LOCUS       NZ_CP031900             7769 bp    DNA     circular CON 19-JUN-2024
DEFINITION  Escherichia coli O113:H21 strain FWSEC0010 plasmid unnamed2,
            complete sequence.
ACCESSION   NZ_CP031900
VERSION     NZ_CP031900.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN08768111
            Assembly: GCF_005014055.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli O113:H21
  ORGANISM  Escherichia coli O113:H21
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7769)
  AUTHORS   Tyson,S., Peterson,C.-L., Olson,A., Tyler,S., Cabral,J., Lynch,T.,
            Knox,N., Van Domselaar,G. and Graham,M.
  TITLE     Food and Water Consortium WGS
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7769)
  AUTHORS   Tyson,S., Peters,G.A., Melnychuk,K., Laminman,V. and Kaplen,B.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-AUG-2018) National Microbiology Lab, Public Health
            Agency of Canada, 1015 Arlington Street, Winnipeg, MB R3E3R2, Canada
REFERENCE   3  (bases 1 to 7769)
  AUTHORS   Cabral,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2017) National Microbiology Laboratory, Public
            Health Agency of Canada, 1015 Arlington St, Winnipeg, Manitoba R3E
            3R2, Canada
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: AUG-2018
            Assembly Method                   :: Unicycler;Canu v. 0.4.4.0;1.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 94.45x;51.16x
            Sequencing Technology :: Illumina MiSeq;Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_005014055.1-RS_2024_06_19
            Annotation Date                   :: 06/19/2024 07:24:10
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,035
            CDSs (total)                      :: 4,909
            Genes (coding)                    :: 4,739
            CDSs (with protein)               :: 4,739
            Genes (RNA)                       :: 126
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 94
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 170
            CDSs (without protein)            :: 170
            Pseudo Genes (ambiguous residues) :: 0 of 170
            Pseudo Genes (frameshifted)       :: 76 of 170
            Pseudo Genes (incomplete)         :: 93 of 170
            Pseudo Genes (internal stop)      :: 41 of 170
            Pseudo Genes (multiple problems)  :: 35 of 170
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP031900.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..7769
                     /organism="Escherichia coli O113:H21"
                     /mol_type="genomic DNA"
                     /strain="FWSEC0010"
                     /serotype="O113:H21"
                     /isolation_source="Clinical: Human (Homo sapiens)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1078021"
                     /plasmid="unnamed2"
                     /geo_loc_name="Canada"
                     /collection_date="1991"
                     /collected_by="Laboratory for Foodborne Zoonoses: Public
                     Health Agency of Canada"
     gene            468..611
                     /locus_tag="C8201_RS26220"
     CDS             468..611
                     /locus_tag="C8201_RS26220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_152896351.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDLTPLAAAIGNWIVDLDTQ
