LOCUS       NZ_CP032104             8399 bp    DNA     circular CON 31-MAR-2024
DEFINITION  Acinetobacter lwoffii strain EK30A plasmid pALWEK1.11, complete
            sequence.
ACCESSION   NZ_CP032104
VERSION     NZ_CP032104.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN05251105
            Assembly: GCF_019787625.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter lwoffii
  ORGANISM  Acinetobacter lwoffii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter.
REFERENCE   1  (bases 1 to 8399)
  AUTHORS   Mindlin,S., Petrenko,A., Kurakov,A., Beletsky,A., Mardanov,A. and
            Petrova,M.
  TITLE     Resistance of ancient and modern Acinetobacter lwoffii strains to
            heavy metals and arsenic revealed by genome analysis
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8399)
  AUTHORS   Beletsky,A., Kurakov,A., Petrova,M. and Mardanov,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2018) Lab of molecular cloning, Institute of
            Bioengineering, Research Center of Biotechnology of RAS, Prosp.
            60-let Oktyabrya, bld 7-1, Moscow 117312, Russia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Newbler v. 3.0
            Expected Final Version            :: yes
            Genome Coverage                   :: 25.0x
            Sequencing Technology             :: 454
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_019787625.1-RS_2024_03_31
            Annotation Date                   :: 03/31/2024 06:12:30
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,448
            CDSs (total)                      :: 3,336
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 112
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 222
            CDSs (without protein)            :: 222
            Pseudo Genes (ambiguous residues) :: 0 of 222
            Pseudo Genes (frameshifted)       :: 80 of 222
            Pseudo Genes (incomplete)         :: 138 of 222
            Pseudo Genes (internal stop)      :: 46 of 222
            Pseudo Genes (multiple problems)  :: 39 of 222
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP032104.1.
            Bacteria and source DNA available from Dr. Petrova M.A.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8399
                     /organism="Acinetobacter lwoffii"
                     /mol_type="genomic DNA"
                     /strain="EK30A"
                     /isolation_source="permafrost, Kolyma lowland"
                     /db_xref="taxon:28090"
                     /plasmid="pALWEK1.11"
                     /geo_loc_name="Russia"
     gene            99..365
                     /locus_tag="ABEKA_RS01150"
                     /old_locus_tag="ABWEK_J0001"
     CDS             99..365
                     /locus_tag="ABEKA_RS01150"
                     /old_locus_tag="ABWEK_J0001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005268842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="WP_159373185.1"
                     /translation="MSVTENLDSKIKAQEEKLKQLKAQRQAALARERAKEKEQARKDDT
                     RRKILIGSCMLKITEDDEQARAKLIAQMDKYLTDECDRKLFNL"
                     /name="mobilization protein"
     gene            complement(596..2116)
                     /locus_tag="ABEKA_RS01155"
                     /old_locus_tag="ABWEK_J0002"
     CDS             complement(596..2116)
                     /locus_tag="ABEKA_RS01155"
                     /old_locus_tag="ABWEK_J0002"
