LOCUS       NZ_CP032077             6635 bp    DNA     circular CON 19-JUN-2023
DEFINITION  Escherichia coli strain CT29 plasmid p.CT29-P4, complete sequence.
ACCESSION   NZ_CP032077
VERSION     NZ_CP032077.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN09939625
            Assembly: GCF_018141245.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6635)
  AUTHORS   Zhuge,X.
  TITLE     Molecular Epidemiology and Zoonotic Risk of Avian-origin
            mcr-1-positive Extraintestinal Pathogenic Escherichia coli in China
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6635)
  AUTHORS   Zhuge,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2018) College of Veterinary Medicine, Nanjing
            Agricultural University, No.1, Weigang Road, Xuanwu District,
            Nanjing, Jiangsu 210095, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: ABySS v. 2.0.2
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 503.0x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/19/2023 03:02:27
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,761
            CDSs (total)                      :: 4,636
            Genes (coding)                    :: 4,409
            CDSs (with protein)               :: 4,409
            Genes (RNA)                       :: 125
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 14
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 57 of 227
            Pseudo Genes (incomplete)         :: 147 of 227
            Pseudo Genes (internal stop)      :: 57 of 227
            Pseudo Genes (multiple problems)  :: 31 of 227
            Pseudo Genes (short protein)      :: 3 of 227
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP032077.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6635
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="CT29"
                     /host="Avian"
                     /db_xref="taxon:562"
                     /plasmid="p.CT29-P4"
                     /geo_loc_name="China"
                     /collection_date="2014"
     gene            complement(join(6506..6635,1..983))
                     /gene="intI1"
                     /locus_tag="D2194_RS23465"
                     /old_locus_tag="D2194_25400"
     CDS             complement(join(6506..6635,1..983))
                     /gene="intI1"
                     /locus_tag="D2194_RS23465"
                     /old_locus_tag="D2194_25400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="WP_024166810.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAFI
                     RFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQEI
                     GRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDFDHG
                     TIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALERKYPR
                     AGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATPHTLRH
                     SFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLTSGHCCK
                     VSDEAAFCLIQRPYISKTLLTRRISPRGSP"
                     /name="intI1"
     gene            1156..1608
                     /locus_tag="D2194_RS23470"
                     /old_locus_tag="D2194_25405"
     CDS             1156..1608
                     /locus_tag="D2194_RS23470"
                     /old_locus_tag="D2194_25405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000237816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)--rifampin ADP-ribosyltransferase Arr-2"
