LOCUS       NZ_CP032103             9238 bp    DNA     circular CON 31-MAR-2024
DEFINITION  Acinetobacter lwoffii strain EK30A plasmid pALWEK1.10, complete
            sequence.
ACCESSION   NZ_CP032103
VERSION     NZ_CP032103.2
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN05251105
            Assembly: GCF_019787625.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter lwoffii
  ORGANISM  Acinetobacter lwoffii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter.
REFERENCE   1  (bases 1 to 9238)
  AUTHORS   Rakitin,A.L., Ermakova,A.Y., Beletsky,A.V., Petrova,M.,
            Mardanov,A.V. and Ravin,N.V.
  TITLE     Genome analysis of Acinetobacter lwoffii strains isolated from
            permafrost soils aged from 15 thousand to 1.8 million years revealed
            their close relationships with present-day clinical isolates
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9238)
  AUTHORS   Rakitin,A.L., Ermakova,A.Y., Beletsky,A.V., Petrova,M.,
            Mardanov,A.V. and Ravin,N.V.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2021) Institute of Bioengineering, Research Center
            of Biotechnology of the Russian Academy of Sciences, Moscow 119071,
            Russia
  REMARK    Nucleotide sequence updated by submitter
REFERENCE   3  (bases 1 to 9238)
  AUTHORS   Beletsky,A., Kurakov,A., Petrova,M. and Mardanov,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2018) Lab of molecular cloning, Institute of
            Bioengineering, Research Center of Biotechnology of RAS, Prosp.
            60-let Oktyabrya, bld 7-1, Moscow 117312, Russia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Newbler v. 3.0
            Expected Final Version            :: yes
            Genome Coverage                   :: 25.0x
            Sequencing Technology             :: 454
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_019787625.1-RS_2024_03_31
            Annotation Date                   :: 03/31/2024 06:12:30
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,448
            CDSs (total)                      :: 3,336
            Genes (coding)                    :: 3,114
            CDSs (with protein)               :: 3,114
            Genes (RNA)                       :: 112
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 222
            CDSs (without protein)            :: 222
            Pseudo Genes (ambiguous residues) :: 0 of 222
            Pseudo Genes (frameshifted)       :: 80 of 222
            Pseudo Genes (incomplete)         :: 138 of 222
            Pseudo Genes (internal stop)      :: 46 of 222
            Pseudo Genes (multiple problems)  :: 39 of 222
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP032103.2.
            On Aug 29, 2021 this sequence version replaced NZ_CP032103.1.
            Bacteria and source DNA available from Dr. Petrova M.A.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9238
                     /organism="Acinetobacter lwoffii"
                     /mol_type="genomic DNA"
                     /strain="EK30A"
                     /isolation_source="permafrost, Kolyma lowland"
                     /db_xref="taxon:28090"
                     /plasmid="pALWEK1.10"
                     /geo_loc_name="Russia"
