LOCUS       NZ_CP032106            10347 bp    DNA     circular CON 31-MAR-2024
DEFINITION  Acinetobacter lwoffii strain EK30A plasmid pALWEK1.3, complete
            sequence.
ACCESSION   NZ_CP032106
VERSION     NZ_CP032106.2
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN05251105
            Assembly: GCF_019787625.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter lwoffii
  ORGANISM  Acinetobacter lwoffii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter.
REFERENCE   1  (bases 1 to 10347)
  AUTHORS   Rakitin,A.L., Ermakova,A.Y., Beletsky,A.V., Petrova,M.,
            Mardanov,A.V. and Ravin,N.V.
  TITLE     Genome analysis of Acinetobacter lwoffii strains isolated from
            permafrost soils aged from 15 thousand to 1.8 million years revealed
            their close relationships with present-day clinical isolates
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10347)
  AUTHORS   Rakitin,A.L., Ermakova,A.Y., Beletsky,A.V., Petrova,M.,
            Mardanov,A.V. and Ravin,N.V.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2021) Institute of Bioengineering, Research Center
            of Biotechnology of the Russian Academy of Sciences, Moscow 119071,
            Russia
  REMARK    Nucleotide sequence updated by submitter
REFERENCE   3  (bases 1 to 10347)
  AUTHORS   Beletsky,A., Kurakov,A., Petrova,M. and Mardanov,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2018) Lab of molecular cloning, Institute of
            Bioengineering, Research Center of Biotechnology of RAS, Prosp.
            60-let Oktyabrya, bld 7-1, Moscow 117312, Russia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Newbler v. 3.0
            Expected Final Version            :: yes
            Genome Coverage                   :: 25.0x
            Sequencing Technology             :: 454
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_019787625.1-RS_2024_03_31
            Annotation Date                   :: 03/31/2024 06:12:30
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,448
            CDSs (total)                      :: 3,336
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 112
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 222
            CDSs (without protein)            :: 222
            Pseudo Genes (ambiguous residues) :: 0 of 222
            Pseudo Genes (frameshifted)       :: 80 of 222
            Pseudo Genes (incomplete)         :: 138 of 222
            Pseudo Genes (internal stop)      :: 46 of 222
            Pseudo Genes (multiple problems)  :: 39 of 222
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP032106.2.
            On Aug 29, 2021 this sequence version replaced NZ_CP032106.1.
            Bacteria and source DNA available from Dr. Petrova M.A.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10347
                     /organism="Acinetobacter lwoffii"
                     /mol_type="genomic DNA"
                     /strain="EK30A"
                     /isolation_source="permafrost, Kolyma lowland"
                     /db_xref="taxon:28090"
                     /plasmid="pALWEK1.3"
                     /geo_loc_name="Russia"
