LOCUS       NZ_CP031994            10554 bp    DNA     circular CON 28-NOV-2023
DEFINITION  Acinetobacter haemolyticus strain 2126ch plasmid pAhaem2126chd,
            complete sequence.
ACCESSION   NZ_CP031994
VERSION     NZ_CP031994.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN09927970
            Assembly: GCF_009892345.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter haemolyticus
  ORGANISM  Acinetobacter haemolyticus
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter.
REFERENCE   1  (bases 1 to 10554)
  AUTHORS   Castro-Jaimes,S. and Cevallos,M.A.
  TITLE     Analysis of the genomic diversity of Mexican Acinetobacter
            haemolyticus clinical isolates
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10554)
  AUTHORS   Castro-Jaimes,S. and Cevallos,M.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-AUG-2018) Evolutionary Genomics Program, Center for
            Genomic Sciences, National Autonomous University of Mexico (UNAM),
            Av. Universidad s/n Col. Chamilpa, Cuernavaca, Morelos 62210, Mexico
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: AUG-2018
            Assembly Method                   :: Unicycler v. 0.4.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 86x
            Sequencing Technology :: Illumina MiSeq and PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/28/2023 04:02:48
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,549
            CDSs (total)                      :: 3,458
            Genes (coding)                    :: 3,272
            CDSs (with protein)               :: 3,272
            Genes (RNA)                       :: 91
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 45 of 186
            Pseudo Genes (incomplete)         :: 144 of 186
            Pseudo Genes (internal stop)      :: 26 of 186
            Pseudo Genes (multiple problems)  :: 26 of 186
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP031994.1.
            Bacteria available from Submitting center.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10554
                     /organism="Acinetobacter haemolyticus"
                     /mol_type="genomic DNA"
                     /strain="2126ch"
                     /isolation_source="Ascites liquid"
                     /host="Homo sapiens"
                     /db_xref="taxon:29430"
                     /plasmid="pAhaem2126chd"
                     /geo_loc_name="Mexico: Mexico City"
                     /collection_date="2011-02-16"
                     /collected_by="Instituto Nacional de Cancerologia"
     gene            complement(join(9563..10554,1..118))
                     /locus_tag="Ahae2126ch_RS17580"
                     /old_locus_tag="Ahae2126ch_17760"
     CDS             complement(join(9563..10554,1..118))
                     /locus_tag="Ahae2126ch_RS17580"
                     /old_locus_tag="Ahae2126ch_17760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_004995413.1"
                     /GO_function="GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA]; GO:0051903 -
                     S-(hydroxymethyl)glutathione dehydrogenase activity
                     [Evidence IEA]"
                     /GO_process="GO:0006113 - fermentation [Evidence IEA];
                     GO:0009404 - toxin metabolic process [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase/class