     gene            join(8735..9238,1..1185)
                     /gene="mobQ"
                     /locus_tag="ABEKA_RS01105"
                     /old_locus_tag="ABEKA_3301"
     CDS             join(8735..9238,1..1185)
                     /gene="mobQ"
                     /locus_tag="ABEKA_RS01105"
                     /old_locus_tag="ABEKA_3301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005199240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_222110375.1"
                     /translation="MAIYHFSVKTISRGNGRSAVACAAYRSSEKLVCDFYGKEQDYTRK
                     TGVEFTKIYAPENTNTELLKRQTLWNEVEKVERRKDALLAREFEIAFPSELNAEQRENM
                     LNELCRNLVKKYGVIVDAAIHAPHTDSGSDERNYHAHIMFTTRSINEQGGFSAKKYRDF
                     SRDFSAKKYRDFSRDNGTETVSHWRESFAELCNHHLEQNGFDERVDHRSYEDQESDLEA
                     TQHEGPQATYLRRRGIFTEISLKNDEIKLRNLKIKKVIALDENIKVSEDLVQKVRSELQ
                     FQYSQAEYSEKTSQKLYEFQNEELSKLTTKLDTMSSAISELRKKEPLFFKTKWINQIND
                     LIDQHNTQLDIQKHISGLSEIEKKERYSDHLNKWTEENQLLQPKKSFAKLDEPNITVKQ
                     RKLNDQISNIDHQISEISRRETEYQEYVRDQRLEIINSYIFEEDNYGNKYFSPQNGEKQ
                     KRLYAEEMEDLKIQELHAAFTKKIETEAQQEFSQKIAIQLENDRKDRESREQQLRKERE
                     QQEQRYAQEREQQEHLAFLRRQEQEKQQRNEPKKPENDNNHDYTP"
                     /name="mobQ"
     gene            1868..2818
                     /locus_tag="ABEKA_RS01110"
                     /old_locus_tag="ABEKA_3293"
     CDS             1868..2818
                     /locus_tag="ABEKA_RS01110"
                     /old_locus_tag="ABEKA_3293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001031297.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_004728629.1"
                     /translation="MNKENSYDKSYPVTTMAIQNKVTECFKSMSVDEKRILIMASPIAR
                     NIDASEQDQILISAQQFADDCGIKVNSAYKQIENASKKLVDRSFSYVNDRGKKVYSNWV
                     IDATYEDAGISLRFTSIVLVMLKILDKYNPYTRYKKDVVLKLKKDYSIDFYHLAKKNQA
                     KNGFELTLDEMFTEFGLPESYRDLRNLKRRVLKSSLDEINEFTDVTVDYSPVKKGRSVV
                     GFKFTVKEKSKPKLIAPERDPKTIDIFCNLSDAQINKYSAILSKLSELSDLSNFQDYPS
                     FALWISGILRDPKSVREETAKRIFKALHSKTDFKP"
                     /name="replication initiation protein"
     gene            2815..3102
                     /locus_tag="ABEKA_RS01115"
                     /old_locus_tag="ABEKA_3294"
     CDS             2815..3102
                     /locus_tag="ABEKA_RS01115"
                     /old_locus_tag="ABEKA_3294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004728627.1"
                     /translation="MKTRAELDAMSHQELKDYEQSLLALWTPRMAIESDIERLSTNRNE
                     LLEIFNQLKNPDAPENERLKNSILSLKYKIEDLEDKLDDLIQDNRLNRAD"
                     /name="hypothetical protein"
     gene            complement(3330..4181)
                     /locus_tag="ABEKA_RS01120"
                     /old_locus_tag="ABEKA_3295"
     CDS             complement(3330..4181)
                     /locus_tag="ABEKA_RS01120"
                     /old_locus_tag="ABEKA_3295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="WP_002115246.1"
                     /translation="MSKIIACIDGSLATNTVCDYAAWFSDKLNSPLQLLHVIDKPKAKA
                     PQDLSGAIGLGSRETLLNELVELEERKGKIELEHGQILLREAKNYLLEKFSIDAQSFQR
