LOCUS       NZ_CP031981            10554 bp    DNA     circular CON 05-JAN-2024
DEFINITION  Acinetobacter haemolyticus strain AN4 plasmid pAhaemAN4c, complete
            sequence.
ACCESSION   NZ_CP031981
VERSION     NZ_CP031981.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN09927973
            Assembly: GCF_009892285.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter haemolyticus
  ORGANISM  Acinetobacter haemolyticus
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter.
REFERENCE   1  (bases 1 to 10554)
  AUTHORS   Castro-Jaimes,S. and Cevallos,M.A.
  TITLE     Analysis of the genomic diversity of Mexican Acinetobacter
            haemolyticus clinical isolates
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10554)
  AUTHORS   Castro-Jaimes,S. and Cevallos,M.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-AUG-2018) Evolutionary Genomics Program, Center for
            Genomic Sciences, National Autonomous University of Mexico (UNAM),
            Av. Universidad s/n Col. Chamilpa, Cuernavaca, Morelos 62210, Mexico
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: AUG-2018
            Assembly Method                   :: Unicycler v. 0.4.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 104x
            Sequencing Technology :: Illumina MiSeq and PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/05/2024 04:35:17
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,612
            CDSs (total)                      :: 3,518
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 94
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 34 of 165
            Pseudo Genes (incomplete)         :: 135 of 165
            Pseudo Genes (internal stop)      :: 22 of 165
            Pseudo Genes (multiple problems)  :: 23 of 165
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP031981.1.
            Bacteria available from Submitting center.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10554
                     /organism="Acinetobacter haemolyticus"
                     /mol_type="genomic DNA"
                     /strain="AN4"
                     /isolation_source="Ulcer by decubitus"
                     /host="Homo sapiens"
                     /db_xref="taxon:29430"
                     /plasmid="pAhaemAN4c"
                     /geo_loc_name="Mexico: Puebla"
                     /collection_date="2010-11-26"
                     /collected_by="Hospital para el Nino Poblano"
     gene            join(9724..10554,1..339)
                     /locus_tag="AhaeAN4_RS17850"
                     /old_locus_tag="AhaeAN4_17975"
     CDS             join(9724..10554,1..339)
                     /locus_tag="AhaeAN4_RS17850"
                     /old_locus_tag="AhaeAN4_17975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002011489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_002134206.1"
                     /translation="MASYHFSVKSKNKGYATGHYLYISRLMQYEGIRKTSNETIEHIEP
                     GRCMPAFVKDPIDFWQAADTYERANAKAYMEYEIALPNEFTSEQRKTLIETFLDKHIVP
                     QQYPHSYAIHNVKSRISGEDQPHCHLMFSLKADDGIERSAEQYFKRYNPQDPAKGGAKK
                     IQLQDGHEDYSTFLIYIRKQWENHLNDALAQHCPTVTYTLDGQDITIKNQVSADSYEKY
                     NEIHSTLYLPEPKLGVGQQNATAEYLAQIQKIREHNQQERELEERQKQLDQQHEYFYSA
                     ENNPFSSLEVMYYLNYLIKNATNKDKLKNDLNEIIKNQGPILKQEAWSIYEMNEKLEDS