                     III alcohol dehydrogenase"
                     /protein_id="WP_000842148.1"
                     /translation="MKSRAAVAFGPGQPLQIVEVDVAPPKKGEVLIKISHTGVCHTDAF
                     TLSGDDPEGVFPAILGHEGAGVVVEVGEGVTSVKPGDHVIPLYTAECGECVFCKSGKSN
                     LCISVRETQGRGVMPDGTTRFSYNGQPIYHYMGCSTFSEYTVVAEVSLAKINPEANHEH
                     VCLLGCGVTTGIGAVHNTAKVQEGDSVAVFGLGGIGLAVVQAARQAKAGRIIVVDMNPD
                     KFALAKEFGATDFLNPKDYDKPIQQVIVEMTGWGVDHSFECIGNVNVMRAALESAHRGW
                     GQSVIIGVAGAGQEISTRPFQLVTGRKWLGSAFGGVKGRTQLPGMVEDAMKGKIQLEPF
                     VTHTMGLDQINEAFDLMHEGKSIRTVIHY"
                     /name="S-(hydroxymethyl)glutathione dehydrogenase/class III
                     alcohol dehydrogenase"
     gene            complement(140..415)
                     /locus_tag="Ahae2126ch_RS17585"
                     /old_locus_tag="Ahae2126ch_17765"
     CDS             complement(140..415)
                     /locus_tag="Ahae2126ch_RS17585"
                     /old_locus_tag="Ahae2126ch_17765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001133625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal/formaldehyde-sensitive transcriptional
                     repressor"
                     /protein_id="WP_001133626.1"
                     /translation="MPNQVEDKKKILTRVRRIKGQAEAIEKALEDNVECGAILQQICSV
                     RGAINGLMNEMLEVHLKDTLVSGETTEQQRKEELAEIAKILKSYLK"
                     /name="metal/formaldehyde-sensitive transcriptional
                     repressor"
     gene            complement(729..1286)
                     /locus_tag="Ahae2126ch_RS17590"
                     /old_locus_tag="Ahae2126ch_17770"
     CDS             complement(729..1286)
                     /locus_tag="Ahae2126ch_RS17590"
                     /old_locus_tag="Ahae2126ch_17770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication DNA-binding protein"
                     /protein_id="WP_008942535.1"
                     /translation="MKTSTKYSVSDAATLYKKSRATLYKDIKNGVLSRDHDGSIDFSEL
                     LRVYGEPFSGKHSKHTDTSPTQTINTPEYTDNTVIELKNQIDFLKKQLEKAEDREAKAN
                     SRIDTLLTLIEMKPTQVHPTPTSDQSSVQSSPSQVEPTLNVDNDKHDGLTTPENKRIPV
                     PEHVEPEPKKRGFWSRFFLPYG"
                     /name="plasmid replication DNA-binding protein"
     gene            complement(1350..2231)
                     /gene="repM"
                     /locus_tag="Ahae2126ch_RS17595"
                     /old_locus_tag="Ahae2126ch_17775"
     CDS             complement(1350..2231)
                     /gene="repM"
                     /locus_tag="Ahae2126ch_RS17595"
                     /old_locus_tag="Ahae2126ch_17775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004734502.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepM"
                     /protein_id="WP_005804946.1"
                     /translation="MNKNHVVKSNQVIEASYQLSAVEQRIVLAAISRIPKNQPITDDEL
                     YPVSVDELKQLGVHEKTAYRDLKEGINRLYERSINLSIDDKSIKMRWVQEIQFLDSQSV
                     IGIRFSKPILPFISNLSREFTKYALSDIAGINSGYGIRIYELLVQYRQIGKREISVESL
                     RTMLELGKKYPLFADFKKRVIDTAIDQINEYSPLKVTYEQKKTGRKVTHIIFSFKEKSK
                     SITHTSKDFYQLSDAQINLFGNQLSRLHELSHLASEGESYEILAAKIKDMLRNPHQQQQ
                     FLPHLKNLGFKA"
                     /name="repM"
     gene            complement(2779..3948)
                     /locus_tag="Ahae2126ch_RS17600"
