LOCUS       NZ_CP032105            12284 bp    DNA     circular CON 31-MAR-2024
DEFINITION  Acinetobacter lwoffii strain EK30A plasmid pALWEK1.2, complete
            sequence.
ACCESSION   NZ_CP032105
VERSION     NZ_CP032105.2
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN05251105
            Assembly: GCF_019787625.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter lwoffii
  ORGANISM  Acinetobacter lwoffii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter.
REFERENCE   1  (bases 1 to 12284)
  AUTHORS   Rakitin,A.L., Ermakova,A.Y., Beletsky,A.V., Petrova,M.,
            Mardanov,A.V. and Ravin,N.V.
  TITLE     Genome analysis of Acinetobacter lwoffii strains isolated from
            permafrost soils aged from 15 thousand to 1.8 million years revealed
            their close relationships with present-day clinical isolates
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12284)
  AUTHORS   Rakitin,A.L., Ermakova,A.Y., Beletsky,A.V., Petrova,M.,
            Mardanov,A.V. and Ravin,N.V.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2021) Institute of Bioengineering, Research Center
            of Biotechnology of the Russian Academy of Sciences, Moscow 119071,
            Russia
  REMARK    Nucleotide sequence updated by submitter
REFERENCE   3  (bases 1 to 12284)
  AUTHORS   Beletsky,A., Kurakov,A., Petrova,M. and Mardanov,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2018) Lab of molecular cloning, Institute of
            Bioengineering, Research Center of Biotechnology of RAS, Prosp.
            60-let Oktyabrya, bld 7-1, Moscow 117312, Russia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Newbler v. 3.0
            Expected Final Version            :: yes
            Genome Coverage                   :: 25.0x
            Sequencing Technology             :: 454
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_019787625.1-RS_2024_03_31
            Annotation Date                   :: 03/31/2024 06:12:30
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,448
            CDSs (total)                      :: 3,336
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 112
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 222
            CDSs (without protein)            :: 222
            Pseudo Genes (ambiguous residues) :: 0 of 222
            Pseudo Genes (frameshifted)       :: 80 of 222
            Pseudo Genes (incomplete)         :: 138 of 222
            Pseudo Genes (internal stop)      :: 46 of 222
            Pseudo Genes (multiple problems)  :: 39 of 222
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP032105.2.
            On Aug 29, 2021 this sequence version replaced NZ_CP032105.1.
            Bacteria and source DNA available from Dr. Petrova M.A.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..12284
                     /organism="Acinetobacter lwoffii"
                     /mol_type="genomic DNA"
                     /strain="EK30A"
                     /isolation_source="permafrost, Kolyma lowland"
                     /db_xref="taxon:28090"
                     /plasmid="pALWEK1.2"
                     /geo_loc_name="Russia"
     gene            join(11622..12284,1..1155)
                     /locus_tag="ABEKA_RS01195"
                     /old_locus_tag="ABEKA_3268"
     CDS             join(11622..12284,1..1155)
                     /locus_tag="ABEKA_RS01195"
