LOCUS       NZ_CP032107             8635 bp    DNA     circular CON 31-MAR-2024
DEFINITION  Acinetobacter lwoffii strain EK30A plasmid pALWEK1.4, complete
            sequence.
ACCESSION   NZ_CP032107
VERSION     NZ_CP032107.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN05251105
            Assembly: GCF_019787625.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter lwoffii
  ORGANISM  Acinetobacter lwoffii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter.
REFERENCE   1  (bases 1 to 8635)
  AUTHORS   Mindlin,S., Petrenko,A., Kurakov,A., Beletsky,A., Mardanov,A. and
            Petrova,M.
  TITLE     Resistance of ancient and modern Acinetobacter lwoffii strains to
            heavy metals and arsenic revealed by genome analysis
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8635)
  AUTHORS   Beletsky,A., Kurakov,A., Petrova,M. and Mardanov,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2018) Lab of molecular cloning, Institute of
            Bioengineering, Research Center of Biotechnology of RAS, Prosp.
            60-let Oktyabrya, bld 7-1, Moscow 117312, Russia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Newbler v. 3.0
            Expected Final Version            :: yes
            Genome Coverage                   :: 25.0x
            Sequencing Technology             :: 454
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_019787625.1-RS_2024_03_31
            Annotation Date                   :: 03/31/2024 06:12:30
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,448
            CDSs (total)                      :: 3,336
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 112
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 222
            CDSs (without protein)            :: 222
            Pseudo Genes (ambiguous residues) :: 0 of 222
            Pseudo Genes (frameshifted)       :: 80 of 222
            Pseudo Genes (incomplete)         :: 138 of 222
            Pseudo Genes (internal stop)      :: 46 of 222
            Pseudo Genes (multiple problems)  :: 39 of 222
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP032107.1.
            Bacteria and source DNA available from Dr. Petrova M.A.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8635
                     /organism="Acinetobacter lwoffii"
                     /mol_type="genomic DNA"
                     /strain="EK30A"
                     /isolation_source="permafrost, Kolyma lowland"
                     /db_xref="taxon:28090"
                     /plasmid="pALWEK1.4"
                     /geo_loc_name="Russia"
     gene            425..622
                     /locus_tag="ABEKA_RS01295"
                     /old_locus_tag="ABWEK_D0002"
     CDS             425..622
                     /locus_tag="ABEKA_RS01295"
                     /old_locus_tag="ABWEK_D0002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005265040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_005265040.1"