                     HGSLLETIMGMEEDIRVLVMGKHGTETEHNSSKVGTHIENVVRALHKPVLITSSPFSPP
                     QSFLIAFDGSPTARICVDKIANSPLLKTLTAHVVYVGKPNSDMTSQVTWAKELLANNGF
                     DVIDSVLEGDVEKSILDYQEKNVIDMIVIGAYGHSKIRQFFIGSTTTKLLSSSTKPVLL
                     LR"
                     /name="universal stress protein"
     gene            complement(4194..5681)
                     /locus_tag="ABEKA_RS01125"
                     /old_locus_tag="ABEKA_3296"
     CDS             complement(4194..5681)
                     /locus_tag="ABEKA_RS01125"
                     /old_locus_tag="ABEKA_3296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000953322.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="WP_005146104.1"
                     /translation="MLSTIREQWFSNIRGDVLAGIVVALALIPEAIAFSIIAGVDPKVG
                     LYASFCIAVVISFVGGRPGMISAATGAMALLMVTLVKEHGLQYLLAATILTGFIQILAG
                     YLKLGALMRFVSKSVVIGFVNALAILIFMAQLPELTNVTWHVYAMTIGGLAIIYLFPYI
                     PVIGKLLPSPLICIVLLTLFALFIGLDVRTVGDMGQLPDTLPIFLLPDIPLNLETLAII
                     LPYSLGLAAVGLLESMMTATIVDDLTDTTSDKNRECKGQGVANVASGFLGGMAGCAMIG
                     QSIINVKSGGRTRLSTFSAGVFLLIMVVFISDWLKVIPMAALVAVMIMVSIGTFNWDSL
                     RNIRQYPFSSNIVMIATVVVVVATHNLAYGVLVGVLLSALFFANKIERYMAIQSEFNEP
                     ESTRTYTVTGQVFFSSADKFTSAFDFKEALSKVVIDVSQAHFWDVSAVAAVDKVVIKFR
                     REGTEVELIGMNEASKTLVDKFGIHDKPDALDRLNSH"
                     /name="SulP family inorganic anion transporter"
     gene            complement(6109..6606)
                     /locus_tag="ABEKA_RS01130"
                     /old_locus_tag="ABEKA_3297"
     CDS             complement(6109..6606)
                     /locus_tag="ABEKA_RS01130"
                     /old_locus_tag="ABEKA_3297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="WP_018680090.1"
                     /translation="MKIELTDKERLFLANQHEILGHLNTDNSDYHFKLAEQLRDGHEWL
                     YSQSFDYFSENLPSEDADLVLDILQVYELIGDTYSRLSDKSGISDTQVKFPGFDGNNEP
                     EFMSFVDALEKDNRYVDVIQAGNRNSHSAKVHVYERMIAKWQELGKPYTLTKEQLIDIL
                     EA"
                     /name="YfbU family protein"
     gene            complement(6693..6938)
                     /locus_tag="ABEKA_RS01135"
                     /old_locus_tag="ABEKA_3298"
     CDS             complement(6693..6938)
                     /locus_tag="ABEKA_RS01135"
                     /old_locus_tag="ABEKA_3298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012540186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_018680089.1"
                     /translation="MKHPEIKPYDWIRVGNRNCVVMNIYPSNSPFGVCKVVFNPQKPTT
                     HDVDWNGQQWFFPERPDFGGYGRDGCPFVRKLKQGI"
                     /name="hypothetical protein"
     gene            7147..8136
                     /locus_tag="ABEKA_RS01140"
                     /old_locus_tag="ABEKA_3299"
     CDS             7147..8136
                     /locus_tag="ABEKA_RS01140"
                     /old_locus_tag="ABEKA_3299"
                     /inference="COORDINATES: protein motif:HMM:NF019371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="WP_018680088.1"
                     /translation="MLIPYNKSYLTLDQQIEKLKVNGLIIHNENYAKKCLISYNYYRLS