                     NS"
                     /name="replication initiation protein"
     gene            complement(806..937)
                     /locus_tag="C8201_RS25355"
                     /old_locus_tag="C8201_26750"
                     /old_locus_tag="CCU06_RS25370"
     CDS             complement(806..937)
                     /locus_tag="C8201_RS25355"
                     /old_locus_tag="C8201_26750"
                     /old_locus_tag="CCU06_RS25370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_072023513.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_077521118.1"
                     /translation="MKIIIFLITILFLSGCQANYIRDVQGGTVAPSSSSELTGIAVQ"
                     /name="colicin release lysis protein"
     gene            967..1257
                     /gene="cki"
                     /locus_tag="C8201_RS25360"
                     /old_locus_tag="C8201_26755"
                     /old_locus_tag="CCU06_RS25375"
     CDS             967..1257
                     /gene="cki"
                     /locus_tag="C8201_RS25360"
                     /old_locus_tag="C8201_26755"
                     /old_locus_tag="CCU06_RS25375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011264161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin K immunity protein"
                     /protein_id="WP_011264161.1"
                     /translation="MHLKYYLHNLPESLIPWILILIFNDNDNTPLLFIFISSIHVLLYP
                     YSKLTISRYIKENTKLKKEPWYLCKLSALFYLLMAIPVGLPSFIYYTLKRN"
                     /name="cki"
     gene            complement(1254..2726)
                     /locus_tag="C8201_RS25365"
                     /old_locus_tag="C8201_26760"
                     /old_locus_tag="CCU06_RS25380"
     CDS             complement(1254..2726)
                     /locus_tag="C8201_RS25365"
                     /old_locus_tag="C8201_26760"
                     /old_locus_tag="CCU06_RS25380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_159421185.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0140911 - pore-forming activity [Evidence
                     IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA];
                     GO:0050829 - defense response to Gram-negative bacterium
                     [Evidence IEA]; GO:0031640 - killing of cells of another
                     organism [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like pore-forming protein"
                     /protein_id="WP_000362089.1"
                     /translation="MDKVTDNSPDVESTESTEGSFPTVGVDTGDTITATLATGTENVGG
                     GGGAFGGASESSAAIHATAKWSTAQLKKHQAEQAARAAAAEAALAKAKSQRDALTQRLK
                     DIVNDALRANAARSPSVTDLAHANNMAMQAEAERLRLAKAEQKAREEAEAAEKALREAE
                     RQRDEIARQQAETAHLLAMAEAAEAEKNRQDSLDEEHRAVEVAEKKLAEAKAELAKAES
                     DVQSKQAIVSRVAGELENAQKSVDVKVTGFPGWRDVQKKLERQLQDKKNEYSSVTNALN
                     SAVSIRDAKKTDVQNAEIKLKEAKDALEKSQVKDSVDTMVGFYQYITEQYGEKYSRIAQ
                     DLAEKAKGSKFSSVDEALAAFEKYKNVLDKKISKVDRDAIFNALESVNYDELSKNLTKI
                     SKSLKITSRVSFLYDVGSDFKNAIETGNWRPLFVTLEKSAVDVGVAKIVALMFSFIVGV
                     PLGFWGIAIVTGIVSSYIGDDELNKLNELLGI"
                     /name="colicin-like pore-forming protein"
     gene            2971..3261
                     /locus_tag="C8201_RS25370"
                     /old_locus_tag="C8201_26765"
