LOCUS       NZ_CP032110             5324 bp    DNA     circular CON 31-MAR-2024
DEFINITION  Acinetobacter lwoffii strain EK30A plasmid pALWEK1.8, complete
            sequence.
ACCESSION   NZ_CP032110
VERSION     NZ_CP032110.2
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN05251105
            Assembly: GCF_019787625.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter lwoffii
  ORGANISM  Acinetobacter lwoffii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter.
REFERENCE   1  (bases 1 to 5324)
  AUTHORS   Rakitin,A.L., Ermakova,A.Y., Beletsky,A.V., Petrova,M.,
            Mardanov,A.V. and Ravin,N.V.
  TITLE     Genome analysis of Acinetobacter lwoffii strains isolated from
            permafrost soils aged from 15 thousand to 1.8 million years revealed
            their close relationships with present-day clinical isolates
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5324)
  AUTHORS   Rakitin,A.L., Ermakova,A.Y., Beletsky,A.V., Petrova,M.,
            Mardanov,A.V. and Ravin,N.V.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2021) Institute of Bioengineering, Research Center
            of Biotechnology of the Russian Academy of Sciences, Moscow 119071,
            Russia
  REMARK    Nucleotide sequence updated by submitter
REFERENCE   3  (bases 1 to 5324)
  AUTHORS   Beletsky,A., Kurakov,A., Petrova,M. and Mardanov,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2018) Lab of molecular cloning, Institute of
            Bioengineering, Research Center of Biotechnology of RAS, Prosp.
            60-let Oktyabrya, bld 7-1, Moscow 117312, Russia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Newbler v. 3.0
            Expected Final Version            :: yes
            Genome Coverage                   :: 25.0x
            Sequencing Technology             :: 454
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_019787625.1-RS_2024_03_31
            Annotation Date                   :: 03/31/2024 06:12:30
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,448
            CDSs (total)                      :: 3,336
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 112
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 222
            CDSs (without protein)            :: 222
            Pseudo Genes (ambiguous residues) :: 0 of 222
            Pseudo Genes (frameshifted)       :: 80 of 222
            Pseudo Genes (incomplete)         :: 138 of 222
            Pseudo Genes (internal stop)      :: 46 of 222
            Pseudo Genes (multiple problems)  :: 39 of 222
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP032110.2.
            On Aug 29, 2021 this sequence version replaced NZ_CP032110.1.
            Bacteria and source DNA available from Dr. Petrova M.A.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5324
                     /organism="Acinetobacter lwoffii"
                     /mol_type="genomic DNA"
                     /strain="EK30A"
                     /isolation_source="permafrost, Kolyma lowland"
                     /db_xref="taxon:28090"
                     /plasmid="pALWEK1.8"
                     /geo_loc_name="Russia"
     gene            24..311
                     /locus_tag="ABEKA_RS01425"
                     /old_locus_tag="ABEKA_3315"
     CDS             24..311
                     /locus_tag="ABEKA_RS01425"
                     /old_locus_tag="ABEKA_3315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004647572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="WP_159373217.1"
                     /translation="MTRSAENIEKKIEAQLEKLKQLKAQKQAIEARERSKQKEQERKDD
                     TRRKILLGSYLIKKMQSNEANKEKILMELNEYLTENRDRQLFDLPNIEEN"
                     /name="mobilization protein"
     gene            314..625
                     /locus_tag="ABEKA_RS01430"