                     RRNSTPCFEFEVHSLPEYVLPIQNTPPKRTQSEPVQPKADDYDYGGPGF"
                     /name="MobA/MobL family protein"
     gene            887..1768
                     /gene="repM"
                     /locus_tag="AhaeAN4_RS17855"
                     /old_locus_tag="AhaeAN4_17980"
     CDS             887..1768
                     /gene="repM"
                     /locus_tag="AhaeAN4_RS17855"
                     /old_locus_tag="AhaeAN4_17980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004734502.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepM"
                     /protein_id="WP_005804946.1"
                     /translation="MNKNHVVKSNQVIEASYQLSAVEQRIVLAAISRIPKNQPITDDEL
                     YPVSVDELKQLGVHEKTAYRDLKEGINRLYERSINLSIDDKSIKMRWVQEIQFLDSQSV
                     IGIRFSKPILPFISNLSREFTKYALSDIAGINSGYGIRIYELLVQYRQIGKREISVESL
                     RTMLELGKKYPLFADFKKRVIDTAIDQINEYSPLKVTYEQKKTGRKVTHIIFSFKEKSK
                     SITHTSKDFYQLSDAQINLFGNQLSRLHELSHLASEGESYEILAAKIKDMLRNPHQQQQ
                     FLPHLKNLGFKA"
                     /name="repM"
     gene            1832..2389
                     /locus_tag="AhaeAN4_RS17860"
                     /old_locus_tag="AhaeAN4_17985"
     CDS             1832..2389
                     /locus_tag="AhaeAN4_RS17860"
                     /old_locus_tag="AhaeAN4_17985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication DNA-binding protein"
                     /protein_id="WP_008942535.1"
                     /translation="MKTSTKYSVSDAATLYKKSRATLYKDIKNGVLSRDHDGSIDFSEL
                     LRVYGEPFSGKHSKHTDTSPTQTINTPEYTDNTVIELKNQIDFLKKQLEKAEDREAKAN
                     SRIDTLLTLIEMKPTQVHPTPTSDQSSVQSSPSQVEPTLNVDNDKHDGLTTPENKRIPV
                     PEHVEPEPKKRGFWSRFFLPYG"
                     /name="plasmid replication DNA-binding protein"
     gene            2703..2978
                     /locus_tag="AhaeAN4_RS17865"
                     /old_locus_tag="AhaeAN4_17990"
     CDS             2703..2978
                     /locus_tag="AhaeAN4_RS17865"
                     /old_locus_tag="AhaeAN4_17990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001133625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal/formaldehyde-sensitive transcriptional
                     repressor"
                     /protein_id="WP_001133626.1"
                     /translation="MPNQVEDKKKILTRVRRIKGQAEAIEKALEDNVECGAILQQICSV
                     RGAINGLMNEMLEVHLKDTLVSGETTEQQRKEELAEIAKILKSYLK"
                     /name="metal/formaldehyde-sensitive transcriptional
                     repressor"
     gene            3000..4109
                     /locus_tag="AhaeAN4_RS17870"
                     /old_locus_tag="AhaeAN4_17995"
     CDS             3000..4109
                     /locus_tag="AhaeAN4_RS17870"
                     /old_locus_tag="AhaeAN4_17995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_004995413.1"
                     /GO_function="GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA]; GO:0051903 -
                     S-(hydroxymethyl)glutathione dehydrogenase activity
                     [Evidence IEA]"
                     /GO_process="GO:0006113 - fermentation [Evidence IEA];
                     GO:0009404 - toxin metabolic process [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase/class
