag="ABWED_B0008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004282204.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepM"
                     /protein_id="WP_004282204.1"
                     /translation="MKTELIVKDNALINASYNLDLVEQRLILLAIVEARESGKGINAND
                     PLTVHAESYINQFGVHRNTAYQALKDACDDLFARQFSYQSLSEKGNVINHKSRWVSEVA
                     YIDNEAVVRLIFAPAIVPLITRLEEQFTKYEIQQISNLTSAYAVRLYEILIAWRSTGKT
                     PLITIYDFRQKIGVLETEYKRMYDFKKYVLDIALKQVNEHTDINVKVEQHKTGRSITGF
                     SFSFKQKKSATNTANDISQDKELKINLTDAQRYLFASKLSELPEMAKLSQGNESYEQFA
                     ARIVTMLQEPNKLKEFMPLLRKVGFQ"
                     /name="repM"
     gene            complement(4741..6177)
                     /gene="mobQ"
                     /locus_tag="ABWED_RS00270"
                     /old_locus_tag="ABWED_B0009"
     CDS             complement(4741..6177)
                     /gene="mobQ"
                     /locus_tag="ABWED_RS00270"
                     /old_locus_tag="ABWED_B0009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005248576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_121980253.1"
                     /translation="MAIYHFSVKTVARSAGRSATAAIAYRAGEKIYCEREGREHDYSRK
                     TGVEYKEIYLPEGAPKHLKNREKLWNEVEQRETRKNSTVAREFEIAFPSELNQEQRLAM
                     LEELCTSIVDRHQVAVDACIHAPHTGSGSDERNYHAHILMSTRKLTPEGFTEKTRELDQ
                     KHSGEIEHWREHFADICNMHLDLAGSTARVDHRSYKDQENGLEATLHEGPKVTELRRRG
                     IETEISRNNDEIKQRNQAQLQYGKNMDLLIAENEIKLSTLKTDQQIQIKNSAKTPPIDE
                     KALFEKKQRETLGKVLKREISAKDANLDLDFMQQHLEKAEKTLSKQHKHQNEFNQQLAQ
                     EIVKSGLKQSHDKLQSLVDQHNELTQNKPLLFGKKAWEAQRDEIYQEHKKLKGQHEHQK
                     KHGVKELLQNDAFKEHAWKKYQKQHPEKAKQYQTLYQSYKIVKKCVDEIKAEQQMKLRQ
                     EQQLKAQQHAPKMKSRGMSR"
                     /name="mobQ"
     gene            6388..6654
                     /locus_tag="ABWED_RS00275"
                     /old_locus_tag="ABWED_B0010"
     CDS             6388..6654
                     /locus_tag="ABWED_RS00275"
                     /old_locus_tag="ABWED_B0010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005268842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="WP_121980254.1"
                     /translation="MSITETLDSKIKAQEEKLKQLKAQRQAALARERAKEKQQTRKDDT
                     RRKILIGSCMLKITEEDEQARAKLIAQMDRYLTDERDRKLFNL"
                     /name="mobilization protein"
     gene            6669..6977
                     /locus_tag="ABWED_RS00280"
                     /old_locus_tag="ABWED_B0011"
     CDS             6669..6977
                     /locus_tag="ABWED_RS00280"
                     /old_locus_tag="ABWED_B0011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_077068019.1"
                     /translation="MNNLSITSPKAPLIKSWFGFVLGAASVAISLFGLSKSFDLAGILS
                     TLGFLCFWYPWSQMTGWKDPIKHFFKVDDQSMSKPCIYLTLIATILLITSTVITFIN"
                     /name="hypothetical protein"
     gene            7322..9199
                     /locus_tag="ABWED_RS00285"
                     /old_locus_tag="ABWED_B0013"
     CDS             7322..9199
                     /locus_tag="ABWED_RS00285"
                     /old_locus_tag="ABWED_B0013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003653920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="WP_004282101.1"
                     /translation="MQNNVSKPAVPALTLAALGVVFGDIGTSPLYALKESFHATRGLPI