                     /translation="MFNFKIFNRISTEVLTIKNDLQLNSEIQLITKYKTSTSEDYKKAI
                     ILIFKERGYSALEIGQLLEN"
                     /name="hypothetical protein"
     gene            complement(689..2566)
                     /locus_tag="ABEKA_RS01300"
                     /old_locus_tag="ABWEK_D0003"
     CDS             complement(689..2566)
                     /locus_tag="ABEKA_RS01300"
                     /old_locus_tag="ABWEK_D0003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003653920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="WP_005265041.1"
                     /translation="MQNNVSKPAVPALTLAALGVVFGDIGTSPLYALKESFHATRGLPI
                     NELNILGILSLIFWTITLIVSLKYVLVIMRADNNGEGGIMALLALNLRQKGLSRGKRLA
                     IIILGFIGASLFFGDGIITPAISVLSAVEGLSVAAPSFDRFIIPISIGILIALFAVQKH
                     GTAMMGKFFGPITLLWFLSIGAIGIISIIQSPFVLALFSPHWAFLFVINNPFISFFVMG
                     AVVLTVTGGEALYADMGHFGILPIKLGWFLIVLPCLMLNYAGQGALLLRDPSAITNPFY
                     LLLPSAFLYPMIILATAAAVIASQALISGVFSMAKQAIQLGYLPRLTVLHTSASEIGQI
                     YIPLLNWLLFISITILILIFESSSKLAGAYGLAVTVTMFCDTLLVAFLAYSYWKWRTWK
                     VLLFIIPFAFIDLILLSSNLLKVLIGGWVPVVIAVIVFTLMMTWKKGREILQDKLQKDT
                     LPLNVFLEHLEQTGQKVSGNAVFLTGTPQVVPHALLHNLKHNKVLHERNFLVTIKTSEI
                     PYVDEAKRIVTEVLENGFFRITIHYGFKEEPNVPHSLKQAFSVLDLEYDLMNISFFVSR
                     ERLIPSMSNKMSTWREKLFVAMQKNTSPVSDFYKIPSNRVVELGSQIEI"
                     /name="potassium transporter Kup"
     gene            3498..4427
                     /gene="repM"
                     /locus_tag="ABEKA_RS01305"
                     /old_locus_tag="ABWEK_D0006"
     CDS             3498..4427
                     /gene="repM"
                     /locus_tag="ABEKA_RS01305"
                     /old_locus_tag="ABWEK_D0006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871874.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepM"
                     /protein_id="WP_004871874.1"
                     /translation="MKNSLVVKDNALINASYNLELTEQRLIMLAIINARESGQGITADS
                     KLEIHASDYAKLFNVSADASYKALREAVNNLFNRQFSYTAEYKKTGKVGIVRSRWVSRI
                     FYVDDLALLEITFAPDVVPLITRLEEHFTKYEAKQVAHLTSKYATRLYELLIAWREVGK
                     VPQLELSEFRNRLGLVDSEYTAMSDFKKRVLEPSIKQINEHTDITVTYEQHKKGRIISG
                     FSFKFKQKQQPKVEAKRDPNTPDFFIKMTDAQRHLFANKMSEMPEMGKYSQGTESYQQF
                     AIRIADMLLEPEKFRELYPILEKSGFQP"
                     /name="repM"
     gene            4424..4720
                     /locus_tag="ABEKA_RS01310"
                     /old_locus_tag="ABWEK_D0007"
     CDS             4424..4720
                     /locus_tag="ABEKA_RS01310"
                     /old_locus_tag="ABWEK_D0007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004856442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_005265043.1"
                     /translation="MIKLNNLSTDLKHVTVEYLDIVNYEIARENICGYIFLLSRLSKNA
