LOCUS       NZ_CP031901             7117 bp    DNA     circular CON 19-JUN-2024
DEFINITION  Escherichia coli O113:H21 strain FWSEC0010 plasmid unnamed3,
            complete sequence.
ACCESSION   NZ_CP031901
VERSION     NZ_CP031901.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN08768111
            Assembly: GCF_005014055.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli O113:H21
  ORGANISM  Escherichia coli O113:H21
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7117)
  AUTHORS   Tyson,S., Peterson,C.-L., Olson,A., Tyler,S., Cabral,J., Lynch,T.,
            Knox,N., Van Domselaar,G. and Graham,M.
  TITLE     Food and Water Consortium WGS
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7117)
  AUTHORS   Tyson,S., Peters,G.A., Melnychuk,K., Laminman,V. and Kaplen,B.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-AUG-2018) National Microbiology Lab, Public Health
            Agency of Canada, 1015 Arlington Street, Winnipeg, MB R3E3R2, Canada
REFERENCE   3  (bases 1 to 7117)
  AUTHORS   Cabral,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2017) National Microbiology Laboratory, Public
            Health Agency of Canada, 1015 Arlington St, Winnipeg, Manitoba R3E
            3R2, Canada
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: AUG-2018
            Assembly Method                   :: Unicycler;Canu v. 0.4.4.0;1.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 94.45x;51.16x
            Sequencing Technology :: Illumina MiSeq;Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_005014055.1-RS_2024_06_19
            Annotation Date                   :: 06/19/2024 07:24:10
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,035
            CDSs (total)                      :: 4,909
            Genes (coding)                    :: 4,739
            CDSs (with protein)               :: 4,739
            Genes (RNA)                       :: 126
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 94
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 170
            CDSs (without protein)            :: 170
            Pseudo Genes (ambiguous residues) :: 0 of 170
            Pseudo Genes (frameshifted)       :: 76 of 170
            Pseudo Genes (incomplete)         :: 93 of 170
            Pseudo Genes (internal stop)      :: 41 of 170
            Pseudo Genes (multiple problems)  :: 35 of 170
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP031901.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..7117
                     /organism="Escherichia coli O113:H21"
                     /mol_type="genomic DNA"
                     /strain="FWSEC0010"
                     /serotype="O113:H21"
                     /isolation_source="Clinical: Human (Homo sapiens)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1078021"
                     /plasmid="unnamed3"
                     /geo_loc_name="Canada"
                     /collection_date="1991"
                     /collected_by="Laboratory for Foodborne Zoonoses: Public
                     Health Agency of Canada"
     gene            1..2220
                     /gene="mobQ"
                     /locus_tag="C8201_RS25430"