                     /protein_id="WP_000237816.1"
                     /translation="MVKDWIPISHDNYKQVQGPFYHGTKANLAIGDLLTTGFISHFEDG
                     RILKHIYFSALMEPAVWGAELAMSLSGLEGRGYIYIVEPTGPFEDDPNLTNKKFPGNPT
                     QSYRTCEPLRIVGVVEDWEGHPVELIRGMLDSLEDLKRRGLHVIED"
                     /name="NAD(+)--rifampin ADP-ribosyltransferase Arr-2"
     gene            1929..3188
                     /locus_tag="D2194_RS23475"
                     /old_locus_tag="D2194_25410"
     CDS             1929..3188
                     /locus_tag="D2194_RS23475"
                     /old_locus_tag="D2194_25410"
                     /function="4 : Transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001256773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloramphenicol efflux MFS transporter CmlA5"
                     /protein_id="WP_012300772.1"
                     /translation="MRSKNFSWRYSLAATVLLLSPFDLLASLGMDMYLPAVPFMPNALG
                     TTASTIQLTLTTYLVMIGAGQLLFGPLSDRLGRRPVLLGGGLAYVVASMGLALTSSAEV
                     FLGLRILQACGASACLVSTFATVRDIYAGREESNVIYGILGSMLAMVPAVGPLLGALVD
                     MWLGWRAIFAFLGLGMIAASAAAWRFWPETRVQRVAGLQWSQLLLPVKCLNFWLYTLCY
                     AAGMGSFFVFFSIAPGLMMGRQGVSQLGFSLLFATVAIAMVFTARFMGRVIPKWGSPSV
                     LRMGMGCLIAGAVLLAITEIWASQSVLGFIAPMWLVGIGVATAVSVSPNGALRGFDHVA
                     GTVTAVYFCLGGVLLGSIGTLIISLLPRNTAWPVVVYCLTLATVVLGLSCVSRVKGSRG
                     QGEHDVVALQSAESTSNPNR"
                     /name="chloramphenicol efflux MFS transporter CmlA5"
     gene            3453..4253
                     /locus_tag="D2194_RS23480"
                     /old_locus_tag="D2194_25415"
     CDS             3453..4253
                     /locus_tag="D2194_RS23480"
                     /old_locus_tag="D2194_25415"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032488483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxacillin-hydrolyzing class D beta-lactamase
                     OXA-10"
                     /protein_id="WP_000846390.1"
                     /translation="MKTFAAYVIIACLSSTALAGSITENTSWNKEFSAEAVNGVFVLCK
                     SSSKSCATNDLARASKEYLPASTFKIPNAIIGLETGVIKNEHQVFKWDGKPRAMKQWER
                     DLTLRGAIQVSAVPVFQQIAREVGEVRMQKYLKKFSYGNQNISGGIDKFWLEGQLRISA
                     VNQVEFLESLYLNKLSASKENQLIVKEALVTEAAPEYLVHSKTGFSGVGTESNPGVAWW
                     VGWVEKETEVYFFAFNMDIDNESKLPLRKSIPTKIMESEGIIGG"
                     /name="oxacillin-hydrolyzing class D beta-lactamase OXA-10"
     gene            4270..5108
                     /gene="aadA1"
                     /locus_tag="D2194_RS23485"
                     /old_locus_tag="D2194_25420"
                     /pseudo=""
     ARG             4271..5058
                     /name="aadA1"
                     /gene="aadA1"
                     /gene_name="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            5136..5609
                     /gene="dfrA14"
                     /locus_tag="D2194_RS23490"
                     /old_locus_tag="D2194_25425"
     ARG             5137..5606
                     /name="dfrA14"
                     /gene="dfrA14"
                     /gene_name="trimethoprim-resistant dihydrofolate reductase
                     DfrA14"
                     /tool="amrfinderplus"
                     /drug_class="TRIMETHOPRIM"
                     /identity="100.0"
                     /coverage="100.0"