                     /old_locus_tag="ABEKA_3268"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="WP_223287773.1"
                     /translation="MNNFAKSALVTSLFTLITACGSSDNDDKLVSQPSIEPIPFEPLEA
                     TISDLHVSLKSNGSNCVNIVQSYLDRINAYDKQGPTLNSVISVNPNILKEAVELDEYYK
                     KTGKFKGDLHCVTVLAKDNIDVINMANTAGSDALLDNVPQDDAYIIKNIKEQGGLIIGK
                     ANLDEFAFGFGGKSTVGGQAKNVYDLTKGPGGSSAGTGTAISASLARVGIGTDTGGAKN
                     VYDLTKGPGGSSAGTGTAISASLAMVGIGTDTGGSIRVPSAVQGLVGLRPSLRILSLDG
                     IIPLAPTQDTAGPMCRQSIDCAKLFTAMVGYDPSSSSNQRSDFAHDAPLLNSETAYRAL
                     INQPENYVPINASLAGKHIGLVRNVYANSEEGLLVQDVITKAADKMREAGAIVEEVEIS
                     DLSTILGSSDITSNLGYTGRFASLSSSEFKQSLTDYLATATTAYKSYIDLLNSGKMIQS
                     FKSYDNDSTSVEFINRYHLNTVVRAPFVRLRLSSALENSNLNGISKGQRFDALAYPSIT
                     GLTNNIPSSPTTGSNNRMSPFSGFPALSVPAGTVNYEKRSVYPMNVNIEFIAREFDEPT
                     LFEIATAFEKVNPVRMIPTHTPALTTKLY"
                     /name="amidase family protein"
     gene            complement(1349..1867)
                     /locus_tag="ABEKA_RS01200"
     CDS             complement(1349..1867)
                     /locus_tag="ABEKA_RS01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication DNA-binding protein"
                     /protein_id="WP_159373190.1"
                     /translation="MKTLTVLELSKLYNINRQTIYNNIKKGILSKNSDNKIDLAEAIRV
                     FGEPNKKHDVKESVKVDSPNSAEVLLLRQQIDMLKNQLDDAKDRESFYQNQIETMQRLL
                     EAPKPHITQSGDHGSVQPEPATEAKEEVIAMPPKEPEVIENKRIPVPEHVEPEPEKRGF
                     WSRFFKPYG"
                     /name="plasmid replication DNA-binding protein"
     gene            complement(1989..2987)
                     /locus_tag="ABEKA_RS01205"
                     /old_locus_tag="ABEKA_3270"
     CDS             complement(1989..2987)
                     /locus_tag="ABEKA_RS01205"
                     /old_locus_tag="ABEKA_3270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004779643.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_026056584.1"
                     /translation="MAISLKKIKNFEEHDKSFFPLSAPAKRYPKDFAVFQNIFWQLKHE
                     RSPKDDILKLLFLFSPKVRLASDPNETTEFTVTAKEYAELTGLKLNSAYVALERVVDAL
                     YEHSVIFYHPEKDREIRTRLISTCSYKDGCFYIGFTHFALYIMYVFNKQNSYTKFKVKS
                     AVALHGHGLKLYPFLIQNEFRSNFDVSIRELKSALGLAENSYPEYRDFKTTILKPHIDA
                     INAKTELTVEFKALKKEGRKASHVNFIVRKKKTIKTEQQDEIGNNQIRSKVTAKMVYLT
                     IIQSEELSLRLKQSNETTDELVERVKNDFKNNEGSRWIEKLAEFNVRFETH"
                     /name="replication initiation protein"
     gene            complement(3840..4991)
                     /locus_tag="ABEKA_RS01210"
                     /old_locus_tag="ABEKA_3272"
     CDS             complement(3840..4991)
                     /locus_tag="ABEKA_RS01210"
                     /old_locus_tag="ABEKA_3272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017397930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_159373191.1"
                     /translation="MAESNETIDLLTAIVGTIESSDEKAQIAKSELLEQLKLLTSKTDV
                     ISTNFPKLIETLSNFDIKIASSFSNIENIDKHLTTINNKISKAEIAVFNIQEQSKNVQL
                     DHIIETLSSADQKAQQLSNHLRNSSTKYAENMSAATNIALKQIESLKEEVANIANTFSE
                     IVSTTIADRIVTDVPPRLSNYFFREAAAGIDTGAKEVARTLTDEAFKNIDSTLKHTEKT
                     ILSDLQLFKKESDSIHQNWMQNFKKQQAEAEAITKESHTMHMQREKAYQQHVSQEKAVV
                     KRNWLLIFLIVWFGFTGTAVGIAYAVKSASEKTVDDALNYLALRNDLTQLGFSVENKEF
