LOCUS       NZ_CP032004            10694 bp    DNA     circular CON 09-JAN-2024
DEFINITION  Acinetobacter haemolyticus strain 11616 plasmid pAhaem11616e,
            complete sequence.
ACCESSION   NZ_CP032004
VERSION     NZ_CP032004.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN09927968
            Assembly: GCF_009892385.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter haemolyticus
  ORGANISM  Acinetobacter haemolyticus
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter.
REFERENCE   1  (bases 1 to 10694)
  AUTHORS   Castro-Jaimes,S. and Cevallos,M.A.
  TITLE     Analysis of the genomic diversity of Mexican Acinetobacter
            haemolyticus clinical isolates
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10694)
  AUTHORS   Castro-Jaimes,S. and Cevallos,M.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-AUG-2018) Evolutionary Genomics Program, Center for
            Genomic Sciences, National Autonomous University of Mexico (UNAM),
            Av. Universidad s/n Col. Chamilpa, Cuernavaca, Morelos 62210, Mexico
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: FEB-2018
            Assembly Method                   :: Unicycler v. 0.4.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 483x
            Sequencing Technology             :: Illumina HiSeq and PacBio RSII
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/09/2024 02:29:05
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,488
            CDSs (total)                      :: 3,393
            Genes (coding)                    :: 3,215
            CDSs (with protein)               :: 3,215
            Genes (RNA)                       :: 95
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 43 of 178
            Pseudo Genes (incomplete)         :: 137 of 178
            Pseudo Genes (internal stop)      :: 26 of 178
            Pseudo Genes (multiple problems)  :: 25 of 178
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP032004.1.
            Bacteria available from Submitting center.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10694
                     /organism="Acinetobacter haemolyticus"
                     /mol_type="genomic DNA"
                     /strain="11616"
                     /isolation_source="Blood culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:29430"
                     /plasmid="pAhaem11616e"
                     /geo_loc_name="Mexico: Mexico City"
                     /collection_date="2012-02-03"
                     /collected_by="Instituto Nacional de Cancerologia"
     gene            1..951
                     /gene="repM"
                     /locus_tag="Ahae11616_RS17185"
                     /old_locus_tag="Ahae11616_17325"
     CDS             1..951
                     /gene="repM"
                     /locus_tag="Ahae11616_RS17185"
                     /old_locus_tag="Ahae11616_17325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004786597.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepM"
                     /protein_id="WP_060513885.1"
                     /translation="MRELVVKDNALINASYNLDLVEQRLILLAIVEARESGKGINANDP
                     LTVHAESYINQFGVHRNTAYQALKDACNDLFARQFSYQKINERGNIENYRSRWVSEIGY
                     VDNEAVVKLIFAPAIVPLITRLEEHFTKYELQQVSNLSSAYAVRLYELLIAWRSTGSTP