                     KPTEKMQMESKIQDLIYYRDHLQIEDKDNIQKVLNTLIPEYQAEQNNQTAKKN"
                     /name="hypothetical protein"
     gene            complement(4717..5001)
                     /locus_tag="ABEKA_RS01315"
                     /old_locus_tag="ABWEK_D0008"
     CDS             complement(4717..5001)
                     /locus_tag="ABEKA_RS01315"
                     /old_locus_tag="ABWEK_D0008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004871868.1"
                     /translation="MTKSAENIEKKIEAQLEKLKQLKAQKQAIEARERTKKKEQERKDD
                     TRRKILLGSYLIKKMQANEANKEKILAELNEYLTENRDRQLFDLPDIEA"
                     /name="hypothetical protein"
     gene            5188..6159
                     /gene="mobQ"
                     /locus_tag="ABEKA_RS01320"
                     /old_locus_tag="ABWEK_D0009"
     CDS             5188..6159
                     /gene="mobQ"
                     /locus_tag="ABEKA_RS01320"
                     /old_locus_tag="ABWEK_D0009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005265044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_005265044.1"
                     /translation="MAIYHCSTKTVNRSSGRTAVASSAYRAGEKLEDERTGLTHDFTRK
                     DGVAHSEILSNLDIEIDRGELWNLAEKTENRKDARTAREWVIALPDELDADQRKDLAKD
                     FARSLVDRYGVIADLAIHEPSKGGNDKNHHAHIMLTTRKAELDTDNKLTLTTKTDIELS
                     NAKRKSLGMGTTQEDIKQIRETWADLANKALERAGYREKIDHRSYADQNNGLQATIHEG
                     TKVTQLRRQGIDTEISRFNDNVKQQNSQQLHQQKQQKESVLQRGLSRVDQGFDQWQKNQ
                     ETKRLELERQAEMKRQQELDKQRAEQALRKASQKLGRGGMSL"
                     /name="mobQ"
     gene            6156..6653
                     /locus_tag="ABEKA_RS01325"
                     /old_locus_tag="ABWEK_D0010"
     CDS             6156..6653
                     /locus_tag="ABEKA_RS01325"
                     /old_locus_tag="ABWEK_D0010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004969149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004282248.1"
                     /translation="MSNQNDLDDQLYILLASMKEYREAIADDNKRLEQFYNHVASGVLD
                     KAEKSLENANKKHTGALNNSIQALNEATHRLNLKLIIIFASTFVAVMMVFILAIFLYIP
                     SKDEIDERRADMAVLKKYPLQIRESDGETLVRIMRKNCYSFEPSSVKEKTYDWCQIDPK
                     KY"
                     /name="hypothetical protein"
     gene            6909..8429
                     /locus_tag="ABEKA_RS01330"
                     /old_locus_tag="ABWEK_D0011"
     CDS             6909..8429
                     /locus_tag="ABEKA_RS01330"
                     /old_locus_tag="ABWEK_D0011"
                     /EC_number="1.11.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005249302.1"
                     /GO_function="GO:0004096 - catalase activity [Evidence
                     IEA]; GO:0020037 - heme binding [Evidence IEA]; GO:0046872
                     - metal ion binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="WP_005265045.1"
                     /translation="MSQDPKKCPVTHLTTEAGAPVVDNQNSMTAGARGPLLAQDLWLNE