                     NELNILGILSLIFWTITLIVSLKYVLVIMRADNNGEGGIMALLALNLRQKGLSRGKRLA
                     IIILGFIGASLFFGDGIITPAISVLSAVEGLSVAAPSFDRFIIPISIGILIALFAVQKH
                     GTAMMGKFFGPITLLWFLSIGAIGIMSIIQSPFVLALFSPHWAFLFVINNPLISFFVMG
                     AVVLTVTGGEALYADMGHFGILPIKLGWFLIVLPCLMLNYAGQGALLLRDPSAITNPFY
                     LLLPSALLYPMIILATAAAVIASQALISGVFSMAKQAIQLGYLPRLTVLHTSASEIGQI
                     YIPLLNWLLFISITILILIFESSSKLAGAYGLAVTVTMFCDTLLVAFLAYSYWKWKTWK
                     VLLFIIPFAFIDLVLLSSNLLKVLIGGWVPVVIAVIVFTLMMTWKKGREILQDKLQKDT
                     LPLNVFLEHLEQTGQKVSGNAVFLTGTPQVVPHALLHNLKHNKVLHERNFLVTIKTSEI
                     PYVDEAKRIVTEVLENGFFRITIHYGFKEEPNVPHSLKQAFSVLDLEYDLMNISFFVSR
                     ERLIPSLSSKMSTWREKLFVAMQKNTSPVSDFYKIPSNRVVELGSQIEI"
                     /name="potassium transporter Kup"
     gene            complement(9266..9463)
                     /locus_tag="ABWED_RS00290"
                     /old_locus_tag="ABWED_B0014"
     CDS             complement(9266..9463)
                     /locus_tag="ABWED_RS00290"
                     /old_locus_tag="ABWED_B0014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005265040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004282105.1"
                     /translation="MFNFKIFNKVSTEVLTIKNDLQLNSEIQLITKYKTSTSEDYKKAI
                     ILIFKERGYSALEIGQLLEN"
                     /name="hypothetical protein"
     gene            complement(9679..9981)
                     /locus_tag="ABWED_RS00295"
                     /old_locus_tag="ABWED_B0016"
     CDS             complement(9679..9981)
                     /locus_tag="ABWED_RS00295"
                     /old_locus_tag="ABWED_B0016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_001140620.1"
                     /translation="MAKTLQELLASRSPESQARIQKMADELLLENQLHLIREELEISQK
                     ELAETLGIKQPSLSAIENRGNDLKISTMKKYVEAMGGKLRIDVELPTGKHIGFNV"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(9974..10330)
                     /locus_tag="ABWED_RS00300"
                     /old_locus_tag="ABWED_B0017"
                     /pseudo=""
     CDS             complement(9974..10330)
                     /locus_tag="ABWED_RS00300"
                     /old_locus_tag="ABWED_B0017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005194067.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            10969..11946
                     /locus_tag="ABWED_RS00305"
                     /old_locus_tag="ABWED_B0019"
     CDS             10969..11946
                     /locus_tag="ABWED_RS00305"
                     /old_locus_tag="ABWED_B0019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004761028.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="WP_004761028.1"
                     /translation="MDVLFSNFLDKPVWMWIFFILIVLVLLILDLGVFHRKVREIGVKE
                     SLALSAFYITLGLLFGAWVWWYLGPTAGINYVTGFVIEKSLAMDNIFVIAMIFSYFSIP
                     RIYQHRVLFWGIIGVIFLRAIMIGVGATLVSQFSWVLYIFAAFLIFTGIKMWLSVDKEY
                     EVGNSPILNFIKKRFRVTEQLHAEKFWVKKIDPKTNQLTWFMTPLFLALIMIEFVDLIF
                     AIDSVPAIFAITTDPFIVYTSNIFAILGLRALYFALSAMVDRFHYLKYALAVLLVFIGS
                     KIFIADGLGIAKIPPPVSLSITFIILFTGILYSLWKTKDQSTHR"
                     /name="TerC family protein"
     gene            complement(12151..12375)
                     /locus_tag="ABWED_RS00310"