                     IIELSDFRQRIGVLDTEYKRMERFKTSVLELAIKQINEHTDIIVKYEQHKRGRSISGFS
                     FNFKQKKKDSLSIERVPNTLDLSSKMTDAQRHMFANKLSELPEMGRYSQGTESYPQFAV
                     RIAEMLQDSEKIKELAPYLKKVGYMPSNKKDTVNG"
                     /name="repM"
     gene            944..1519
                     /locus_tag="Ahae11616_RS17190"
                     /old_locus_tag="Ahae11616_17330"
     CDS             944..1519
                     /locus_tag="Ahae11616_RS17190"
                     /old_locus_tag="Ahae11616_17330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005102333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication DNA-binding protein"
                     /protein_id="WP_060513917.1"
                     /translation="MAKLSLSEVSKKFHIDRSTIYRAVRNGRLSRSSDGQFDLSEVIRC
                     FGEPEQTSQQIESSTSDSDESTKKLIIHLENEVKKYQEREERLMQQIDRMQTLIELKTV
                     APATAAPQQDATACDTDMPQHATTQQGNDNKKNNELNIVENVAVPQQETTAHHSQTLQH
                     ATLQNVAVPQPKKRGLFGRVLNAVFDND"
                     /name="plasmid replication DNA-binding protein"
     gene            1927..3243
                     /locus_tag="Ahae11616_RS17195"
                     /old_locus_tag="Ahae11616_17335"
     CDS             1927..3243
                     /locus_tag="Ahae11616_RS17195"
                     /old_locus_tag="Ahae11616_17335"
                     /inference="COORDINATES: protein motif:HMM:NF024573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="WP_060513878.1"
                     /translation="MLSKIRILNYKSLNDIYFDPTHVNLFLGENGAGKSNILEALAMYS
                     AAKSNMLSNEFLISRGIRPIDPLTTLSQFPLVEKDKSDNEERSKNFAIFTQDNLLPIGV
                     AICHDEEDPYRGLKAKIFTARFNENDDGDGEKSYLIDENIEESHSKVMNDLFKSFSEIN
                     KLFPQVEDINEIYKEKFIKELKKKDKNKISKLLEIQNKMHYLSENLKLIDGFRSKKSSK
                     DNFVIFNPEINTLMGENTQSQIQPLGIHGEGLFTLLKVMAKKEPENFKDVIDTASIFNW
                     LEKIELVEDLNEQKIVLKDRFMDGYISPKSANEGFLFSLFYACLFCSKQTPSVFAIESI
                     DKSLNPRLCQVLVKKLVEKAKKYNKQVFLTSHNAAVLDGMDLLDKDQSIFIIERATSGE
                     TKIRKLTEEHLPKPKRNGEKLNLSEAFLRGMLGGLPSNF"
                     /name="AAA family ATPase"
     gene            3258..3929
                     /locus_tag="Ahae11616_RS17200"
                     /old_locus_tag="Ahae11616_17340"
     CDS             3258..3929
                     /locus_tag="Ahae11616_RS17200"
                     /old_locus_tag="Ahae11616_17340"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_060513879.1"
                     /translation="MRFSIIGEGSTDFRVLKTILESFFVDSEINSLVPVLDVSTLKTAK
                     DEHGSWTKIPEYLKSNFLEDALVNTDYLVIQIDTDVCEEPGFDVSPKVLADSNQEDFYN
                     LVQAKLIEWIDSFEPDTYNYYQEKFIFAIAIHSLECWLIAYYCKSSELKIKRIKNGFNH
                     LQRALERDKKIHLAEPKDPAVYEELAIPFRKRKHHAQARTYSYSLNRFMCQLEEIESKM
                     V"
                     /name="hypothetical protein"
     gene            complement(4017..4628)
                     /locus_tag="Ahae11616_RS17205"
                     /old_locus_tag="Ahae11616_17345"
     CDS             complement(4017..4628)
                     /locus_tag="Ahae11616_RS17205"
                     /old_locus_tag="Ahae11616_17345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004889724.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_004740252.1"
