LOCUS       NZ_CP031857             2310 bp    DNA     circular CON 17-MAR-2024
DEFINITION  Actinobacillus pleuropneumoniae strain 16287-1 plasmid pAP17_1,
            complete sequence.
ACCESSION   NZ_CP031857
VERSION     NZ_CP031857.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN09909036
            Assembly: GCF_021953265.1
KEYWORDS    RefSeq.
SOURCE      Actinobacillus pleuropneumoniae
  ORGANISM  Actinobacillus pleuropneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Actinobacillus.
REFERENCE   1  (bases 1 to 2310)
  AUTHORS   Dona,V. and Perreten,V.
  TITLE     Complete genome sequences of Actinobacillus pleuropneumoniae strains
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2310)
  AUTHORS   Dona,V. and Perreten,V.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2018) Institute of Veterinary Bacteriology,
            University of Bern, Laenggassstrasse 122, Bern 3012, Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Canu v. 1.3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 241x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_021953265.1-RS_2024_03_17
            Annotation Date                   :: 03/17/2024 03:06:50
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,195
            CDSs (total)                      :: 2,109
            Genes (coding)                    :: 2,066
            CDSs (with protein)               :: 2,066
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 43
            CDSs (without protein)            :: 43
            Pseudo Genes (ambiguous residues) :: 0 of 43
            Pseudo Genes (frameshifted)       :: 24 of 43
            Pseudo Genes (incomplete)         :: 16 of 43
            Pseudo Genes (internal stop)      :: 14 of 43
            Pseudo Genes (multiple problems)  :: 8 of 43
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP031857.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2310
                     /organism="Actinobacillus pleuropneumoniae"
                     /mol_type="genomic DNA"
                     /strain="16287-1"
                     /host="pig"
                     /db_xref="taxon:715"
                     /plasmid="pAP17_1"
     gene            join(2082..2310,1..269)
                     /locus_tag="D1110_RS10970"
                     /old_locus_tag="D1110_11060"
     CDS             join(2082..2310,1..269)
                     /locus_tag="D1110_RS10970"
                     /old_locus_tag="D1110_11060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001027759.1"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA];
                     GO:0006276 - plasmid maintenance [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="WP_001027759.1"
                     /translation="MNITERTDSKKKVLRQNITEVIDRETGNITQEITDITVQFPQEPA
                     YVKIYIDNLCAVTKAPDSLKDVLFLILRKLDYDGYIALSTRYRKEICKLLGIKDGTLRN
                     RLYSLSKMGIIASCGGNEYQANPNLFARGEWKKIIEQRREFEMRIKYSPNGETIITTEE
                     IK"
                     /name="replication/maintenance protein RepL"
     gene            complement(303..728)
                     /gene="mobA"
                     /locus_tag="D1110_RS10975"
                     /old_locus_tag="D1110_11065"
     CDS             complement(303..728)
                     /gene="mobA"
                     /locus_tag="D1110_RS10975"
                     /old_locus_tag="D1110_11065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001107325.1"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein MobA"