                     /old_locus_tag="CCU06_RS25385"
     CDS             2971..3261
                     /locus_tag="C8201_RS25370"
                     /old_locus_tag="C8201_26765"
                     /old_locus_tag="CCU06_RS25385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001672407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_024200900.1"
                     /translation="MSKSPRHKLNKTDKRLLDTLVAAGYEHDKARDLIQKQVYTLTLAD
                     QRHVVSEISNGMNPTQAYSAVYQARRIRLARKYLNGKKVMEETGENTPPSA"
                     /name="hypothetical protein"
     gene            complement(3253..3531)
                     /locus_tag="C8201_RS25375"
                     /old_locus_tag="C8201_26770"
                     /old_locus_tag="CCU06_RS25390"
     CDS             complement(3253..3531)
                     /locus_tag="C8201_RS25375"
                     /old_locus_tag="C8201_26770"
                     /old_locus_tag="CCU06_RS25390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000710675.1"
                     /translation="MKKCVPVLLFSVLSASVASHADSNVFQQKAEKTKNGIVADVNARQ
                     KMFSNRLKIQLQIDELRAELAGTRDAQKREDLRKKLESLERQKKSLR"
                     /name="hypothetical protein"
     gene            3653..4075
                     /locus_tag="C8201_RS25380"
                     /old_locus_tag="C8201_26775"
                     /old_locus_tag="CCU06_RS25395"
     CDS             3653..4075
                     /locus_tag="C8201_RS25380"
                     /old_locus_tag="C8201_26775"
                     /old_locus_tag="CCU06_RS25395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_032319274.1"
                     /translation="MRSAAARLLLIPLITATIALTGCTPKTSLERHTRHYVYASDDGFD
                     PNFYTQKADTIRMMLPFFQQFRDMGMKDKAAGVSAETAQQRVKEFHSEKFFHSLQSTTT
                     FAGRKYTNSDMPSPKKMKLMADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            complement(4134..4400)
                     /locus_tag="C8201_RS25385"
                     /old_locus_tag="C8201_26780"
                     /old_locus_tag="CCU06_RS25400"
     CDS             complement(4134..4400)
                     /locus_tag="C8201_RS25385"
                     /old_locus_tag="C8201_26780"
                     /old_locus_tag="CCU06_RS25400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262326.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_000262325.1"
                     /translation="MVWTINYSDRALKSLRKMDKQNARRIVDFMDLRIAVAADPRKSGR
                     PLKGELGEFWRYRVGDYRILCEIRDDELIILVATIGHRREVYD"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(4384..4608)
                     /locus_tag="C8201_RS25390"
                     /old_locus_tag="C8201_26785"
                     /old_locus_tag="CCU06_RS25405"
     CDS             complement(4384..4608)
                     /locus_tag="C8201_RS25390"
                     /old_locus_tag="C8201_26785"
                     /old_locus_tag="CCU06_RS25405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="WP_000879770.1"
                     /translation="MLAIRLSDEIESRLDSLAKKTGRTKTFYAREAILAHLEDLEDYYL
                     SAETAARVRRGDEAVHSSEDVRKSLGLDD"
                     /name="DUF6290 family protein"
     gene            4762..4974
                     /locus_tag="C8201_RS25395"
                     /old_locus_tag="C8201_26790"
                     /old_locus_tag="CCU06_RS25410"