                     III alcohol dehydrogenase"
                     /protein_id="WP_000842148.1"
                     /translation="MKSRAAVAFGPGQPLQIVEVDVAPPKKGEVLIKISHTGVCHTDAF
                     TLSGDDPEGVFPAILGHEGAGVVVEVGEGVTSVKPGDHVIPLYTAECGECVFCKSGKSN
                     LCISVRETQGRGVMPDGTTRFSYNGQPIYHYMGCSTFSEYTVVAEVSLAKINPEANHEH
                     VCLLGCGVTTGIGAVHNTAKVQEGDSVAVFGLGGIGLAVVQAARQAKAGRIIVVDMNPD
                     KFALAKEFGATDFLNPKDYDKPIQQVIVEMTGWGVDHSFECIGNVNVMRAALESAHRGW
                     GQSVIIGVAGAGQEISTRPFQLVTGRKWLGSAFGGVKGRTQLPGMVEDAMKGKIQLEPF
                     VTHTMGLDQINEAFDLMHEGKSIRTVIHY"
                     /name="S-(hydroxymethyl)glutathione dehydrogenase/class III
                     alcohol dehydrogenase"
     gene            4162..4560
                     /locus_tag="AhaeAN4_RS17875"
                     /old_locus_tag="AhaeAN4_18000"
     CDS             4162..4560
                     /locus_tag="AhaeAN4_RS17875"
                     /old_locus_tag="AhaeAN4_18000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009639360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="WP_000235176.1"
                     /translation="MATPVISGNDIFSHVFLGAVDLKKSVVFYDAALGALGIKNLGPFG
                     NDWVLYGRDKPAFMITRPSNGAEPTSNGVTVGFAAATPAEVNAFHAAGLAAGGTDEGQP
                     GPRGHLPGAYAAYLRDPAGNKVCAYTFV"
                     /name="VOC family protein"
     gene            5034..5867
                     /gene="fghA"
                     /locus_tag="AhaeAN4_RS17880"
                     /old_locus_tag="AhaeAN4_18010"
     CDS             5034..5867
                     /gene="fghA"
                     /locus_tag="AhaeAN4_RS17880"
                     /old_locus_tag="AhaeAN4_18010"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000439570.1"
                     /GO_function="GO:0018738 - S-formylglutathione hydrolase
                     activity [Evidence IEA]"
                     /GO_process="GO:0046292 - formaldehyde metabolic process
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="WP_000439570.1"
                     /translation="MERIEHHASFEGWQDVFQHESTVLGCMMRFGIYLPPQAEHEKLPV
                     LYWLSGLTCTEQNFITKAAVQRYAAEHGIIVVAPDTSPRGESVADDAAYDLGQGAGFYV
                     DATELPWSDHYQMYSYVVDELPALIEANFPASSRRSISGHSMGGHGALVIALRNPGRYA
                     SVSAFSPIVAPTQVPWGQKALAAYLGEKNLEAWKQYDTVELIRVAQERLPLLVDQGLSD
                     EFLEHQLRPQLLRTACEQVDHPLTLNLRPGHDHSYYFISTYLSEHMAHHAAALNR"
                     /name="fghA"
     gene            6378..6665
                     /locus_tag="AhaeAN4_RS17885"
                     /old_locus_tag="AhaeAN4_18015"
     CDS             6378..6665
                     /locus_tag="AhaeAN4_RS17885"
                     /old_locus_tag="AhaeAN4_18015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019383542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="WP_151800610.1"
                     /translation="MEQYFEWDEAKNRKNQKKHDVSFETASLVFDDPLRISMQDRHTDG
                     EERWQTIGKVKGVLMLLVAHTIFDEDDCEIIRIISARQVTKAERDRYEHG"
                     /name="BrnT family toxin"
     gene            6652..6966
                     /locus_tag="AhaeAN4_RS17890"
                     /old_locus_tag="AhaeAN4_18020"
     CDS             6652..6966
                     /locus_tag="AhaeAN4_RS17890"