                     KLANFVREVIPERRMHAKGSGAFGTFTVTNDITQYTRAKIFSEVGKKTEMFARFSTVAG
                     ERGAADAERDIRGFALKFYTEEGNWDLVGNNTPVFFLRDARKFPDLNKAVKRDPKTNKR
                     SATNNWDFWTLLPEALHQVTIVMSDRGIPAGYRHMHGFGSHTFSFINAQNERFWVKFHM
                     RTQQGIQNLTDAEAADLIAKDRESSQTDLFDAIERGDYPKWKMYVQVMPELEAETVPYH
                     PFDLTKVWPKGDYPLIEVGEFELNRNPENYFQDVEQAAFAPSNLVPGISYSPDRMLQAR
                     LVNYADAARYRVGVNHSQVPVNAARCPVNSNRRDGQGRMDGNYGSLPHYEPNSFNQWQE
                     QPQFKEPALKITGDADFWDFREDDNDYFSQPRALFNLMNDEQKQALFNNTAAAMGDALD
                     FIKYRHIRNCYACDPAYGQGVAKALGMTVTDAQAARETDPAKGLPSFL"
                     /name="catalase"
     Typing          3499..4431
                     /name="rep_cluster_1219"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="81.906"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          5189..6156
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.427"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gtgagccaaa gcgtacaaaa tagcctaaat ttcatccaca gaaattgggg atagttttca
       61 ccctggaaga tttgataaca ttgggaaaat gccaaaattg actatttttc aaccaatttt
      121 attggcaaga actatcaaaa acgctcataa aaaagccgac ttgtttccaa gtcagctttt
      181 cgacatcgtc gagcattatc actataacac aagtatttga tttttatgtg tttaaaatat
      241 gcatttcgta taacgcccat tatgttaaat ggatagtact ttagtggatt attcatgctt
      301 cccatttaga aaaacttgcg gagccttttc taatctgtaa tccttgccac taagggtgtc
      361 tagctaagct aaggcaggtg tttattgtag tcgggccgct acaatgctga taaagagcac
      421 ctgtatgttt aatttcaaga tttttaatag aatctctacg gaagtattga cgataaaaaa
      481 tgacctccag cttaattctg agattcaact cataacaaaa tataagacct ctacttctga
      541 agattataaa aaagcaatta ttttgatctt taaagaacgt ggttattcag ctttagagat
      601 tggtcaacta ttggaaaatt aaagtaaaag atttattctg atcagatagg cacccttatt
      661 taaaggtgcc tattcatttt agaaattctt aaatttcaat ctgactacct agctctacca
      721 cccggttaga agggatttta taaaaatcac tgacagggct ggtatttttt tgcattgcga
      781 caaataactt ttcacgccat gtagacatct tgttggacat acttggaatt aatctttctc
      841 tagaaacaaa gaagctgata ttcattaagt catactcaag atccagtact gaaaaagctt
      901 gtttcaatga atgaggcaca ttaggctctt ctttaaagcc ataatggatc gttattctga
      961 aaaaaccatt ctctagtact tcagtaacga ttcgcttagc ttcatcaaca tacgggattt
     1021 cagaagtctt gattgtcacc aaaaaattac gttcatgcag aactttatta tgctttagat
     1081 tatgtaaaag cgcatggggt acgacttgag gcgttccagt taaaaatacg gcatttccac
     1141 tcactttctg acctgtctgt tcaaggtgct caagaaacac attcagtgga agcgtgtcct
     1201 tctgcagttt gtcctgaaga atctctctgc cttttttcca ggtcatcata agggtaaaaa
     1261 cgataactgc aataacgact ggcacccagc caccaatcag gacttttaat aagtttgaac
     1321 ttagtaaaat tagatcaata aaggcgaacg gaataatgaa cagcaagact ttccaggttc
     1381 tccatttcca gtaactataa gccagaaaag ccactagcag ggtgtcacag aacatggtca
     1441 cagtgacggc cagaccgtag gcacctgcca atttactgct actttcaaat attaagatca
     1501 aaatagtgat ggagataaac aggagccagt tcagtaaagg aatataaatt tgtccaattt
