LOCUS       NZ_CP032071             4110 bp    DNA     circular CON 19-JUN-2023
DEFINITION  Escherichia coli strain E166 plasmid p.E166-P5, complete sequence.
ACCESSION   NZ_CP032071
VERSION     NZ_CP032071.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN09939624
            Assembly: GCF_018141225.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4110)
  AUTHORS   Zhuge,X.
  TITLE     Molecular Epidemiology and Zoonotic Risk of Avian-origin
            mcr-1-positive Extraintestinal Pathogenic Escherichia coli in China
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4110)
  AUTHORS   Zhuge,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2018) College of Veterinary Medicine, Nanjing
            Agricultural University, No.1, Weigang Road, Xuanwu District,
            Nanjing, Jiangsu 210095, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: ABySS v. 2.0.2
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 606.0x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/19/2023 03:03:17
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,177
            CDSs (total)                      :: 5,061
            Genes (coding)                    :: 4,819
            CDSs (with protein)               :: 4,819
            Genes (RNA)                       :: 116
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 242
            CDSs (without protein)            :: 242
            Pseudo Genes (ambiguous residues) :: 0 of 242
            Pseudo Genes (frameshifted)       :: 93 of 242
            Pseudo Genes (incomplete)         :: 166 of 242
            Pseudo Genes (internal stop)      :: 53 of 242
            Pseudo Genes (multiple problems)  :: 62 of 242
            Pseudo Genes (short protein)      :: 4 of 242
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP032071.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4110
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="E166"
                     /host="Avian"
                     /db_xref="taxon:562"
                     /plasmid="p.E166-P5"
                     /geo_loc_name="China"
                     /collection_date="2014"
     gene            171..461
                     /locus_tag="D2J89_RS25565"
                     /old_locus_tag="D2J89_27705"
     CDS             171..461
                     /locus_tag="D2J89_RS25565"
                     /old_locus_tag="D2J89_27705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000091756.1"
                     /translation="MSRTLEQKIAEAEARLQRLKAKSRSLDTAQKVVVGAALLAKVRKP
                     EEVQLRAWLLQFLKAEVTRQADVSRIQPLIDELNALPKPVPKGVSKNGQQA"
                     /name="hypothetical protein"
     gene            993..1694
                     /locus_tag="D2J89_RS25570"
                     /old_locus_tag="D2J89_27715"
     CDS             993..1694
                     /locus_tag="D2J89_RS25570"
                     /old_locus_tag="D2J89_27715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_000227989.1"
                     /translation="MVDLIAYKSNALVEASYKLTLQEQRFLLLCISRLQSGVDAPPPEL
                     QKTMTITAVEFFNSFPDMGRNHAEEKLQEAIDRLWDRSIILKDGEKREEFRWIQYRAQY
                     LKGEAKVQITFADAVMPYLTQLKGQFTKVVIKNISNLSKSYSIRIYEILQQFRNTGERM
                     ISLDDFRSMLQLEDKYKDFKTLNRALIKPAVAELNQKSDLAVTVETIKKGRTVVALHFR
                     FKEDKQIKMTI"
                     /name="replication initiation protein"
     gene            complement(1944..4103)
                     /gene="mobQ"
                     /locus_tag="D2J89_RS25575"