                     AYWHLFRQRDPNDSTKKLGKFESGHTFEEVIQIYEFDYQLRKLIFEAITDIETFLRTQV
                     AYHIPGINNDAFAMYDQSLFYMRSRRNLKGIDYQGLMKGINTEISRAKGEDFISHFKNT
                     YNDTYDDHPKLPFWVLTEIVSFGTLRKIYKHLKSSHKHLISDSFNIGTTSSSSPSPYIH
                     IDKENLNSHLFLINKYRNVCAHHGRLWNRKVKDVLTADAATELQISDHTNGGLYALIIV
                     IARLLEPTGHAKDWKDSIYCLLEKNFPLGDITKFTTLPLDWHKHPIWK"
                     /name="Abi family protein"
     gene            complement(8221..8523)
                     /locus_tag="ABEKA_RS01145"
                     /old_locus_tag="ABEKA_3300"
     CDS             complement(8221..8523)
                     /locus_tag="ABEKA_RS01145"
                     /old_locus_tag="ABEKA_3300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_018680087.1"
                     /translation="MSVETLEQKIAKQEERLRQLKAQKQAIAAREKKKVSDQQRKDDTR
                     RKILLGAWALNKLKNDESFKQQLADFEQFLNTENKTEENKQKDKDLFNGFIWDKT"
                     /name="hypothetical protein"
     Typing          1869..2818
                     /name="rep_cluster_1171"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.79"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          2..1182
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.97"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          8736..9232
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.47e-106"
ORIGIN
        1 gcaaaaaaat atagggactt cagtcgtgac aatggcacag aaactgtaag ccactggaga
       61 gaatcatttg cagagttatg caatcatcac ctggaacaaa atggatttga tgaacgtgtc
      121 gatcacagaa gttatgaaga ccaggaaagt gacctagaag caactcagca cgaaggtcct
      181 caagcgacat acctccggag acgagggata tttacagaaa ttagtctgaa aaatgatgaa
      241 attaagctac gtaatttaaa aatcaaaaag gtcatcgcac tggatgaaaa catcaaagta
      301 tctgaggatc ttgttcaaaa agtacgaagt gagcttcagt ttcaatactc tcaagctgag
      361 tattcagaaa aaacttctca gaaactttat gaattccaaa atgaagaatt atcaaaatta
      421 acaacaaaat tagacacgat gagttctgca atatcagaac tacgcaaaaa agagccttta
      481 ttttttaaaa caaaatggat caatcaaata aatgacctga tcgaccaaca caacacccaa
      541 ctagacatcc aaaagcatat ttctggtctt agtgaaatag aaaaaaaaga gagatattct
      601 gatcatttaa ataaatggac agaagaaaat cagctgctcc agcctaaaaa gtcttttgca
      661 aaacttgacg aaccgaacat cactgttaaa caacgaaaac ttaatgatca aatttcaaat
      721 atagatcatc aaatttctga aatatcacgc agagaaactg agtatcaaga gtatgtacgc
      781 gatcaaagac tggagatcat aaatagttat atatttgaag aagataacta tggaaataaa
      841 tactttagtc ctcaaaatgg tgaaaaacaa aagcgactat atgcagaaga aatggaagat
      901 ctaaaaatcc aagaattgca tgcggctttt acaaaaaaaa ttgagaccga agcgcagcaa
      961 gagttttccc aaaaaatagc aatacagctc gaaaacgacc gaaaagatcg tgaatctaga
     1021 gaacagcagt tacgtaaaga acgagagcaa caagaacaac gctatgcaca agaacgagag
     1081 caacaagaac atttggcatt tttaagacgc caagaacaag aaaaacagca gagaaatgag
     1141 cctaaaaagc cagaaaatga caataaccat gactacacgc cttagcaatc ccatttaagc
     1201 gcaaaaaaac gggggtttta gcgacttttg atagaaatac agtaaaacta tagttgataa
     1261 aaataaaatc gcttagaacg caaatgagag ccttttagcg agtgtctacg agcgacacaa
     1321 cgaaaggttg cttattcccc ctctgaaaaa ccgtttttta ttgaattact ggggacagtg
     1381 attagcgagt gtctacgagc gaagtattga tgcttttgct cgtcaaaaag catgagcata
     1441 gcgaatgcat tattcatgtt ttgctttttg aatccttgat catatatgca aaaaactgcc
