LOCUS       NZ_CP031924             6715 bp    DNA     circular CON 19-JUN-2024
DEFINITION  Escherichia coli O26:H11 strain FWSEC0001 plasmid unnamed7, complete
            sequence.
ACCESSION   NZ_CP031924
VERSION     NZ_CP031924.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN08768102
            Assembly: GCF_005037725.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli O26:H11
  ORGANISM  Escherichia coli O26:H11
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6715)
  AUTHORS   Tyson,S., Peterson,C.-L., Olson,A., Tyler,S., Cabral,J., Lynch,T.,
            Knox,N., Van Domselaar,G. and Graham,M.
  TITLE     Food and Water Consortium WGS
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6715)
  AUTHORS   Tyson,S., Peters,G.A., Melnychuk,K., Laminman,V. and Kaplen,B.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-AUG-2018) National Microbiology Lab, Public Health
            Agency of Canada, 1015 Arlington street, WINNIPEG, MB R3E3R2, Canada
REFERENCE   3  (bases 1 to 6715)
  AUTHORS   Cabral,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2017) National Microbiology Laboratory, Public
            Health Agency of Canada, 1015 Arlington St, Winnipeg, Manitoba R3E
            3R2, Canada
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: AUG-2018
            Assembly Method                   :: Unicycler;Canu v. 0.4.4.0;1.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 66.48x;325.05x
            Sequencing Technology :: Illumina MiSeq;Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_005037725.1-RS_2024_06_19
            Annotation Date                   :: 06/19/2024 09:52:42
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,967
            CDSs (total)                      :: 5,833
            Genes (coding)                    :: 5,508
            CDSs (with protein)               :: 5,508
            Genes (RNA)                       :: 134
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 101
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 325
            CDSs (without protein)            :: 325
            Pseudo Genes (ambiguous residues) :: 0 of 325
            Pseudo Genes (frameshifted)       :: 128 of 325
            Pseudo Genes (incomplete)         :: 202 of 325
            Pseudo Genes (internal stop)      :: 70 of 325
            Pseudo Genes (multiple problems)  :: 66 of 325
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP031924.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6715
                     /organism="Escherichia coli O26:H11"
                     /mol_type="genomic DNA"
                     /strain="FWSEC0001"
                     /serotype="O26:H11"
                     /isolation_source="Clinical: Human (Homo sapiens)"
                     /host="Homo sapiens"
                     /db_xref="taxon:244319"
                     /plasmid="unnamed7"
                     /geo_loc_name="Canada:British Columbia,Vancouver"
                     /collection_date="2002"
                     /collected_by="National Microbiology Laboratory: Public
                     Health Agency of Canada"
     gene            1..1158
                     /locus_tag="B9S25_RS30160"
                     /old_locus_tag="B9S25_031580"
     CDS             1..1158
                     /locus_tag="B9S25_RS30160"
                     /old_locus_tag="B9S25_031580"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001219937.1"
                     /GO_function="GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO:0003677 - DNA binding [Evidence IEA]; GO:1904047 -
                     S-adenosyl-L-methionine binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="WP_001219937.1"
