LOCUS       NZ_CP031915             6675 bp    DNA     circular CON 19-JUN-2024
DEFINITION  Escherichia coli O157:H7 strain FWSEC0004 plasmid unnamed14,
            complete sequence.
ACCESSION   NZ_CP031915
VERSION     NZ_CP031915.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN08768105
            Assembly: GCF_005037735.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli O157:H7
  ORGANISM  Escherichia coli O157:H7
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6675)
  AUTHORS   Tyson,S., Peterson,C.-L., Olson,A., Tyler,S., Cabral,J., Lynch,T.,
            Knox,N., Van Domselaar,G. and Graham,M.
  TITLE     Food and Water Consortium WGS
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6675)
  AUTHORS   Tyson,S., Peters,G.A., Melnychuk,K., Laminman,V. and Kaplen,B.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-AUG-2018) National Microbiology Lab, Public Health
            Agency of Canada, 1015 Arlington Street, Winnipeg, MB R3E3R2, Canada
REFERENCE   3  (bases 1 to 6675)
  AUTHORS   Cabral,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2017) National Microbiology Laboratory, Public
            Health Agency of Canada, 1015 Arlington St, Winnipeg, Manitoba R3E
            3R2, Canada
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: AUG-2018
            Assembly Method                   :: Unicycler;Canu v. 0.4.4.0;1.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 130.71x;172.42x
            Sequencing Technology :: Illumina MiSeq;Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_005037735.1-RS_2024_06_19
            Annotation Date                   :: 06/19/2024 09:50:19
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,482
            CDSs (total)                      :: 5,352
            Genes (coding)                    :: 5,070
            CDSs (with protein)               :: 5,070
            Genes (RNA)                       :: 130
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 100
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 107 of 282
            Pseudo Genes (incomplete)         :: 161 of 282
            Pseudo Genes (internal stop)      :: 66 of 282
            Pseudo Genes (multiple problems)  :: 42 of 282
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP031915.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6675
                     /organism="Escherichia coli O157:H7"
                     /mol_type="genomic DNA"
                     /strain="FWSEC0004"
                     /serotype="O157:H7"
                     /isolation_source="Clinical: Human (Homo sapiens)"
                     /host="Homo sapiens"
                     /db_xref="taxon:83334"
                     /plasmid="unnamed14"
                     /geo_loc_name="Canada:Ontario,Toronto"
                     /collection_date="1987"
                     /collected_by="National Microbiology Laboratory: Public
                     Health Agency of Canada"
     gene            25..348
                     /locus_tag="CAM50_RS27930"
                     /old_locus_tag="CAM50_0029230"
     CDS             25..348
                     /locus_tag="CAM50_RS27930"
                     /old_locus_tag="CAM50_0029230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_001444404.1"
                     /translation="MLTIRVTDEEHARLLARCEGKQLASWMRKVCLGAPPSKTSGLPTL
                     APPLLRQFASVGNNLNQIARKINSGQWSGHDRVHVVAALMAIGRELSELRDEVRKQGER
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            557..>991
                     /locus_tag="CAM50_RS28670"
                     /pseudo=""
     CDS             557..>991