     gene            5856..6560
                     /locus_tag="D2194_RS23495"
                     /old_locus_tag="D2194_25430"
     CDS             5856..6560
                     /locus_tag="D2194_RS23495"
                     /old_locus_tag="D2194_25430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="WP_001067855.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDHS
                     TIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTVDF
                     YLSSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCPSDVEHR
                     QIKYRNNVIECDHGKLKRIIGATLGFKSMKTAYATIKGIEVMRALRKGQASAFYYGDPL
                     GEMRLVSRVFEM"
                     /name="IS6-like element IS26 family transposase"
     ARG             1157..1605
                     /name="arr-2"
                     /gene="arr-2"
                     /gene_name="NAD(+)--rifampin ADP-ribosyltransferase Arr-2"
                     /tool="amrfinderplus"
                     /drug_class="RIFAMYCIN"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             3454..4250
                     /name="blaOXA-10"
                     /gene="blaOXA-10"
                     /gene_name="oxacillin-hydrolyzing class D beta-lactamase
                     OXA-10"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             1930..3185
                     /name="cmlA5"
                     /gene="cmlA5"
                     /gene_name="chloramphenicol efflux MFS transporter CmlA5"
                     /tool="amrfinderplus"
                     /drug_class="PHENICOL"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             4247..5061
                     /name="aadA"
                     /gene="aadA"
                     /gene_name="ANT(3'')"
                     /tool="rgi"
                     /drug_class="aminoglycoside antibiotic"
                     /identity="99.62"
                     /coverage="103.04"
ORIGIN
        1 tcaagcggtg agcgcactcc ggcaccgcca actttcagca catgcgtgta aatcatcgtc
       61 gtagagacgt cggaatggcc gagcagatcc tgcacggttc gaatgtcgta accgctgcgg
      121 agcaaggccg tcgcgaacga gtggcggagg gtgtgcggtg tggcgggctt cgtgatgcct
      181 gcttgttcta cggcacgttt gaaggcgcgc tgaaaggtct ggtcatacat gtgatggcga
      241 cgcacgacac cgctccgtgg atcggtcgaa tgcgtgtgct gcgcaaaaac ccagaaccac
      301 ggccaggaat gcccggcgcg cggatacttc cgctcaaggg cgtcgggaag cgcaacgccg
      361 ctgcggccct cggcctggtc cttcagccac catgcccgtg cacgcgacag ctgctcgcgc
      421 aggctgggtg ccaagctctc gggtaacatc aaggcccgat ccttggagcc cttgccctcc
      481 cgcacgatga tcgtgccgtg atcgaaatcc agatccttga cccgcagttg caaaccctca
      541 ctgatccgca tgcccgttcc atacagaagc tgggcgaaca aacgatgctc gccttccaga
      601 aaaccgagga tgcgaaccac ttcatccggg gtcagcacca ccggcaagcg ccgcgacggc
      661 cgaggtcttc cgatctcctg aagccagggc agatccgtgc acagcacctt gccgtagaag
      721 aacagcaagg ccgccaatgc ctgacgatgc gtggagaccg aaaccttgcg ctcgttcgcc
      781 agccaggaca gaaatgcctc gacttcgctg ctgcccaagg ttgccgggtg acgcacaccg
      841 tggaaacgga tgaaggcacg aacccagtgg acataagcct gttcggttcg taagctgtaa
      901 tgcaagtagc gtatgcgctc acgcaactgg tccagaacct tgaccgaacg cagcggtggt
      961 aacggcgcag tggcggtttt catggcttgt tatgactgtt tttttgtaca gtctatgcct
     1021 cgggcatcca agcagcaagc gcgttacgcc gtgggtcgat gtttgatgtt atggagcagc
     1081 aacgatgtta cgcagcaggg cagtcgccct aaaacaaagt tatgcagcca aatcccaaca
     1141 attaagggtc ttaaaatggt aaaagattgg attcccatct ctcatgataa ttacaagcag