                     /old_locus_tag="ABWED_B0021"
     CDS             complement(12151..12375)
                     /locus_tag="ABWED_RS00310"
                     /old_locus_tag="ABWED_B0021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005254632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="WP_004733276.1"
                     /translation="MNKLSLQGFLIPLFVSFFMSGINAILGALNEDKFLLSIVFSQWMI
                     NWIIAYPCLLFLVPFSKKIVLKLFYPQLK"
                     /name="DUF2798 domain-containing protein"
     gene            complement(12389..12721)
                     /locus_tag="ABWED_RS00315"
                     /old_locus_tag="ABWED_B0022"
     CDS             complement(12389..12721)
                     /locus_tag="ABWED_RS00315"
                     /old_locus_tag="ABWED_B0022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004733278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004282109.1"
                     /translation="MKFQFIFENFKALPNGTDAFKQLKILCEQEIGNNNNLDNSALFLI
                     YGFAKNYVLLYEDQEVTPEFAKKAKSQLIDYMSELNIALISQDSNLILETLNNISKKYF
                     KSSRIF"
                     /name="hypothetical protein"
     gene            12966..13577
                     /locus_tag="ABWED_RS00320"
                     /old_locus_tag="ABWED_B0023"
     CDS             12966..13577
                     /locus_tag="ABWED_RS00320"
                     /old_locus_tag="ABWED_B0023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004889724.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_010700378.1"
                     /translation="MIKMNTRIYLRASTKDQDAERALQILQDLNQNLNLGETIVYVENY
                     SGTKLDRPELNKLLSEANQGDTLLVESIDRLSRLTQQDFQELKRRIQEKGLRLIVADLP
                     TTYQMIQTSDSITHSILELINNMLIDLLATMARLDNEKRIERIKQGLERSGYKPTGKKA
                     NEAKHKRIKELLASGNMTKEEIAKAVNCGVATVYRVAKVI"
                     /name="recombinase family protein"
     gene            13603..13797
                     /locus_tag="ABWED_RS00325"
                     /old_locus_tag="ABWED_B0024"
     CDS             13603..13797
                     /locus_tag="ABWED_RS00325"
                     /old_locus_tag="ABWED_B0024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004733282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_005020922.1"
                     /translation="MSYLFTKEQGILKGQKTELMMKKHLIFLVFIVMVFILALVVKINN
                     DHKKGQALKKLELVEKQIH"
                     /name="hypothetical protein"
     Typing          complement(3281..4203)
                     /name="rep_cluster_1254"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.892"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(4745..6177)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.05"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gatataagac aaaagatgaa taccttagga attccgcgta aaaactacta atttcgtata
       61 accgccatta tgttaaatgg atagaaattg gtggaaaaat ctagttttgg aatttcgaat
      121 aagatatttc attttaagta caatttaata aattaattat tactctttaa taagaagatg
      181 aattaatata tcttacagaa atcaatataa gctattattt tatataaata tatgttgtta
      241 tcatatcaag ttaagtctga tcatcgtaag cctcaatgga aagcttctga aaaaagttta
      301 tggaaagttg aagaatgcat tgaactcgat atattttcct atggaattaa ctctaaatgg
      361 acaacgaata gtggagcaaa agccatagta tggtcttatc atagagattc tgattcagaa
      421 aaattagtaa aaataggcga agatcataga agatcgtctt ctattggtat tgtggactta
      481 ggattagcta aatttacttg tgatcataat aattgttggc atggatatcc tattgatcca