                     /locus_tag="CAM50_RS28670"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            1044..1586
                     /locus_tag="CAM50_RS27940"
     CDS             1044..1586
                     /locus_tag="CAM50_RS27940"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_235777954.1"
                     /translation="MGTDFEKKLKERAVSTENMLNSEFRKLEASVDRELNLNGQKIRDA
                     ISAHTAAVKEQLEMLNSAVNAQFSATENELARRQEELLWKLVKGRILYPSLTALCVTVG
                     IFLASWGLIQWQESRIAANILAIRDQEETLAKLREKTWGVTYHEGRNGKFLVLPSGVKG
                     ENNWTVEKKNAVRLVRE"
                     /name="MbeB family mobilization protein"
     gene            1593..1826
                     /locus_tag="CAM50_RS27945"
     CDS             1593..1826
                     /locus_tag="CAM50_RS27945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137429.1"
                     /translation="MTELEMHLLNALEQLQQDYTQRLKDWESAFTDLQHMFELTRQENG
                     QLREQCDDLSQQVQRLAGQVDRLSRLFITNSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(2316..2561)
                     /locus_tag="CAM50_RS27955"
                     /old_locus_tag="CAM50_0029250"
     CDS             complement(2316..2561)
                     /locus_tag="CAM50_RS27955"
                     /old_locus_tag="CAM50_0029250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000820629.1"
                     /translation="MKRHRLTEAEERRLAEAIAAEARAMGLTKKDKMPQETKRRLRARI
                     LKAILDERTASKNREATLTAGQDPDDTFTWQPLRRR"
                     /name="hypothetical protein"
     gene            2825..4918
                     /gene="cda"
                     /locus_tag="CAM50_RS27960"
                     /old_locus_tag="CAM50_0029255"
     CDS             2825..4918
                     /gene="cda"
                     /locus_tag="CAM50_RS27960"
                     /old_locus_tag="CAM50_0029255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-D"
                     /protein_id="WP_001286654.1"
                     /translation="MSDYEGSGPTEGIDYGHSMVVWPSTGLISGGDVKPGGSSGIAPSM
                     PPGWGDYSPQGIALVQSVLFPGIIRRIILDKELEEGDWSGWSVSVHSPWGNEKVSAART
                     VLENGLRGGLPEPSRPAAVSFARLEPASGNEQKIIRLMVTQQLEQVTDIPASQLPAAGN
                     NVPVKYRLMDLMQNGTQYMAIIGGIPMTVPVVDAVPVPDRSRPGTNIKDVYSAPVSPNL
                     PDLVLSVGQMNTPVLSNPEIQEEGVIAETGNYVEAGYTMSSNNHDVIVRFPDGSGVSPL
                     YISTVEILDSNGLSQRQEAENKAKDDFRVKKEEAVARAEAEKAKAELFSKAGVNQPPVY
                     TQEMMERANSVMNEQGALVLNNTASSVQLAMTGTGVWTAAGDIAGNISKFFSNALEKVT
                     IPEVSPLLMRISLGALWFHSEEAGAGSDIVPGRNLEAMFSLSAQMLAGQGVVIEPGATS
                     VNLPVRGQLINSNGQLALDLLKTGNESIPAAVPVLNAVRDTATGLDKITLPAVVGAPSR
                     TILVNPVPQPSVPTDTGNHQPVPVTPVHTGTEVKPVEMPVTTITPVSDVGGLRDFIYWR
                     PDAAGTGVEAVYVMLNDPLDSGRFSRKQLDKKYKHAGDFGISDTKKNRETLTKFRDAIE
                     EHLSDKDTVEKGTYRREKGSKVYFNPNTMNVVIIKSNGEFLSGWKINPDADNGRIYLET
                     GEL"
                     /name="cda"
     gene            4915..5178
                     /locus_tag="CAM50_RS27965"
                     /old_locus_tag="CAM50_0029260"
     CDS             4915..5178
                     /locus_tag="CAM50_RS27965"
                     /old_locus_tag="CAM50_0029260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032488334.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin immunity domain-containing protein"
                     /protein_id="WP_001038407.1"
                     /translation="MNKMAMIDLAKLFLASKITAIEFSERICVERRRLYGVKDLSPNIL
                     NCGEELFMAAERFEPDADRADYEIDDNGLKVEVRSILEKFKL"
