LOCUS       NZ_CP031905             1549 bp    DNA     circular CON 19-JUN-2024
DEFINITION  Escherichia coli O104:H4 strain FWSEC0009 plasmid unnamed20,
            complete sequence.
ACCESSION   NZ_CP031905
VERSION     NZ_CP031905.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN08768110
            Assembly: GCF_005014075.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli O104:H4
  ORGANISM  Escherichia coli O104:H4
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1549)
  AUTHORS   Tyson,S., Peterson,C.-L., Olson,A., Tyler,S., Cabral,J., Lynch,T.,
            Knox,N., Van Domselaar,G. and Graham,M.
  TITLE     Food and Water Consortium WGS
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1549)
  AUTHORS   Tyson,S., Peters,G.A., Melnychuk,K., Laminman,V. and Kaplen,B.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-AUG-2018) National Microbiology Lab, Public Health
            Agency of Canada, 1015 Arlington Street, Winnipeg, MB R3E3R2, Canada
REFERENCE   3  (bases 1 to 1549)
  AUTHORS   Cabral,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2017) National Microbiology Laboratory, Public
            Health Agency of Canada, 1015 Arlington St, Winnipeg, Manitoba R3E
            3R2, Canada
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: AUG-2018
            Assembly Method                   :: Unicycler;Canu v. 0.4.4.0;1.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 65.55x;110.27x
            Sequencing Technology :: Illumina MiSeq;Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_005014075.1-RS_2024_06_19
            Annotation Date                   :: 06/19/2024 07:24:17
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,389
            CDSs (total)                      :: 5,263
            Genes (coding)                    :: 4,984
            CDSs (with protein)               :: 4,984
            Genes (RNA)                       :: 126
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 95
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 279
            CDSs (without protein)            :: 279
            Pseudo Genes (ambiguous residues) :: 0 of 279
            Pseudo Genes (frameshifted)       :: 108 of 279
            Pseudo Genes (incomplete)         :: 186 of 279
            Pseudo Genes (internal stop)      :: 58 of 279
            Pseudo Genes (multiple problems)  :: 64 of 279
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP031905.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1549
                     /organism="Escherichia coli O104:H4"
                     /mol_type="genomic DNA"
                     /strain="FWSEC0009"
                     /serotype="O104:H4"
                     /isolation_source="Clinical: Human (Homo sapiens)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1038927"
                     /plasmid="unnamed20"
                     /geo_loc_name="Germany"
                     /collection_date="2011"
                     /collected_by="Laboratory for Foodborne Zoonoses: Public
                     Health Agency of Canada"
     gene            1..477
                     /locus_tag="C8200_RS27495"
                     /old_locus_tag="C8200_28890"
                     /old_locus_tag="CCU05_RS27510"
     CDS             1..477
                     /locus_tag="C8200_RS27495"
                     /old_locus_tag="C8200_28890"
                     /old_locus_tag="CCU05_RS27510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469298.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_000469298.1"
                     /translation="MFIDSEKRLKQLSDEAKKNTEDLEEAKKNSRFTQVSPKGWERVRE
                     LLKDSQGISALKLYSFLAEHIDPTCGAVVADQQFLAEKLGVSRSTIIRWLNYLESKNAL
                     VRIPVAGKVCAYALDPHEVWKGYNTTKNHAAFVTKTLVNKDGDIQRRIMAMFSN"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(922..1047)
                     /locus_tag="C8200_RS27505"
                     /old_locus_tag="C8200_28900"
                     /old_locus_tag="CCU05_RS27520"
     CDS             complement(922..1047)
                     /locus_tag="C8200_RS27505"
                     /old_locus_tag="C8200_28900"
                     /old_locus_tag="CCU05_RS27520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001431667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001431667.1"
                     /translation="MWLPVLFRFLVDSLATLGHRLAASGITSRMLSVIPVLFFWG"
                     /name="hypothetical protein"
     Typing          1069..1167
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.22e-45"
     Typing          complement(1363..1471)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="99.091"
                     /coverage="100.0"
                     /evalue="9.09e-49"
     Typing          complement(709..969)
                     /name="Col(MG828)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="91.985"
                     /coverage="100.0"
                     /evalue="1.85e-99"
ORIGIN
        1 atgtttattg attcagaaaa acgactgaaa caactttcag atgaggcaaa gaaaaacacc
       61 gaggatctcg aagaagcaaa gaaaaattca aggtttacac aggtatcccc aaaaggttgg
      121 gaacgtgttc gagagctgct gaaggatagc caaggcatat cagcactgaa gctgtactca
      181 tttttagcgg agcatatcga tcctacgtgt ggcgctgtcg ttgcggatca gcaatttcta
      241 gctgaaaaac ttggagttag cagaagcaca attattcggt ggctcaatta cttagaatca
      301 aaaaatgcat tagttagaat ccccgttgct ggtaaggttt gtgcgtatgc cctcgatcca
      361 catgaagtct ggaagggata caacactacg aaaaaccatg cagcgtttgt cactaaaaca
      421 ctggtcaaca aagacggtga tattcagcgc cgaatcatgg ccatgttttc aaattgagct
      481 agcggcaggc ggacaatcag gggctacgtg ttaacgttct gaccatgatt gtctatcctg
      541 cattgctctt ttgccgcctc aaaatccttt gcgtgttttt gctccccgtt ctccaaaaaa
      601 aaccgagccg ccacggttcc ggccgcgcct tgagcgctac cggaaaccgt ctggggagaa
      661 ccccagaccc ccatcgctgc aacaccgacg gtctacgacc acctaccaac cgcatggctg
      721 cctgttccgt gcaaatgggc tttgcagggg gctggcgcgg ttcctggcga cctgaaagcc
      781 cctggttagg ggtatcggcg cgcttgacct cctgagagcc tctgtaagcg tttttcgcgc
      841 cttccatgcc ctggcgggca tctaaaaccc gtttcgtgcg tctgagagct tttgagcgcg
      901 tttgaggggc tatctggcga ctcatcccca aaagaacaac accgggatca ccgacaacat
      961 gcgagaggtg atcccgctcg ccgcaagtcg atgaccgagc gtagcgagcg aatcgacgag
     1021 gaagcggaag agaactggaa gccacattga gcacttacgc actgatgcgg ggtgtcgggg
     1081 tgaagccctg accaagtggt aatcgtatcg gcgtgcatgc gcggttatac gattacacat
     1141 cctgtcccga tttctgaggc gttttaacgg tgaacggacg gcaaaaaaac gatggagctg
     1201 ttgaacgtga aaaccacgaa atctggcaaa aatcacggct ggcgaggctt gcataggagt
     1261 aagccagtat acactccgct atcgctacgt gactgggtca gggcttcgcc ccgacacccc
     1321 caaagggcgt tgcgttgcac gcaacaccct tgcctagaat agatctttta cgccgatttg
     1381 taagtggttg tttttttagt gtgtttttta tttacctgtc gcatccagga tgacacaacg
     1441 ttgtcgcatc ctggatgcga caacacgcaa caaggtgttg catagatgtg tcatgatggc
     1501 ttagatttga accaacgtta gtgcatggga ttttcagagg gaaaaaatc
//