      541 gctactgata gtgttccatc atcaatttta aaaatatggc aaaacacttt aggaaaaaag
      601 cttgcaacaa aaattaatca agggaagcta aagctatgag ttttactaag gtctcattag
      661 aaggcgattt tttagaagca tttttatatt ctggaatact ctttacagtt gatacagatg
      721 gtattttatc aacttgtagt tttaaaaatt taataaatag ctatctagat aagactagaa
      781 ataaagactc aaaaattatt aagaaatata tacttaatga taataaaata aaccagtcct
      841 atgatggtga aaatataagt ctatatataa ctaaaaaatt tattgatgag aactgtgtat
      901 ttaatcaagt tccattagat gtatggccaa ctgatttaga cattaaaagt aatacagttt
      961 atatttcctc tgagagaggt ttagatattt taccatttca ttgggatatt aattcagtat
     1021 ccttttctga tgaaaaagaa aaacttaaga atatttggga tgatacaaaa gtatttaacc
     1081 ttgcaacagg gagttggggg agaacagttt tggctgcagg aaatgaggga gcaatagaaa
     1141 tatttaacgt ttatgatagt ttttataaaa atgtaattga tagtctttgt aaacccataa
     1201 aaccaaaaat tatttctaaa ggaacttggt tagaatgtga ttggggatat tctaaagtat
     1261 tagtgttaaa aaactttgat gagcaaaagg catttttgtt tgaaagtacc gctaaacaag
     1321 atgttttaga aaatagaaaa aaggaaaact ctgaaaattt tgaagaatcc gtagatgaat
     1381 tgataagaga tcttataaaa aaaccaaaaa atctagaaat taagaaagag aatatagttc
     1441 aatattctat tattaatgga aatatagttg gtatagatga taaaggaaat aaatataagt
     1501 ttaatttatc aaaaaataag tggcaattaa catcagagat caaatatgag tcatttctta
     1561 aagattattc agaaataaaa tttatttatc ctggaactat aattgaaagt aatgaaaaaa
     1621 tttacattga taaaagaaat catctctacc aattagatga taattttatt aaatggagaa
     1681 cttttcctcg ttcaaaaaac tatgtaaacc atactcttat catcaaagat aatgaattag
     1741 atatttatgc tctttaagtt tttctaaaat taacataata cacgttatgc gaaatttaaa
     1801 aaatgagatt atcattttga taattaagga aattttaaaa tttcgtataa tcgccattat
     1861 attaaatgga taatgaatta gtggaaaaac ccagttttta tacttgaatt aaaagtagtt
     1921 gaatcatact ggatccccta taatttatta aagttcctga taatcatcgt tatgaaaaaa
     1981 ataattttga ctccaataat cgctctctta ttagggctca cagggtgttc tactcaacaa
     2041 gttaaaccaa tcccctataa tcaaaaagct cttaatttga aagtggggat gactaaagat
     2101 gaggttataa accttttggg tacacctaaa aaagtatctg ctatcaagac aaatgaagga
     2161 ttggaggaag tctattcata ctgggggata tcacgattag tttatagtta tactccaatt
     2221 gataacgaag ccttatctca agatcgttta gctgttactt tcttagatgg aaaggttacc
     2281 cagtggggag ataaatttga tccgactgta atatctaatc aaacattgaa aacgattcaa
     2341 caaacttctc aacaatatca acaaatgtat gaaaagcaga tccagaatac tagagagaat
     2401 aaataaattt attggtaaaa ttctttaaaa ttaacataat acaccttata cgaaatgcgt
     2461 tataattctc ttgaaaattc aacgctgtag ttcctagttg atagcccgaa catccctgtt
     2521 cgggcttttt ttatcttttt tagtgttcca cgcttaataa ataagcaatg ttccacgatt
     2581 ttattcagtt ttaataaaca tcatccggtt ttttggtgag tcattatttc atgtgcttgc
     2641 actgaaattg gctcatcaaa aaagtggagt cctggttaac cgtaaggaag gaaaaaacgg
     2701 ctcaggaagc ctctcttttt aggtgtaggc tcaatatgtt ctggaacagg gatgcgtttg
     2761 ttctctggag tagtcaatcc gtcatagttc gattcagtct gtggccgaga atctgttgct
     2821 atatcctgtt cagatttatg atcggtaaac gtagtcatat tggcttttgg agcttccagt
     2881 aggcgctgca tggcctcaat ctgttcttga taaaacgatt cacgttctaa tgattgattt
     2941 tctctctgta ttgcctgatt taattgtctt tcaagcatgt caacttgacg ttttagcgag
     3001 tcaacttctg ttaagttatg cctactaatt gattgacttt ggttgacact agaattgctt
     3061 ttttgtggtt ccccaaaaac tctcaaggct tctgaaaagt caattaatcc atccgatcct
     3121 tttgataaat ttcctttctt tatatgggca tatacagctt gtcttgaata tccatataat
     3181 ttcgctagtt ctgaaactga cagttttttc attatgtaaa ccagttttca aatagtgtta