                     CQSLLPKKQFNFTQGPQVSSCFYLSSPDNEYTVNNRNLMIFTK"
                     /name="hypothetical protein"
     gene            complement(5014..6237)
                     /locus_tag="ABEKA_RS01215"
                     /old_locus_tag="ABEKA_3273"
     CDS             complement(5014..6237)
                     /locus_tag="ABEKA_RS01215"
                     /old_locus_tag="ABEKA_3273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005102330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_159373192.1"
                     /translation="MSKIIIRNQYSCRISAIKKSKGHSPLRAIAYIQGRKMQNSLSGDE
                     HNFSNKKGVIDTGFLMPLGIKTGMNEEQFYNHLENNSHASTNILAYSSIMSLPAELDIK
                     NQKKLVEDFCKYFTDTYQTAVSFAIHEADNHKRKKAKLEEIEKHNLPSTDDDLRMQMQN
                     NHVHLVIPYCKISALTESDLTAKRKKKAIADMFKLGNQVKDFNPIKFGSEIKKDNPDLM
                     NTKQNFLQFMRQEWCRFINNSLELNNRIERYTHKSYKDLGLQISATVHEGEVRKNILAS
                     GQKTAMNEYNKKQKAKQKTEADYLNFINVPLPAELAHKILSGQKLAEKELINSLKPPTQ
                     TAEDPKPNQPNLREKLDRLKKSTISPGTTPGHESLQQKLERLKQKQVEQPRSIAEPKHD
                     NDNDYSPF"
                     /name="MobA/MobL family protein"
     gene            complement(6984..7229)
                     /locus_tag="ABEKA_RS01220"
                     /old_locus_tag="ABEKA_3276"
     CDS             complement(6984..7229)
                     /locus_tag="ABEKA_RS01220"
                     /old_locus_tag="ABEKA_3276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005249014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004282220.1"
                     /translation="MYSYPDSNTEKKIALMIINDFFIQKAHELWIFLQLDQCFNDYEAT
                     LIWTRRYLEKHPECEYSDIQKAFVSCFPEHFFSFDC"
                     /name="hypothetical protein"
     gene            7383..7607
                     /locus_tag="ABEKA_RS01225"
                     /old_locus_tag="ABEKA_3277"
     CDS             7383..7607
                     /locus_tag="ABEKA_RS01225"
                     /old_locus_tag="ABEKA_3277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004676219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004758944.1"
                     /translation="MPYTEFQRLVGKAGLSIKEFAALLDMKPNSITNYSKQGVVPTHIA
                     VIVALISTMKDEGLDFYPIFEKVKSYSQE"
                     /name="hypothetical protein"
     gene            complement(7895..9700)
                     /locus_tag="ABEKA_RS01230"
                     /old_locus_tag="ABEKA_3278"
     CDS             complement(7895..9700)
                     /locus_tag="ABEKA_RS01230"
                     /old_locus_tag="ABEKA_3278"
                     /inference="COORDINATES: protein motif:HMM:NF024700.4"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA];
                     GO:0016887 - ATP hydrolysis activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="WP_159373193.1"
                     /translation="MYLRNLHIRNIGPIEKLDLEFELQENFNPKPIILVGKNGSGKTYT
                     LSYIADSFYELAKKQFNDIVKKSNNLDNPYFRVISGKDISTSNNSKSASAYLRFIDDQS
                     NNEIHYCEKIGEISFTQELLNLYGNKITTFKSDNNPDKNILASEEVIKKLFLNTITFFP
                     SFRKVTPHWLNLNAQNQDLFSIEVKYGGNLNKEIMCVDTFQKNIQWMLDVILDSAILPA
                     ELDHIKSEDDRNQEIRNKQAINHSYRNINKIFQTVLENDEIYLKTGWRNSAESRLSIHC
                     RGKVVVPTLEQLSTGQLILLNLFITIIRHADYKDMNNSIRLENIEGIVVIDEIDTHLHS
                     KHQSDVLPKLISLFPKIQYIVTSHSPLFVLGIENKLGAENIQIIDLPSGINITSERFNE