     CDS             4762..4974
                     /locus_tag="C8201_RS25395"
                     /old_locus_tag="C8201_26790"
                     /old_locus_tag="CCU06_RS25410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000350688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000350688.1"
                     /translation="MDIEMNNTEEMFSKLGMRLGAEMAIPLKDGDNFAPTGCIVTKLDK
                     ENGLLICRPVQKEINGTWYTVDLSK"
                     /name="hypothetical protein"
     gene            complement(5506..5739)
                     /locus_tag="C8201_RS25405"
                     /old_locus_tag="CCU06_RS25420"
     CDS             complement(5506..5739)
                     /locus_tag="C8201_RS25405"
                     /old_locus_tag="CCU06_RS25420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137429.1"
                     /translation="MTELEMHLLNALEQLQQDYTQRLKDWESAFTDLQHMFELTRQENG
                     QLREQCDDLSQQVQRLAGQVDRLSRLFITNSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(5746..6306)
                     /locus_tag="C8201_RS25410"
                     /old_locus_tag="CCU06_RS25425"
     CDS             complement(5746..6306)
                     /locus_tag="C8201_RS25410"
                     /old_locus_tag="CCU06_RS25425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_001345151.1"
                     /translation="MSNLLQMGTDFEKKLKERAVSTENMLNSEFRKLEASVDRELNLNG
                     QKIRDAISAHTAAVKEQLETLNSAVNAQFSATENELARRQEELLWKLVKGRILYPSLTA
                     LCVTVGIFLASWGLIQWQESRIAANILAIRDQEETLAKLREKTWGVTYHEGRNGKFLVL
                     PSGVKGENNWTVEKKNAVRLVRE"
                     /name="MbeB family mobilization protein"
     gene            complement(<6341..7021)
                     /locus_tag="C8201_RS26225"
                     /pseudo=""
     CDS             complement(<6341..7021)
                     /locus_tag="C8201_RS26225"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(6984..7307)
                     /locus_tag="C8201_RS25420"
                     /old_locus_tag="C8201_26810"
                     /old_locus_tag="CCU06_RS25435"
     CDS             complement(6984..7307)
                     /locus_tag="C8201_RS25420"
                     /old_locus_tag="C8201_26810"
                     /old_locus_tag="CCU06_RS25435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_001444404.1"
                     /translation="MLTIRVTDEEHARLLARCEGKQLASWMRKVCLGAPPSKTSGLPTL
                     APPLLRQFASVGNNLNQIARKINSGQWSGHDRVHVVAALMAIGRELSELRDEVRKQGER
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            7375..7566
                     /locus_tag="C8201_RS25425"
                     /old_locus_tag="C8201_26815"
                     /old_locus_tag="CCU06_RS25440"
     CDS             7375..7566
                     /locus_tag="C8201_RS25425"
                     /old_locus_tag="C8201_26815"
                     /old_locus_tag="CCU06_RS25440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165980.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADDQADICEALHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          complement(7655..7739)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="97.674"
                     /coverage="100.0"
                     /evalue="6.569999999999999e-34"
     Typing          2..540