     1501 cccacgaagc gagtgaaacg agcttcaata gagtacgagc tttagcgagt accaagggca
     1561 gttttaataa ctcactttgg aaataaattc ccttttattc ttttaaaaag cttttaaaag
     1621 cttttagacc ctctgaaaac cttatgcagc aatagtttca agcgttaaaa gggtacaaat
     1681 agcatgataa agggtacaaa tagcatgatt ttatcgttta aaagggtaca aatagcatga
     1741 ttaaagggta caaatagcat gataaagggt acaaataaat tattgtaccc ttatgtttat
     1801 tggtgtacat tgttttctca taagggtaca aaaatcatgt tcatttttta taaggtttga
     1861 tatctgaatg aataaagaaa atagttatga taaatcttat ccagtaacaa caatggctat
     1921 tcaaaacaaa gttactgaat gctttaaaag catgtctgta gatgaaaaaa gaattttaat
     1981 tatggcttct ccgattgcta gaaatatcga tgcaagtgaa caagatcaaa tcttaatatc
     2041 tgctcaacaa tttgctgatg actgtggtat caaagtcaat tctgcttata aacaaattga
     2101 aaatgcgtca aaaaaactag tagatcggtc cttttcatac gttaatgata gggggaaaaa
     2161 ggtctactct aactgggtaa ttgatgctac ttatgaagat gcagggatat ctttaaggtt
     2221 tacatctatt gttttggtga tgttgaaaat tttagataaa tacaatccat acactcgtta
     2281 taaaaaagat gtagttctaa aattaaaaaa agactactca atagactttt accatttagc
     2341 gaaaaaaaat caggcaaaaa atggctttga attaacgcta gatgaaatgt ttacagagtt
     2401 tggtttacca gaatcttata gagatttgag gaacttaaaa cgaagagttt tgaagagctc
     2461 attagatgaa attaatgaat ttaccgatgt aacagttgac tatagtccag ttaaaaaagg
     2521 acgttctgtt gtcggcttta agttcactgt gaaagaaaaa tctaagccaa aattaatagc
     2581 tcctgagcga gatccaaaaa caatagatat attctgcaac ctgtctgatg ctcaaattaa
     2641 caagtacagt gctattttat ctaaactttc tgagctatca gacctaagta acttccagga
     2701 ctatcctagt tttgcccttt ggattagtgg catcttacga gatccgaaaa gtgtaagaga
     2761 ggaaacagca aagcggattt ttaaagctct tcatagcaag acggatttta aaccatgaaa
     2821 actagagctg aattagatgc aatgagccat caagaactca aagactatga gcaaagttta
     2881 cttgctctgt ggacaccaag aatggctatt gaaagtgaca ttgaaaggtt aagcactaat
     2941 cgcaacgaat tattagaaat ttttaatcaa ttaaaaaatc ctgatgctcc tgaaaatgag
     3001 cgtttaaaaa attctatttt atcgctcaaa tataagattg aagatttaga agataaattg
     3061 gatgatctta ttcaagataa tcgcttaaat cgtgcagatt gaattattca gtttatttaa
     3121 ttaaaaccgt gaaacattga tcaaagaata ggcgtggaac gtgaatagtc acaaaaaagc
     3181 cgttccagtg cgctgaaccc tggaacgtct tagtataagt ttatgattta tatcgaataa
     3241 aaaacagtat ttcgtataag gtgtattatg ttaattttag aaattctaaa aaagggatag
     3301 tttttaacta tccctttatc ttgtaaaaat tatcttagta ataatactgg ttttgtagaa
     3361 cttgataaca atttggtcgt cgtactacca ataaaaaact ggcgtatctt tgaatgacca
     3421 tatgcaccaa tcacaatcat atcaataacg tttttttctt gataatccaa tatagatttc
     3481 tcaacatcac cttcaagcac actatcaatg acatcaaaac cattatttgc aagtagctct
     3541 tttgcccaag tgacttgtga tgtcatatca ctatttggct taccaacata taccacatga
     3601 gctgtcaggg tttttaataa agggctattt gcaattttgt ctacacaaat acgtgcagta
     3661 ggacttccat caaaagcaat taaaaatgac tgaggtggac taaacggtga ggatgtgatt
     3721 aacacgggtt tatgaagagc acgaacaaca ttttcaatat gggttccaac tttactggaa
     3781 ttgtgctcgg tttcagttcc atgcttaccc ataacaagaa ctcgaatatc ctcttccatt
     3841 cccatgattg tttctaaaag actgccatgg cgttgaaagc tttgagcatc aatagagaac
     3901 ttctccaata gatagttttt tgcttcccga agaagaattt ggccatgctc tagctcaatt
     3961 ttccccttac gttcttccag ttcaaccaac tcgtttagca aggtttctcg acttcctaga
     4021 ccaattgcac cacttaaatc ttgtggtgct ttcgcttttg gcttatcaat gacatgcaat