                     /translation="MIEMNTRIYLRASTKDQDAERALQILQDLNQNLNLGETIVYVENY
                     SGTKLDRPELNKLLSEANQGDTLLVESIDRLSRLTQRDFQELKRRIQEKGLRLVVADLP
                     TTYQMIQTSDSITHSILELINNMLIDLLATMARLDNEKRIERIKQGLARSGYKPTGKKA
                     NEAKHKRIKELLAAGNMTKEEIAKAVNCGVATVYRVAKVI"
                     /name="recombinase family protein"
     gene            4843..5370
                     /locus_tag="Ahae11616_RS17210"
                     /old_locus_tag="Ahae11616_17350"
     CDS             4843..5370
                     /locus_tag="Ahae11616_RS17210"
                     /old_locus_tag="Ahae11616_17350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000537572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid pRiA4b ORF-3 family protein"
                     /protein_id="WP_125305375.1"
                     /translation="MGTVKLKSSDEVFQLKISLLGSDPEIWRTIQISSAATLSTLHKAI
                     QSAFDWEDSHLHEFTTIKHEKINKRQKLKEVLRVGKKIIYTYDFGDCWEHLITVESRQS
                     PDLNKKYPCCIDGQNHAPFEDIGGIFGYLDILDALQDAKHPRYDDYMQIIEDEIGEIEF
                     DPTYFNSHDIKF"
                     /name="plasmid pRiA4b ORF-3 family protein"
     gene            5611..5898
                     /locus_tag="Ahae11616_RS17215"
                     /old_locus_tag="Ahae11616_17355"
     CDS             5611..5898
                     /locus_tag="Ahae11616_RS17215"
                     /old_locus_tag="Ahae11616_17355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019383542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="WP_060513954.1"
                     /translation="MEQYFEWDEAKNRKNQKKHDVSFETASLVFDDPLRISMQDRHTDG
                     EERWQTIGRVKGVLMLLVAHTIFDEDDCEIIRIISARQATKAERDRYEHG"
                     /name="BrnT family toxin"
     gene            5885..6199
                     /locus_tag="Ahae11616_RS17220"
                     /old_locus_tag="Ahae11616_17360"
     CDS             5885..6199
                     /locus_tag="Ahae11616_RS17220"
                     /old_locus_tag="Ahae11616_17360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006582675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="WP_060513955.1"
                     /translation="MSTVRYSRKELNEKFSEKQNAEIQRLLAKGTVPDDQLDLSDIPEI
                     TDWSNAVRHNQFYRPVKQQTSIRLDADVLAWFKSQGKGYQTRMNEILRDAMLKELKNHQ
                     "
                     /name="BrnA antitoxin family protein"
     gene            6426..6743
                     /locus_tag="Ahae11616_RS17225"
                     /old_locus_tag="Ahae11616_17365"
     CDS             6426..6743
                     /locus_tag="Ahae11616_RS17225"
                     /old_locus_tag="Ahae11616_17365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005065053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004728121.1"
                     /translation="MLGQHHHFLKKLSISDAAIAYRKPRSTILKYIKSGDFELDYNNLI
                     EYSQLVKKLGEITSPIRDERDQQIEKLKDEVMHLKKQLNFFKYTSISNKHRLLISHKDS
                     I"
                     /name="hypothetical protein"
     gene            complement(6983..7207)
                     /locus_tag="Ahae11616_RS17230"
                     /old_locus_tag="Ahae11616_17370"
     CDS             complement(6983..7207)
                     /locus_tag="Ahae11616_RS17230"