                     /old_locus_tag="Ahae2126ch_17780"
     CDS             complement(2779..3948)
                     /locus_tag="Ahae2126ch_RS17600"
                     /old_locus_tag="Ahae2126ch_17780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002011489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_002134206.1"
                     /translation="MASYHFSVKSKNKGYATGHYLYISRLMQYEGIRKTSNETIEHIEP
                     GRCMPAFVKDPIDFWQAADTYERANAKAYMEYEIALPNEFTSEQRKTLIETFLDKHIVP
                     QQYPHSYAIHNVKSRISGEDQPHCHLMFSLKADDGIERSAEQYFKRYNPQDPAKGGAKK
                     IQLQDGHEDYSTFLIYIRKQWENHLNDALAQHCPTVTYTLDGQDITIKNQVSADSYEKY
                     NEIHSTLYLPEPKLGVGQQNATAEYLAQIQKIREHNQQERELEERQKQLDQQHEYFYSA
                     ENNPFSSLEVMYYLNYLIKNATNKDKLKNDLNEIIKNQGPILKQEAWSIYEMNEKLEDS
                     RRNSTPCFEFEVHSLPEYVLPIQNTPPKRTQSEPVQPKADDYDYGGPGF"
                     /name="MobA/MobL family protein"
     gene            4250..5011
                     /locus_tag="Ahae2126ch_RS17605"
                     /old_locus_tag="Ahae2126ch_17785"
     CDS             4250..5011
                     /locus_tag="Ahae2126ch_RS17605"
                     /old_locus_tag="Ahae2126ch_17785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017393773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000425105.1"
                     /translation="MEMEKAKKRGRPAQLLQIAELHAFVEFLEQQEQLSDLQSQVLKAL
                     NSVDCNFEGLTQTDQVLVKEALKPYREHLKLKLLFEELNNLPLKTEYEQKFLDLYELFQ
                     KNALDQMELNILKTLATRYLNFKAQKLEYSDLELYLSQLQKKDAGKKRKAENQRKFELG
                     GAVLVAFKKLNIDISNDTPQQITNRIVNTTKFHNEVRKSLIFKDVKTYENEYFKANKLF
                     IQVLEGLHTWQKGGELLSVIEIKKALEKGEE"
                     /name="hypothetical protein"
     gene            complement(5303..5548)
                     /locus_tag="Ahae2126ch_RS17610"
                     /old_locus_tag="Ahae2126ch_17790"
     CDS             complement(5303..5548)
                     /locus_tag="Ahae2126ch_RS17610"
                     /old_locus_tag="Ahae2126ch_17790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005249014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004282220.1"
                     /translation="MYSYPDSNTEKKIALMIINDFFIQKAHELWIFLQLDQCFNDYEAT
                     LIWTRRYLEKHPECEYSDIQKAFVSCFPEHFFSFDC"
                     /name="hypothetical protein"
     gene            5698..5922
                     /locus_tag="Ahae2126ch_RS17615"
                     /old_locus_tag="Ahae2126ch_17795"
     CDS             5698..5922
                     /locus_tag="Ahae2126ch_RS17615"
                     /old_locus_tag="Ahae2126ch_17795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004676219.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_004676219.1"
                     /translation="MPYEEFQRLMGKAGLSIKEFATLLDMNPNSITNYKKIGKVPTHIA
                     VLVYLLSSMKDEGVDFYPIFEKIKSYSKD"
                     /name="helix-turn-helix transcriptional regulator"
     gene            complement(6162..6479)
                     /locus_tag="Ahae2126ch_RS17620"
                     /old_locus_tag="Ahae2126ch_17800"
     CDS             complement(6162..6479)
                     /locus_tag="Ahae2126ch_RS17620"
                     /old_locus_tag="Ahae2126ch_17800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005065053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004728121.1"