                     FEESFNIYKETAKFEENLHKKIQETLKPLILVEGKTDIKFLKKAFNLKEKNEWLENIDI
                     DEIGITQENGQVKFGGESSLNKGFEFLKHNLTAINRKVLFLYDCDTNISRQTLGNITQD
                     KVPCNLDNTKIKKGSENLLNMELFTEEFYVTREKQDEYGCLQKIQNFEKNKFCDYICQN
                     INDPKVFDKYEELVFPIIETFLKET"
                     /name="AAA family ATPase"
     gene            9906..10226
                     /locus_tag="ABEKA_RS01235"
                     /old_locus_tag="ABEKA_3279"
     CDS             9906..10226
                     /locus_tag="ABEKA_RS01235"
                     /old_locus_tag="ABEKA_3279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_005404023.1"
                     /translation="MLFIETSIFTKQIKELVNDEEYRQLQQDLLVQPDKGDLIKNGGGI
                     RKVRCAQGNKGKSGGIRVIYYWVTEDDQIFFLVAYPKSVKDNLTDKETSILRQLVKEQF
                     HG"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            10219..10491
                     /gene="nadS"
                     /locus_tag="ABEKA_RS01240"
                     /old_locus_tag="ABEKA_3280"
     CDS             10219..10491
                     /gene="nadS"
                     /locus_tag="ABEKA_RS01240"
                     /old_locus_tag="ABEKA_3280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000369784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
                     /protein_id="WP_004896911.1"
                     /translation="MDNNLFDDLVASIKEAGAIKRNEMKASRVTELELPDIKEVREKTG
                     LTQAEFAARLHISARTLQNWEQGRRYPTGPAATLIRILDAHPTLI"
                     /name="nadS"
     Typing          complement(1990..2987)
                     /name="rep_cluster_1041"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.8"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aatgtttatg atttaacaaa aggccctggt ggttcatcag caggtacagg aactgctatt
       61 tctgctagtt tagctatggt agggattggt acggatacag gtggctctat ccgtgtccca
      121 tcagcagttc agggcttagt tggtttacgt ccttcactac gtattcttag tttagacggc
      181 attattccac tggcaccaac acaagataca gctggtccta tgtgtcgtca gagtattgat
      241 tgcgcaaaat tatttacagc tatggttgga tatgacccat catctagctc aaatcaacgt
      301 agtgattttg cacatgatgc accattatta aattctgaga cagcttatcg agctttaata
      361 aatcagcctg aaaattatgt tccaataaat gcttcgttag caggtaaaca tattggttta
      421 gtaagaaatg tttatgctaa tagtgaagaa ggtcttctag ttcaagacgt gattacaaaa
      481 gctgctgata aaatgcgtga agcaggtgca atagttgaag aagtagagat atctgatctc
      541 agtactattt taggaagttc tgatattaca agtaatttag gttatacagg gcgttttgcg
      601 agtctttcaa gttctgaatt caagcagtct ttaacagatt atttagcaac agctactaca
      661 gcttataaat cctatatcga cctattgaat agtggaaaaa tgattcaatc ttttaaaagt
      721 tatgataatg attcaaccag tgtagagttt attaatcgat atcacttaaa cactgtagta
      781 cgtgcacctt ttgtgcgttt gcgtttaagc tcagcattag aaaatagtaa cttaaatggt
      841 atatctaaag gtcaaagatt cgatgctttg gcatacccat caattactgg tttaacgaat
      901 aatattcctt ctagcccaac gactggaagt aataaccgta tgagcccatt ttctggattc
      961 cctgcattat cagtaccagc aggtacagta aactatgaaa agcgtagtgt ttatccaatg
     1021 aacgttaata ttgaatttat tgcaagagaa tttgatgaac caacgttatt tgagatcgcc
     1081 acagcctttg aaaaagtaaa tcccgtaaga atgataccaa cacatacccc tgcacttaca
     1141 accaaattat attaatttaa aacttcctaa aattaacata atacacctta tacgaaatta
     1201 ttttaattta tttataaaat tcagtatctt atgttgatgt gaaaagccca actcagctga
     1261 gttgggcttt tttgtcgatt cttcacgttc cacgcttact taatgatcaa tgttccacgg
     1321 ttttaataag cccaggtgaa caatcccatc agccatacgg cttaaagaag cggctccaga
