LOCUS       NZ_CP031891            27068 bp    DNA     circular CON 17-OCT-2023
DEFINITION  Staphylococcus aureus strain CFSAN082781 plasmid pMRSA_22, complete
            sequence.
ACCESSION   NZ_CP031891
VERSION     NZ_CP031891.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN09882579
            Assembly: GCF_008329885.1
KEYWORDS    RefSeq.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 27068)
  AUTHORS   Hoffmann,M., Sanchez,M., Worley,J. and Bry,L.
  TITLE     Whole genome sequencing of cultured pathogen
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 27068)
  AUTHORS   Hoffmann,M., Sanchez,M., Worley,J. and Bry,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-AUG-2018) CFSAN-ORS-DM-MMSB, US Food and Drug
            Administration, 5100 Paint Branch Parkway, College Park, MD 20740,
            USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 210x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 10/17/2023 01:51:41
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,920
            CDSs (total)                      :: 2,841
            Genes (coding)                    :: 2,754
            CDSs (with protein)               :: 2,754
            Genes (RNA)                       :: 79
            rRNAs                             :: 6, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 5, 5 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 45 of 87
            Pseudo Genes (incomplete)         :: 43 of 87
            Pseudo Genes (internal stop)      :: 18 of 87
            Pseudo Genes (multiple problems)  :: 18 of 87
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP031891.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            This genome has a base modification file available.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..27068
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /strain="CFSAN082781"
                     /isolation_source="nasal perirectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /plasmid="pMRSA_22"
                     /geo_loc_name="USA:MA"
                     /collection_date="2018-04"
                     /collected_by="Clinical Micorbiology Laboratory @ Brigham
                     and Women's Hospital"
     gene            join(26716..27068,1..529)
                     /locus_tag="D1E87_RS14765"
                     /old_locus_tag="D1E87_15090"
                     /pseudo=""
     CDS             join(26716..27068,1..529)
                     /locus_tag="D1E87_RS14765"
                     /old_locus_tag="D1E87_15090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015581923.1"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA];
                     GO:0000155 - phosphorelay sensor kinase activity [Evidence
                     IEA]; GO:0004673 - protein histidine kinase activity
                     [Evidence IEA]"
                     /GO_process="GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /name="sensor histidine kinase"
     gene            645..1574
                     /locus_tag="D1E87_RS14770"
                     /old_locus_tag="D1E87_15095"
     CDS             645..1574
                     /locus_tag="D1E87_RS14770"
                     /old_locus_tag="D1E87_15095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000074567.1"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]; GO:0005524 - ATP binding [Evidence IEA];
                     GO:0016887 - ATP hydrolysis activity [Evidence IEA];
                     GO:0042626 - ATPase-coupled transmembrane transporter
                     activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_000793446.1"
                     /translation="MKNLNNIIEINNLTKQYGSQIAVNNLSITVESGQIYGFLGRNGAG
                     KTTTIRMLLGLIKPTKGNIKIFEKEFESNKTEILKNIGAMVETPGFYSNLTAKENLTIN
                     AKILGIQKENAIEEVLEIVGLASEKVKLFKNYSLGMKQRLGIARALLHNPELLILDEPT
                     NGLDPIGIKEIRKLIKDLAQSRNITILISSHILSEIEQLVDKVGIIHNGVLLEEIMYED
                     LKKKNRQFISISVSDEEKVACLLETNFGIYDYKIKENGEFKIYSHIDRQQEINRLLVLN
                     NIDVFQMRVIEDNLETYFEDLVGGGTIG"
                     /name="ABC transporter ATP-binding protein"
     gene            1567..2304
                     /locus_tag="D1E87_RS14775"
                     /old_locus_tag="D1E87_15100"
     CDS             1567..2304
                     /locus_tag="D1E87_RS14775"
                     /old_locus_tag="D1E87_15100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000369394.1"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]; GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="WP_000369394.1"
                     /translation="MDNLLRTEFIKLKRSYMFLISILGTCVAPFMVVVASFISMRTKVP
                     TPNIVFKQLFEEVSLYHMLIIGPPLYGVIIAYLFGREYIEDTLKNLLIIPISRESFLVS
                     KIILLFLWIQTLSVIAWLLTLMLGMLLHFQGLSQSLIIDSFFTFVIGGVLLWILSPPII
                     ILSIYFKNYVPTIVLTIIITLINLMTANSEHRGLFPWAAVGDISRGTLPNTYPFLLSLI
                     IIIVTSIFSVIILFSYFKKTDIS"
                     /name="ABC transporter permease"
     gene            2318..>2494
                     /locus_tag="D1E87_RS14780"
                     /old_locus_tag="D1E87_15105"
                     /pseudo=""
     CDS             2318..>2494
                     /locus_tag="D1E87_RS14780"
                     /old_locus_tag="D1E87_15105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499250.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA]"
                     /GO_process="GO:0016311 - dephosphorylation [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /name="undecaprenyl-diphosphate phosphatase"
     gene            2551..3225
                     /locus_tag="D1E87_RS14785"
                     /old_locus_tag="D1E87_15110"
     CDS             2551..3225
                     /locus_tag="D1E87_RS14785"
                     /old_locus_tag="D1E87_15110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105942.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS257 family transposase"
                     /protein_id="WP_001105956.1"
                     /translation="MNYFRYKQFNKDVITVAVGYYLRYALSYRDISEILRERGVNVHHS
                     TIYRWVQEYAPILYQIWKKKHKKAYYKWRIDETYIKIKGKWSYLYRAIDTEGHTLDIWL
                     RKQRDNHSAYAFIKRLIKQFGKPQKVITDQAPSTKVAMVKVIKAFKLKPDCHCTSKYLN
                     NLIEQDHRHIKVRKTRYQSINTAKNTLKGIECIYALYKKNRRSLQIYGFSPCHEISIML
                     AS"
                     /name="IS6-like element IS257 family transposase"
     gene            <3366..4001
                     /locus_tag="D1E87_RS14790"
                     /old_locus_tag="D1E87_15115"
                     /pseudo=""
     CDS             <3366..4001
                     /locus_tag="D1E87_RS14790"
                     /old_locus_tag="D1E87_15115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009251744.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
                     /name="aminoglycoside 6-adenylyltransferase"
     gene            3998..4528
                     /gene="sat4"
                     /locus_tag="D1E87_RS14795"
                     /old_locus_tag="D1E87_15120"
     ARG             3999..4525
                     /name="sat4"
                     /gene="sat4"
                     /gene_name="streptothricin N-acetyltransferase Sat4"
                     /tool="amrfinderplus"
                     /drug_class="STREPTOTHRICIN"
                     /identity="97.78"
                     /coverage="100.0"
     gene            4621..5415
                     /gene="aph(3')-IIIa"
                     /locus_tag="D1E87_RS14800"
                     /old_locus_tag="D1E87_15125"
     ARG             4622..5412
