LOCUS       NZ_CP031925             4148 bp    DNA     circular CON 19-JUN-2024
DEFINITION  Escherichia coli O26:H11 strain FWSEC0001 plasmid unnamed8, complete
            sequence.
ACCESSION   NZ_CP031925
VERSION     NZ_CP031925.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN08768102
            Assembly: GCF_005037725.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli O26:H11
  ORGANISM  Escherichia coli O26:H11
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4148)
  AUTHORS   Tyson,S., Peterson,C.-L., Olson,A., Tyler,S., Cabral,J., Lynch,T.,
            Knox,N., Van Domselaar,G. and Graham,M.
  TITLE     Food and Water Consortium WGS
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4148)
  AUTHORS   Tyson,S., Peters,G.A., Melnychuk,K., Laminman,V. and Kaplen,B.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-AUG-2018) National Microbiology Lab, Public Health
            Agency of Canada, 1015 Arlington street, WINNIPEG, MB R3E3R2, Canada
REFERENCE   3  (bases 1 to 4148)
  AUTHORS   Cabral,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2017) National Microbiology Laboratory, Public
            Health Agency of Canada, 1015 Arlington St, Winnipeg, Manitoba R3E
            3R2, Canada
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: AUG-2018
            Assembly Method                   :: Unicycler;Canu v. 0.4.4.0;1.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 66.48x;325.05x
            Sequencing Technology :: Illumina MiSeq;Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_005037725.1-RS_2024_06_19
            Annotation Date                   :: 06/19/2024 09:52:42
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,967
            CDSs (total)                      :: 5,833
            Genes (coding)                    :: 5,508
            CDSs (with protein)               :: 5,508
            Genes (RNA)                       :: 134
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 101
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 128 of 325
            Pseudo Genes (incomplete)         :: 202 of 325
            Pseudo Genes (internal stop)      :: 70 of 325
            Pseudo Genes (multiple problems)  :: 66 of 325
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP031925.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4148
                     /organism="Escherichia coli O26:H11"
                     /mol_type="genomic DNA"
                     /strain="FWSEC0001"
                     /serotype="O26:H11"
                     /isolation_source="Clinical: Human (Homo sapiens)"
                     /host="Homo sapiens"
                     /db_xref="taxon:244319"
                     /plasmid="unnamed8"
                     /geo_loc_name="Canada:British Columbia,Vancouver"
                     /collection_date="2002"
                     /collected_by="National Microbiology Laboratory: Public
                     Health Agency of Canada"
     gene            1..1140
                     /locus_tag="B9S25_RS30210"
                     /old_locus_tag="B9S25_031625"
     CDS             1..1140
                     /locus_tag="B9S25_RS30210"
                     /old_locus_tag="B9S25_031625"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761319.1"
                     /GO_function="GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase family protein"
                     /protein_id="WP_032341919.1"
                     /translation="MNKPRFNGTPVASLDSLSAMLGIERKRLDWIVKSVSMSYKQFKVE
                     TGKNKKERQIFEPKRSLKGIQKKINKEIFEKIDYPHYLHGALSGRDYISNAAVHTRKRT
                     VICLDITNFYPSISKKDVCSIFKNLMRFSPEVSLCLTELVTLNNKVPQGGCCSSYIANL
                     LFFNSEYNLYNRLKSMGLSYSRLLDDITISSDKDLSSEEKTKVIKLVHGMVNQYRLSIN
                     ESKTTIEHSKDSSSKLSVTGLWVKHGVPKLTKENRRYIRYLVYICKKQGAYERHTKEYH
                     DLWNRCSGKVAQMSRLGHVQAVELRAILSEIMPVYDDYKISKLKLMAKHYLNKFTPPLT
                     DEQIRKIDRMLYDFDIVGRTNKNLAKLYRRKLVALLPDR"
                     /name="reverse transcriptase family protein"
     gene            1145..1348
                     /locus_tag="B9S25_RS30215"