     3241 atagttgatt gttaacttta ctatgtcaat tgacatcatt cattgaaagc cgacttttcg
     3301 aagtaatggc ataaattctt ttaatttatt tggctcttgt agcatggtta caatccgagc
     3361 agcaaactgt tcatagcttt cattaccttg tgaaagttta gccatctcag gaagttctga
     3421 taatttacta gcaaataaat atcgttgtgc atcagtcaaa ttgattttta gctctttgtc
     3481 ctgactaatg tcattggctg tatttgttgc tgactttttc tgtttgaagc taaatgaaaa
     3541 gcccgtaatg gatctgccgg ttttatgttg ttcaactttg acattaatat cggtatgttc
     3601 atttacttgc tttaatgcaa tgtctaaaac atatttctta aaatcgtaca tccttttgta
     3661 ttcagtctcg agtacaccta ttttttgtct gaaatcgtag atagttataa gaggcgtttt
     3721 tccggtacta cgccacgcaa tcaatatttc atataagcga acagcataag cactcgttaa
     3781 attgcttatt tgctgtatct catactttgt aaattgttct tctaatcgag ttattaaagg
     3841 cacaatagca ggagcaaaaa taagtctaac gacagcctca ttatcaatat aagcaacctc
     3901 gcttacccat cttgacttgt gattaatgac attacctttt tcactaagac tttgataact
     3961 gaattgtctt gcaaataggt catcgcaagc atcttttaag gcttgataag cagtatttcg
     4021 atgtacacca aattgattga tataactttc agcatgaact gttaatggat cattagcatt
     4081 tatcccttta cctgattccc tggcctcaac gatagctaaa agaattaatc gttgttctac
     4141 tagatcaagg ttataactag cattaattaa agcgttatct ttgacaatta gttcggtttt
     4201 catatgaaaa atttatattt ttaagtcaat aagacgaggt tatattatca actcgcttaa
     4261 tgcaaggtaa acctcgtttt aatgcaaggt aaacctcgtt ttaatgcaag gtaaacctcg
     4321 ttttaatgca aggtaaacct cgttttataa tctctcaaag ccttataaat tcaggtttac
     4381 aggctatcta aaagcattta aaagcttaaa aataattaaa atcccaggga atgaaaaatt
     4441 gcccttggtt ttcgctttgc tcaaactcta ttgaatctcg ctttcgctcg attcgtcggg
     4501 gcaatttttg gttcatattt tcgtgaattt aagaaaaagc aaaagcaaga tcaaaagcag
     4561 ctcggaattc gcttcgctca tgagcttttt ttctcgctac gctcgattta ggaatcaaac
     4621 ttggaaagtt cgcacccatg cgggatgctc tgtagggtag gtgattaatg gatagggagc
     4681 ttgtaatcag ggaaagggat gggcaaattt tctcagttcg ctcgtagaca ctcgctctgt
     4741 ttaacgactc atgccacgag atttcatttt aggcgcatgt tgctgtgctt tgagttgttg
     4801 ttcttgtctg agtttcatct gttgttctgc tttaatctca tccacacatt ttttaacgat
     4861 cttgtaggac tggtagaggg tctggtattg cttggctttc tccggatgct gtttttgata
     4921 tttcttccag gcatgttcct tgaaagcatc attttgcagt aattccttca caccatgttt
     4981 tttctggtgt tcatgctgac ctttcagctt tttgtgttcc tgataaatct cgtcacgttg
     5041 ggcttcccag gctttttttc caaacagtaa gggtttgttc tgggttaatt cgttgtgttg
     5101 atcgaccagg ctttgtaatt tgtcatgact ttgcttgagt ccgcttttca cgatctcctg
     5161 ggcgagctgc tgattgaact cgttttgatg tttgtgttgc ttgctcaggg tcttttcggc
     5221 cttctctaga tgctgctgca tgaaatccag gtctagattc gcgtcttttg cgctgatttc
     5281 gcgttttaat actttgccaa gggtttccct ctgttttttc tcaaacaggg ctttttcatc
     5341 aattggtggc gttttagcgc tatttttgat ttggatctgc tggtctgttt tcagtgtgct
     5401 gagtttgatc tcattttcag caatcagcag atccatattt ttgccgtatt gcaactgagc
     5461 ctgatttctc tgtttgattt cgtcattgtt tcggctgatt tcggtttcga tgcctcttct
     5521 acgtagttcg gtgactttcg gtccttcgtg cagggtggct tccaggccat tctcctggtc
     5581 tttgtagctg cggtggtcga cccgggcggt ggacccggcc agatccaggt gcatattgca
     5641 gatatcggca aagtgttcgc gccagtgttc aatttctcca ctgtgtttct gatccagttc
     5701 ccgggttttc tcggtaaaac cttccggagt gagcttgcgt gtgctcatca ggatatgggc
     5761 gtgataattg cgttcatcac tgccggatcc agtatgcggt gcatggatac aggcatcgac
     5821 ggccacctga tgtctgtcca cgatactggt gcatagttct tcgagcatgg ccagacgttg
     5881 ttcctggttc aactcacttg gaaaggcaat ttcaaattcc ctggcgactg tcgagttttt