     1381 agcctctttt ttccggttca ggctctacgt gttcaggaac tggaatgcgc ttgttctcga
     1441 ttacttcagg ttctttaggt ggcattgcta taacctcttc ttttgcttct gtcgctggtt
     1501 ctggctgtac cgatccatga tcaccagatt gagtgatatg gggttttggt gcttccaata
     1561 gacgttgcat cgtttcaatt tgattctgat aaaaagactc gcggtctttg gcatcatcaa
     1621 gctgattttt cagcatgtct atctgctgtc taagcagtaa aacttctgcc gaatttggac
     1681 tgtctacttt tacagactct tttacatcat gttttttatt aggctcccca aatacacgga
     1741 tagcttcggc tagatcaatc ttattatcag aatttttgct taaaattcct ttctttatgt
     1801 tgttgtaaat agtttgtcta ttaatattgt aaagcttgga aagttcaaga accgtaaggg
     1861 ttttcatcct gtaaacctat gcctaaactt gtaaaaagtt agtgtctatt agactgtcta
     1921 atagacaaat gatcaagttt aaattttaag caaggtaaat gataattttt aaaataaatt
     1981 atgatgtttt agtgagtttc aaaccgcaca ttaaattccg ctaatttttc tatccatcta
     2041 ctaccttcat tattcttaaa atcatttttt actcgttcga ctaattcatc tgttgtctca
     2101 ttggattgtt taagccgtaa acttaattct tcactttgaa ttatcgttaa gtaaaccatt
     2161 tttgcagtga ctttagatcg tatttggtta ttaccgattt catcttgttg ttctgttttt
     2221 atagtcttct tttttctcac tataaaatta acatgactag ctttacgtcc ctctttttta
     2281 agagctttaa attcaacagt aagttctgtt ttcgcgttaa ttgcatcaat gtgaggttta
     2341 agaatagtag ttttaaaatc tcggtactca gggtaggaat tttcagcaag gcctaaagca
     2401 ctttttagtt ccctaatgga aacatcaaaa ttagagcgaa attcattttg tattaggaat
     2461 ggatagagtt ttaagccatg gccatgtaag gcaactgctg atttcacttt gaattttgta
     2521 tacgaatttt gcttgttaaa aacatacata atgtataggg caaagtgcgt gaaaccaata
     2581 taaaagcatc catccttata tgagcatgta ctaatgagtc ttgtacgaat ttctctatct
     2641 ttttcaggat gataaaaaat aacactatgt tcataaagag catctacgac tctctctaat
     2701 gctacataag cactgtttag ctttaatcct gttaattccg catattcctt agctgtcaca
     2761 gtaaattcag ttgtttcatt tgggtctgat gctagtcgaa cttttggact gaataagaac
     2821 agtaatttta aaatatcatc tttaggagac cgctcatgtt ttaattgcca gaaaatattt
     2881 tgaaagacag caaaatcttt tgggtaacgt ttagctggtg ctgaaagagg aaaaaaagat
     2941 ttgtcatgtt cctcaaaatt cttaattttc tttaatgata tagccatgta gaaggagtca
     3001 ctattattac tattatgtgg gggttgcttg ataataatta cccccacttt ataagtcaag
     3061 taattcatat aacctaacaa tccaccccca ccaacctaac aatccacccc caccaaccta
     3121 acaatccacc cccaccaacc taacaatcca cccccaccaa cctaacaatc cacccccact
     3181 ttaagccaaa aacctaacaa tccaccccca ctttaagcca aaaacctaac aatccacccc
     3241 cactttgatg tctaaaagcc ttatctatca atacattcat actatccgta aagcttttta
     3301 aaagcttttt aaaagctatt aaaagctttt aataaagctt ttaaaataaa tctaaataaa
     3361 aattgccctt ggttttcgct atgctcaaac tctattgaat ctcgctttcg ctcgattctg
     3421 aggggcaatt ttttgcttaa ttcagttgta aattaaagga aaaacaaaag ctccatcgtt
     3481 cgcgcacttc gtgacgctct tcatctcccg tttagaccaa taatgggagc ctgtaatcag
     3541 ggaaaaagtg gcggcaaaat tcacttacaa ctgttcgctc gtagacactc gctctgtaac
     3601 ttgttgttat ttttttaaat attcttaata acagtgagat taatagtaat tttttgattt
     3661 attaacggca ttagttgaaa cacaggatga atatagcttt atttcttcac catttgagcc
     3721 acattgagtt tttattgttc ttcggtgccc aattaccgtt tttatttctt tgttcaatct
     3781 gggcgattct gtgtgctttt agagattcaa aagtaagccc tcaaatgagg gcttgattct
     3841 tattttgtga aaatcattag atttctattg ttcacagtat attcattgtc cggactgctt
     3901 aaataaaaac aactagatac ttgaggacct tgggtaaaat tgaactgttt ttttggcaat
     3961 aggctttgac aaaactcttt gttttctact gaaaagccga gttgggtcag atcatttctt
     4021 aatgccagat agtttagagc atcatctact gtcttttcag atgcactttt tactgcataa