                     /translation="MRIIDLFSGCGGLSLGFLKGGFDVVGAYDFWDPAIECYRDNFSHP
                     IKKLDLSNVDDVVRELKDIDFDMIIGGPPCQDFSHAGLRIEGARANLTRSFSEIIKRIK
                     PKWFVMENVDRALRSGAYLEARGIFKESGYGLTEIVLDASKCGVPQKRKRLFVIGKLDV
                     RDGFILNEVMCGISKDSMTVRNYLGDSLGIEYYYRHPRNYNRRAIFSIDEPAPTVRGVN
                     RPIPDGYLGHAGDPVSISENVRPLTTFERARLQTFPEDFKFKGAKTNLEQMIGNAVPVE
                     LAKYVAVTIMEYEKKQVKGIYDKEGFRAWLLNEKKLTKRTSSDIISRCCRGVSFFDSEG
                     VDFYNCEIDEIIMKLERLESFVRLGVSLKSQLRRAFKLYYEYCRR"
                     /name="DNA cytosine methyltransferase"
     gene            complement(1386..1544)
                     /locus_tag="B9S25_RS31410"
     CDS             complement(1386..1544)
                     /locus_tag="B9S25_RS31410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001557254.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPERHDLTPLAAATGNWTRWIEIL
                     RVLEVVA"
                     /name="hypothetical protein"
     gene            complement(2204..2395)
                     /locus_tag="B9S25_RS30170"
                     /old_locus_tag="B9S25_031590"
     CDS             complement(2204..2395)
                     /locus_tag="B9S25_RS30170"
                     /old_locus_tag="B9S25_031590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165984.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGEDL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            2460..2783
                     /locus_tag="B9S25_RS30175"
                     /old_locus_tag="B9S25_031595"
     CDS             2460..2783
                     /locus_tag="B9S25_RS30175"
                     /old_locus_tag="B9S25_031595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000955993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000955994.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIEGELRSLRQVVREQGAR
                     DDC"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            2992..>3423
                     /locus_tag="B9S25_RS31125"
                     /pseudo=""
     CDS             2992..>3423
                     /locus_tag="B9S25_RS31125"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            3461..3979
                     /locus_tag="B9S25_RS30185"
     CDS             3461..3979
                     /locus_tag="B9S25_RS30185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_001447511.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNDEFRKLGESVSEAVTSNE
                     TKIRDAIALFTASTEKSLEKHREGVKEAMMQHRKDVLKLAGNTGMMLLGIVFLLFTASG
                     GTLWYLGGRIQANLEEIRKQEETLQKLNAKTWGVEFVQDGRRKFLVIPQGMSAEVTHYQ
                     GKDWIQLTE"
                     /name="MbeB family mobilization protein"
     gene            3986..4219
                     /locus_tag="B9S25_RS30190"
     CDS             3986..4219
                     /locus_tag="B9S25_RS30190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_001448503.1"
                     /translation="MTELETHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSERTERLSQLYSENWR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(4594..4800)
                     /locus_tag="B9S25_RS30195"
                     /old_locus_tag="B9S25_031610"
     CDS             complement(4594..4800)
                     /locus_tag="B9S25_RS30195"
                     /old_locus_tag="B9S25_031610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001096539.1"
                     /translation="MAKLPQEKPKEVIQAGFDDVKKAYKKGAKTVNDTYAEQAGRDLQS
                     GDGKLMATYRRNKKAIDKLDAEG"