                     /old_locus_tag="AhaeAN4_18020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006582675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="WP_151800611.1"
                     /translation="MSMVRYSRKELNEKFSDKQDAEIQRLLAKGTVADDQLDLSDIPEI
                     TDWSNAVRHNQFYRPVKQQTSIRLDADVLAWFKSQGKGYQTRMNEILRDAMLKELKNHQ
                     "
                     /name="BrnA antitoxin family protein"
     gene            7193..7510
                     /locus_tag="AhaeAN4_RS17895"
                     /old_locus_tag="AhaeAN4_18025"
     CDS             7193..7510
                     /locus_tag="AhaeAN4_RS17895"
                     /old_locus_tag="AhaeAN4_18025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005065053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004728121.1"
                     /translation="MLGQHHHFLKKLSISDAAIAYRKPRSTILKYIKSGDFELDYNNLI
                     EYSQLVKKLGEITSPIRDERDQQIEKLKDEVMHLKKQLNFFKYTSISNKHRLLISHKDS
                     I"
                     /name="hypothetical protein"
     gene            complement(7750..7974)
                     /locus_tag="AhaeAN4_RS17900"
                     /old_locus_tag="AhaeAN4_18030"
     CDS             complement(7750..7974)
                     /locus_tag="AhaeAN4_RS17900"
                     /old_locus_tag="AhaeAN4_18030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004676219.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_004676219.1"
                     /translation="MPYEEFQRLMGKAGLSIKEFATLLDMNPNSITNYKKIGKVPTHIA
                     VLVYLLSSMKDEGVDFYPIFEKIKSYSKD"
                     /name="helix-turn-helix transcriptional regulator"
     gene            8124..8369
                     /locus_tag="AhaeAN4_RS17905"
                     /old_locus_tag="AhaeAN4_18035"
     CDS             8124..8369
                     /locus_tag="AhaeAN4_RS17905"
                     /old_locus_tag="AhaeAN4_18035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005249014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004282220.1"
                     /translation="MYSYPDSNTEKKIALMIINDFFIQKAHELWIFLQLDQCFNDYEAT
                     LIWTRRYLEKHPECEYSDIQKAFVSCFPEHFFSFDC"
                     /name="hypothetical protein"
     gene            complement(8661..9422)
                     /locus_tag="AhaeAN4_RS17910"
                     /old_locus_tag="AhaeAN4_18040"
     CDS             complement(8661..9422)
                     /locus_tag="AhaeAN4_RS17910"
                     /old_locus_tag="AhaeAN4_18040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017393773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000425105.1"
                     /translation="MEMEKAKKRGRPAQLLQIAELHAFVEFLEQQEQLSDLQSQVLKAL
                     NSVDCNFEGLTQTDQVLVKEALKPYREHLKLKLLFEELNNLPLKTEYEQKFLDLYELFQ
                     KNALDQMELNILKTLATRYLNFKAQKLEYSDLELYLSQLQKKDAGKKRKAENQRKFELG
                     GAVLVAFKKLNIDISNDTPQQITNRIVNTTKFHNEVRKSLIFKDVKTYENEYFKANKLF
                     IQVLEGLHTWQKGGELLSVIEIKKALEKGEE"
                     /name="hypothetical protein"
     Typing          888..1766
                     /name="rep_cluster_1364"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="85.81"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          2..336
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.01e-66"
     Typing          9725..10554