                     /old_locus_tag="C8201_26820"
                     /old_locus_tag="CCU06_RS25445"
     CDS             1..2220
                     /gene="mobQ"
                     /locus_tag="C8201_RS25430"
                     /old_locus_tag="C8201_26820"
                     /old_locus_tag="CCU06_RS25445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_052904183.1"
                     /translation="MAIYHLSMKIISRKNGYSAVASAAYRSGSVIPDDRTGLIHDYTRK
                     RGVDDAVILTPANAPSWCGDRSVLWNAVEKAEQRRNSQLAREIELAIPREISREAARET
                     VLAFVRENFVSRGMIADVAFHHMDRTNPHAHIMLTTRAIGETGFAGKVRDWNDRALAET
                     WRASWADHANRALANAGYQEEIDHRSYERQGLEKAPGLHLGKAACAMEKRGMETERGEQ
                     NRLINSLNLEIQVSRTQLALRTVQETQRKRELSDAARRAAEALNLTIPAANASADTLRE
                     FIATLPQECGNAWEMTPEFLAMSGKVNDIEREGNALLKEQAILEKEMTGLKKARPVASL
                     LSEIPLMTWAEPEYRKRQLRFWKLGKQIESLRRTYRAVKERDIPARRQAFETQWNTWIA
                     PGMAELKEKLSAREAERRREEAEAEARRKEQEHEARLKRHDNHRLSRETALVGVITDLG
                     RAREPGTGRITRYMMLSNRAGEFTVWGDELAHYPQSVHDPVNVYLSPGGAVMVSDIREG
                     MPESHETMARPERVRMYSGATVRHVLEQMRQGWPSYGFPALPHHWPDNFYFSDDRRPVA
                     SPLPSAQRVDITADAVPEQLMPVVFSREGNSRTLNLLLCKGPEEMLVGFVRREGGFCPV
                     LVLPSPDYSHLIVSTITENGVCLAGYGEAINHDEDTPYPPEPHLMQFRLKGHHDRLLAA
                     VHKPEEMPDYLFRQLGFNQTWHEWKRDEQHRQQQRRPGHFRGMSM"
                     /name="mobQ"
     gene            2453..4108
                     /locus_tag="C8201_RS25435"
                     /old_locus_tag="C8201_26825"
                     /old_locus_tag="CCU06_RS25450"
     CDS             2453..4108
                     /locus_tag="C8201_RS25435"
                     /old_locus_tag="C8201_26825"
                     /old_locus_tag="CCU06_RS25450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_000012964.1"
                     /translation="MSGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNP
                     WGGGSGSGIHWGGGSGHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLA
                     VSISAGALSAAIADIMAALKGPFKFGLWGVALYGVLPSQIAKDDPNMMSKIVTSLPADD
                     ITESPVSSLPLDKATVNVNVRVVDDVKDERQNISVVSGVPMSVPVVDAKPTERPGVFTA
                     SIPGAPVLNISVNNSTPAVQTLSPGVTNNTDKDVRPAGFTQGGNTRDAVIRFPKDSGHN
                     AVYVSVSDVLSPDQVKQRQDEENRRQQEWDATHPVEAAERNYERARAELNQANEDVARN
                     QERQAKAVQVYNSRKSELDAANKTLADAIAEIKQFNRFAHDPMAGGHRMWQMAGLKAQR
                     AQTDVNNKQAAFDAAAKEKSDADAALSSAMESRKKKEDKKRSAENNLNDEKNKPRKGFK
                     DYGHDYHPAPKTENIKGLGDLKPGIPKTPKQNGGGKRKRWTGDKGRKIYEWDSQHGELE
                     GYRASDGQHLGSFDPKTGNQLKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            4118..4375
                     /locus_tag="C8201_RS25440"
                     /old_locus_tag="C8201_26830"
                     /old_locus_tag="CCU06_RS25455"
     CDS             4118..4375
                     /locus_tag="C8201_RS25440"
                     /old_locus_tag="C8201_26830"
                     /old_locus_tag="CCU06_RS25455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523346.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_000523346.1"