     1201 gtgcaaggac cgttctatca tggaaccaaa gccaatttgg cgattggtga cttgctaacc
     1261 acagggttca tctctcattt cgaggacggt cgtattctta agcacatcta cttttcagcc
     1321 ttgatggagc cagcagtttg gggagctgaa cttgctatgt cactgtctgg cctcgagggt
     1381 cgcggctaca tatacatagt tgagccaaca ggaccgttcg aagacgatcc gaatcttacg
     1441 aacaaaaaat ttcccggtaa tccaacacag tcctatagaa cctgcgaacc cttgagaatt
     1501 gttggcgttg ttgaagactg ggaggggcat cctgttgaat taataagggg aatgttggat
     1561 tcgttagagg acttaaagcg ccgtggttta cacgtcattg aagactagtc ctttgcataa
     1621 caaagccatc aaaccggacg ccagagattc cgcgcctgtt gcgcatggct tcgccatttt
     1681 atgcgcaata ggcgcgccac cctgtcgccg tttatggcgg cgttgggcgc acaataaggc
     1741 tcctcgcaga gttgcttgaa agttgttacg attcaaattc aatcatgaga tagtcagcag
     1801 atgagcactt ccaagaacgc agacaagtaa gccgcagcaa ccttcatttt tcggttgttg
     1861 cggcgttctc atgaatcctt ttgctctacg ggagcgccgc caaatccttt gttcaaggag
     1921 atggtttcgt gcgctcaaaa aactttagtt ggcggtactc ccttgccgcc acggtgttgt
     1981 tgttatcacc gttcgattta ttggcatcac tcggcatgga catgtacttg ccagcagtgc
     2041 cgtttatgcc aaacgcgctt ggtacgacag cgagcacaat tcagcttacg ctgacaacgt
     2101 acttggtcat gattggtgcc ggtcagctct tgtttggacc gctatcggac cgactggggc
     2161 gccgccccgt tctactggga ggtggcctcg cctacgttgt ggcgtcaatg ggcctcgctc
     2221 ttacgtcatc ggctgaagtc tttctggggc ttcggattct tcaggcttgt ggtgcctcgg
     2281 cgtgccttgt ttccacattt gcaacagtac gtgacattta cgcaggtcgc gaggaaagta
     2341 atgtcattta cggcatactc ggatccatgc tggccatggt cccggcggta ggcccattgc
     2401 tcggagcgct cgtcgacatg tggcttgggt ggcgggctat ctttgcgttt ctaggtttgg
     2461 gcatgatcgc tgcatctgca gcagcgtggc gattctggcc tgaaacccgg gtgcaacgag
     2521 ttgcgggctt gcaatggtcg cagctgctac tccccgttaa gtgcctgaac ttctggttgt
     2581 acacgttgtg ttacgccgct ggaatgggta gcttcttcgt ctttttctcc attgcgcccg
     2641 gactaatgat gggcaggcaa ggtgtgtctc agcttggctt cagcctgctg ttcgccacag
     2701 tggcaattgc catggtgttt acggctcgtt ttatggggcg tgtgataccc aagtggggca
     2761 gcccaagtgt cttgcgaatg ggaatgggat gcctgatagc tggagcagta ttgcttgcca
     2821 tcaccgaaat atgggcttcg cagtccgtgt taggctttat tgctccaatg tggctagtgg
     2881 gtattggtgt cgccacagcg gtatctgtgt cgcccaatgg cgctcttcga ggattcgacc
     2941 atgttgctgg aacggtcacg gcagtctact tctgcttggg cggtgtactg ctaggaagca
     3001 tcggaacgtt gatcatttcg ctgttgccgc gcaacacggc ttggccggtt gtcgtgtact
     3061 gtttgaccct tgcaacagtc gtgctcggtc tgtcttgtgt ttcccgagtg aagggctctc
     3121 gcggccaggg ggagcatgat gtggtcgcgc tacaaagtgc ggaaagtaca tcaaatccca
     3181 atcgttgaga gaatgtggca agctatcgcc caacaaatcg ctgcagccga cccaaaaccg
     3241 ctacgcggtt tcggtcggct gagctcaggc gttaggcctc gccgaagcga aagatcgcta
     3301 cttgaagtgt tgacgccttt gttttaaagt ttcgcgtcta tctttctgat tttgttaaaa
     3361 aatcagactt tgattaatca tgttagacgt cagaggtatt ttgacttaaa cgcctctgag
     3421 gtcgaattaa cgttagccac caagaaggtg ccatgaaaac atttgccgca tatgtaatta
     3481 tcgcgtgtct ttcgagtacg gcattagctg gttcaattac agaaaatacg tcttggaaca
     3541 aagagttctc tgccgaagcc gtcaatggtg tcttcgtgct ttgtaaaagt agcagtaaat
     3601 cctgcgctac caatgactta gctcgtgcat caaaggaata tcttccagca tcaacattta
     3661 agatccccaa cgcaattatc ggcctagaaa ctggtgtcat aaagaatgag catcaggttt
     3721 tcaaatggga cggaaagcca agagccatga agcaatggga aagagacttg accttaagag
     3781 gggcaataca agtttcagct gttcccgtat ttcaacaaat cgccagagaa gttggcgaag
     3841 taagaatgca gaaatacctt aaaaaatttt cctatggcaa ccagaatatc agtggtggca