     4081 aactgaagag gtgaattcaa tttatcgcta aaccaagctg cataatcaca aacggtattt
     4141 gttgctaatg atccatcaat acatgcaata atttttgaca taattcatcc ctcttagtgg
     4201 ctatttaagc gatctaaagc atctggctta tcatggatac caaacttatc gactaacgtt
     4261 ttactggctt cattcattcc aatcagttcg acttctgttc cttcacgtct aaacttaatg
     4321 accactttat ctacggcagc gaccgcagac acatcccaaa agtgagcctg actaacatca
     4381 atcacaactt ttgaaagtgc ttctttgaaa tcaaatgcag acgtaaattt atctgccgag
     4441 ctgaagaaca cttgacccgt caccgtataa gtacgggtac tttcaggttc attaaattcg
     4501 gactgaattg ccatgtagcg ttcaattttg ttagcaaaaa ataatgcaga aagaagaaca
     4561 ccgactaata cgccataagc caggttatgt gtggcaacaa ccacaaccac tgtcgctatc
     4621 attacgatat tgctagagaa gggatattgg cgaatattgc gcagtgaatc ccagttaaat
     4681 gttccgattg aaaccatgat cattacggca accagtgcag ccattggaat gactttgagc
     4741 cagtcactga taaacaccac cataattaac aggaagaccc ccgcactaaa ggtagataaa
     4801 cgggtacgac caccagattt cacattaata attgattgac cgatcatggc acagcctgcc
     4861 attccgccta agaagcctga agcgacattg gcaacgcctt gtcctttaca ctcacggttt
     4921 ttatcactgg tggtatctgt taaatcgtcg acaatcgttg ctgtcatcat agattcaagc
     4981 aaaccgacgg ctgctaatcc cagtgaataa ggcaaaataa tagctaaagt ttcaagattt
     5041 aaaggaatat caggaagcaa gaaaattgga agagtatctg gtaattgccc catatcacct
     5101 acggttctta catctaaacc aatgaacaag gcaaagagcg tgaggagtac aatacagatg
     5161 aggggagagg gtaaaagctt gcctattact gggatatagg ggaatagata aataatggct
     5221 aaaccaccaa ttgtcatcgc atagacatgc catgtcacat tggtgagttc aggcaattgg
     5281 gccataaaaa ttagaatagc caaggcattc acaaagccaa tcacaaccga ctttgacaca
     5341 aagcgcatca atgcacctaa ttttagatag cctgctagga tttgaataaa tcctgtcaaa
     5401 attgtggctg ctaataagta ttgcagacca tgctctttaa ccagtgtgac cattaataaa
     5461 gccatagcac cagttgcagc agaaatcatc cctggacgac cgcctacgaa agaaatcacc
     5521 acggcaatac agaatgaagc atataaccct accttagggt cgacacctgc aataattgaa
     5581 aaggcaattg cttcaggaat tagagccaaa gcaacaacaa tacccgcaag aacatccccg
     5641 cgaatattgg agaaccattg ttctcgaata gtggataaca tgaaaatagc ctattttttt
     5701 aaattttaag aaaggctagt gatgtgatat atacgacaca caagctacta caccacatca
     5761 ctagacagtg atgagttatt tactgattta agaggtaaat aaacttatgg tggcgtaagt
     5821 gtcatattaa gaaagatatc tattttggag gatttcgggg gcaatcatag cagctagccc
     5881 cttttctatc caccctaaac gcagtactga agtaaaagct aagaattatc atagataatt
     5941 ttagaattga tttatgccca tttaacataa tggcggttat acgaaataaa aaaaaaacct
     6001 aggcattagc ctaggtctta aattttaaaa aacacataaa aattaaggct atagtctcaa
     6061 ttttcaactt cacataacgt gtattctatt aattttaaga actcttaatt atgcctctaa
     6121 aatatcgatt agctgttcct tagttaaagt ataaggctta ccaagctctt gccatttagc
     6181 aatcattcgt tcatatacat gaactttagc tgaatgagag tttctgttgc ctgcttggat
     6241 cacatcaaca tagcgattat ctttttccag agcatcaaca aaactcataa actcaggctc
     6301 attattccca tcaaaaccag ggaactttac ttgagtatct gaaataccac ttttatcaga
     6361 taatcgactg taagtatcac caattagttc atagacctgt aggatatcta aaacaaggtc
     6421 agcatcttcc gatggtaagt tttcagagaa gtaatcaaaa ctttgagagt atagccactc
     6481 atgaccatct cgtagctgtt cagctaactt aaagtgataa tccgaattat ccgtatttaa
     6541 atgacctaaa atttcatgct gattagctag aaataaacgc tctttatctg ttaattcaat
     6601 tttcatttgt tcatcttata aagtattgga actctatata tctatttatt attttatttt
