LOCUS       NZ_CP032082             6647 bp    DNA     circular CON 19-JUN-2023
DEFINITION  Escherichia coli strain CT30 plasmid p.CT30-P4, complete sequence.
ACCESSION   NZ_CP032082
VERSION     NZ_CP032082.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN09939627
            Assembly: GCF_018141265.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6647)
  AUTHORS   Zhuge,X.
  TITLE     Molecular Epidemiology and Zoonotic Risk of Avian-origin
            mcr-1-positive Extraintestinal Pathogenic Escherichia coli in China
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6647)
  AUTHORS   Zhuge,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2018) College of Veterinary Medicine, Nanjing
            Agricultural University, No.1, Weigang Road, Xuanwu District,
            Nanjing, Jiangsu 210095, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: ABySS v. 2.0.2
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 506.0x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/19/2023 03:03:00
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,271
            CDSs (total)                      :: 5,152
            Genes (coding)                    :: 4,904
            CDSs (with protein)               :: 4,904
            Genes (RNA)                       :: 119
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 248
            CDSs (without protein)            :: 248
            Pseudo Genes (ambiguous residues) :: 0 of 248
            Pseudo Genes (frameshifted)       :: 90 of 248
            Pseudo Genes (incomplete)         :: 169 of 248
            Pseudo Genes (internal stop)      :: 49 of 248
            Pseudo Genes (multiple problems)  :: 56 of 248
            Pseudo Genes (short protein)      :: 4 of 248
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP032082.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6647
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="CT30"
                     /host="Avian"
                     /db_xref="taxon:562"
                     /plasmid="p.CT30-P4"
                     /geo_loc_name="China"
                     /collection_date="2014"
     gene            4..162
                     /locus_tag="D2195_RS26485"
     CDS             4..162
                     /locus_tag="D2195_RS26485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071531517.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHALTPLAAATGNRISRAMMA
                     QTVLQSS"
                     /name="replication initiation protein"
     gene            660..887
                     /locus_tag="D2195_RS25970"
                     /old_locus_tag="D2195_28125"
     CDS             660..887
                     /locus_tag="D2195_RS25970"
                     /old_locus_tag="D2195_28125"
                     /inference="COORDINATES: protein motif:HMM:NF015489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E1 immunity protein"
                     /protein_id="WP_230498631.1"
                     /translation="MLYAIVISTILCPYSKYAIEHIAFNFIKKDFFERRKNLNNAPVAK
                     LNLFMLYNLLCLVLAIPFGLLGLFISIKNN"
                     /name="colicin E1 immunity protein"
     gene            complement(884..2452)
                     /gene="cea"
                     /locus_tag="D2195_RS25975"
                     /old_locus_tag="D2195_28130"
     CDS             complement(884..2452)
                     /gene="cea"
                     /locus_tag="D2195_RS25975"
                     /old_locus_tag="D2195_28130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin E1"
                     /protein_id="WP_000447006.1"
                     /translation="METAVAYYKDGVPYDDKGQVIITLLNGTPDGSGSGGGGGKGGSKS
                     ESSAAIHATAKWSTAQLKKTQAEQAARAKAAAEAQAKAKANRDALTQRLKDIVNEALRH
                     NASRTPSATELAHANNAAMQAEAERLRLAKAEEKARKEAEAAEKAFQEAEQRRKEIERE
                     KAETERQLKLAEAEEKRLAALSEEAKAVEIAQKKLSAAQSEVVKMDGEIKTLNSRLSSS
                     IHARDAEMKTLAGKRNELAQASAKYKELDELVKKLSPRANDPLQNRPFFEATRRRVGAG
                     KIREEKQKQVTASETRINRINADITQIQKAISQVSNNRNAGIARVHEAEENLKKAQNNL