                     /old_locus_tag="B9S25_031630"
     CDS             1145..1348
                     /locus_tag="B9S25_RS30215"
                     /old_locus_tag="B9S25_031630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000427298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="retron Se72 family effector protein"
                     /protein_id="WP_000427298.1"
                     /translation="MENGFVNFYDHVKGYGFIRRERGRDVFFRYDDFLFLGHDVDICKG
                     ILVRFKLEKTDKGFKAVAIQKV"
                     /name="retron Se72 family effector protein"
     gene            complement(2185..2379)
                     /locus_tag="B9S25_RS30225"
                     /old_locus_tag="B9S25_031640"
     CDS             complement(2185..2379)
                     /locus_tag="B9S25_RS30225"
                     /old_locus_tag="B9S25_031640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_001041640.1"
                     /translation="MNKQQQTVLNMAGFIKSQSLTLLEKLDALDADEQAAMCEKLHELA
                     EELQNSIQTRFEAENRTGI"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            2664..2987
                     /gene="mobA"
                     /locus_tag="B9S25_RS30230"
                     /old_locus_tag="B9S25_031645"
     CDS             2664..2987
                     /gene="mobA"
                     /locus_tag="B9S25_RS30230"
                     /old_locus_tag="B9S25_031645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052604.1"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein MobA"
                     /protein_id="WP_000166966.1"
                     /translation="MTKRSGSNTRRRAISRPVRLTAEEDQEIRKRAAECGKTVSGFLRA
                     AALGKKVNSLTDDRVLKEVMRLGALQKKLFIDGKRVGDREYAEVLIAITEYHRALLSRL
                     MAD"
                     /name="mobA"
     gene            3237..3575
                     /locus_tag="B9S25_RS30235"
                     /old_locus_tag="B9S25_031650"
     CDS             3237..3575
                     /locus_tag="B9S25_RS30235"
                     /old_locus_tag="B9S25_031650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001027011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001027011.1"
                     /translation="MNISIKNNDLKTIARFDGKTLRDAFSRKIATFDGKILRDASLKKI
                     ATFDGKVLRDASLKKVATFDGKVLRDASLKKVATFDGQNLRDASSKIILSADAEVGIVV
                     MAFVAELI"
                     /name="hypothetical protein"
     Typing          2415..2655
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="99.174"
                     /coverage="100.0"
                     /evalue="1.65e-119"
     Typing          complement(1548..1996)
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.778"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          2665..2984
                     /name="MOBF"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.065"
                     /coverage="100.0"
                     /evalue="3.33e-62"
ORIGIN
        1 atgaataaac ccaggtttaa tggaactcct gttgcatctt tggattcttt atctgcgatg
       61 ttaggcatag aaagaaaaag attggactgg atagtcaagt ctgtatcgat gtcgtacaag
      121 cagtttaagg ttgaaacagg gaagaataaa aaagaaaggc aaatttttga acccaagaga
      181 tctttgaaag gaattcaaaa aaaaataaat aaagaaattt ttgagaaaat tgattaccct
      241 cattatttgc atggggcgtt aagtggtcgc gattatatat ctaatgccgc tgttcatact
      301 aggaaaagaa cagttatatg tcttgatatc accaattttt atcccagcat tagtaaaaaa
      361 gatgtttgtt ctatttttaa aaatttaatg cgtttttctc ctgaggtttc tttgtgctta
      421 acggaacttg tgacgttgaa taataaggtt cctcagggtg ggtgctgttc tagttatatt
      481 gcaaatcttt tattttttaa tagtgagtat aatctctata atagattgaa gtctatgggg
      541 ctttcatatt caaggctttt agatgatatt actatatcct ctgataagga tctttcatca
      601 gaggaaaaaa caaaagtaat aaaactcgtt cacggaatgg tgaatcagta cagactttca
      661 ataaatgaaa gtaagacaac gatagagcac tcaaaagatt cgtcttctaa acttagtgtc
      721 actgggttgt gggtaaagca tggcgttcct aagttaacaa aagaaaatag acgttatatc
      781 aggtacttag tttatatatg taaaaaacaa ggtgcttatg aacgtcatac gaaagagtac
      841 catgatttat ggaatagatg ctctggcaag gttgctcaaa tgtcgaggct tgggcatgtc