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.585"
                     /coverage="100.0"
                     /evalue="2.36e-176"
ORIGIN
        1 ttttatagtg ctgaaaacaa cccattttca tcattggaag tcatgtatta cctgaattac
       61 ttaatcaaga atgcaacaaa taaggacaaa ctaaaaaatg atttgaatga aattatcaaa
      121 aaccaaggcc ctatacttaa acaagaagct tggtccatct acgaaatgaa tgaaaaacta
      181 gaagactcaa gacgtaacag taccccatgt tttgagttcg aggtgcattc acttcctgaa
      241 tacgtattac cgatacaaaa tacgcctccc aaacgcactc aatcagaacc agtacaacct
      301 aaagctgatg attatgatta cggtggccct ggtttttaag ctcagttggt tttatccaaa
      361 gtttacagag cgagtgtcta cgagcgaaca gacacaatta aaatttttgc catgcttttt
      421 ccctgattac aaactcccct ttgctgctga gactgagata cagagcatcc cggagggtgc
      481 gaacagattt aatttgcatc taggtcgagc gaagcgagta taaaaaactt atgagcgaag
      541 cgaattccga gttgcttttg cttttttgaa gttatcacga gattaaccca aaaattgccc
      601 cttcgaactg agcgtaagcg aagttcaata gagtttgagc gtagcgaaaa catagggcaa
      661 tttttcattc tttccttttt aatatatttt aaatctttta aatcttttca gtcgtctctg
      721 tgccttatat agcaaaggtt tcagcgttta aatatgacgg attgactact aactatgacg
      781 gattgactac taactatgac ggattgacta cttaactatg acggattgac tactaactat
      841 gacaagtata tatttgtcat atattagaaa aatcaatggt tttgttttga ataaaaatca
      901 cgttgtaaaa tcaaatcaag taatagaagc atcgtatcaa ttaagtgccg tagagcaacg
      961 aattgtctta gctgccattt cacggattcc caaaaatcag cctattacag atgatgagtt
     1021 ataccctgtt agcgtcgatg aattaaaaca attgggcgta catgagaaaa ctgcatacag
     1081 agatttaaag gaaggtatta atagactcta tgagaggtct attaacctca gtattgatga
     1141 taaatctata aaaatgagat gggtgcaaga aatccaattt ttagatagtc aaagtgtcat
     1201 tggaatccgt ttttcaaaac ccattctgcc atttatttcc aatctaagta gagaattcac
     1261 caaatatgcc ttatcagaca ttgctgggat caatagtgga tacggtattc gtatttatga
     1321 gctactggtt caatatcggc aaataggtaa gcgtgaaatt tctgttgaga gtttacggac
     1381 aatgttagaa ctcggtaaaa agtacccatt attcgctgat ttcaagaaac gagtaattga
     1441 cactgctata gaccaaatca atgaatatag ccctttaaaa gtcacttatg agcagaaaaa
     1501 aacagggcgt aaagtcactc atattatctt ttcttttaaa gaaaaatcta aatccataac
     1561 acatacctcg aaagattttt atcagctatc tgatgctcaa attaatctat tcgggaatca
     1621 attatcacgt ttacatgagc tttcccattt agcctcagaa ggtgaaagtt atgagatttt
     1681 agctgctaaa attaaggata tgcttagaaa ccctcaccaa caacagcagt ttttgccaca
     1741 cttaaagaac ttagggttta aagcttgagt gtattgaaga atacacttat tgtattcttc
     1801 aatacgtatc ctaaatacac agcaatacac tatgaaaaca agtacaaaat acagtgttag
     1861 tgatgcagcc acactgtata agaaatctcg tgctacactt tataaagata taaaaaatgg
     1921 ggtgttaagt cgtgatcatg atggctctat cgacttctct gagttactcc gcgtttatgg
     1981 tgaacctttt agtggaaaac actcaaaaca cactgatacg tctccaacac aaactataaa
     2041 tacacctgaa tacacagaca atacagttat tgaactaaag aatcagatcg attttctaaa
     2101 gaaacagtta gaaaaagcag aagatcgcga agctaaagca aattctcgta ttgatacatt
     2161 acttacccta attgaaatga agccaactca ggttcatccc acaccaactt cagatcaatc
     2221 ttcagtgcaa tctagtccat ctcaagtaga acctacattg aatgtagata acgacaaaca
     2281 tgacggattg actactccag agaataagcg cattcctgtt ccagagcatg ttgaaccaga
     2341 gcctaaaaag cgtggcttct ggagtcgttt ttttcttcca tatggttaac ttaggctaaa
     2401 taaaaccgtg gaacattgct caataaataa gcgtggaacg tgaaaaatca taaaaaaaac
     2461 ccaccttggg gaagatgggc tataaactag aaactacagc attgatttct atagataatt
     2521 ataacgcatt tcgtataagg tgtattatgt taattttata aaaacttaaa aatagatata