     gene            1..924
                     /gene="repM"
                     /locus_tag="ABEKA_RS01245"
                     /old_locus_tag="ABEKA_3282"
     CDS             1..924
                     /gene="repM"
                     /locus_tag="ABEKA_RS01245"
                     /old_locus_tag="ABEKA_3282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004282204.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepM"
                     /protein_id="WP_004282204.1"
                     /translation="MKTELIVKDNALINASYNLDLVEQRLILLAIVEARESGKGINAND
                     PLTVHAESYINQFGVHRNTAYQALKDACDDLFARQFSYQSLSEKGNVINHKSRWVSEVA
                     YIDNEAVVRLIFAPAIVPLITRLEEQFTKYEIQQISNLTSAYAVRLYEILIAWRSTGKT
                     PLITIYDFRQKIGVLETEYKRMYDFKKYVLDIALKQVNEHTDINVKVEQHKTGRSITGF
                     SFSFKQKKSATNTANDISQDKELKINLTDAQRYLFASKLSELPEMAKLSQGNESYEQFA
                     ARIVTMLQEPNKLKEFMPLLRKVGFQ"
                     /name="repM"
     gene            992..1528
                     /locus_tag="ABEKA_RS01250"
                     /old_locus_tag="ABEKA_3283"
     CDS             992..1528
                     /locus_tag="ABEKA_RS01250"
                     /old_locus_tag="ABEKA_3283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004786457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication DNA-binding protein"
                     /protein_id="WP_004786457.1"
                     /translation="MKKLSVSELAKLYGYSRQAVYAHIKKGNLSKGSDGLIDFSEALRV
                     FGEPQKSNSSVNQSQSTSRHNLTEVDLLKRQVDMLEKQLNQAIQRENQSLERESFYQEQ
                     IEAMQRLLEAPKANMTTFTDHESEQDIATDSRPQTESNYDGLTTPENKRIPVPEHIEPT
                     PKKRGFLSRFFLPYG"
                     /name="plasmid replication DNA-binding protein"
     gene            complement(1811..3265)
                     /locus_tag="ABEKA_RS01255"
                     /old_locus_tag="ABEKA_3284"
     CDS             complement(1811..3265)
                     /locus_tag="ABEKA_RS01255"
                     /old_locus_tag="ABEKA_3284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_004995702.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="WP_159373207.1"
                     /translation="MLSNVREQWFSNIRRDVLAGLVVGLALIPEAIAFSIIAGVDPKVG
                     LYASFCIAVVISFVGGRPAMISAATGAMALLMVTLVKEHGLEYLLAATILTGVIQILAG
                     YLKLAKLMRFVSKSVVIGFVNALAILIFMAQLPELINVTWHVYALVALGLAIIYLFPLV
                     PQLGKLLPSPLVCIIAITLLAIALGIDVRTVGDMGSLPDTLPMFLIPNIPLNIETLMII
                     LPYSVALAAVGLLESMMTATIVDEMTDTPSDKYQECKGQGIANIASGFMGGIAGCAMIG
                     QSMINVKSGGLTRLSTFSAGIFLLILVVFISDWLKVIPMAALVAVMIMVSISTFEWSSV
                     TQLKDNPKSSNIVMIATVIVVVATHNLALGVLTGVLLSALFLANKLENDIQVTASFEGT
                     ARLYDLRGQIFFSSSEKFMQGFDFKEDVKDVIIDLTHSHIWDVTSVAMLDSVVNKFQKN
                     GVNVTVRGLNEASSIMIDKYGTHARI"
                     /name="SulP family inorganic anion transporter"
     gene            complement(3515..3724)
                     /locus_tag="ABEKA_RS01260"
                     /old_locus_tag="ABEKA_3285"
     CDS             complement(3515..3724)
                     /locus_tag="ABEKA_RS01260"