                     /EC_number="1.11.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005249302.1"
                     /GO_function="GO:0004096 - catalase activity [Evidence
                     IEA]; GO:0020037 - heme binding [Evidence IEA]; GO:0046872
                     - metal ion binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="WP_159373186.1"
                     /translation="MSQDIKKCPVTHLTTDAGAPVVDNQNSMTAGPRGPLLAQDLWLNE
                     KLANFVREVIPERRMHAKGSGAFGTFTVTNDITPYSRAKIFSEVGKKTEMFARFSTVAG
                     ERGAADAERDIRGFALKFYTEEGNWDMVGNNTPVFFFRDPRQFPDLNKAVKRDPKTNMR
                     SANNNWDFWTLLPEALHQVTVVMSDRGIPASYRHMHGFGSHTYSLINANNERFWVKFHF
                     RTQQGIKNLTDAEAAEVVAKDRESNQNDLYHAIERGEFPKWTLFIQVMPEQDAEKLPYH
                     PFDLTKVWLKADYPLIEVGEFELNRNPENYFQDVEQAAFSPSNVVPGISYSPDRMLQAR
                     LFNYPDAARYRVGVNHSQIPVNAARCPVHSNRRDGQGRIDGNYGSLPHYEPNSFSQWQE
                     QPEYREPPLKISGDADFWDYRQDDSDYFSQPRALFNLMNDEQKQALFDNTARAMGDAYD
                     FIKYRHIRNCYACDPAYGQGVAKSLGLTVADAQNARATDPAFGQPGFL"
                     /name="catalase"
     gene            2383..2880
                     /locus_tag="ABEKA_RS01160"
                     /old_locus_tag="ABWEK_J0003"
     CDS             2383..2880
                     /locus_tag="ABEKA_RS01160"
                     /old_locus_tag="ABWEK_J0003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004783133.1"
                     /GO_function="GO:0008199 - ferric iron binding [Evidence
                     IEA]; GO:0016491 - oxidoreductase activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dps family protein"
                     /protein_id="WP_159373187.1"
                     /translation="MGSVKKQAKLAVIDIGISQGDRAKIVKGLSRLLADSYTLYLMTHN
                     FHWNVTGPQFNSLHNMFMDQYTEQWNALDVIAERIRALGFPAPGTYKQFVELASIQEVE
                     GVPQANEMIQLLVNAHEAVARTARSLFDVVDAANDQPTADVLTQRLDIHEKTAWMLRSL
                     LE"
                     /name="Dps family protein"
     gene            complement(2964..3266)
                     /locus_tag="ABEKA_RS01165"
                     /old_locus_tag="ABWEK_J0004"
     CDS             complement(2964..3266)
                     /locus_tag="ABEKA_RS01165"
                     /old_locus_tag="ABWEK_J0004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_001140620.1"
                     /translation="MAKTLQELLASRSPESQARIQKMADELLLENQLHLIREELEISQK
                     ELAETLGIKQPSLSAIENRGNDLKISTMKKYVEAMGGKLRIDVELPTGKHIGFNV"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(3259..3615)
                     /locus_tag="ABEKA_RS01170"
                     /old_locus_tag="ABWEK_J0005"
     CDS             complement(3259..3615)
                     /locus_tag="ABEKA_RS01170"
                     /old_locus_tag="ABWEK_J0005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005194067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_159373188.1"
                     /translation="MWTVITTDLFNQWFEQQDESTQEKVLAALVVLQQQGPNLGRPLVD
                     AVYESKFTNMKELRVQHRGRPLRAFFAFDPLRQAIVLCIADKGGKKRFYKDMLDIADEQ
                     YRLHLTTLGDKSNG"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            4301..4504
                     /locus_tag="ABEKA_RS01175"
                     /old_locus_tag="ABWEK_J0007"
     CDS             4301..4504
                     /locus_tag="ABEKA_RS01175"