                     /name="aph(3')-IIIa"
                     /gene="aph(3')-IIIa"
                     /gene_name="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            5691..6262
                     /locus_tag="D1E87_RS14810"
                     /old_locus_tag="D1E87_15135"
                     /pseudo=""
     CDS             5691..6262
                     /locus_tag="D1E87_RS14810"
                     /old_locus_tag="D1E87_15135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079940.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /name="HTH domain-containing protein"
     gene            complement(6431..7105)
                     /locus_tag="D1E87_RS14820"
                     /old_locus_tag="D1E87_15145"
     CDS             complement(6431..7105)
                     /locus_tag="D1E87_RS14820"
                     /old_locus_tag="D1E87_15145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105942.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS257 family transposase"
                     /protein_id="WP_001105991.1"
                     /translation="MNYFRYKQFNKDVITVAVGYYLRYALSYRDISEILRERGVNVHHS
                     TVYRWVQEYAPILYQIWKKKHKKAYYKWRIDETYIKIKGKWSYLYRAIDAEGHTLDIWL
                     RKQRDNHSAYAFIKRLIKQFGKPQKVITDQAPSTKVAMVKVIKAFKLKPDCHCTSKYLN
                     NLIEQDHRHIKVRKTRYQSINTAKNTLKGIECIYALYKKNRRSLQIYGFSPCHEISIML
                     AS"
                     /name="IS6-like element IS257 family transposase"
     gene            7367..7552
                     /locus_tag="D1E87_RS15025"
     CDS             7367..7552
                     /locus_tag="D1E87_RS15025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001037130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001037130.1"
                     /translation="MNKLEKWSLVFIIFTFLSSLALLYFKYNFSSVMPVLSLSILTILI
                     LLTSIITVVSVNDEDT"
                     /name="hypothetical protein"
     gene            complement(7775..8353)
                     /locus_tag="D1E87_RS14825"
                     /old_locus_tag="D1E87_15150"
     CDS             complement(7775..8353)
                     /locus_tag="D1E87_RS14825"
                     /old_locus_tag="D1E87_15150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690624.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_000690637.1"
                     /translation="MKIGYARVSTGLQNLNLQEDRLNTYGCEKIFNDHMSGSKSKRPGL
                     DKAIEFARSGDTIVVWRLDRLGRNMEDLITLVNELNERGVSFHSLEENITMDKSSSTGQ
                     LLFHLFAAFAEFERNLILERSSAGRIAARARGRYGGRPEKLNKQDLNLLKTLYDNGTPI
                     KTIAEQWQVSRTTIYRYLNKLEAKENKNK"
                     /name="recombinase family protein"
     gene            complement(8617..8997)
                     /gene="blaI"
                     /locus_tag="D1E87_RS14830"
                     /old_locus_tag="D1E87_15155"
     ARG             complement(8621..8997)
                     /name="blaI"
                     /gene="blaI"
                     /gene_name="penicillinase repressor BlaI"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="98.41"
                     /coverage="100.0"
     gene            complement(8987..10744)
                     /gene="blaR1"
                     /locus_tag="D1E87_RS14835"
                     /old_locus_tag="D1E87_15160"
     ARG             complement(8991..10744)
                     /name="blaR1"
                     /gene="blaR1"
                     /gene_name="beta-lactam sensor/signal transducer BlaR1"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="95.38"
                     /coverage="100.0"
     gene            10851..11696
                     /gene="blaZ"
                     /locus_tag="D1E87_RS14840"
                     /old_locus_tag="D1E87_15165"
     ARG             10852..11693
                     /name="blaZ"
                     /gene="blaZ"
                     /gene_name="penicillin-hydrolyzing class A beta-lactamase
                     BlaZ"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     gene            complement(12222..12641)
                     /locus_tag="D1E87_RS14850"
                     /old_locus_tag="D1E87_15175"
     CDS             complement(12222..12641)
                     /locus_tag="D1E87_RS14850"
                     /old_locus_tag="D1E87_15175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418034.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003700 - DNA-binding transcription factor activity
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="WP_000418035.1"
                     /translation="MELDNCINYLLSTSQNRVFKHFKSLLSPYNITPAEYGVLNCLINS
                     NLNTPKAIGNTLNLEPSTISGILDKMSKKDLITRQTDNENRRTVLINSTNKAKDLWPTL
                     EKKAYQLNNHYFANLTNEETVVFKKILLKINKTTF"
                     /name="MarR family transcriptional regulator"
     gene            12765..13736
                     /locus_tag="D1E87_RS14855"
                     /old_locus_tag="D1E87_15180"
     CDS             12765..13736
                     /locus_tag="D1E87_RS14855"
                     /old_locus_tag="D1E87_15180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000481285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="WP_000481279.1"
                     /translation="MFNAVVVRKSASNVSYKVEKVDESHLSKGDVLVKVVYSSINYKDM
                     LALQYKGGVIRDYPMIPGIDFAGIVESSSNDKFKEGDNVLVTGYDVGVTHTGGFSEYAQ
                     VPGEWIVPIPQEMSIKESMVYGTAGFTAALSIFELEKYGMNIKSQPEILVSGATGGVGS
                     VAIQILHKIGYENITAIVRKEKQIEIAKKLGAKQVIIIEDDNKPLKKGIFDYFLDTVGS
                     NVTLYGLKRLNYQGVATVCGNVAGNSLNANILPFILRGIKLIGIDSVYVDHNIREDIWS
                     KLANEWNIGNSLLYNEIGFEEFYKTIDQLLKGQHLGRTILKV"
                     /name="oxidoreductase"
     gene            complement(13908..14000)
                     /locus_tag="D1E87_RS14860"
                     /old_locus_tag="D1E87_15185"
     CDS             complement(13908..14000)
                     /locus_tag="D1E87_RS14860"
                     /old_locus_tag="D1E87_15185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002495832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="WP_001791613.1"
                     /translation="MLIIFVHIIAPVISGCAVAYYTYWLSKRNK"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            complement(14157..14504)
                     /locus_tag="D1E87_RS14865"
                     /old_locus_tag="D1E87_15190"
     CDS             complement(14157..14504)
                     /locus_tag="D1E87_RS14865"
                     /old_locus_tag="D1E87_15190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020366422.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003700 - DNA-binding transcription factor activity
                     [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="WP_000119690.1"
                     /translation="MSYENTCDVICVHEDKVNNALSFLEDDKSKKLLNILEKICDEKKL
                     KIILSLIKEDELCVCDISLILKMSVASTSHHLRLLYKNEVLDFYKDGKMAYYFIKDDEI
                     REFFSKNHEGF"
                     /name="metalloregulator ArsR/SmtB family transcription
                     factor"
     gene            complement(14523..15140)
                     /gene="cadD"
                     /locus_tag="D1E87_RS14870"
                     /old_locus_tag="D1E87_15195"
     ARG             complement(14527..15140)
                     /name="cadD"
                     /gene="cadD"
                     /gene_name="cadmium resistance transporter CadD"
                     /tool="amrfinderplus"
                     /drug_class="CADMIUM"
                     /identity="100.0"