                     /protein_id="WP_001107325.1"
                     /translation="MAKNKRSGSEVRQRTKVITLRVNNRVASEIRRRAKNEGLTISEYI
                     RTASLNNEIKQRVPSRYLDELIRLGRMQKKLFDKGKRPKDKEYLEVMHKIILLCDEMKI
                     VTKRISDVYNEMDLIKDEIKIIKRIHKNKYPGSDLFK"
                     /name="mobA"
     Typing          complement(1475..1733)
                     /name="Col(MG828)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="86.415"
                     /coverage="100.0"
                     /evalue="6.900000000000001e-74"
ORIGIN
        1 atgacggata tattgcatta tcaactagat accgtaagga gatctgtaaa ttattgggaa
       61 taaaagacgg aacattacgt aacagattgt attcattatc aaaaatgggg attattgcta
      121 gttgtggcgg taatgaatat caggcgaacc ctaatttatt tgctcgtggt gaatggaaaa
      181 aaataattga acagcgtcgc gagtttgaaa tgagaattaa atattcacct aacggtgaaa
      241 ctattataac aaccgaagaa ataaaataat gtgcacctaa ataaggtctt tttaaaaaga
      301 ccttatttga ataaatccga acctggatat ttatttttat gtatcctttt tattattttt
      361 atttcatcct ttattaaatc catttcatta tatacatcag aaattcgctt cgttactatt
      421 ttcatttcat cgcaaagtaa aattatttta tgcatcacct cgaggtactc cttatccttc
      481 ggccgctttc ccttatcaaa taatttcttc tgcatccgac caagtcggat taattcatct
      541 aaatacctgg aaggtacgcg ctgttttatt tcattattta aacttgccgt cctgatatat
      601 tctgaaattg tcagtccttc gttcttcgct cgccgtctga tttcgcttgc gactctgtta
      661 tttactctaa gggtgataac cttagttctc tgccgtactt ctgaaccact acgtttattt
      721 ttagccatcg caatgactct ccattttgca cgtttggggt gtcggggctt gccctgacag
      781 gaggtattct catgcaagcg aatgtattcg cggtatgacg aatacacatc ctgtccaatt
      841 ttccgctaca acttttcgac gaaaataaaa taccgcgtcg taacctggta tttctgatac
      901 aaaaaaatga tgcttcattc aatattattt acacagattt taaaaattat tgcttgttat
      961 ttaataaata acatcgaatt aattgatgtc tttgttgtct gtcgtttgtg gattattgga
     1021 gggataaaga ttagatcttc ctcgttatat aatccattgc ctgttaatga tacggagtgt
     1081 gtccaaggaa caggaagcgg aagagcaaga tagtagcatg tacataaaat ataaatccgt
     1141 gagttattga tatcgcgttt atatattaaa aatataactt aacattgctc ttaatattta
     1201 ttttgtcctg gcaatgggag cattaatccc cgtgcagtgc tccgccagct gctgacaacc
     1261 tcgacatata accatcgata cgatagaaca tcagggcgcg tttccggcaa gaactaacca
     1321 tggggatggt ttgcgtgccg acaaacgccg gaacgcgcct caaatccacc aagccaccac
     1381 ggttccggca gcgccttgag cgcaaccgga aaccgtctgg ggagaacccc agacccccac
     1441 cgctgcaaca tcatcggtct acgaccacct accaaccgag tggctacctg tgccgtgcga
     1501 acaggctttg cagggggctg gcgcggttcc tggcgacctg aaagcccctg gttaggggta
     1561 tcggcgcgcc tggcctcctg aaaccctctg taagcgtttt tcgcgccttt catgccctga
     1621 cgggcattta aaccccgttt cgtgcgtctg ggaggctctg agcgcgttta acagatgacc
     1681 agcgactcga tcgcctgaga tcaaatcggg atcatcaacg atatgcgaga ggtggcaccg
     1741 ctcgccgcag ccccagcgac cgaacgtagt gagcgagtgg gcgaggaagc ggaagagaac
     1801 cggaagctac attgagcacg cacgctcaat attaaaattg tcacttcgta atatagagat
     1861 tatgcctcgt gcttatggat acatttaaaa atataagata tccgaattag gctacatacg
     1921 ggctgatcgc caatgttgta acgtgtagaa gatacacctg taacgtgtac aggatactcc
     1981 tgtaacgtgt gggcggtatt gtcgtaatct atattgagtg acattttgtt tttttggcat
     2041 tatattgtct gtcaccttta taaagtgaca aaggcgataa aatgaacatt actgaaagaa
     2101 cggatagcaa gaaaaaagtt ttaagacaaa atattactga ggttatagat agagaaactg
     2161 gaaacataac acaagaaata accgatataa cagttcaatt tccacaagaa cctgcgtatg
     2221 tgaaaattta tattgataat ttatgtgctg tcacaaaagc acctgattct ctaaaagatg
     2281 ttttattttt aattcttagg aaattggatt
//