                     /old_locus_tag="ABWEK_J0007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004896916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_057979931.1"
                     /translation="MFNLNIFNRISSEVLTIKNDLELNSENQLITKYKTSTSEDYKKAI
                     ILIFKERGYTRLEIGQLLEGQL"
                     /name="hypothetical protein"
     gene            complement(4690..4977)
                     /locus_tag="ABEKA_RS01180"
                     /old_locus_tag="ABWEK_J0009"
     CDS             complement(4690..4977)
                     /locus_tag="ABEKA_RS01180"
                     /old_locus_tag="ABWEK_J0009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004870774.1"
                     /translation="MRTKAELDAMSHQELKDYEQSLLALWTPRMAIESDIERLSTHHSE
                     LLEVFNQLKNPDAPKNSRLKDSILSLKYKIESLEGKLSDLIQDNRLNSAD"
                     /name="hypothetical protein"
     gene            complement(4974..5915)
                     /locus_tag="ABEKA_RS01185"
                     /old_locus_tag="ABWEK_J0010"
     CDS             complement(4974..5915)
                     /locus_tag="ABEKA_RS01185"
                     /old_locus_tag="ABWEK_J0010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004729701.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_020846814.1"
                     /translation="MDENKKTYPLSWVVMQNNVQECFKSMNIDEKRMLILASPIARTTD
                     ATEKDRIMITAEEFARECGIKTHSAYTQLELASKNLLKRNFSYNNERGKKVLSNWVIDC
                     TYEDGGIAIRFPEIVLLMLKEFDKLNPYTKYKKDIVLSLKKDYSFDLYHLAKKHQAMGQ
                     FEMSLEHIKNELGLPKSYDKLCNLKDRVMKPSLNEITTNTDIELTYENVKKGRSVVGFK
                     FIVKEKPKPKLIAPQRDQRTIDMFCNLSDAQINKYSTILSKLSELSDLSNFPDYPTFAL
                     WISGILRDPKSVREETTKRIFKALHSKTDFKP"
                     /name="replication initiation protein"
     gene            complement(6627..8279)
                     /gene="mobQ"
                     /locus_tag="ABEKA_RS01190"
                     /old_locus_tag="ABWEK_J0012"
     CDS             complement(6627..8279)
                     /gene="mobQ"
                     /locus_tag="ABEKA_RS01190"
                     /old_locus_tag="ABWEK_J0012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005199240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_159373189.1"
                     /translation="MAIYHFSVKTISRGNGRSAVACAAYRSSEKLVCDFYGKEQDYTRK
                     TGVEFTKIYAPENTNTELLKRQTLWNEVEKVERRKDALLAREFEIAFPSELNAEQRENM
                     LNELCRNLVKKYGVIVDAAIHAPHTDSGSDERNYHAHIMFTTRSINEQGGFSAKKYRDF
                     SRDNGTETVSHWRESFAELCNHHLEQNGFDERVDHRSYEDQESDLEATQHEGPQATYLR
                     RRGIFTEISLKNDEIKLRNLKIKKVIALDENIKVSEDLVQKVRSELQFQYSQAEYSEKT
                     SQKLYEFQNEELSKLTTKLDTMSSAISELRKKEPLFFKTKWINQINDLIDQHNTQLDIQ
                     KHISGLSEIEKKERYSDHLNKWTEENQLLQPKKSFAKFDKPNITVKQRKLNDQISNIDH
                     QISEISRRETEYQEYVRDQRLEIINSYIFEEDNYGNKYFSPQNGEKQKRLYAEEMEDLK
                     IQELHAAFTKKIETEAQQEFSQKRAIQLENDRKDRESREQQLRKEREQQEQRYAQEREQ
                     QEHLAFVRRQEQEKQQRNEPKKPENDNNHDYTP"
                     /name="mobQ"
     Typing          complement(6838..8279)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.792"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ctcttcgagg aaaacattac tatacatata gcaccttgaa tgcatattca agtgcgctta
       61 tgttaggcat ggtttcccag gatgatggta ttggatgaat gagcgttact gaaaatttag
      121 acagcaagat taaggcacag gaagagaagc tgaaacagct taaggctcaa agacaggcag