                     /old_locus_tag="ABEKA_3316"
     CDS             314..625
                     /locus_tag="ABEKA_RS01430"
                     /old_locus_tag="ABEKA_3316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004647573.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="WP_159373219.1"
                     /translation="MNKADLIAKLAPKLNINQDEARVVLNSVLEVITEALVEEKNVRLV
                     DFGTFSVKQRPERMGHNPKTGEKLKIEAKEIVTFKQGKALGEQFIVKPKQKSKAKKKA"
                     /name="HU family DNA-binding protein"
     gene            complement(622..918)
                     /locus_tag="ABEKA_RS01435"
                     /old_locus_tag="ABEKA_3317"
     CDS             complement(622..918)
                     /locus_tag="ABEKA_RS01435"
                     /old_locus_tag="ABEKA_3317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004779687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_159373220.1"
                     /translation="MMKLTNLLEELHGTQAEYLDIVNYEIARENICGYIFLLSRISQNA
                     EPTEKMQMEGKIEDLIYYRDNLQIEDKENIQKVLAELIPEYKAEQEKQRGKKN"
                     /name="hypothetical protein"
     gene            complement(915..1844)
                     /gene="repM"
                     /locus_tag="ABEKA_RS01440"
                     /old_locus_tag="ABEKA_3318"
     CDS             complement(915..1844)
                     /gene="repM"
                     /locus_tag="ABEKA_RS01440"
                     /old_locus_tag="ABEKA_3318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004779686.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepM"
                     /protein_id="WP_159373226.1"
                     /translation="MKNGLVVKDNALINASYNLELTEQRLIMLAIINARELGQGITADS
                     KLEIHASDYAKLFNVSVDASYKALKEAVNNLFNRQFSYTAEYKKTGKVGVVRSRWVSRI
                     FYVDDLALLEITFAPDVVPLITRLEEHFTKYEAKQVAHLTSKYATRLYELLIAWREVGK
                     TPIFELQQLRKNLGVEDGEYQRMHHFKNRVLDTAITQINEHTDIKTTYEQHKQGRTITG
                     FSFRFKQKTQPKIEIKRDPNTPDFFIKMTDAQRHLFANKMSEMPEMSKYSQGTESFQQF
                     AIRIADMLLEPEKFRELYPCLEKAGFQA"
                     /name="repM"
     gene            complement(2571..3227)
                     /locus_tag="ABEKA_RS01445"
                     /old_locus_tag="ABEKA_3319"
     CDS             complement(2571..3227)
                     /locus_tag="ABEKA_RS01445"
                     /old_locus_tag="ABEKA_3319"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_159373212.1"
                     /translation="MFKYTESFVADLLFKENRIRIGTLNGFRDMESKQGISDPLEGSYD
                     DHVIINGTEKDYFNNPIFRENLKGLIHIEDSCSNITITNCTSLTKRVSQNFLIFCCADK
                     ESSDLFKQFDRADTCFTISKPHRFFTLITWALEKEFNCAVDFLGVHKISYQPYTRKRSA
                     FDSLNIHPALAKTEEFSPQCELRAIWQVPQDLITKPFYDLQVSGLKKTCSLINFE"
                     /name="hypothetical protein"
     gene            complement(3708..4178)
                     /locus_tag="ABEKA_RS01450"
                     /old_locus_tag="ABEKA_3321"
     CDS             complement(3708..4178)
                     /locus_tag="ABEKA_RS01450"
                     /old_locus_tag="ABEKA_3321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004811700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_159373213.1"
                     /translation="MSNQNDLDDQLYILLASMKEYREAIADDNKRLETFYNKVASGILE
                     QSKKTLNNANQEATRALQGRIQELDKATDKLNYRFIALLCAIFLSLVLVFLSFIFLFIP
                     SLDEIQQRRAEAAWLEKSYNLDIKNCNGKACVRIMKNDCHGTNKDYCVIDPK"
                     /name="hypothetical protein"
     gene            complement(4175..5131)
                     /gene="mobQ"
                     /locus_tag="ABEKA_RS01455"
                     /old_locus_tag="ABEKA_3322"
     CDS             complement(4175..5131)
                     /gene="mobQ"
                     /locus_tag="ABEKA_RS01455"