     1561 ccgaagcaga ggtatgtaaa actgtaagcc taggtagata tcccaactgg attgcctgtt
     1621 tagccataga gaatacccca ctgattaaag cctgtgaggc aatcactgct gctgcagtag
     1681 ctagaataat cattggatag agaaaggctg aaggcagaag taggtaaaaa ggattagtaa
     1741 tagccgaagg atctcttaac agtaatgctc cttgcccagc ataattcagc atcagacatg
     1801 gcagtacaat taaaaaccag cctaacttga tcggtaaaat accaaaatgt cccatatccg
     1861 catataaggc ttcaccacct gttacggtta aaaccacagc ccccatcaca aaaaatgaaa
     1921 taaaaggatt gttaataacg aataaaaaag cccagtgcgg gctgaatagt gcaagaacaa
     1981 atggactttg aataatactt ataatgccaa tggcaccaat agatagaaac cataacaggg
     2041 taataggacc aaaaaatttc cccatcattg cagtaccatg tttttgaacg gcaaagaggg
     2101 ctatcagaat tccaatactg atgggaataa tgaagcgatc aaatgacggg gcagcaacag
     2161 aaaggccctc aactgcagat agaacagaaa tggcaggagt gataataccg tcaccgaaga
     2221 agagtgatgc tccaatgaaa cctaagataa tgattgcaag tctttttcca cgggataatc
     2281 ctttttgcct aaggtttaag gcgagtaatg ccatgatacc cccttcacca ttgttatcag
     2341 ctctcatgat caccaaaaca tatttaagcg aaacaattaa ggtaatggtc caaaagatta
     2401 aagagagaat tcctaggata ttaagttcat taataggtag cccgcgtgtc gcatgaaagc
     2461 tttcttttaa tgcatataat ggactcgttc ctatatcgcc gaatactact cctagggcag
     2521 ccagtgttaa tgctggtact gccggcttac tcacattatt ttgcatatat ttgatttaca
     2581 aaattgggga gcctcgaatt ttatcatctg gcttctcatg aaattgcctt gtacataaaa
     2641 acccatactt tcttagagtg ttatagtttt tctagctaag cctgcactat ataggtgcta
     2701 tttcacttat aagtaatcca aaaatatatg attctttaat agataatttt aagttttttc
     2761 taaaattaac ataatacgcg ttatacgaaa tcaatacatt ttatctttag gtttcaatga
     2821 tatattaaga ttttacaagc ctacattccc cagtgtaggc tttttttata atttttcatg
     2881 ttccacgctt actatttgat cattgttcca cggttttgat tagatatttt aattattaaa
     2941 cttcttccag taatagccga gcgagtgtct acgagcgacc acctggcatt aaaatttcgc
     3001 ccctgccttt tccctgatta caagctccct tttgcttcat aacgaaagat acagagcatc
     3061 ccgaatgggt gcgaactttg gagcttttgc ttttcaaaaa gtatgagcga agcgaataca
     3121 ttacaaatgc ttttgctttt agatttcaca acaggattaa ctaaaaactg ccccacgaag
     3181 cgaggaacga gcttcaatag agtacgagct ttagcgagta catagggcag ttttaagcac
     3241 tcccctctcg aaaggctttt aattattttt aaagctttta atgcttttag acaggttgaa
     3301 acccttgtgt agtaagtgtt acagactata taaacggaca tttgctcttg tataaacgga
     3361 catttgctct tgtataaacg gacatttgct cttgtataaa cggacatttg ctcttaatta
     3421 aaagacatta taaatattga ccttaaaaag atattagatt acattgtcct ttaattagat
     3481 aataataagt tcttcttatg aaaaatagtt tagtcgtgaa agataatgct ctaataaatg
     3541 ccagttacaa cctagagcta acggaacaac gtttgatcat gctagctatc attaatgcta
     3601 gagaatcggg acaaggaatc actgcggata gtaaactgga aatacatgct agtgactacg
     3661 ccaagctatt caatgtatct gccgatgcgt catataaagc tttgagagaa gctgtaaata
     3721 atctgtttaa tcgtcaattt agttatacag ccgaatataa aaaaacaggc aaagtcggca
     3781 ttgttcgatc tcgctgggta agccgtatat tttatgtaga tgacctagca ttgttagaaa
     3841 ttacttttgc tcctgatgta gtgcctttaa tcacacgatt ggaagaacac tttacaaaat