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(5400..6994)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.436"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(7367..7569)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="89.706"
                     /coverage="100.0"
                     /evalue="3.88e-12"
ORIGIN
        1 atgccgcaca gatgcgtaag gagagaatgc acgtccaggc gcttttccgc ttcctcgctc
       61 actgactcgc tccgctcggt cgttcggctg cggcgagcgg actctccttt ctcaaaggcg
      121 gtaaccccgt tatccacaga atccggggat aacccgggaa agaacatgtg cgcaaaaaaa
      181 cgaagacccc gggaaaggcc gcggcacagg cgctttttcc ataggctccg cccccctgac
      241 gagcatcaca aaaaccgacg ctcaagtcag aggtggcgaa acccgacagg acttaaagat
      301 accaggcgtt tccccccgga agctccctcg tgcgctctcc tgttccgacc ctgccgatta
      361 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
      421 gttggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
      481 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
      541 gacacgactt aacgccactg gcagcagcca ttggtaactg gatagtggat ttagatacgc
      601 agaactcttg aagttgaagc cttatagcgg ctacactgga aggacagcat ttggtatctg
      661 tgctccacta aagccagtta cccggttaag cagtccccaa ctgacttaac cttcgactaa
      721 accgcctccc caggcggttt tttcgtttac aggcagcaga ttacgcgcag aaaaaaagga
      781 tctcaagaag atcctttgat cttttctact gaaccgcgat ccccgtcagt tcagaagacg
      841 aagatggtgc aacggttcct ccttgcacat cacgaatata gtttgcttga catcctgaca
      901 agaataatat tgtaattaga aaaattatta ttttcatatt tctttccaac aaaagtaaaa
      961 atacttatgc atttaaaata ctacctacat aatttacctg aatcacttat accatggatt
     1021 cttattttaa tatttaacga caatgataac actcctttgt tatttatatt tatatcatca
     1081 atacatgtat tgctatatcc atactctaaa ttaaccatat ctagatatat caaagaaaat
     1141 acaaagttaa aaaaagaacc ctggtactta tgcaagttat ctgcattgtt ttatttatta
     1201 atggcaatcc cagtaggatt gccaagtttc atatattaca ctctaaagag aaattaaatc
     1261 cctaacaact cattaagttt gttcaactca tcatccccta tgtaagaaga aacaatacct
     1321 gtgacaattg caatccccca gaaaccaaga ggcacaccaa caataaaact aaacattaat
     1381 gccacaattt ttgccacacc tacatcgacc gcacttttct ccagcgtgac aaacaaaggt
     1441 cgccagttac cagtttctat tgcattttta aaatcagaac caacatcata tagaaaagat
     1501 actcgactag tgattttaag cgactttgat atcttcgtta aattcttaga taactcatca
     1561 taattaacag actctaacgc attaaaaata gcatcccgat caaccttact gattttctta
     1621 tccagtacat ttttatactt ttcaaatgca gcaagagctt catcaacact actaaattta
     1681 ctacccttcg ccttttcagc taaatcctga gctattctgg aatatttttc cccatattgt
     1741 tcggttatat attgataaaa cccaaccata gtatcaacag agtcttttac ctgactcttc
     1801 tcaagagcat ccttagcttc ttttaatttt atctcagcat tctgaacatc tgttttttta
     1861 gcatctctaa tgctaaccgc agaattaaga gcattcgtca ctgacgaata ttcattcttc
     1921 ttatcctgca attgtctctc cagttttttc tgaacatcac gccatccagg aaatccagta
     1981 accttcacat caacactttt ttgagcgttt tcaagctccc ctgcaactct ggaaacaatc
     2041 gcttgcttac tctgtacatc gctttcggcc ttcgccagtt cagctttagc ctcagccagc
     2101 ttcttctctg ccacttccac agcccgatgc tcttcatcaa gagaatcctg tcgatttttc
     2161 tcagcctctg ctgcctccgc cattgctaac aaatgcgcgg tttcagcctg ttggcgggca
     2221 atctcatcac gttggcgttc tgcttcccgg agcgcttttt ctgctgcttc agcttcttca
     2281 cgggcttttt gctctgcctt cgcaaggcgc aaacgctcag cctctgcctg cattgccata
     2341 ttattggcat gagcaaggtc agttactgat ggactacggg cggcattagc acgtaaagcg
     2401 tcattaacaa tatccttgag acgttgagtc agggcatcac gctgagattt cgcttttgcc