                     /translation="MGLKLDLTWFDKSTEDFKGEEYSKDFGDDGSVMESLGVPFKDNVN
                     NGCFDVIAEWVPLLQPYFNHQIDISDNEYFVSFDYRDGDW"
                     /name="colicin E3-like toxin immunity protein"
     gene            4524..4799
                     /locus_tag="C8201_RS25445"
                     /old_locus_tag="C8201_26835"
                     /old_locus_tag="CCU06_RS25460"
     CDS             4524..4799
                     /locus_tag="C8201_RS25445"
                     /old_locus_tag="C8201_26835"
                     /old_locus_tag="CCU06_RS25460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="WP_050544378.1"
                     /translation="MLRRGEGGAKKSIGDYTETEFKKIIEDIINCEGDEKKQDDNLEYF
                     INVTEHPSGSDLIYYPEGNNDGSPEGVIKEIKEWRAANGKSGFKQG"
                     /name="bacteriocin immunity protein"
     gene            4861..5004
                     /locus_tag="C8201_RS25450"
                     /old_locus_tag="C8201_26840"
                     /old_locus_tag="CCU06_RS25465"
     CDS             4861..5004
                     /locus_tag="C8201_RS25450"
                     /old_locus_tag="C8201_26840"
                     /old_locus_tag="CCU06_RS25465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724569.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_000724383.1"
                     /translation="MKKITGIILLLLAVIILSACQANYIRDVQGGTVSPSSTAEVTGLA
                     TQ"
                     /name="colicin release lysis protein"
     gene            5533..6447
                     /locus_tag="C8201_RS25455"
                     /old_locus_tag="C8201_26845"
                     /old_locus_tag="CCU06_RS25470"
     CDS             5533..6447
                     /locus_tag="C8201_RS25455"
                     /old_locus_tag="C8201_26845"
                     /old_locus_tag="CCU06_RS25470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001277132.1"
                     /translation="MSAVLQRFREKLPHKPYCTNDFAYGVRILPKNIAILARFIQQNQP
                     HALYWLPFDVDRTGASIDWSDRNCPAPNITVKNPRNGHAHLLYALALPVRTAPDASASA
                     LRYAAAIERALCEKLGADVNYSGLICKNPCHPEWQEVEWREEPYTLDELADYLDLSASA
                     RRSVDKNYGLGRNCYLFEKGRKWAYRAIRQGWPAFSQWLDAVIQRVEMYNASLPVPLSP
                     PECRAIGKSIAKYTHRNFTPETFAQYVADTHTPEIQATRGRKGGSKSKRGTVATSARTL
                     KPWEKLGISRAWYYQLKKRGLVE"
                     /name="replication initiation protein"
     gene            complement(6664..6939)
                     /locus_tag="C8201_RS25460"
                     /old_locus_tag="C8201_26850"
                     /old_locus_tag="CCU06_RS25475"
     CDS             complement(6664..6939)
                     /locus_tag="C8201_RS25460"
                     /old_locus_tag="C8201_26850"
                     /old_locus_tag="CCU06_RS25475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000091752.1"
                     /translation="MSRTLEQKIAEAEARLQRLKAKSRSLDTAQKVIVGAAMLARVRRP
                     EEAQLRAFLLQFLRKEVTRQADVNRLQPLINELEKLPRPPAKPQNH"
                     /name="hypothetical protein"
     BGC             1..7117