     3901 atgaagctaa acaagttgct cacctaacca gtaaatacgc cactaggctt tatgaattgc
     3961 tgattgcttg gcgtgaagtt ggaaaagttc cacaactaga acttagtgag tttagaaaca
     4021 gattaggtct tgttgatagt gaatacaccg ccatgagtga ctttaaaaag cgtgtgcttg
     4081 aaccatcaat taaacaaatt aatgagcata ctgatattac tgtaacgtat gaacagcata
     4141 aaaaaggacg gatcatttca ggtttttctt tcaaatttaa gcaaaaacaa cagccaaaag
     4201 ttgaagcaaa aagagatcca aacacgcctg atttttttat aaaaatgacc gatgcacaac
     4261 gccatttatt tgcgaataaa atgtcagaaa tgcctgaaat ggggaaatat tcacaaggta
     4321 cagaaagcta ccaacaattt gctattcgta tcgctgatat gcttttagag cctgaaaaat
     4381 tcagagagct ttatccaatt ttagaaaaat caggatttca gccatgatta aattaaataa
     4441 cctatcaacc gatctaaagc atgtgaccgt tgaatacctc gacatcgtaa attatgaaat
     4501 tgcgagggaa aatatatgcg gttatatctt cctattatcc cgactatcca aaaatgccaa
     4561 acccaccgaa aaaatgcaga tggaaagcaa aatacaggac ttaatttact atcgggatca
     4621 tttgcagata gaggacaaag acaatattca aaaggtttta aacacgctca ttcccgagta
     4681 tcaagccgaa cagaataacc aaacagctaa gaaaaattag gcttcaatgt cgggcaaatc
     4741 aaaaagctgt cgatctctat tttctgttaa atattcgttg agttcagcga gtattttttc
     4801 tttattggct tcatttgcct gcattttctt aatcaagtaa gaaccgagca aaattttacg
     4861 cctagtatca tccttgcgct cctgttcttt ctttttggta cgttctcttg cttcaatagc
     4921 ctgtttctgt gcttttaact gtttcagctt ctctaactgg gcttcaattt tcttttcgat
     4981 attttcagca gatttagtca taacggttaa tttcggaata tagaaaacca ctcaagcata
     5041 agaccctatg aattgaaatt caagcgggta atatgctatc gtgtttttca tgaaatgcgc
     5101 acttacgact tggatttcat ccaagtcaaa gtttgcgtaa ggggaatccc cttaacccca
     5161 aaagcaaaag caccccaagg cgactgcatg gcaatttatc attgttccac taaaacggtg
     5221 aaccgaagtt cggggcgaac tgcggttgca tcatctgcat atcgtgcagg ggaaaaacta
     5281 gaagatgaac gcacaggact gacccatgac tttacgagaa aagacggtgt cgcccattct
     5341 gaaattctgt ctaatttaga tattgaaatt gaccgtggtg aactttggaa cttggcagaa
     5401 aaaaccgaaa accgtaaaga tgcccgaacc gctagagaat gggtcattgc cctacctgat
     5461 gaattagacg ctgatcaacg taaggacttg gcaaaagact ttgccaggtc cttagttgat
     5521 cgctatggag tgatcgcaga tttagccatc catgaaccca gcaaaggcgg taatgataag
     5581 aatcatcacg cccatatcat gcttaccacc cgaaaagccg aattggacac ggacaataaa
     5641 ctcaccctga ccaccaaaac cgatatcgaa ctcagcaacg ccaaacgaaa aagccttggc
     5701 atggggacaa cccaagaaga tattaagcaa attcgagaaa cttgggcaga cttagccaat
     5761 aaagcattgg aacgtgcagg ctaccgagaa aaaatagacc atcgtagcta tgccgatcag
     5821 aacaacggac tacaagccac catccatgaa ggcaccaaag tcacacaatt acgcagacaa
     5881 ggcatagaca ctgaaatcag ccgttttaat gacaacgtca aacagcaaaa tagccaacag
     5941 cttcaccaac agaaacagca aaaagagagc gttttacagc gtggtttaag ccgtgtcgat
     6001 caaggttttg atcaatggca gaagaaccaa gaaaccaaac gtctagaact ggaacgccaa
     6061 gcggaaatga agcgacagca agaattagat aaacaacgag ccgagcaagc actccgcaag
     6121 gcatctcaaa aactaggtag aggcggaatg tcattatgag taatcaaaat gatctagatg
     6181 accagttata tattttattg gcatccatga aggaatatag agaggccata gcagacgata
     6241 acaagcgatt agagcagttt tacaaccatg tcgctagcgg agtgctagac aaggctgaaa