      181 cactggccag agaacgtgcc aaagagaaag aacaggcgcg gaaggatgat accagacgta
      241 agattttaat cggatcctgt atgctaaaaa tcactgaaga tgatgaacag gccagagcta
      301 aactaattgc tcagatggat aagtatttga cggatgagtg tgaccgaaag ctttttaatt
      361 tataaatttt gagagatgag tagtggaaga ttcaacactt aatgagttaa tacctaatat
      421 tctttgaaca tcactggaat gtagatctat taggaaagcc tcctaaatgg tcaccagtac
      481 attttaattt tgggaaaatt actaattacg ataaacaagg tgtatatgcg tttaagagaa
      541 aagtaggagg ataaaatatc ctcctacttt tctcgcttaa aaaattaaat cgctattaca
      601 gaaaaccagg ttgtccaaaa gctggatcag ttgctcgtgc attttgtgca tcagcaacag
      661 tcagacctaa agatttagca acaccttgac cataagctgg atcacaagca taacagttac
      721 gaatatgacg atatttaata aagtcatagg catcgcccat tgcacgtgca gtattgtcaa
      781 ataaagcctg tttctgctca tcattcatta aattaaatag tgcgcgaggc tgactgaagt
      841 agtcgctatc atcttgacgg taatcccaga agtcggcatc accactgatt ttgagtggtg
      901 gttcacgata ttctggttgc tcttgccact ggctgaaact gtttggctcg taatgcggta
      961 agctaccata gtttccatca atacggcctt gaccatcgcg acggttggaa tgtacagggc
     1021 aacgtgcagc attaactgga atttgtgaat ggttaactcc tacacggtaa cgagctgcat
     1081 ctggatagtt aaacaggcga gcttggagca tacggtcagg agagtagctg atgccaggaa
     1141 caacgttgct tggtgagaaa gctgcttgtt ccacatcctg gaaatagttt tctggattgc
     1201 gattcagttc aaattcaccg acttcaatca gtggataatc tgcttttaac cagaccttag
     1261 ttaagtcaaa tggatgataa gggagttttt cagcatcttg ttcaggcatc acttgaatga
     1321 atagcgtcca tttagggaat tcaccacgtt caatcgcatg gtaaagatcg ttctgattgc
     1381 tttcacgatc ttttgcaacg acctctgctg cttctgcatc tgtgagattt ttaatacctt
     1441 gttgagtacg gaaatggaat ttcacccaaa aacgttcgtt attggcatta atcaaactat
     1501 aagtatgact accgaaacca tgcatatggc ggtagcttgc aggaatacca cgatcagaca
     1561 ttactacagt tacttggtgt aatgcttcag gtaacagtgt ccagaaatcc cagttgttgt
     1621 tggcagaacg catgttggtt tttggatcgc gtttaacagc tttgttcagg tcaggaaatt
     1681 gacgtggatc acggaaaaag aagactggag tattattacc caccatgtcc cagttaccct
     1741 cttcagtgta gaatttgagg gcaaaaccac gaatatcacg ttctgcatca gccgcaccac
     1801 gttcaccagc aactgtcgag aaccgagcaa acatttcggt tttcttgcca acttctgaaa
     1861 aaatcttagc gcggctgtat ggtgtgatgt cattcgtaac tgtaaatgta ccaaaagcac
     1921 ctgaaccttt ggcatgcata cgacgctcag gaatgacttc acgaacaaag tttgccagtt
     1981 tttcatttag ccataaatct tgtgcgagta aaggaccccg tggtcctgct gtcatactgt
     2041 tctgattatc tacaacaggt gcccctgcat cagtggtcag atgcgtgact ggacactttt
     2101 taatatcttg gctcatggta tctatccttt cttggtgtaa gaggaaacat cttagctggc
     2161 ttattgtaaa gcagatttat gatgggatta tagaaagatt aaagctaatt tagactgtaa
     2221 taaaaaccaa tcattatata taaatatata atgatatata caaagaaaaa ataattatag
     2281 tcatctaaat agataaaatt aaataattat atttctcttt aaattatgaa cataacaatg
     2341 atatgttcat cctgttgtat tttcctcatc aggagtttaa ttatgggcag cgtaaaaaaa
     2401 caagcgaaat tagcagttat tgatattggt atttcacaag gtgatcgcgc caagattgtc
     2461 aaaggattat cgcgattact cgctgacagt tacacactct atttgatgac tcataatttt
     2521 cactggaacg tcactggccc acagtttaac agcttacata atatgtttat ggatcaatat
     2581 accgagcagt ggaatgcact cgatgtgatt gcagagcgta ttcgtgcctt aggtttccca
     2641 gctccgggta cttacaagca atttgtagaa ttagcttcaa ttcaggaagt ggaaggtgta
     2701 ccccaagcga atgaaatgat tcagttactt gttaatgcac atgaagctgt tgcgcgaact
     2761 gcaagaagtt tatttgatgt tgtagatgca gcgaatgatc agcctacggc agatgttcta
     2821 actcaacgac ttgatattca tgaaaaaact gcatggatgt taagaagcct tttagaataa
     2881 aatataatga aatattgctg attctgagaa aatttacttt tatctcaatc aaaaatggga