                     /old_locus_tag="ABEKA_3285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004282305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004282305.1"
                     /translation="MFNFKIFNKVSTEVLTIKNDLQLNAELQLINKYKTAISEDYKQAI
                     VLIFKERGYTRLEIGQLLGELKAS"
                     /name="hypothetical protein"
     gene            4497..5177
                     /locus_tag="ABEKA_RS01265"
                     /old_locus_tag="ABEKA_3287"
     CDS             4497..5177
                     /locus_tag="ABEKA_RS01265"
                     /old_locus_tag="ABEKA_3287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017816334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3761 domain-containing protein"
                     /protein_id="WP_222110376.1"
                     /translation="MIDINKKSLKKIISICTLTIFNIGCVSLEPQSIDSKTQDKKITSE
                     TIFIEEKDSINQLLQKVPEPLEVKIVGYVNKDLLQMFSSSTRSVVVNNVTRGTRVLIFD
                     TSGNLANISADIEYPYWVDLAGICFTENCWKQENTPTVKSSVNTRRTSPSLITPPKSST
                     QNKGISSNQGKGYTNVDGKYVPSPRKANKQPSNATAKCRDGTWSFSLNRRGTCSGHGGV
                     ANWL"
                     /name="DUF3761 domain-containing protein"
     gene            complement(5316..5981)
                     /locus_tag="ABEKA_RS01270"
                     /old_locus_tag="ABEKA_3288"
     CDS             complement(5316..5981)
                     /locus_tag="ABEKA_RS01270"
                     /old_locus_tag="ABEKA_3288"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_159373201.1"
                     /translation="MKFGLICEGVTDFHVLKHVIQSFFNGEAEFQPIQPFLDETHKKTA
                     EGNFGGWELVASYLKSQDLEDAVVNTDYLVIQIDTDVCEHPNFDVSPISLADSDHDQFY
                     ERIKLKLIEWIDSFEPDTYEYYKEKIIFAISVHCLECWLLAYHGQKKCKITGCEPELTA
                     TLRRKSKVLNTVNKNVREYIEHSQDLKKRKNHTAILKNSKSFVLFVDQLEKIPRDNPD"
                     /name="hypothetical protein"
     gene            complement(5994..7283)
                     /locus_tag="ABEKA_RS01275"
                     /old_locus_tag="ABEKA_3289"
     CDS             complement(5994..7283)
                     /locus_tag="ABEKA_RS01275"
                     /old_locus_tag="ABEKA_3289"
                     /inference="COORDINATES: protein motif:HMM:NF024573.4"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA];
                     GO:0016887 - ATP hydrolysis activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="WP_159373203.1"
                     /translation="MLAGIKIHNYKSIKELEVGVGQFNVLIGENGAGKSNFLEVLASGS
                     AIIANKFSNEFMLSRGIRIVNPDSLFSCFNNNMADNINITNVYENGYGCVFNIKFNKDE
                     PFAQLESDLLILHRNLDLNDDLEIVDIKDFNSNFLKDKIKDLAETTYGSVESLLKNLNE
                     IRDSAEKKEKDSKNDIKMVSNMVDYINTLGAIQRTVFDKYYSKNETSKESNFVIFSPEL
                     SSLRNFSSESQIEPLGINGEGLFKLLQVMQQHEQESFLQVCETLEMFQWVEKIIVDDQS
                     SSEQKVRIIDRFMGREIDHRSANEGFLFVLFYAALFSSKYTPDFFAIDNIDASLNPKLC
                     RVLIKKLVELAKANKKQVFVTTHNPAILDGLDLKDDEQRLFKVERDDDGATQLKRINIE
                     DLPKPKRNGETLRLSEAFMRGLLGGLPTNF"
                     /name="ATP-binding protein"