                     /old_locus_tag="ABEKA_3322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017284732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_159373215.1"
                     /translation="MAIYHCTTKTVNRSSGRTAVASMAYRAGEKLTDERTGLTHDFTRK
                     EGVVYTEILSNLDTELDRSKVWNLAEKSENRKDARTAREWVIALPDELNEEQRKELARE
                     FAQSLVDRYGVVADLAIHAPSKGGDDKNHHAHILLTTRKAELDTENKLVLTQKSEIELS
                     NTKRKSLGMGTSQEEIKQIRATWANLANHALEYAGYRERIDHRSYADQGNQLQATIHEG
                     SKVTQMRRKGIDTEISRFNDTIKQQNSQQLQYKQQHKEQTLEQGFNCVEKGFEQWKKDQ
                     EAKRLELEHKKQLLKLQQEQAMKLKQHKSMNRNGPSL"
                     /name="mobQ"
     Typing          complement(916..1844)
                     /name="rep_cluster_1265"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.462"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(4179..5131)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 cctatatccc gaaatgaacc gttatgacca gatcagccga aaatattgaa aagaaaattg
       61 aagctcagtt agagaagtta aaacagttaa aagcacaaaa acaagctatc gaagctagag
      121 aaagatcgaa gcaaaaagag caagaaagaa aggatgatac tcgacgtaaa attttgctcg
      181 gttcttattt aattaaaaag atgcaaagta atgaagccaa caaagaaaaa atactcatgg
      241 aacttaatga atatttaact gaaaatagag atagacagct ttttgactta ccaaatattg
      301 aggaaaatta attatgaata aagccgattt aattgccaaa ttagcaccta aattaaatat
      361 taatcaagac gaagcaagag ttgttcttaa ttctgttctt gaagtcatta cagaagccct
      421 agttgaagaa aaaaatgtcc gattagttga ttttggtact ttttcagtaa aacaacgccc
      481 agaaagaatg ggacacaatc ctaaaactgg agaaaaactt aaaattgaag ctaaggaaat
      541 cgttactttt aaacaaggta aggctttggg agagcaattt attgttaagc caaagcaaaa
      601 aagtaaagct aagaagaaag cctaattttt cttacctctt tgtttttctt gttcggcttt
      661 atactctgga attagctcag ctaagacctt ttggatattt tccttatctt cgatctgcaa
      721 attatcccga tagtaaatta agtcctctat tttgccttcc atctgcattt tttcggtggg
      781 ttcggcattt tgggatattc gggataatag gaagatataa ccgcatatat tttccctcgc
      841 aatttcataa tttacgatat cgaggtattc agcttgtgtg ccgtgaagtt cttctaatag
      901 gttggttaat ttcatcatgc ttgaaaccct gctttttcta aacatggata aagctctctg
      961 aacttttcag gctctaaaag catgtcagcg atacgaatag caaattgctg aaaactttcc
     1021 gtaccttgtg agtatttgct catttcgggc atttcagaca ttttattggc aaataagtgg
     1081 cgctgagcat cggtcatttt gatgaaaaaa tcaggggtat tgggatcacg tttaatttca
     1141 attttcggct gtgttttttg cttaaacctg aatgaaaacc cagtgatggt acgcccttgc
     1201 ttatgctgtt cataagtggt ttttatatcc gtatgctcat tgatctgggt aatagctgtg
     1261 tctaatacac ggtttttaaa atgatgcata cgttgatatt caccatcttc aacacctaaa
     1321 tttttacgta attgttgcag ttcaaaaatt ggtgttttac caacctcacg ccaggctatt
     1381 aataattcat aaagtctggt ggcgtactta ctggttaagt gtgcaacttg tttggcttcg
     1441 tatttcgtaa aatgttcttc taatcgagtg attaacggca ctacgtcagg tgcgaaagta
     1501 atttctaata gcgctaagtc atctacataa aaaatacgac tgacccaacg agagcgaaca
     1561 acacccactt ttcctgtttt tttgtattct gctgtatagc taaactgtct attaaataga
     1621 ttgtttacag cttcttttag agctttatag gatgcatcta ccgatacatt aaagagcttt
     1681 gcataatcac tagcatgtat ttctagtttg ctatctgccg tgatgccctg tcctaattcc
     1741 cttgcattaa ttatcgccag cataattaaa cgctgttctg ttagttctaa attataactg
     1801 gcgtttatta atgcattatc tttcacaact aaaccattct tcatattaaa tacacgttat
     1861 tattaatgtg tatttaaaca ctacatataa tacgtgtgta aatcaagaga atttgtgtgc
     1921 ttatacaaga gaatttgtgt gcttatacaa gagaatttgt gtgcttatac aagagaattt
     1981 gtgtgcttat tgagagtgaa accctctgat accaatgctt tcatagggtc taaaagcatt
     2041 taaaagcttt aaaaataatt aaaagccttt cggcagggga gtggaactgc cctaggtact
     2101 cgctaaagct cgtactctat tgaagctcgt tcctcgcttc gaggggcagt ttttaattag
     2161 tactgtttgt gatattcaaa agtaaaagca ttgataatgt attcgcttcg ctcatatttt
     2221 ttgaaaagca aaagctccaa agttcgcacc cattcgggat gctctatacc taccgtttaa
