LOCUS       NZ_CP032111             5223 bp    DNA     circular CON 31-MAR-2024
DEFINITION  Acinetobacter lwoffii strain EK30A plasmid pALWEK1.9, complete
            sequence.
ACCESSION   NZ_CP032111
VERSION     NZ_CP032111.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN05251105
            Assembly: GCF_019787625.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter lwoffii
  ORGANISM  Acinetobacter lwoffii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter.
REFERENCE   1  (bases 1 to 5223)
  AUTHORS   Mindlin,S., Petrenko,A., Kurakov,A., Beletsky,A., Mardanov,A. and
            Petrova,M.
  TITLE     Resistance of ancient and modern Acinetobacter lwoffii strains to
            heavy metals and arsenic revealed by genome analysis
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5223)
  AUTHORS   Beletsky,A., Kurakov,A., Petrova,M. and Mardanov,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2018) Lab of molecular cloning, Institute of
            Bioengineering, Research Center of Biotechnology of RAS, Prosp.
            60-let Oktyabrya, bld 7-1, Moscow 117312, Russia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Newbler v. 3.0
            Expected Final Version            :: yes
            Genome Coverage                   :: 25.0x
            Sequencing Technology             :: 454
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_019787625.1-RS_2024_03_31
            Annotation Date                   :: 03/31/2024 06:12:30
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,448
            CDSs (total)                      :: 3,336
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 112
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 222
            CDSs (without protein)            :: 222
            Pseudo Genes (ambiguous residues) :: 0 of 222
            Pseudo Genes (frameshifted)       :: 80 of 222
            Pseudo Genes (incomplete)         :: 138 of 222
            Pseudo Genes (internal stop)      :: 46 of 222
            Pseudo Genes (multiple problems)  :: 39 of 222
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP032111.1.
            Bacteria and source DNA available from Dr. Petrova M.A.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5223
                     /organism="Acinetobacter lwoffii"
                     /mol_type="genomic DNA"
                     /strain="EK30A"
                     /isolation_source="permafrost, Kolyma lowland"
                     /db_xref="taxon:28090"
                     /plasmid="pALWEK1.9"
                     /geo_loc_name="Russia"
     gene            complement(223..543)
                     /locus_tag="ABEKA_RS01460"
                     /old_locus_tag="ABWEK_H0001"
     CDS             complement(223..543)
                     /locus_tag="ABEKA_RS01460"
                     /old_locus_tag="ABWEK_H0001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005407434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_039902758.1"
                     /translation="MFKILTASLLGLLLIGCGNSDSTGSPKPKKPKTETEIQGAKFSSM
                     SSCLKSIQSKTQLPLKPMTDKPDHVSGFLGDTDRQFNCEVKQTGTEGTFVEGWFTVEVE
                     VY"
                     /name="hypothetical protein"
     gene            574..909
                     /locus_tag="ABEKA_RS01465"
                     /old_locus_tag="ABWEK_H0002"
     CDS             574..909
                     /locus_tag="ABEKA_RS01465"
                     /old_locus_tag="ABWEK_H0002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_171770288.1"
                     /translation="MKKLIFIVITLLTTNHVLADTLIPRSAAGDKGKYYLIKKTKSGDI
                     ISAVHKRVGVDSIGYTSTKTNCKTGKMQEIGYSEVSAQAIKASPTKWFDLVPGSSKHDL