                     /name="colicin immunity domain-containing protein"
     gene            5248..5394
                     /locus_tag="CAM50_RS27970"
                     /old_locus_tag="CAM50_0029265"
     CDS             5248..5394
                     /locus_tag="CAM50_RS27970"
                     /old_locus_tag="CAM50_0029265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711480.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_000726755.1"
                     /translation="MKKKIGGSMILVLAVLCLTACQANYVRDVQGGTIAPSSSSKLIGV
                     AVQ"
                     /name="colicin release lysis protein"
     gene            6101..6289
                     /locus_tag="CAM50_RS27985"
                     /old_locus_tag="CAM50_0029280"
     CDS             6101..6289
                     /locus_tag="CAM50_RS27985"
                     /old_locus_tag="CAM50_0029280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001479923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_024177148.1"
                     /translation="MDNTITAFEWADTARRSRTTERSESVSEEAEKRLDEHFLLTHLCG
                     ISHPAWCTFHTILTDAT"
                     /name="hypothetical protein"
     gene            complement(6441..6632)
                     /locus_tag="CAM50_RS27990"
                     /old_locus_tag="CAM50_0029285"
     CDS             complement(6441..6632)
                     /locus_tag="CAM50_RS27990"
                     /old_locus_tag="CAM50_0029285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165980.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADDQADICEALHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     BGC             1..6675
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          6270..6356
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.864"
                     /coverage="99.0"
                     /evalue="1.15e-36"
     Typing          5624..5808
                     /name="rep_cluster_2401"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.774"
                     /coverage="98.0"
                     /evalue="2.18e-82"
     Typing          339..1933
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.06"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          6439..6641
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="89.706"
                     /coverage="100.0"
                     /evalue="2.05e-13"
ORIGIN
        1 atgataccgg tgaaacgcga aaggatgctc acgatacggg tcacggatga ggaacatgcg
       61 cggctgctgg cacgttgtga gggaaaacaa cttgcgtcat ggatgcgtaa ggtctgcctg
      121 ggggcaccgc catcgaaaac gtcaggtctt ccgacactgg caccgccgct gttgcgccag
      181 ttcgcttcgg tcggcaataa cctgaatcag atagcccgga agataaacag tggacagtgg
      241 tcggggcatg accgggttca tgtggtggct gcactgatgg ccatcggacg tgaactgagt
      301 gagctgaggg atgaggtccg taaacaggga gaacgggatg atagttaagt ttcatgccag
      361 gggaaaaggc ggtggcagtg gtccggttga ttacctgctg ggccgggagc gtaaccggga
      421 aggggcgaga gttctgcggg gtgctcccga agaggtgcgg gaactcatcg atgcgacgcc
      481 gtttgcgaaa aaatacacct cgggggttct gtcttttgca gagcagaccc tgccaccggg
      541 agagcgtgaa agggtgatgg agagctttga gcgggtactg atgcccggtc ttgaaaaaaa
      601 tcagtacagc atcctgtggg tggagcacca ggacaagggg cgtcttgagc tgaattttgt
      661 catcccgaat atggagctgg cgagcggaaa acggctccag ccctattacg accgggcgga
      721 ccgtccgcga atcaatgcct ggcagacact ggtgaatcac cattacgggc tgcacgaccc
      781 gaacgcaccg gagaacaggc ggacacttgt caccccgaat aatctgccga aagcgaaaca
      841 ggaggccgca gaagcgatta cgaggggact ggaggctctt taccatgccg gagcgctaaa
      901 aacgcgtcag gatgtcactg aggcgcttac agcggcaggt tttgaggttg tgaggacaac