                     /name="hypothetical protein"
     gene            complement(4793..5176)
                     /locus_tag="B9S25_RS30200"
                     /old_locus_tag="B9S25_031615"
     CDS             complement(4793..5176)
                     /locus_tag="B9S25_RS30200"
                     /old_locus_tag="B9S25_031615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000065853.1"
                     /translation="MSNIVHLSDKKQKNKPVDISFDDYTDNLSKGFVKNLSNKQKKELI
                     SKIKAILKSRNVAFRSTPPSDWKEKSRTFAIFFPEKKIVFTLGVVNKLKIEDGWINHKI
                     SDTEDAVLSVGKIINLIEDSQHG"
                     /name="hypothetical protein"
     gene            complement(5567..6628)
                     /locus_tag="B9S25_RS30205"
                     /old_locus_tag="B9S25_031620"
     CDS             complement(5567..6628)
                     /locus_tag="B9S25_RS30205"
                     /old_locus_tag="B9S25_031620"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034299.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0009036 - type II site-specific deoxyribonuclease
                     activity [Evidence IEA]"
                     /GO_process="GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HindVP family restriction endonuclease"
                     /protein_id="WP_044862115.1"
                     /translation="MSKIESLYGLKNTNRKESDLWGKNQFNSSFPTSLACYMRDNKMNP
                     IYLYLDKDLKVHAKEVSFDFVFNTTLKNSDLEFQFEKKYEPYQQYAYDDIKGIDLVIKG
                     NNSFLKALEIKLTVLPDSTTYKENESEWSSEIVIRPASTSYCALGIMHSCSDKFHEIRK
                     IFESTCESIQHWGNETEVLSKKENILNCLNTFQENFLDYQKPFLMQPLWKTKGKSPILD
                     QNAFDIFIWSDFALCRTFIDRSSYNNSSASRLMRSSARLARMVYEIASKNKTNINRIYT
                     EMNFGYQSDKEFSLNGKITKAYMVHPRRTKPILPPSVLKEIILNGGQNKLSPERRFDQS
                     VYYTASHLFDEIK"
                     /name="HindVP family restriction endonuclease"
     Typing          2042..2126
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="97.674"
                     /coverage="100.0"
                     /evalue="6.569999999999999e-34"
     Typing          complement(1473..2022)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.298"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          2428..2777
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.162"
                     /coverage="99.0"
                     /evalue="1.96e-64"
     Typing          2774..4317
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.529"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 atgagaataa ttgatctttt tagtgggtgt ggggggttgt ccctaggctt tttgaagggg
       61 gggtttgatg ttgtaggtgc ttatgatttc tgggatcctg ctattgaatg ttacagagat
      121 aattttagcc atccgataaa aaaactagat ttgagcaatg ttgatgatgt ggtgagggag
      181 cttaaagata tagacttcga tatgattatt ggagggcctc cttgccaaga cttcagtcat
      241 gcaggtttaa gaattgaagg tgctagagct aacttaactc gctcattttc agaaattata
      301 aaaagaataa agccaaagtg gtttgtgatg gaaaatgtag atcgggcatt aagaagtggt
      361 gcctatttag aagctcgagg aatctttaaa gaatcagggt atgggttaac agaaattgtt
      421 ttggatgcca gtaagtgtgg tgttccgcaa aaaagaaaaa gattatttgt aattggaaag
      481 cttgacgtga gggatggttt tatccttaac gaagtgatgt gcgggatatc gaaagatagt
      541 atgacagtta gaaattatct tggtgattca ttggggattg agtattatta cagacatccg
      601 agaaattata acagaagggc tattttctct attgatgaac ctgcgccaac agtaagaggg
      661 gttaatagac cgatacctga tggatattta gggcatgcag gcgatcctgt cagtatttct
      721 gagaatgtaa ggccattaac aacctttgaa agagcaagat tgcaaacatt tcctgaagac
      781 tttaaattta aaggtgcaaa aacaaatttg gagcaaatga taggtaatgc tgttccggtt
      841 gagttagcaa aatatgttgc ggtgacaatc atggagtatg aaaaaaaaca agtaaaaggt