                     LNSQIKDAVDATVSFYQTLTEKYGEKYSKMAQELADKSKGKKIGNVNEALAAFEKYKDV
                     LNKKFSKADRDAIFNALASVKYDDWAKHLDQFAKYLKITGHVSFGYDVVSDILKIKDTG
                     DWKPLFLTLEKKAADAGVSYVVALLFSLLAGTTLGIWGIAIVTGILCSYIDKNKLNTIN
                     EVLGI"
                     /name="cea"
     gene            2712..3149
                     /locus_tag="D2195_RS25980"
                     /old_locus_tag="D2195_28135"
     CDS             2712..3149
                     /locus_tag="D2195_RS25980"
                     /old_locus_tag="D2195_28135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001405929.1"
                     /translation="MGHCHFMAWHTLRDALKMTGRSRSQFYRDMRAGLVSYRTGRDGRR
                     EFETSELIRAYGELKQNETPERHSEGHAENPHDQQTERILRELNELKQCLTLMLEDKQA
                     QDMDRRRQEAEREQLQNEIAQLRQALELEKKRGFWSRLFGR"
                     /name="hypothetical protein"
     gene            3230..3649
                     /locus_tag="D2195_RS25985"
                     /old_locus_tag="D2195_28140"
     CDS             3230..3649
                     /locus_tag="D2195_RS25985"
                     /old_locus_tag="D2195_28140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_001254967.1"
                     /translation="MRRTGNKLCLIAMITATVALTACTPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLRGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            complement(3967..4200)
                     /locus_tag="D2195_RS25990"
     CDS             complement(3967..4200)
                     /locus_tag="D2195_RS25990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137445.1"
                     /translation="MTELETHLLNALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSEQTERLSQLYSENWR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(4207..4725)
                     /locus_tag="D2195_RS25995"
     CDS             complement(4207..4725)
                     /locus_tag="D2195_RS25995"
                     /inference="COORDINATES: protein motif:HMM:NF016710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_000067500.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNNEFRRLGESVSEAVTSNE
                     TKIRDAIALFTASTEESLEKHREGVKEAMMQHRRDVLKLAGNTGMMLLGIVFLLFTASG
                     GTLWYLGGRIQANLEEIRKQEETLQKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVIPFQ
                     GKEWVHLKE"
                     /name="MbeB family mobilization protein"
     gene            complement(<4763..5194)
                     /locus_tag="D2195_RS26490"
                     /pseudo=""
     CDS             complement(<4763..5194)
                     /locus_tag="D2195_RS26490"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(5403..5726)
                     /locus_tag="D2195_RS26005"
                     /old_locus_tag="D2195_28160"
     CDS             complement(5403..5726)
                     /locus_tag="D2195_RS26005"
                     /old_locus_tag="D2195_28160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000955999.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            5790..5981
                     /locus_tag="D2195_RS26010"
                     /old_locus_tag="D2195_28165"
     CDS             5790..5981
                     /locus_tag="D2195_RS26010"
                     /old_locus_tag="D2195_28165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165985.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          complement(6067..6154)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.86e-39"
     Typing          complement(2..93)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="97.849"
                     /coverage="99.0"
                     /evalue="2.5000000000000003e-37"
     Typing          6165..6647
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.639"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(3864..5413)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.473"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
       61 gagtccaacc cggtaaggca cgccttaacg ccactggcag cagccactgg taaccggatt