     gene            complement(7673..7864)
                     /locus_tag="ABEKA_RS01280"
                     /old_locus_tag="ABEKA_3290"
     CDS             complement(7673..7864)
                     /locus_tag="ABEKA_RS01280"
                     /old_locus_tag="ABEKA_3290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004282089.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="WP_004728112.1"
                     /translation="MRLLIALIFPWLLFFTIGRPFAGIICLLLQITVLGWIPAAIWAVY
                     ALSQYKTDQKIKRTIRSS"
                     /name="YqaE/Pmp3 family membrane protein"
     gene            complement(7894..8175)
                     /locus_tag="ABEKA_RS01285"
                     /old_locus_tag="ABEKA_3291"
     CDS             complement(7894..8175)
                     /locus_tag="ABEKA_RS01285"
                     /old_locus_tag="ABEKA_3291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005098300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="WP_159373209.1"
                     /translation="MSITETLDSKIKAQEEKLKQLKAQRQAALARERAKEKQQTRKDDT
                     RRKILIGSCMLKITEEDEQARAKLIAQMDKYLMDERDRKLFDLPAFNY"
                     /name="mobilization protein"
     gene            8385..9821
                     /gene="mobQ"
                     /locus_tag="ABEKA_RS01290"
                     /old_locus_tag="ABEKA_3292"
     CDS             8385..9821
                     /gene="mobQ"
                     /locus_tag="ABEKA_RS01290"
                     /old_locus_tag="ABEKA_3292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005248576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_159373205.1"
                     /translation="MAIYHFSVKTVARSAGRSATAAIAYRAGEKIYCEREGREHDYSRK
                     TGVEYKEIYLPEDAPKHLKNREKLWNEVEQRETRKNSTVAREFEIAFPSELNQEQRLAM
                     LEELCASIVDRHQVAVDACIHAPHTGSGSDERNYHAHILMSTRKLTPEGFTEKTRELDQ
                     KHSGEIEHWREHFADICNMHLDLAGYTARVDHRSYKDQENGLEATLHEGPKVTELRRKG
                     IETEISRSNDEIKQRNQAQLQYGKNMDLLIAENEIKLSTLKTEQQIQIKNSAKTPPIDE
                     KALFEEKQRETLGKVLKREISAKDANLDLDFMQQHLEKAEKTLSKHNKHQNEFNQQLAQ
                     EIVKNGLKQSHDKLQSLVDQHNELTQNKPLLFGKKAWEAQRDEIYQEHKKLKGQHEHQK
                     KHGVKDLLDNEKFKEHAWKQYQQQHPAKAKQYQTLYPSYQVIKKCVNEIKAEQQMKLRQ
                     EQQLKAQQHAPKLKSRGMSR"
                     /name="mobQ"
     Typing          2..924
                     /name="rep_cluster_1254"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.567"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          8386..9818
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="89.749"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atgaaaaccg aactaattgt caaagataac gctttaatta atgctagtta taaccttgat
       61 ctagtagaac aacgattaat tcttttagct atcgttgaag ccagggaatc aggtaaaggg
      121 ataaatgcta atgatccatt aacagttcat gctgaaagtt atatcaatca atttggtgta
      181 catcgaaata ctgcttatca agccttaaaa gatgcttgcg atgacctatt tgcaagacaa
      241 ttcagttatc aaagtcttag tgaaaaaggt aatgtcatta atcacaagtc aagatgggta
      301 agcgaggttg cttatattga taatgaggct gtcgttagac ttatttttgc tccagctatt