     2461 aatgctgcct cagccgcagc agcacgggca gcctgttcag cctgatgttt tttcaactgc
     2521 gcggtagacc atttagcggt tgcatgtatc gcagcagaac tttcactggc cccaccaaat
     2581 gctccaccgc ctccaccaac attttcagtt ccagttgcaa gcgtcgctgt aatcgtatcg
     2641 ccagtatcaa ccccaacagt tgggaatgac ccctcagtag attctgtgct ctccacatct
     2701 ggagaattat cagtgacttt atccataaag aaattcctct ttgataatta aaacaataaa
     2761 ttaaaaacaa tatactgtac atataaccac tggttttatg tacagtgaaa acctactact
     2821 cagcattgtc catgtcaaga gcatggattt tcatttttgc aataaggatc acactatggg
     2881 gaggcaggca ttgagaacgt cgaaacagaa caccggagca aatcaggatg agatataaaa
     2941 ctgttggatc atgaaaaaac ggagaacgat gtgagcaaat caccccgcca taaactgaac
     3001 aaaacagaca aacgacttct cgacaccctt gttgctgccg gatatgagca tgacaaagcc
     3061 cgtgacctca tccagaaaca ggtttacacg ctgacactgg cagatcagcg tcatgtggtc
     3121 agtgaaatca gtaatggtat gaatcccacc caggcttact cggcggtata ccaggcaaga
     3181 cgcattcgcc tcgcccgtaa atatctgaac ggtaaaaagg ttatggaaga aaccggggaa
     3241 aatacgcccc catcagcgta aggatttctt ttgccgctcc agagactcca gttttttacg
     3301 caaatcctct cttttttggg catctctggt gccagccagc tctgctctca actcatcgat
     3361 ctgaagttgt atcttcagtc tgttactgaa cattttctgt ctggcattaa catccgcaac
     3421 aatgccgttt tttgtcttct cggccttttg ttgaaaaaca ttgctgtccg catgactggc
     3481 aaccgaagca gaaagaacac taaaaagcag gactggcaca cattttttca cgggattatt
     3541 cctgactcat tgaccatcaa atcacattgg gagtaaaccg acgtatgata agagatactc
     3601 ttcggagata taactccctg agtatcaaga ttaaaaacgc aaggagatgt ttatgagatc
     3661 tgccgctgcc aggctgcttc tgatacctct gataacagca acaatagctc ttacaggatg
     3721 cacaccaaag accagcctgg aacgacatac ccggcattat gtttatgctt cagatgatgg
     3781 atttgatcct aacttctaca cccagaaagc agacaccata cgtatgatgc tcccgttctt
     3841 tcagcagttc cgggatatgg ggatgaaaga caaagcagcc ggagtatcag cagaaacggc
     3901 acagcaacgt gtaaaagaat tccactcaga aaaatttttt cactcactcc agagcacaac
     3961 aacctttgct ggcagaaaat acacaaacag cgatatgcct tcgccgaaaa aaatgaaact
     4021 aatggcagac accatttctg cggtttatct cgatggatac gagggcagac agtaagggat
     4081 ttatcataat cccttattgt acgccacata tgaaaaagcc cgcatggcgg gcttcagtcg
     4141 tagacttcac gtcgatggcc gatggtggcc acaagaataa tcagttcgtc atccctgatt
     4201 tcacacagaa tgcgataatc acccacacga taacgccaga attctcccaa ctcccccttc
     4261 agtggcctcc ctgatttacg gggatcggct gcaactgcaa tacgtaaatc cataaaatcc
     4321 acaatccgtc gcgcgttctg cttgtccatc ttacgtagcg atttgagcgc ccgatcggaa
     4381 taattaatcg tccagaccaa gagacttcct tacgtcctca gacgaatgca cagcctcatc
     4441 accacggcga acacgtgctg ctgtttccgc tgaaagataa taatcttcca gatcttccag
     4501 gtgcgccagt atcgcttccc gggcatagaa cgtctttgtc ctcccggttt tcttcgccag
     4561 cgagtccaga cgggactcaa tttcatcaga caaccgaata gccagcatac aaatctccct
     4621 ccgaacaatt gttatacatg tatagcaaaa attataacgt aaaaagacga taaaacaatc
     4681 aaaggctggt tgaataaaca gtaaaatccc atatattaac gactgatata tgataagtat
     4741 cggttttaaa aggaaaacaa catggatatt gaaatgaata acactgaaga gatgttcagc
     4801 aaactgggga tgcggttagg agctgagatg gcaatccctt tgaaggatgg tgataatttt
     4861 gcgccaactg gatgcatcgt tacgaagctt gataaagaga atggccttct gatctgccga
     4921 ccagttcaga aggaaatcaa cggcacgtgg tacaccgtcg atttaagtaa gtaacaccac
     4981 ggactaaagc cctgcggggc ttttttatac ctgccgctga ctctgattga acataacatc
     5041 tgaattgtac gcaccgctga aatgcgttca gcgcgatcac ggctgctgac aggtaaaaat