      901 atttatgata aagaaggctt tagggcgtgg ttgctgaatg aaaagaaact aacaaaaaga
      961 acctctagcg atattatatc tagatgttgc agaggtgttt cattttttga ttcagagggg
     1021 gtggattttt ataactgtga aatagatgaa attattatga aactggaaag gcttgaatcg
     1081 tttgtcaggc ttggggttag cctgaagtct cagttaagaa gggcttttaa attatattac
     1141 gaatattgtc gaaggtaata aatacgaatt tatgtctctc aggggagggg gaggggataa
     1201 aaagatcaaa ggatcctctt gtgatccttt ttttctgcgc gtaatctgct gcctgtaaac
     1261 aaaaaaacca ccgctaccaa cggtggtttg tttgccggaa tctagagcta ccaactcttt
     1321 cttccgaagg taactggctt ggtggagcgc agataccaaa tactgtcctt ctagtgtagc
     1381 cgcagttagg ccaccacttc aagaactctt aatatctcaa tccaccttgt ccagttacca
     1441 gtggctgctg ccagtggcgt taagtcgtgt ctttccgggt tggactcaag acgatagtta
     1501 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     1561 cgaacgacct acaccgaact gagataccaa cagcgtgagc tatgagaaag cgccacgctt
     1621 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     1681 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     1741 ctctgacttg agcgtcgatt tctgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     1801 gccagcggcg cggccttttg ttgtcctttg cctttttgct cacatgttct ttccggcctt
     1861 atcccctgat tctgtggata accgtattac cgcctttgag tgagccgact ccgctcgccg
     1921 cagccgaacg accgagcgta gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg
     1981 gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatggtgct cttttcttat
     2041 aatccgcact gatgccacat agttaagcca gtatacactc cgctatcgct acgtgactgg
     2101 ttcagggctt cgccccgaaa ccccctgacg cgccctgacg ggcttgtctg ctcccggcat
     2161 ccgctcacag gcaagctgtg accgtctccg ggagctgcat gtgtcagagg tcttcaccgt
     2221 catcaccgaa acgcgcgagg cagctgcggt aaagctcatc ggcgtggtcg tgaagagatt
     2281 cacaaatatc ggcctgttca tctgcgtcca gttcgttgag tttctccagc agcgttaatg
     2341 tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg
     2401 cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgcgagagga
     2461 tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgc tgtgagggta
     2521 aacaactggc ggtatggatg cggcgggtct gcctgggtga gccggttgcc cgttccggga
     2581 agctgccgac actggcaccg ccgttactgc gtcagctggc cgccatcggg aataacctga
     2641 atcagacagc gaggaaggta aacagcgggc agtggtcttc cggtgaccgg gttcaggtgg
     2701 tggccgcact gatggccatc gagggagagt tgcgcagctt acggcaggtc gtcagggagc
     2761 agggagcgcg ggatgattgt taaatttcat gccaggggaa aaggcggtgg cagtggtccg
     2821 gttgattacc tgctgggaag ggagcgtaac cgggaagggg caacggtgct tcagggtaat
     2881 ccggaagaag tcagggaact catcgatgcc acgccatttg cgaagaaata cacgtccggt
     2941 gttctgtcgt tcgcggagaa ggaactgccg ccgggaggac gtgaaaaagt gatggagagc
     3001 tttgagcgcg tactgatgcc cggtctcgaa aaaaatcagt acagcattct gtgggtggag
     3061 caccaggaca aggggcgtct tgagctgaat tttgtcattc cgaacatgga gctacagacc
     3121 ggaaaacgcc tccagccgta ctacgaccga gcagacaggc cgagaattga tgcctggcag
     3181 acactggtga atcaccatta cgggctgcat gaccccaacg ccccggagaa ccgccggata
     3241 ctgactctct ctgataacct gccggaaacg aaacaggcgc ttgctgagag cgtcacacgc
     3301 gggatagacg ctctttatca tgtaggggag ataaaaggcc gtcaggatgt gattcaggcg
     3361 ctcacagagg cggggcttga agtggtcagg gtgacgcgaa gcagcatcag cattgcagac
     3421 ccgaacggcg ggaagaatat caggctgaaa ggagcgtttt atgagcaatc ttttacagac
     3481 gggcgcggag ttcgagaaaa agctgaaaga gagagccgaa tctacagaga aaatgctgaa
     3541 cgacgagttc aggaagctcg gcgagtctgt cagcgaggct gtgacctcaa acgagacgaa
     3601 aatcagagac gctatagccc tgttcacagc ctcgacagag aaatcgctgg aaaaacacag
     3661 ggaaggggtg aaagaggcga tgatgcagca caggaaggac gtgttaaagc tggcagggaa
     3721 tacgggcatg atgttactgg ggatagtctt tctcctgttt accgcgagtg gcgggacgct
     3781 ctggtatctt ggcgggagga tacaggcgaa cctggaagaa atcaggaagc aggaagagac