      361 gtgcctttaa taactcgatt agaagaacaa tttacaaagt atgagataca gcaaataagc
      421 aatttaacga gtgcttatgc tgttcgctta tatgaaatat tgattgcgtg gcgtagtacc
      481 ggaaaaacgc ctcttataac tatctacgat ttcagacaaa aaataggtgt actcgagact
      541 gaatacaaaa ggatgtacga ttttaagaaa tatgttttag atattgcatt aaagcaagta
      601 aatgaacata ccgatattaa tgtcaaagtt gaacaacata aaaccggcag atccattacg
      661 ggcttttcat ttagcttcaa acagaaaaag tcagcaacaa atacagccaa tgacattagt
      721 caggacaaag agctaaaaat caatttgact gatgcacaac gatatttatt tgctagtaaa
      781 ttatcagaac ttcctgagat ggctaaactt tcacaaggta atgaaagcta tgaacagttt
      841 gctgctcgga ttgtaaccat gctgcaagaa ccaaataaat taaaagaatt tatgccatta
      901 cttcgaaaag tcggctttca atgaatgatg tcaattgaca tagtaaagtt aacaatcaac
      961 tattaacact atttgaaaac tggtttacat aatgaaaaaa ctgtcagttt cagaactagc
     1021 gaaattatat ggatattcaa gacaagctgt atatgcccat ataaagaaag gaaatttatc
     1081 aaaaggatcg gatggattaa ttgacttttc agaagccttg agagtttttg gggaaccaca
     1141 aaaaagcaat tctagtgtca accaaagtca atcaactagt aggcataact taacagaagt
     1201 tgacttgcta aaacgtcaag ttgacatgct ggaaaaacaa ttaaatcagg caatacagag
     1261 agaaaatcaa tcattagaac gtgaatcgtt ttatcaagaa cagattgagg ccatgcagcg
     1321 cttactggaa gctccaaaag ccaatatgac tacgtttacc gatcatgaat ctgaacagga
     1381 tatagcaaca gattctcggc cacagactga atcgaactat gacggattga ctactccaga
     1441 gaacaaacgc atccctgttc cagaacatat tgagcctaca cctaaaaaga ggggcttcct
     1501 gagtcgtttt ttccttcctt acggttaacc aggactccac ttttttgatg agccaatttc
     1561 agtgcaagca catgaaataa tgactcctca aaaaaccgga tgatgtttat taaaactgaa
     1621 taaaatcgtg gaacattgct tatttattaa gcgtggaaca ctaaaaaaga taaaaaaagc
     1681 ccgaacaggg atgttcgggc tagaaactag aaactatagc attgatttct atagataatt
     1741 ataacgcatt tcgtataatc cgtattatgt taattttaga gattctacat aaacgtaaaa
     1801 tctcctgcgc ttagattctg gcatgtgttc catacttatc aatcataata gagctggctt
     1861 catttaagcc acgcacagtg acattcacac cattcttttg aaatttattt acaacagaat
     1921 caagcatggc gactgatgtt acatcccaaa tgtgagaatg ggtcaaatca ataatcacat
     1981 cctttacatc ctctttaaag tcaaaacctt gcatgaattt ttcagaagaa ctaaagaaga
     2041 tctgacctct caaatcatat aaacgggctg ttccttcaaa agatgcagtt acctggatat
     2101 cattttcaag tttattcgct aggaacaagg ctgaaagtaa tacgcctgtt aacacaccca
     2161 aagctaggtt atgcgtagca acaactacaa tcacagtcgc aatcataacg atattactgc
     2221 tcttaggatt atcttttaac tgagtgacag aactccattc aaaggtactg atagagacca
     2281 tgatcattac cgctaccaat gcagccattg gaatcacttt gagccagtca ctgataaata
     2341 caaccagaat aagaagaaaa atacccgcag agaatgttga taagcgtgta aggccacctg
     2401 attttacgtt aatcatagat tgaccaatca tcgcacagcc agctatcccc cccataaagc
     2461 cagaagcaat attcgcaatc ccttgtccct tacattcctg atatttatca cttggcgtat
     2521 ctgtcatttc atccacgata gttgctgtca tcatcgattc aagtaaacct acagccgcca
     2581 gtgcgactga ataaggcaaa ataatcatca atgtttcaat attcaaagga atatttggaa
     2641 tcaagaacat tggaagtgtg tcaggtaacg atcccatatc acccacagtt cgtacatcaa
     2701 ttcctagagc aattgccagt aatgtgattg caatgatgca gacaagtgga gaagggagta
     2761 attttcctaa ttggggaacc aaaggaaaga gataaataat cgccagacct aaagcaacca
     2821 gagcatatac atgccaggtc acattaatca attccggcaa ctgagccatg aaaattaaga