     6301 aatcgctaga aaacgcaaat aagaagcata caggcgcatt aaacaacagt attcaggcat
     6361 taaacgaagc tacacacagg ctcaacttaa aacttatcat tatttttgcc agcacgtttg
     6421 ttgctgtgat gatggtgttc attttggcta tctttttata cataccaagc aaagacgaaa
     6481 ttgatgaacg tagagcagat atggctgtac taaaaaaata tccgctacaa attcgagaaa
     6541 gtgatggtga aacattggtg agaataatga ggaaaaattg ctactcgttt gaaccgagta
     6601 gtgtcaaaga aaaaacgtat gactggtgtc agatcgaccc taaaaaatac tagttgatcg
     6661 gtactttgct tggtttttct cattacaaat caaattacag caagcgcaaa attgcccgac
     6721 tttgaaacat ggctttagcc atctttcaaa gcatcgagct gaaatttcgc ataagagatt
     6781 ttatgttaaa tcagttggct ataaattttc aaaaatatcc ttagtttaat attttaataa
     6841 gcaagtaaag ggtaagatag gattgaaaat cttaaatttc tgcattaaaa gaaacgggag
     6901 ttaatagtat gagtcaagac cctaaaaaat gtcctgttac ccacctgact actgaagctg
     6961 gtgcccctgt ggtggataat cagaacagta tgacggcagg tgcgcgtgga cctttacttg
     7021 cccaagattt atggttgaat gaaaaattag caaacttcgt ccgcgaagtg attccagagc
     7081 gtcgtatgca cgctaaaggt tcaggtgctt ttggtacctt taccgtgacc aatgatatta
     7141 cccaatatac tcgcgctaaa attttctctg aagtcggcaa aaaaaccgaa atgtttgcgc
     7201 gtttctcaac ggtggcgggt gaacgtggcg cggccgatgc agaacgtgat attcgaggtt
     7261 ttgccctgaa attctatact gaagaaggca actgggatct ggtcggcaat aataccccgg
     7321 tgtttttcct gcgggatgcc cgtaagttcc ctgacctgaa taaagcagtc aaacgtgacc
     7381 cgaaaaccaa caagcgcagt gccaccaata actgggattt ctggacatta ttgcctgaag
     7441 ccttgcatca ggtgaccatt gtcatgtcag atcgcggtat tcctgctggc taccgtcata
     7501 tgcatggttt tggcagccat accttcagtt ttatcaatgc ccaaaatgaa cgcttctggg
     7561 tgaaattcca tatgcgtacc cagcaaggca tccagaacct gaccgatgct gaagcagcag
     7621 acttgattgc caaagaccgt gaaagcagcc agaccgacct gtttgatgcc atcgaacgtg
     7681 gcgactatcc aaaatggaaa atgtacgttc aagtcatgcc agaactggaa gccgaaacag
     7741 tgccttatca tccatttgac ctgaccaaag tctggccgaa gggcgattat ccactgattg
     7801 aagtcggtga atttgagctg aaccgcaatc cggaaaacta tttccaggat gtagaacagg
     7861 cagcctttgc acctagtaat cttgtaccgg gcatcagcta ctcaccggac cgtatgttac
     7921 aggcacgttt agtgaactat gccgacgcag cgcgttaccg tgttggtgta aatcattcac
     7981 aggttccggt caatgctgca cgctgccctg taaactctaa tcgtcgtgat ggtcagggcc
     8041 gcatggatgg caactatggt tcattgccac attatgaacc gaacagtttt aaccagtggc
     8101 aggaacaacc tcagtttaaa gaaccagcat taaagattac cggtgatgct gatttctggg
     8161 acttccgtga agatgacaat gattacttca gccagccacg tgccctgttc aatttgatga
     8221 atgatgagca gaaacaggcc ctgtttaata acacggccgc agcgatgggt gatgcgctag
     8281 atttcatcaa ataccgtcat atccgcaact gttatgcttg cgatccagct tatgggcaag
     8341 gcgttgctaa ggccttaggt atgactgtaa cagatgctca agcggcacgt gaaacagatc
     8401 ctgctaaagg tttgccaagt tttctttaat gtggaaattt gatttagata gttaataaaa
     8461 aaaccccggc atcctgccgg ggtttttcgt attggttcgc tcggtataac tcctgtcgta
     8521 ccttacagtc cttgtgctta tctcttgtta ttcttgtatg gaacttcctg ttcccgatga
     8581 gttcatcata ggaaaattca cctatcctgg acagcagcaa agggccgagt ttcat
//