                     /old_locus_tag="Ahae11616_17370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004676219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004758944.1"
                     /translation="MPYTEFQRLVGKAGLSIKEFAALLDMKPNSITNYSKQGVVPTHIA
                     VIVALISTMKDEGLDFYPIFEKVKSYSQE"
                     /name="hypothetical protein"
     gene            7361..7606
                     /locus_tag="Ahae11616_RS17235"
                     /old_locus_tag="Ahae11616_17375"
     CDS             7361..7606
                     /locus_tag="Ahae11616_RS17235"
                     /old_locus_tag="Ahae11616_17375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005249014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_005249014.1"
                     /translation="MYSYPDSNTEKKIALMIINDFFIQKAHELWIFLQLDQSFNDYEAT
                     LIWTRRYLEEHPEGEYSDIQKAFLSCFPEHFFSFDC"
                     /name="hypothetical protein"
     gene            complement(7892..8653)
                     /locus_tag="Ahae11616_RS17240"
                     /old_locus_tag="Ahae11616_17380"
     CDS             complement(7892..8653)
                     /locus_tag="Ahae11616_RS17240"
                     /old_locus_tag="Ahae11616_17380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017393773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_057073610.1"
                     /translation="MEMEKAKKRGRPAQLLQIAELHAFVEFLEQQEQLSDLQSQVLKAL
                     NSVDCNFEGLTQTDQVLVKEALKPYREHLKLKLLFEELNNLPLKTEYEQKFLDLYELFQ
                     KNALDQMELNILKTLATRYLNFKAQKLEYSDLELYLSQLQKKDAGKKRKAENQRKFELG
                     GAVLVAFKKLNIDISNDTPQQITNRIVNTTKLHNEVRKSLIFKDVKTYKNEYFEANKLF
                     IQVLEGLHTWQKGGELLSVIEIKKALEKGEE"
                     /name="hypothetical protein"
     gene            8956..10125
                     /locus_tag="Ahae11616_RS17245"
                     /old_locus_tag="Ahae11616_17385"
     CDS             8956..10125
                     /locus_tag="Ahae11616_RS17245"
                     /old_locus_tag="Ahae11616_17385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002011489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_125305376.1"
                     /translation="MATYHFSVKSKGKGYAIQHYLYIARLMQYENVRKKSNEILEYVEP
                     GKCMPSWVKDPIEFWQAADTFERENAKVYVEYEIALPNEFTPEQRKTLIETFLDKHIVP
                     QQYPHSYAIHNVKSRLSGEDQPHCHIMFSLKADDGIERTAEQYFKRYNPKDPSKGGAKK
                     IQLQDGHADYSTFLIYIRKQWENHLNDALAQHCPTVTYHLDGQDITIKNQVSADSYEKY
                     NDIHGTLYLPEPKLGVGKQNATPEYLAEIQKIREHNQQERELEERQKRLDQQHEYFYSA
                     ENHPFSSLEVMYYLNYLVKNQKNKEKLQKDFSEIIKTQEPILQQEDWNIHNMNQKLQNS
                     KHNSTPCFEFDVYSPPKNPLPTQNKPSKLVQSEQPKPKSDDYDYGGPGF"
                     /name="MobA/MobL family protein"
     Typing          2..951
                     /name="rep_cluster_1172"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.437"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          8957..10122
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.602"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atgagagaat tagttgtaaa agacaatgcc ttaattaatg caagctataa cttagattta
       61 gtagaacaac gtttaatttt attagctatt gttgaggcaa gagaaagtgg gaaagggatt
      121 aatgctaatg atccattaac tgtccatgca gaaagctata tcaatcaatt tggcgtacat