     2881 ttgctaacgc atttacgaat ccaataacta cagatttaga tacgaaccgc attaacttgg
     2941 cgagctttaa atagcctgcc aatatctgaa tcacgcctgt caaaattgtt gctgccagca
     3001 gatattcaag cccatgttct ttgaccaatg taaccataag cagtgccatg gcccctgtgg
     3061 cagctgaaat cattgctgga cggccaccta cgaaagaaat cacgacggca atacagaagg
     3121 atgcgtatag accaacttta ggatctacac ctgcaataat tgaaaatgcg atggcttcag
     3181 ggatgagtgc aagaccgacg actaatccgg caagtacatc cctacggatg ttagagaacc
     3241 actgttctcg gacattagat aacataaaca tagcctgttt tttaattttt cagataggct
     3301 agtgatgtga tatatacaac acacaagcga ctacaccaca tcactagaca gtgatgagtt
     3361 atttacagat tgaagacggt aaataaactt aaggtggcgt aagtgtcata agatgaagga
     3421 actttttaga gtaaaaaaat caggacaagg gtaacagcta ggtacttttt tataaagcct
     3481 ggatgttata tccaagcttt gtttaatcgg tattttatga agcttttagc tccccaagta
     3541 attgacctat ttccaaacgt gtatagccac gttctttaaa gatcaaaaca atagcctgtt
     3601 tataatcttc agaaatagcc gttttatatt tattaattaa ttgcagctca gcattaagct
     3661 gaaggtcatt tttgatcgtc aatacttccg tagagacttt attaaaaatc ttgaaattaa
     3721 acatacaggt gctcttcatc aatattgtag cggcccgact acaacaaaca cctgccttag
     3781 cttagctaga cacccttggg cgcaaggatt acaaatttaa aaagggtctg caagtttttc
     3841 tcaaggggaa gcatgaataa tccactaaat ttatatccat ttaacataat ggcgcttata
     3901 cgaagtacac ttgtatatta atataaatat caaatattta ataaaaatat aaaaatataa
     3961 aaatgcccaa aaagccgatt ttgaaacaag tcggcttttt tatgagcagt tttgatagtt
     4021 cttgccaata aatttgttta aaaagtagtc gattttgcga ttttttaaat tttatcaatg
     4081 cgtgaatgtt caaaattatc tccatttctt gtggataaat ttcaggcact ttgtacgctt
     4141 tggcttactc agattccagt tctttgcggc taccaggata gctagaatat ccctacgata
     4201 gattcataca ggaacaggat gttccggagc ataaaacaac aatgataagt tctaacacta
     4261 ggattgtgag gtagagcagc acctaccaaa gatcgcacaa taaaaaaccc cggcaggata
     4321 ccggggtttt ttattatcta aattttattt aggtttattt ttacttattt attgtctata
     4381 aacacaattt ctatgacctt agagatataa gtggaaatag atttaacaat gatttcattt
     4441 gacaaaaata ataagtaaag aataatccat actacctaat tttcagataa cttcacatga
     4501 tagatataaa taaaaaatct ttaaaaaaaa ttatatctat atgtacttta acaattttta
     4561 atattggatg cgtttcatta gaaccccaat caatagattc aaaaacacaa gataaaaaaa
     4621 taacaagtga aactatattc atagaagaaa aggattctat aaatcaactt ttgcaaaaag
     4681 taccagagcc gctagaagta aagattgtgg ggtatgtaaa taaagacctt cttcaaatgt
     4741 tttcttcctc aacccgttca gtagtggtta ataacgtaac tagaggaact agagtattaa
     4801 tttttgatac atcagggaac ctagccaata tttcagcaga tattgaatat ccttattggg
     4861 tagatttagc agggatatgt tttacggaaa attgttggaa gcaagaaaat acacctacag
     4921 tcaaatcatc agtaaataca cgtaggactt ctcccagcct aattacgcca cctaaatcct
     4981 ctactcaaaa taaaggaata agttcaaacc agggaaaagg ctatacaaac gtagatggaa
     5041 agtacgttcc aagtcctcgg aaagcaaata agcagccttc taatgcaaca gcaaagtgta
     5101 gagatgggac ttggtctttt agcttgaata ggagaggcac ttgttctggc catggtggag
     5161 tagctaactg gctttagaac ttagagtatt tttgatttaa agcgtcccat gcaacatatt
     5221 taaaacctta aaaaccaata gaaacaagaa ctttaagttc ctacttttca gctttgtata
     5281 acgtgtatta tgttaatttc aataaaatta aatcatcaat caggattatc tcttggtatt