                     /translation="MLGQHHHFLKKLSISDAAIAYRKPRSTILKYIKSGDFELDYNNLI
                     EYSQLVKKLGEITSPIRDERDQQIEKLKDEVMHLKKQLNFFKYTSISNKHRLLISHKDS
                     I"
                     /name="hypothetical protein"
     gene            complement(6706..7020)
                     /locus_tag="Ahae2126ch_RS17625"
                     /old_locus_tag="Ahae2126ch_17805"
     CDS             complement(6706..7020)
                     /locus_tag="Ahae2126ch_RS17625"
                     /old_locus_tag="Ahae2126ch_17805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006582675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="WP_151800611.1"
                     /translation="MSMVRYSRKELNEKFSDKQDAEIQRLLAKGTVADDQLDLSDIPEI
                     TDWSNAVRHNQFYRPVKQQTSIRLDADVLAWFKSQGKGYQTRMNEILRDAMLKELKNHQ
                     "
                     /name="BrnA antitoxin family protein"
     gene            complement(7007..7294)
                     /locus_tag="Ahae2126ch_RS17630"
                     /old_locus_tag="Ahae2126ch_17810"
     CDS             complement(7007..7294)
                     /locus_tag="Ahae2126ch_RS17630"
                     /old_locus_tag="Ahae2126ch_17810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019383542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="WP_151800610.1"
                     /translation="MEQYFEWDEAKNRKNQKKHDVSFETASLVFDDPLRISMQDRHTDG
                     EERWQTIGKVKGVLMLLVAHTIFDEDDCEIIRIISARQVTKAERDRYEHG"
                     /name="BrnT family toxin"
     gene            complement(7805..8638)
                     /gene="fghA"
                     /locus_tag="Ahae2126ch_RS17635"
                     /old_locus_tag="Ahae2126ch_17815"
     CDS             complement(7805..8638)
                     /gene="fghA"
                     /locus_tag="Ahae2126ch_RS17635"
                     /old_locus_tag="Ahae2126ch_17815"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000439570.1"
                     /GO_function="GO:0018738 - S-formylglutathione hydrolase
                     activity [Evidence IEA]"
                     /GO_process="GO:0046292 - formaldehyde metabolic process
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="WP_000439570.1"
                     /translation="MERIEHHASFEGWQDVFQHESTVLGCMMRFGIYLPPQAEHEKLPV
                     LYWLSGLTCTEQNFITKAAVQRYAAEHGIIVVAPDTSPRGESVADDAAYDLGQGAGFYV
                     DATELPWSDHYQMYSYVVDELPALIEANFPASSRRSISGHSMGGHGALVIALRNPGRYA
                     SVSAFSPIVAPTQVPWGQKALAAYLGEKNLEAWKQYDTVELIRVAQERLPLLVDQGLSD
                     EFLEHQLRPQLLRTACEQVDHPLTLNLRPGHDHSYYFISTYLSEHMAHHAAALNR"
                     /name="fghA"
     gene            complement(9112..9510)
                     /locus_tag="Ahae2126ch_RS17640"
                     /old_locus_tag="Ahae2126ch_17825"
     CDS             complement(9112..9510)
                     /locus_tag="Ahae2126ch_RS17640"
                     /old_locus_tag="Ahae2126ch_17825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009639360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="WP_000235176.1"
                     /translation="MATPVISGNDIFSHVFLGAVDLKKSVVFYDAALGALGIKNLGPFG
                     NDWVLYGRDKPAFMITRPSNGAEPTSNGVTVGFAAATPAEVNAFHAAGLAAGGTDEGQP
                     GPRGHLPGAYAAYLRDPAGNKVCAYTFV"