     5941 tcgggtttcc cgttgttcca cttcgttcca gagtttttcc cggtttttta aatgctttgg
     6001 tgcgccttcg ggtaaataaa tttctttgta ttccacacct gtttttcggc tgtagtcatg
     6061 ttctcggcct tcacgttcac aatagatctt ttcacctgcc cgataggcga ttgcagcagt
     6121 ggcggaacgc cctgcagatc gtgctacggt tttgactgaa aagtggtaaa tcgccatgcc
     6181 tgtttttcgc ttttaaattt tcacatagtg aaatacaacc gtgcatacgg ttgtcggggt
     6241 attgggggag accccaatcg gacttgcaaa cgaagtttgc ttaagtgcgc tcttcgagga
     6301 aaacattact atacatatag caccttgaat gcatattcaa gtgcgcttat gttagggatg
     6361 gtttcccagg atgatggtat tggattaatg agtattactg aaacactaga cagcaagatt
     6421 aaggcacagg aagaaaagct aaaacagtta aaggctcaaa gacaggcagc actagcaaga
     6481 gaacgtgcta aagagaaaca acagactcgt aaagatgata ctagacgcaa aattctaatc
     6541 ggatcctgta tgctaaaaat cactgaagaa gatgaacagg cacgggctaa attgatcgct
     6601 cagatggacc ggtatttgac ggatgaaaga gacagaaagt tatttaatct ttaatgtaga
     6661 actctaatat gaataattta tcaataactt ctcccaaggc tcccttgatt aaatcttggt
     6721 ttggttttgt cttaggtgca gcatctgttg ccataagtct atttggctta tccaagagtt
     6781 ttgatttagc cggaatttta agtactttgg gttttttatg tttttggtat ccttggagtc
     6841 aaatgactgg ttggaaggat ccaataaagc actttttcaa agtagatgat caatcaatga
     6901 gtaaaccatg catctactta acattgatcg ccacaatact acttataact tctaccgtga
     6961 taacttttat taattaaaat tctaggaaat atccattcat ttggctttat acaaagtgag
     7021 gtttttactt cacttttttg attgcgtata agagatttta tgttaatcag atgatcaaaa
     7081 ggcccgtgaa tagggccttt cgtataaggt gtattatgtt aattttagaa aaaacttaaa
     7141 attatctatt aaagaatcat atatttttgg attacttata agtgaaatag cacctatata
     7201 gtgcaggctt agctagaaaa actataacac tctaagaaag tatgggtttt tatgtacaag
     7261 gcaatttcat aagaagccag atgataaaat tcgaggctcc ccaattttgt aaatcaaata
     7321 tatgcaaaat aatgtgagta agccggcagt accagcatta acactggctg cactaggagt
     7381 agtattcggc gatataggaa cgagtccatt atatgcatta aaagaaagct ttcatgcgac
     7441 acgcgggcta cctattaatg aacttaatat cctgggaatt ctctctttaa tcttttggac
     7501 cattacctta attgtttcgc ttaaatatgt tttggtgatc atgagagctg ataacaatgg
     7561 tgaagggggt atcatggcat tactcgcctt aaaccttagg caaaaaggat tgtcccgtgg
     7621 aaaaagactg gcaatcatta tcttaggttt cattggagca tccttattct tcggtgacgg
     7681 tatcatcacc cctgctattt ctgttttatc tgcagttgag ggactttctg ttgctgcccc
     7741 gtcatttgat cgcttcatta ttccaatcag tattggaatt cttatagctc tctttgccgt
     7801 tcaaaaacat gggactgcaa tgatggggaa gttttttggt cctattaccc tgttatggtt
     7861 cctatctatt ggtgccattg gcattatgag tattattcaa agtccatttg ttcttgcatt
     7921 attcagccca cactgggctt ttttattcgt tattaacaat cctcttattt cattttttgt
     7981 aatgggagct gtggttttaa cagtaacagg tggtgaagcc ttatatgcgg atatgggaca
     8041 ttttggtatt ttaccgatca agttaggctg gtttttaatt gtactgccat gtctgatgct
     8101 gaattatgct ggacaaggag cgttactgtt aagagatcct tcggctatca ccaatccttt
     8161 ttacctgctt ctgccttcag cccttctata tcctatgatt attctggcta ctgcagctgc
     8221 agtgattgcc tcacaggctt taatcagtgg ggtattctct atggctaaac aggcaataca
     8281 gttgggatat ctacctaggc ttacagtttt acatacctcc gcttcggaaa ttggacaaat
     8341 ttatattcca ttactgaact ggctcctgtt tatctctatc accatcttga tcttaatatt
     8401 tgaaagtagc agcaagttgg caggtgctta tggtctggcc gtcactgtga ccatgttctg
     8461 tgacacactg ttagtagctt ttctggctta tagttactgg aaatggaaaa catggaaagt
     8521 cttactgttc attattccat tcgcctttat tgatctggtt ttgctgagtt caaacctatt
     8581 aaaagtcctg attggaggct gggtaccagt cgttattgcg gttattgttt ttaccctcat
     8641 gatgacctgg aaaaaaggca gagagattct tcaggacaaa ctgcagaagg acacgcttcc