      181 cgcaataccg cttatcaggc attaaaagat gcctgtaatg atttattcgc aagacaattc
      241 agctatcaaa aaataaatga aagagggaat attgagaact atagatcccg ttgggttagt
      301 gaaattggat atgtagataa tgaagcagtg gttaagctta tttttgcccc tgccatagtt
      361 ccattaatta cgcgcctaga agagcatttt actaaatacg aattgcagca agttagtaat
      421 ctcagtagtg cctatgctgt tcgtttatat gagttattaa ttgcttggag aagtacgggc
      481 tctactccaa ttatagagct aagtgatttc cgtcaaagaa ttggcgtact cgatactgag
      541 tacaaacgta tggaacgctt taaaactagt gtacttgagc tcgctattaa acaaattaac
      601 gaacatacag atatcatagt gaagtatgaa caacacaaaa gaggtcgatc aatttcagga
      661 ttttctttta actttaaaca gaaaaaaaag gacagcctat caatagaaag agttccgaat
      721 actttggatc tttcttcaaa gatgaccgat gctcaacggc atatgtttgc aaataaactt
      781 tcagaactcc ctgaaatggg tcgctattca caaggaactg aaagctatcc tcaatttgct
      841 gttcgtattg ctgagatgct acaagactct gaaaaaatca aagaactagc cccataccta
      901 aaaaaagtgg gatacatgcc atcaaataaa aaggacacag taaatggcta agttatcgct
      961 aagtgaagta tctaaaaaat ttcatattga taggtcaacc atttacagag ctgtacgtaa
     1021 tgggcgttta tcacgctcta gtgatggtca atttgacctt tcggaagtga ttagatgctt
     1081 tggggagcct gagcaaacat ctcaacaaat tgaatcttct acatcagata gcgatgaatc
     1141 tacaaaaaaa ctcattattc atttagaaaa tgaagttaaa aaataccaag aacgtgaaga
     1201 gcgtttaatg cagcaaatcg accgtatgca gacgcttatt gagttaaaaa ctgttgcacc
     1261 cgccacggca gcgccacaac aagatgctac ggcatgcgac accgatatgc cacagcatgc
     1321 gacaacacaa caaggcaatg ataataaaaa gaataatgaa ttaaatattg tagaaaacgt
     1381 ggcagtgcca cagcaagaaa ctacagcaca ccacagccaa acgctacagc atgccacgtt
     1441 gcaaaatgtg gcagtgccac aacctaaaaa acgtggctta tttggccgtg tgctgaatgc
     1501 tgtttttgat aatgactaag ggagagagat catgccgaaa ctgaaagaca ttgccctggg
     1561 aattattgtg gccccgctgc tgatcccgat catgctgatt gcatcgtacc aggataaaaa
     1621 ggcactcaaa aaagagctgg attaacgcca aaaagaaaaa gaccaggcat cctgattatt
     1681 caattaacct aaacaaagtc gtgaaacatt gcttaaataa taggcgtgga acgtgaaaaa
     1741 tcataaaaaa agcccacctt ggggaaggtg ggctatcaac tagaaactac agcattgatt
     1801 tctaagtgga attataacgc atttcgtata aggtgtatta tgttaatttt agcatctctt
     1861 aaaagttcct atctagctct gtgttcatgc taatatcatt gaaacttatc tttatgacta
     1921 tctgaaatgc tttctaagat tcgaattctt aactataaat cactaaatga tatttacttt
     1981 gatccaactc atgtaaatct atttttaggt gaaaatggtg ctggaaaatc taatatttta
     2041 gaagctttag caatgtatag tgctgcaaaa tcgaatatgt tgagtaatga attcctgata
     2101 tcaagaggaa tacgcccaat agatccatta acaacactta gccaattccc tttggtagaa
     2161 aaagataaga gtgataacga agaacggtct aaaaattttg caatttttac ccaagataat
     2221 cttctaccta tcggggtagc tatctgccat gatgaagaag atccatatcg tggtttaaaa
     2281 gctaaaatat ttacagctag attcaatgaa aatgatgatg gtgatggtga aaaaagttat
     2341 ttaatagatg agaatattga agaaagccat agcaaggtaa tgaatgattt attcaaaagc
     2401 ttttctgaaa tcaataaact attccctcaa gtagaggata ttaacgagat ttataaagaa
     2461 aaatttatta aagaattaaa aaagaaagat aaaaataaaa tttctaagtt attagaaatt
     2521 caaaataaaa tgcattattt aagtgaaaac ttaaaactta tagatggttt cagatcaaag
     2581 aagtcatcta aagataattt tgttattttc aaccctgaaa taaacacact aatgggcgag
     2641 aacactcaat cacaaattca gccattagga atacatgggg aaggattatt caccctgtta
     2701 aaagtcatgg caaaaaaaga acctgaaaac tttaaagatg taattgatac agcttcaatt
     2761 ttcaattggt tagaaaagat agaactggtc gaggatctaa atgaacaaaa aattgttctc