                     /old_locus_tag="D2J89_27720"
     CDS             complement(1944..4103)
                     /gene="mobQ"
                     /locus_tag="D2J89_RS25575"
                     /old_locus_tag="D2J89_27720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_052940773.1"
                     /translation="MAIYHLSMKIISRSNGYSAVASAAYRSGSLMLDERTGLTHDYTRK
                     SGVAEAVILSPATAPAWCTNRTELWNAVEKAERRKNSQLAREIELAIPRELPQDAARET
                     VLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAVGPAGFGGKVRDWNDRTHAET
                     WRASWADHANRALANAGYQEEIDHRSYERQGLEKTPGIHLGKSACAMETRGIETERGEQ
                     NRLINRLSLEIQIFRTELRNSGLTEPARRVADLLNIELPDNATSYTLRDIIEALPKDSN
                     AAWKLTSNAMSMMTDMQATRRRWEELNTERKEAMSHAASLKKSSPFSSGFSRIPLMQWA
                     APEYRSEQGKIQSLSQQMEKLRQHYRKVEKQDIPASQTEFETQWQQWGASGLAALRAQL
                     LKREEELRRKELEDTERSRAERRAEQLRKNDNAVMDSGVLRAVVTGYGEAPMPGNGEMT
                     SYLLLHNRNGEYTLWGNELEKYRTRIFERVDLMRDRSGYICDRSEIGQHPPLQRVYSSA
                     TFEQLLTQVCQTWPQHTRNLRQPKTWPEFFCLGEDRQPAMPSLAARKVDFTQGRLPPTL
                     MPVMSSVDRETRQLQLLLVMGVDDSLGGVVRLNGTLYPAFAVPSADNRHLVISALTDKG
                     LRYAGYGVAVNHDADSHISPAPELMEFHLKTREAPLFAAVNTPEKQPDHLFRSLGFNRT
                     WDEWRREEDARTHATERRHDRGWSQ"
                     /name="mobQ"
     Typing          2..170
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="94.152"
                     /coverage="96.0"
                     /evalue="7.47e-67"
     Typing          complement(3746..4104)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="97.222"
                     /coverage="100.0"
                     /evalue="3.97e-172"
     Typing          complement(1948..4109)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.365"
                     /coverage="96.0"
                     /evalue="0.0"
ORIGIN
        1 gtaatgcctt tttgcagttt tctttcagcc ccgcagggcg cacacaatcg aaccgaaggt
       61 gagattgtat aagtgcgcac ttcgtgattt ctgcgcatta tgccagctca gtttttaaac
      121 taccagtgac gagctaaaat gcgatttttc accgcgcacg gagttacatc atgagcagaa
      181 cactcgaaca gaaaattgcc gaagctgaag ccagattgca gcgtctgaag gcaaagagcc
      241 ggagcctgga cacagcgcag aaggtcgttg taggtgctgc attgctggca aaggtcagaa
      301 agccggagga ggtgcaattg cgcgcctggt tactccagtt cctgaaggca gaggtgacac
      361 ggcaggctga tgtgagcagg atacagccgt tgattgacga actgaacgcg ttgccaaaac
      421 ccgtccccaa aggcgtgtca aaaaacggcc agcaggcgtg aatgcaccat ccgctcgccg
      481 cagccccagc gaccgagcgt tagcgagcga gtgggcgagg aagcggaaaa gctgctgcac
      541 caaaacaggg cacttacgcg ccgatgctca gttggtgata gccgttcaac ccgcatgaat
      601 actggttttc cacagggttg aacagcgttt tgccagtgtc tgctgttctg cttttttgta
      661 acttactgtt tttcatgggt ttttgttttg cgcaccccga gttttccaca ggctcgccgt
      721 taagttttta gatcttgttt ttaagatctt gtttttaata cttgttttcg gaccgaaatt
      781 tcttatggaa ttcaagggat tagattgcta taggtgaaca tttcgggggt aaaggtgaac
      841 atttcggggg tggttaggtg aacatttcgg gggtaaaggt gaacatttcg ggggtggtta
      901 tccacagaaa atggtgaatt aaaaaaaaat gattgcacca attgatgggg tgaactagtt
      961 tttttttagt ttactttagg tgagggtgct caatggttga tttgatagct tacaaatcca
     1021 atgccttagt cgaagccagc tacaagctca cgttacagga gcagcgtttc ctgttgctgt
     1081 gcattagtcg attacagtca ggtgttgatg ctcctccacc tgaattacaa aagaccatga
     1141 ctattacggc tgtggagttt tttaattcat ttcctgacat gggaagaaac catgccgaag
     1201 aaaagttaca ggaagcgatc gatcgtctgt gggacaggtc gatcattctt aaggatggtg
     1261 aaaaacgcga ggagttccgt tggatccagt atcgggcgca gtacctgaaa ggcgaagcaa