                     /name="VOC family protein"
     Typing          complement(1353..2231)
                     /name="rep_cluster_1364"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="85.81"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(2783..3870)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 acacaccagt atgggagatc ttgattaata cttcaccttt cttcggtggt gcaacatcaa
       61 cctcaacaat ttgaagaggt tgaccaggac caaacgcaac agcagcacgt gacttcatgg
      121 aataattcct tatacagatc tattttaaat atgattttag aatcttggcg atttcagcca
      181 attcttcttt tcgttgttgt tctgtggttt cacctgatac taaagtgtcc tttaaatgaa
      241 cctccaacat ttcattcatt aaaccattaa tagcaccacg cacagagcat atttgttgca
      301 gaattgcacc acattccaca ttgtcttcta aagctttttc aatcgcttca gcctgaccct
      361 tgattcgtct gacacgcgtc agaatctttt ttttatcttc gacttgattt ggcataaagc
      421 taattcctta aattttatta tagtatagta cttatagtat agtaccctat agtatataga
      481 atgacaagaa gcctttataa aattaataat tgataaaagc ttataattaa aaaattctat
      541 atctattttt aagtttttat aaaattaaca taatacacct tatacgaaat gcgttataat
      601 tatctataga aatcaatgct gtagtttcta gtttatagcc catcttcccc aaggtgggtt
      661 ttttttatga tttttcacgt tccacgctta tttattgagc aatgttccac ggttttattt
      721 agcctaagtt aaccatatgg aagaaaaaaa cgactccaga agccacgctt tttaggctct
      781 ggttcaacat gctctggaac aggaatgcgc ttattctctg gagtagtcaa tccgtcatgt
      841 ttgtcgttat ctacattcaa tgtaggttct acttgagatg gactagattg cactgaagat
      901 tgatctgaag ttggtgtggg atgaacctga gttggcttca tttcaattag ggtaagtaat
      961 gtatcaatac gagaatttgc tttagcttcg cgatcttctg ctttttctaa ctgtttcttt
     1021 agaaaatcga tctgattctt tagttcaata actgtattgt ctgtgtattc aggtgtattt
     1081 atagtttgtg ttggagacgt atcagtgtgt tttgagtgtt ttccactaaa aggttcacca
     1141 taaacgcgga gtaactcaga gaagtcgata gagccatcat gatcacgact taacacccca
     1201 ttttttatat ctttataaag tgtagcacga gatttcttat acagtgtggc tgcatcacta
     1261 acactgtatt ttgtacttgt tttcatagtg tattgctgtg tatttaggat acgtattgaa
     1321 gaatacaata agtgtattct tcaatacact caagctttaa accctaagtt ctttaagtgt
     1381 ggcaaaaact gctgttgttg gtgagggttt ctaagcatat ccttaatttt agcagctaaa
     1441 atctcataac tttcaccttc tgaggctaaa tgggaaagct catgtaaacg tgataattga
     1501 ttcccgaata gattaatttg agcatcagat agctgataaa aatctttcga ggtatgtgtt
     1561 atggatttag atttttcttt aaaagaaaag ataatatgag tgactttacg ccctgttttt
     1621 ttctgctcat aagtgacttt taaagggcta tattcattga tttggtctat agcagtgtca
     1681 attactcgtt tcttgaaatc agcgaataat gggtactttt taccgagttc taacattgtc
     1741 cgtaaactct caacagaaat ttcacgctta cctatttgcc gatattgaac cagtagctca
     1801 taaatacgaa taccgtatcc actattgatc ccagcaatgt ctgataaggc atatttggtg
     1861 aattctctac ttagattgga aataaatggc agaatgggtt ttgaaaaacg gattccaatg
     1921 acactttgac tatctaaaaa ttggatttct tgcacccatc tcatttttat agatttatca
     1981 tcaatactga ggttaataga cctctcatag agtctattaa taccttcctt taaatctctg
     2041 tatgcagttt tctcatgtac gcccaattgt tttaattcat cgacgctaac agggtataac
     2101 tcatcatctg taataggctg atttttggga atccgtgaaa tggcagctaa gacaattcgt
     2161 tgctctacgg cacttaattg atacgatgct tctattactt gatttgattt tacaacgtga
     2221 tttttattca aaacaaaacc attgattttt ctaatatatg acaaatatat acttgtcata
     2281 gttagtagtc aatccgtcat agttaagtag tcaatccgtc atagttagta gtcaatccgt
     2341 catagttagt agtcaatccg tcatatttaa acgctgaaac ctttgctata taaggcacag
     2401 agacgactga aaagatttaa aagatttaaa atatattaaa aaggaaagaa tgaaaaattg