                     /coverage="100.0"
     gene            complement(16256..16450)
                     /locus_tag="D1E87_RS14875"
                     /old_locus_tag="D1E87_15200"
     CDS             complement(16256..16450)
                     /locus_tag="D1E87_RS14875"
                     /old_locus_tag="D1E87_15200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001004925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001004925.1"
                     /translation="MNENLEKYIKSLPLLGLLISILLIVLFFFILDVDEDLFVVFLYCL
                     LPLIVNLSIYGFYVFVKRK"
                     /name="hypothetical protein"
     gene            16560..17252
                     /locus_tag="D1E87_RS14880"
                     /old_locus_tag="D1E87_15205"
     CDS             16560..17252
                     /locus_tag="D1E87_RS14880"
                     /old_locus_tag="D1E87_15205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative immunity/bacteriocin fusion bifunctional
                     protein"
                     /protein_id="WP_000691527.1"
                     /translation="MKIIKLFSIISLFLLVFSANFSNAYANEEQKSSLLENQKEKLKSK
                     NIEVSQMTKKEISFLEQLQNKNEDMQKFEKQMKKEGFKKIKLEDKNSKLKYLATEANDK
                     NAYGYIANNAYKNEKTKEIVVSETIYDNYNKKITKFIAEKRSMTENKEGKILINKNYVE
                     YKAEKVDKNRPVTYAGFKWNGKAFACSMAGLYACAHYCGVWAIVNPIAGGVCEAVCGTA
                     FAAGCSVG"
                     /name="putative immunity/bacteriocin fusion bifunctional
                     protein"
     gene            complement(17420..17569)
                     /locus_tag="D1E87_RS15030"
     CDS             complement(17420..17569)
                     /locus_tag="D1E87_RS15030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000695366.1"
                     /translation="MKIKTFATFTHRQLDKQVNRFLKNDIHVLEIQTHSNWLYISAIVF
                     YEDK"
                     /name="hypothetical protein"
     gene            complement(18116..>18405)
                     /locus_tag="D1E87_RS14885"
                     /old_locus_tag="D1E87_15210"
                     /pseudo=""
     CDS             complement(18116..>18405)
                     /locus_tag="D1E87_RS14885"
                     /old_locus_tag="D1E87_15210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009384749.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA]"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /name="protein rep"
     gene            complement(18425..19408)
                     /locus_tag="D1E87_RS14890"
                     /old_locus_tag="D1E87_15215"
     CDS             complement(18425..19408)
                     /locus_tag="D1E87_RS14890"
                     /old_locus_tag="D1E87_15215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="WP_001181105.1"
                     /translation="MQNQYFTVQENYKERFYQIPKVFFTSENYKNLTNDMKIAYAILRD
                     RLNLSIKNSWVDEDGNIYFVYSNEKLMEILNCKKEKLTKIKKGLENDGLLIQKRRGLNK
                     PNILYLMKPIVTERDIYKIEKEENDVEPYGEKEVRKSNVQKFDFRTSRSSKIERPEVRK
                     SNTNDTDFNDIDFNDTENNDMNDLNDIKYKNEISNHSNHSNQFTHNFDDKEMLLQEFPE
                     QLTNYLLKYDYRDLEIIKAVILKAKKSFNSRHEDMHYMLEDIEDEILTSLKRLKKAIHD
                     RGVKGQKETIKSMQAYLMQTILTELEETHALYMRRKNMKQYNIFNQ"
                     /name="replication initiator protein A"
     gene            19834..20577
                     /locus_tag="D1E87_RS14895"
                     /old_locus_tag="D1E87_15220"
     CDS             19834..20577
                     /locus_tag="D1E87_RS14895"
                     /old_locus_tag="D1E87_15220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_000843732.1"
                     /translation="MKSVKKLSEELGVSKQTIFNNIKRLNIETIKQENTSFIKEDTDIE
                     KIIQRVNENKKKYGFESTTEDKQKKGSDNINFESKSDAQIVEILKNQIDTLNNQIEKQE
                     SRHETTIEFYRKELQERSKLLENQQVLALESNKKIQKLENQLEKEKHLNYPFDTSTNTR
                     QNVDAQEKTYTTNPVNINRDQEETKETEIQYKDISGSQSDESTQGEEAKKEDVPANLND
                     NDSDIEEKSEETEPKKGFWSRLFGS"
                     /name="DUF536 domain-containing protein"
     gene            complement(20804..20989)
                     /locus_tag="D1E87_RS14900"
                     /old_locus_tag="D1E87_15225"
     CDS             complement(20804..20989)
                     /locus_tag="D1E87_RS14900"
                     /old_locus_tag="D1E87_15225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011276570.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_000797248.1"
                     /translation="MKNRIREYRKQKGISQETLSKELKVSRQTINAIENNKYDPTLTLA
                     FKLARLFDTTVDELFS"
                     /name="helix-turn-helix transcriptional regulator"
     gene            complement(20994..21332)
                     /locus_tag="D1E87_RS14905"
                     /old_locus_tag="D1E87_15230"
     CDS             complement(20994..21332)
                     /locus_tag="D1E87_RS14905"
                     /old_locus_tag="D1E87_15230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001049146.1"
                     /translation="MNLIPFIIILIFSCLWTAITNKYLPSETLDKKGQQDRYDERQRKM
                     FIEILAKSFIWIVYCMLFTLVLKFTGLSPSIEGSWFSQYPEIFFIIVALFLMLFNYYTT
                     NKKYTSKG"
                     /name="hypothetical protein"
     gene            complement(21578..22186)
                     /locus_tag="D1E87_RS14910"
                     /old_locus_tag="D1E87_15235"
     CDS             complement(21578..22186)
                     /locus_tag="D1E87_RS14910"
                     /old_locus_tag="D1E87_15235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002474535.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_000583528.1"
                     /translation="MIIGYARVSSIDQNLERQLDNLKTFGVEKIFTEKQSGKSVENRPV
                     FQEALNFVRMGDRFVVESIDRLGRNYDEIIETVNYLKEKDVQLMITSLPMMNEVIGNPL
                     LDKFMKDLIIQILAMVSEQERNESKRRQAQGIQVAKEKGVYKGRPLLYSPNAKDPQKRV
                     IYHRVVEMLEEGKAISKIAKEVNITRQTVYRIKHDKGLS"
                     /name="recombinase family protein"
     gene            complement(22672..23571)
                     /locus_tag="D1E87_RS14915"
                     /old_locus_tag="D1E87_15240"
     CDS             complement(22672..23571)
                     /locus_tag="D1E87_RS14915"
                     /old_locus_tag="D1E87_15240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mph(C) family macrolide 2'-phosphotransferase"
                     /protein_id="WP_000196697.1"
                     /translation="MTRHNEIIKCAEKYQLHIQPQTISLNESGLDFQVAFGKDKHGVEW
                     VLRLPRRPDVYKRTKPEKQTVDFLQKNVSFEIPKWKVHAKDLIAYPKLTGKPAATIDPE
                     IQNYVWEIEHKPLPENFINTLAETLVDLHNIPEENINVQHINIKTIQEIKNDFQRRMNK
                     VKETYGVSDELWNRWKQWLENDELWPRHATMIHGDLHPGHIMVDNQANVTGLIDWTEAT
                     HSDPSMDFIGHHRVFDDEGLEQLITAYGKAGGEIWPRMKEHIIELNAVFPMFIAEFAME
                     SGESAYETMALKELGMKE"
                     /name="Mph(C) family macrolide 2'-phosphotransferase"
     gene            complement(23670..25136)
                     /gene="msr(A)"
                     /locus_tag="D1E87_RS14920"
                     /old_locus_tag="D1E87_15245"
     ARG             complement(23674..25136)
                     /name="msr(A)"
                     /gene="msr(A)"
                     /gene_name="ABC-F type ribosomal protection protein Msr(A)"
                     /tool="amrfinderplus"
                     /drug_class="MACROLIDE/STREPTOGRAMIN"
                     /identity="100.0"
                     /coverage="100.0"
     gene            25610..26284
                     /locus_tag="D1E87_RS14925"