      901 caggcggttg aattgagggc gattctttct gagataatgc cagtttatga tgattacaag
      961 atatctaaat taaagttgat ggctaagcat tatctaaata aatttacgcc gcctttaact
     1021 gatgagcaga taaggaaaat agataggatg ctctatgatt ttgatatagt agggcggact
     1081 aataagaatc ttgcaaaact atatagacgt aagcttgtgg cattacttcc agataggtga
     1141 ttatatggaa aatggatttg ttaattttta tgaccatgtt aaagggtatg gctttataag
     1201 gcgtgagcgt ggacgtgatg ttttctttcg gtatgatgat tttttgtttt tagggcatga
     1261 tgtcgatata tgtaaaggta ttttagttag gtttaaatta gaaaaaacag ataaagggtt
     1321 taaagctgta gcgattcaaa aggtataaca tcgtggatgt gtcagcttgt ttttatgata
     1381 tgagaacttg agatcctttc tatctcgtcg taatctcttg ctgtgtaaac gaaaaaaccg
     1441 ccttgcaggg cggtttttcg aaggttctca gagctaccaa ctctttgagc cgaggtaact
     1501 ggcttggagg agcgcagtca ccaaaacttg ttctttcagt ttagccttaa caggcgcatg
     1561 acttcaaggc taactcctct aattcggtta ccagtggctg ctgccagtgg cgataagtcg
     1621 tgtcttctcg gtttggattc aagtcgatag ttaccggata aggcgcagcg gtcggactga
     1681 acggggggtt cgtgcataca gtccagcttg gagcgaacga cctacaccgg gccgagatac
     1741 caacagcgtg agctatgaga aagcgccacg tttcccgaag ggagaaaggc ggacaggtat
     1801 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     1861 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg gtttttgtga
     1921 tgctcgtcag gggggcggag cctatggaaa aacgcctcct gctcgccctt cttctgggcc
     1981 tttgtctttt gctcacatgt tctttccggc tttatcccct gattctgtgg ataaccgtat
     2041 taccgccttt gagtgagttg acaccgctcg ccgcagtcga acgaccgagc gtagcgagtc
     2101 agtgagcgag gaagcggaag agcgccttat gtgacatttt ctccttacgc tcctctatgc
     2161 cgttctgcat cctgtcctga tgcgttatat cccggtgcga ttttccgctt caaagcgtgt
     2221 ctgtatgctg ttctgaagtt cttccgcgag ttcgtgcagc ttctcacaca tggctgcctg
     2281 ttcgtcagca tcaagtgcgt ccagtttttc gagcagcgtc aggctctggc tttttatgaa
     2341 tcctgccatg ttgagtacgg tttgctgctg cttgttcatc tttctgtttt ctccgttctt
     2401 tctgtcatct gctttgtgtg attatatcgc gtaccacttt tcgactgttt taccgtcggt
     2461 attctgctgt ctgactcttt gacgggcatt tctgtcagac aacactgtca ctgccaaaaa
     2521 actgccgtgc ctttgtcggt aattcgagct tgctgacagg acaggatgtg taattgttat
     2581 accgcgcata catgcacgct attacaatta ccctggtcag ggctttgccc cgacacccca
     2641 tgtcagatac ggagccatgt tttatgacaa aacgaagtgg aagtaatacg cgcaggcggg
     2701 ctatcagtcg ccctgttcgt ctgacggcag aagaagacca ggaaatcaga aaaagggctg
     2761 ctgaatgcgg caagaccgtt tccggttttt tacgggcggc agctctcggt aagaaagtta
     2821 actcactgac tgatgatcgg gtactgaaag aagttatgag actgggggcg ttacagaaaa
     2881 aactctttat cgacggcaag cgtgtcgggg acagggagta tgcggaggta ctgatcgcta
     2941 ttacggagta tcaccgtgcc ctgttatcca ggcttatggc agattagctt cccggggaga
     3001 aaactgtcga aaacagacgg tatgaacacc gtaagctccc aaagtgatca ccattcgctt
     3061 tcatgcatag ctatgcagtg agctgaaaac gatcctgacg catccttcct gttttttcgg
     3121 ggctaaacct ctctttttgc ggtatttgcg tcagaattgc gttcagcgcg tttcagcggt
     3181 gcgtacaatt aagggattat ggtaaataca attggttaaa ttactattgg tgataaatga
     3241 atatttcaat aaaaaacaat gacttaaaaa ctattgctcg gtttgacggt aaaaccttaa
     3301 gagatgcctt ttcgagaaaa atagcgactt ttgatggcaa gattttaagg gatgctagct
     3361 taaaaaaaat agcgactttt gatggcaagg ttttaaggga tgcctcttta aaaaaagtgg
     3421 cgacttttga tggcaaggtt ttaagggatg cctctttaaa aaaagtagca acctttgacg
     3481 gtcagaattt aagagatgct agttcaaaaa ttatcctgag tgccgatgct gaggttggga
     3541 ttgttgttat ggcctttgta gctgagttga tttgaatctc atgctgcata ccttttagcc
     3601 tatcgctggc aggataaggc gctttcttgt tggtgacaga aagataaaaa actttataat
     3661 aatcagcgaa gcgggcgaac cacgcctggc tctcgctctt taacaaatgg ttacgtgtat
     3721 gttgcgcaag gaaaccgatg ccttgttttt aaggcgatct cgcatgagat catgaccggc
     3781 ctggccgcta attaatggtg agaacgcccc tgagcgtgta tcagcactaa aagaatagcg
     3841 acgattgtct ggcaatgtga gttaaaagtc ccaaagagcc aatagagttt ttccttgctt
     3901 tcctactggt ctggcaaaat ttcataacca ataggagttg catcatgttt attatcaaca
     3961 tcaacattaa cgtaaccaag agctcttcat cttgttctgg agacggtcag atgaagaaaa
     4021 aaagagaggt tggctctctg gaggcactga aaaaaacaca gtttttgttt tctatgtgct
     4081 tgattatgtt taaagtaata gattacgtat cgaaactttt taactaatcg acttttaact
     4141 caaacatc
//