     4081 gcgatgccca ctgctgttcc agtaaagcca aaccacacaa ttagaaaaat taatagccaa
     4141 ttgcgtttta caacggcttt ctcttggctg acatgttgtt gatatgcttt ttctcgttgc
     4201 atgtgcattg tgtgcgattc ttttgttatt gcttctgctt ctgcttgttg ttttttaaag
     4261 ttttgcatcc aattctgatg tattgaatcg ctttcttttt tgaataactg gaggtcgctt
     4321 aagattgttt tttcagtgtg cttgagcgtt gaatcaatat ttttaaatgc ctcgtctgtt
     4381 aaagttctag ctacttcttt cgcacctgtg tcgattccag cagcggcttc tctaaaaaaa
     4441 tagttgctca atcttggtgg aacgtcagtg acgatacgat cagcgattgt tgtcgaaacg
     4501 atctcagaga aagtatttgc aatgtttgcg acttcttctt tcagtgattc gatttgcttt
     4561 agtgcaatgt tcgttgctgc actcatattt tctgcgtatt tcgtacttga attacgcaaa
     4621 tgattgctta gctgctgagc tttttgatct gcactactaa gcgtttcaat gatgtgatct
     4681 aactgcacat ttttcgattg ttcctgaata ttgaaaacag caatttcggc tttactgatt
     4741 ttattgttaa ttgttgtcaa gtgcttgtct atgttctcga tattgctaaa acttgatgca
     4801 atcttgatgt cgaaatttga tagcgtttcg atcaatttcg gaaaatttgt gctgattaca
     4861 tcagtctttg atgtaagcaa tttcaattgt tctagtagct ctgatttagc tatttgtgct
     4921 ttttcatctg agctttcaat cgtgccgaca atcgctgtca gtagatcaat agtttcgtta
     4981 ctttcagcca tctgactgtt cctttattaa taattaaaac ggtgaataat cgttgtcgtt
     5041 gtcgtgtttc ggctctgcaa ttgacctcgg ttgttcaact tgcttttgct ttaaacgctc
     5101 tagcttttgt tgcaatgatt cgtgtccggg tgtagtacca ggggagatgg tggatttttt
     5161 tagacgatct aatttttcac gtagattagg ttggttaggc tttggatctt ctgctgtttg
     5221 ggttggtggt ttgagactgt ttattaattc tttttcagca agcttttgac cgctcaggat
     5281 tttatgagca agttccgcag gtaatggcac attgataaaa ttgagataat cggcttctgt
     5341 tttttgtttt gctttttgct ttttgttgta ttcattcata gccgtttttt gaccgcttgc
     5401 aagaatgttt tttcgtactt caccttcatg aaccgtggcc gaaatttgca agcctaaatc
     5461 tttatatgat ttatgcgtat agcgttcgat tcggttgttt agctctaatg agttattaat
     5521 aaatctgcac cactcttgcc gcatgaattg caagaaattt tgctttgtat tcattaaatc
     5581 cgggttgtct ttcttgattt ctgagccaaa tttgattgga ttaaaatctt taacctgatt
     5641 gcccaattta aacatgtcag caatcgcttt tttcttacgt ttggctgtta agtcgctttc
     5701 tgtgagtgct gaaattttgc aataaggaat cactaaatgg acatgattat tctgcatttg
     5761 catacgcaaa tcgtcatctg tggaaggtaa attatgcttt tcgatttcct ctagttttgc
     5821 tttttttctt ttgtggttat ctgcttcgtg aatggcaaaa ctaacggctg tttgatatgt
     5881 atctgtgaaa tatttgcaaa aatcttcgac taattttttt tgattcttta tgtctaattc
     5941 cgcaggtaat gacatgattg atgaataggc aagaatgttt gttgatgcat gagagttgtt
     6001 ttctaaatga ttataaaatt gttcttcgtt catgcctgtt ttaatgccaa gcggcattaa
     6061 aaaaccagta tctattacgc cttttttatt agaaaaatta tgttcgtcac cactcaaact
     6121 attctgcatt tttcgtcctt gaatatacgc aatcgctcga agtggactat gacctttgga
     6181 ttttttaatt gcagaaattc gacaagaata ttgatttcga ataatgattt tagacatggc
     6241 ttggcgcttt tgctttgtac ttttaaaacc ttagttgatc taaaaaaatt ggcaaagcac
     6301 aatgattttt agatcagaaa aaaggtttaa aagtcccccc ctttccctag tttttttggg
     6361 gggcgcacga ctcaaaattg atatgtagcg gagcggaatg ttaattttgg gttaaagtgc
     6421 gcttcattct acgtcatttc tacgttttta cagtctacgc ttgcgcttga ctgtaaaaac
     6481 gtgaaatgtc gtgaatgaag ttgtcacgta attcgactat acactacaaa aaaaactgac
     6541 ctgtatttgg tgttattttt cgattttttt cacttgtgat aatttcaatt aattgttttt
     6601 tccgtgatag taatatgaat attgattact ctaaatgtgt cttgatgaat actaagacaa
     6661 aatctttttt acagtatcat ttcaataatg agaaagtgaa agacttaggg actcaaatca
     6721 aattttatgc atcatttaac tgacttatcg tattttataa gtttttattt ttgtatcttt
     6781 aaagtacacc ctaaagtaac gtcatggcgg ccccaatcct gaacctaaaa tcaatgtctt