     8701 actgaatgtg tttcttgagc accttgaaca gacaggtcag aaagtgagtg gaaatgctgt
     8761 atttttaact ggaacgcctc aagtcgtacc ccatgcgctt ttacataatc taaagcataa
     8821 taaagttctg catgaacgta attttttggt gacaatcaag acttctgaaa tcccgtatgt
     8881 tgatgaagct aagcgaatcg ttactgaagt actagagaat ggttttttca gaataacgat
     8941 ccattatgga tttaaagaag agcctaatgt gcctcattca ttgaaacaag ctttttcagt
     9001 actggatctt gagtatgact taatgaatat cagcttcttt gtttctagag aaagattaat
     9061 tccaagtctg tccagcaaga tgtctacatg gcgtgaaaag ttatttgtcg caatgcaaaa
     9121 aaataccagc cctgtcagtg atttttataa aatcccttct aaccgggtgg tagagctagg
     9181 tagtcagatt gaaatttaag aatttctaaa attaataggc acctttaaat aagggtgcct
     9241 atctgatcag aataaatctt ttactttaat tttccaatag ttgaccaatc tctaaagctg
     9301 aataaccacg ttctttaaag atcaaaataa ttgctttttt ataatcttca gaagtagagg
     9361 tcttatattt tgttataagt tgaatttcag aattaagctg aaggtcattt tttatcgtca
     9421 acacttccgt agagacttta ttaaaaatct tgaaattaaa catacaggcg ctctttatca
     9481 atattatagc ggcccgacta caacaaatac ctgccttagc tcagctagac acccttggct
     9541 gcaaggatta caaattagac aagattctgc aagttttgct ccattgaaag tatgaatatt
     9601 caactaaaga aggttccatt taacataatg gttgttatac gcaataaaaa tgggagccta
     9661 tagctcccat ttttattgtt aaacgttgaa tcctatatgt tttcctgttg gaagctctac
     9721 atcaatacgg agcttcccac ccattgcctc aacatacttt ttcatggttg aaatcttaag
     9781 atcattgcca cgattttcta ttgctgaaag cgatggctgt tttattccca aagtttcagc
     9841 aagctctttt tgagaaattt cgagttcttc acgaataaga tgaagctgat tttctagcag
     9901 taattcatca gccatttttt gaatacgggc ttggctctct ggagagcggc tagctaacaa
     9961 ttcttgcaga gtcttagcca ttagatttat ctcctagggt agtgagatga agttggtatt
    10021 gttcatctgc aatatccagc atgtctttat aaaaacgctt tttaccgcct ttatcagcaa
    10081 tacatagaac aatagcctgt cgtaagggat caaatgcgaa gaaagctctt aagggtctac
    10141 cgcgatgttg aacacgcagt tctttcatat tggtaaattt agactcatac acagtatcta
    10201 ctaaaggacg gcctaaactc ggtccctact gctgtagaac aaccagggca gctaggacct
    10261 tctcttgtgt aggttcatct tgctgttcaa gccactgatt aaatagatct gtcgtaatga
    10321 ctgtccacat accacaacca aaatatagat tatagtctat attttagggg cattatgttt
    10381 gttttgcaaa tgagaaagga taggcaataa aaaagcccga catcctgtcg ggcttttcca
    10441 tatttggcgc ttagatctga ctcctgtctg atctcacgtt ccttgttgtt catcttattc
    10501 tttttatctg gagcatcctg ctctctatgt cttcatgata ggaaatttcc agtattgcgg
    10561 atagcagcaa agggccgaga ttggtatgag ccagggctta caaaatagcc taaattttat
    10621 ccacaaaaaa gtgtggataa ttttggacat tgaggcattg ataaaatttg aaaaacctca
    10681 aaactgacta ttttttcagt caaatttatt ggcaagaagt atcaaaaccg ctcataaaaa
    10741 agccgacttg tttcaaaatc ggatttttgg gcattcttgg atttttctat ttttattaag
    10801 tgtttgatat ttatattaat atacaagtgt atttcgtata acgcccatta tgttaaatgg
    10861 gggatttatg aattctgaat atatctttat atgtaataaa tattgacaag tattattgat
    10921 aatgagcata tcttctcata taagcacaag tataagcaga ggaaaactat ggacgttcta
    10981 ttttcgaatt ttttagataa acctgtttgg atgtggattt tctttatcct tattgtactt
    11041 gtcctgctta ttctagatct tggagtattt catcgtaagg taagagagat tggtgttaaa
    11101 gaaagcttag cactatcagc attttatatt acattaggtt tacttttcgg cgcatgggtg
    11161 tggtggtatc tcggacccac ggcagggatt aactatgtaa ccggttttgt aattgaaaaa
    11221 tcattagcga tggataatat ctttgtcatt gcaatgattt tttcatattt ctcgatacca
    11281 agaatttatc agcatagagt cttattttgg ggaattatag gtgtaatttt tctaagagct
    11341 atcatgattg gcgtgggcgc aacactggtc agccaatttt catgggttct ttatatattt