     2581 gaatttttta attataagct tttatcaatt attaatttta taaaggcttc ttgtcattct
     2641 atatactata gggtactata ctataagtac tatactataa taaaatttaa ggaattagct
     2701 ttatgccaaa tcaagtcgaa gataaaaaaa agattctgac gcgtgtcaga cgaatcaagg
     2761 gtcaggctga agcgattgaa aaagctttag aagacaatgt ggaatgtggt gcaattctgc
     2821 aacaaatatg ctctgtgcgt ggtgctatta atggtttaat gaatgaaatg ttggaggttc
     2881 atttaaagga cactttagta tcaggtgaaa ccacagaaca acaacgaaaa gaagaattgg
     2941 ctgaaatcgc caagattcta aaatcatatt taaaatagat ctgtataagg aattattcca
     3001 tgaagtcacg tgctgctgtt gcgtttggtc ctggtcaacc tcttcaaatt gttgaggttg
     3061 atgttgcacc accgaagaaa ggtgaagtat taatcaagat ctcccatact ggtgtgtgtc
     3121 atacagacgc atttacttta tctggcgatg atcctgaagg cgtttttccg gctattctcg
     3181 ggcatgaagg tgctggtgtt gtagttgagg ttggtgaagg cgtgactagc gtcaaaccag
     3241 gtgatcatgt tattccactt tacactgctg aatgtggcga gtgcgtattc tgtaaatctg
     3301 gaaaatcgaa cttgtgtatt tccgttcgcg aaactcaagg tagaggcgta atgccagatg
     3361 gtaccactcg cttttcctac aatggtcaac cgatttacca ctatatgggc tgctcaacgt
     3421 tcagtgaata cactgtagtg gcagaagtat cattggctaa aatcaatcct gaagccaatc
     3481 acgaacatgt ctgcttactg ggttgtgggg taacaaccgg tatcggtgca gtacataaca
     3541 cggctaaagt acaagaaggt gatagtgttg cggtctttgg tttaggtggt attggtttgg
     3601 cagtcgtaca agccgcacgt caagctaaag cagggcgtat tattgtggtt gacatgaatc
     3661 cagataaatt tgcgctggca aaagagtttg gtgcaacgga cttcttgaac cctaaagact
     3721 acgataaacc aatccagcaa gtcattgtcg aaatgactgg ctggggtgta gatcattcat
     3781 ttgaatgtat tggcaacgtg aatgtaatgc gtgcagccct tgaaagtgca caccgtggct
     3841 ggggacaatc cgtaattatt ggtgtagctg gcgcaggtca ggaaatctct acccgtccat
     3901 tccaactggt tactggtcgt aaatggctag gttctgcctt tggtggcgta aaaggtcgta
     3961 cgcaacttcc aggaatggtt gaagatgcca tgaaaggtaa aattcaatta gaaccatttg
     4021 tgacacatac gatgggcttg gatcagatca atgaagcctt tgatctaatg catgaaggta
     4081 agtcaattcg cactgtcatt cactattgat ccacggatca atacctaatt tttcaaaacc
     4141 aaccatcaag gaaacacttt aatggctact ccagtaattt ccggtaacga tattttctcc
     4201 catgtcttcc tcggcgcagt cgaccttaag aagtcggttg tattctacga tgccgctcta
     4261 ggtgccctcg gcattaaaaa cctagggcca ttcggtaacg attgggttct gtacggtcgc
     4321 gacaaacctg ctttcatgat cacccgccca agcaatggcg cagaacccac cagtaatggt
     4381 gtgacggtag gcttcgcagc tgccacccct gcagaggtaa atgcattcca cgcagccggc
     4441 ctcgccgctg gtggcaccga tgaaggtcag cctggcccac gtggccacct gccgggtgct
     4501 tacgcggcct acctgcgtga tccggctggt aataaggtct gcgcctacac ctttgtctaa
     4561 aacctaggag aagtgaggtc attgtgtaag gactggttcc agccaaaaac aatctcatct
     4621 tccactgcaa ccgtgtggca tgcccgctca tgccacacgg tttacggtac tctgagcctc
     4681 tatctgctga ttagagctca gaaaggctgt tacctcgcct tcatagccaa agacggtcaa
     4741 tgagcacgat cggactgaaa gttcggtgcg tgatgcaaga aacacagaat ccaaaatgag
     4801 catcgcggta gtggctccct gaattacaca ctttattgtt tgtacattga agtcgacatc
     4861 cgaccttcgt ggtgtggtgg cgtctgggtt tgtagcagcg atcttcatgc cagcgacaac
     4921 gctgaaaacc tgctgagtgg tgcggtgaag catttttaac taatcctgga tggaacgaaa
     4981 attcgtcttg attggcgcct gagcaccacc tcgttgaaat aaggagaaaa tccgtggaac
     5041 gcattgaaca tcacgccagc ttcgagggtt ggcaggacgt ttttcagcat gagtcaacag
     5101 tgcttggctg catgatgagg tttggaatct atcttccgcc tcaggctgag catgaaaagc
     5161 tcccagttct gtactggctc tcaggtctga cctgtaccga acagaacttc atcaccaagg