     2821 aaagatcgct tcatggatgg atatatttcc cctaaaagtg ctaatgaagg ctttctattt
     2881 agcttattct acgcctgtct cttttgctca aaacaaactc catctgtatt tgcaatcgag
     2941 agtattgata aatcacttaa tccacgttta tgccaagtct tagtaaaaaa attagttgaa
     3001 aaggcaaaaa agtataataa acaagtcttc ttaaccagtc ataatgcagc agtgctagat
     3061 ggcatggatt tacttgataa agatcaatct atttttatta ttgaaagagc taccagtggt
     3121 gaaactaaga ttagaaaatt aacagaagaa cacctaccta agcctaaaag aaatggtgaa
     3181 aaattgaatc tttcagaagc ctttttaaga ggcatgctag gcggattacc ttcaaatttc
     3241 taattggtat aaaaattatg cgtttttcta ttattggtga aggtagtaca gattttcgag
     3301 tattaaagac tattctagaa tcattttttg tagactctga aattaactct ttagtgcctg
     3361 tcttagatgt ctcgacatta aaaacagcga aagatgaaca tggtagttgg actaaaattc
     3421 cagagtattt aaaatcaaac ttccttgaag atgcattagt gaatacagac tatttagtta
     3481 ttcaaattga cactgatgtt tgtgaagagc ctggttttga cgtaagtcct aaagttctag
     3541 ccgatagcaa tcaagaagat ttctataatt tagtacaagc taaattgata gaatggattg
     3601 atagttttga acctgatacc tataactatt atcaggaaaa gtttatattt gcgattgcga
     3661 ttcactcttt agaatgttgg ttaatcgcat attattgtaa aagttcagaa ctaaaaatta
     3721 aaagaattaa aaatggtttt aatcatctgc aaagggcttt agaaagagat aaaaaaatcc
     3781 acttagcaga gccaaaagat cctgctgtat atgaagagtt ggcaattcct tttagaaaaa
     3841 ggaagcatca cgctcaagca agaacctatt catatagttt gaatagattc atgtgccaat
     3901 tggaagaaat agaaagtaaa atggtctaaa catttaacat aaaatctctt atgtgaagca
     3961 atcttgtaac tccaaaatag aaatgtccgg tttctccaaa gtaaaaatgt ccgcttttag
     4021 ataactttag caactcgata gacagttgca actccacaat tcactgcttt ggcaatttct
     4081 tccttagtca tattgccagc tgctagcaat tcttttattc gtttgtgctt agcctcattg
     4141 gctttcttac ctgttggctt gtaacccgaa cgtgccaggc cctgcttaat acgttctata
     4201 cgtttctcat tgtctaggcg agccattgtt gccaggagat caatcaacat attgttgatg
     4261 agttccagga ttgaatgagt aatgctgtca ctggtttgaa tcatttggta ggtagtagga
     4321 agatccgcta cgactaaacg aagtcccttc tcctggatcc tgcgcttcag ctcttgaaaa
     4381 tctcgttggg ttaggcgtga taagcggtca atactttcaa ctaacaaggt gtcaccttga
     4441 tttgcttctg acagcagctt attcaattca ggtcggtcta acttcgtacc actatagttt
     4501 tccacgtaca caatggtttc acccaaattc aagttttggt ttagatcctg aagaatctgc
     4561 aaagcccttt ctgcatcttg atctttagtt gaagctcgta gataaatacg tgtattcatt
     4621 tctatcattt aatcttaatt ctattagatt aatgataaca atatcattta attattgcat
     4681 taagtctaat gatagatatt gtatgcgatt tggctcgcta tcattcagct atactctttt
     4741 gatagaaaaa tcatgacaga agggcatgct tggtaagttc atataacgtg tattatgtta
     4801 attttagatt tactaaaact attttttgag aactaggctg ttatgggaac tgtaaagctc
     4861 aaatcttctg atgaagtttt tcaattaaaa attagtcttc taggcagtga tcctgaaatc
     4921 tggagaacta ttcaaatttc atcggcagct actttatcta cgctccataa ggcaattcaa
     4981 tctgcttttg attgggaaga tagccattta catgaattca cgactattaa gcatgaaaag
     5041 atcaataaaa gacaaaaatt gaaagaagtt ctacgtgtag gtaaaaaaat tatatataca
     5101 tatgattttg gcgattgttg ggaacatttg attactgtag aaagcagaca atctcctgat
     5161 ttaaataaaa aatatccatg ttgtatagat ggtcaaaatc atgctccatt tgaagatatt
     5221 ggaggaattt ttggatatct tgatatttta gatgcattac aagacgccaa acatccaaga
     5281 tatgacgatt acatgcaaat tatcgaggat gaaataggag agatagagtt tgatccgacc
     5341 tactttaatt cacatgatat taagttttaa acctgattta acataatggc ggttatacga
     5401 agtacatttg tatattaatt taaatatcaa ctacttaata aaaatataaa aatccaaaaa