     2941 gacttaagct cccattttta atgttaaaca ttgaaaccta tatgttttcc ggttggaagc
     3001 tctacatcaa tacggagctt cccacccatt gcttcaacat actttttcat ggttgaaatt
     3061 ttcagatcat taccacgatt ttctattgcc gaaagcgatg gctgttttat tcccaaagtt
     3121 tcagcaagct ctttttgaga aatttcgagt tcttcacgaa taagatgaag ctgattttct
     3181 agcagtaatt catctgccat tttttgaata cgggcttggc tctctggaga gcggctagct
     3241 aacaattctt gtagagtctt agccattaga tttatctcct agggtggtga gatgaagtcg
     3301 gtattgttca tctgcaatat ctagcatgtc tttataaaaa cgctttttac cgcctttatc
     3361 agcaatacat agaacaatag cctgtcgtaa gggatcaaat gcgaagaaag ctcttagtgg
     3421 tctaccgcga tgttgaacgc gcaattcttt catattggta aatttagatt catacacagc
     3481 atctactaaa ggacggccta aattcggtcc ctgctgctgt agaacaacca gggcagccag
     3541 gaccttctct tgtgtcgatt catcttgctg ttcaaaccac tgattaaata gatctgtcgt
     3601 aatgactgtc cacatactac aaccaaaata tagattatag tctatatttt agggccatta
     3661 tgtttgtttt gcaaatgaga aatgagaggc aataaaaaac cccggtatcc tgccggggtt
     3721 ttttattgtg cgatctttgg taggtgctgc tctacctcac aatcctcgtg ttaaaactta
     3781 tcattgttgt tttatgttcc ggaacatcct gttcctgtat gacttcatca taggaatatt
     3841 ttcgtagtcc ggatagcagc aaagaactgg aattcgagta agccaaagcg tacaaagtgc
     3901 ctgaaattta tccacaagaa atggggataa ttttgaacat tcacgcattg ataaaattta
     3961 aaaaaatcgc aaaattgatt actttttaaa caaatgtatt ggcaagaact atcaaaaccg
     4021 ctcataaaaa agccgacttg tttcaaaatc ggatttttgg gcattcttgg atttttcttg
     4081 ttttattaag tagttgatat ttatattaat atacaagtgt acttcgtata accgccatta
     4141 tgttaagtga acaataattt cgtcgaatat tcaggctctc aattctgcaa aacttgcaga
     4201 atcttgtcta atttgtaatc cttgcggcca agggtgtcta gctaagctaa ggcaggtgtt
     4261 tgttgtagtc gggccgctat aacggatgac gagtgcctgt atgtttaacc taaatatctt
     4321 taataggatc tctagcgaag tattgaccat caaaaatgat ctagagctga attctgagaa
     4381 tcaactgata actaaatata agacttctac ttctgaagat tataaaaaag caattatttt
     4441 gatttttaaa gaacgtggct ataccaggtt agaaataggt cagttacttg aaggccaact
     4501 gtaaagactt cctaaaatta acataataca ccttatacga aatgcgttat aattatctat
     4561 agaaatcaat gctgtagttt ctagttgata gcccatcttc cccaaggtgg gcttttttta
     4621 tgacttttca cgttccacgc ctagtcttta atcaatgttc cacgattttg attaaataaa
     4681 ctgaataatt caatctgcag aatttaagcg attatcttga ataagatcac tcaacttacc
     4741 ttcaagcgat tcgattttat atttcagaga caagattgaa tcttttaaac gtgaattttt
     4801 aggtgcatcc ggattcttta attgattaaa aacctctaat aattcgctgt gatgagtgct
     4861 taacctttca atgtcacttt caatagccat tcttggtgtc cacagagcaa gtaaactttg
     4921 ctcataatct ttgagttctt gatgactcat tgcatctaat tcagccttag ttctcatggt
     4981 ttaaaatccg tcttgctatg aagagcttta aaaatccgtt ttgttgtttc ctctcttaca
     5041 cttttcggat ctcgtaagat gccactaatc cagagggcaa atgtcgggta gtctgggaaa
     5101 ttactgaggt ctgatagctc agaaagttta gataaaatag tgctgtactt gttaatttga
     5161 gcatcagata aattgcagaa catatctata gttctttgat ctcgctgagg cgctattaat
     5221 tttggcttag gtttttcttt cactataaat ttaaagccaa ctactgaacg cccttttttt
     5281 acattttcat aagtgagttc aatatctgta ttagttgtaa tctcatttaa tgaaggcttc
     5341 attactcggt cttttaaatt acagagtttg tcataagatt tgggcaaacc cagctcattt
     5401 tttatatgtt ctaaggacat ttcaaattgt cccattgctt gatgcttttt agctaaatga
     5461 taaagatcga atgagtaatc ttttttcaaa cttagtacga tatctttttt atattttgta
     5521 taagggttta atttatcaaa ctcttttaac atcagtagaa cgatttcagg gaaacgtata
     5581 gctatccctc cgtcttcata agtgcaatca atcacccaat tcgacagcac cttttttcct
