LOCUS       NZ_CP031983             4281 bp    DNA     circular CON 05-JAN-2024
DEFINITION  Acinetobacter haemolyticus strain AN4 plasmid pAhaemAN4a, complete
            sequence.
ACCESSION   NZ_CP031983
VERSION     NZ_CP031983.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN09927973
            Assembly: GCF_009892285.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter haemolyticus
  ORGANISM  Acinetobacter haemolyticus
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter.
REFERENCE   1  (bases 1 to 4281)
  AUTHORS   Castro-Jaimes,S. and Cevallos,M.A.
  TITLE     Analysis of the genomic diversity of Mexican Acinetobacter
            haemolyticus clinical isolates
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4281)
  AUTHORS   Castro-Jaimes,S. and Cevallos,M.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-AUG-2018) Evolutionary Genomics Program, Center for
            Genomic Sciences, National Autonomous University of Mexico (UNAM),
            Av. Universidad s/n Col. Chamilpa, Cuernavaca, Morelos 62210, Mexico
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: AUG-2018
            Assembly Method                   :: Unicycler v. 0.4.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 104x
            Sequencing Technology :: Illumina MiSeq and PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/05/2024 04:35:17
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,612
            CDSs (total)                      :: 3,518
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 94
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 34 of 165
            Pseudo Genes (incomplete)         :: 135 of 165
            Pseudo Genes (internal stop)      :: 22 of 165
            Pseudo Genes (multiple problems)  :: 23 of 165
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP031983.1.
            Bacteria available from Submitting center.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4281
                     /organism="Acinetobacter haemolyticus"
                     /mol_type="genomic DNA"
                     /strain="AN4"
                     /isolation_source="Ulcer by decubitus"
                     /host="Homo sapiens"
                     /db_xref="taxon:29430"
                     /plasmid="pAhaemAN4a"
                     /geo_loc_name="Mexico: Puebla"
                     /collection_date="2010-11-26"
                     /collected_by="Hospital para el Nino Poblano"
     gene            complement(join(4142..4281,1..163))
                     /locus_tag="AhaeAN4_RS17940"
                     /old_locus_tag="AhaeAN4_18070"
     CDS             complement(join(4142..4281,1..163))
                     /locus_tag="AhaeAN4_RS17940"
                     /old_locus_tag="AhaeAN4_18070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005320422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="WP_160127448.1"
                     /translation="MTDEKEPKTRLTLEQKIQLQEQKLAQLKAQQEAKLAKEKERQKEQ
                     DRKDDTRIKILLGSYLKKKMDSNPDFNSQILDELENYLTAERDRKLFGLSPLDQG"
                     /name="mobilization protein"
     gene            387..1967
                     /gene="mobQ"
                     /locus_tag="AhaeAN4_RS17945"
                     /old_locus_tag="AhaeAN4_18075"
     CDS             387..1967
                     /gene="mobQ"
                     /locus_tag="AhaeAN4_RS17945"
                     /old_locus_tag="AhaeAN4_18075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005000602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_160132300.1"
                     /translation="MAIYHFSVKAISRGAGRSAVACAAYRSGEKLVCDYYGIEQDYTKK
                     TGVEFTNIYAPENTNPNLLDRQKLWNAVEKSERRKDALLAREFEIAFPKELNQEQRQTL
                     LDELCQGIVKKHGVVVDAAIHAPHTGSGSDERNYHAHVMFTTRAINKNGTFEAKKYRDF
                     SRDNGTETVSNWRENFADLTNRHLKQAGYDVQVDHRSYADQDNGLEATEHEGPAVTQLR
                     RMGIATEISSENDAIKQRNAEKTVNEQAITGLEQEIIASEAILSDLQQEKAKSDAEHAQ