     6661 tcaaggtatt atccctatta atttcactta atttatattc cttgttttag ctttcgaaca
     6721 aatggacatc catctctacc atatcctcca aaatcaggac gttcagggaa aaaccattgt
     6781 tgaccattcc aatcaacgtc atgagtagta ggtttttgtg gattgaatac aactttacat
     6841 actccaaaag gagagttact aggataaata ttcataacaa cgcagttacg attacctacc
     6901 cttatccaat catatggttt aatttctgga tgtttcatgg cactttaccc cttattagat
     6961 acttactttt aaaaataatc ttttaaatat taaatatata tgaaaaaatg ctttactatt
     7021 tagtatatgc tatctagtat attaattaga taccgcttcg cctctgtcaa cattcttgac
     7081 atatatatat cgagttatcg atatattgcc tactaagcgg ttttttttcg tctggagaaa
     7141 agttccttgt tgatcccata caacaaaagc tatctaacat tagatcaaca aattgaaaaa
     7201 cttaaagtca atggactgat cattcataat gaaaattacg ctaaaaaatg tttaatcagt
     7261 tacaactatt atcgacttag tgcttattgg catttattca gacaacgaga tccaaatgat
     7321 agtactaaaa agttaggtaa attcgaatca ggtcatactt ttgaagaagt tatacaaatt
     7381 tatgagttcg actatcaatt acgtaaacta atttttgaag caattactga tatagaaaca
     7441 tttctaagaa ctcaggtcgc ctaccatata ccgggtatca acaatgatgc attcgctatg
     7501 tatgatcaat cactgttcta catgcgatca cgtagaaatt tgaaaggtat cgactatcag
     7561 gggttgatga aaggtatcaa cactgaaata tcccgtgcta aaggcgaaga ctttattagt
     7621 cactttaaga atacttataa tgatacttat gatgaccacc caaaactacc attttgggtg
     7681 ctaactgaaa ttgtgagttt cggcacttta agaaaaattt acaaacacct taaatcatca
     7741 cataagcatt taataagtga tagtttcaat attggtacga catcatcaag ttcaccatct
     7801 ccatacattc atatagataa ggaaaactta aacagtcatt tattcttaat taataaatat
     7861 cgtaatgttt gtgctcatca tggcaggctt tggaatagaa aagttaagga tgtacttact
     7921 gctgatgctg ctacagaact acaaatatct gaccatacaa atggtgggct gtatgcccta
     7981 attattgtca ttgctagatt actagagcct acaggtcacg ctaaagattg gaaagattca
     8041 atatattgtc ttttagaaaa aaactttccg ttaggagata tcacaaagtt tacaacactc
     8101 cccttggact ggcataagca tccaatttgg aaataaccaa accccgataa atatcagttt
     8161 ttttattaat atgctttaca ttttttgaga ttatttaaca taaaatctct tatgcgaaat
     8221 ttaggtctta tcccaaataa atccattaaa aagatcttta tctttctgct tattttcttc
     8281 ggtcttattt tccgtattta aaaattgctc aaaatcggct aattgctgtt taaacgattc
     8341 atcatttttt aatttattta aagcccaagc tcccagtaga attttacgtc tcgtatcatc
     8401 tttacgttgc tgatcagaaa ctttcttttt ttcacgagca gcaatagctt gtttttgtgc
     8461 ttttagttgt cttaatcgtt cttcttgctt tgcaattttt tgctctaaag tttcaacact
     8521 cataccatca ccatctcaag gaatccccct caagcatagg tctgtttgaa ttgaaattca
     8581 agacagccca tgctatcgtg gttttcccga agggcgcact tagccattgg atttcatcca
     8641 atggtgcgac aaggggtaaa tcccctttgt aatcccctga ccaacatata catgttggta
     8701 atttcgataa atcgaaataa agagcaaaag caaaatggcg atatatcatt tttcagttaa
     8761 aacgattagt agaggtaatg gacgatcggc cgttgcttgt gctgcatatc gttcaagtga
     8821 aaaattggta tgcgattttt atggaaaaga acaagactac acaagaaaaa ctggtgtcga
     8881 atttacaaaa atctatgcac ctgaaaatac aaatactgag ttactcaagc gccaaacact
     8941 ttggaatgaa gtcgaaaagg ttgaacgtag aaaagatgca ttacttgcaa gagagtttga
     9001 gattgcattt cccagtgagt taaacgctga acagcgtgaa aatatgctca atgaactttg
     9061 ccgaaacctc gttaaaaaat acggtgtcat cgttgatgct gctattcatg cacctcatac
     9121 agacagtgga agtgatgaac gcaactacca tgcacacatc atgttcacaa cccgaagtat
     9181 taatgagcag ggtggctttt cagcaaaaaa atatagggac ttcagtcgtg acttttca
//