                     /old_locus_tag="D1E87_15250"
     CDS             25610..26284
                     /locus_tag="D1E87_RS14925"
                     /old_locus_tag="D1E87_15250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105942.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="WP_001105960.1"
                     /translation="MNYFRYKQFNKDVITVAVGYYLRYALSYRDISEILRERGVNVHHS
                     TIYRWVQEYAPILYQIWKKKHKKAYYKWRIDETYIKIKGKWSYLYRAIDTEGHTLDIWL
                     RKQRDNHSAYAFIKRLIKQFGKPQKVVTDQAPSTKAAMAKIIKAFKLKPDCHCTSKYLN
                     NLIEQDHRHIKVRKTRYQSINTAKNTLKGIECIYALYKKNRRSLQIYGFSPCHEISIML
                     AS"
                     /name="IS6 family transposase"
     gene            <26342..26719
                     /locus_tag="D1E87_RS14930"
                     /old_locus_tag="D1E87_15255"
                     /pseudo=""
     CDS             <26342..26719
                     /locus_tag="D1E87_RS14930"
                     /old_locus_tag="D1E87_15255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019834602.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]; GO:0006355 - regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /name="response regulator transcription factor"
     ARG             complement(22676..23571)
                     /name="mph(C)"
                     /gene="mph(C)"
                     /gene_name="Mph(C) family macrolide 2'-phosphotransferase"
                     /tool="amrfinderplus"
                     /drug_class="MACROLIDE"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             complement(23671..25136)
                     /name="msrA"
                     /gene="msrA"
                     /gene_name="msr-type ABC-F protein"
                     /tool="rgi"
                     /drug_class="macrolide antibiotic; streptogramin
                     antibiotic; streptogramin B antibiotic"
                     /identity="98.36"
                     /coverage="100.0"
     ARG             complement(22673..23571)
                     /name="mphC"
                     /gene="mphC"
                     /gene_name="macrolide phosphotransferase (MPH)"
                     /tool="rgi"
                     /drug_class="macrolide antibiotic"
                     /identity="98.66"
                     /coverage="100.0"
     ARG             4011..4528
                     /name="SAT-4"
                     /gene="SAT-4"
                     /gene_name="streptothricin acetyltransferase (SAT)"
                     /tool="rgi"
                     /drug_class="nucleoside antibiotic"
                     /identity="97.16"
                     /coverage="95.56"
     ARG             3403..3704
                     /name="aad(6)"
                     /gene="aad(6)"
                     /gene_name="ANT(6)"
                     /tool="rgi"
                     /drug_class="aminoglycoside antibiotic"
                     /identity="99.0"
                     /coverage="36.23"
     ARG             10852..11696
                     /name="PC1 beta-lactamase (blaZ)"
                     /gene="PC1 beta-lactamase (blaZ)"
                     /gene_name="BlaZ beta-lactamase"
                     /tool="rgi"
                     /drug_class="penam"
                     /identity="96.8"
                     /coverage="100.0"
     Typing          complement(17913..18060)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="5.980000000000001e-72"
     Typing          complement(18119..18294)
                     /name="rep_cluster_1017"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.697"
                     /coverage="94.0"
                     /evalue="1.04e-65"
     Typing          complement(18426..19408)
                     /name="rep_cluster_2100"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.187"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          19835..20577
                     /name="rep_cluster_1733"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ttctaacgtg cgtaatgaag tcttacaaat tgataattac acgaatcttg cactgaacta
       61 tttgaaactc atgaaccaaa agacatacat ggtctttatg gaagtgagtg tagatgaatt
      121 aattcgacca ctgattaaac gttatgcgat acagtttatt catcatcaaa caaagttgca
      181 ttatgagaaa agtgaagcgg ttatattaac agatgccaaa tgggcatcaa ttatgattga
      241 gcaaatatta aacaatgcat taaaatatgc acgtggcaaa gatgtgtgga ttacgtttga
      301 cgaaaataca aagaaattat caatccgaga taacggtatc ggcattaatg cgagtgattt
      361 accgaaaatc tttgataaag gtttctcggg ctataacggt agaataagtg agaaatcaag
      421 cggtattggt ttatatattg tcagcacaat ctcaaagcgc ttaggccata aagtaagtgc
      481 tacatctaca ttaaacgatg gttcaatatt caccattcaa tttaaataac tccaatcttt
      541 catttttgta agattagcgg ctgtttttgt aagaatagat aaaggcagcc ttttctaatc
      601 gcgcgtaaga taaaggtaac aacaaactaa gaaaggtgcc agaggtgaaa aatttgaata
      661 atataattga aataaataac ttaacaaagc aatatggaag tcagatagca gttaataatt
      721 tgagtataac tgttgaaagt ggacaaattt atggtttttt aggaagaaat ggtgcaggca
      781 aaacaactac tataagaatg cttttaggct taattaaacc tacgaaaggt aatattaaaa
      841 tatttgaaaa agagttcgaa agtaataaaa ctgaaatttt aaaaaatatt ggtgctatgg
      901 ttgaaacgcc aggtttttat agtaatttga cagctaaaga gaacttaact ataaatgcta
      961 aaatattagg tatacaaaaa gaaaatgcaa ttgaagaagt gttagaaata gtaggactag
     1021 cttctgaaaa agtaaaattg tttaaaaatt attctttagg tatgaagcaa cgtttaggga
     1081 ttgcgagagc tttattgcac aatcccgaat tattgatact tgatgagccg acgaatggat
     1141 tagatcctat tggcataaaa gaaattagaa agttgattaa ggatctggct caatctagaa
     1201 atataaccat tttaatttca agtcatatat tgtcagaaat agagcagtta gtagacaagg
     1261 tgggtattat tcataacgga gttttgttag aagaaattat gtatgaagat ttaaaaaaaa
     1321 agaacagaca atttataagt atatcagtat cagatgaaga aaaagttgca tgtttgttgg
     1381 aaactaactt cggaatttat gactataaaa ttaaagaaaa tggcgaattt aaaatttatt
     1441 cacatattga tagacaacaa gaaattaata gattattagt attaaacaat attgatgttt
     1501 tccagatgag agtgatagaa gataatctag aaacttattt cgaagactta gtaggaggtg
     1561 gcacaattgg ataatctact ccgtacagaa tttattaagt taaaaagatc atatatgttt
     1621 ctaataagta ttttaggtac atgcgtagca ccttttatgg tggttgtagc ttcttttatt
     1681 agtatgcgga cgaaagtacc gactcctaat attgtattta aacagttatt tgaagaagtc
     1741 agtttatatc atatgctaat tattggacct cctctatatg gggttataat agcctatttg
     1801 tttggaagag aatatataga agatacttta aaaaacttat taattatacc tatatctaga
     1861 gaaagttttt tagtaagtaa aataatatta ttattcttgt ggattcaaac actatctgtt
     1921 attgcatggt tgctaacttt aatgctaggt atgctattgc attttcaagg gttaagccag
     1981 agtttaataa ttgattcatt ctttacattt gttattggcg gagtgttatt atggatatta
     2041 tctccaccaa taattatttt gtcgatatat tttaaaaatt atgttcccac aattgtttta
     2101 accataataa ttactttgat aaatttaatg actgctaatt ctgaacatag aggattattt
     2161 ccttgggctg cagttggaga catatctaga ggaacattac ctaatactta tccatttcta
     2221 ttatctttaa ttatcataat tgttacatct atattcagtg taattatatt attctcatac
     2281 tttaaaaaaa ctgatataag ttaaaaggag gagaaaaatg ggttttatag agcagattaa
     2341 agcatttatt ttaggtgtag ttgaagggtt gacagaattt gctccagtat catcaacagg
     2401 tcatatgatt attgttgatg atatgtggtt aaacactgct gacgatttag gaagtgcgtc
     2461 tgcaactact tttaaggtgg taatacagtt aggaggttct gttgcaaagt tgaatttata
     2521 atataatttt aacaaaaagg agtcttctgt atgaactatt tcagatataa acaatttaac
     2581 aaggatgtta tcactgtagc cgttggctac tatctaagat atgcattgag ctatcgtgat
     2641 atatctgaaa tattaagaga acgtggtgta aacgttcatc attcaacgat ctaccgttgg
     2701 gttcaagaat atgccccaat tttatatcaa atttggaaga aaaagcataa aaaagcttat
     2761 tacaaatggc gtattgatga gacgtatatc aaaataaaag gaaaatggag ctatttatat
     2821 cgtgccattg atacagaggg acatacatta gatatttggt tgcgtaagca acgagataat