     6841 agatgccgtg gaacatcaat tttagtatgc tcaaagaaag tcttagattt atacgtgaca
     6901 ataaaaagcc agttgtatga actggctttt tctatttggg atttacttac ccattgcgat
     6961 ttttttaccg tgcctctcat aaattaacag tcaaagctga agaagtgttc tgggaaacaa
     7021 gaaacaaagg ctttttgaat atcgctgtac tcacactcag gatgcttctc taaataacgt
     7081 cgagtccaaa tcagtgtggc ttcataatcg ttaaagcact gatcgagttg taaaaaaatc
     7141 cacagttcat gggctttttg aatgaaaaag tcattgatga tcatgagagc aattttcttt
     7201 tcagtgtttg aatccgggta tgagtacata ttacatttct ccgatagtaa ggataaatag
     7261 gttagttgat aagctattta taacacataa aaatatgtgt ttaccacata attttatgtg
     7321 tttttatgta aatataaccc tatgctataa tctcaaaaca ccatttctat aaatttaatt
     7381 aaatgccata tactgaattt caacgtcttg tcggtaaagc agggctttca attaaggaat
     7441 ttgctgcctt attggatatg aaacctaatt cgattacgaa ttacagtaaa caaggtgtgg
     7501 ttcctacaca tattgctgta atcgttgcgt tgatttctac gatgaaagat gagggattag
     7561 atttctatcc aatttttgaa aaagttaagt cgtattcaca agaataaaac tctcgttttt
     7621 tgattgttgt aagcttttta gtagatattg atgatgtaaa aaatgcctga acttcaggca
     7681 tttttttgtt tcagggactt ataagcctcg atagaacttt tcaataaagt atcgagtttt
     7741 gagaaccagg gtgatcatca aattcttatg ttctgagatc ttaggtcgag caatcgataa
     7801 accggacatt tctatttggg ggttacaact gaatttcgca taagagattt tatgttaaat
     7861 aagaacacac ctttaaaagg tgtctaatag atatttaagt ttctttcaga aaagtttcga
     7921 taattgggaa gactaactct tcatatttat caaagacttt agggtcattt atattttgac
     7981 aaatataatc gcaaaattta tttttttcaa aattttgaat tttttgtaag catccatact
     8041 catcttgctt ttctcttgtc acataaaact cttcagtaaa aagctccata tttagaagat
     8101 tctctgaacc ttttttaatt tttgtattat ctaaattaca agggactttg tcttgtgtga
     8161 tatttccaag ggtctgccta ctaatgttag tatcacaatc atataaaaat aagaccttac
     8221 gatttattgc cgtcaaattg tgttttaaaa actcaaaccc tttgtttaaa ctactttccc
     8281 caccaaattt aacttgtccg ttttcttgtg taataccgat ttcatcaata tctatatttt
     8341 ccaaccattc atttttttct tttaagttaa aagctttttt taaaaactta atatctgttt
     8401 ttccttcaac aagtataagt ggctttaatg tttcttgaat ttttttatgt aaattctctt
     8461 caaatttagc tgtttcttta taaatattaa atgattcttc gaattcatta aatcgttctg
     8521 aagtaatatt tattcctgaa ggaagatcaa ttatttgaat attttcagct cccaatttat
     8581 tctctatacc taaaacaaaa agtggtgaat gtgaggtaac gatatactgt attttcggaa
     8641 aaagacttat aagcttaggc aaaacatcac tttgatgttt actatgtaag tgtgtgtcaa
     8701 tttcatcaat aacaactatt ccttctatat tttctaagcg gatactatta ttcatatctt
     8761 tataatccgc atgacgtatt attgtaataa acaagttaag taaaatcagt tggcctgtag
     8821 aaagttgctc taaagttggt acaacaactt ttcctctaca atgtattgac aatcgagatt
     8881 ctgccgaatt tcgccatccc gtttttagat atatttcatc attttctaaa actgtttgaa
     8941 aaattttatt gatatttctg tatgaatggt taatagcttg tttatttcta atttcttgat
     9001 ttctatcatc ttcacttttt atgtgatcta gctcagcagg taaaatagcg gaatctaaaa
     9061 taacatctag catccactga atattttttt gaaaagtatc aacgcacatg atttctttat
     9121 ttagatttcc accatatttt acttcaatac taaataaatc ttggttttgt gcatttaagt
     9181 ttaaccaatg tggagtaact tttcggaaac ttgggaaaaa agtaatagta tttaaaaata
     9241 atttttttat aacttcttct gaagctaaaa tatttttatc aggattatta tcacttttaa
     9301 atgtagttat tttatttcca tacaaattta ataattcctg tgtaaaagat atctctccaa
     9361 ttttttcaca ataatgtatt tcattgttag attgatcatc tataaaccgt aaatatgcag
     9421 aagcactttt actattatta ctggttgata tatcttttcc tgatattact ctaaaatatg
     9481 gattgtcaag attatttgat ttctttacta tatcgttaaa ttgttttttt gctaattcgt