     3841 attgcagaaa ctgaacgcga agacatgggg cgtggagttt gtgcaggacg ggagaaggaa
     3901 atttctggtc attccgcaag ggatgtcagc agaggtgacg cactatcagg ggaaggactg
     3961 gatacaactg acggagtgat acggaatgac agagctggaa acacatttgc tgaacgcctt
     4021 agagcagctg caacaggact atatgcagcg gctgaacgaa tgggagagcg ccttcgtgga
     4081 attgcagaag atgttttcgc ttacgcaacg ggacaacgcg atgctgaacg agcgggtcat
     4141 gcagttgagt cagcaggtgc agcacttgag cgagcggaca gaacgcttga gccagttata
     4201 cagcgagaac tggagataag agaggaacgg ctgatacagg agcgcgaaca tgcgttatcc
     4261 ctggaacggg agcgtcagcc ggaaatacag gaacgcatac tggatggtcc ttcgttgtcg
     4321 ctgtgatggc gaaactataa aaaatggcag gttcagtgga ttttgacgga ctaatgtagt
     4381 ctgcaccatc tggttgcata ggtattcatg cggttaaaat ttatcaggcg cgatcgcggc
     4441 agtttttcgg gtggtttgtt gccattttta cctgtctgct gccgtgatcg caccgaacgc
     4501 gttttagcgg tgtgcgtaac ggatgttatg gtaaatacaa tatgcagcca gtcttagaga
     4561 cgaaaagtcc gcccagaaga ctgacttttc agatcaacct tcagcatcta acttgtctat
     4621 cgctttttta ttgcgccgat aggtagccat tagtttacca tcgcctgatt gtaagtcacg
     4681 acctgcttgc tcagcatagg tatcgtttac agtcttagcg ccttttttat aggccttttt
     4741 aacatcatca aatccggctt ggatgacttc tttcggcttt tcttgaggta atttagccat
     4801 gctgtgaatc ctctataagg ttgattattt ttccaacaga taagacggca tcttctgtgt
     4861 cgctgatttt atgatttatc caaccgtcct ctatctttaa tttgttcact actcctaatg
     4921 taaaaacaat ctttttttca ggaaagaaaa ttgcaaaggt tctacttttc tctttccaat
     4981 cagagggcgg cgtcgaacgg aacgctacat tcctcgattt aagtatcgct ttgattttac
     5041 taattaattc ttttttttgt ttattgctga gatttttgac gaaccctttg ctgagattgt
     5101 ctgtgtaatc atcaaaagaa atatcaactg gtttgttttt ttgtttttta tcagatagat
     5161 gaacaatgtt tgacactgga tatcctgctt tttgatgaat gaatactcag aagagtagcg
     5221 gaactgtctt gttagtcaca gaggtagagt atcaattcta cgatagatgg gttcgaagat
     5281 ccggctgaga ccgttcagta cgcggtcgag atgttatgtt caatgtaatg accgtcgaaa
     5341 ttgcctttac gggcatgaag caaccaggag caatcattgg caaaggtaac tagagttcaa
     5401 tgcatggtaa tcatttttga gcttgtgatg catagagttg gttgctgttc cattgcgtct
     5461 caaactccgt caagatgctc gccgtaaaag cgagcatgtc tagtatgcct gttccgctac
     5521 tgatttttga tgggcgaatt cagggctggc gagccaccaa gaaaaattac tttatttcat
     5581 caaacaggtg agatgcagta taataaacgc tttgatcaaa gcgtctctct gggcttaatt
     5641 tattttgccc gccatttaaa attatttctt ttaatactga tggtgggagg atgggttttg
     5701 ttcttcttgg gtgaaccata taagcttttg ttatttttcc gtttagcgag aactctttat
     5761 cactttgata tccaaagttc atctcagtat aaattctatt gatgtttgtc ttgtttttgc
     5821 tcgcaatttc atacaccatt ctggcgagtc tggctgatga tctcattagt ctgctggcag
     5881 atgaattgtt atatgaggat ctatcaataa aggttcgaca caaagcaaaa tcactccaga
     5941 tgaatatgtc aaaggcattt tgatctaata ttgggctctt gccttttgtt ttccataatg
     6001 gttgcattaa aaaaggtttc tggtaatcaa gaaaattttc ttggaaagtg tttaggcaat
     6061 ttaatatatt ttctttcttt gaaagaactt cagtttcatt cccccagtgc tgtatgcttt
     6121 cgcatgtgga ttcaaatatt tttcttattt catggaattt atcgctgcaa gagtgcatta
     6181 tgccaagggc acaataagaa gtgcttgcag gtcttattac aatctctgaa ctccattcgc
     6241 tctcgttttc tttatatgtc gtgctatctg gaagtacggt taattttatt tctagtgctt
     6301 ttaaaaaact attgtttcct tttataacta gatctattcc ttttatgtca tcataagcat
     6361 actgttggta tggttcatat tttttctcaa attgaaattc aagatcacta ttttttaatg
     6421 ttgtgttaaa aacgaagtca aaagatactt ctttggcatg cacttttaaa tccttatcta
     6481 aataaagata aatcggattc attttgttgt ctctcatata acatgctaga gaggtgggaa
     6541 aagaactgtt aaattggttc tttccccaca gatcgctctc tttcctgttg gtgtttttta
     6601 aaccataaag agattctatt ttgctcactt tttgctcctg acgtcaattt attgacgtct
     6661 atggtgtgtt tctatatcat gatgtggagt gatatgataa tgagtttttt atgtt
//