     5221 ctgcggtgca gcggtatgcc gcggagcacg gaatcattgt cgtagcccca gataccagcc
     5281 cgcgcggaga aagcgttgca gacgatgctg cctatgatct aggtcaaggt gcgggtttct
     5341 atgtagacgc cacagagcta ccttggagcg atcactatca gatgtattcc tatgtggtcg
     5401 acgaattacc agcgctgatc gaagcgaact tccctgcgtc gtcaagacgc agcatcagtg
     5461 ggcactccat gggcggtcat ggcgctctgg ttattgcgct tcgcaacccc ggtcgctatg
     5521 caagtgtctc ggcgttctcg ccgatcgtgg cgcctacgca agttccatgg gggcagaagg
     5581 ctctggcggc ttacttgggc gaaaagaatc tggaagcctg gaagcaatat gacacggtcg
     5641 agctgattcg cgtggcgcaa gaacgcttac cgctgcttgt cgatcaaggg ctgagcgatg
     5701 agtttctcga gcaccagctt cgaccgcaac tgttgcgtac cgcgtgtgaa caggtagacc
     5761 acccgctaac gctcaatctg cggcctggtc atgaccatag ctactacttt atttcgacct
     5821 acttgagcga acacatggcc caccatgcgg ctgcattgaa tcgctgaaca ttgggagacc
     5881 ctgacttgga aatgtctgta taggacattt ctatttggtt ttaaggggct ttgttgcata
     5941 aatacataac caactgattt tgttcattta atttttaggt tgaaattcat acaaacctat
     6001 ttaaatcaga agcttacaaa atctttatgc aacaaagccc atatcagata aaaaagtaga
     6061 ggagctaata ctaagccgtg atttcttgga aaagctgaaa gagctagtta atcgaacaga
     6121 aataccatgt agttaactat ccatttaaca taatggcgat tatacgcaat acacttgtat
     6181 attaatttaa atatcaacta cttaataaaa atataaaaat ccaaaaatgc ccaaaaagcc
     6241 gattttgaaa caagtcggct tttttgtgag cagttttgga atgttttgcc aataaatttg
     6301 actaaaaatt acgcttttca acttgagtga tacaattttg tgatacatta ttgtatatac
     6361 aaatttgaat gttttttatg gaacagtatt tcgaatggga tgaggcaaag aatcgaaaga
     6421 atcagaaaaa acacgatgtc tctttcgaaa cggcaagcct tgtttttgat gatccactga
     6481 ggatctcaat gcaggataga cataccgatg gtgaagaacg ttggcagacc attggaaaag
     6541 taaaaggcgt actaatgctc ttagtagctc acaccatctt tgatgaagat gactgtgaaa
     6601 tcatacgaat cattagtgca agacaagtca ctaaggcgga gcgagataga tatgagcatg
     6661 gttagatact cacgcaaaga actgaatgaa aaattcagcg acaagcaaga tgctgaaatt
     6721 caacgcttgc ttgctaaggg aacggttgct gacgatcagc tagatctatc agatattcct
     6781 gaaattaccg actggagcaa tgctgtacgc cataatcagt tctaccgtcc agtgaagcaa
     6841 cagacttcca ttcgtttaga tgctgatgta cttgcatggt ttaaatctca aggcaaaggt
     6901 tatcaaacac gaatgaatga aattctgcga gatgctatgc tgaaagaatt aaaaaatcat
     6961 caataaaaat gggagcttaa gctcccattt ttgacttcgc ataacgtgta ttatgttaat
     7021 aatataaatt cttaaaagcg tattgattta ataatagaaa aattatcaga gtagggtatg
     7081 tgtaaagtaa acgttcaatt atcaaccaat aatgattgaa gatttatatc ttacatttgt
     7141 taattttatt aatatatatc tatcaaatta ataatgtctt aagttaaata atatgttagg
     7201 tcaacatcat cattttttaa aaaaactttc gatcagtgat gctgctatag cgtatagaaa
     7261 acctcgctcg actatactta aatatataaa aagcggagat ttcgaacttg actacaataa
     7321 cttaattgaa tatagccagt tagttaaaaa attgggtgaa attacttccc ctatacggga
     7381 cgaaagagat caacaaatcg aaaaactaaa agatgaagtt atgcatttaa aaaaacaact
     7441 taattttttt aaatatactt caatctcaaa caaacataga ttattaattt ctcataaaga
     7501 ctccatttaa cataatagag cttatgcgaa attcagttgt aatccccaaa tagaaatgtc
     7561 cggtttaccg attgctcgac ctaagatctc aaaacataag aatttgatga tcaccctggt
     7621 tctcaaaact cgatacttta ttgaaaagtt ctatcgaggc ttataagtcc ctgaaacaaa
     7681 aaaatgcctg aacttcaggc attttttacg tcatcactaa cagcttacaa caatcaacaa
     7741 acgagagttt tagtcttttg aatacgactt aattttttca aaaatagggt aaaagtctac
     7801 accctcatct ttcatgctag aaagtaagta aaccagaaca gcgatatgag taggtacttt
     7861 tccgatcttt ttatagttag ttatggagtt tggattcatg tccaataagg ttgcaaattc