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(2..359)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="99.443"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(6944..7117)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="99.429"
                     /coverage="100.0"
                     /evalue="1.19e-84"
     Typing          5534..6447
                     /name="rep_cluster_2134"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.891"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          2..2217
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.459"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atggcgattt atcatctcag catgaaaatc atttcgcgaa aaaacggcta cagtgctgtt
       61 gcttctgctg cctaccgttc cggctctgtc atacccgatg accgtaccgg attaatccac
      121 gattacaccc gtaaacgcgg cgttgatgat gcggtcattc tcacccctgc gaatgcaccg
      181 tcctggtgtg gtgaccgttc cgttctctgg aatgcggtcg agaaagccga acagcgccgg
      241 aactcccagc tggcaaggga gattgaactc gccattcccc gtgagatttc ccgcgaggcc
      301 gcacgggaga ccgttctcgc ttttgtccgg gaaaactttg tcagtcgggg catgattgcc
      361 gatgtggcgt tccatcacat ggaccggacc aatccccatg cgcacatcat gctgaccacg
      421 agagctatcg gggaaacggg attcgcagga aaggtcaggg actggaacga ccgggcactc
      481 gccgagacgt ggcgcgcatc atgggctgac catgcgaaca gagcgcttgc gaacgccggc
      541 taccaggaag agatagacca tcgttcatac gagcgtcagg gactggagaa agcgccgggc
      601 cttcacctcg gaaaggctgc ctgtgcgatg gaaaaacggg gaatggaaac agaacgcggt
      661 gagcagaacc gtctgattaa cagccttaac ctggaaatac aggtttcccg cacgcagctt
      721 gctctcagga cggttcagga aacgcagcgt aagcgggaac tcagcgatgc tgcacgtcgt
      781 gcagcggaag cccttaacct gaccattccc gctgcgaatg cctcagcgga taccctgcgg
      841 gaattcattg ccacgctgcc gcaggaatgc gggaacgcgt gggagatgac cccggagttc
      901 ctggcgatga gcgggaaggt gaacgacatc gaacgtgagg ggaatgcgct gctgaaagag
      961 caggccattc tcgaaaagga gatgaccgga ctcaaaaaag cacgccctgt cgcgtccctt
     1021 ctgtcagaga ttcccctgat gacatgggct gaaccggaat accgcaaaag acaactccgt
     1081 ttctggaaac tcgggaaaca gattgaatct cttcgccgca cctacagggc cgtgaaagaa
     1141 cgggacattc ccgcccgtcg tcaggccttt gaaacgcagt ggaatacgtg gattgcgccg
     1201 ggaatggcag agctgaaaga aaaactgtca gcacgggaag cggagcggcg cagggaggag
     1261 gccgaagcgg aagcgcgccg gaaggaacag gagcatgagg cgcggctgaa acgtcatgat
     1321 aaccaccgtc tgagccgtga aacggcatta gtcggggtta ttacggatct ggggcgtgcc
     1381 agagagccgg gaacgggcag gataacccgc tacatgatgc tgagtaacag agccggagaa
     1441 ttcacggtat ggggtgatga gctggcgcat tacccccaga gtgttcatga cccggtgaat
     1501 gtttacctgt cgccaggcgg ggctgtgatg gtctcggata tacgtgaggg aatgccagaa
     1561 tctcatgaga cgatggcgcg gcctgagcgt gtgagaatgt attccggtgc gacggtccgg
     1621 catgtactgg aacagatgcg ccaggggtgg ccctcttacg gttttccggc gctgccgcat
     1681 cactggccgg ataattttta tttcagcgac gaccgcaggc ccgtagcctc tccgctgccg
     1741 tcagcgcagc gggtggatat caccgctgat gctgtaccgg aacaactcat gcctgttgta
     1801 ttttcgagag agggaaacag caggacgctg aatctgttgt tatgcaaagg gccggaggaa
     1861 atgcttgtcg ggtttgttcg ccgggaagga gggttttgcc ccgttcttgt gcttccgtcg
     1921 ccggattaca gtcatctgat agtcagcacc atcacggaga acggggtatg cctggcaggt
     1981 tacggagaag ctataaacca tgatgaggat acaccgtacc caccggaacc gcacctgatg
     2041 cagttccggc tcaaaggcca tcatgacagg cttttggctg ctgtccacaa accggaagag