      121 agcagagcga tgatggcaca aacggtgcta cagagttctt gaagtagtgg cccgactacg
      181 gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
      241 aaagagttgg tagctcttga tccggcaaac aaaccaccgt tggtagcggt ggtttttttg
      301 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
      361 ctactgaacc gcgatccccg tcagtttaga agaggaggat ggtgcgatgg tccctccctg
      421 aacatcacgt atatagttag cctgacatcc aacaaggagg tttatcgcga atattcccac
      481 aaaaaatctt ttcctcataa ctcgatcctt ataaaatgaa aagaatatat ggcgaggttt
      541 aatttatgag cttaagatac tacataaaaa atattttatt tggcctgtac tgcacactta
      601 tatatatata ccttataaca aaaaacagcg aggggtatta tttccttgtg tcagataaga
      661 tgctatatgc aatagtgata agcactattc tatgtccata ttcaaaatat gctattgaac
      721 acatagcttt taacttcata aagaaagatt ttttcgaaag aagaaaaaac ctaaataacg
      781 cccccgtagc aaaattaaac ctatttatgc tatataatct actttgtttg gtcctagcaa
      841 tcccatttgg attgctagga ctttttatat caataaagaa taattaaatc cctaacacct
      901 catttatagt attaagttta ttcttatcaa tataggagca tagaattcct gtaacaatag
      961 caatacccca aatacctaat gtagttccag caagcaagct aaaaagtaaa gcaacaacat
     1021 aactcacccc tgcatctgca gctttcttct ctaatgtaag aaatagtggc ttccagtcac
     1081 ctgtatcctt aatttttagg atatcagata ccacatcata tccaaaagaa acatgccccg
     1141 taatcttcaa gtacttggca aactgatcta aatgtttagc ccagtcatca tacttcaccg
     1201 atgccaacgc attaaaaata gcatcacgat cggctttgct gaatttctta tttaaaacat
     1261 ccttgtattt ttcaaaagca gcgagagctt cattcacatt gccgattttc ttacctttag
     1321 acttatcagc aagttcctgt gccattttcg aatatttttc accatatttt tcagtcagcg
     1381 tttgataaaa gctaactgtt gcatcaacag catccttaat ctgtgaatta aggagattat
     1441 tctgtgcttt tttcaaattt tcttcagctt catgaacacg agcgataccg gcattacgat
     1501 tattactgac ctgagaaata gccttctgga tctgagttat atcagcattt atccggttaa
     1561 tacgtgtttc tgatgctgtt acctgttttt gtttttcttc tctaatctta ccggccccaa
     1621 cccgtcgtct ggttgcttca aaaaaaggac ggttctgaag cggatcattg gctcttggtg
     1681 atagtttttt gaccagctca tccagttctt tatatttagc ggatgcctga gccagttcat
     1741 ttcgttttcc agcgagcgtt ttcatttctg catcacgggc atggatactg gagcttaaac
     1801 gagaattgag agtcttaatc tctccatcca ttttcaccac ttcagattgt gcagcagaaa
     1861 gttttttttg ggcgatctca acagctttag cttcttcact caatgcagcc agtcgtttct
     1921 cttcagcttc agccagtttc aactggcgtt ctgtttcagc cttctcccgt tcaatctctt
     1981 tacgtcgttg ttctgcttcc tgaaaagcct tttctgctgc ttccgcttct ttacgggctt
     2041 tttcttctgc tttcgcaagg cgcaaacgct ctgcttccgc ctgcatagct gcattattag
     2101 catgagcaag ctctgttgct gaaggcgtac gtgaggcatt gtgacgaaga gcctcattca
     2161 cgatatcctt caggcgctga gtcagcgcat ccctgtttgc ctttgctttc gcctgtgctt
     2221 ccgctgcagc ttttgcccgg gcagcctgct ctgcctgtgt tttctttaat tgagcagtag
     2281 accatttagc agttgcatga atagctgcag aactttcact tttactgcct ccttttccac
     2341 ctccgccgcc agagccactc ccgtcaggag taccattcaa aagagtaata attacctgtc
     2401 ccttatcatc ataaggaaca ccatctttat agtacgctac cgcggtttcc attataaaat
     2461 cctctttgac ttttaaaaca ataagttaaa aataaatact gtacatataa ccactggttt
     2521 tatatacagc ataaaagcta cgccgctgca ttttccctgt caagactgtg gacttccatt
     2581 tttgtgaaaa cgatcaaaaa aacagtcttt cacaccacgc gctattctcg cccgatgcca
     2641 caaaaaccag cacaaacatt accgttctca gacctcatta tgttttactg aaactatgag
     2701 atgagacatc tatgggacac tgtcacttta tggcatggca cacactccgg gacgcactaa
     2761 aaatgacagg cagatcgcgt tcacagtttt accgtgatat gcgcgcaggc cttgtcagtt
     2821 accgtaccgg cagggacgga cgacgggagt ttgaaaccag tgaactgatc cgggcatacg
     2881 gcgaattaaa gcagaatgag acaccagaaa ggcacagtga gggacatgca gaaaatccac
     2941 atgatcagca gacagaacgc attctccggg agctgaatga gctgaaacaa tgcctgacgc
     3001 tgatgcttga ggataaacag gcacaggata tggatcgcag acgccaggaa gcagaacggg
     3061 aacagctaca aaatgagata gcccagctca ggcaggcact ggaactggaa aagaaacggg
     3121 gattctggtc caggttgttc ggtcgctgaa cgctgtcaga gactgatgat aaaatagtct
     3181 tcggataata actcaccgag aataaatact ttaaggtagg gagacactca tgagacgtac
     3241 cggaaacaaa ctttgtctta tcgccatgat aacagcaaca gtagctctca cagcctgtac
     3301 cccaaagggc agcgtggaac aacatacccg gcattacgta tatgcttctg atgacggttt
     3361 tgatcccaac ttttccaccc aaaaagccga cacaacacga atgatggtgc ctttttttcg