     2881 cattcagcat atgcgtttat caaacgtctc attaaacaat ttggtaaacc tcaaaaggta
     2941 attacagatc aggcaccttc aacgaaggta gcaatggtta aagtaattaa agcttttaaa
     3001 cttaaacctg actgccattg tacatcgaaa tatctgaata acctcattga gcaagatcac
     3061 cgtcatatta aagtaagaaa gacaaggtat caaagtatca atacagcaaa gaatacttta
     3121 aaaggtattg agtgtattta cgctctatat aaaaagaacc gcaggtctct tcagatctac
     3181 ggattttcgc catgccacga aattagcatc atgctagcaa gttaaacgaa cactgacatg
     3241 atatattagt gattagctat attttttact ttgcaacaga accatcattt ttattaaaat
     3301 actcttttgt aaaatctttc tgctcttcaa tgtatttaat ttctttacct ctaaatctat
     3361 tatagctatt tgatgattac aataaaattg atcttacctt attgcccttg gaagagttag
     3421 ataattacct aaagggcgat aaattaataa aggttctaat tgataaagat tgtagaatta
     3481 aaagggacat agttccgact gatatagatt atcatgtaag aaagccaagc gcaagggagt
     3541 atgatgattg ctgcaatgaa ttttggaatg taacacctta tgttattaaa ggattgtgcc
     3601 gtaaggaaat tttatttgct attgatcatt ttaatcagat tgttcgccat gagctgctga
     3661 gaatgatatc atggaaggtc ggcatcgaaa caggctttaa atgaagtgta ggcaagaact
     3721 ataagtttat tgaaaggtat atatccgagg atttgtggga gaaacttttg tccacctacc
     3781 ggatggattc ctatgaaaac atatgggaag cattatttct atgccatcaa ttgttcaggg
     3841 cggtatccgg tgaggtggcg gaaaggcttc attatgccta tccggagtat gataggaata
     3901 taacaaaata taccagggac atgtataaaa aatacactgg taaaaccggc tgcctggata
     3961 gcacatatgc cgctgatata gaagagaggc gggaacagtg attacagaaa tgaaagcagg
     4021 gcacctgaaa gatatcgata aacccagcga accatttgag gtgataggta agattatacc
     4081 gaggtatgaa aacgagaatt ggacctttac agaattactc tatgaagcgc catatttaaa
     4141 aagctaccaa gacgaagagg atgaagagga tgaggaggca gattgccttg aatatattga
     4201 caatactgat aagataatat atctttacta ccaagacgat aaatgcgtcg gaaaagttaa
     4261 actgcgaaaa aattggaacc ggtacgctta tatagaagat atcgccgtat gtaaggattt
     4321 cagggggcaa ggcataggca gcgcgcttat caatatatct atagaatggg caaagcataa
     4381 aaacttgcat ggactaatgc ttgaaaccca ggacaataac cttatagctt gtaaattcta
     4441 tcataattgt ggtttcaaaa tcggctccgt cgatactatg ttatacgcca actttgaaaa
     4501 agctgttttc tggtatttaa ggttttagaa tgcaaggaac agtgaattgg agttcgtctt
     4561 gttataatta gcttcttggg gtatctttaa atactgtaga aaagaggaag gaaataataa
     4621 atggctaaaa tgagaatatc accggaattg aaaaaactga tcgaaaaata ccgctgcgta
     4681 aaagatacgg aaggaatgtc tcctgctaag gtatataagc tggtgggaga aaatgaaaac
     4741 ctatatttaa aaatgacgga cagccggtat aaagggacca cctatgatgt ggaacgggaa
     4801 aaggacatga tgctatggct ggaaggaaag ctgcctgttc caaaggtcct gcactttgaa
     4861 cggcatgatg gctggagcaa tctgctcatg agtgaggccg atggcgtcct ttgctcggaa
     4921 gagtatgaag atgaacaaag ccctgaaaag attatcgagc tgtatgcgga gtgcatcagg
     4981 ctctttcact ccatcgacat atcggattgt ccctatacga atagcttaga cagccgctta
     5041 gccgaattgg attacttact gaataacgat ctggccgatg tggattgcga aaactgggaa
     5101 gaagacactc catttaaaga tccgcgcgag ctgtatgatt ttttaaagac ggaaaagccc
     5161 gaagaggaac ttgtcttttc ccacggcgac ctgggagaca gcaacatctt tgtgaaagat
     5221 ggcaaagtaa gtggctttat tgatcttggg agaagcggca gggcggacaa gtggtatgac
     5281 attgccttct gcgtccggtc gatcagggag gatatcgggg aagaacagta tgtcgagcta
     5341 ttttttgact tactggggat caagcctgat tgggagaaaa taaaatatta tattttactg
     5401 gatgaattgt tttagtacct agatttagat gtctaaaaag ctttaactac aagcttttta
     5461 gacatctaat cttttctgaa gtacatccgc aactgtccat actctgatgt tttatatctt
     5521 ttctaaaagt tcgctagata gagttctata ttagagatgt aaagagtttt ggtgaagagg
     5581 tttgtgagta tggcttttat aaccagggtg caatgagaag cactaacagc agctagctta
     5641 agaacgcata ggatatactt tttatggaag tcacagagga ggaataaaat ttgaacagga
     5701 ttaacagagt aacctctatt cttattcagc tgcaatcaaa aaaataattc ctgccaaaga
     5761 aattgcacag cgatttaata taagcttaag gacagtttac agagatatcc ggacacttga
     5821 agaagccgga ataccaattg gatctgaggc cggaaaagga tattttcttg tagagggctt
     5881 cctacttctg ccggtaatgt ttacagcggc ggaagtgggt gcattgatta cagcagggaa
     5941 atttttaaat tgccatggag atgaatcatt tataaaggat tttgattcag ctatgtataa
     6001 aatcaaatct attttgaagc atggtgaaaa aaactatgca caggagttgg agaacagtat
     6061 taacgtgtac agcacatctg gacagaaaaa tacattggcg gataacgtta ttgcagcaat
     6121 tcagactgca atctgcaata aaagggtaat atcaattcaa tatcctgcat caggagggca
     6181 agaaccggaa agcagaatga tagagcctat atcactgggg ttttatgaac agaactggta
     6241 cttaatcggt tttgcaggct aagaaatgaa tataggaatt ttcgtgtgga tcggattaaa
     6301 agcatttgca tagaagaaga gctctgtcaa agccatgatg gctctttgga acaaatatta
     6361 aaacaaatgc tggttctgtt gcaaagtaaa aaaatatagc taaccactaa tttatcatgt
     6421 cagtgttcgc ttaacttgct agcatgatgc taatttcgtg gcatggcgaa aatccgtaga
     6481 tctgaagaga cctgcggttc tttttatata gagcgtaaat acactcaata ccttttaaag
     6541 tattctttgc tgtattgata ctttgatacc ttgtctttct tactttaata tgacggtgat
     6601 cttgctcaat gaggttattc agatatttcg atgtacaatg gcagtcaggt ttaagtttaa
     6661 aagctttaat tactttaacc attgctacct tcgttgaagg tgcctgatct gtaattacct
     6721 tttgaggttt accaaattgt ttaatgagac gtttgataaa cgcatatgct gaatgattat
     6781 ctcgttgctt acgcaaccaa atatctaatg tatgtccctc tgcatcaatg gcacgatata
     6841 aatagctcca ttttcctttt attttgatgt acgtctcatc aatacgccat ttgtaataag
     6901 cttttttatg ctttttcttc caaatttgat ataaaattgg ggcatattct tgaacccaac
     6961 ggtagaccgt tgaatgatga acgtttacac cacgttccct taatatttca gatatatcac
     7021 gataactcaa tgcatatctt agatagtagc caacagctac agtgataaca tccttgttaa
     7081 attgtttata tctgaaatag ttcatacaga agactccttt ttgttaaaat tatactataa
     7141 attcaacttt gcaacagaac ctcatttact atatgggcat ctctttcatt attaatacaa
     7201 agctttgtat ctccgatatt aggcaaaggt gtaaacgaat taggtgatac tataggattc
     7261 aacattatat taatcgtttc tttaataatg attatcttct tattttttat aaatcgtagt
     7321 aaaaatatct cctatgatta tataaaagtt caaagaggtg aagccaatga ataaattaga
     7381 aaaatggtct ttggtattta ttatttttac atttctctct tcattagcac ttttatattt
     7441 caaatataat tttagttcgg ttatgccagt cctaagtctt tctattttaa ctatactaat
     7501 attgttgacg agtattatta ctgtagtttc agttaatgat gaagatactt aaaacgttca
     7561 ttagtgaaac aggtattata ttaaaaaagc gtacgaattt gtacgctttt ttcttgtgtt
     7621 attattaaaa tctcataaca ggaaaatatt atgagatttt agacgctgtc agaacgtctc
     7681 ttttacatcg agtgacattg aatcaaagtc tttgtatgtt aatgatatat aaaattattt
     7741 agtagattca ttatagtaag ctagattagt tgctctactt gtttttattc tcttttgcct
     7801 ctaatttatt gagataacga taaatagttg tacgtgaaac ttgccactgc tctgcaattg
     7861 ttttaattgg cgtaccatta tcataaagtg tcttaagtaa gtttaaatct tgtttattta
     7921 atttttcagg tctacctcca tatcggcctc tagcacgtgc tgcaattcga ccggctgatg
     7981 atcgctctaa aattaaatta cgttcaaact ctgcaaatgc tgcgaataaa tgaaataata
     8041 attgtcctgt agaacttgat ttatccatcg ttatattttc ttctaaacta tgaaaactca
     8101 cacctcgttc gttaagttca ttaactaacg tgattaaatc ctccatgttc cgtcctagtc
     8161 tatctaatct ccaaacaaca atcgtatctc ctgacctcgc aaattcaatt gctttatcta
     8221 aaccaggtcg cttacttttt gaaccactca tatggtcatt aaatatcttt tcacaaccat
     8281 atgtatttaa tcgatcctcc tgtaaattca aattttgtaa tccagttgaa actcttgcat
     8341 aacctatttt caaaatacag acctcctata tacaccttta gtgtaccata acttgattac
     8401 gaggtctttt gtgaacatag attttttgtt gggtttttga acacataatt ctatgtaata
     8461 cttacttatt tagacaaaaa acaagagtgt acagaaaatg gttattttct ggacactctc
     8521 atctttaata atatatttta aatgtcatta acaaaactaa tttaataaga gtcaagtatg
     8581 gtttacatat gtattatgtt tgaaacatac ctctaattac tttttactaa tatcatttaa
     8641 aatgtctcgc aattcttcaa tttctttgtt atttaattct tcatttttcg caaaattcag
     8701 cactaaactt ttcatatccc ctccatacag tttattaaga aaggttttag cagttttcat
     8761 tttaatatcg tcttctttaa tatttgatga gtaaaaataa atattttctg atttgtatcg
     8821 ttttataatc tcttttttat atagtcttgt gattaatgtt ctaatcgttt tatcactaac
     8881 ttctttatat ttttgaattt caactacaat ttcattagct gatactgatt ttttatccca