     5641 ctttcattgt tataagagaa atttctttta agtagattct tgctggctag ttctaactgc
     5701 gtatatgcgg aatgcgtttt aatgccacat tctctagcaa actcttctgc cgtaatcata
     5761 attcggtctt tttcagttgc atcagtggtt ctagcaattg ggctagcaag tattaacatt
     5821 cgtttttcat caatgttcat actcttaaag cattcttgaa cattgttttg cataacaacc
     5881 cagctcagag gatatgtttt tttattttcg tccatataac tacaacccca aaaaactaac
     5941 taactacatt ggtatttatg tagcttaaac atacattgtc gtttattttt tgactactat
     6001 ttttgtagtt ttttaacaaa actacaaact ttttcttgta gtttaatgtc cggtaaaagt
     6061 agtttctatg tccggtaaaa gtagtttttt aagtccattt tgtccggcat ttgtagttta
     6121 atgtccggta aaagtagttt tttaaagtct gaaagcattg ctaggcgtgg tttatagggg
     6181 ggtctaaaag cttttaaaag ctttttaaaa gaataaaagg gaatttattt ccaaagtgag
     6241 ttattaaaac tgcccttggt actcgctaaa gctcgtactc tattgaagct cgtttcactc
     6301 gcttcgtggg gggcagtttt ttgcatatat gatcaaggat tcaaaaagca aaacatgaat
     6361 aatgcattcg ctatgctcat gctttttgac gagcaaaagc atcaatactt cgctcgtaga
     6421 cactcgctaa tcactgtccc cagtaattca ataaaaaacg gtttttcaga gggggaataa
     6481 gcaaactttc gttgtgtcgc tcgtagatac tcgctaaaag gctctcattt gcgttctaag
     6541 cgattttatt tttatcaact atagttttac tgtatttcta tcaaaagtcg ctaaaacccc
     6601 cgtttttttt cgcttaaatg ggatttctaa ggcgtgtagt catggttatt gtcattttct
     6661 ggctttttag gctcatttct ctgctgtttt tcttgttctt ggcgtcttac aaatgccaaa
     6721 tgttcttgtt gctctcgttc ttgtgcatag cgttgttctt gttgctctcg ttctttacgt
     6781 aactgctgtt ctctagattc acgatctttt cggtcgtttt cgagctgtat tgctcttttt
     6841 tgggaaaact cttgctgcgc ttcggtctca attttttttg taaaagccgc atgcaattct
     6901 tggattttta gatcttccat ttcttctgca tatagtcgct tttgtttttc accattttga
     6961 ggactaaagt atttatttcc atagttatct tcttcaaata tataactatt tatgatctcc
     7021 agtctttgat cgcgtacata ctcttgatac tcagtttctc tgcgtgatat ttcagaaatt
     7081 tgatgatcta tatttgaaat ttgatcatta agttttcgtt gtttaacagt gatgttcggt
     7141 ttgtcaaatt ttgcaaaaga ctttttaggc tggagcagct gattttcttc tgtccattta
     7201 tttaaatgat cagaatatct ctcttttttt tctatttcac taagaccaga aatatgcttt
     7261 tggatgtcta gttgggtgtt gtgttggtcg atcaggtcat ttatttgatt gatccatttt
     7321 gttttaaaaa ataaaggctc ttttttgcgt agttctgata ttgcagaact catcgtgtct
     7381 aattttgttg ttaattttga taattcttca ttttggaatt cataaagttt ctgagaagtt
     7441 ttttctgaat actcagcttg agagtattga aactgaagct cacttcgtac tttttgaaca
     7501 agatcctcag atactttgat gttttcatcc agtgcgatga cctttttgat ttttaaatta
     7561 cgtagcttaa tttcatcatt tttcagacta atttctgtaa atatccctcg tctccggagg
     7621 tatgtcgctt gaggaccttc gtgctgagtt gcttctaggt cactttcctg gtcttcataa
     7681 cttctgtgat cgacacgttc atcaaatcca ttttgttcca ggtgatgatt gcataactct
     7741 gcaaatgatt ctctccagtg gcttacagtt tctgtgccat tgtcacgact gaagtcccta
     7801 tatttttttg ctgaaaagcc accctgctca ttaatacttc gggttgtgaa catgatgtgt
     7861 gcatggtagt tgcgttcatc acttccactg tctgtatgag gtgcatgaat agcagcatca
     7921 acgatgacac cgtatttttt aacgaggttt cggcaaagtt cattgagcat attttcacgc
     7981 tgttcagcgt ttaactcact gggaaatgca atctcaaact ctcttgcaag taatgcatct
     8041 tttctacgtt caaccttttc gacttcattc caaagtgttt ggcgcttgag taactcagta
     8101 tttgtatttt caggtgcata gatttttgta aattcgacac cagtttttct tgtgtagtct
     8161 tgttcttttc cataaaaatc gcataccaat ttttcacttg aacgatatgc agcacaagca
     8221 acggccgatc gtccattacc tctactaatc gttttaactg aaaaatgata tatcgccatt
     8281 ttgcttttgc tctttatttc gatttatcga aattaccaac atgtatatgt tggtcagggg
     8341 attacaaagg ggatttaccc cttgtcgcac cattggatga aatccaatgg ctaagtgcg
//