      961 caggagcagt atcagtatcg ccgacccgga gggtgggcgt aatctcaggc tgaaaggggc
     1021 gctgtatgag caatctttta caaatgggga cagacttcga gaagaaactg aaagagcgtg
     1081 ccgtctctac agagaacatg ctgaacagcg agttcaggaa gctcgaagcg tctgtagaca
     1141 gggaactgaa cttaaacggg cagaaaatca gagacgctat cagcgcacac acagcagcgg
     1201 tgaaagaaca actggagatg ctgaacagtg ccgtgaacgc gcagttcagc gccacagaga
     1261 acgaacttgc ccggcggcag gaagagctgc tctggaagct ggtcaaaggg cggatactgt
     1321 acccgtcgct gacggcgctt tgcgtcacgg tcgggatttt tctggcgtca tgggggctga
     1381 ttcagtggca ggaaagccgt attgcggcga acatcctggc tatcagggac caggaagaga
     1441 cgctggcgaa actccgggaa aaaacatggg gtgtgacgta tcacgagggc aggaacggga
     1501 aatttctggt gctgccgtcc ggggtgaagg gggaaaacaa ctggacggtg gagaagaaga
     1561 acgcagtaag actggtcagg gagtaagtga gtatgacaga gctggaaatg cacttactga
     1621 acgccttaga gcaattgcag caggactata cgcagcggct gaaagactgg gagagcgcct
     1681 tcacggattt gcagcatatg ttcgagctga cacgacagga gaacgggcaa ctgagggaac
     1741 agtgcgacga cttgagtcag caggtgcaga gattagccgg gcaggtggac cgcttgagtc
     1801 ggttatttat cacgaacagc agataaggga tgaacaggaa aggcaggcac gcctggaacg
     1861 ggagcgtgcg cggtcctggc atgaagagca ggaacgggaa gcacaggtat ggcgtggccc
     1921 gtcaatgggg atgtgacggt gagttgttcc gggaggttgc cagatggtac cggaacgcgt
     1981 taaaaacgca tccgggcgcg attctggcgt gtcgtttttg ttgctgtgtt ttgcctcttt
     2041 gccagatcgc gctcattcct cagcctcatc ccgcgatgtc tgcgtatgtc atcaactgaa
     2101 aagatggaaa aaaaacagtg tcatgaggtt tacgcaggca aaaccagcgt tattcacatg
     2161 gctgaatagc tatgcatacg gttaaaattt atcaggtgcg atcgcggcag cttttcgggt
     2221 ggtttgttgc catttttacc tgtcagcagc cgtgatcgcg ctgaacgcat ttcagcggtg
     2281 cgtacaataa ggggttatgt taaataaccc ttagcttagc ggcggcgcag tggttgccag
     2341 gtgaaggtgt catccgggtc ctgtccggca gtcagtgtgg cttctcgatt tttgctggcc
     2401 gttcgttcat caagaatggc tttaagtatc cgtgcccgga ggcgtcgctt tgtttcctgc
     2461 ggcattttgt cctttttcgt cagccccatg gcacgagctt cagcggcaat ggcttctgcc
     2521 agtcggcgct cttcggcttc ggtcagtcta tggcgtttca tggtggtctc cgggtgtttc
     2581 tctggtgtca taacagttta actgttggct ctctccttat gctaatcagc cttctttcat
     2641 gcctgttgcc attgagttta agaatgattg gttgtttttc ttgagcgttt tcacaaaaac
     2701 gtaaatccag tgggttgaca ggatttacga ggtgacgtaa ctttttactg tacataaaac
     2761 cagtggtttt atgtacagtt tgttgttttt aacgcattgt tttaaaagtc aaagaggtgt
     2821 ttttatgagt gattacgaag gtagtggtcc gacagaaggt attgattatg ggcactcgat
     2881 ggtcgtgtgg ccgtcaacag gactgatttc gggtggtgat gtgaaaccag gaggctcatc
     2941 aggtatcgct ccatccatgc ctccgggatg gggggattac agcccacagg gtatcgcact
     3001 tgtacaaagt gttctttttc cgggaattat tcgccggatt attctggata aggaacttga
     3061 agagggagac tggtcgggat ggtctgtcag tgtgcatagc ccttggggaa acgagaaagt
     3121 ttccgctgca cgaacagttc ttgagaatgg tttacgtggt ggtttgccag aaccgtctcg
     3181 cccggctgct gtttcttttg cccgtctgga gcctgcttcc ggaaatgagc aaaaaattat
     3241 tcgtcttatg gttacacagc aactggaaca ggtaacggat attccggcca gccagttacc
     3301 agcagcgggt aataatgtac cggtgaaata tcgtctgatg gaccttatgc agaacggtac
     3361 gcaatatatg gctattatcg gaggtattcc gatgacagtg ccggtagttg atgccgttcc
     3421 agttccggac cggagtcgtc cgggaacaaa tattaaagat gtttacagtg cccctgtatc
     3481 accaaatcta ccggacctgg tattaagtgt gggtcagatg aatactccgg ttctgtctaa
     3541 tcccgaaatt caggaagaag gagttattgc tgagacaggt aattatgttg aggctggtta
     3601 tacgatgtcc agtaataatc atgatgtcat tgttcgtttt cctgatggca gtggtgtttc
     3661 tccgttatat atttcaactg tagagattct ggacagtaat ggtctgagtc agcgtcagga
     3721 agccgaaaat aaagcaaagg atgatttcag agtcaagaaa gaagaagcgg tagcccgggc
     3781 tgaagctgaa aaagcaaaag ccgagttatt tagtaaagca ggtgtgaacc agcctcctgt