     2281 aatcgaaagg gagcttgtaa tcagagaaaa ggcgagcgtg aaattttgat gatgctcaat
     2341 cgctcgtaga cactcgctcg gttcttatta tctaggtttt aattatttaa tggagctaat
     2401 caaactcgtg aaacattgct taaataatag gcgtggaacg tgaaaaatca taaaaaaaag
     2461 cccaccttgg gggaggttgg gcaaaaaaga aactacagtg ttgaattata aggataatta
     2521 taacgcattt cgtataaggt gtattatgtt aattttagaa ttacttaaat ttattcaaaa
     2581 tttattaagc tacacgtttt tttcaaacca cttacttgta aatcataaaa aggcttcgta
     2641 attaagtcct gaggaacttg ccaaatagct cttaattcac attgagggct gaattcttca
     2701 gtttttgcca aagcaggatg aatatttaat gagtcaaatg cagatctctt acgtgtataa
     2761 ggctgataac taattttatg tacacctaaa aagtctactg cacaattaaa ttctttttct
     2821 aaggcccatg taattaaagt aaaaaaccta tgaggtttag atatagtaaa gcaagtatca
     2881 gcacgatcaa attgtttaaa tagatcacta gattccttat ctgcacaaca gaaaataagg
     2941 aagttttgag aaactctttt tgtaagacta gtgcagttag taatggttat attgctacag
     3001 ctatcttcta tatgtattaa accttttaaa ttttctctaa atattgggtt attaaaataa
     3061 tctttttcag ttccattaat tattacatga tcatcataag aaccttccaa tgggtctgat
     3121 ataccttgtt ttgattccat atctctgaat ccatttagag tacctatacg gatacgattt
     3181 tccttaaaca aaagatctgc tacaaaactt tctgtatatt taaacataga tataagcata
     3241 taatttaatt gatttttatt ttatcagatg tcaaaaatat ataattaaaa aatttcctct
     3301 atttaacata aaaactctta tacgaaattt aattattttt gctaaaacac tatttgaaaa
     3361 tataaaaaac cataaaaaaa ttatggtttt ttttgtatca acttgtttgt tgtctctatt
     3421 tcattgaata cttcaaatat tttccgatca ttattggatt taacaactag agttaaaata
     3481 aaaaatatta taaaagccag gaaaatcaaa tacattctca ttattaagtc cactattagt
     3541 gctatgaaag tatgcttcct tatgacaaat agataggaca taaaaaatga aaagtaaagc
     3601 tcgacacttt gaaatatggc ttttgccatg tttcaaagtc gagccacttt gcgacatccg
     3661 gtattgataa gattaagaga agaaccgagc aaagtactta ccagtaatta tttaggatca
     3721 atcacacagt aatctttatt cgttccgtga cagtcatttt tcatgatccg tacacaagct
     3781 ttaccattac aatttttaat atctaggttg taactctttt ctaaccaagc tgcttctgct
     3841 cgacgctgct ggatttcatc caaacttgga ataaataaaa atataaaaga caggaaaact
     3901 aataccagac taaggaaaat tgcacacaac aaggcaataa atctgtagtt caatttatcg
     3961 gtggctttat ctaactcctg aatacgacct tgaagggcac gtgtagcttc ctgatttgcg
     4021 ttatttaggg tctttttgct ctgttctagt atcccgctag ctaccttgtt gtaaaacgtc
     4081 tctaatcgct tgttatcatc tgctatggct tccctgtatt ccttcatgga tgccaataaa
     4141 atatataact ggtcgtctaa atcgttttga ttgctcataa ggaaggccca tttctattca
     4201 tgcttttgtg ttgtttcagc ttcatcgctt gttcttgctg tagctttaac aactgctttt
     4261 tatgttccag ttctaggcgt ttagcttcct gatctttctt ccattgctca aagccttttt
     4321 caacacagtt gaaaccctgt tctaaggtct gttccttgtg ctgttgtttg tattggagct
     4381 gttgactgtt ctgctgtttg atcgtgtcgt tgaaacggct aatttcagta tctatgccct
     4441 ttctgcgcat ctgggtgact ttactgcctt catgtatggt ggcttgtagc tgattacctt
     4501 gatcggcata actgcggtgg tcgattcttt cccgataccc tgcatattcc agagcatgat
     4561 tggctaagtt tgcccaggtg gctcgaatct gtttaatttc ttcctggctt gttcccatgc
     4621 ctaagctttt tcttttggtg ttacttaact caatttccga tttttgagtg aggaccagtt
     4681 tattttcagt atctaattct gctttgcgag ttgtcagtaa gatatgtgca tgatggtttt
     4741 tatcatctcc acctttgcta ggtgcatgaa tcgctaaatc tgcaaccacc ccataacggt
     4801 ctactaaaga ttgagcaaac tctcttgcta gttcttttcg ctgttcttca ttcagctcgt
     4861 ccggtagagc aatcacccat tcacgtgcgg ttcgggcatc tttccgattt tctgattttt
     4921 ccgccagatt ccaaactttg cttcggtcta attcagtatc caagttagaa agaatctctg
     4981 tatacaccac accttctttt ctcgtgaagt cgtgcgttaa ccctgtccgt tcatctgtca
     5041 atttttctcc tgcacgatag gccatagatg ctaccgcagt tcgtcccgaa cttcggttaa
     5101 cggtcttagt cgtacagtga taaattgcca tgccttagcc ttgtgatggt tttgcttttg
     5161 cttttaaaaa attgcgtagc aattttttgg ggttaagggg atatccctta cgcaaacttt
     5221 gacttggatg aaatccaagt cgtaagtgcg catttcatga aaaacacgat agcatatagc
     5281 ccgcttgaat tccaatttat agggccttat gcttgagtgg tttt
//