     8941 tattatattc ataacatccc attcagccat agatatttca acttgcttat tggtcattta
     9001 aaacacccat tccttttaat attttttcac ttattaattc agcatttttc ccagacgggt
     9061 ttccatctga taaatgtgta gcaaaataat acttatcatg atttgtaatt acgtaaccta
     9121 caaaccaccc attattatac ttcccgttta ctatacctgt acctgttttc ccatacagtt
     9181 catacttttc atttttctta atcaataatg aagaagataa ttgattcttt gcttttttac
     9241 taaaatggtt attttgttcc atcatatttt taaaaactat tacttgttca agattagata
     9301 ttttcaaact atcttccatc caatagcttt tataacttcc caaattttca ttaccataat
     9361 ttaattgctt gagttgagcc gcagtatagt tctttggtat ttgattacta atacgttcga
     9421 agtaccaatt aactgaattt tgcattgctg tatttaaatc ttgttcctta ttccaagact
     9481 cgaaaagata atgtttatga ttccatgaca tacgtgaatt tttatcactt ataatatgac
     9541 ggtcaagtcc aaacagtgct aaataaattt tgtaagttga atcaggagaa tatctttttc
     9601 tactttcttt ttcattataa atatagtatt tgtctttctt catgctgtac ataacgaaag
     9661 atccactatt tgaaccaaaa tttttacttt catctaaaat ttggtaatcg ctttgtaagg
     9721 gctttttata attataatca gttaaggatt gccccattaa aaactggctt tgaattatca
     9781 tgataaaaaa agtaaaaata caaataaaaa ttagaattaa ttttgactgc tttttcagat
     9841 cggcttcttt tatattaatt aatctacttt tgagtaatga ttttttacca ttaaatgaat
     9901 ggcttaatat atttttgttg gaaaacgggg tttttaatac ggagtccata atagattccg
     9961 cataagtttt aaattcattt ttgttaatat tattaaggac aaatctatcg gcttctactt
    10021 cattgtcatg aattatcttt ctttttacaa tatgtactaa tggattgtaa ctcataataa
    10081 tagagaagat attaaagata attaaatgta aagtatctct atttttagca tgagcatatt
    10141 catgcagtat tatatatttt agtcttttat caattacact tttaaaatat gaactaggaa
    10201 tcaaaataat atatttccca taccaaaaag ttattggaga ttgaatagcc tctgcttttc
    10261 gaatcacaat atttttttta tattgatggt tgaaaagtat cgtatctatt ttattttttt
    10321 cattttcgtt tagataaagt gactgtttct ttaaatattt aagatataat aaggctttca
    10381 aaaatttaaa acttaaaata ataactaaaa ctatccaaat aactgtgcag atattatcaa
    10441 ttgaatccca attaaactta tggatatctg ttgtgaactc ttgaataggt ttggtggtat
    10501 ttatgttatg gttcaaatcg tgtgacttac tttcaactgt gggcgcttga ttattcacat
    10561 tattaaattt tataaaagag aatttaattg ggataaaagg aattaatcca gcaagcatag
    10621 ttagatacca aactttataa tttaaactat aattaaaata gcgttttaat atgtacctaa
    10681 aaaatactaa caatagaaat ataaaacaaa agcttactac gctcattatt agtaatttag
    10741 ccattttgac accttctttc aaatatttat aataaacaat tgacatcaat attacaattg
    10801 taatattatt gatttataaa aattacaact gtaatatcgg agggtttatt ttgaaaaagt
    10861 taatattttt aattgtaatt gctttagttt taagtgcatg taattcaaac agttcacatg
    10921 ccaaagagtt aaatgattta gaaaaaaaat ataatgctca tattggtgtt tatgctttaa
    10981 atactaaaag tggtaaggaa gtaaaattta attcagataa gagatttgcc tatgcttcga
    11041 cttcaaaagc gataaatagt gctattttgt tagaacaagt accttataat aagttaaata
    11101 aaaaagtaca tattaacaaa gatgatatag ttgcttattc tcctatttta gaaaaatatg
    11161 taggaaaaga tatcacttta aaagaactta ttgaggcttc aatgacatat agtgataata
    11221 cagcaaacaa taaaattata aaagaaatcg gtggaatcaa aaaagttaaa caacgtctaa
    11281 aagaactagg agataaagta acaaatccag ttagatatga gatagaatta aattactatt
    11341 caccaaagag caaaaaagat acttcaacgc ctgctgcttt cggcaagact ttaaataaac
    11401 ttatcgcaaa tggaaaatta agcaaaaaaa ataaaaattt cttacttgat ttaatgttta
    11461 ataataaaaa cggagacact ttaattaaag atggtgttcc aaaagactat aaggttgctg
    11521 ataaaagtgg tcaagcaata acatatgctt ctagaaatga tgttgctttt gtttatccta
    11581 agggccaatc tgaacctatt gttttagtca tttttacgaa taaagacaat aaaagtgata
    11641 agccaaatga taagttgata agtgaaaccg ccaagagtgt aatgaaggaa ttttaatatt
    11701 ctaaatgcat aataaatact gataacatct tatagtttgt attatatttt gtattatcgt
    11761 tgacatgtat aattttgata tcaaaaactg attttccctt tattattttc gagatttatt
    11821 ttcttaattc tctttaacaa actagaaata ttgtatatac aaaaaatcat aaataataga
    11881 tgaatagttt aattataggt gttcatcaat cgaaaaagca acgtatctta tttaaagtgc
    11941 gttgcttttt tctcatttat aaggttaaat aattctcata tatcaagcaa agtgacaata
    12001 gcattttaca agtatttttt attgttcgat tagggtaaac ctaaataata cataaattat
    12061 attacaatat gtttattata gaggctaaag tatcttagaa atttggatag taggtttttc
    12121 gggaataaaa gtatagttgg ctttaatata taattaagct aagctatcat ttatatttac
    12181 tcgattataa ttcgcacttc aactttttta taacgtaaaa attaaaacgt agttttattt
    12241 atctttagta atatcttttt aaacactaca gtttcttcat tagttaaatt agcgaagtaa
    12301 tgattattaa gttgataggc tttcttttct aatgttggcc ataagtcttt tgctttattt
    12361 gtactgttta ttagcacagt tcttctattt tcattatcag tttgacgtgt tattagatcc
    12421 tttttggaca tcttatctaa tattccagat attgttgaag gttctaaatt taaagtatta
    12481 ccaatcgctt taggtgtatt caaattacta ttaatcaagc aattcaaaac accatattct
    12541 gctggagtaa tattataggg acttaataaa cttttaaaat gtttaaaaac tctattctga
    12601 gaagtactca acaaataatt tatacaatta tctaattcca caactaaaac ctctcttaaa
    12661 tcattagtat tgactcgctt atcatatcat gataagtttt gtaggaaaat agttttgcaa
    12721 caaaaacata cttctacaaa acaattgtga aagaaggcta ataaatgttc aatgcggtag
    12781 tagtgagaaa aagtgcatca aatgttagtt ataaagtaga aaaagttgat gaatctcatt
    12841 tatcgaaggg tgatgtatta gtaaaagtgg tatactcttc aataaattat aaggatatgc
    12901 tagctttaca atataaaggc ggtgtaatta gagattatcc tatgattcct ggtatagatt
    12961 ttgcaggtat tgttgaatca agttcgaatg ataagtttaa agaaggcgat aatgtattag
    13021 ttactggtta tgatgttggc gtgacacata caggtggatt ttcagaatat gcacaagtgc
    13081 ctggagagtg gattgttcct attccccaag aaatgtctat taaagagtct atggtatatg
    13141 gcacagccgg ttttacggca gcattatcaa tttttgaact agaaaaatat ggaatgaata
    13201 tcaaatcaca acctgaaatt ttagtgtctg gggctacagg tggtgtgggc agtgttgcta
    13261 tacaaatttt acataaaatt ggatatgaaa atatcactgc tatcgtaaga aaagaaaagc
    13321 aaatagaaat cgcaaaaaaa ttaggtgcaa aacaagtaat aataattgaa gatgataata
    13381 aaccgcttaa aaaaggtatt tttgattatt tcttagatac agtggggagt aatgtaacat
    13441 tatacggttt aaaacgtcta aattatcaag gtgttgctac tgtatgtgga aatgtagcag
    13501 gaaattcact taacgctaat atattaccgt ttattcttag aggtattaaa ttaattggca
    13561 ttgattctgt atatgttgat cataatatcc gtgaagatat atggagtaag ttagcaaatg
    13621 aatggaatat aggaaattca cttctataca atgaaattgg atttgaagaa ttctataaaa
    13681 cgatagatca gcttttaaaa ggacaacatt taggaagaac tattttaaaa gtataagtgt
    13741 gagtattcat taatacattt actattaatt caatcatttt gcatttctct actaaccagt
    13801 gttataattt atataacgaa aatgattaac aattaatgca ccaatcccca cactattgca
    13861 gtagtgtggg gattttttcg atgctggctt ataaagtcgc ctattaatta tttgttgcgt
    13921 ttactaagcc aataagtata atacgcaaca gcacagccac tgatgactgg tgctatgatg
    13981 tgaacgaaaa tgattagcaa agtaatgcac ctcctctctt cgtcaattga cgcctgagag
    14041 ataggcgact ctattattat accatctgtg aatatacaag catatctact tccgataagt
    14101 cgtcttatgt tatataactt tgttttttat agtatcaaga caagaagaaa ctcgtttcaa
    14161 aaaccctcat gatttttaga gaaaaactct cttatttcat cgtctttaat aaaataatat
    14221 gccatctttc catctttata aaaatcaagt acctcatttt tatataaaag ccttaaatga
    14281 tgtgaagttg aagcaacact cattttcaat atcaaagaaa tatcacaaac acacaactca
    14341 tcttctttaa tcaaagataa tatgattttc aatttcttct catcacaaat tttttctaaa
    14401 atgttaagta atttcttaga tttatcatct tctaaaaaac ttaaagcatt gttaacttta
    14461 tcctcatgta cacagatcac atcacaagta ttttcataac tcatataaaa tcacacccta
    14521 aattaaaata taaatcctaa aattgtttga atagtgtcat tttcaataat aataaataaa
    14581 cctaaagcta tataaataat agccataatc caacgactaa atttctcaac aatttctcca
    14641 attcctggaa tattagctaa tttttgtgca gtaaacacca agaagaaaat taaaattaaa
    14701 aagacaaaca gagtaagtaa taaattagta acacttaatg tcacaaaata cggaacaaat
    14761 aaaccaatat tatcggcgcc acaacttgct atcgtaacaa ttgcaaccgt accaactaat
    14821 ttagacaatc ctttttcatt caattctttt ttagctctct tttctccttc acaatcatca
    14881 taaatagcca ctttaattcc aagataaatt ggtattaaac ccaataaacc taatatccac
    14941 ttttctggaa cataatttaa tacaaaagct aaaaacaaac taactaatat taaaataata