     5101 ggcaacaaac cacccgaaaa gctgccgcga tcgcacctga taaattttaa ccgtatgcat
     5161 agctattcag ccatgtgaat aacgctggtt ttgcctgcgt aaacctcatg acactgtttt
     5221 ttttccatct tttcagttga tgacatacgc agacatcgcg ggatgaggct gaggaatgag
     5281 cgcgatctgg caaagaggca aaacacagca acaaaaacga cacgccagaa tcgcgcccgg
     5341 atgcgttttt aacgcgttcc ggtaccatct ggcaacctcc cggaacaact caccgtcaca
     5401 tccccattga cgggccacgc catacccgtg cttcccgttc ctgctcttca tgccaggacc
     5461 gcgcacgctc ccgttccagg cgtgcctgcc tttcctgttc atcccttatc tgctgttcgt
     5521 gataaataac cgactcaagc ggtccacctg cccggctaat ctctgcacct gctgactcaa
     5581 gtcgtcgcac tgttccctca gttgcccgtt ctcctgtcgt gtcagctcga acatatgctg
     5641 caaatccgtg aaggcgctct cccagtcttt cagccgctgc gtatagtcct gctgcaattg
     5701 ctctaaggcg ttcagtaagt gcatttccag ctctgtcata ctcacttact ccctgaccag
     5761 tcttactgcg ttcttcttct ccaccgtcca gttgttttcc cccttcaccc cggacggcaa
     5821 caccagaaat ttcccgttcc tgccctcgtg atacgtcaca ccccatgttt tttcccggag
     5881 tttcgccagc gtctcttcct ggtccctgat agccaggatg ttcgccgcaa tacggctttc
     5941 ctgccactga atcagccccc atgacgccag aaaaatcccg accgtgacgc aaagcgccgt
     6001 cagcgacggg tacagtatcc gccctttgac cagcttccag agcagctctt cctgccgccg
     6061 ggcaagttcg ttctctgtgg cgctgaactg cgcgttcacg gcactgttca gcgtctccag
     6121 ttgttctttc accgctgctg tgtgtgcgct gatagcgtct ctgattttct gcccgtttaa
     6181 gttcagttcc ctgtctacag acgcttcgag cttcctgaac tcgctgttca gcatgttctc
     6241 tgtagagacg gcacgctctt tcagtttctt ctcgaagtct gtccccattt gtaaaagatt
     6301 gctcatacag cgcccctttc agcctgagat tacgcccgcc ctccgggtcg gcgatactga
     6361 tactgctcct ggttgtcctc accacctcaa aacctgccgc tgtaagcgcc tcagtgacat
     6421 cctgacgcgt ttttagcgct ccggcatggt aaagagcctc cagtcccctc gtaatcgctt
     6481 ctgcggcctc ctgtttcgct ttcggcagat tattcggggt gacaagtgtc cgcctgttct
     6541 ccggtgcgtt cgggtcgtgc agcccgtaat ggtgattcac cagtgtctgc caggcattga
     6601 ttcgcggacg gtccgcccgg tcgtaatagg gctggagccg ttttccgctc gccagctcca
     6661 tattcgggat gacaaaattc agctcaagac gccccttgtc ctggtgctcc acccacagga
     6721 tgctgtactg atttttttca agaccgggca tcagtacccg ctcaaagctc tccatcaccc
     6781 tttcacgctc tcccggtggc agtgtctgct ctgcaaaaga cagaaccccc gaggtgtatt
     6841 ttttcgcaaa cggcgtggca tcgatgagtt cccgcacctc ttcgggagca ccccgcagaa
     6901 ctctcgcccc ttcccggtta cgctcccggc ccagcaggta atcaaccgga ccactgccac
     6961 cgccttttcc cctggcatga aacttaacta tcatcgcgtt ctccctgttt acggacctca
     7021 tccctcagct cactcagttc acgtccgatg gccatcagtg cagccaccac atgaacccgg
     7081 tcatgccccg accactgtcc actgtttatc ttccgggcta tctgattcag gttattgccg
     7141 accgaagcga actggcgcaa cagcggcggt gccagtgtcg gaagacctga cgttttcgat
     7201 ggcggtgccc ccaggcagac cttacgcatc catgacgcaa gttgttttcc ctcacaacgt
     7261 gccagcagcc gcgcatgttc ctcatccgtg acccgtatcg tgagcatcct ttcgcgtttc
     7321 accggtatca ttaaaaacct ccgacagact ccccacacat ggagaaacag aactgtgact
     7381 aaacaggaaa aaaccgccct taacatggcc cgcttcatca gaagccagac gctgaccctg
     7441 ctggaaaaac tgaatgaact ggacgccgac gaccaggctg acatctgcga agcgcttcac
     7501 gaccacgctg acgagcttta ccgcagctgc ctcgcacgct tcggggatga cggtgaaaac
     7561 ctctgacaca tgcagctccc ggagacggtc acagcttgcc tgtgagcgga tgccgggagc
     7621 agacaagccc gtcagggcgc gtcagcgggt cttagcgggt gtcggggcgc agccatgacc
     7681 cagtcacgta gcgatagcgg agtgtatact ggcttaatta tgcggcaaca gtgcggattg
     7741 tatgaaaagt gcgccatgcc gggtgtgaa
//