     1321 aagttcaaat aactttcgca gatgctgtaa tgccgtactt aacgcaatta aaagggcaat
     1381 tcacaaaagt tgtaattaaa aacatatcta atttatcaaa gagttatagc atcagaatat
     1441 acgaaatcct ccaacagttc cgtaacacag gggagcggat gatttctcta gatgatttcc
     1501 gcagcatgct acaactggaa gataaatata aagatttcaa gacgttaaat agagccctca
     1561 taaaaccggc agtggcggaa ctgaatcaaa aaagcgatct ggccgtaacg gttgagacaa
     1621 tcaagaaggg gcgcactgtc gtagccctgc attttcgctt caaggaagac aaacagatca
     1681 agatgacgat ctaaaatcga ttctaaggca tctaaaacgc gcgctgacgc gttttttttg
     1741 tgtaaggtat gcgatggtac tacgaatcac aaaaacatcg tcagaatcgc tctcacgagc
     1801 taaaaaaatc ccccatatgc ggggggaaga aactatgcgg agattatcta gtttgttgtg
     1861 tgtgcttatt gacgttcatc cctgtcatgt gaacgtcaca tgaacataac atgatgttaa
     1921 acaaaacaaa atattgtgca cattcactgt gaccaaccgc gatcgtgcct cctctcggtg
     1981 gcgtgtgttc gtgcatcttc ctcgcgccgc cattcgtccc aggtacgatt aaaacccagc
     2041 gagcggaaca gatgatccgg ctgtttttct ggtgtattga cggcagcaaa aagcggtgcc
     2101 tcacgggttt tcagatgaaa ctccataagt tccggtgcag gtgatatgtg gctgtctgca
     2161 tcgtgattga cggcaacacc ataccctgca taccgtaacc ccttgtccgt cagcgcactg
     2221 atgaccagat gcctgttatc ggctgaggga acggcaaacg cgggatacag cgtgccatta
     2281 agccggacaa cgccgcccag agagtcatcc acgcccatga ccagcaaaag ctgaagctgg
     2341 cgggtttccc ggtcgacaga actcattacc ggcatcagcg tcggtggcag acgtccctgc
     2401 gtaaaatcca ccttccttgc tgccagtgat ggcatggctg gctggcggtc ttctccaaga
     2461 cagaaaaact ccggccatgt cttcggctgg cggagattgc gggtatgttg tggccatgtc
     2521 tggcaaacct gcgtcagcag ttgctcaaag gtcgcagagg aataaacacg ttgcaatggt
     2581 ggatgttgac cgatttcgct tcggtcacag atataaccac tcctgtcacg cattaaatca
     2641 acacgctcaa agattctggt gcggtatttc tccagctcgt tcccccagag cgtatattct
     2701 ccgttccggt tgtgaagcag aaggtaactg gtcatttccc cgttaccagg catgggggcc
     2761 tcgccatacc ccgtaacgac tgctctcagt acccctgaat ccataacggc attgtcattt
     2821 ttcctgagct gctccgctcg tcgctccgcg cgactacgtt ctgtatcctc cagctccttc
     2881 cggcgcaatt cctcttcacg tttaagcaac tgggctctga gagctgcaag gccggatgcc
     2941 ccccactgct gccactgtgt ttcaaattcc gtctgactcg cgggaatgtc ctgtttctcc
     3001 accttccggt aatgctgtcg caatttttcc atttgctggc tcagagactg aatttttccc
     3061 tgctcactac ggtattccgg tgcagcccat tgcatcaggg gaatacggga aaaaccggac
     3121 gaaaacggcg atgatttttt caggctggca gcatggctca tcgcttcctt tcgttcggtg
     3181 ttcagctctt cccagcgtct gcgcgtcgcc tgcatatctg tcatcatcga catggcatta
     3241 ctggtgagtt tccatgccgc gttactgtcc ttcggcaacg cttcgatgat gtcacgtagt
     3301 gtgtacgaag ttgcgttgtc aggaagctca atattcagaa ggtcagccac acggcgggca
     3361 ggctctgtca gcccgctgtt cctgagttcg gtacggaaaa tctgtatttc caggctaagc
     3421 ctgttaatca gacgattctg ttcgccgcgt tctgtttcga taccgcgtgt ttccatcgca
     3481 caggcagact ttccgaggtg aataccaggc gttttctcaa gcccctgtcg ctcgtatgaa
     3541 cgatggtcta tctcttcctg ataaccggcg ttcgccagtg ccctgtttgc atggtcagcc
     3601 catgatgccc gccacgtctc ggcatgtgtc cggtcgttcc agtccctgac ctttccaccg
     3661 aaccccgcag gtccgacagc cctcgtggtc agcatgatgt gcgcatgggg atttgtttta
     3721 tccatgtgat ggaacgccac atcagcaatc atgccctgac tgacaaagtt ttctctgaca
     3781 aaggcgagaa cggtttcccg tgcggcatcc tgcggcagtt cacgggggat ggcgagttca
     3841 atttcccgcg caagttgtga gttcttccga cgttcggcct tctcaacggc attccagagt
     3901 tcggtacggt tcgtacacca ggcaggggcg gtggcggggg acagaatcac agcctcagcc
     3961 acaccgcttt tgcgggtgta gtcgtgagtc agtccggtac gctcatcaag cattagtgaa
     4021 ccggaacgat aggcagcaga agcaacggcg ctgtagccgt tgcttctgga gatgattttc
     4081 atgctgagat gataaatcgc catttctgcc
//