     5461 tgcccaaaaa gccgattttg aaacaagtcg gcttttttgt gagcagtttt ggaatgtttt
     5521 gccaataaat ttgactaaaa attacgcttc tcaacttgag tgatacaatt ttgtgataca
     5581 ttattgtata tacaaatttg aatgtttttt atggaacagt atttcgaatg ggatgaggca
     5641 aagaatcgaa agaatcagaa aaaacatgat gtctctttcg aaacggcaag ccttgttttt
     5701 gatgatccat tacggatctc aatgcaggat agacataccg atggtgaaga acgttggcaa
     5761 accattggaa gagtaaaagg cgtactaatg ctattagtag ctcacaccat cttcgatgaa
     5821 gatgattgtg aaatcatacg aatcattagt gcaagacaag ccactaaggc ggagcgagat
     5881 agatatgagc acggttagat actcacgcaa agaactgaat gaaaaattta gtgagaagca
     5941 aaatgctgaa attcaacgct tgcttgctaa aggcacggtt cctgatgatc agctagatct
     6001 atcagatatt cctgaaatta ccgactggag caatgctgta cgccataatc agttctaccg
     6061 tccagtgaag caacagactt ccattcgttt agatgctgat gtacttgcat ggtttaaatc
     6121 tcaaggcaaa ggttatcaaa cacgaatgaa tgaaattctg cgagatgcta tgctgaaaga
     6181 attaaaaaat catcaataaa aatgggagct taagctccca tttttgactt cgcataacgt
     6241 gtattatgtt aataatataa attcttaaaa gcgtattgat ttaataatag aaaaattatc
     6301 agagtagggt atgtgtaaag taaacgttca attatcaacc aataatgatt gaagatttat
     6361 atcttacatt tgttaatttt attaatatat atctaccaaa ttaataatgt cttaagttaa
     6421 ataatatgtt aggtcaacat catcattttt taaaaaaact ttcgatcagt gatgctgcta
     6481 tagcgtatag aaaacctcgc tcgactatac ttaaatatat aaaaagcgga gatttcgaac
     6541 ttgactacaa taacttaatt gaatatagcc agttagttaa aaaattgggt gaaattactt
     6601 cccctatacg ggacgaaaga gatcaacaaa tcgaaaaact aaaagatgaa gttatgcatt
     6661 taaaaaaaca acttaatttt tttaaatata cttcaatctc aaacaaacat agattattaa
     6721 tttctcataa agactccatt taacataata gagcttatgc gaaattcagt tgtaatcccc
     6781 aaatagaaat gtccggttta ccgattgctc gacctaagat ctcaaaacat aagaatttga
     6841 tgatcaccct ggttctcaaa actcgatact ttattgaaaa gttctatcga ggcttataag
     6901 tccctgaaac aaaaaaatgc ctgaagttca ggcatttttt tcgtcatcac taacagctta
     6961 aaacaatcaa aaaacgagag ttttattctt gtgaatacga cttaactttt tcaaaaattg
     7021 gatagaaatc taatccctca tctttcatcg tagaaatcaa cgcaacgatt acagcaatat
     7081 gtgtaggaac cacaccttgt ttactgtaat tcgtaatcga attaggtttc atatccaata
     7141 aggcagcaaa ttccttaatt gaaagccctg ctttaccgac aagacgttga aattcagtat
     7201 atggcattta attaaattta tagaaatggt gttttgagat tatagcatag ggttatattt
     7261 acataaaaac acataaaatt atgtggcaaa cacatatttt tgtgtgttat aaatagctta
     7321 tcaactagcc tatttatcct tactatcgga gaaatgtaat atgtactcat acccggattc
     7381 aaacactgaa aagaaaattg cgcttatgat catcaatgac tttttcattc aaaaagccca
     7441 tgaactgtgg atttttttac aactcgacca aagctttaac gattatgaag ccacactgat
     7501 ttggactcga cgttatctag aagaacatcc agaaggtgaa tacagcgata ttcaaaaagc
     7561 attcctttct tgtttcccag aacacttctt cagctttgac tgttaattta tgagaggcac
     7621 ggtaaaaatc gtaatgggta agtaaatccc aaacaacagc cagtgcatac aactggcttt
     7681 ttattgtcat ttataaatcc aagactttat ttaaacatac taaaattgat gttccacggc
     7741 ttctaaacca ttgatttaag gttgcggatt cagacatctg tgacgtcact ttagggtgta
     7801 ctctaaattt acataatgtc tctgtatacg aaattcgatt gtaagcccaa aatagaaatg
     7861 tcaaacttct ctaaaatccc tgctaaataa actactcttc gcctttttct aaggcctttt
     7921 taatttcaat tactgacaat aattctccgc ccttttgcca ggtatgtaat ccttctaaca
     7981 cctgaataaa aagtttattt gcttcaaaat attcattttt atatgtctta acatctttaa