     2101 atgccggatt atctcttccg tcaactcggt tttaatcaga cctggcatga gtggaagcgg
     2161 gacgaacagc acaggcaaca acaacgccgc cccgggcact tccggggcat gagtatgtga
     2221 tgtccggggc tgcaccccgg accccgccaa cacatcacgg gccacaaaat tttttgtggc
     2281 ccgctctgcg ttttctaagt gttatccctc ctgatttcta aaaaattttc cacctgagct
     2341 tgacagaaaa aacgatgacg ggtacttttt gatctgtaca taaaaccagt ggttttatgt
     2401 acagtattaa tcatgtaatt aattgtttta acgcttaaaa gagggaattt ttatgagcgg
     2461 tggtgatgga cgcggccata acacgggcgc gcatagcaca agtggtaaca ttaatggtgg
     2521 cccgaccggg cttggtgtag gtggtggtgc ttctgatggt tccgggtgga gttcggaaaa
     2581 taacccgtgg ggtggtggtt ccggtagcgg cattcactgg ggaggtggtt ccggtcatgg
     2641 taatggcggg gggaatggta attccggtgg tggctcggga acaggcggta atctgtcagc
     2701 agtagctgcg ccagtggcat ttggttttcc ggcactttcc actccaggag ctggcggtct
     2761 ggcggtcagt atttcagcgg gagcattatc ggcagctatt gctgatatta tggctgccct
     2821 gaaaggaccg tttaaatttg gtctttgggg ggtggcttta tatggtgtat tgccatcaca
     2881 aatagcgaaa gatgacccca atatgatgtc aaagattgtg acgtcattac ccgcagatga
     2941 tattactgaa tcacctgtca gttcattacc tctcgataag gcaacagtaa acgtaaatgt
     3001 tcgtgttgtt gatgatgtaa aagacgaacg acagaatatt tcggttgttt caggtgttcc
     3061 gatgagtgtt ccggtggttg atgcaaaacc taccgaacgt ccgggtgttt ttacggcatc
     3121 aattccaggt gcacctgttc tgaatatttc agttaataac agtacgccag cagtacagac
     3181 attaagccca ggtgttacaa ataatactga taaggatgtt cgcccggcag gatttactca
     3241 gggtggtaat accagggatg cagttattcg attcccgaag gacagcggtc ataatgccgt
     3301 atatgtttca gtgagtgatg ttcttagccc tgaccaggta aaacaacgtc aggatgaaga
     3361 aaatcgccgt cagcaggaat gggatgctac gcatccggtt gaagcggctg agcgaaatta
     3421 tgaacgcgcg cgtgcagagc tgaatcaggc aaatgaagat gttgccagaa atcaggagcg
     3481 acaggctaaa gctgttcagg tttataattc gcgtaaaagc gaacttgatg cagcgaataa
     3541 aactcttgct gatgcaatag ctgaaataaa acaatttaat cgatttgccc atgacccaat
     3601 ggctggcggt cacagaatgt ggcaaatggc cgggcttaaa gcccagcggg cgcagacgga
     3661 tgtaaataat aagcaggctg catttgatgc tgctgcaaaa gagaagtcag atgctgatgc
     3721 tgcattgagt tctgctatgg aaagcaggaa gaagaaagaa gataagaaaa ggagtgctga
     3781 aaataattta aacgatgaaa agaataagcc cagaaaaggt tttaaagatt acgggcatga
     3841 ttatcatcca gctccgaaaa ctgagaatat taaagggctt ggtgatctta agcctgggat
     3901 accaaaaaca ccaaagcaga atggtggtgg aaaacgcaag cgctggactg gagataaagg
     3961 gcgtaagatt tatgagtggg attctcagca tggtgagctt gaggggtatc gtgccagtga
     4021 tggtcagcat cttggctcat ttgaccctaa aacaggcaat cagttgaaag gtccagatcc
     4081 gaaacgaaat atcaagaaat atctttgaga ggaagttatg ggacttaaat tggatttaac
     4141 ttggtttgat aaaagtacag aagattttaa gggtgaggag tattcaaaag attttggaga
     4201 tgacggttca gttatggaaa gtctaggtgt gccttttaag gataatgtta ataacggttg
     4261 ctttgatgtt atagctgaat gggtaccttt gctacaacca tactttaatc atcaaattga
     4321 tatttccgat aatgagtatt ttgtttcgtt tgattatcgt gatggtgatt ggtgatcaaa
     4381 tattatcagg gatgagttga tatacgggct tctagtgttc atggatgaac gctggagcct
     4441 ccaaatgtag aaatgttata tttttttatt gggttcttgg ttataattgc tccgcaatga
     4501 tttaaataag cattatttaa atcattctca ggagaggtga aggtggagct aaaaaaagta
     4561 ttggtgatta cactgaaacc gaattcaaaa aaattattga agacatcatc aattgtgaag