                     QEKIAAERKKERMTAQAIELAHDRATAFHAAFNPDQPNKQLVMQYIQDHALLSRYGKEP
                     QPPAPVEQLQPTWWQRMTGQQPQQIEQPPFFEIEVIKATAQAIVEREQEQAQQQKWERL
                     QEFLQHSPSDVDRIERDAIGRSFSKGIDAKDEELSEFIKYHRQHWREKEQAAKDWQAEY
                     DQFMREEAAKANAEAEAKLQRERESEARLEAYRESKAQVDKEISEMSKKPVNNRDNEPK
                     PRQKNDFDF"
                     /name="mobQ"
     gene            2636..3640
                     /gene="repM"
                     /locus_tag="AhaeAN4_RS17950"
                     /old_locus_tag="AhaeAN4_18080"
     CDS             2636..3640
                     /gene="repM"
                     /locus_tag="AhaeAN4_RS17950"
                     /old_locus_tag="AhaeAN4_18080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005404019.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepM"
                     /protein_id="WP_216071035.1"
                     /translation="MLVVKDNALINASYNLELVEQRLILLAIIEARETGKGINANDALT
                     IHASSYIQNFNVQKHTAYGALKDACRDLFSRQFSYQELSPKGNVINQTSRWVSQIGYIQ
                     EEGSVQLIFAPAVVPLITQLEQQFTSYDLEQVSTLKSKYATRLYEILISWRSVGKTPMI
                     GLEELRGKLGLGVNDYKLLANFKLRVLELALEQINEHTDIFVKYEQHKKGRSIIGFSFK
                     FKQKQQSKIEKPIDQKRDPKTVDMFTGKTDAETKNIARAVVKDTSPPANPLLHRASRIT
                     TSIITHKLHERFKHGDEGLSSTLKRIQGEIMEGGEETADYWQSKLEDFGITFD"
                     /name="repM"
     gene            3704..3892
                     /locus_tag="AhaeAN4_RS17955"
                     /old_locus_tag="AhaeAN4_18085"
     CDS             3704..3892
                     /locus_tag="AhaeAN4_RS17955"
                     /old_locus_tag="AhaeAN4_18085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004676352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_005404020.1"
                     /translation="MNTRFDPIVSEFESSDHEAEYNAWFIAKVEKSLADSRPTIPHDQV
                     VARMKKRREEREANANR"
                     /name="hypothetical protein"
     gene            3879..4151
                     /locus_tag="AhaeAN4_RS17960"
                     /old_locus_tag="AhaeAN4_18090"
     CDS             3879..4151
                     /locus_tag="AhaeAN4_RS17960"
                     /old_locus_tag="AhaeAN4_18090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004676351.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_004676351.1"
                     /translation="MLIVKWQPTAVEDLEAIIDYIANHNTVAAYDLEDHILQSAEDLAE
                     HPYKGRNGRRAGTRELLVHPNYWLVYEVSDLIRITNVIHTSRLYP"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
ORIGIN
        1 tctttatgcg tgtgtcatct ttccgatctt gctctttctg acgttctttc tcttttgcca
       61 gttttgcttc ctgctgtgct tttaactgtg ctagtttttg ctcttgtagt tgtatttttt
      121 gttcaagtgt taatcttgtc tttggctctt tttcgtcagt catgcttttt ccatatatca
      181 aggattcctc tcgatcataa tcctgtttga actggtgttc aagacagtat atgatactcg
      241 aagaatcccg gaagggcgca cttagccatt aatccataaa cttcgtatgg gttaatggtg
      301 cgaaagggga aatccccctt taccccctga caaatttcgt tcctcaattt gtatttcgct
      361 aaagcgaaat aagagcaaaa gcaaatatgg caatttatca tttttcagtg aaagcgatta
      421 gcagaggtgc aggacgtagt gccgttgctt gtgctgccta tcgttcaggt gaaaaattgg
      481 tatgtgacta ttatggaata gagcaagact acacaaaaaa aacaggtgtt gaatttacaa
      541 atatttatgc accagagaat acaaatccca atttgttaga tcgtcaaaaa ttgtggaatg
      601 ctgtagaaaa atcagagcgc agaaaagatg ctttacttgc ccgtgaattt gaaattgcct
      661 ttcccaaaga attaaaccag gaacaacgcc aaaccctact cgatgagtta tgccaaggca
      721 ttgtaaaaaa acatggtgtt gttgttgatg cagcaattca cgcaccgcat acaggtagtg
      781 gatctgacga acgtaactac cacgcccatg tcatgtttac gaccagagct attaataaaa
      841 atggaacgtt tgaagctaaa aaatatcgtg acttcagtcg tgacaatggg acagaaacgg
      901 tcagcaactg gagagaaaat tttgcagact tgaccaaccg acatcttaag caggcaggct
      961 atgacgttca ggttgatcat cgtagttatg ctgaccaaga caacggactt gaggccacag
     1021 aacacgaagg cccagccgta acacaactta ggcgcatggg gatagctacc gaaatcagca
     1081 gtgaaaatga tgcaatcaaa cagcgtaacg ctgaaaaaac tgtaaatgag caggcgatca