     3421 gcagttctgg gatatgggag ctaaagataa agcgacagga aaatcacgga gtgatgtgca
     3481 acaacgcatt cagcagtttc acagccagga atttttaaac tcactccggg gcacaactca
     3541 atttgcgggt actgattacc gcagcaaaga ccttaccccg aaaaaatcca ggctgctggc
     3601 tgacacgatt tctgcggttt atctcgatgg ctacgagggc agacagtaag tggatttacc
     3661 ataatccctt aattgtacgc accgctaaaa cgtgttcagc gcgatcacgg cagcagacag
     3721 gtaaaaatgg caacaaacca cccgaaaaac tgccgcgatc gcgcctgata aattttaacc
     3781 gtatgaatac ctatgcaacc agagggtaca ggccacatta cccccactta atccactgaa
     3841 gctgccattt ttcatggttt caccatccca gcgaagggcc atccagcgtg cgttcctgta
     3901 tttccggctg acgctcccgt tccagggata acacatgttc gcgctcctgt atcagccgtt
     3961 cctctcttat ctccagttct cgctgtataa ctggctcaag cgttctgtct gctcgctcaa
     4021 gtgctgcacc tgctgactca actgcatgac ccgctcgttc agcatcgcgt tgtcccgttg
     4081 cgtaagcgaa aacatcttct gcaattccac gaaggcgctc tcccattcgc tcagccgctg
     4141 catatagtcc tgttgcagct gctctaaggc gttcagcaaa tgtgtttcca gctctgtcac
     4201 tctgtgtcac tccttcagat gtacccactc tttcccctga aagggaatca cctccgctga
     4261 tttcccgtac ggaaggacaa ggaatttcct gttcccgtcc tgcacaaact ccacgcccca
     4321 tgtcttcgcg ttcagtttct gcaatgtctc ttcctgcttc ctgatttctt ccaggttcgc
     4381 ctgtatcctc cctccaagat accagagcgt cccgccactc gcggtaaaca ggagaaagac
     4441 tatccccagt aacatcatgc ccgtattccc tgccagcttt aacacgtccc tcctgtgctg
     4501 catcatcgcc tctttcaccc cttcccggtg tttttccagc gattcctctg tcgaggctgt
     4561 gaacagggct atagcgtctc tgattttcgt ctcgtttgat gtcacagcct cgcttacaga
     4621 ttcgccgagc ctcctgaact cgttgttcag cattttctct gtagattcgg ctctctcttt
     4681 cagctttttc tcgaactccg cgcccgtctg caaaagattg ctcataaaat gctcctttca
     4741 gcctgatatt cttcccgccg ttcggatctg caatgctgat actgcttcgc gtcaccctga
     4801 ccacttccag ccccgcctca gtgagcgcct gaatcacatc ctgacggcct tttatctctc
     4861 cggcatggta aagtgcatct atacctcgcg tgacgccctc agcaagcgcc tgtttcgttt
     4921 caggcaggtt atcagggagt gtcagcgtcc tgcggttctc cggggcgttc gggtcatgca
     4981 gcccgtaatg gtgattcacc agcgtctgcc aggcatcaat tctaggcctg tctgcgcggt
     5041 cgtagtacgg ctggaggcgt tttccggtct gtagctccat gttcggaatg acaaaattca
     5101 gctcaagccg tcccttgtcc tggtgctcca cccacaggat gctgtactga tttttttcga
     5161 gaccgggcat cagtacacgc tcaaagctcg ccatcacttt ttcacgtcct cccggcggca
     5221 gctccttctc cgcgaacgac agaacaccgg acgtgtattt cttcgcaaat ggcgtggcat
     5281 cgatgagttc ccggacttct tccggattac cctgaagcac cgttgcgcct tcgcggttac
     5341 gctccctccc cagcaggtaa tcaaccggac cactgccacc accttttccc ctggcatgaa
     5401 atttaactat catcccgcgc cccctgttcc ctgacggcca gacgcagccg gcgcagctca
     5461 tccccgatgg ccatcagtgc ggccaccacc tgaacccggt caccggaaga ccactgcccg
     5521 ctgttcacct tacgggctgt ctgattcagg ttatttccga tggcggccag ctgacgcagt
     5581 aacggcggtg ccagtgtcgg cagttttccg gaacgggcaa ccggctcccc caggcagacc
     5641 cgccgcatcc ataccgccag ttgtttaccc tcacagcgtt caagtaaccg ggcatgttca
     5701 tcatcagtaa cccgtattgt gagcatcctc tcgcgtttca tcggtatcat taccccatga
     5761 acagaaatcc cccttacacg gaggcatcag tgactaaaca ggaaaaaacc gcccttaaca
     5821 tggcccgctt tatcagaagc cagacattaa cgctgctgga gaagctcaac gaactggacg
     5881 cagatgaaca ggccgatatt tgtgaatcgc ttcacgacca cgccgatgag ctttaccgca
     5941 gctgcctcgc acgtttcggg gatgacggtg aaaacctctg acacatgcag ctcccggaga
     6001 cggtcacagc ttgtctgtga gcggatgccg ggagctgaca agcccgtcag ggcgcgtcag
     6061 caggttttag cgggtgtcgg ggcgcagccc tgacccagtc acgtagcgat agcggagtgt
     6121 atactggctt aaccatgcgg catcagtgcg gattgtatga aaagtacgcc atgccgggtg
     6181 tgaaatgccg cacagatgcg taaggagaaa atgcacgtcc aggcgctttt ccgcttcctc
     6241 gctcactgac tcgctacgct cggtcgttcg actgcggcga gcggtactga ctcacacaaa
     6301 aacggtaaca cagttatcca cagaatcagg ggataaggcc ggaaagaaca tgtgagcaaa
     6361 agaccagaaa caggaagaag gccgcgtagc aggcgttttt ccataggctc cgcccccctg
     6421 acgagcatca caaaaataga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     6481 gacaccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     6541 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     6601 gctgttggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggc
//