    11401 gcagcctttc ttatttttac agggataaaa atgtggctaa gtgttgataa agaatatgaa
    11461 gtcggaaatt ctcctatttt gaactttatt aaaaaaagat ttcgtgtcac tgagcagctt
    11521 catgcagaga agttctgggt gaaaaagatt gatccaaaaa ctaaccaatt aacctggttt
    11581 atgacaccac tttttctagc cttgatcatg attgaatttg tagatctgat ctttgcaata
    11641 gatagtgttc ctgcgatttt tgcgattacg acagatccat ttattgttta tacttcaaat
    11701 attttcgcaa ttttgggatt acgtgcttta tattttgccc tatcagcaat ggtcgaccgc
    11761 ttccattatt tgaagtacgc attagcagta ttgttagtct ttattggatc aaaaattttt
    11821 atagctgatg gattaggtat agcaaaaatt ccaccgccag tctccctttc gatcacgttc
    11881 atcatcttgt ttacaggaat tctatactct ctttggaaaa caaaagatca aagtactcat
    11941 aggtaaaaat tacaatcctt taaataatta atagctacca ataaaacagt ggctgttaat
    12001 tattctgatt ataagttttc ctaaaattaa cataatacac gttatacgaa atgcccttga
    12061 tcaagggcat ttttagccgc tacttaacgt agaatatctt atgcaaagtg aaaaacgacg
    12121 gtctattgtc ctcgtttcat ataacattca ttatttcaac tgaggataaa aaagctttaa
    12181 aacgatcttt tttgagaaag gaacaagaaa caaaagacat ggataagcaa taatccaatt
    12241 aatcatccat tgtgaaaaaa caattgataa taaaaattta tcttcattta aagctcctag
    12301 aatagcgtta atgccactca taaagaatga gacaaaaagg ggaatcaaaa acccctgtaa
    12361 tgatagcttg ttcattgatt attaagattt aaaatattct tgaactttta aaatattttt
    12421 ttgaaatatt attaagagtt tctaatataa gattagaatc ttgactgatt agagcaatat
    12481 taagttcaga catataatca attaattggc tttttgcttt ttttgcaaat tcaggagtaa
    12541 cttcttgatc ttcgtacaat aagacgtagt tcttcgcaaa cccataaata agaaataagg
    12601 ccgaattatc taaattatta ttgttgccaa tttcttgctc acataaaatt tttaactgct
    12661 tgaaagcatc tgttccattt ggtaaagctt taaagttttc aaatataaat tggaatttca
    12721 ttttggggac tcacttaaaa agaaactcta cagctttagt cactcccaat caatatctaa
    12781 tttttccaaa attaatatga tacgtattat acgaactccc aaagcatggc tttcagtcat
    12841 gctttttcta tcaaaagagt atagctgaat gataacgagc caaataacat aaaatatcta
    12901 tcattaaact taatgcaata attaaatgat agtgttatca ttaatctaat agaattaaag
    12961 attaaatgat aaaaatgaat acacgtattt atctacgagc ttcaactaaa gatcaagatg
    13021 cagaaagggc tttgcagatt cttcaggatc taaaccaaaa cttgaatttg ggcgaaacca
    13081 ttgtgtacgt ggaaaactat agtggtacga agttagaccg acctgaattg aataagctac
    13141 tgtcagaagc aaatcaaggt gacaccttgt tggttgaaag tattgaccga ttatcacgcc
    13201 taacccaaca ggattttcaa gagctgaagc gcaggattca ggagaaagga cttcgattaa
    13261 ttgtggcaga tcttcctact acctaccaaa tgattcaaac cagtgacagc attactcatt
    13321 caatcttgga actcatcaac aatatgttga ttgatctcct ggcaacaatg gcccgtctag
    13381 acaatgagaa acgtatagaa cgtattaagc agggcctgga acgttcgggt tacaagccaa
    13441 caggtaagaa agccaatgag gctaagcaca aacgaataaa agaattgcta gcatctggca
    13501 atatgactaa ggaagaaatt gccaaagcag tgaattgtgg agttgcaact gtctatcgag
    13561 ttgctaaagt catttaaaaa ggcttgtatc agtgcttccc ttatgtcata tctatttaca
    13621 aaggagcaag gcattcttaa aggtcaaaag acggaattaa tgatgaaaaa gcatttgatt
    13681 ttccttgttt ttatagtgat ggtttttatt ttagctctag ttgtaaagat taataatgat
    13741 cataaaaaag ggcaagcatt aaagaaattg gaactagttg aaaagcaaat ccattaggta
    13801 aaaattgact tatacttatt taaataacgc taataactaa gcccaaaaaa cacctagacc
    13861 cagtgctata cacgcttagt tttttatatt tcaaaagagt attttagcaa atatcataaa
    13921 agaccggtct attctcaagc cggtttttta tatcgcagag tatcatggct tcatcataga
    13981 tatatgaata ttaagataaa taaaaagccc tatagtctct cccgttacta taaggcttat
    14041 aaaacgctgt aattttttct taattaccta cttttcgatg taggttatga atatatttta
    14101 cctattggta aaaataaa
//