                     YKFLCKK"
                     /name="hypothetical protein"
     gene            complement(916..2412)
                     /locus_tag="ABEKA_RS17125"
                     /old_locus_tag="ABWEK_H0003"
     CDS             complement(916..2412)
                     /locus_tag="ABEKA_RS17125"
                     /old_locus_tag="ABWEK_H0003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004282282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_159373234.1"
                     /translation="MIIKIHSRGSGSGSGPVDYLLGKYRDREEARLDRGDPEQMVQLID
                     STHFAQKYTSGVLSFAERDLEEHQKQKIMDSFERTLLPGLDKDQYSILWVQHLDKDRLE
                     LNFVVANVELQSGKRLQPYYDKADRPRLNAWKDLVNDYYKLHDPNDPLNKRELCTPNNL
                     PKAKEAAAHAITDGLLNIAESGNLKNRDDVISMLENAGFEIARKTPKSISIKDPEGGRN
                     IRLKGKIYEQDFRFGQELRADIEAASARYRADRGERIEAARKTLNRLTERKRESHEYRY
                     PRAKLTDTPDYIKKLDDRINRHRYSDSPSMGRSDISELQNKQEFRANYPTESSYPRTQG
                     QRGERAISGLYRQEEPQATMRISEQGSRNLEERQFYGSDGVLSDDRIRKAIARSTGTDA
                     GTAAGERRSFTADVREYTARKSGYQGSLQDLTGQYFRSRSEASGKLEQLNKACQRLEWT
                     TERLRETKQAFEQSVKHNTRAVTQVEKMNEPQRSYGLRMR"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(2393..2737)
                     /locus_tag="ABEKA_RS01480"
                     /old_locus_tag="ABWEK_H0004"
     CDS             complement(2393..2737)
                     /locus_tag="ABEKA_RS01480"
                     /old_locus_tag="ABWEK_H0004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011178376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_004282283.1"
                     /translation="MADKELIKRTKEIKIRLTEEEHQALLNRCTKASLATWMRETCLGE
                     KQTKQSKVIEVDPKLLRQLAGIGNNLNQIARLVNQHSKQDSVLDRIAIITALSSIEREL
                     QRLNDDHKNT"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            complement(2987..3340)
                     /locus_tag="ABEKA_RS01485"
                     /old_locus_tag="ABWEK_H0005"
     CDS             complement(2987..3340)
                     /locus_tag="ABEKA_RS01485"
                     /old_locus_tag="ABWEK_H0005"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_082226415.1"
                     /translation="MENNKDKDLDDLYLELLGYYASLSVISILNFYTLNVLINNENVPW
                     YIKIIIGIVALALFGFSGAIGGALFNKVWVPMQLISETQNDFLAQVSVKIVIILYYAIL
                     IIPPLYTLNNLFN"
                     /name="hypothetical protein"
     gene            complement(3442..4305)
                     /locus_tag="ABEKA_RS01490"
                     /old_locus_tag="ABWEK_H0006"
     CDS             complement(3442..4305)
                     /locus_tag="ABEKA_RS01490"
                     /old_locus_tag="ABWEK_H0006"
                     /inference="COORDINATES: protein motif:HMM:NF041770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CFI-box-CTERM domain-containing protein"
                     /protein_id="WP_159373230.1"
                     /translation="MDHKKLVGNLFGVDIHLPLKRNEVRDLVRARQVFIEDLIQQKNLN
                     ERQASLDASDLLIEFCTQLNEEEAKNLSELLKEEAQALLPKIEDTLQDIESSSKTQLEA
                     DLSHIEAQAIFNELSANLQVYGTNSKLSGANPANIDLALAHINRSLELQPDNPIFLNLK
                     GLLLWQGKNDRVGALPLIQRAAKLDPKNINIQHNLKAIEDPKGCFIATASFGTPVAYEI
                     NELRYWRDTKLSGSRFGRLFINTYYTLSPPIAKLIAKNAFMKKVIRSLLRPLISYVKKT
                     NQNDSR"
                     /name="CFI-box-CTERM domain-containing protein"
     gene            complement(4333..5223)
                     /locus_tag="ABEKA_RS01495"
                     /old_locus_tag="ABWEK_H0007"
     CDS             complement(4333..5223)
                     /locus_tag="ABEKA_RS01495"