     8041 aaattaaaga tttacgtact tcattatgaa gctttgtcgt attcacaatc cgattggtaa
     8101 tctgttgagg agtatcattg ctaatatcaa tattgagttt cttaaatgcg acaagaacag
     8161 caccacctaa ttcaaattta cgttgatttt cagctttgcg ttttttccca gcatccttct
     8221 tctgtagctg actcaaatac aattctaaat cactgtattc aagcttttgc gctttaaaat
     8281 tcagataccg tgtcgccaag gtttttaaaa tatttagttc catctgatct agtgcattct
     8341 tttgaaaaag ttcatacaga tctaaaaact tctgttcata ctcggtttta agaggcagat
     8401 tattcaactc ttcaaacagc aatttcaact tcagatgttc acgataaggt tttaaagcct
     8461 ctttgaccag aacctgatct gtctgagtta gcccctcaaa attacaatca acactattta
     8521 aagcctttaa cacttgagat tgaagatcag aaagctgttc ttgctgttct aaaaactcta
     8581 caaatgcatg taactcagca atctgtaaca actgagcagg tcgaccacgc ttttttgctt
     8641 tttccatttc catcatctgc cgccaaattt tcagaactgc aacctcttta aatatggttt
     8701 taccatattt aaagagggca gtctgaaaat aattatttaa aataaaaata aaatttcttg
     8761 aaaagaattt ttatttttat tttaaataat tcgcacttga cagtttctcc tgatcggaga
     8821 aactagtacg caatggggtg cttccccctt gacccccttc aaccgaattt ttcagccatc
     8881 caggcattcc aaatccgggc ggttttcatt ccgtttcact gcataaaaac cggtccaaaa
     8941 ctattagagg tgaacgtggc aacttatcat ttcagcgtca aaagcaaggg gaaaggctac
     9001 gcaatacagc attacctcta cattgcccga ttaatgcaat acgaaaacgt ccgcaagaaa
     9061 agtaacgaaa ttttagaata cgttgaacca ggcaaatgca tgccgtcctg ggtgaaagat
     9121 ccgatcgagt tttggcaagc cgctgatacg tttgaacgag aaaatgccaa agtctacgtg
     9181 gagtatgaaa ttgccctccc caacgaattc acgccagaac agcgtaaaac gctcatagag
     9241 acgtttttag acaaacatat agttccacag caatatccgc attcatacgc catacacaac
     9301 gttaaatcgc gcctcagtgg cgaagatcag cctcattgtc acatcatgtt ctcgctaaaa
     9361 gccgatgacg gtattgaacg gacagccgaa caatatttca aacgctataa ccccaaagat
     9421 ccaagcaaag gcggtgcaaa gaaaatccag ctgcaggatg gccacgcaga ctattccact
     9481 tttttaatct atatccgaaa acaatgggaa aaccatctga atgatgcgct tgcacagcac
     9541 tgcccgacag tgacctacca tctcgatggc caggacatca cgataaaaaa ccaagtttcc
     9601 gctgacagtt atgaaaaata caatgatatc cacggcacct tataccttcc ggaaccaaag
     9661 ctaggggttg gcaaacaaaa cgcaacgcct gaatatctgg ccgagatcca aaaaatccga
     9721 gaacacaatc agcaagagcg agaactagaa gaacgccaaa aacgcttaga tcaacaacat
     9781 gaatattttt atagtgctga aaaccatccc ttttcatcat tggaagtgat gtattacctg
     9841 aattatttag tgaagaatca gaaaaataag gaaaaactgc aaaaagattt tagtgaaatt
     9901 atcaagaccc aagaacctat attgcaacaa gaagactgga acattcataa catgaatcaa
     9961 aaattgcaga attcaaaaca taactcaacg ccatgctttg agtttgacgt gtattcccca
    10021 ccaaagaatc cattacccac gcagaataag ccctctaaac tggttcaatc tgaacagcca
    10081 aagccaaaat ctgatgatta tgattacggt ggtccggggt tttaaattat ctggatttat
    10141 tcaggcttta cagagcgagt gtctacgagc gaacagacac aattaaaatt ttcgccatgc
    10201 tttttctctg attacaagtt ccccctttgc tgctaagacg gaggtacaga gcatcccgaa
    10261 gggtgcgaac agattcaatt tgcttctagg ccgagcgaag cgagcataaa aaaatgatga
    10321 gcgaagcgaa ttccgagttg cttttgatct tgcttttgct ttttctcaaa gtcataacgg
    10381 gataagccaa aaaattgccc cgacgaactg agctttagcg aagttcaata gagtttgagc
    10441 gtagcgaaaa ccaagggcaa tttttcatga tctaggcttt taaatatttt taaatctttt
    10501 aaatgctttt agatacgctg aaagccctgt aagtaaaggc tcctagactt catatgacac
    10561 cgtttacctt gcaatatgac accgtttacc ttgcaatatg acaccgttta ccttgcaata
    10621 tgacaccgtt taccttgctt atagcactaa aattaattaa tgtgtcatag cataataaat
    10681 aatactggtg cttc
//