     1141 ctggattaga gcaagaaatc atagcgtcag aggcgattct aagcgattta cagcaagaaa
     1201 aagctaaaag cgatgcagaa catgcacagc aagaaaaaat tgcagcagaa cgcaaaaaag
     1261 agcgaatgac agcacaagct atcgagcttg cacatgatag agctacagcc tttcatgcag
     1321 cgtttaatcc tgatcaaccc aacaaacagc ttgtcatgca gtacatacag gatcatgcct
     1381 tgcttagtcg ttacggcaaa gagccacagc cccctgcccc agtagaacaa ctacagccaa
     1441 cttggtggca acgcatgaca gggcaacagc cacaacaaat tgaacaaccg ccattttttg
     1501 aaatcgaggt gatcaaagcc accgcccaag ctattgttga gcgtgaacaa gagcaagcgc
     1561 aacagcagaa atgggagcgg ttacaagagt ttttacagca ctctcctagc gatgtagacc
     1621 gaattgaaag ggacgccatc ggcagatcct tcagtaaagg catagatgcc aaagacgaag
     1681 aactttccga gttcatcaaa taccaccgcc aacattggcg ggaaaaagag caggcagcga
     1741 aagactggca agccgaatat gatcaattca tgcgagaaga agcagcgaaa gccaacgctg
     1801 aagcggaagc caagctacaa agagagcgtg agtcagaagc ccgactggaa gcgtacagag
     1861 agagcaaggc gcaggtggac aaggaaataa gtgaaatgtc caaaaagcct gttaataata
     1921 gggataatga gccaaaacca cgccaaaaaa atgactttga tttttagttt ttctaaacaa
     1981 tacatgatct tacagagcga gcgtctgcga gcgaacaacg aaatttcgcc ctttcctcct
     2041 ctgaaaaacc gctttttacg gttttcgccc ctagaaagta actattttgt gtgctgattt
     2101 tggcgtatct catcttatta acagtccata acccttgatt ttattggatt tcgtgcgctt
     2161 tttctcttaa aaaagcatcg agcgaagcga gattcatagt cgcttttgct tttcccaacg
     2221 ccctagatgg tatttgctaa aaactgcccc tcagaccgag tgaaacgagg tcaaaatgcc
     2281 aagagcgaag cgagtgccat agggcagttt ttgtagtgct tttgagcttt taatgctttt
     2341 aatgctttta aaagctttta gacgagctga aagccttatc tagtaatgat ttcagagatt
     2401 atatatatac gtttaccgac ccatatatat acgtttaccg acccatatat atacgtttac
     2461 cgacccatat atatacgttt accgaccata tatatacaaa taaattaatg ttatatattt
     2521 acataaattt atttgtatgc ctatataaca tacaaggcaa gtatatgtct cttggagtac
     2581 caacaaaatg acctcaatcc agaaagcaaa tgcaccacca cgaggaacag ccgatatgtt
     2641 ggtagtcaaa gataatgcgt taattaacgc cagttataat ttagagttag tcgaacaaag
     2701 attgatttta ttagcaatta ttgaagcaag agaaacagga aagggaatta atgccaatga
     2761 tgccttaacc atccatgcct caagttatat tcaaaatttt aatgttcaaa agcatacagc
     2821 ctatggtgcg ttgaaagatg cttgccgaga cctattttca aggcaattta gttatcaaga
     2881 gttatcaccg aaaggtaatg ttataaatca gacatcaagg tgggtgagcc aaattggtta
     2941 tattcaggag gaaggtagtg tacaactaat ctttgctcct gccgttgttc cacttattac
     3001 acagttagaa cagcagttta ccagttacga cctagagcaa gtctcaactc ttaaaagcaa
     3061 gtatgcaact cgcttgtatg aaatacttat ttcatggcgt agcgtaggca aaacaccaat
     3121 gatagggcta gaggaactga gaggaaaact aggactaggt gttaatgact ataagctttt
     3181 agcaaacttt aaattacggg tattagagct tgcacttgaa cagattaatg aacatacaga
     3241 catttttgta aaatacgagc agcataaaaa aggacgtagc attattggct tttcattcaa
     3301 gtttaaacaa aaacaacagt caaaaattga aaaaccgatt gatcagaagc gagatccaaa
     3361 gaccgttgat atgttcacag gtaaaactga tgctgagaca aaaaacatag ctagagcagt
     3421 tgttaaagac acctcaccgc ctgcgaatcc cttgttgcat agggcaagtc gtattacaac
     3481 gtcaattatc acccataaac tgcacgaacg tttcaaacat ggcgatgagg gtttatctag
     3541 tacattgaag cgtattcaag gcgaaattat ggagggaggg gaggaaacgg cagactactg
     3601 gcaatcgaaa cttgaggatt ttgggataac ttttgattaa aaaataccca tgatttttgt
     3661 accgtaaagc agtaaacttg agcgaataat tggagtacca actatgaata caaggtttga
     3721 tcctatcgtg tcagagtttg aaagttctga ccatgaagca gaatacaatg cgtggtttat
     3781 tgcaaaagtt gagaaatcac tagccgattc acgccccacc attccccatg accaagtagt
     3841 ggcacgtatg aaaaaacgtc gtgaggaaag agaagccaat gctaatcgtt aagtggcaac
     3901 caaccgcagt agaagatttg gaagcaataa ttgactatat cgccaaccat aatactgttg
     3961 ccgcatatga cctagaagac catattctac aatctgctga agatttggca gaacacccat
     4021 acaaagggcg taatggtcga agagcaggaa caagagagtt acttgtccat ccaaattatt
     4081 ggttagtcta tgaggtttca gacctgattc gcattaccaa tgtaatacat acgagtagac
     4141 tctatccctg atcaagaggg gatagtccaa acagttttct atctcgttcg gctgtcagat
     4201 agttttccag ttcatctaga atctgagaat tgaaatctgg gttactgtcc atttttttct
     4261 tcaaataaga accgagcaga a
//