     5341 ttttccaatt gatccacgaa taagacgaag cttttcgagt tttttaaaat agcagtatga
     5401 tttttacgct ttttcaaatc ttgactatgt tcaatatatt ctcgaacatt tttattaact
     5461 gtattaagaa ctttgctttt tcttcttaat gttgcagtta gctctggttc acacccagta
     5521 atcttacatt tcttttgccc atgataggct aaaagccaac attctaaaca atgcacagaa
     5581 atagcaaaga ttattttttc cttataatat tcatatgtat ctggttcaaa actatctatc
     5641 cattcgataa gtttgagctt gattctttca taaaactggt catgatcact atcagctaaa
     5701 ctaatagggc taacatcaaa attaggatgt tcgcaaacat ctgtatctat ctgaataact
     5761 aaataatcag tattaaccac agcatcttct aaatcttgag atttgagata actagcaact
     5821 aattcccaac caccgaaatt tccttcagca gtctttttat gtgtttcatc tagaaatggt
     5881 tgaataggtt gaaactctgc ttccccatta aagaacgact gaataacatg ctttaagaca
     5941 tgaaaatccg ttacaccttc acaaattaga ccaaatttca tcagtatttt ctcttagaaa
     6001 ttggtcggaa gaccacctaa caatccgcgc ataaaagctt ctgatagtct caatgtttca
     6061 ccattacgct ttggtttagg tagatcttca atgtttatac gttttaattg tgtcgctcca
     6121 tcatcatctc tttcaacttt aaacaaacgc tgttcatcat cttttaaatc caatccgtct
     6181 aaaattgcag gattgtgcgt tgtaacaaat acttgttttt tatttgcttt tgcaagctct
     6241 actaattttt taattaatac acgacaaagt ttaggattta gagatgcatc aatattatct
     6301 attgcaaaga agtcaggtgt atatttagaa ctaaataagg ccgcataaaa taagacaaat
     6361 aggaagccct cattggcact tctatgatca atttccctac ccataaatcg atctataatt
     6421 cttacttttt gttctgatga tgattgatca tctacaataa ttttttcaac ccattgaaac
     6481 atttctaatg tttcacaaac ctgtaaaaag ctttcttgtt catgttgctg cataacttga
     6541 agtaacttaa acaaaccttc tccattgatt cctaatggct ctatttgaga ttcactagag
     6601 aaattacgta aagaacttaa ttcaggtgaa aaaataacaa aattggactc tttggaagtt
     6661 tcattcttag aataatattt gtcgaaaaca gttctctgga tagcacccaa cgtattaata
     6721 taatcgacca tattcgatac catctttatg tcattcttac tatctttttc ttttttctct
     6781 gctgaatctc taatttcatt caaatttttt aaaagactct ctaccgaacc gtatgtagtt
     6841 tcagcaaggt ctttaatttt atctttcaaa aaattgctgt tgaaatcttt gatatcaact
     6901 atttctaaat catcatttag atcaagattt cgatgcaaaa tcagtagatc tgattccaat
     6961 tgagcaaatg gttcatcttt attaaatttt atgttaaata cacatccata tccattttca
     7021 tagacatttg taatatttat attgtcagcc atattattgt taaaacaaga gaataagcta
     7081 tcaggattaa ctattcttat tcctcttgaa agcataaact cattactaaa cttattggca
     7141 ataattgcag agccagatgc taaaacttca agaaagttac tttttccagc cccattttcg
     7201 ccaattaaaa cattaaattg gcctacgcca acttctagtt ctttaataga tttatagttg
     7261 tgaattttaa tcccagctaa cattgagtac ctaaataact taatctggat tgaagaatta
     7321 agatcttatc atcaatgaaa ctaagtatcg tagatactta attcatttaa cataaaaact
     7381 cttatgcgaa gtcaaaaaag tagccccata cccttattgg ataggagcat tgtggcaaaa
     7441 aatagagtga attcgataaa aaatgaaatc ctctaattct tgttttaatt atttttttaa
     7501 ctaagaatta gaggattaat ttctttacct ctggagtttt ataattattg ttataagatt
     7561 tattaaatca cttattgctc aaataatctt cacaatctga tcattcgctc atggataagt
     7621 gtgatggaaa tcacaaaatc aatcagatct aaaaatcaca caattacact atttatgatg
     7681 agcgaattgt tcttttaatt ttctgatcag tcttgtattg cgacaaagca tagaccgccc
     7741 agatggcagc cgggatccaa cctaaaactg taatctgaag tagcaaacaa atgatgcctg
     7801 caaatggtcg ccctatagtg aaaaacagca gccagggaaa aattaaagca attagcaatc