     2461 ccctatgttt tcgctacgct caaactctat tgaacttcgc ttacgctcag ttcgaagggg
     2521 caatttttgg gttaatctcg tgataacttc aaaaaagcaa aagcaactcg gaattcgctt
     2581 cgctcataag ttttttatac tcgcttcgct cgacctagat gcaaattaaa tctgttcgca
     2641 ccctccggga tgctctgtat ctcagtctca gcagcaaagg ggagtttgta atcagggaaa
     2701 aagcatggca aaaattttaa ttgtgtctgt tcgctcgtag acactcgctc tgtaaacttt
     2761 ggataaaacc aactgagctt aaaaaccagg gccaccgtaa tcataatcat cagctttagg
     2821 ttgtactggt tctgattgag tgcgtttggg aggcgtattt tgtatcggta atacgtattc
     2881 aggaagtgaa tgcacctcga actcaaaaca tggggtactg ttacgtcttg agtcttctag
     2941 tttttcattc atttcgtaga tggaccaagc ttcttgttta agtatagggc cttggttttt
     3001 gataatttca ttcaaatcat tttttagttt gtccttattt gttgcattct tgattaagta
     3061 attcaggtaa tacatgactt ccaatgatga aaatgggttg ttttcagcac tataaaaata
     3121 ttcatgttgt tgatctaatt gcttttggcg ttcttcgagt tctcgttctt gctggttgtg
     3181 ttctctgatt ttttggattt gggcgagata ttccgctgtc gcattttgtt gtccaacgcc
     3241 tagttttggc tctggaaggt atagcgtgct gtggatctcg ttatattttt catagctgtc
     3301 tgctgaaacc tgatttttga tggtgatgtc ttggccatcc agtgtgtagg tgacggtcgg
     3361 gcagtgctgt gcaagggcat cattcaaatg attttcccat tgttttcgga tatagatcaa
     3421 aaaagtggaa tagtcttcat ggccatcttg aagttggatt tttttggcac cgcctttggc
     3481 tggatcttga gggttatagc gtttgaagta ctgttctgcg ctgcgttcaa tgccgtcatc
     3541 cgcttttagc gagaacatca gatgacaatg aggctgatct tcgccactga tacgtgattt
     3601 tacgttgtgt atggcgtatg agtgcggata ttgctgtgga actatatgtt tgtctaaaaa
     3661 cgtctctatg agcgttttac gctgttctga cgtgaactca ttcggtaggg caatttcata
     3721 ctccatgtag gccttggcat tggctcgttc atacgtatct gctgcctgcc aaaaatcaat
     3781 gggatctttt acaaaggcag gcatacatcg accaggttca atatgttcta tggtctcatt
     3841 gctggttttg cggatgcctt catactgcat cagccgggaa atgtagagat aatggccggt
     3901 cgcatagccc ttatttttgc ttttgacgct gaagtggtag ctggccatgt tcacctctgc
     3961 tgggttgtgg gttcggtttt tatgcagtga aacggaatga aaaccgcccg gatttgaaat
     4021 gcctgtatgg ctgaaaaatc cggttgaagg gggtcaaggg ggaagcgccc cattgcgtac
     4081 tagtttctcc gatgaggaga aactgtcaag tgcgaattat ttaatataaa aataaaaatt
     4141 cttttcaaga aattttattt ttatattaaa taattatttt cagactgccc tctttaaata
     4201 tggtaaaacc atatttaaag agggcagtct gaaaatttgg cggcagatga tggaaatgga
     4261 aaaagcaaaa aagcgtggtc gacctgctca gttgttacag attgctgagt tacatgcatt
     4321 tgtagagttt ttagaacagc aagaacagct ttctgatctt caatctcaag tgttaaaggc
     4381 tttaaatagt gttgattgta attttgaggg gctaactcag acagatcagg ttctggtcaa
     4441 agaggcttta aaaccttatc gtgaacatct gaagttgaaa ttgctgtttg aagagttgaa
     4501 taatctgcct cttaaaaccg agtatgaaca gaagttttta gatctgtatg aactttttca
     4561 aaagaatgca ctagatcaga tggaactaaa tattttaaaa accttggcga cacggtatct
     4621 gaattttaaa gcgcaaaagc ttgaatacag tgatttagaa ttgtatttga gtcagctaca
     4681 gaagaaggat gctgggaaaa aacgcaaagc tgaaaatcaa cgtaaatttg aattaggtgg
     4741 tgctgttctt gtcgcattta agaaactcaa tattgatatt agcaatgata ctcctcaaca
     4801 gattaccaat cggattgtga atacgacaaa gtttcataat gaagtacgta aatctttaat
     4861 ttttaaagat gttaagacat atgaaaatga atattttaaa gcaaataaac tttttattca
     4921 ggtgttagaa ggattacata cctggcaaaa gggcggagaa ttattgtcag taattgaaat
     4981 taaaaaggcc ttagaaaaag gcgaagagta gtttatttag cagggatttt agagaagttg
     5041 gacatttcta ttttgggctt acaatcgaat ttcgtataca gagacattat gtaaatttag
     5101 agtacaccct aaagtaacgt catggcgacc ccaatcctga acctaaaatc aatgttttag