     9541 aaaaagaatc tgctatataa gataaagtat atgttttccc tgagccattt ttccccacta
     9601 aaattattgg tttcggatta aagttttctt gcaattcaaa ttcaaggtct aatttctcaa
     9661 tgggcccaat atttctaatg tgaagatttc ttaagtacat taaatttcca aggttacttt
     9721 aactagttga aaaaaataac ctatcatata attcatcata aaaaactatg tctgttaaag
     9781 attttaaaat gtaaagatca tctaaaacta agataatctg gttatccgaa acagtagcct
     9841 ataactctcg tttttttgtt ctaaaactgc taaaattctt atacgccatt gacgtacttc
     9901 aacctatgtt atttattgaa accagtattt ttaccaagca aattaaagag cttgtgaatg
     9961 atgaagagta tcgtcagctt cagcaggatc tcttggtaca acctgataaa ggtgacttaa
    10021 tcaagaatgg cggtggcatt cgtaaagtac gttgtgctca aggcaataag ggtaaaagtg
    10081 gtggtatccg ggtgatctat tattgggtca cagaagatga tcagatcttt tttctggtgg
    10141 cttatccaaa gtcagtaaaa gataacctga ctgataaaga aacatcgatc ttgcgtcaat
    10201 tagtgaagga gcaatttcat ggataacaac ttatttgatg acttggttgc ttcgattaaa
    10261 gaagctggtg ccatcaaacg caatgaaatg aaagcaagcc gagtgactga gcttgaatta
    10321 ccagatatta aagaagtacg tgagaaaaca ggcttaaccc aagctgaatt tgctgcacgt
    10381 ttgcatatta gcgcgagaac cctacaaaac tgggaacaag gccgtcgcta tccgactggt
    10441 cctgcagcga ctctaattcg cattctggat gcccatccaa ccctaattta aaaagtttag
    10501 ataataaaaa accccggcat cctgccgggg tttttcatgc tttctcatgt gctttcacta
    10561 tcctcggatc cctatcgaac aacttataat tattgtttta tgttatgaaa cttcctgttc
    10621 ccgatgagtt catcatagga aaattccagt atgtcgtata gcagcaaagg gccgagtttc
    10681 atgtgagcca aagcgtacaa aatagcctaa attttatcca caaaaaatgt ggataatttt
    10741 aagcattcaa gcattgataa aatttaaaaa atcgcaaaat tgactatttt ttaaacaatt
    10801 ttattggcaa gaagtatcaa aaccgctcat aaaaaagccg acttgtttca aaatcggctt
    10861 tttgcggttt ttatgatcag gattaatttt ttaagtaatt gatatttaga ttaatataca
    10921 agtgtacttc gtataatgcc cattatgtta aatggggaat aaaatacgat catatattta
    10981 taatccaatt ctttttttga tagaaagaac cattctttct ttatttaaat atctgataca
    11041 gtggcactta tataaaatat tataacttag gtgatccatg cctagcctaa ctctcaaagc
    11101 gacatcaaaa agatgcttcg agcctaaagt tctaatgatt ctaattgagt gtctgcctga
    11161 tgaacaaagg cctcctatta aagagttatt aacctcttat cctgtagaaa ttaaactaaa
    11221 tttaacttca gaagtcttag aacccactgt cgaaaaaata ctaaaacgat taggcagagt
    11281 atttattatc agtaagtaga tcagttctga agttttctta aaattaacat aatacacctt
    11341 atacgaaatg cctgatttac aggcatttct agtgttcagt taacataaaa tctcttatac
    11401 gaatatcaaa caaacagctc aattcatggg tcttctgatc atctgtttaa cataaaactt
    11461 cttatgtgaa gttcgattat aaattctaga tagaaatgtc tacttttaga atacatactt
    11521 ctttgatttt tttattttaa tccagtaaaa atttttaaat tttattggta tagatcttgc
    11581 tgaaaattag atatttactt actaatgcaa ggacttatca attgaataat tttgcaaaat
    11641 cagctttagt tacatcactt ttcacactaa ttacagcctg tggaagctct gacaacgatg
    11701 ataaattagt ttcacagcct tctattgaac caataccatt tgaaccatta gaggccacta
    11761 tctctgattt acatgtttct ttaaaaagta atggatcaaa ttgcgttaat attgtgcaaa
    11821 gttatttaga tcgtatcaat gcatatgata agcaaggtcc tactctaaat agtgtcattt
    11881 cagtaaatcc aaatatatta aaagaagccg ttgaacttga tgagtattat aaaaaaacag
    11941 gtaaattcaa aggagactta cactgtgtca cagtactagc caaagataat atagatgtta
    12001 ttaatatggc aaatacagca ggttcagatg cacttttaga taacgttcca caagatgatg
    12061 cgtatattat taaaaatatt aaagagcaag gtggtttaat tattggcaaa gcaaatcttg
    12121 atgaatttgc ctttggcttt ggtggaaagt ctacagtagg agggcaagcg aagaatgttt
    12181 atgatttaac aaaaggccct ggtggttcat cagcaggtac aggaactgct atttctgcta
    12241 gtttagctag ggtagggatt ggtacggata caggtggcgc gaag
//