     7921 cttaattgaa agacctgctt tccccataag tctttgaaat tcttcgtatg gcatcgtgat
     7981 aaatacaatc tgtttgaatt agaagtataa cacagactat aaaacttaaa aaaacacata
     8041 aaaaatgtga caaacacatt ttttatgtgt tataaatagc ttatcaacta acctatttat
     8101 ccttactatc ggagaaatgt aatatgtact catacccgga ttcaaacact gaaaagaaaa
     8161 ttgctctcat gatcatcaat gactttttca ttcaaaaagc ccatgaactg tggatctttt
     8221 tacaactcga tcagtgcttt aatgattatg aagccacact gatttggact cgacgttatt
     8281 tagagaagca tcctgagtgt gagtacagcg atattcaaaa agcctttgtt tcttgtttcc
     8341 cagagcactt cttcagcttt gactgttaat ttatgagagg cacggtaaaa aatcgcaatg
     8401 ggtaagtaaa tcccaaatag aaagagccag ttcatacaac tggctctttc tattgtcacg
     8461 tataaatcta agactttctt tgagcatact aaaattgatg ttccacggct tctaaaacat
     8521 tgattttagg ttcaggattg gggtcgccat gacgttactt tagggtgtac tctaaattta
     8581 cataatgtct ctgtatacga aattcgattg taagcccaaa atagaaatgt ccaacttctc
     8641 taaaatccct gctaaataaa ctactcttcg cctttttcta aggccttttt aatttcaatt
     8701 actgacaata attctccgcc cttttgccag gtatgtaatc cttctaacac ctgaataaaa
     8761 agtttatttg ctttaaaata ttcattttca tatgtcttaa catctttaaa aattaaagat
     8821 ttacgtactt cattatgaaa ctttgtcgta ttcacaatcc gattggtaat ctgttgagga
     8881 gtatcattgc taatatcaat attgagtttc ttaaatgcga caagaacagc accacctaat
     8941 tcaaatttac gttgattttc agctttgcgt tttttcccag catccttctt ctgtagctga
     9001 ctcaaataca attctaaatc actgtattca agcttttgcg ctttaaaatt cagataccgt
     9061 gtcgccaagg tttttaaaat atttagttcc atctgatcta gtgcattctt ttgaaaaagt
     9121 tcatacagat ctaaaaactt ctgttcatac tcggttttaa gaggcagatt attcaactct
     9181 tcaaacagca atttcaactt cagatgttca cgataaggtt ttaaagcctc tttgaccaga
     9241 acctgatctg tctgagttag cccctcaaaa ttacaatcaa cactatttaa agcctttaac
     9301 acttgagatt gaagatcaga aagctgttct tgctgttcta aaaactctac aaatgcatgt
     9361 aactcagcaa tctgtaacaa ctgagcaggt cgaccacgct tttttgcttt ttccatttcc
     9421 atcatctgcc gccaaatttt cagactgccc tctttaaata tggttttacc atatttaaag
     9481 agggcagtct gaaaataatt atttaatata aaaataaaat ttcttgaaaa gaatttttat
     9541 ttttatatta aataattcgc acttgacagt ttctcctcat cggagaaact agtacgcaat
     9601 ggggcgcttc ccccttgacc cccttcaacc ggatttttca gccatacagg catttcaaat
     9661 ccgggcggtt ttcattccgt ttcactgcat aaaaaccgaa cccacaaccc agcagaggtg
     9721 aacatggcca gctaccactt cagcgtcaaa agcaaaaata agggctatgc gaccggccat
     9781 tatctctaca tttcccggct gatgcagtat gaaggcatcc gcaaaaccag caatgagacc
     9841 atagaacata ttgaacctgg tcgatgtatg cctgcctttg taaaagatcc cattgatttt
     9901 tggcaggcag cagatacgta tgaacgagcc aatgccaagg cctacatgga gtatgaaatt
     9961 gccctaccga atgagttcac gtcagaacag cgtaaaacgc tcatagagac gtttttagac
    10021 aaacatatag ttccacagca atatccgcac tcatacgcca tacacaacgt aaaatcacgt
    10081 atcagtggcg aagatcagcc tcattgtcat ctgatgttct cgctaaaagc ggatgacggc
    10141 attgaacgca gcgcagaaca gtacttcaaa cgctataacc ctcaagatcc agccaaaggc
    10201 ggtgccaaaa aaatccaact tcaagatggc catgaagact attccacttt tttgatctat
    10261 atccgaaaac aatgggaaaa tcatttgaat gatgcccttg cacagcactg cccgaccgtc
    10321 acctacacac tggatggcca agacatcacc atcaaaaatc aggtttcagc agacagctat
    10381 gaaaaatata acgagatcca cagcacgcta taccttccag agccaaaact aggcgttgga
    10441 caacaaaatg cgacagcgga atatctcgcc caaatccaaa aaatcagaga acacaaccag
    10501 caagaacgag aactcgaaga acgccaaaag caattagatc aacaacatga atat
//