                     /old_locus_tag="ABWEK_H0007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011512341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_159373232.1"
                     /translation="MRDDQFLADFYKKIPHKPYCTDDLGYSFINPKQIAIKKRYLQHNP
                     PCKIVYLVFDLDRPDGVMAWFDAGLPKPTWTSQNPKNGHAHIGYELKAPVSTTTASKQK
                     IIDYLAVIEAGMARQMGADSGYSGLLTKNPCHTYWRTTIWTREAYELNYLADFVDLKPL
                     TQKEQGTGLGRNCTLFDIARKWAYKAIREYRGSTFDSWLGAVLKECLSVNSAFLEPLPY
                     SEIKATARSIAKYCWKKDAYCYQEFIDRQSRKGQLGGKVSKRPKLENSERTTQPWLELG
                     ISQSTYYRKKKLGKL"
                     /name="replication initiation protein"
     Typing          complement(920..2415)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gaaatgcaac gtcctatgtt gcaaatgctc aaaacatcag taaaatacaa gtaacgattt
       61 tgcgtttaat gatgctttaa gtatttgtta aacaaagaaa agaaagtagg tttgccgacc
      121 tgcttttttt tcgcctaatg aaatgtatca ttgtttataa tacatttcaa aatttctttt
      181 cataacattg acttatgcca tatttggcta gtctattatt ttttaataaa cttctacttc
      241 tacagtaaac caaccttcaa caaatgtgcc ttctgtgccg gtttgtttaa cttcgcagtt
      301 aaattgtctg tctgtatctc ccaaaaaacc tgatacatga tcaggcttat ctgtcatagg
      361 ttttaacggt aattgggttt tgctttgaat cgactttaaa cagctagaca tactagaaaa
      421 tttagctcct tgaatttctg tttctgtctt aggcttttta ggttttggtg agcctgtact
      481 atctgaattt ccgcatccaa ttaacaataa accgagtaaa ctagcagtaa gaattttaaa
      541 catttgatgc ccctcattag agttattaaa aatatgaaaa aattgatatt tatagttatt
      601 accctgttaa ccactaatca tgttttagca gatactctaa ttcctagaag tgctgcaggc
      661 gataaaggta aatactattt aatcaaaaaa acaaaatcag gcgatattat ttctgctgta
      721 cataaaaggg taggcgtaga tagtattggt tatacatcta ccaaaaccaa ctgtaaaacc
      781 ggaaaaatgc aagagattgg atatagtgaa gtatctgccc aagctattaa agcaagtcct
      841 actaagtggt ttgatttagt acccggttct agtaaacatg acctatataa atttttgtgt
      901 aaaaaataag tctacttatc tcattcgaag gccatagctt cgctgtggct cattcatctt
      961 ttcaacttga gtaactgctc ttgtattgtg cttaactgat tgttcaaaag cctgttttgt
     1021 ctctcgaagt ctttcagttg tccactcaag tcgctgacat gctttgttaa gttgctccag
     1081 ttttccgctt gcttctgatc tgctgcggaa atattgtcca gtaaggtctt gaaggcttcc
     1141 ctgatagcct gattttcttg ctgtgtactc tcgaacatct gccgtaaagc ttcttctttc
     1201 tcctgctgct gttcctgcat ctgttccagt gctgcgagca attgcttttc taattctgtc
     1261 atcacttaat accccatcgg aaccataaaa ctgccgttct tccaagttcc tgcttccttg
     1321 ttcactaata cgcatagtcg cttgcggttc ttcttgtcga tacaatcctg aaattgcacg
     1381 ttcgcccctt tgtccttgag ttcttggata gctagattct gttgggtaat tagctcgaaa
     1441 ttcttgttta ttctgtagct ctgatatgtc agaacgcccc atgctagggc tatcgctgta
     1501 acgatggcga ttgatacgat catccagctt tttaatataa tcgggcgtat ccgtgagttt
     1561 tgctctcgga tagcggtact catggctttc tcgttttcgt tcagtaagtc gattaagcgt
     1621 ttttcgtgct gcttcaatgc gttcgccacg atctgctctg tatcttgcgc ttgctgcttc
     1681 gatgtctgct cgaagttctt ggccaaatct aaaatcttgc tcatatattt tgcctttcaa
     1741 ccgaatgttt ctaccgcctt cggggtcttt gatactgata ctttttggtg ttttacgagc
     1801 aatctcaaaa cctgcattct caagcatact aatcacatca tcacgatttt taagattgcc
     1861 actctcagca atatttaaaa ggccgtcagt aatcgcatga gctgccgctt ctttagcttt
     1921 gggaaggtta tttggagtac ataactcgcg cttatttaaa gggtcatttg ggtcatgtaa
     1981 tttatagtaa tcatttacaa ggtctttcca tgcgttaagc cttggtctat ctgctttgtc
     2041 ataatagggt tgtaaacgct tgccactttg taactcaaca ttggctacaa cgaaattaag
     2101 ctctaaacgg tctttgtcca ggtgttgtac ccacaaaatc gaatattgat ctttatctag
     2161 tcctggtaat aaagttcgtt caaaactatc cattattttt tgtttttggt gttcctctaa
     2221 atcacgttca gcaaaagaca aaacacctga agtatatttc tgagcaaagt gggtgctatc