     3841 atatacacag gaaatgatgg aaagggctaa ttcagtaatg aatgagcagg gagctcttgt
     3901 tctgaataac actgccagtt ctgtacaact ggcgatgacc ggaactggtg tctggactgc
     3961 tgctggtgat attgcaggca acataagcaa gtttttcagt aatgctctgg agaaagtaac
     4021 catacccgaa gtgagtcccc tgcttatgcg gatttctctt ggtgctctgt ggtttcattc
     4081 agaagaagct ggagcaggaa gtgatatcgt gccggggcga aatctggagg cgatgttttc
     4141 gctgagtgct cagatgttgg ctggacaggg cgtggtcatt gaacctggtg cgacgagtgt
     4201 aaatctgcct gttcgtggac aattgataaa cagtaacggg caattagctc tggatttact
     4261 gaaaacaggg aatgaaagta tcccagctgc ggttcctgtt cttaatgctg tccgtgatac
     4321 agcaacagga ctggataaaa tcacgttacc agcagtagta ggcgcacctt cccggacgat
     4381 tctggttaat ccggtaccac aaccttcggt gccaacagat acaggtaatc atcaacccgt
     4441 tccggttaca ccagtgcata caggaacgga agtaaaaccg gtcgaaatgc cagtaacgac
     4501 gattactcct gtttctgatg ttggtggact acgagatttt atttactggc gtcctgatgc
     4561 tgctgggact ggtgttgaag ctgtttatgt gatgcttaat gatcctctgg attcagggcg
     4621 attttcgcgt aaacaacttg ataaaaaata taaacatgct ggtgattttg gtattagtga
     4681 tacaaaaaag aatcgtgaaa ctcttactaa attcagggat gctattgagg agcatttatc
     4741 ggataaggat acagtagaga aaggaacata ccgaagagaa aaaggttcaa aagtttattt
     4801 taatcctaat acgatgaatg tggttataat taagtcaaat ggtgagttct tatctgggtg
     4861 gaaaataaat ccagatgcgg ataatggtcg aatttattta gagacaggtg aactatgaac
     4921 aagatggcaa tgatcgattt ggcgaaatta tttttagctt cgaaaattac agcaattgag
     4981 ttttcagagc gaatttgtgt tgaacggaga agattgtatg gtgttaagga tttgtctccg
     5041 aatatattaa attgtgggga agagttgttt atggctgctg agcgatttga gcctgatgca
     5101 gatagggctg attatgaaat tgatgataat ggacttaagg ttgaggtccg atctatcttg
     5161 gaaaaattta aattataatc atgagtgatt ttaatatctg gcatttaagt ataaatgtca
     5221 gatattcact catgacaagg agatgtcatg aaaaaaaaga tagggggatc tatgattctc
     5281 gttcttgctg ttttgtgtct gactgcttgc caagctaact atgtaaggga tgttcagggt
     5341 ggaacaattg ctccatcatc atcttcgaaa ctaatcgggg tggcggttca gtgaaaaata
     5401 ttaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcctg taaacgaaaa
     5461 aacccgcctt gtggcgggtc cttccgtccg atcagaagtc ttcgacctct tctaatcggc
     5521 ggtggtaact ggcttggagg agcacagtca ccaaaactgt cttttcagtt tagccttaac
     5581 tggttcataa cttcaagagc aactcctcta aatcagttac caatggctgc tgccagtggc
     5641 gttaagtcgt gtcttttccg ggttggcccc aagagaacag ttaccggaga aggtgcagca
     5701 gtcgggctga acggggggtt cctgcataca gcccagcccg gagcgaactg cctaagtcgc
     5761 cccgagatac cagaagacgt gagctatgag aaagcgccac gtgtcccgca agggagaaag
     5821 gcggaaaagg tatccggtaa tcgatatggc gggaacgtgg gggcgcagga gggagcgatt
     5881 caccggaaac ggtgatgatc tttaagccac gtcgggtttc gccaccgctg gcggatttca
     5941 tggttgagcc tcacggctcc cacagatgca ccgaaaaagt gtctgttttg tgaagtccgt
     6001 caggggggcg gagcctatgg aaaaagcgcc tccggcgcgg cctttttcgg ggttttcgtt
     6061 tcttgctcac atgttctttc cggcgttatc ccctgattct gtggataaca ctatcaccgc
     6121 ttttgagtgg gcagataccg ctcgccgcag ccgaacgacc gagcgtagcg agtcagtgag
     6181 cgaggaagcg gaaaagcgcc tggacgagca ttttctcctt acgcatctgt gcggcatttc
     6241 acacccggca tggtgcactt ttcataccat cctcactgat gccacatgat taagccagta
     6301 tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gctaaaaccc
     6361 gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg ctcacaggca agctgtgacc
     6421 gtctccggga gctgcatgtg tcagaggttt tcaccgtcat ccccgaagcg tgcgaggcag
     6481 ctgcggtaaa gctcgtcagc gtggtcgtga agcgcttcgc agatgtcagc ctggtcgtcg
     6541 gcgtccagtt cattcagttt ttccagcagg gtcagcgtct ggcttctgat gaagcgggcc
     6601 atgttaaggg cggttttttc ctgtttagtc acagttctgt ttctccatgt gtggggagtc
     6661 tgtcggaggt tttta
//