     7861 tcatttcaaa ttcctatgta tcttttcaaa aaattaataa ttaaatgcgg gcagatcaaa
     7921 cagcttacga tctctttcat ccattaaata tttatccatc tgagcaatca gttttgctct
     7981 ggcttgttca tcttcttcgg taattttcaa catgcaggat ccgattaaaa tcttgcgtct
     8041 agtatcatct ttacgagtct gttgtttctc tttagcacgt tctcttgcta gtgctgcctg
     8101 tctttgagcc tttaactgtt ttagcttttc ttcctgtgcc ttaatcttgc tgtctagtgt
     8161 ttcagtaata ctcattaatc caataccatc atcctgggaa accatcccta acataagcgc
     8221 acttgaatat gcattcaagg tgctatatgt atagtaatgt tttcctcgaa gagcgcactt
     8281 atgcaaactt cgtttgcaag tccgattggg gagaccccaa taccccgaca accgtaggca
     8341 cggttgtatt tcactatgtg aaaatttaaa agcgaaaaac aggcatggcg atttaccact
     8401 tttcagtcaa aaccgtagca cgatcagcag ggcgttctgc cactgctgca atcgcctatc
     8461 gggctggtga aaagatttat tgtgaacgtg aaggccgaga acatgactac agccgaaaaa
     8521 ctggcgtgga atataaagaa atttatttac ccgaagacgc accaaagcat ctaaaaaaca
     8581 gggaaaaact ctggaacgaa gttgaacaac gggaaacccg aaaaaactcg acagttgccc
     8641 gggaatttga aatcgctttt ccaagtgaat taaatcagga acaacgcctg gccatgctcg
     8701 aagaactatg cgccagtatc gtagacagac atcaggtggc cgtcgatgcc tgtatccatg
     8761 caccgcatac tggatccggc agcgatgaac gcaattatca cgcgcatatc ctgatgagca
     8821 cacgtaagct cacgcctgaa ggttttaccg agaaaacccg ggaactggat cagaaacaca
     8881 gtggagaaat cgaacactgg cgcgaacact ttgccgatat ctgcaatatg cacctagatc
     8941 tggccggata caccgcccga gtcgaccacc gcagctacaa agaccaggag aatggcctgg
     9001 aagccaccct gcacgaaggg ccgaaagtca ccgaactgcg ccgaaaaggg attgagaccg
     9061 aaatcagccg aagtaatgac gaaatcaaac agagaaatca ggctcagttg caatacggca
     9121 aaaatatgga tctgctgatt gctgaaaatg agataaaact cagcacactg aaaactgaac
     9181 agcagatcca aatcaaaaat agcgctaaaa cgccaccaat tgacgaaaaa gccctgtttg
     9241 aggaaaaaca gagggaaacc cttggcaaag tgctaaaacg cgaaatcagc gcaaaagacg
     9301 cgaatctaga cctggatttc atgcagcagc atctagagaa ggccgaaaag accctgagca
     9361 agcataacaa acatcaaaac gagttcaatc agcagctcgc ccaggagatc gtgaaaaacg
     9421 gactcaagca aagtcatgac aaattgcaaa gcctggtcga tcaacacaac gaattaaccc
     9481 agaacaaacc cttactgttt ggaaaaaaag cctgggaagc ccaacgtgac gagatttatc
     9541 aggaacacaa aaagctgaaa ggtcagcatg aacaccagaa aaaacatggt gtgaaggatt
     9601 tattggacaa tgaaaagttc aaagaacatg cctggaagca gtaccaacaa caacatccgg
     9661 ccaaagccaa acaataccag actctatacc catcctatca agtcattaaa aaatgtgtga
     9721 atgagatcaa agcagaacaa cagatgaaac tcagacaaga acaacaactc aaagcacagc
     9781 aacatgcgcc taaactgaaa tctcgtggca tgagtcgcta aacagagcga gtgtctacga
     9841 gcgaactgag aaaatttgcc catccctttc cctgattaca agctccctat ccatcaatca
     9901 cctgcatggc agagcatccc gtatgggtgc gaactttcca agtttgattc ctaaaccgag
     9961 cgtagcgaga aaaaaaagct gatgagcgaa gcgaattccg agttgctttt gctttttcta
    10021 aaagtcacga aaatatttac caaaaattgc cccgacgaat cgagcgaaag cgagattcaa
    10081 tagagtttga gcgaagcgaa aaccaagggc aatttttcat gaaatatgct tttaattatt
    10141 tttaagcttt taaatgcttt tagatagcct gtaaacctga atttataagg ctttgagaga
    10201 ttataaaacg aggtttacct tgcattaaaa cgaggtttac cttgcattaa aacgaggttt
    10261 accttgcatt aaaacgaggt ttaccttgca ttaagcgagt tgataatata acctcgtctt
    10321 attgacttaa aaatataaat ttttcat
//