    15001 gaccctaaat attgaccaac ataaatatct ctatattctt ttctagtctt tgctctagca
    15061 aaaaatatta ataaaattac taataaatca actgctgtag caatataaag aacagcagca
    15121 gcaacaaccg tttgaatcat aatacacctc attcaaaaat atttttgaat gtattataac
    15181 ttatattttt aaataatcat aggtgcttta ttccaattgc tttattgacg ttgagcctcg
    15241 gaacccttaa caatcccaaa acttgtcgaa tggtcggctt aatagctcac gctatgccga
    15301 cattcgtctg caagtttagt taagggttct tctcaacatc aataaatttt ttcggcataa
    15361 atgcgtgtct atgcgtagac atggagacac ttcagaatta tggagtgatt acatgagttt
    15421 aactattttt ctttattcag ttttaggaag tttattagca aattatatct acgaaaaatc
    15481 ttcaaaataa ataacttttt cggttctact ttgtacactt tatctacctg gtagacctaa
    15541 ttgttattgc tttacaggtc taaaattcgt cgttataatc tagttaaatc ccgtaagggc
    15601 gcacttatac gtcggataaa tcgacatgtg caaaaacgta atcgaccttc gtcgattttt
    15661 ttatttcaaa attaaagaaa cggcttgaga tattccgagc agttataaaa attaatttgc
    15721 gttgaatatt gctactacat tcttgaaaaa gtgatgtaga tttatagccc ataacttttt
    15781 tgaattacat cgacgatacg gcgtcgaaag aaaaataaat agaccgtatg aaagaaaaag
    15841 ctacctcact ttatctgggg tagcttttat tttttatgcc cttattttga attttaagag
    15901 ggcattttaa agatttaggg gtgattcata tagttttata tctaaaactt atatatgctt
    15961 ttaaaacgca aatatgagcc aaataaatat atgctatttt caaaaacaaa aatttgagca
    16021 aaaacaataa ctatttttag acactgccca tttacatgca aattaaaaat tagcataaaa
    16081 ttctttaaaa cttccccacg tttttctaaa ttcctgtagg gcgtttttat caaaaatcaa
    16141 gacaagaaga aactcgttca cactcgtttc aaaaaaacaa gaaatctaat ctatacacct
    16201 accgttttcc aaccccctca aagcatacaa attaaaaaca aaattattca taatcttact
    16261 ttcttttaac aaaaacataa aaaccataaa tagacaaatt aactatcaaa ggcaataaac
    16321 agtataaaaa aaccacgaat aaatcttcat caacatctaa aataaaaaag aataaaacaa
    16381 ttaacaaaat actaattaac aaacctaaaa gtggcaaact ttttatatat ttttctaaat
    16441 tttcattcat aaaatagact cctttcaaaa atatgtttat aaatatatta aaataaatat
    16501 tgattttata aagcgcttac ttatacaatt aaaatatctt attatcttgg aggtatcaaa
    16561 tgaaaattat aaaattattt tcaataattt ccttgttcct actggtattt agcgcgaatt
    16621 tttctaatgc ttacgcaaat gaagaacaaa aaagttcttt actagaaaat caaaaagaaa
    16681 aattgaaaag caaaaacatc gaagtatcac aaatgaccaa gaaagaaata agttttctag
    16741 aacaattgca aaataaaaat gaagatatgc aaaaattcga aaagcaaatg aaaaaagaag
    16801 gatttaaaaa aataaagcta gaagacaaaa attctaaatt aaaatatcta gctacagaag
    16861 caaatgataa aaatgcatat ggctacatag ctaataacgc ttataaaaat gaaaaaacaa
    16921 aagaaattgt agtttctgaa acaatatatg ataactacaa caaaaaaatc acaaaattca
    16981 ttgccgaaaa aagaagtatg actgaaaaca aagagggaaa aatacttatt aacaaaaatt
    17041 acgttgaata taaggctgaa aaagttgata aaaacagacc ggtaacatat gcaggattca
    17101 aatggaacgg caaagcattc gcttgtagta tggctggact ttatgcatgt gctcactact
    17161 gtggagtatg ggcaatcgta aatccaatag ccggcggtgt atgtgaagca gtttgcggaa
    17221 ctgcatttgc agctggatgc tctgtaggtt gattttattc caattgtttt attgacgttg
    17281 agcctcggaa cccttaacaa tcccaaaact tgtcgaatgg tcggcttaat agctcacgct
    17341 atgccgacat tcgtctgcaa gtttagttaa gggttcttct caacatcaat aaattttttc
    17401 ggcataaata cgatgttcct tatttatcct cataaaaaac aatagctgaa atatataacc
    17461 aattactgtg tgtttgaatt tctaatacat gaatatcatt ctttaaaaac ctattaactt
    17521 gtttatctaa ttgacgatga gtaaaagtag cgaaagtttt aatcttcatg atatctctcc
    17581 ttttataaaa atagcaatcc ttattacaat attaaacact tttattttgg attttaagag
    17641 gcattttgaa gacttagagg caaatcatat aggttttgta cttaaaaatc tatacagtct
    17701 cttaaaacgc aaatatgagc caaataaata tatgctaatt tcacaaacaa aaatttcagc
    17761 aaaatcagtg actaattttt tagatactgc ccatttacat gcaaatttaa aaattagcat
    17821 aaaatacttt aaaacttccc catgtttttc taaattcctg cagggctttt cttatcaaaa
    17881 acttagaaat tttaatttaa ctctaaaatc tccgtaagag cataagggaa atttgctatc
    17941 attcaaaaat gatagatctg gcaaggccag acattccaag ggttttaagt tattttaaag
    18001 tgatatcata caaagctgta gtgtcacact tttgtgacac tacatacagc atttgtcaca
    18061 aaaataagac agcacttttt tacagaaaaa caataaaaaa agacatcagt tatcactaat
    18121 gtcttaaata ataattttgc ttttcgaaat tccaaattgc ggtaattgaa tgtgcttttt
    18181 ttcttcgtct gtcgttttat cttcatcact tgtattaatc aaattaccat cttcggcatc
    18241 gtctaagttt aaaattttat gcttttgttt aagcaatcca ccataactta acatacgttt
    18301 tcgatacaaa cctttttcta aatcacttac aatttcttga tttttttcgt cgtcatcagt
    18361 taaaaagtca gatgatttaa cggaatattt tgatgtctct tcgagtgtta taaaatcatt
    18421 tgaattattg attaaagata ttatattgtt tcatgttttt acggcgcata taaagggcat
    18481 gtgtttcttc aagttcagtt agaattgttt gcattaaata agcttgcatt gacttgattg
    18541 tttccttttg gcctttaact cccctatcat gtatagcttt tttaagtctt tttaaagaag
    18601 ttaatatttc atcctcaatg tcctctaaca tgtaatgcat atcttcatgc cttgaattaa
    18661 atgatttttt tgctttaaga ataacagcct ttatgatttc taaatctcta taatcatatt
    18721 ttaataaata attagttagt tgttctggga attcttgcaa tagcatttct ttatcatcaa
    18781 aattatgtgt aaattgattt gaatgatttg aatgattact aatttcattt ttgtacttta
    18841 tatcattcaa gtcattcata tcattattct cagtatcatt aaagtcaatg tcattaaagt
    18901 cagtatcatt agtgttcgat tttcgaactt ctggacgttc gattttcgaa cttctggaag
    18961 ttcgaaaatc gaacttctgg acgttcgatt ttcgaacttc cttctcgccg taaggttcaa
    19021 catcgttttc ttctttttct attttgtaaa tatcacgttc tgtgacaata ggtttcatta
    19081 aatatagaat atttggttta tttaaaccac gtcgtttttg aatgagtaga ccatcgtttt
    19141 ctaacccttt ttttattttt gttaactttt cttttttaca atttaagatt tccattagct
    19201 tttcatttga ataaacaaag taaatgttac catcttcatc aacccagcta tttttaatag
    19261 ataaatttaa gcgatcacgt aagatagcat aggctatttt catatcatta gttagatttt
    19321 tataattttc actggtaaag aatactttag ggatttgata aaatctttct ttgtaatttt
    19381 cttgcactgt aaaatattga ttttgcatga caaaaaacct ctttccatat tttagtttgg
    19441 taagaggtaa taaaacgttt ttatttagct ttacatttat ttaatgattt aggtgcttgc
    19501 actaagatat tattcattat ataatacaag tatcgaaaat tatataaata aacagtttct
    19561 aaacgtttta ttaccaaacc ccgactatta ccagtagttg gggtttttgt tatattaagt
    19621 ttattatagc actttaaata aacttataaa agggaaaata tagctttatc aagggtttat
    19681 aaacccttga taaatatcat aaacctttca taaacccttg ataaaaaggt tgatggatgt
    19741 ttatttttat ttattaatca ctttattatt aagcattata agcttataat attttatcaa
    19801 gggtttataa acattttata aagggttgat gatatgaaaa gtgtgaaaaa gttgagtgaa
    19861 gaattagggg ttagtaagca aacgattttt aataatatta agcgattgaa tatagagaca
    19921 ataaaacagg aaaacacttc gtttattaaa gaagataccg atatagaaaa aatcattcaa
    19981 agagtaaatg aaaataaaaa gaaatatggt tttgaaagta ctactgaaga caaacaaaaa
    20041 aaagggtcgg ataatattaa ttttgaatcg aaaagcgatg ctcaaatagt agaaatcctt
    20101 aaaaatcaga ttgatacttt aaataatcaa attgaaaaac aagaaagtcg tcatgaaaca
    20161 acaattgaat tttacagaaa agagttgcaa gaaagatcaa aattgcttga aaatcagcaa
    20221 gttctggcat tagagagtaa taaaaagatt caaaaattag aaaatcaatt agaaaaagaa
    20281 aaacacctta attatccctt tgatacatct actaacacta gacaaaacgt tgatgcacaa
    20341 gaaaaaacat atactactaa tcctgtaaat attaacagag atcaggaaga aactaaagaa
    20401 acagagattc aatataagga tatatctgga agtcaaagcg acgaaagtac acaaggtgaa
    20461 gaagcaaaaa aagaagacgt tccagctaat ctaaatgaca atgatagtga tatagaagaa
    20521 aagagtgaag aaacggaacc taaaaagggg ttttggagtc gtttgtttgg tagctaaaat
    20581 aattgattag gcactaaaag ttataaaatt ttagtgtctt ttatatagtg agaacttctg
    20641 gtaatcatcg attatgtaaa ctaaagtatt tcaaataatt aactgtatta atactctcat
    20701 catgattgcg ttataaacgt tcgttattga gacaaaattt aatagtacta ctacacaaaa
    20761 agagagtgcg aattcgcact ctctttttgt gataaatgaa tggttaagaa aataattcat
    20821 ccacagttgt atcaaacaat cttgccaact taaatgctaa tgtcaatgta ggatcatatt
    20881 tattgttttc tattgcatta atagtttgcc tagacacttt taattcttta cttaaagttt
    20941 cttgagaaat tcctttttgt tttctatatt ctctaattct atttttcatt aatttaacct