     5161 aagccgtgga acatcaattt tagtatgctc aaagaaagtc ttagatttat acgtgacaat
     5221 agaaagagcc agttgtatga actggctctt tctatttggg atttacttac ccattgcgat
     5281 tttttaccgt gcctctcata aattaacagt caaagctgaa gaagtgctct gggaaacaag
     5341 aaacaaaggc tttttgaata tcgctgtact cacactcagg atgcttctct aaataacgtc
     5401 gagtccaaat cagtgtggct tcataatcat taaagcactg atcgagttgt aaaaagatcc
     5461 acagttcatg ggctttttga atgaaaaagt cattgatgat catgagagca attttctttt
     5521 cagtgtttga atccgggtat gagtacatat tacatttctc cgatagtaag gataaatagg
     5581 ttagttgata agctatttat aacacataaa aaatgtgttt gtcacatttt ttatgtgttt
     5641 ttttaagttt tatagtctgt gttatacttc taattcaaac agattgtatt tatcacgatg
     5701 ccatacgaag aatttcaaag acttatgggg aaagcaggtc tttcaattaa ggaatttgca
     5761 accttattgg acatgaatcc aaactccata actaactata aaaagatcgg aaaagtacct
     5821 actcatatcg ctgttctggt ttacttactt tctagcatga aagatgaggg tgtagacttt
     5881 taccctattt ttgaaaaaat taagtcgtat tcaaaagact aaaactctcg tttgttgatt
     5941 gttgtaagct gttagtgatg acgtaaaaaa tgcctgaagt tcaggcattt ttttgtttca
     6001 gggacttata agcctcgata gaacttttca ataaagtatc gagttttgag aaccagggtg
     6061 atcatcaaat tcttatgttt tgagatctta ggtcgagcaa tcggtaaacc ggacatttct
     6121 atttggggat tacaactgaa tttcgcataa gctctattat gttaaatgga gtctttatga
     6181 gaaattaata atctatgttt gtttgagatt gaagtatatt taaaaaaatt aagttgtttt
     6241 tttaaatgca taacttcatc ttttagtttt tcgatttgtt gatctctttc gtcccgtata
     6301 ggggaagtaa tttcacccaa ttttttaact aactggctat attcaattaa gttattgtag
     6361 tcaagttcga aatctccgct ttttatatat ttaagtatag tcgagcgagg ttttctatac
     6421 gctatagcag catcactgat cgaaagtttt tttaaaaaat gatgatgttg acctaacata
     6481 ttatttaact taagacatta ttaatttgat agatatatat taataaaatt aacaaatgta
     6541 agatataaat cttcaatcat tattggttga taattgaacg tttactttac acatacccta
     6601 ctctgataat ttttctatta ttaaatcaat acgcttttaa gaatttatat tattaacata
     6661 atacacgtta tgcgaagtca aaaatgggag cttaagctcc catttttatt gatgattttt
     6721 taattctttc agcatagcat ctcgcagaat ttcattcatt cgtgtttgat aacctttgcc
     6781 ttgagattta aaccatgcaa gtacatcagc atctaaacga atggaagtct gttgcttcac
     6841 tggacggtag aactgattat ggcgtacagc attgctccag tcggtaattt caggaatatc
     6901 tgatagatct agctgatcgt cagcaaccgt tcccttagca agcaagcgtt gaatttcagc
     6961 atcttgcttg tcgctgaatt tttcattcag ttctttgcgt gagtatctaa ccatgctcat
     7021 atctatctcg ctccgcctta gtgacttgtc ttgcactaat gattcgtatg atttcacagt
     7081 catcttcatc aaagatggtg tgagctacta agagcattag tacgcctttt acttttccaa
     7141 tggtctgcca acgttcttca ccatcggtat gtctatcctg cattgagatc ctcagtggat
     7201 catcaaaaac aaggcttgcc gtttcgaaag agacatcgtg ttttttctga ttctttcgat
     7261 tctttgcctc atcccattcg aaatactgtt ccataaaaaa cattcaaatt tgtatataca
     7321 ataatgtatc acaaaattgt atcactcaag ttgaaaagcg taatttttag tcaaatttat
     7381 tggcaaaaca ttccaaaact gctcacaaaa aagccgactt gtttcaaaat cggctttttg
     7441 ggcatttttg gatttttata tttttattaa gtagttgata tttaaattaa tatacaagtg
     7501 tattgcgtat aatcgccatt atgttaaatg gatagttaac tacatggtat ttctgttcga
     7561 ttaactagct ctttcagctt ttccaagaaa tcacggctta gtattagctc ctctactttt
     7621 ttatctgata tgggctttgt tgcataaaga ttttgtaagc ttctgattta aataggtttg
     7681 tatgaatttc aacctaaaaa ttaaatgaac aaaatcagtt ggttatgtat ttatgcaaca
     7741 aagcccctta aaaccaaata gaaatgtcct atacagacat ttccaagtca gggtctccca
     7801 atgttcagcg attcaatgca gccgcatggt gggccatgtg ttcgctcaag taggtcgaaa