     3901 ttgacaaatt ctggttggaa ggccagctta gaatttccgc agttaatcaa gtggagtttc
     3961 tagagtctct atatttaaat aaattgtcag catctaaaga aaaccagcta atagtaaaag
     4021 aggctttggt aacggaggcg gcacctgaat atctagtgca ttcaaaaact ggtttttctg
     4081 gtgtgggaac tgagtcaaat cctggtgtcg catggtgggt tgggtgggtt gagaaggaga
     4141 cagaggttta ctttttcgcc tttaacatgg atatagacaa cgaaagtaag ttgccgctaa
     4201 gaaaatccat tcccaccaaa atcatggaaa gtgagggcat cattggtggc taaaacaaag
     4261 ttaaacatca tgagggaagt ggtgatcgcc gaagtatcga ctcaactatc agaggtagtt
     4321 ggcgtcatcg agcgccatct cgaaccgacg ttgctggccg tacatttgta cggctccgca
     4381 gtggatggcg gcctgaagcc acacagtgat attgatttgc tggttacggt gaccgtaagg
     4441 cttgatgaaa caacgcggcg agctttgatc aacgaccttt tggaaacttc ggcttcccct
     4501 ggagagagcg agattctccg cgctgtagaa gtcaccattg ttgtgcacga cgacatcatt
     4561 ccgtggcgtt atccagctaa gcgcgaactg caatttggag aatggcagcg caatgacatt
     4621 cttgcaggta tcttcgagcc agccacgatc gacattgatc tggctatctt gctgacaaaa
     4681 gcaagagaac atagcgttgc cttggtaggt ccagcggcgg aggaactctt tgatccggtt
     4741 cctgaacagg atctatttga ggcgctaaat gaaaccttaa cgctatggaa ctcgccgccc
     4801 gactgggctg gcgatgagcg aaatgtagtg cttacgttgt cccgcatttg gtacagcgca
     4861 gtaaccggca aaatcgcgcc gaaggatgtc gctgccgact gggcaatgga gcgcctgccg
     4921 gcccagtatc agcccgtcat acttgaagct agacaggctt atcttggaca agaagaagat
     4981 cgcttggcct cgcgcgcaga tcagttggaa gaatttgttc actacgtgaa aggcgagatc
     5041 accaaggtag tcggcaaata atgtctaaca attcgttcaa gccgacgccg cttcgcggcg
     5101 cggcttaact caagcgttaa cccaggatga gaaccttgaa agtatcattg atggctgcga
     5161 aagcgaaaaa cggcgtgatt ggttgcggtc cagacatacc ctggtccgcg aaaggggagc
     5221 agctactttt taaagcattg acctacaatc agtggcttct ggtgggtcgc aagacgtttg
     5281 aatctatggg cgcactcccc aataggaaat acgcggtcgt tacccgctca ggttggacat
     5341 caaatgatga caatgtagtt gtatttcagt caatcgaaga ggccatggac aggctagctg
     5401 aattcaccgg tcacgttata gtgtctggtg gcggagaaat ttaccgagaa acattaccca
     5461 tggcctctac gctccactta tcgacgatcg acatcgagcc agagggggat gttttcttcc
     5521 cgagtattcc aaataccttc gaagttgttt ttgagcaaca ctttacttca aacattaact
     5581 attgctatca aatttggaaa aagggttaac aaagctatgc aatcgacggc aaaaagcttc
     5641 gttcgcttcg cgcactacgc cttttccgcg attgatagcg acgttatgtg aatattgaaa
     5701 tgagcattcc aaagaaagga agtagaaaaa ttatcgtggg cgaaaatgag tttttgtggc
     5761 tcattaggtc aaaactcaca tattcgcagg acggcactgt tgcaaatagt cggtggtgat
     5821 aaacttatca tccccttttg ctgatggagc tgcacatgaa cccattcaaa ggccggcatt
     5881 ttcagcgtga catcattctg tgggccgtac gctggtactg caaatacggc atcagttacc
     5941 gtgagctgca ggagatgctg gctgaacgcg gagtgaatgt cgatcactcc acgatttacc
     6001 gctgggttca gcgttatgcg cctgaaatgg aaaaacggct gcgctggtac tggcgtaacc
     6061 cttccgatct ttgcccgtgg cacatggatg aaacctacgt gaaggtcaat ggccgctggg
     6121 cgtatctgta ccgggccgtc gacagccggg gccgcactgt cgatttttat ctctcctccc
     6181 gtcgtaacag caaagctgca taccggtttc tgggtaaaat cctcaacaac gtgaagaagt
     6241 ggcagatccc gcgattcatc aacacggata aagcgcccgc ctatggtcgc gcgcttgctc
     6301 tgctcaaacg cgaaggccgg tgcccgtctg acgttgaaca ccgacagatt aagtaccgga
     6361 acaacgtgat tgaatgcgat catggcaaac tgaaacggat aatcggcgcc acgctgggat
     6421 ttaaatccat gaagacggct tacgccacca tcaaaggtat tgaggtgatg cgtgcactac
     6481 gcaaaggcca ggcctcagca ttttattatg gtgatcccct gggcgaaatg cgcctggtaa
     6541 gcagagtttt tgaaatgtaa ggcctttgaa taagacaaaa ggctgcctca tcgctaactt
     6601 tgcaacagtg cccactagtg aggggcggca gcgca
//