    21001 ttagatgtat attttttatt tgtagtatag taattaaaca gcataagaaa tagtgctaca
    21061 attataaaga aaatttctgg atattgagaa aaccaagatc cttcaatact tggtgacaaa
    21121 cctgtaaatt taagcactag tgtaaataac atacaatata caatccaaat aaaactttta
    21181 gccagtattt caataaacat tttacgttgt ctttcatcgt atcgatcttg ttgtcctttt
    21241 ttatctaatg tttctgaagg taaatatttg ttagttatag ccgtccataa acaagaaaaa
    21301 attaaaataa taataaaagg aattaaattc attataacac ctcataaatg taaaaagtat
    21361 ttgacattta tgagtgtaaa tatttttgat acaaatgtca aatacttttt acattataat
    21421 atattttaaa acaccttatt agtaaaacaa ttactatata gaaagctgcg aactcgtact
    21481 cttttttctt gtataattat taaaatctca attcaataat atgttatgga atttgagaca
    21541 ctgtgagaga cgctcatttg cattgacaat caatttatca agataatccc ttatcatgtt
    21601 taattctata aactgtttgt cttgtgatat taacttcttt cgcaatctta ctaatcgctt
    21661 tgccttcttc taacatttcc accacgcgat gataaataac acgtttctgt gggtctttag
    21721 catttggtga ataaagtaaa gggcgccctt tatatacgcc tttttccttc gcaacttgaa
    21781 tcccttgcgc ttgtcgacgt ttactttcat ttctctcttg ttctgaaacc attgctaata
    21841 tttgaatgat taaatctttc ataaacttat ccagtaatgg attgccaatt acttcattca
    21901 tcataggtaa gctggtaatc attaactgaa cgtctttttc ttttaaataa ttcacagttt
    21961 caataatttc atcatagtta cgtcctaaac gatcaataga ttccacaacg aatcgatctc
    22021 ccattctgac aaaattgagt gcttcttgaa atacaggtct attttccact gactttcccg
    22081 actgtttctc tgtaaagatt ttctccacac caaacgtctt taaattatcc aattgtcttt
    22141 ctaaattttg atctatcgat gatactctcg cataacctat aatcattcat cttcactcca
    22201 taataaatat acaccctaat catacgtttt aattgttata atttcaacaa ttctttttgt
    22261 atggttaggg tgtactcaaa gtgtacatat ctataattaa aacttactaa aaataaatat
    22321 agattttttc tttgtaacag aaccgttcat ttgaaggtca tctctatcat aaatatttaa
    22381 attaactaaa aattttaatc atctgtttaa tacttatgaa atgtatttac ggtaggtatt
    22441 aaaattgatg aaatttatat aagtgctttc aatgaaaatg accgtttatt tatcctcctg
    22501 atttaaggag ttagaaattt cgaaaacgat tctttccatt tgttgcattt gttccatgtc
    22561 cagatattta atacattata gaaaaccaaa gaccatcact aattaaacgc agttttaaaa
    22621 tttcatcagg attgccttcg tctttaaaat gggcctcttt taatataagt tctactcttt
    22681 catacctaac tctttcaatg ccatcgtttc atacgccgat tctcctgatt ccatagcaaa
    22741 ctcagcgata aacattggga atactgcatt gagttctatt atatgctctt tcattcgtgg
    22801 ccatatttca cctccagctt taccgtatgc tgttataagt tgctctaatc cttcgtcgtc
    22861 gaatacacga tggtgtccta taaagtccat tgatgggtcg gagtgggttg cttcagtcca
    22921 gtctatgaga cctgtgacgt ttgcttggtt atctaccatt atatgtcctg gatgtaagtc
    22981 cccatgtatc atggtcgcat gtcgaggcca tagttcgtcg ttttctaacc attgtttcca
    23041 tctgttccat aattcatctg atacgccata agtttcttta actttattca ttcttctttg
    23101 aaagtcattt tttatttctt gtatggtttt gatatttata tgctgaacgt taatgttttc
    23161 ttctggtatg ttgtgtaaat ctacgagtgt ttcagctaat gtgttaataa agttttctgg
    23221 taatggtttg tgttcaattt cccatacata attttgtatt tctggatcta ttgtggctgc
    23281 gggtttacct gtaagttttg ggtaagcaat aaggtctttt gcatgtactt tccacttcgg
    23341 tatttcaaat gaaacattct tctgtaagaa gtctaccgtt tgtttttcgg gttttgttcg
    23401 tttataaacg tcaggtcttc ttggtagtct caaaacccat tctactccat gtttatcttt
    23461 tccaaatgca acttgaaaat caagtcccga ttcattcaat gagattgttt gaggttggat
    23521 gtgtaattga tatttttctg cacatttaat aatttcatta tgtcgagtca ttttattgga
    23581 tccttatttc tattatattt ttcttgtgaa cgtatttttt atattgatat aaatgaacct
    23641 aatattcaat ataaattagt gtatagtttt taagttatat catgaagaga ttgtcctgtt
    23701 aattcccatt ttttatctga tacatgttta acaaaccttg tatcatgtga tgtaaacaaa
    23761 atgattccag gatatttatt cataaacatt tctaatgctt ctaatgtttt aatatctaaa
    23821 aaattagttg gttcatccaa aattaacata ttcgctttcg ttgaaaataa tactgctaac
    23881 gataatttcg ttctttcccc accactcaaa acattacaag aacgatcaag tgcttcattt
    23941 aaacccaagt tatttaaaat agctctactg aatgattctg atgaatccgt ttcatccatt
    24001 aaatattgca ataatgaaac gtcacgcatg tcttcataag caagttgacg atagtatgcc
    24061 atttgcactt taggagaaca atcaattccc tctatttggt ggtaaatagc ttcaagtaaa
    24121 gttgtcttac ctacaccatt tgcacctacg agcgctatat ttttgccata tggtatttgg
    24181 aatcgtactt gtgttagcag tttttgactt cctttaacca atgttagatt ttgtgcaatg
    24241 attggataat tattatggat atcataaatt ttattttgtg ggaaattgaa ttcatgataa
    24301 ctttgtggtt tttcaacttc ttccaaatgt tccattcttt tttcaatatg cttagcttgt
    24361 ttttgagcag ccttctcaac cgtgcctttt tgttttgatg cacttaaacg atctggtgct
    24421 atacttttgt ttttttgttt tgatgatgct tgtgccattt gttgcgcttg atttcgttta
    24481 gctttactgg cttgggacaa tctttgtttt tcacttatat actgttcata ttgacgttgt
    24541 tgttctaact gttcttgttc atattgattt tgatactgtg tgtaattacc tttaaacact
    24601 cttattgtgc catcttcttg tatatcccaa attgtgtcag caatttggtc tataagtgct
    24661 ctatcatgac ttactataat taaagtacca tagtaatatt ttaaaatatt tttcagataa
    24721 tctttaccaa ttttatccaa gtgatttgta ggttcatcta aaagtaatat cggactataa
    24781 tttgatatga cattagctaa tttatatttt gctttttcac caccactcat actgtcggtt
    24841 gtatgcatag gtatatggag ttcactcatt aaactaccgt ctaacgtgtt aaaatcattt
    24901 tccacatcca tgttaagctg ttcaaaatat tgaatttcgc caaccgtttc aactgttcca
    24961 gattctggtt ttgttttttg agcaatcata tttagtaatg tggttttgcc agtaccatta
    25021 cccccaataa gtgctatttt ttcaaattgg taagcgtaaa gatgaccgat gttaagtgat
    25081 cgtaaatctg tcaatttatg attgatttgg ttaaatttaa ttgtatattg ttccatattt
    25141 atacactcct tgtatattta aaggacgtgt acgcaaaaaa atgagacagg ctactcccgt
    25201 ctcattcaca ttacactttc aaacacttaa aaatttatta tgatgtttta gtatttgaaa
    25261 gcgtttatta ttcctatcga aggataaaaa atgacataag tcatcctatg agtgtgtatt
    25321 gcggacaagc ctttttgaat taatttagtt ttgtattcaa aaaggtttat ttgagtctca
    25381 tagaagctgt cattttaatc ctccttcatc gataaaatag taatgttaat ataacatttt
    25441 gttggttttt ttacaatata tctctaagta gaaagattat ataacaaaag tataaaaata
    25501 caattttaga ttgattatac atctccttaa tttagagtgt actcataaaa tacggttctg
    25561 ttgcaaagtt gaatttataa tataatttta acaaaaagga gtcttctgta tgaactattt
    25621 cagatataaa caatttaaca aggatgttat cactgtagcc gttggctact atctaagata
    25681 tgcattgagc tatcgtgata tatctgaaat attaagagaa cgtggtgtaa acgttcatca
    25741 ttcaacgatc taccgttggg ttcaagaata tgccccaatt ttatatcaaa tttggaagaa
    25801 aaagcataaa aaagcttatt acaaatggcg tattgatgag acgtatatca aaataaaagg
    25861 aaaatggagc tatttatatc gtgccattga tacagaggga catacattag atatttggtt
    25921 gcgtaagcaa cgagataatc attcagcata tgcgtttatc aaacgtctca ttaaacaatt
    25981 tggtaagcct caaaaggtag ttacagatca ggcaccttca acgaaggcag caatggctaa
    26041 aataattaaa gcttttaaac ttaaacctga ctgtcattgt acatcgaaat atctgaataa
    26101 cctcattgag caagatcacc gtcatattaa agtaagaaag acaagatatc aaagtatcaa
    26161 tacggcaaag aatactttaa aaggtattga atgtatttac gctctatata aaaagaaccg
    26221 taggtctctt cagatctacg gattttcccc atgccacgaa attagcatca tgctagcaag
    26281 ttaaacgaac actgacatga tatattagtg attagctata ttttttactt tgcaacagaa
    26341 ccagccattt aatatgactg ttacaattac taagattcaa gcactattac gccgcacgta
    26401 tgatttcacc actcaagtta ataatttaac agtagatgat tgcgagttac tacttgatgc
    26461 agcaaagctt aaatttaatg aagaaaccat agacctgaca cacactgaat tgcaaatcat
    26521 gcatagctta ttccagaaca agggcaaatt cgtcagtcgt aatgcgttaa tcgaaaaatg
    26581 ctgggaatca gaacacttca ttgatgacaa tacgcttgct gtcaacatta cacgtttacg
    26641 caaaaagcta caaaaactag gacttacaga ttttatcgaa acgaagaaaa atgtcggata
    26701 tcgtgtaggt gacgtatgaa tttcttgaaa tcaatccaac gtgaaattta tattgtcatc
    26761 cttacttgtg ctttattcta ctttatatta ttcatgtata atgtgccgat gcaagcatat
    26821 atgattggtc taggtattgt attgtttgtt tcaatgattt actggggtat ccattacatg
    26881 aactttaaaa aagaataaac actacaggaa cagaacgaag atttaagaat cgaaaaccat
    26941 aaacttcgta atgactttat taactaccaa agtgagttag agtcatattt tttaatgtgg
    27001 gtgcatcaaa tcaagacacc gattacagca tcaaagttac tgcttgaaga tcctgatgaa
    27061 cacgtgat
//