     7861 taaagtagta gctatggtca tgaccaggcc gcagattgag cgttagcggg tggtctacct
     7921 gttcacacgc ggtacgcaac agttgcggtc gaagctggtg ctcgagaaac tcatcgctca
     7981 gcccttgatc gacaagcagc ggtaagcgtt cttgcgccac gcgaatcagc tcgaccgtgt
     8041 catattgctt ccaggcttcc agattctttt cgcccaagta agccgccaga gccttctgcc
     8101 cccatggaac ttgcgtaggc gccacgatcg gcgagaacgc cgagacactt gcatagcgac
     8161 cggggttgcg aagcgcaata accagagcgc catgaccgcc catggagtgc ccactgatgc
     8221 tgcgtcttga cgacgcaggg aagttcgctt cgatcagcgc tggtaattcg tcgaccacat
     8281 aggaatacat ctgatagtga tcgctccaag gtagctctgt ggcgtctaca tagaaacccg
     8341 caccttgacc tagatcatag gcagcatcgt ctgcaacgct ttctccgcgc gggctggtat
     8401 ctggggctac gacaatgatt ccgtgctccg cggcataccg ctgcaccgca gccttggtga
     8461 tgaagttctg ttcggtacag gtcagacctg agagccagta cagaactggg agcttttcat
     8521 gctcagcctg aggcggaaga tagattccaa acctcatcat gcagccaagc actgttgact
     8581 catgctgaaa aacgtcctgc caaccctcga agctggcgtg atgttcaatg cgttccacgg
     8641 attttctcct tatttcaacg aggtggtgct caggcgccaa tcaagacgaa ttttcgttcc
     8701 atccaggatt agttaaaaat gcttcaccgc accactcagc aggttttcag cgttgtcgct
     8761 ggcatgaaga tcgctgctac aaacccagac gccaccacac cacgaaggtc ggatgtcgac
     8821 ttcaatgtac aaacaataaa gtgtgtaatt cagggagcca ctaccgcgat gctcattttg
     8881 gattctgtgt ttcttgcatc acgcaccgaa ctttcagtcc gatcgtgctc attgaccgtc
     8941 tttggctatg aaggcgaggt aacagccttt ctgagctcta atcagcagat agaggctcag
     9001 agtaccgtaa accgtgtggc atgagcgggc atgccacacg gttgcagtgg aagatgagat
     9061 tgtttttggc tggaaccagt ccttacacaa tgacctcact tctcctaggt tttagacaaa
     9121 ggtgtaggcg cagaccttat taccagccgg atcacgcagg taggccgcgt aagcacccgg
     9181 caggtggcca cgtgggccag gctgaccttc atcggtgcca ccagcggcga ggccggctgc
     9241 gtggaatgca tttacctctg caggggtggc agctgcgaag cctaccgtca caccattact
     9301 ggtgggttct gcgccattgc ttgggcgggt gatcatgaaa gcaggtttgt cgcgaccgta
     9361 cagaacccaa tcgttaccga atggccctag gtttttaatg ccgagggcac ctagagcggc
     9421 atcgtagaat acaaccgact tcttaaggtc gactgcgccg aggaagacat gggagaaaat
     9481 atcgttaccg gaaattactg gagtagccat taaagtgttt ccttgatggt tggttttgaa
     9541 aaattaggta ttgatccgtg gatcaatagt gaatgacagt gcgaattgac ttaccttcat
     9601 gcattagatc aaaggcttca ttgatctgat ccaagcccat cgtatgtgtc acaaatggtt
     9661 ctaattgaat tttacctttc atggcatctt caaccattcc tggaagttgc gtacgacctt
     9721 ttacgccacc aaaggcagaa cctagccatt tacgaccagt aaccagttgg aatggacggg
     9781 tagagatttc ctgacctgcg ccagctacac caataattac ggattgtccc cagccacggt
     9841 gtgcactttc aagggctgca cgcattacat tcacgttgcc aatacattca aatgaatgat
     9901 ctacacccca gccagtcatt tcgacaatga cttgctggat tggtttatcg tagtctttag
     9961 ggttcaagaa gtccgttgca ccaaactctt ttgccagcgc aaatttatct ggattcatgt
    10021 caaccacaat aatacgccct gctttagctt gacgtgcggc ttgtacgact gccaaaccaa
    10081 taccacctaa accaaagacc gcaacactat caccttcttg tactttagcc gtgttatgta
    10141 ctgcaccgat accggttgtt accccacaac ccagtaagca gacatgttcg tgattggctt
    10201 caggattgat tttagccaat gatacttctg ccactacagt gtattcactg aacgttgagc
    10261 agcccatata gtggtaaatc ggttgaccat tgtaggaaaa gcgagtggta ccatctggca
    10321 ttacgcctct accttgagtt tcgcgaacgg aaatacacaa gttcgatttt ccagatttac
    10381 agaatacgca ctcgccacat tcagcagtgt aaagtggaat aacatgatca cctggtttga
    10441 cgctagtcac gccttcacca acctcaacta caacaccagc accttcatgc ccgagaatag
    10501 ccggaaaaac gccttcagga tcatcgccag ataaagtaaa tgcgtctgta tgac
//