     4621 gtgatgaaaa aaaacaggat gataacctcg agtattttat aaatgttact gagcatccta
     4681 gtggttctga tctgatttat tacccagaag gtaataatga tggtagccct gaaggtgtta
     4741 ttaaagagat taaagaatgg cgagccgcta acggtaagtc aggatttaaa cagggctgaa
     4801 atatgaatgc cggttgttta tggatgaatg gctggcattc tttcacaaca aggagtcgtt
     4861 atgaaaaaaa taacagggat tattttattg cttcttgcag tcattattct gtctgcatgt
     4921 caggcaaact atatccggga tgttcagggc gggaccgtat ctccgtcatc aacagctgaa
     4981 gtgaccggat tagcaacgca gtaacccgaa atcctctttg acaaaaacaa agcgtgtcag
     5041 gctgattctg atgcgctttt ttttgaaatg tcacaaaaat tccatgtggg agatgggatc
     5101 taaaatcctc gtgcagaact ttccatccag ggggagaaaa cttgtcgttt tgagccgttc
     5161 ggtgttcaga acgcacgaaa ccgatcgcgc atcgctttcg tgaatagtta tgcaggcccc
     5221 tgaaaacgat tctgacgagt tttttcggtt ttgcctggtg ttttcctgtc tttttgcgtt
     5281 ttttgcgtca gaacgcgtct gagggcgttt taaggggtgc gtgcaacggg agttatggta
     5341 aatggatcgg cttttcggga aggttcgaca ggatttgccg ttgggtgtag tgtaagcgac
     5401 tgaaaaacaa acgccccgta aatcgtgctc tcaccgccaa gattgatcac gaaattacag
     5461 ggcgccgggt tccgcgtttc ccgatgggaa agcgcggtta gttaaactgt gtaccgagag
     5521 aaatcgtatc acatgagcgc cgtacttcaa cgcttcaggg aaaaattacc gcacaaaccg
     5581 tactgtacga acgatttcgc gtacggcgtt cgcattctgc cgaaaaacat tgccattctt
     5641 gcccgtttca tccagcagaa ccagccacat gcactgtact ggcttccctt tgacgtggac
     5701 cggacggggg catcaatcga ctggagcgac cggaattgtc cggccccgaa catcaccgta
     5761 aaaaatcccc gtaacgggca cgcgcatctg ctctacgcgc tcgcccttcc tgtgagaact
     5821 gcgccggatg catcggcttc ggcgctcaga tacgctgccg ctattgagcg tgcgttgtgt
     5881 gaaaaactgg gcgcggatgt gaattacagc ggcctgatct gcaaaaatcc gtgccaccct
     5941 gaatggcagg aagtggaatg gcgcgaggaa ccctacactc tcgacgaact ggctgattat
     6001 ctcgatttga gcgcctcagc gcgccgtagc gtcgataaaa attacgggct ggggcgaaac
     6061 tgctatctgt tcgaaaaggg ccgtaaatgg gcttaccggg ctattcgtca gggctggccg
     6121 gcattctcac aatggcttga tgcggtgatt cagcgcgtcg aaatgtacaa cgcatcgctc
     6181 cccgttccgc tttcacctcc tgaatgtcgg gctattggca agagtattgc gaaatacacg
     6241 cacaggaact tcacgccgga aactttcgca cagtatgtgg ctgatacgca cacgccagaa
     6301 attcaggcta cacgcggtcg caagggcggt tctaagtcta agcgcggcac agtagctaca
     6361 tcagcacgca cgctgaaacc ttgggagaaa ttagggatca gtcgcgcctg gtattaccaa
     6421 ctgaaaaaac gaggtctcgt agagtagacc aaataagcct atatcagata acagcgcctt
     6481 tttggcgtct ttttgagcag cttggtttgt cgctatttcc ctcgttgaat cccgcaatgg
     6541 cgcggctttc cgcatgattg aggtggtagc gctcgccgca gtctcatgac cgagcgtagc
     6601 gagcgaatga gcgaggaagc gcaaaggcgt ccggtggtgc atgtggcact tacgcgccgg
     6661 ggcttagtgg ttctgcggtt tcgccggtgg tctgggtagc ttctccagct cgttaatcag
     6721 cggttgtagt cggttcacat ccgcctgtct tgtgacttcc tttcgcagaa actggagcag
     6781 gaacgcacgc agttgcgctt cttccggcct ccgtaccctt gccagcatgg ctgcccccac
     6841 aatgactttt tgcgccgtgt ccaggcttcg gctcttcgcc ttcaggcgct gtaatctggc
     6901 ctcagcttcg gcaatcttct gttcgagtgt tctgctcatt tcgtgactcc gtgcgcggtg
     6961 aaaaatcgca ttttagcgcg tcactggtag tttaaaaact aaactggcat aatgcacggc
     7021 acatcacgaa atgcgcactt atacaatctc cacttcgttt cgattgtgtg cggtctgcga
     7081 cgctaaaaga aaacggcaaa aaggcattac ggcagaa
//