     2281 aatcaattga accatttgtt caggatcacc acgatctaaa cgagcttctt cacgatctcg
     2341 atattttcca agcaagtagt ccacgggacc gctccctgaa cctgaaccac gactatgtat
     2401 ttttatgatc atcattcaat ctctgcaatt cacgctcaat actcgataaa gctgtaatta
     2461 tggcaattcg atctaatact gaatcttgct tactatgctg attcactaat ctggctatct
     2521 gatttaggtt gtttccgatt cccgccagtt gtctaagtag ctttggatca acttcaataa
     2581 ccttgctttg ttttgtctgc ttttccccta aacaagtttc acgcatccaa gtagctaaac
     2641 tggctttagt acatcggttt aataaggctt gatgttcttc ctctgttaaa cggatcttta
     2701 tttctttagt cctttttatg agttctttat ctgccattgg atctctcgtt tggtaacacc
     2761 aaaataggaa aattttaatt tttctgtttt ggtgtttttg ggggtatcgg gggcaaagcc
     2821 cctgatccag tatcacaaaa gggaatcttc gagcgaagcg aagaaagtcc cctttgtgta
     2881 tgctggctta ttgtaagaaa agtaccagta atttgagttg aattcaactt tacttattta
     2941 aatgacatcc ggtaagtagc ttctaatagg atttagaaaa ctatctctaa ttaaatagat
     3001 tattgagggt ataaagggga ggtataatta aaattgcata ataaagaata attacgattt
     3061 taacgcttac ttgtgctaag aaatcatttt gagtttctga tatgagctgc ataggaaccc
     3121 aaaccttatt aaataacgcc ccaccaatag cgccagaaaa tccaaataaa gctaaagcta
     3181 caatccctat aattatcttt atataccaag gaacgttttc attatttatt agaacattta
     3241 gagtataaaa attcaggata ctaataacac ttaaactagc gtaataacct agaagctcta
     3301 ggtataaatc atcaagatct ttatctttat tattttccat ttttactctg ttgataaatt
     3361 agaaggaacc taaagagagt tatctaattt aggttcactt agccatttcc taagataaaa
     3421 acgctgttat ctgatttagg cttatctgct gtcattttga tttgttttct ttacataact
     3481 tataagtggt cttaagaggg atctaatgac ctttttcatg aaagcgtttt tagctatcaa
     3541 ctttgctata ggtgggctta gcgtgtaata ggtgttgata aataaccttc caaacctact
     3601 tcctgaaagc ttagtatctc tccaatatcg taattcatta atttcgtaag ctactggtgt
     3661 accgaatgaa gctgttgcta taaaacaccc tttaggatct tcaatagctt ttaaattatg
     3721 ttggatattg atgtttttgg gatctagttt tgctgctctc tgaataagag gtaaagctcc
     3781 gactctatcg tttttacctt gccataataa caagcctttc agattcaaaa atatagggtt
     3841 atctggttga agttctaggc ttcgattaat gtgcgctaat gctaggtcaa tgttcgcagg
     3901 atttgcccca cttaactttg agtttgttcc atatacttgt aaattggctg ataactcatt
     3961 aaatatcgct tgtgcctcaa tatggctaag atcagcttca agttgcgtct tactactact
     4021 ttcaatgtct tgaagtgtat cttcaatttt aggaagtaaa gcctgtgctt cttcctttag
     4081 taattcactt aaatttttgg cttcttcctc atttagctga gtacaaaatt caattaggag
     4141 atctgaagca tctaaacttg cttgtctttc attaagattt ttttgctgta ttaaatcttc
     4201 tatgaaaact tgcctagctc ttactaaatc tcttacttca tttcttttca aaggtaagtg
     4261 aatatctact ccaaaaagat ttcctactaa ctttttatga tccatttcct acctctttcc
     4321 tttcgtcaca gcttaaagtt ttcctagttt tttctttcta taatatgtgc tttgtgaaat
     4381 acctagctct aaccaaggtt gagtagttct ttcagaattt tctaacttag gtctttttga
     4441 aacttttcca ccaagttgcc cttttctact ttgtctatca ataaactctt gataacaata
     4501 tgcgtctttc ttccagcaat acttggcaat actacgtgct gtagctttta tttctgaata
     4561 tggtaatggc tctagaaatg cactatttac gcttaaacac tcttttaata cggcacctag
     4621 ccaactatca aaagtacttc ctcggtattc tctaatcgct ttataggccc atttacgagc
     4681 aatatcgaac aaagtgcagt tcctacctag acctgttccc tgttctttct gtgtgagggg
     4741 ctttaaatcg acaaaatcgg caagataatt aagttcgtaa gcttctcttg tccaaatcgt
     4801 agtacgccag tacgtatgac agggattttt tgtcaataaa ccgctataac ctgaatccgc
     4861 acccatttgt cgtgccattc cggcttcaat aacagctaaa taatcaataa ttttctgttt
     4921 tgatgctgtt gttgtagaaa caggagcttt taattcatat ccaatatgag catgtccatt
     4981 cttaggattc tgacttgtcc acgttggttt tggtaaacct gcatcaaacc aagccatgac
     5041 cccatcagga cgatctaaat caaagacaag gtaaactatt ttgcaaggcg gattatgctg
     5101 caaatagcgt tttttaatcg caatctgttt tggattaatg aaggaatagc ctaaatcatc
     5161 tgtgcaatat ggcttatgcg gaattttttt ataaaaatct gctaaaaact gatcatctcg
     5221 cat
//
